Publications by authors named "Jiangtao Fan"

24 Publications

  • Page 1 of 1

Letter to editor in response to: seasonal dynamic of cholecalciferol (D3) and anti-Muellerian hormone (AMH) with impact on ovarian response and IVF/ICSI.

Arch Gynecol Obstet 2022 Apr 11. Epub 2022 Apr 11.

Department of Gynecology, Guangxi Medical University First Affiliated Hospital, Nanning, Guangxi, China.

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http://dx.doi.org/10.1007/s00404-022-06568-3DOI Listing
April 2022

Research on Mechanical Properties and Influencing Factors of Cement-Graded Crushed Stone Using Vertical Vibration Compaction.

Materials (Basel) 2022 Mar 14;15(6). Epub 2022 Mar 14.

Key Laboratory for Special Area Highway Engineering of Ministry of Education, Chang'an University, Xi'an 710064, China.

To study the mechanical properties of cement-graded crushed stone for use in the transition sections of intercity railways, the growth laws governing unconfined compressive strength, splitting strength and resilience modulus of cement-graded crushed stone and their influencing factors were studied by the vertical vibration compaction method (VVCM). The strength growth equations of cement-graded crushed stone are proposed, and strength prediction equations are established. The research shows the unconfined compressive strength, splitting strength and resilience modulus of cement-graded crushed stone with a strong interlocked skeleton density type (VGM-30) are significantly enhanced to 20, 20 and 17% higher, respectively, than those of standard cement-graded crushed stone. The growth law of mechanical properties of cement-graded crushed stone is similar, with the fastest growth occurring before 14 days, and the rate decreasing after 28 days. The strength growth tended to be stable after 90 days, increasing with the increase in curing time, compaction coefficient and cement dosage. The correlation coefficients (R) of the strength growth prediction models were found to be 0.99, 0.97, and 0.99, respectively. These values can be used to accurately predict the strength growth curve. This paper verifies the superiority of VGM-30 gradation through laboratory tests, providing a reference for gradation selection in the construction of intercity railway transition sections.
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http://dx.doi.org/10.3390/ma15062132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8955322PMC
March 2022

Giant dielectric response and relaxation behavior in (Tm + Ta) co-doped TiO ceramics.

Phys Chem Chem Phys 2022 Feb 23;24(8):4759-4768. Epub 2022 Feb 23.

Tianjin Key Laboratory of Functional Crystal Materials, Institute of Functional Crystal, College of Material Sciene and Engineering, Tianjin University of Technology, Tianjin 300384, China.

Dielectric materials with huge dielectric constants are attracting attention due to the growing demand for microelectronics and high energy-storage devices. In this work, Tm + Ta co-doped TiO ceramics were prepared by a solid-state reaction (SSR) method, and the microstructure and dielectric behavior were investigated. A ultrahigh permittivity ( ∼ 2.26 × 10) and very low loss (tan  ∼ 0.011) are achieved at 1 kHz for (TmTa)TiO ceramics. XPS analysis confirms that the high dielectric constant and low dielectric loss are attributed to the electron pinned defect dipole (EPDD) response formed by the coupling of Ti and oxygen vacancies. In addition, impedance analysis and frequency dependent dielectric constant under a DC bias indicate that the presence of the internal barrier layer capacitance (IBLC) response and electrode response at low to medium frequencies (<10 Hz) also contribute significantly to the dielectric constant. The findings reported in this work provide valuable insights into the simultaneous realization of a low dielectric loss and high permittivity in Tm + Ta co-doped TiO ceramics and other related dielectric ceramics.
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http://dx.doi.org/10.1039/d1cp05348eDOI Listing
February 2022

The response of three typical freshwater algae to acute acid stress in water.

J Environ Sci Health A Tox Hazard Subst Environ Eng 2022 6;57(2):102-110. Epub 2022 Feb 6.

Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Xi'an University of Architecture and Technology, Xi'an, China.

The effect of acidic pH conditions on the physiological response of three typical freshwater algae, , and , was investigated in this study. The results of the cultivation experiment indicated that the mortality of the three algae in the logarithmic growth phase increased with increasing exposure time and acidity under acute acidic conditions. The tolerance of was stronger than that of the other two species under long-term (6 h) exposure to the same acidity; in contrast, exhibited the greatest tolerance under short-term exposure conditions. The decrease in chlorophyll (Chl ) content indicated that the photosynthesis of algae was inhibited under acid stress and that the algae could not continue to grow normally. This was consistent with the changes in lipid peroxidation and antioxidant enzyme activity, which were reflected by the malondialdehyde (MDA) content and superoxide dismutase (SOD) activity under acid stress, respectively. The results of this study demonstrated that when exposed to acute acidic conditions, the tolerance of three typical freshwater algae to acidity was significantly different. These findings provide valuable information for poorly mixed acidification operations designed to adjust the pH in lakes, reservoirs, or intake pipes of purification plants.
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http://dx.doi.org/10.1080/10934529.2022.2036070DOI Listing
February 2022

Conversion effects of farmland to Zanthoxylum bungeanum plantations on soil organic carbon mineralization in the arid valley of the upper reaches of Yangtze River, China.

PLoS One 2022 4;17(2):e0262961. Epub 2022 Feb 4.

Key Laboratory of National Forestry Administration on Forest Resources Conservation and Ecological Safety in the Upper Reaches of the Yangtze River, College of Forestry, Sichuan Agricultural University, Chengdu, China.

Farmland conversion to forest is considered to be one of the effective measures to mitigate climate change. However, the impact of farmland conversion to forest land or grassland on soil CO2 emission in arid areas is unclear due to the lack of comparative information on soil organic carbon (SOC) mineralization of different conversion types. The SOC mineralization in 0-100 cm soil layer in farmland (FL), abandoned land (AL) and different ages (including 8, 15, 20 and 28 years) of Zanthoxylum bungeanum plantations were measured by laboratory incubation. The size and decomposition rate of fast pool (Cf) and slow pool (Cs) in different land-use types and soil layers were estimated by double exponential model. The results showed that: 1) Farmland conversion increased the cumulative CO2-C release (Cmin) and SOC mineralization efficiency, and those indexes in AL were higher than that in Z. bungeanum plantations. The Cmin and SOC mineralization efficiency of 0-100 cm soil increased with the ages of Z. bungeanum plantation. Both Cmin and SOC mineralization efficiency decreased with the increase of soil depth; 2) Both soil Cf and Cs increased after farmland converted to Z. bungeanum plantations and AL. The Cs in the same soil layer increased with the ages of Z. bungeanum plantation, and the Cf showed a "V" type with the increased ages of Z. bungeanum plantation. The Cf and Cs decreased with the increase of soil depth in all land-use types; 3) Farmland conversion increased the decomposition rate of Cf (k1) in all soil layer by 0.008-0.143 d-1 and 0.082-0.148 d-1 in Z. bungeanum plantations and AL, respectively. The k1 was obviously higher in the 0-20 cm soil layer than that in other soil layers, while the decomposition rate of Cs (k2) was not affected by FL conversion and soil depth; and 4) The initial soil chemical properties and enzyme activity affected SOC mineralization, especially the concentrations of total organic nitrogen (TON), SOC, easily oxidizable organic carbon (EOC) and microbial biomass carbon (MBC). It indicated that the conversion of farmland to Z. bungeanum plantations and AL increases SOC mineralization, especially in deeper soils, and it increased with the ages. The conversion of farmland to Z. bungeanum plantation is the optimal measure when the potential C sequestration of plant-soil system were taken in consideration.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0262961PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815984PMC
February 2022

Circular RNA lysophosphatidic acid receptor 3 (circ-LPAR3) enhances the cisplatin resistance of ovarian cancer.

Bioengineered 2022 02;13(2):3739-3750

Department of Gynecology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Circular RNA (circRNA) is considered to be an important regulator that mediates cancer chemoresistance. But whether circ-LPAR3 is involved in ovarian cancer (OC) cisplatin (DDP) resistance is unclear. The circ-LPAR3, miR-634 and pyruvate dehydrogenase kinase 1 (PDK1) expression was measured by quantitative real-time PCR (qRT-PCR). Cell cisplatin resistance and viability were measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. In addition, cell colony number, apoptosis, and metastasis were assessed by colony formation assay, flow cytometry and transwell assay. Furthermore, experiments were performed by constructing mice xenograft models. RNA interaction was confirmed by dual-luciferase reporter assay, and PDK1 protein expression was examined by Western blot analysis. Our results showed that circ-LPAR3 was markedly upregulated in DDP-resistant OC tissues and cells. Silencing of circ-LPAR3 enhanced the DDP sensitivity of OC cells and tumors. MiR-634 could interact with circ-LPAR3, and its inhibitor overturned the regulation of si-circ-LPAR3 on cell DDP resistance. Additionally, PDK1 was targeted by miR-634, and its overexpression inverted the effect of miR-634 on cell DDP resistance. To sum up, circ-LPAR3 might contribute to the DDP resistance of OC via the miR-634/PDK1 axis.
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http://dx.doi.org/10.1080/21655979.2022.2029109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974081PMC
February 2022

Corrigendum to "Ovarian metastasis risk factors in endometrial carcinoma: A systematic review and meta-analysis" [Eur J Obstet Gynecol Reprod Biol 267 (2021) 245-255].

Eur J Obstet Gynecol Reprod Biol 2022 Feb 29;269:83. Epub 2021 Dec 29.

Department of Gynecology, Guangxi Medical University First Affiliated Hospital, Nanning, Guangxi, China. Electronic address:

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http://dx.doi.org/10.1016/j.ejogrb.2021.12.005DOI Listing
February 2022

The utilization of accurate body mass index classification is imperative for grouping based on BMI.

Hum Reprod 2022 03;37(3):622-623

Department of Gynecology, Guangxi Medical University First Affiliated Hospital, Nanning, China.

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http://dx.doi.org/10.1093/humrep/deab276DOI Listing
March 2022

Ovarian metastasis risk factors in endometrial carcinoma: A systematic review and meta-analysis.

Eur J Obstet Gynecol Reprod Biol 2021 Dec 13;267:245-255. Epub 2021 Nov 13.

Department of Gynecology, Guangxi Medical University First Affiliated Hospital, Nanning, Guangxi, China. Electronic address:

Objective: To investigate the incidence of ovarian metastasis in endometrial carcinoma (EC) and analyze its risk factors and provide a theoretical basis for whether retention of the ovary in patients with EC.

Methods: A systematic search using synonyms of 'ovarian cancer' and 'metastasis' was conducted in PubMed, Cochrane database, Embase, Google Scholar, and WOS database. Meta-analysis was performed on 7 included studies, comprising 4281 clinical-stage I-IV EC patients. Studies were assessed using the Newcastle-Ottawa Scale (NOS) criteria. Odds risks (OR) and 95% confidence intervals (CI) were calculated using an inverse variance weighted random-effects model.

Results: The ovarian metastasis risk of EC was significantly higher for patients with myometrial invasion >1/2 (OR = 18.19, 95% CI 5.34 to 61.96 compared to myometrial invasion ≤1/2), any pelvic lymph node invasion (PLNI) (OR = 5.41, 95% CI 2.60-10.97 compared to without PLNI), G3 pathological grade (OR = 2.66, 95%CI 1.35-5.24 compared to G1-G2), non-endometrioid pathological type (OR = 6.46, 95% CI 3.25 to 12.83 compared to endometrioid), lymphatic vascular space invasion (LVSI) (OR = 6.46, 95% CI 3.25 to 12.83 compare to without LVSI), age >45 (OR = 2.01, 95% CI 0.29 to 14.11 compared to age ≤45), and cervical invasion (OR = 4.12, 95% CI 1.87 to 9.08 compared to without cervical invasion).

Conclusion: About 4.95% of EC patients develop ovarian metastasis. Age >45, myometrial invasion >1/2, cervical invasion, PLNI, pathological type, G3 pathological grade, and LVSI were the high-risk factors for ovarian metastasis of EC. Ovarian preservation should be carefully selected for patients with EC, and preoperative and intraoperative evaluations should be entirely performed.
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http://dx.doi.org/10.1016/j.ejogrb.2021.11.016DOI Listing
December 2021

Silencing YKL-40 gene can inhibit inflammatory factor expression and affects the effect of THP-1 cells on endometrial cancer.

Arch Gynecol Obstet 2022 02 21;305(2):467-473. Epub 2021 Aug 21.

The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, China.

Purpose: To investigate the effect of silencing the YKL-40 gene on the expression of inflammatory factors and the effect of silencing the YKL-40 gene of THP-1 cells on endometrial cancer.

Methods: We used a siRNA targeting a sequence in YKL-40 (si-YKL-40) to transfect HEC-1A and THP-1 cells. Quantitative real-time polymerase chain reaction assay was performed to investigate the mRNA levels of YKL-40, IL-8 and MMP-9 in HEC-1A and THP-1 cells. Migration, and invasion assays were performed to identify the effects of co-culture with THP-1 cells that silenced YKL-40 gene on the migration and invasion capacity of HEC-1A cells. Tube formation ability were detected by Matrigel-based angiogenesis assay.

Results: We successfully transfected HEC-1A and THP-1 cells with lentivirus to silence the YKL-40 gene. Compared with the blank control group and NC group, the expression of YKL-40, IL-8 and MMP-9 which were examined by qRT-PCR in YKL-40-siRNA group was significantly reduced in the two cell lines; after co-cultured with the supernatant of transfected THP-1 cells, the migration and invasion ability of HEC-1A cells in YKL-40-siRNA group was significantly reduced; the number of tubes in the YKL-40-siRNA group was significantly reduced, the spacing between the tubes was significantly increased, and the structure of tubes was incomplete.

Conclusion: Silencing the YKL-40 gene in THP-1 cells can inhibit the expression of inflammatory factors, the invasion and migration of human endometrial cancer cells and the capacity of vitro angiogenic. And YKL-40 gene as a marker of inflammation may be an effective therapeutic target for endometrial cancer.
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http://dx.doi.org/10.1007/s00404-021-06194-5DOI Listing
February 2022

Development and Performance Evaluation of a High-Permeability and High-Bonding Fog-Sealing Adhesive Material.

Materials (Basel) 2021 Jun 28;14(13). Epub 2021 Jun 28.

Key Laboratory for Special Area Highway Engineering of Ministry of Education, Chang'an University, South 2nd Ring Rd., Middle Section, Xi'an 710064, China.

Herein, the effects of the contents of emulsified asphalt, waterborne epoxy resin emulsion, and curing agent on the permeability, bond shear strength, water stability, and aging resistance of epoxy-emulsified asphalt were studied. A formulation of epoxy-emulsified asphalt as a fog-sealing adhesive material was recommended, and a comparison between the fabricated adhesive material and a traditional Chinese fog-sealing adhesive material was conducted to verify the technical performance of the new material. In addition, the strength formation mechanism of the epoxy-emulsified asphalt was revealed via microcosmic analysis. Results show that the curing agent content mainly affects the permeability of epoxy-emulsified asphalt, and the emulsified asphalt content significantly affects the bond shear strength, water stability, and aging resistance. Moreover, the ratio of waterborne epoxy resin emulsion to the curing agent (epoxy ratio) has a certain effect on the bond shear strength. In the recommended formulation (a high-permeability and high-bonding fog-sealing adhesive material, which can be referred to simply as HPBFA), emulsified asphalt accounts for 80% of the total mass of the mixture, and the epoxy ratio is 2:1-3:1. It can improve air permeability, bond shear strength, water stability and aging resistance. The HPBFA-cured material exhibits a continuous three-dimensional network structure, hydrophobic surface, and large contact angle. Furthermore, the initial thermal weight loss temperature of the HPBFA-cured material is significantly higher than the environmental aging temperature. Additionally, the maximum temperature decomposition range is 0-160 °C, indicating improved strength, wear resistance, permeability, and aging resistance of the material.
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http://dx.doi.org/10.3390/ma14133599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269687PMC
June 2021

Investigation on cement-improved phyllite based on the vertical vibration compaction method.

PLoS One 2021 3;16(3):e0247599. Epub 2021 Mar 3.

Key Laboratory for Special Area Highway Engineering of Ministry of Education, Chang' an University, Xi'an, China.

The vertical vibration compaction method (VVCM), heavy compaction method and static pressure method were used to form phyllite specimens with different degrees of weathering. The influence of cement content, compactness, and compaction method on the mechanical properties of phyllite was studied. The mechanical properties of phyllite was evaluated in terms of unconfined compressive strength (Rc) and modulus of resilience (Ec). Further, test roads were paved along an expressway in China to demonstrate the feasibility of the highly weathered phyllite improvement technology. Results show that unweathered phyllite can be used as subgrade filler. In spite of increasing compactness, phyllite with a higher degree of weathering cannot meet the requirements for subgrade filler. With increasing cement content, Rc and Ec of the improved phyllite increases linearly. Rc and Ec increase by at least 15% and 17%, respectively, for every 1% increase in cement content and by at least 10% and 6%, respectively, for every 1% increase in compactness. The higher the degree of weathering of phyllite, the greater the degree of improvement of its mechanical properties.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247599PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7928446PMC
September 2021

Laboratory Evaluation of a Vertical Vibration Testing Method for an SMA-13 Mixture.

Materials (Basel) 2020 Oct 3;13(19). Epub 2020 Oct 3.

College of Transportation, Jilin University, Changchun 130012, China.

In order to simulate the on-site compaction conditions of a Stone Matrix Asphalt (SMA) mixture, The Vertical Vibration Testing Method (VVTM), Superpave Gyratory Compactor (SGC), and Marshall method are used to test the SMA-13 mixture, and the physical and mechanical properties of the asphalt mixture designed by these three methods are tested. Subsequently, the influences of the molding method on the mechanical properties are compared. The influence of vibration compaction time on the volume parameters of the SMA mixture is studied. Following the heavy traffic compaction standards, the vibration compaction time of the SMA mixture is determined. The results show that the densities of the heavy Marshall specimen, VVTM specimen, and SGC specimen are 1.018 times, 1.019 times, and 1.015 times greater than that of the standard Marshall specimen, respectively. The passing rate of the 4.75 mm aggregate of the standard Marshall specimen is 29.9%, and that of the VVTM specimen and SGC specimen is 31.1% and 30.5%, respectively, while that of the heavy Marshall specimen is 34.5%. The mechanical strength of the specimen can be greatly improved as the density increases. On the other hand, by the same compaction work, the mechanical strength of the VVTM specimens can be increased by at least 7% compared with the heavy Marshall specimen. The mechanical strength of the VVTM specimen is increased by at least 22% compared with the standard Marshall specimen. The results also show that under the optimal asphalt-aggregate ratio and the same compaction work, the compressive strength and shear strength of the VVTM specimens are increased by at least 6% and 9%, respectively, compared with the Marshall specimens. In summary, the performance of the asphalt mixture designed by the VVTM is superior, providing a wider choice for future asphalt mixture design.
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http://dx.doi.org/10.3390/ma13194409DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579442PMC
October 2020

Diagnostic value of Anti-Mullerian hormone in ovarian granulosa cell tumor: A meta-analysis.

Eur J Obstet Gynecol Reprod Biol 2020 Oct 22;253:266-272. Epub 2020 Aug 22.

Department of Gynecology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Province, 530022, China. Electronic address:

Background: Granulosa cell tumor (GCT) is a rare type of ovarian malignancy with poor prognosis of recurrence. Anti-Mullerian hormone (AMH) is a promising serum biomarker for early diagnosis GCT, therefore we assessed its accuracy and clinical value.

Methods: A meta-analysis was performed to estimate the diagnostic accuracy. We searched the PubMed, Cochrane Literature Library and EMBASE database. After filtering, five studies met our criteria. Two independent reviewers extracted data and total 421 serum samples (70 GCT serum samples and 351 controls) were included in our analysis. The pooled values of sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR) and Summary receiver operating characteristic (SROC) were calculated to assess the accuracy.

Results: The pooled estimated for AMH were sensitivity: 0.89 (95 % CI 0.78-0.95), specificity: 0.93 (95 %CI 0.83-0.97). The area under the SROC was 0.93 (95 %CI 0.91-0.95).

Conclusion: Serum AMH can be used as a biomarker in clinical diagnosis of GCT with high accuracy.
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http://dx.doi.org/10.1016/j.ejogrb.2020.08.011DOI Listing
October 2020

Long-term variations in fish community structure under multiple stressors in a semi-closed marine ecosystem in the South China Sea.

Sci Total Environ 2020 Nov 17;745:140892. Epub 2020 Jul 17.

South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China; Key Laboratory of Open-Sea Fishery Development, Ministry of Agriculture and Rural Affairs, Guangzhou, China; Southern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China. Electronic address:

Daya Bay is an ecologically and economically crucial semi-closed bay along the southern coast of China. It is proven to be a stressed ecosystem and therefore obviously vulnerable to further extrinsic disturbance. This study used fish data from bottom-trawl surveys, conducted from 1985 to 2018, to analyze variations in the fish community structure over the past 30 years. The results showed that warm-water fish species were overwhelmingly dominant during all years, suggesting the bay's tropical to subtropical characteristic. By 2015, the number of fish species had decreased by 29.44% of that caught in 1987, moreover, values of the Shannon-Wiener diversity index and the Margalef richness index were lower in 2015 compared to 2004. There were evident shifts in the fish community composition from pelagic to demersal species, as suggested by the dominant species found in springtime, the dominant families, and percentages denoting the numbers of species in the main orders. Average fish body weight in landings declined from 13.4 g to 7.58 g, the body sizes of four typical commercial fish species decreased by varying degrees over the last 30 years. Abundance-biomass comparison curves suggested that the Daya Bay fish community was more stressed in 2015 than in 2004 during all seasons, except winter. In general, the fish community structure in Daya Bay is consequently in an unsteady state. Multiple anthropogenic disturbances, such as fishing (including overfishing and changes in the main fishing gears), the destruction of natural habitats, pollutants, and anthropogenically induced temperature changes, are likely to have caused obvious shifts in the bay's fish community structure. Therefore, we emphasize the need for integrating management of multiple anthropogenic stressors to achieve ecosystem-based management.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140892DOI Listing
November 2020

Genomic analysis provides insights into the transmission and pathogenicity of Talaromyces marneffei.

Fungal Genet Biol 2019 09 7;130:54-61. Epub 2019 May 7.

Department of Respiratory Medicine, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. Electronic address:

Talaromyces marneffei (T. marneffei) is a medically important opportunistic dimorphic fungus that infects both humans and bamboo rats. However, the mechanisms of transmission and pathogenicity of T. marneffei are poorly understood. In our study, we combined Illumina and PacBio sequencing technologies to sequence and assemble a complete genome of T. marneffei. To elucidate the transmission route and source, we sequenced three additional T. marneffei isolates using Illumina sequencing technology. Variations among isolates were used to develop a multilocus sequence typing (MLST) system comprising five housekeeping genes that can be used to discriminate between isolates derived from different sources. Our analysis revealed that human and bamboo rat share identical genotypes in these five loci. Thus, we hypothesized that T. marneffei is transmitted to humans through inhalation of spores in the surrounding environment into the lungs and that the bamboo rat can serve as an important natural reservoir for pathogens. Furthermore, we also identified temperature-dependent polyketide synthases, non-ribosomal peptide synthetases and secreted proteins as putative pathogenicity-related factors. In addition, we identified antifungal drug targets that can be investigated in future studies to elucidate the mechanisms underlying drug resistance. In summary, our study presents the basic features of the T. marneffei genome and provides insights into the transmission and pathogenicity of T. marneffei, which warrant fundamental experimental research.
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http://dx.doi.org/10.1016/j.fgb.2019.05.002DOI Listing
September 2019

Defective [email protected] hybrid nanocarbon material as an effective and stable metal-free catalyst for acetylene hydrochlorination.

Chem Commun (Camb) 2019 Jan;55(10):1430-1433

Institute of Industrial Catalysis, Zhejiang University of Technology, Chaowang Road 18, Hangzhou, China.

A defective nanodiamond-graphene material ([email protected]) exhibits superior catalytic activity in acetylene hydrochlorination with an acetylene conversion of 50%, selectivity to vinyl chloride of up to 99.5% at 220 °C, and a C2H2 gas hourly space velocity of 300 h-1, which is the first example of a metal-free catalyst with comparable performance to that of the 0.25% Au/C catalyst.
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http://dx.doi.org/10.1039/c8cc09361jDOI Listing
January 2019

Influence of YKL-40 gene RNA interference on the biological behaviors of endometrial cancer HEC-1A cells.

Oncol Lett 2018 Aug 25;16(2):1777-1784. Epub 2018 May 25.

Department of Gynecology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

The present study aimed to investigate the effects of chitinase-3-like protein 1 (YKL-40) gene RNA interference on the biological behaviors and enhanced chemosensitivity of endometrial cancer (EC) HEC-1A cells. YKL-40 small interfering (si)RNA was transduced into EC HEC-1A cells using a lentivirus. The experiment was divided into three groups: The experimental group was transfected with YKL-40 siRNA (si-YKL-40); the mock-treatment group was transfected with transfection reagent only; and the blank control group was left untreated. A reverse transcription-quantitative polymerase chain reaction was performed to investigate the mRNA expression levels of YKL-40. The biological behaviors, including cell proliferation, migration, invasion and apoptosis, were detected by MTT and Transwell assays, and flow cytometry (FCM) analysis, respectively. The results of the present study demonstrated that the mRNA expression levels of YKL-40 were downregulated within HEC-1A cells upon transfection with si-YKL-40 (P<0.05). The proliferative, migratory and invasive abilities of HEC-1A cells were inhibited by si-YKL-40 (P<0.05). The mRNA expression levels of YKL-40 were upregulated within HEC-1A cells following treatment with cisplatin (P<0.05). FCM analysis revealed that the average cellular apoptosis rate increased following the inhibition of YKL-40 gene expression via siRNA (P<0.05). Therefore, the YKL-40 gene may be associated with the proliferative, migratory, invasive and anti-apoptotic ability of HEC-1A cells. YKL-40 downregulation may enhance the sensitivity of human EC HEC-1A cells to chemotherapy.
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http://dx.doi.org/10.3892/ol.2018.8814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036507PMC
August 2018

Acute Penicillium marneffei infection stimulates host M1/M2a macrophages polarization in BALB/C mice.

BMC Microbiol 2017 Aug 18;17(1):177. Epub 2017 Aug 18.

Department of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, China.

Background: Penicillium marneffei (P. marneffei) is a thermally dimorphic fungus pathogen that causes fatal infection. Alveolar macrophages are innate immune cells that have critical roles in protection against pulmonary fungal pathogens and the macrophage polarization state has the potential to be a deciding factor in disease progression or resolution. The aim of this study was to investigate mouse alveolar macrophage polarization states during P. marneffei infection.

Results: We used enzyme-linked immunosorbent (ELISA) assays, quantitative real-time PCR (qRT-PCR), and Griess, arginase activity to evaluate the phenotypic markers of alveolar macrophages from BALB/C mice infected with P. marneffei. We then treated alveolar macrophages from infected mice with P. marneffei cytoplasmic yeast antigen (CYA) and investigated alveolar macrophage phenotypic markers in order to identify macrophage polarization in response to P. marneffei antigens. Our results showed: i) P. marneffei infection significantly enhanced the expression of classically activated macrophage (M1)-phenotypic markers (inducible nitric oxide synthase [iNOS] mRNA, nitric oxide [NO], interleukin-12 [IL-12], tumor necrosis factor-alpha [TNF-α]) and alternatively activated macrophage (M2a)-phenotypic markers (arginase1 [Arg1] mRNA, urea) during the second week post-infection. This significantly decreased during the fourth week post-infection. ii) During P. marneffei infection, CYA stimulation also significantly enhanced the expression of M1 and M2a-phenotypic markers, consistent with the results for P. marneffei infection and CYA stimulation preferentially induced M1 subtype.

Conclusions: The data from the current study demonstrated that alveolar macrophage M1/M2a subtypes were present in host defense against acute P. marneffei infection and that CYA could mimic P. marneffei to induce a host immune response with enhanced M1 subtype. This could be useful for investigating the enhancement of host anti-P. marneffei immune responses and to provide novel ideas for prevention of P. marneffei-infection.
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http://dx.doi.org/10.1186/s12866-017-1086-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563047PMC
August 2017

Comparison of the short-term and long-term outcomes of laparoscopic hysterectomies and of abdominal hysterectomies: a case study of 4,895 patients in the Guangxi Zhuang Autonomous Region, China.

Chin J Cancer Res 2016 Apr;28(2):187-96

Department of Gynecological Oncology, Cancer Institute and Hospital of Guangxi Medical University, Nanning 530021, China;

Background: To evaluate the short-term and long-term outcomes after laparoscopic hysterectomy (LH) compared with abdominal hysterectomy (AH) in case of benign gynecological disease.

Methods: A multi-center cohort retrospective comparative study of population among 4,895 hysterectomies (3,539 LH vs.1,356 AH) between 2007 and 2013 was involved. Operative time (OT), estimated blood loss (EBL), intra-operative and post-operative complications, passing flatus; days with indwelling catheter, questionnaires covering pelvic floor functions and sexual functions were assessed.

Results: The EBL (174.1±157.4 vs. 263.1±183.2 cc, LH and AH groups, respectively), passing flatus (38.7±14.1 vs. 48.1±13.2 hours), days with indwelling catheter (1.5±0.6 vs. 2.2±0.8 days), use of analgesics (6.5% vs. 73.1%), intra-operative complication rate (2.4% vs. 4.1%), post-operative complication rate (2.3% vs. 5.7%), post-operative constipation (12.1% vs. 24.6%), mild and serious stress urinary incontinence (SUI) post-operative (P<0.001; P=0.014), and proportion of Female Sexual Functioning Index (FSFI) total score <26.55 post-operative (P<0.001) of the LH group were significantly less than those of AH group. There were no significant differences in OT (106.5±34.5 vs. 106.2±40.3 min) between the two groups.

Conclusions: LH is a safe and efficient operation for improving patients?long-term quality of life (QoL), and LH is a cost-effectiveness procedure for treating benign gynecological disease. LH is superior to AH due to reduced EBL, reduced post-operative pain and earlier passing flatus.
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http://dx.doi.org/10.21147/j.issn.1000-9604.2016.02.06DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865611PMC
April 2016

[Comparison of the short-term and long-term outcomes after laparoscopic surgery for early-stage endometrial cancer].

Zhonghua Fu Chan Ke Za Zhi 2015 Dec;50(12):902-9

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Objective: To evaluate the short- term and long- term outcomes after laparoscopic surgery compared with traditional laparotomy in patients with stage I-II endometrial cancer.

Methods: A retrospective study of population among 673 patientsfor early-stage endometrial cancer between Jan. 2007 and May 2014 was involved from 6 third-grade class-A communal hospitals in Guangxi. Three hundred and seventy-six cases were performed by laparoscopy, 297 cases by laparotomy. The t-test and χ(2) test was used to compare the short-term and long-term outcomes. The short-term outcomes including surgical related outcomes and operative complications, the long- term outcomes including quality of life (pelvic floor functions and sexual functions), survival analysis and recurrence. The International Consultation on Incontinence Questionnaire Female Lower Urinary Tract Sympotom (ICIQ- FLUTS) and the Female Sexual Function Index (FSFI) were used to assess pelvic floor function and sexual function. Survival rates were estimated by Kaplan-Meier analysis. The survival curves were compared by log-rank test. Cox regression analysis was used to select the risk factors for prognosis.

Results: (1) The short-term outcomes: There were significant difference in operative time [(258±71) vs (226±69) minutes], estimated blood loss [(343± 211) vs (491±411) ml], anus exhausting time [(2.3±0.9) vs (2.9±1.0) days], preserved days of installing catheter [(7 ± 5) vs (10±8) days], post- operative length of stay [(12 ± 7) vs (18 ± 12) days] between laparoscopic group andlaparotomy group (all P <0.05). While, there was no significant difference in lymph nodes yielded (21±8 vs 21±11; P>0.05),the intra-operative complications occurred [8.5%(32/376) vs 10.4%(31/297); P>0.05], and the post-operative complications [18.1% (68/376) vs 22.2% (66/297); P>0.05] between laparoscopic group and laparotomy group. However, the complications of vascular injury and the poor wound healing in laparoscopic group were respectively lower than those in laparotomy group [1.9%(7/376) vs 5.4% (16/297), P=0.003; and 0.3% (1/376) vs 4.7% (14/297), P<0.01]. (2) The long- term outcomes: There were no significant differences in overall survival (OS) and the degree of incontinence in ICIQ-FLUTS questionnaire between the two groups (all P >0.05). The sexual desire and sexual satisfaction scores dimension after 12 months of post- operative in FSFI questionnaire in the laparoscopic group were higher than those in laparotomy group (all P <0.05). However, there were no significant differences in sexual arousal, vaginal lubrication, orgasm and sexual pain dimension scores between the two groups (all P >0.05). The recurrence rate was 12.0%(45/376) in laparoscopic group and 14.5%(43/297) in laparotomy group (P= 0.269). The 5-year OS was 89.5% in the laparoscopic group and 87.2% in the open group (P >0.05) , and the 5-year free-progression survival rate was 87.9% in the laparoscopic group and 85.1% in the open group (P >0.05). (3) Prognostic factors in laparoscopic group: The univariate analysis shown that pathological type, surgical pathological staging, deep myometrial invasion, and retroperitoneal lymph node-positive were significantly affected prognosis in laparoscopic group (all P<0.01). The multivariate analyses showed that pathological type and surgical pathological stage were the independent prognostic factors (all P<0.01).

Conclusions: Laparoscopy could reduce estimated blood loss, accelerate postoperative recovery and improve the quality of life after surgery compared to laparotomy, also ensure the same oncologically results as that by laparotomy. So, laparoscopic approach is a safe and effective treatment method for early- stage endometrial cancer.
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December 2015

Whole mitogenome of the Japanese scad Decapterus maruadsi (Perciformes: Carangidae).

Mitochondrial DNA A DNA Mapp Seq Anal 2016 12;27(1):306-7. Epub 2014 Mar 12.

a South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science , Guangzhou , P.R. China .

The Japanese scad (Decapterus maruadsi) is one of the most commercially important fish species that is popularly harvested in tropical and temperate seas in South East Asian countries. In the present study, we obtained the whole mitochondrial genome sequence of D. maruadsi by overlapped polymerase chain reactions. The entire sequence was 16,541 bp in length, with a gene content (13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 non-coding regions: the control region and the light strand replication origin) and organization similar to most of the other vertebrates. Overall base composition of the genome was 27.5% of A, 30.2% of C, 25.5% of T and 16.8% of G, showing an obvious anti G bias commonly observed in teleosts. The sequence data of D. maruadsi could provide useful information for the studies on conservation genetics and molecular phylogenetics.
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http://dx.doi.org/10.3109/19401736.2014.892089DOI Listing
October 2016

Characterization of the mitochondrial genome of the Shortfin scad Decapterus macrosoma (Perciformes: Carangidae).

Mitochondrial DNA A DNA Mapp Seq Anal 2016 17;27(1):82-3. Epub 2014 Jan 17.

a South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science , Guangzhou , PR China .

The complete mitochondrial genome sequence of Decapterus macrosoma has been obtained with overlapped polymerase chain reaction. The genome was 16,545 bp in length which contained 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 2 non-coding regions (the control region and the light strand replication origin). Gene organization was similar to that observed in most other vertebrates. With the exception of the ND6 and eight tRNA genes, all other genes were encoded on the heavy strand. The genome was composed of 27.0% A, 30.4% C, 25.4% T and 17.2% G, showing an obvious anti-G bias commonly observed in teleosts. Two termination-associated sequence regions (TAS-1 and TAS-2), three central conserved sequence blocks (CSB-F, CSB-E and CSB-D), and three conserved sequence blocks (CSB-1, CSB-2 and CSB-3) were identified within the control region. The sequence data of D. macrosoma could provide useful information for the studies on molecular systematics and population genetics.
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http://dx.doi.org/10.3109/19401736.2013.873910DOI Listing
September 2016

An exploratory analysis of γ-synuclein expression in endometrioid endometrial cancer.

BMJ Open 2012 24;2(2):e000611. Epub 2012 Apr 24.

Department of Gynecology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Province, People's Republic of China.

Objective: This study aims to investigate the expression of γ-synuclein in endometrioid endometrial carcinoma and assess if the γ-synuclein expression correlates with the aggression of the tumour and its prognostic value in endometrioid endometrial carcinoma.

Design: This retrospective study evaluated (60) specimens of the primary untreated endometrioid endometrial carcinoma and (12) normal endometrium tissues, and the expression of γ-synuclein was checked by immunohistochemistry. The correlation between γ-synuclein expression and the clinicopathological features of patients with endometrioid endometrial carcinoma was analysed, and SPSS V.13.0 software was used for statistical analysis.

Results: The expression of γ-synuclein was positive in 48.3% (29/60) endometrioid endometrial carcinomas compared with the control group, and the difference was significant (p=0.001). The expression level of γ-synuclein in endometrioid endometrial carcinoma was closely associated with FIGO (International Federation of Gynecology and Obstetrics) stages, the depth of myometrial invasion and lymph nodes metastases (p<0.05), but not correlated with the histopathological grades, the patient's age and the expression of ER (estrogen receptor) and PR (progesterone receptor) (p>0.05). In univariate and multivariate analyses, the γ-synuclein expression was significantly associated with a shorter overall survival (95% CI 1.429 to 101.892, p=0.020).

Conclusions: This study suggests that the expression of γ-synuclein is expected to be a useful marker for endometrioid endometrial carcinoma invasion, metastasis and prognosis in endometrioid endometrial carcinoma.
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http://dx.doi.org/10.1136/bmjopen-2011-000611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3341596PMC
October 2012
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