Publications by authors named "Jiang-Hua Lai"

23 Publications

  • Page 1 of 1

Cellular Inflammatory Response of the Spleen After Acute Spinal Cord Injury in Rat.

Inflammation 2019 Oct;42(5):1630-1640

Department of Orthopaedics, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, 710004, China.

Spinal cord injury (SCI) involves both primary and secondary damages. After the phase of primary injury, a series of inflammatory responses initiate, which belong to the secondary injury. There has been little investigation into the cellular inflammatory response of the spleen to SCI. To disclose the impact of SCI on the spleen, we examined the inflammatory reactions of the spleen during the acute phase of SCI in rat. Adult rats were used as experimental animals and divided into un-injured, sham, and SCI groups (n = 36). Contusion injuries were produced at the T3 vertebral level. Spinal cords were harvested 6 h, 24 h, 48 h, 72 h, 120 h, and 168 h after surgery and were prepared for immunohistochemistry. Spleen wet weight was measured. Blood and spleens were prepared for quantitative analyses. The spleen index was significantly decreased in the SCI groups. Immunohistochemical results showed an increase of the infiltrating cells in the spinal cord tissues from SCI rats at all time points, peaking in 72 h post injury. In the blood, T and B lymphocytes significantly decreased in the SCI group as compared with the sham group, while monocyte increased. Surprisingly, in the SCI group, neutrophil initially decreased and subsequently tended to return toward baseline levels, then remained elevated until the end of the study. Spleen analyses revealed a significant increase in monocyte and neutrophil but a minor (not statistically significant) reduction in T and B lymphocytes. Our data show that the four most prevalent inflammatory cells infiltrate the spinal cord after injury. Increased levels of inflammatory cells (monocyte and neutrophil) in the blood and spleen appear to be very sensitive to SCI. The spleen plays a critical role in the acute phase of SCI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10753-019-01024-yDOI Listing
October 2019

Expression of NG2 and platelet-derived growth factor receptor alpha in the developing neonatal rat brain.

Neural Regen Res 2017 Nov;12(11):1843-1852

College of Forensic Science, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.

Platelet-derived growth factor receptor alpha (PDGFRα) is a marker of oligodendrocyte precursor cells in the central nervous system. NG2 is also considered a marker of oligodendrocyte precursor cells. However, whether there are differences in the distribution and morphology of oligodendrocyte precursor cells labeled by NG2 or PDGFRα in the developing neonatal rat brain remains unclear. In this study, by immunohistochemical staining, NG2 positive (NG2) cells were ubiquitous in the molecular layer, external pyramidal layer, internal pyramidal layer, and polymorphic layer of the cerebral cortex, and corpus callosum, external capsule, piriform cortex, and medial septal nucleus. NG2 cells were stellate or fusiform in shape with long processes that were progressively decreased and shortened over the course of brain development. The distribution and morphology of PDGFRα positive (PDGFRα) cells were coincident with NG2 cells. The colocalization of NG2 and PDGFRα in the cell bodies and processes of some cells was confirmed by double immunofluorescence labeling. Moreover, cells double-labeled for NG2 and PDGFRα were predominantly in the early postnatal stage of development. The numbers of NG2/PDGFRα cells and PDGFRα cells decreased, but the number of NG2 cells increased from postnatal days 3 to 14 in the developing brain. In addition, amoeboid microglial cells of the corpus callosum, newborn brain macrophages in the normal developing brain, did not express NG2 or PDGFRα, but NG2 expression was detected in amoeboid microglia after hypoxia. The present results suggest that NG2 and PDGFRα are specific markers of oligodendrocyte precursor cells at different stages during early development. Additionally, the NG2 protein is involved in inflammatory and pathological processes of amoeboid microglial cells.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4103/1673-5374.219045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745838PMC
November 2017

Contents of Heavy Metals in Chinese Edible Herbs: Evidence from a Case Study of Epimedii Folium.

Biol Trace Elem Res 2018 Mar 16;182(1):159-168. Epub 2017 Jun 16.

Key Laboratory of Trace Elements and Endemic Diseases of Ministry of Health, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.

Toxic heavy metal contamination in Chinese edible herbs has raised a worldwide concern. In this study, heavy metals in Epimedii Folium, an edible medicinal plant in China, were quantitatively analyzed. Variations of heavy metals in different species, in various organs (i.e., leaves, stems, and roots), in wild-growing and cultivated plants, and in 35 market samples of Epimedii Folium, were systematically investigated. In all of Epimedium samples, Hg (mercury) was not detectable (0.00 μg/g). Four species, Epimedium pubescens, Epimedium sagittatum, Epimedium brevicornu, and Epimedium wushanense, were found to contain Cu (copper) and Pb (lead). And contents of Cu and Pb in E. brevicornu were significantly higher than those in other species (P < 0.01). In wild-growing and cultivated Epimedium plants, Cd (cadmium) and As (arsenic) were not detectable, and concentrations of Cu and Pb in wild-growing plants were significantly higher than those in cultivated plants (P < 0.01). Cd was not detectable in leaves, roots, and stems, while organ specificity was apparent in the distribution of Cu, As, and Pb. And the highest levels of Cu and Pb were observed in roots and leaves, respectively. In Chinese markets, several samples of Epimedii Folium contained excessive Cu, Cd, As, and Pb beyond the national permissible limits. In summary, there was a large variation of heavy metals among Epimedii Folium samples, and Cu and Pb were the most important heavy metals contaminating the edible medicinal plant. Application of Epimedii Folium to drug and food industries will need to focus more on toxic heavy metal contamination.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12011-017-1075-2DOI Listing
March 2018

Analysis of variations in the glutamate receptor, N-methyl D-aspartate 2A (GRIN2A) gene reveals their relative importance as genetic susceptibility factors for heroin addiction.

PLoS One 2013 5;8(8):e70817. Epub 2013 Aug 5.

College of Forensic Science, Xi'an Jiaotong University, Key Laboratory of Ministry of Public Health for Forensic Science, Xi'an, Shaanxi, PR China.

The glutamate receptor, N-methyl D-aspartate 2A (GRIN2A) gene that encodes the 2A subunit of the N-methyl D-aspartate (NMDA) receptor was recently shown to be involved in the development of opiate addiction. Genetic polymorphisms in GRIN2A have a plausible role in modulating the risk of heroin addiction. An association of GRIN2A single-nucleotide polymorphisms (SNPs) with heroin addiction was found earlier in African Americans. To identify markers that contribute to the genetic susceptibility to heroin addiction, we examined the potential association between heroin addiction and forty polymorphisms of the GRIN2A gene using the MassARRAY system and GeneScan in this study. The frequency of the (GT)26 repeats (rs3219790) in the heroin addiction group was significantly higher than that in the control group (χ(2) = 5.360, P = 0.021). The allele frequencies of three polymorphisms (rs1102972, rs1650420, and rs3104703 in intron 3) were strongly associated with heroin addiction (P<0.001, 0.0002, and <0.001, after Bonferroni correction). Three additional SNPs from the same intron (rs1071502, rs6497730, and rs1070487) had nominally significant P values for association (P<0.05), but did not pass the threshold value. Haplotype analysis revealed that the G-C-T-C-C-T-A (block 6) and T-T (block 10) haplotypes of the GRIN2A gene displayed a protective effect (P = <0.001 and 0.003). These findings point to a role for GRIN2A polymorphisms in heroin addiction among the Han Chinese from Shaanxi province, and may be informative for future genetic or neurobiological studies on heroin addiction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0070817PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733659PMC
March 2014

Association of TNF-α and Fas gene promoter polymorphism with the risk of Kashin-Beck disease in Northwest Chinese population.

Clin Rheumatol 2012 Jul 20;31(7):1051-7. Epub 2012 Mar 20.

Faculty of Public Health, College of Medicine, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an Jiaotong University, No. 76 Yanta West Road, Xi'an, Shaanxi 710061, People's Republic of China.

The objective of this study is to investigate the relationship between single-nucleotide polymorphisms (SNPs) of the tumor necrosis factor-α (TNF-α) and Fas genes and Kashin-Beck disease (KBD) in Shaanxi province, Northwest in China. Blood samples of 388 residents were collected from 14 KBD villages in Linyou and Yongshou counties, Shaanxi, Northern of China. One hundred eighty-six cases with KBD and 202 cases of health in KBD areas were diagnosed by "Diagnosis Criterion of Kashin-Beck disease in China (WS/T207- 2010)". The TNF-α -308G/A, TNF-α -238G/A, and Fas -670A/G SNPs were determined by polymerase chain reaction-restriction fragment length polymorphism in combination with sequence analysis in KBD and healthy control groups. The genotypes and allele frequencies distribution of these SNPs were then analyzed. TNF-α -308A allele frequency in KBD patients were significantly higher than that in healthy controls. Although TNF-α -238 genotypes and allele frequencies were not significantly different between KBD patients and the healthy controls, GA genotype and A allele frequency in KBD patients were higher than those in healthy controls. The TNF-α -308G/A SNPs were associated with the susceptibility of KBD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10067-012-1975-7DOI Listing
July 2012

[Association between polymorphism in DVWA and IL-1beta and Kashin-Beck disease].

Sichuan Da Xue Xue Bao Yi Xue Ban 2010 Jul;41(4):669-73

Key laboratory of Environment and Genes Related to Diseases of Ministry of Education, Faculty of Public Health, Medicine College, Xi'an Jiaotong University, Xi'an 710061, China.

Objective: To investigate the association between IL-1beta and DVWA gene and Kashin-Beck disease (KBD).

Methods: Peripheral genomic DNA were extracted from 105 patients with KBD and 98 healthy controls. PCR-RFLP were performed to detect SNP loci of IL-1beta gene and DVWA gene.

Results: The patients with KBD had significantly higher frequency of rs16944 (IL-1beta) locus (chi2 = 24.28, P < 0.001) and single allele frequency of rs16944 (chi2 = 5.683, P = 0.0171) than the healthy controls. There were no significant differences in genotype frequencies,single allele frequencies and haplotypes in rs4685241 and rs1143627 between the patients with KBD and the healthy controls.

Conclusion: rs16944 (IL-1beta) is associated with KBD.
View Article and Find Full Text PDF

Download full-text PDF

Source
July 2010

[Genetic structure of X-STR loci in Bai, Dai and Yi ethnic groups and their affinity with five major populations of China].

Yi Chuan 2009 Feb;31(2):153-9

State Key Subject for Forensic Sciences, the Key Laboratory of National Ministry of Health for Forensic Sciences, College of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.

To determine the genetic polymorphism of three X-STR loci for Bai, Dai, Yi ethnic groups from Yunnan Province, DXS6804, DXS6799 and DXS7132 were genotyped by multiplex PCR and Genscan. Eighteen alleles and thirty-eight genotypes were detected in 89 Bai unrelated persons. The gene frequencies ranged from 0.0200 to 0.6400, and the geno-types frequencies ranged from 0.0256 to 0.3333. Seventeen alleles and twenty-four genotypes were detected in 100 Dai unrelated persons, with the gene frequencies ranging from 0.0135 to 0.7500 and the genotypes frequencies ranging from 0.0385 to 0.5769 respectively. There were 20 alleles and 35 genotypes detected in 88 Yi unrelated persons. The gene frequencies ranged from 0.0125 to 0.5875, and the genotypes frequencies ranged from 0.0250 to 0.3500. The genetic information demonstrated that the three loci are highly polymorphisms in Bai, Dai, Yi ethnic groups. Cluster analysis and phylogenic tree showed the genetic affinity between Bai, Dai, Yi, and Tibet populations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3724/sp.j.1005.2009.00153DOI Listing
February 2009

[Genetic polymorphisms and application of 9 STR loci of 5 ethnic groups in Gansu and Qinghai].

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2006 Dec;31(6):877-82

Department of Forensic Science, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.

Objective: To examine the genetic polymorphism of 9 STR loci in 5 ethnic groups (including Tu, Sala, Dongxiang, Baoan and Yugu) in Gansu and Qinghai, and to evaluate its application.

Methods: Nine STR loci (D3S1358, FGA, TH01, D7S820, VWA, CSF1PO, D5S818, D13S317 and TPOX) were selected as genetic markers. With STR compound amplification and genescan methods, in which STR loci were marked by fluorescence, the genotype of 5 ethnic groups were examined in 606 unrelated individuals by ABI 377 sequencer. These parameters, such as polymorphism information content (PIC), heterozygosity (H), discrimination power (DP) and probability of paternity exclusion (PPE) were calculated.

Results: The genotype frequencies of the 9 STR loci were in accordance with Hardy-Weinberg equilibrium. PIC was within 0.6054 - 0.8735, H was within 0.6158 - 0.8736, DP was within 0.7964 - 0.9691, and PPE was within 0.4610 - 0.8838. Cluster analysis based on allele frequencies in genesis showed Tu, Sala, Dongxiang and Baoan ethnic groups were very close, but Yugu was a little bit far. There were obvious gene exchanges among the populations in north and south of China.

Conclusion: All the 9 STR loci are highly polymorphic in the 5 ethnic groups, which can be useful genetic markers in forensic medicine and population genetics.
View Article and Find Full Text PDF

Download full-text PDF

Source
December 2006

[Analysis of allele frequencies of 6 short tandem repeat loci on chromosome 12 in patients with Kashing-Beck disease].

Nan Fang Yi Ke Da Xue Xue Bao 2006 Apr;26(4):414-7

Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xi'an Jiaotong University, Xi'an 710061, China.

Objective: To analyze the allele frequencies of 6 STR loci (D12S358, D12S1675, D12S1663, D12S1697, D12S16725 and D12S1613) on chromosome 12 among KBD patients and residents in the KBD and non-KBD areas.

Methods: EDTA-blood samples were collected from 146 unrelated Chinese Han individuals in Shaanxi Province including 57 KBD patients, 48 control subjects living in the Kashing-Beck disease(KBD) area and 48 in the non-KBD area. The DNA samples were extracted and amplified by PCR, and the PCR products were analyzed by ABI 3100 Genetic Analyzer.

Results: In KBD patients, the allele number for the 6 STR loci (D12S358, D12S1675, D12S1663, D12S1697, D12S16725 and D12S1613) was 7, 7, 7, 10, 12 and 8, and the genotype number were 13, 12, 9, 17, 19 and 10, respectively; in the residents in KBD area, the allele number was 7, 5, 7, 9, 13 and 9, and the genotype number 12, 10, 12, 19, 16 and 8; in residents in non-KBD area, the allele number was 7, 5, 5, 12, 8 and 9, and the genotype number 17, 16, 8, 22, 14 and 8. There were significant differences in the allele frequencies in the D12S1725 loci between KBD patients and residents living in KBD area (P=0.0119) and the non-KBD area (P=0.0050), but no significant difference in other 5 loci among the 3 groups.

Conclusion: KBD patients have significantly different allele distribution patterns in the D12S1725 loci from the control subjects.
View Article and Find Full Text PDF

Download full-text PDF

Source
April 2006

[The genetic polymorphism of 9 short tandem repeat loci in Yi ethnic group of Yunnan in China].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2006 Apr;23(2):216-8

The State Key Subject for Forensic Sciences, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 P.R.China.

Objective: To study the short teadem repeat(STR) genetics structure of a Chinese Yunnan Yi racial group.

Methods: Genetic distributions for nine STR loci were determined based on STR gene scan marked by fluorescence.

Results: Sixty-nine alleles and 164 kinds of genotypes were detected and identified from 84 unrelated Yi racial individuals. The corresponding gene and genotype frequencies were in 0.0060-0.5060 or 0.0119-0.4167 respectively. The expected and observed genotype frequencies of nine STR loci were in accordance with the Hardy-Weinberg equilibrium(P>0.05). The statistical analyses of nine STR loci showed that PIC was distributed in 0.5804-0.8777, H was in 0.6507-0.8002, DP was in 0.7976-0.9558, EPP was in 0.5207-0.8386, except TPOX and THO1 loci.

Conclusion: Above research data enrich the Chinese genetic database, and play an important role in Chinese genetic study and in forensic application.
View Article and Find Full Text PDF

Download full-text PDF

Source
April 2006

[Analysis of genetic polymorphism of 7 STR loci on chromosome 12 in Shaanxi Han populations].

Yi Chuan 2005 Nov;27(6):869-72

Key laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University Ministry of Education, Xi'an Shaanxi 710061, China.

To analyze the genetic polymorphism of 7 STR loci (D12S1718,D12S1675, D12S358, D12S367, D12S1638, D12S1646 and D12S1682) on chromosome 12 in Shaanxi Hans. EDTA-blood specimens were collected from 80 unrelated individuals from Chinese Han population in Shaanxi province. The DNA samples were extracted and relevant fragments were amplified by polymerase chain reaction (PCR). The PCR products were analyzed by ABI 3100 Genetic Analyzer. The number of alleles and genotypes observed at loci D12S1718, D12S1675, D12S358, D12S367, D12S1638, D12S1646 and D12S1682 were 7, 10, 8, 8, 6, 9, 11 for alleles and 10, 17, 18, 18, 14, 18, and 26 for genotypes, respectively. The heterozygosities for the 7 STR loci were 44.28%, 66.10%, 78.89%, 77.89%, 73.69%, 74.55% and 82.39%, respectively. The distribution of allele frequencies of 7 STR loci on chromosome 12 was consistent with Hardy-Weinberg equilibrium and relatively high genetic polymorphism was observed in Shaanxi Han population.
View Article and Find Full Text PDF

Download full-text PDF

Source
November 2005

[Study on 9 STR loci polymorphism from Chinese Yao ethnic group(Guangxi)].

Yi Chuan 2002 Sep;24(5):537-8

National Laboratory of Forensic Sciences, Xian Jiaotong University, Xi'an 710061, China.

Genetic polymorphism of nine STR loci was investigated from a Chinese Yao population based on STR Genescan. Sixty one alleles was determined for 9 loci, such as D3S1358,vWA,FGA,THO1,TPOX,CSF1P0,D5S818,D13S317 and D7S820 with their frequencies 0.0054-0.5924. The average heterozygosity(H) was 0.7357, polymorphism information content(PIC) was 0.6887, the accumulative discrimination power(DP) was 2.02x10(-10) and the probability of paternity exclusion(PPE) was 0.9999. These results suggested that the nine STR loci are very useful for human identification, such as analyzing forensic casework,establishing DNA databases, processing paternity test and studying gene natural resources.
View Article and Find Full Text PDF

Download full-text PDF

Source
September 2002

[Genetic polymorphisms of 9 STR loci in Dongxiang ethnic group of China].

Yi Chuan 2002 May;24(3):247-50

Forensic Medicine Department of Medical College of Xi'an Jiaotong University, Xian 710061, China.

Genetic distribution for nine STR loci was determined in a Chinese Dongxing ethnic group based on STR genescan marked by fluorescence. Seventy-two alleles and 182 genotypes were observed in 94 unrelated Chinese Dongxiang individuals,with the corresponding gene frequency and genotype frequency being 0.0053-0.5825 and 0.0106-0.2660 respectively. The genotypes of nine STR loci were in accordance with the Hardy-Weinberg equilibrium (P>0.05). The statistical analysis of nine STR loci showed PIC (polymorphism information content, PIC) = or > 0.6378, H(heterozygosity, H) = or > 0.6500, DP (discrimination power, DP) = or > 0.8216, PPE (probabilities of paternity exculation, PPE) = or > 0.4903. The result indicated that there was a significant difference between Dongxiang ethnic group and the white and the black. There was no significant difference in Han nationality. These result filled the Dongxiang ethnic group-a specific group of Chinese into the genetic database and played an important role in Chinese population genetic study and forensic medicine application.
View Article and Find Full Text PDF

Download full-text PDF

Source
May 2002

[Polymorphism of nine STR locus in Nu population from Yunnan Province].

Yi Chuan 2002 Mar;24(2):125-30

China People's Public Security University, Beijing 100038, China.

In this study,blood samples were randomly drawn from 84 unrelated Nu individuals. The polymorphism of nine STR loci and Amelogenin locus were determined by DNA GeneScan. The genetic database on the distribution of gene frequency on the nine STR loci was established, statistical results showed that the genotype distributions were in agreement with Hardy-Weinberg equation. Compared with other population,the results in our study were of great value in human DNA genetic data instant method with the characteristics of precision and sensitivity.
View Article and Find Full Text PDF

Download full-text PDF

Source
March 2002

[Analysis on HLA haplotypes of loci HLA-A, -B, and -DRB1 in northwest Chinese Han population].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2005 Aug;22(4):464-6

The Key Laboratory of National Ministry of Health for Forensic Sciences, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 PR China.

Objective: To investigate HLA-A, -B and -DRB1 allele and HLA-A-B, B-DRB1, A-B-DRB1 haplotype frequencies in the northwest Chinese Han population.

Methods: The authors investigated the HLA-A, -B, -DRB1 allele and haplotype in a northwest Chinese Han population based on 62 families and 101 individuals by use of PCR-sequence specific oligonucleotide probes(PCR-SSOP) DNA typing methods.

Results: Fifteen alleles for the locus HLA-A, 28 alleles for the HLA-B locus and 13 alleles for the HLA-DRB1 were detected. The results showed that the most frequent HLA alleles found were A02 (0.3244), B13 (0.1200), and DRB1*15 (0.1400). Allele frequencies of more than 10% for HLA antigens were A02, A11, A24, B13, B15, B40, DRB1*04, DRB1*07, DRB1*09, DRB1*15. In the analysis of HLA haplotypes, 122 kinds of HLA-A-B haplotypes and 147 kinds HLA-B-DRB1 haplotypes were found. Two hundred and seventy-eight kinds of HLA-A-B-DRB1 haplotypes were found, comprising 61.78%(278/450) of total theoretical haplotypes. Eighty-three kinds of HLA-A-B-DRB1 haplotypes were shown to have at least two same haplotypes, comprising 18.44%(83/450) of total theoretical haplotypes. The most common HLA-A-B-DRB1 haplotypes were A30-B13-DRB1*07, A02-B46-DRB1*09, A01-B37-DRB1*10, A24-B15-DRB1*15, A02-B46-DRB1*08, A33-B58-DRB1*03.

Conclusion: The data can be used for the estimation of the probability of finding haplotypically identical, related or unrelated bone marrow donor for an individual patient, and forensic and paternity tests to estimate the frequency of a DNA profile or anthropologic research.
View Article and Find Full Text PDF

Download full-text PDF

Source
August 2005

[Genetic polymorphism of 9 STR loci in Chaoxian National Minority of China].

Yi Chuan 2002 Nov;24(6):636-8

Forensic Laboratory of Ministry of Health of Xi'an Jiaotong University,710061, Xi'an China.

In order to enrich the Chinese genetic database,nine polymorphic loci of STR,such as D3S1358,vWA,FGA,TH01,TPOX,CSF1PO,D5S818,D13S317 and D7S820 were studied. Based on STR gene scan marked by fluorescence,91 unrelated Chinese Chaoxian individuals were observed.81 alleles and 196 genotypes were found. The corresponding gene frequency and genotype frequency were 0.0055-0.4615 and 0.0110-0.9890 respectively. The genogypes frequency of nine STR loci was good with the Hardy-Weinberg equilibrium (P approximately 0.05). The statistical analysis of nine STR loci showed the following: PIC (polymorphic information content) >or=0.6863, H (heterozygosity) >or=0.6919, DP (discrimination power) >or=0.8301, EPP (probability of paternity exclusion) >or=0.8590. The data studied can be used in Chinese population genetic studies and forensic medicine applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
November 2002

[The study on HLA-Cw polymorphism from Xi'an Han population by PCR-sequence specific oligonucleotide probe].

Fa Yi Xue Za Zhi 2004 ;20(4):197-9

The National Laboratory of the Ministry of Health for Forensic Sciences, Xi'an Jiaotong University, Xi'an 710061, China.

Objective: To determine the HLA-Cw allele and genotype frequencies from Xi'an Han population and obtain genetic data.

Methods: The results of HLA-Cw typing for 130 randomly selected from Xi'an Han population were obtained by using the PCR-Sequence Specific Oligonucleotide Probes (SSOP).

Results: In this investigation, 16 alleles were detected among 130 unrelated individuals with frequencies from 0.0077 to 0.1588 and HLA-Cw*01,03,07 were the most common HLA-Cw alleles. We have made a survey of HLA-Cw alleles frequencies in Xi'an Han Population, with blank frequency being lowered to 0.018 2.

Conclusion: The distribution of genotype frequencies met the law of Hardy-Weinberg equilibrium by Hi-square test. The frequency data can be used in forensic and paternity tests, transplant matching and anthropology.
View Article and Find Full Text PDF

Download full-text PDF

Source
April 2006

[The study of HLA-Cw polymorphism in Uygur population].

Yi Chuan 2003 Sep;25(5):549-51

National Laboratory of Forensic Sciences, Xi'an Jiaotong University, Xi'an 710061, China.

The HLA-Cw loci polymorphism in Uygur population was investigated using the PCR- sequence specific oligonucleotide probe (SSOP) method,and the genetic database on the distribution of gene frequency of the HLA-Cw loci was established. From 146 individuals of Uygur population,18 HLA-Cw alleles were detected. The gene frequency was from 0.0069 to 0.2460. The four most common alleles were HLA-Cw*04(24.60%),07(11.51%),08(10 10%),14(12.02%),and they covered 58.23% of total alleles detected from Uygur population.We have made a survey of HLA-Cw alleles frequencies in a Uygur population,with blank frequency being lowered to 0.0064. The distribution of genotype frequencies met the law of Hardy-Weinberg equilibrium by hi-square test. The frequency data can be used in forensic and paternity tests to estimate the frequency of a DNA profile in the Uygur population,transplant matching and anthropology.
View Article and Find Full Text PDF

Download full-text PDF

Source
September 2003

[Genetic polymorphism of 9 STR loci in Zhuang national minority of China].

Yi Chuan Xue Bao 2002 Dec;29(12):1052-6

Forensic Laboratory of Ministry of Health of Xi'an Jiaotong University 710061, China.

Genetic distribution for nine STR loci were determined in a Chinese Guangxi Zhuang national minority group based on STR gene scan marked by fluorescence. Sixty-two alleles and 169 genotypes were observed in 91 unrelated Zhuang individuals. The corresponding gene frequency and genotype frequency were 0.0054-0.5495 and 0.0110-0.3297 respectively. The expected and observed genotype frequency of nine STR loci was in accordance with the Hardy-Weinberg equilibrium (P > 0.05). The statistical analysis of nine STR loci showed PIC (polymorphic information content) > or = 0.6088, H (heterozygosity) > or = 0.6174, DP (discrimination power) > or = 0.8028, PPE (probability of paternity exclusion) > or = 0.8165. The genetic distance figured with 9 STR genetic data showed that there were significant differences between Chinese Zhuang national minority and the American White and the American Black, and there was little difference between Zhuang national minority and the Chinese Xi'an Han. The result of clusting showed that the present data were divided into three groups: the Black, the White and the Yellow (the Chinese).
View Article and Find Full Text PDF

Download full-text PDF

Source
December 2002

[Studies of STR polymorphisms in Pumi and Lisu minorities in China].

Yi Chuan Xue Bao 2002 ;29(11):959-65

Department of Forensic Science, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.

In this study, we investigated the polymorphisms of STR of Pumi and Lisu minorities with STR genescan marked by fluorescence. Eighty-five alleles of 9 STR in Pumi were detected with the frequency 0.0050-0.5250 and 194 genotypes were found with frequency of 0.0098-0.3235. Sixty three alleles with their frequency of 0.0050-0.4802 and 145 genotypes were found out with frequency of 0.0099-0.3664 in Lisu population. Hi-Square test indicated the distribution of genotypes agreed with Hardy-Weinberg equilibrium (P > 0.05). Statistical analysis showed the followings: H > 0.6 in each locus, the average PIC > 0.7, mean DP > 0.8, EPP > 0.5, indicating the STR markers used in the study were of great value in the researches on minority genetics.
View Article and Find Full Text PDF

Download full-text PDF

Source
July 2003

[STR polymorphisms in four Xinjiang ethnic groups in China].

Yi Chuan Xue Bao 2002 Sep;29(9):761-7

National Laboratory of Forensic Sciences, Xi'an Jiaotong University, Xi'an 710061, China.

Population genetic studies were performed in Xinjiang's Uygur, Sibe, Ozbek and Kirgiz. Allele frequency distributions were analyzed for ten loci, i.e., D3S1358, VWA, CSF1PO, FGA, THO1, TPOX, D5S818, D13S317 and D7S820 by GeneScan. The results showed that there were 66 STR alleles and 148 genotypes in Uygur; 72 STR alleles and 163 genotypes in Sibe; 65 STR alleles and 168 genotypes in Ozbek; 71 STR alleles and 191 genotypes in Kirgiz. Significant differences were identified among ethnic groups (African-American, US-Caucasian and Chinese-Oriental), but similarity was found among the four Xinjiang population. These findings indicated that the nine STR loci and Amelogenin locus were very useful for individual identification in forensic science. It is further certified that Chinese nationality is a whole.
View Article and Find Full Text PDF

Download full-text PDF

Source
September 2002

[Genetic structure of STR in Naci ethnic group in China].

Yi Chuan Xue Bao 2002 Jul;29(7):576-80

Department of Forensic Science, Medical College, Xi'an Jiaotong University, Xi'an 710061, China.

STR is a universal genetic marker that has changeable polymorphism and stable heredity in human genome. It is a specific DNA segment composed of 2-7 base pairs as its core sequence, and is formed through the repeated connection of the same one. Since it has the characteristics such as numerous allelic genes, highly heterozygosity and easy recognition and short PCR segment, it is employed as an ideal DNA marker in such practical fields as human genetics and forensic medicine. In this study, we investigated the polymorphism of STR of Naci minority with STR genescan marked by fluorescence. Seventy-two alleles of 9 STR in Naci were detected with their frequency 0.0052-0.5208 and 165 genotypes were found out with frequency 0.0104-0.3021. Hi-Square test indicated the distribution of genotypes agreed with Hardy-Weinberg equilibrium (P > 0.05). Statistical analysis showed the followings: the heterozygosity (H) > 0.6 in each locus, the average polymorphism information content (PIC) > 0.7, Mean discrimination power (DP) > 0.8, probability of paternity exclusion (EPP) > 0.5, indicating that the STR markers used in the study were of great value in the researches of minority genetics. This not only founds the base for genetic structures of STR of Chinese but also provides valuable information for anthropology, forensic medicine and ethnology.
View Article and Find Full Text PDF

Download full-text PDF

Source
July 2002

Analysis of DNA polymorphism at HLA-A locus by PCR amplification with sequence specific oligonucleotide probe in Chinese Han and Uygur populations.

Yi Chuan Xue Bao 2002 May;29(5):384-9

National Laboratory of Forensic Sciences, Xi'an Jiaotong University, Xi'an 710061, China.

To determine HLA-A genetic polymorphism in Chinese populations and establish ethnic genetic database, 165 Han and 162 Uygur subjects were investigated with a non-isotopic and sensitive method PCR-SSOP. 22 alleles were identified in Han with the most frequent allele being HLA-A * 1101 (19.7%), followed by * 0201 (12.72%). Also, 22 alleles were identified in Uygur with * 2407 (17.90%) being the most frequent one and the frequencies of following alleles: * 0201, * 0101, * 3301 were higher than 10%. HLA-A * 0203, * 0205, * 0210, * 0302, * 2403 and * 3302 were only detected in Han; meanwhile * 0205, * 0211, * 2301, * 2502, * 68012 and * 6802 were only in Uygur. According to Hardy-Weinberg equilibrium, each allele showed no significant (P > 0.05) deviation between the expected frequency and the observed one. Heterozygosity (H), discrimination power (DP) and probability of paternity exclusion (EPP) of HLA-A locus from Han nationality were computed to be 0.9029, 0.9776 and 0.8592; and those from Uygur as 0.9063, 0.9379 and 0.7885. These results suggest that HLA-A DNA polymorphism and the database of two Chinese populations have useful applications in processing forensic casework (as personal identification, paternity test), tracing population migration and genetic diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
May 2002