Publications by authors named "Jianfeng Sun"

84 Publications

Effects of organic acids on color intensification, thermodynamics, and copigmentation interactions with anthocyanins.

Food Chem 2022 Jul 14;396:133691. Epub 2022 Jul 14.

College of Food Science and Technology, Hebei Agricultural University, 289th Lingyusi Street, Lianchi District, Baoding 071000, China.

Anthocyanins are attractive alternatives to synthetic colorants, but their low stability impedes practical applications. Intermolecular copigmentation can enhance both color intensity and stability. Herein, the copigmentation interactions of Kyoho grape skin anthocyanins (KSA) or cyanidin-3-O-glucoside (Cy-G) with organic acids were investigated. Color enhancement was evaluated at different acid molar ratios and treatment temperatures. The optimal copigmentation effects were observed for KSA/tannic acid (1:150) and Cy-G/tannic acid (1:100). Based on enthalpy variation, KSA/ferulic acid and Cy-G/ferulic acid exhibited the highest stability. The distinct color differences observed in the presence of different acids were attributed to structural effects. The influence of ferulic acid on various anthocyanins was also evaluated using theoretical approaches. Owing to steric hindrance, the acyl groups in KSA affected the spatial conformation, hydrogen bonding, and van der Waals interactions of the complexes. Further, hydroxyl groups decreased complex stability. These findings contribute to furthering the understanding of copigmentation effects.
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http://dx.doi.org/10.1016/j.foodchem.2022.133691DOI Listing
July 2022

All-fiber low-frequency shifter based on acousto-optic interaction and its heterodyne vibration response.

Opt Lett 2022 Jul;47(14):3419-3422

We demonstrate two all-fiber low-frequency shift schemes based on the acousto-optic interaction in a few-mode fiber (FMF). Two acoustically induced fiber gratings (AIFGs) are cascaded in reverse to achieve an efficient cycle conversion between LP and LP core modes in the FMF while obtaining a frequency shift of 1.8 MHz. In addition, a long-period fiber grating (LPFG) is employed to replace the AIFG, which achieves a lower frequency shift of 0.9 MHz, and its tunable wavelength range exceeds 100 nm. Both schemes show the characteristics of an upward frequency shift. Moreover, we also present a heterodyne detection system based on the above frequency shift schemes, which is verified in response to micro-vibration signals ranging from tens to hundreds of kilohertz, as well as speech signals in a lower frequency range. The experimental results show that these all-fiber frequency shift schemes have potential applications, such as in fiber optic hydrophones, laser speech detection, and fiber optic sensors.
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http://dx.doi.org/10.1364/OL.463739DOI Listing
July 2022

Role of magnesium-doped calcium sulfate and β-tricalcium phosphate composite ceramics in macrophage polarization and osteo-induction.

Odontology 2022 Jun 2. Epub 2022 Jun 2.

The Affiliated Stomatology Hospital of Kunming Medical University, No. 1088 Haiyuan Middle Road, Kunming, 650500, Yunnan, China.

In the current study, we explored the role of Mg-doped CaSO/β-TCP composite biopolymer in regulating macrophage polarization and its relation with enhanced osteogenic differentiation of periodontal ligament stem cells. Furthermore, mechanism underling the regulation of macrophage polarization by CaSO/β-TCP was evaluated. Mg-doped CaSO/β-TCP composite was characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). Macrophage polarization was characterized using flow cytometry analysis. Macrophage morphometric analysis was conducted by FITC phalloidin staining. Western blot and qRT-PCR assays were used to assess gene expression levels and miRNAs, respectively. SEM morphology of CaSO/β-TCP ceramic revealed a particle size of 10-50 μm, and XRD spectrum showed that characteristic peak of samples was consistent with that of CaSO and β-TCP. Results from flow cytometry evidenced significant upregulation of M2 macrophage markers after adding ceramic biopolymer, indicating the induction of inactivated M0 macrophage polarization to M2 macrophage. Macrophage morphometric analysis revealed development of lamellar pseudopodia on day 7 in CaSO/β-TCP group. Furthermore, flow cytometry revealed high positivity rate of 90.34% (CD44) and 89.36% (CD146). qRT-PCR results showed that the level of miR-21-5p was significantly decreased in M2 macrophages. Moreover, western blot analysis revealed upregulated expression levels of RUNX2, osterix (Osx), and osteopontin (OPN), and ELISA exhibited increase in cytokine levels (IL-1β, IL-10, TGF-β1, and BMP-2) in the presence of macrophages, indicating the osteogenic differentiation ability of periodontal ligament stem cells. The study evidenced the regulation of macrophage polarization by Mg-doped CaSO/β-TCP composite ceramic and its mediation through lncRNA PVT1/miR-21-5p/smad2 molecular axis.
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http://dx.doi.org/10.1007/s10266-022-00708-6DOI Listing
June 2022

Oral antimicrobial peptide-EGCG nanomedicines for synergistic treatment of ulcerative colitis.

J Control Release 2022 07 25;347:544-560. Epub 2022 May 25.

State Key Laboratory of Silkworm Genome Biology, College of Sericulture, Textile, and Biomass Sciences, Southwest University, Beibei, Chongqing 400715, China; Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Beibei, Chongqing 400715, China. Electronic address:

The pathogenesis of ulcerative colitis (UC) is associated with severe inflammation, damaged colonic barriers, increased oxidative stress, and intestinal dysbiosis. The majority of current medications strive to alleviate inflammation but fail to target additional disease pathologies. Addressing multiple symptoms using a single 'magic bullet' remains a challenge. To overcome this, a smart epigallocatechin-3-gallate (EGCG)-loaded silk fibroin-based nanoparticle (NP) with the surface functionalization of antimicrobial peptides (Cathelicidin-BF, CBF) was constructed, which could be internalized by Colon-26 cells and RAW 264.7 macrophages with high efficiencies. The resulting CBF-EGCG-NPs efficiently restored colonic epithelial barriers by relieving oxidative stress and promoting epithelium migration. They also alleviated immune responses through downregulation of pro-inflammatory factors, upregulation of anti-inflammatory factors, M2 macrophage polarization, and lipopolysaccharide (LPS) elimination. Interestingly, oral administration of hydrogel (chitosan/alginate)-embedding CBF-EGCG-NPs could not only retard progression and treat UC, but also modulate intestinal microbiota by increasing their overall diversity and richness and augmenting the abundance of beneficial bacteria (e.g., Firmicutes and Lactobacillaceae). Our work provides a "many birds with one stone" strategy for addressing UC symptoms using a single NP-based oral platform that targets immune microenvironment modulation, LPS clearance, and microbial remodeling.
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http://dx.doi.org/10.1016/j.jconrel.2022.05.025DOI Listing
July 2022

Near-Infrared-Enpowered Nanomotor-Mediated Targeted Chemotherapy and Mitochondrial Phototherapy to Boost Systematic Antitumor Immunity.

Adv Healthc Mater 2022 07 18;11(14):e2200255. Epub 2022 May 18.

State Key Laboratory of Silkworm Genome Biology, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing, 400715, China.

Phototherapy is an important strategy to inhibit tumor growth and activate antitumor immunity. However, the effect of photothermal/photodynamic therapy (PTT/PDT) is restricted by limited tumor penetration depth and unsatisfactory potentiation of antitumor immunity. Here, a near-infrared (NIR)-driven nanomotor is constructed with a mesoporous silicon nanoparticle (MSN) as the core, end-capped with Antheraea pernyi silk fibroin (ApSF) comprising arginine-glycine-aspartate (RGD) tripeptides. Upon NIR irradiation, the resulting ApSF-coated MSNs (DIMs) loading with photosensitizers (ICG derivatives, IDs) and chemotherapeutic drugs (doxorubicin, Dox) can efficiently penetrate into the internal tumor tissues and achieve effective phototherapy. Combined with chemotherapy, a triple-modal treatment (PTT, PDT, and chemotherapy) approach is developed to induce the immunogenic cell death of tumor cells and to accelerate the release of damage-associated molecular patterns. In vivo results suggest that DIMs can promote the maturation of dendritic cells and surge the number of infiltrated immune cells. Meanwhile, DIMs can polarize macrophages from M2 to M1 phenotypes and reduce the percentages of immunosuppressive Tregs, which reverse the immunosuppressive tumor microenvironment and activate systemic antitumor immunity. By achieving synergistic effects on the tumor inhibition and the antitumor immunity activation, DIMs show great promise as new nanoplatforms to treat metastatic breast cancer.
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http://dx.doi.org/10.1002/adhm.202200255DOI Listing
July 2022

PSRR: A Web Server for Predicting the Regulation of miRNAs Expression by Small Molecules.

Front Mol Biosci 2022 21;9:817294. Epub 2022 Mar 21.

University Hospital for Gynecology, Pius-Hospital, University Medicine Oldenburg, Oldenburg, Germany.

MicroRNAs (miRNAs) play key roles in a variety of pathological processes by interacting with their specific target mRNAs for translation repression and may function as oncogenes (oncomiRs) or tumor suppressors (TSmiRs). Therefore, a web server that could predict the regulation relations between miRNAs and small molecules is expected to achieve implications for identifying potential therapeutic targets for anti-tumor drug development. Upon obtaining positive/known small molecule-miRNA regulation pairs from SM2miR, we generated a multitude of high-quality negative/unknown pairs by leveraging similarities between the small molecule structures. Using the pool of the positive and negative pairs, we created the Dataset1 and Dataset2 datasets specific to up-regulation and down-regulation pairs, respectively. Manifold machine learning algorithms were then employed to construct models of predicting up-regulation and down-regulation pairs on the training portion of pairs in Dataset1 and Dataset2, respectively. Prediction abilities of the resulting models were further examined by discovering potential small molecules to regulate oncogenic miRNAs identified from miRNA sequencing data of endometrial carcinoma samples. The random forest algorithm outperformed four machine-learning algorithms by achieving the highest AUC values of 0.911 for the up-regulation model and 0.896 for the down-regulation model on the testing datasets. Moreover, the down-regulation and up-regulation models yielded the accuracy values of 0.91 and 0.90 on independent validation pairs, respectively. In a case study, our model showed highly-reliable results by confirming all top 10 predicted regulation pairs as experimentally validated pairs. Finally, our predicted binding affinities of oncogenic miRNAs and small molecules bore a close resemblance to the lowest binding energy profiles using molecular docking. Predictions of the final model are freely accessible through the PSRR web server at https://rnadrug.shinyapps.io/PSRR/. Our study provides a novel web server that could effectively predict the regulation of miRNAs expression by small molecules.
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http://dx.doi.org/10.3389/fmolb.2022.817294DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8979021PMC
March 2022

Improving TVB-N prediction in pork using portable spectroscopy with just-in-time learning model updating method.

Meat Sci 2022 Jun 14;188:108801. Epub 2022 Mar 14.

College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China. Electronic address:

Near infrared spectroscopy (NIR) technology is an effective method for nondestructive prediction of total volatile basic nitrogen (TVB-N) in pork. However, the NIR models lack robustness and often fail when used on a new batch. To handle the problem and obtain better prediction performance, a model updating method based on just-in-time learning (JITL) was proposed in this study. A comprehensive similarity criterion considering both input (spectra) and output (TVB-N content) information was designed. Combining a defined similarity factor, the most relevant samples to new batch samples were selected and a local least square support vector machine model was established in real time based on the selected samples. The results showed that the models updated with JITL approach kept a high predictive performance on new independent batch with prediction error decreasing from 2.95 to 1.60 mg/100 g. The robust models made on selected similar samples combined with JITL model updating strategy can support to make NIR spectroscopy a preferred choice for non-destructive assessment of quality features in pork meat.
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http://dx.doi.org/10.1016/j.meatsci.2022.108801DOI Listing
June 2022

CEP128 is involved in spermatogenesis in humans and mice.

Nat Commun 2022 03 16;13(1):1395. Epub 2022 Mar 16.

Department of Obstetrics/Gynecology, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, 610041, China.

Centrosomal proteins are necessary components of the centrosome, a conserved eukaryotic organelle essential to the reproductive process. However, few centrosomal proteins have been genetically linked to fertility. Herein we identify a homozygous missense variant of CEP128 (c.665 G > A [p.R222Q]) in two infertile males. Remarkably, male homozygous knock-in mice harboring the orthologous CEP128 variant show anomalies in sperm morphology, count, and motility. Moreover, Cep128 knock-out mice manifest male infertility associated with disrupted sperm quality. We observe defective sperm flagella in both homozygous Cep128 KO and KI mice; the cilia development in other organs is normal-suggesting that CEP128 variants predominantly affected the ciliogenesis in the testes. Mechanistically, CEP128 is involved in male reproduction via regulating the expression of genes and/or the phosphorylation of TGF-β/BMP-signalling members during spermatogenesis. Altogether, our findings unveil a crucial role for CEP128 in male fertility and provide important insights into the functions of centrosomal proteins in reproductive biology.
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http://dx.doi.org/10.1038/s41467-022-29109-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8927350PMC
March 2022

Edible plant-derived nanotherapeutics and nanocarriers: recent progress and future directions.

Expert Opin Drug Deliv 2022 04 25;19(4):409-419. Epub 2022 Mar 25.

State Key Laboratory of Silkworm Genome Biology, College of Sericulture, Textile, and Biomass Sciences, Southwest University, Chongqing, Sichuan, China.

Introduction: High drug delivery efficiency, desirable therapeutic effects, and low toxicity have become crucial to develop nanotherapeutics. Natural nanoparticles (NPs) from edible plants contain a large quantity of bioactive small molecules, proteins, glycolipids, and microRNAs. The development of these NPs has rapidly attracted increasing attention due to their merits of green production, excellent biocompatibility, anti-inflammatory activities, and antitumor capacities.

Areas Covered: Here, we introduce the extraction, purification, and construction strategies of plant-derived exosome-like NPs (PDENs) and expound on their physicochemical properties, biomedical functions, and therapeutic effects against various diseases. We also recapitulate future directions and challenges of the emerging nanotherapeutics.

Expert Opinion: PDENs have been used as natural nanotherapeutics and nanocarriers. The challenges of applying PDENs primarily stem from the lack of understanding of the mechanisms that drive the tissue-specific targeting properties. Elucidating the underlying targeting mechanisms is one of the major focuses in this review, which helps to gain new research opportunities for the development of natural nanotherapeutics. Despite excellent biosafety and therapeutic effects in the treatment of various diseases, the medical translation of these NPs has still been limited by low yields and cold-chain dependence. Therefore, exploiting new techniques will be required for their massive production and storage.
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http://dx.doi.org/10.1080/17425247.2022.2053673DOI Listing
April 2022

Multi-dimensional and large-sized optical phased array for space laser communication.

Opt Express 2022 Feb;30(4):5026-5037

In this study, we propose a novel multi-dimensional and large-sized optical phased array theory for space laser communication that addresses the theoretical limitations of the conventional optical phased array. We theoretically analyzed the principle of this phased array technology. The results of simulation and laboratory experiment clearly showed it can realize the large scanning angle and high optical gain required for communication. The novel optical phased array theory is of great significance to the revolution of miniaturization and networking in the field of space laser communication.
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http://dx.doi.org/10.1364/OE.447351DOI Listing
February 2022

Oral nanotherapeutics based on Antheraea pernyi silk fibroin for synergistic treatment of ulcerative colitis.

Biomaterials 2022 03 16;282:121410. Epub 2022 Feb 16.

State Key Laboratory of Silkworm Genome Biology, College of Sericulture, Textile, and Biomass Sciences, Southwest University, Chongqing, 400715, China. Electronic address:

Ulcerative colitis (UC) with its rapidly increasing incidence has become an emerging challenge for public health. Therapeutic agents are required to be specifically delivered to colon epithelial cells and macrophages with controlled release in the cytoplasm for wound healing, inflammation alleviation, and microbiota rebalance. As a promising biomaterial for accomplishing this, Antheraea pernyi silk fibroin (ApSF) was selected and engineered to form nanoparticles (NPs) loaded with the anti-inflammatory drug, resveratrol (Res). The intrinsic features of these fabricated Res-ApNPs included targeting of colonic epithelial cells and macrophages, lysosomal escape capacity, and responsiveness to pH, reactive oxygen species (ROS), and glutathione, which were pertinent to their functional units such as arginine-glycine-aspartate tripeptides, α-helixes, β-sheets, and disulfide bonds, enabling on-demand release of Res molecules in the cytoplasm of target cells. The Res-ApNP treatment restored damaged colonic epithelial barriers, polarized macrophages to type M2, alleviated inflammatory reactions, and reduced the level of intracellular ROS. Oral treatment with chitosan-alginate hydrogels embedded with Res-ApNPs substantially relieved UC symptoms, as evidenced by decreased colonic inflammation, increased synthesis of tight-junction proteins, and rebalanced intestinal microbiota. Our findings suggest that these high-performance ApSF-based NPs can be developed as effective drug carriers for oral UC treatment.
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http://dx.doi.org/10.1016/j.biomaterials.2022.121410DOI Listing
March 2022

Biodegradation of Nicotine and TSNAs by Bacterium sp. Strain J54.

Iran J Biotechnol 2021 Jul 1;19(3):e2812. Epub 2021 Jul 1.

Yunnan Academy of Tobacco Agricultural Sciences, Kunming, China 650021.

Background: Microorganisms play an important role in reducing harmful substances in flue-cured tobacco. Numerous studies have been conducted to degrade nicotine by microorganisms.

Objectives: The present research deals with the isolation of a potent bacterial strain able to efficiently degrade nicotine and tobacco-specific nitrosamines (TSNAs) in flue-cured tobacco.

Material And Methods: Bacterial strain J54, capable of efficiently degrading nicotine and tobacco-specific nitrosamines (TSNAs), was isolated from tobacco leaves and identified. The strain J54 can use nicotine as the sole carbon and nitrogen source and could effectively degrade nicotine while growing in a nicotine isolation medium (NIM) medium.

Results: Compared with the control (CK), the total TSNAs content in the tobacco flue-cured eaves after being sprayed with a solution of the J54 strain was found to decrease by 26.22%. Therein, the degradation rates of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK), N'-nitrosonornicotine (NNN), N'-nitrosoanatabine (NAT), and N'-nitrosoanabasine (NAB) were 24.01%, 26.27%, 28.6%, and 1.83%, respectively.

Conclusions: Bacterial strain J54, was isolated from tobacco leaves and identified as a bacterium, which is similar to based on its morphological and biochemical characteristics and by phylogenetic analysis based on 16S rRNA gene sequences. To our knowledge, this is the first report of the isolation and characterization of a sp. strain that can efficiently degrade nicotine and TSNAs. The findings pave the way for the application of new biotechnologies for the degradation of nicotine and TSNAs by microorganisms.
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http://dx.doi.org/10.30498/ijb.2021.240460.2812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590725PMC
July 2021

Complex Age- and Cancer-Related Changes in Human Blood Transcriptome-Implications for Pan-Cancer Diagnostics.

Front Genet 2021 15;12:746879. Epub 2021 Oct 15.

School of Medicine, Institute of Genomics, Huaqiao University, Xiamen, China.

Early cancer detection is the key to a positive clinical outcome. While a number of early diagnostics methods exist in clinics today, they tend to be invasive and limited to a few cancer types. Thus, a clear need exists for non-invasive diagnostics methods that can be used to detect the presence of cancer of any type. Liquid biopsy based on analysis of molecular components of peripheral blood has shown significant promise in such pan-cancer diagnostics; however, existing methods based on this approach require improvements, especially in sensitivity of early-stage cancer detection. The improvement would likely require diagnostics assays based on multiple different types of biomarkers and, thus, calls for identification of novel types of cancer-related biomarkers that can be used in liquid biopsy. Whole-blood transcriptome, especially its non-coding component, represents an obvious yet under-explored biomarker for pan-cancer detection. In this study, we show that whole transcriptome analysis using RNA-seq could indeed serve as a viable biomarker for pan-cancer detection. Furthermore, a class of long non-coding (lnc) RNAs, very long intergenic non-coding (vlinc) RNAs, demonstrated superior performance compared with protein-coding mRNAs. Finally, we show that age and presence of non-blood cancers change transcriptome in similar, yet not identical, directions and explore implications of this observation for pan-cancer diagnostics.
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http://dx.doi.org/10.3389/fgene.2021.746879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8554330PMC
October 2021

Comparative Effectiveness of Minimally Invasive Nonsurgical Treatments for Plantar Fasciitis: A Network Meta-analysis of 30 Randomized Controlled Trials.

Pain Physician 2021 11;24(7):E955-E971

Dept. of Orthopaedic and Trauma Surgery, Xiyuan Hospital, China Academy of Chinese Medical Science, Beijing, China.

Background: Several minimally invasive nonsurgical treatments have been widely applied for plantar fasciitis (PF). To date, controversy still exists regarding the effectiveness of these approaches for treating PF.

Objective: The purpose of this study was to perform a comprehensive comparison of the currently available invasive nonsurgical treatments for PF regarding short- and mid-term reductions in pain using a network meta-analysis (NMA).

Study Design: NMA of randomized controlled trials (RCTs) for minimally invasive nonsurgical treatments of PF.

Methods: The EMBASE, PubMed, and Cochrane Central Register of Controlled Trials (CENTRAL) databases were searched for eligible studies. Patients were adults age >= 18 years with PF. The outcome measures were the visual analog scale (VAS) scores at 3-6 weeks and 4-6 months. Pairwise meta-analysis and NMA based on a Bayesian analysis were performed, and all potential comparisons and rank of probabilities were calculated.

Results: Thirty RCTs were included in the NMA. The trials investigated 20 treatments or combined treatments, including autologous whole blood, botulinum toxin A (BTA), ultrasound-guided gastrocnemius injection of botulinum toxin (BTA in the gastrocnemius), corticosteroid (CS), miniscalpel-needle (MSN), placebo, platelet-rich plasma (PRP), and the ultrasound-guided technique and peppering technique (PEP). The MSN treatment may be the best choice.

Limitations: Some treatments were investigated in only one study or at one follow-up period and were separated from the network at 4-6 months. Other limitations include the inconformity of the treatment schedule and dose.

Conclusions: The MSN treatment should be recommended as the best therapy, followed by BTA in the gastrocnemius and BTA. CS and PRP are common medications that remain valuable in clinical practice. PEP can be performed after the injection of medication.
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November 2021

Inter-satellite laser-ranging based on intradyne coherent detection.

Appl Opt 2021 Oct;60(28):8930-8938

With the development of laser communication networking, laser-ranging technology is becoming more and more applicable. In this paper, high-accuracy ranging is implemented based on intradyne coherent detection at a communication rate of 1048.576 Mbps. The ranging accuracy is affected by clock phase calculation error and code loop track error. Parallel clock phase difference calculation, frame head correlation, and ranging ambiguity region handle are combined with the ranging calibration method, realizing millimeter-level corrected distance measurement. Dynamic range measurement above 1 m is proven to be continuous through the ranging ambiguity region handle. In addition, high-precision clock frequency deviation between two asynchronous terminals can be obtained through derivation of one-way distance at static ranging or by derivative of distance difference at bidirectional ranging. The methods proposed in this paper are verified by inter-satellite laser-ranging on orbit, and the results are analyzed.
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http://dx.doi.org/10.1364/AO.434807DOI Listing
October 2021

Physician-modified Endograft with Left Subclavian Artery Fenestration for Ruptured Type B Aortic Dissection.

Ann Vasc Surg 2021 Nov 26;77:352.e7-352.e11. Epub 2021 Aug 26.

Department of Vascular Surgery, Yichang Central People's Hospital, The First College of Clinical Medical Science, China Three Gorges University, Hubei, China.

A 56-year-old male patient was transferred to our institution with acute chest and back pain and deteriorating vital signs for 3 days. Emergent computed tomography angiography (CTA) revealed ruptured type B aortic dissection with large left hemothorax. The dissection extended into the left subclavian artery (LSA). Immediate endovascular aortic repair with LSA coverage to extend the proximal landing zone was planned. Fenestrated thoracic endovascular repair (fTEVAR) was performed using a physician-modified endograft (PMEG) to maintain LSA perfusion. The thoracic endograft was modified on a back table while anesthesia was given, and arterial accesses were acquired. FTEVAR was performed smoothly without any complication. Completion angiogram showed no evidence of endoleak or active bleeding. Chest tube was then placed, and the left lung gradually expanded. Postoperative hospital courses were uneventful. Follow-up CTA showed the thoracic endograft and the LSA stent were in good position, and the rupture thoracic aorta was completely sealed. Chest tube was removed on postoperative day (POD) 7. He was discharged home on POD 20 without any complications. Detailed techniques of PMEG for LSA fenestration are described.
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http://dx.doi.org/10.1016/j.avsg.2021.05.060DOI Listing
November 2021

Can Kinesio taping improve discomfort after mandibular third molar surgery? A systematic review and meta-analysis.

Clin Oral Investig 2021 Sep 23;25(9):5139-5148. Epub 2021 Jul 23.

Department of Orthodontics, Ningbo Stomatology Hospital, 315000, Ningbo, China.

Objective: The purpose of this study was to evaluate whether Kinesio taping (KT) can improve patient discomfort after mandibular third molar surgery.

Materials And Methods: This systematic review and meta-analysis was conducted according to the PICO strategy. We searched 4 databases for related articles. All controlled trials or randomized controlled trials that evaluated the application of KT after mandibular third molar surgery were included. Screening and article selection were carried out by two independent reviewers. The main evaluation indicators were facial swelling, pain, and trismus. All statistical analyses were conducted using Review Manager 5.3 software.

Result: This analysis included 8 articles. The combined results showed that compared with the control group, the postoperative application of KT significantly reduced pain in the early (early stage mean difference (MD), - 2.00; 95% confidence interval (CI), - 2.40 to - 1.60; P < 0.00001) and late (late stage MD, - 1.18; 95% CI, - 2.26 to - 0.11; P = 0.03) postoperative periods and, thus, reduced the intake of painkillers. KT also reduced facial swelling in the early and late postoperative periods (early stage standardized mean difference (SMD), - 1.34; 95% CI, - 1.99 to - 0.68; P < 0.0001; late stage SMD, - 0.31; 95% CI, - 0.51 to - 0.11; P = 0.002). In addition, the postoperative application of KT improved restricted mouth opening in the early and late postoperative periods (early stage MD, - 5.03 mm; 95% CI, - 6.32 to - 3.74 mm; P < 0.00001; late stage MD, - 3.42 mm; 95% CI, - 5.31 to - 1.52 mm; P = 0.0004).

Conclusion: KT can significantly reduce postoperative pain, swelling, and trismus after impacted mandibular tooth extraction. Additional high-quality and rigorously designed randomized controlled trials should be conducted to verify these conclusions.

Clinical Relevance: KT is a low-cost, simple, effective nondrug therapy for the postoperative management of mandibular third molar extraction and has broad prospects for clinical application.
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http://dx.doi.org/10.1007/s00784-021-04069-2DOI Listing
September 2021

A Mobile and Self-Powered Micro-Flow Pump Based on Triboelectricity Driven Electroosmosis.

Adv Mater 2021 Aug 16;33(34):e2102765. Epub 2021 Jul 16.

Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, P. R. China.

Electroosmotic pumps have been widely used in microfluidic systems. However, traditional high-voltage (HV)-sources are bulky in size and induce numerous accessional reactions, which largely reduce the system's portability and efficiency. Herein, a motion-controlled, highly efficient micro-flow pump based on triboelectricity driven electroosmosis is reported. Utilizing the triboelectric nanogenerator (TENG), a strong electric field can be formed between two electrodes in the microfluidic channel with an electric double layer, thus driving the controllable electroosmotic flow by biomechanical movements. The performance and operation mechanism of this triboelectric electroosmotic pump (TEOP) is systematically studied and analyzed using a basic free-standing mode TENG. The TEOP produces ≈600 nL min micro-flow with a Joule heat down to 1.76 J cm nL compared with ≈50 nL min and 8.12 J cm nL for an HV-source. The advantages of economy, efficiency, portability, and safety render the TEOP a more conducive option to achieve wider applications in motion-activated micro/nanofluidic transportation and manipulation.
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http://dx.doi.org/10.1002/adma.202102765DOI Listing
August 2021

Missense Variant of Endoplasmic Reticulum Region of Gene Causes Autosomal Dominant Hearing Loss without Syndromic Phenotype.

Biomed Res Int 2021 4;2021:6624744. Epub 2021 Mar 4.

Precision Medicine Center, Academy of Medical Science, Zhengzhou University, Daxuebei Road No. 40, Zhengzhou 450052, China.

Objective: Genetic variants in the gene can cause Wolfram syndrome (WS) or autosomal dominant nonsyndromic low-frequency hearing loss (HL). This study is aimed at investigating the molecular basis of HL in an affected Chinese family and the genotype-phenotype correlation of variants.

Methods: The clinical phenotype of the five-generation Chinese family was characterized using audiological examinations and pedigree analysis. Target exome sequencing of 129 known deafness genes and bioinformatics analysis were performed among six patients and four normal subjects to screen suspected pathogenic variants. We built a complete WFS1 protein model to assess the potential effects of the variant on protein structure.

Results: A novel heterozygous pathogenic variant NM_006005.3 c.2020G>T (p.Gly674Trp) was identified in the gene, located in the C-terminal domain of the wolframin protein. We further showed that HL-related missense variants were mainly concentrated in the endoplasmic reticulum (ER) domain. In contrast, WS-related missense variants are randomly distributed throughout the protein.

Conclusions: In this family, we identified a novel variant p.Gly674Trp of as the primary pathogenic variant causing the low-frequency sensorineural HL, enriching the mutational spectrum of the gene.
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http://dx.doi.org/10.1155/2021/6624744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260318PMC
September 2021

High Pressure Inside Nanometer-Sized Particles Influences the Rate and Products of Chemical Reactions.

Environ Sci Technol 2021 06 1;55(12):7786-7793. Epub 2021 Jun 1.

Univ. Lyon, Université Claude Bernard Lyon 1, CNRS, IRCELYON, Villeurbanne F-69626, France.

The composition of organic aerosol has a pivotal influence on aerosol properties such as toxicity and cloud droplet formation capability, which could affect both climate and air quality. However, a comprehensive and fundamental understanding of the chemical and physical processes that occur in nanometer-sized atmospheric particles remains a challenge that severely limits the quantification and predictive capabilities of aerosol formation pathways. Here, we investigated the effects of a fundamental and hitherto unconsidered physical property of nanoparticles-the Laplace pressure. By studying the reaction of glyoxal with ammonium sulfate, both ubiquitous and important atmospheric constituents, we show that high pressure can significantly affect the chemical processes that occur in atmospheric ultrafine particles (i.e., particles < 100 nm). Using high-resolution mass spectrometry and UV-vis spectroscopy, we demonstrated that the formation of reaction products is strongly (i.e., up to a factor of 2) slowed down under high pressures typical of atmospheric nanoparticles. A size-dependent relative rate constant is determined and numerical simulations illustrate the reduction in the production of the main glyoxal reaction products. These results established that the high pressure inside nanometer-sized aerosols must be considered as a key property that significantly impacts chemical processes that govern atmospheric aerosol growth and evolution.
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http://dx.doi.org/10.1021/acs.est.0c07386DOI Listing
June 2021

Microdroplet extraction assisted ultrasensitive Raman detection in complex oil.

Lab Chip 2021 06;21(11):2217-2222

State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, Chongqing 400044, China.

The Raman detection of trace substances in complex oil is still a great challenge at present because of the strong disturbance of background activity and the suppression of intensity in spectra caused by complicated components. In this work, a simple and robust approach based on microdroplet liquid-liquid extraction for the real-time Raman spectroscopy monitoring of trace substances in complex oil is reported. Based on unbalanced chemical potentials between water and oil on a microfluidic chip, a target trace molecule is extracted from complex mineral oil to a water microdroplet. Benefiting from the real-time fluorescence intensities of fluorescein in a water microdroplet, the extraction performance is investigated and optimized. The optimal water microdroplet is implemented for the Raman detection of furfural in a complex mineral oil, a typical trace performance marker in electric power equipment, and this exhibits excellent sensitivity with a limit of detection (LOD) of 26 ppb. Compared to traditional detection technology for trace substances in complex oil (high performance liquid chromatography, HPLC), this method greatly simplified the process of measurement, reduced the volume of sample required, had a fast measurement time, and exhibited the prospect of real-time monitoring applications with high sensitivity, which not only promotes the development of oil quality but also enlarges existing knowledge related to using Raman spectroscopy in chem-/bio-sensing.
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http://dx.doi.org/10.1039/d1lc00169hDOI Listing
June 2021

Low skeletal muscle mass predicts poor clinical outcomes in patients with abdominal trauma.

Nutrition 2021 09 4;89:111229. Epub 2021 Mar 4.

Department of Intensive Care Unit, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu Province, China. Electronic address:

Objective: The aim of this study was to investigate whether low skeletal muscle mass is associated with clinical outcomes in patients with abdominal trauma.

Methods: Patients presenting to our institution with abdominal trauma from January 2010 to April 2020 were retrospectively included. Low skeletal muscle mass was defined, using computed tomography, as skeletal muscle index (SMI) at the third lumbar vertebra below the lowest sex-specific quartile within 1 wk of admission. Clinical outcomes such as complications, hospital stay, and hospital cost were recorded, and univariate and multivariate analyses were performed.

Results: Among 684 patients, 451 were eligible. Of these, 112 (24.8%) were classified as having low skeletal muscle mass, based on SMI diagnostic cutoff values (42.08 cm/m for men and 37.35 cm/m for women). Low skeletal muscle mass was significantly associated with longer hospital length of stay, longer intensive care length of stay, higher cost, higher frequency of mechanical ventilation, longer duration of vasopressor use, and higher incidence of massive transfusion and overall complications (P < 0.05). Subgroup analysis showed that pneumonia, acute gastrointestinal dysfunction, cholecystitis, digestive tract fistula, gastric fistula, and intestinal fistula were significantly associated with low skeletal muscle mass (P < 0.05). Multivariate analysis showed that low skeletal muscle mass was an independent risk factor for overall complications (odds ratio [OR], 2.44; 95% confidence interval [CI], 1.33-4.49; P = 0.004), hospital length of stay (OR, 3.49; 95% CI, 1.96-6.20; P < 0.001), and hospital cost (OR, 2.67; 95% CI, 1.48-4.80; P = 0.001).

Conclusions: Low skeletal muscle mass could be an independent predictor of poor clinical outcomes in patients with abdominal trauma.
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http://dx.doi.org/10.1016/j.nut.2021.111229DOI Listing
September 2021

Improved sequence-based prediction of interaction sites in α-helical transmembrane proteins by deep learning.

Comput Struct Biotechnol J 2021 9;19:1512-1530. Epub 2021 Mar 9.

Department of Bioinformatics, Wissenschaftzentrum Weihenstephan, Technical University of Munich, Maximus-von-Imhof-Forum 3, 85354 Freising, Germany.

Interactions between transmembrane (TM) proteins are fundamental for a wide spectrum of cellular functions, but precise molecular details of these interactions remain largely unknown due to the scarcity of experimentally determined three-dimensional complex structures. Computational techniques are therefore required for a large-scale annotation of interaction sites in TM proteins. Here, we present a novel deep-learning approach, DeepTMInter, for sequence-based prediction of interaction sites in α-helical TM proteins based on their topological, physiochemical, and evolutionary properties. Using a combination of ultra-deep residual neural networks with a stacked generalization ensemble technique DeepTMInter significantly outperforms existing methods, achieving the AUC/AUCPR values of 0.689/0.598. Across the main functional families of human transmembrane proteins, the percentage of amino acid sites predicted to be involved in interactions typically ranges between 10% and 25%, and up to 30% in ion channels. DeepTMInter is available as a standalone package at https://github.com/2003100127/deeptminter. The training and benchmarking datasets are available at https://data.mendeley.com/datasets/2t8kgwzp35.
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http://dx.doi.org/10.1016/j.csbj.2021.03.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985279PMC
March 2021

Novel mutations in FSIP2 lead to multiple morphological abnormalities of the sperm flagella and poor ICSI prognosis.

Gene 2021 May 23;781:145536. Epub 2021 Feb 23.

Department of Obstetrics/Gynecology, Joint Laboratory of Reproductive Medicine (SCU-CUHK), Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China. Electronic address:

Multiple morphological abnormalities of the sperm flagella (MMAF) is defined as deformities that cause sperm motility disorders, further resulting in male infertility. However, the reported genes related to sperm flagellar defects can only explain approximately 60% of human MMAF cases. Here, we report two novel compound heterozygous mutations, c.16246_16247insCCCAAATATCACC (p. T5416fs*7) and c.17323C > T (p.Q5774*), in the fibrous sheath-interacting protein 2 gene (FSIP2; OMIM: 615796) in an infertile patient by whole-exome sequencing (WES). Western blotting and immunofluorescence staining confirmed that the compound heterozygous mutations abrogated FSIP2 protein expression. Notably, our staining revealed that FSIP2 is expressed in the cytoplasm of primary germ cell and flagella of spermatids during the spermiogenesis. Moreover, intracytoplasmic sperm injection (ICSI) was carried out using sperm from this patient; however, pregnancy failed after embryo transfer through one cycle. Our findings may be helpful in establishing a genetic diagnosis for MMAF, as well as provide additional beneficial knowledge for genetic counseling and infertility treatment.
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http://dx.doi.org/10.1016/j.gene.2021.145536DOI Listing
May 2021

Salvia miltiorrhiza improves type 2 diabetes: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Feb;100(6):e23843

Hospital of Chengdu University of Traditional Chinese Medicine.

Background: Diabetes refers to any group of metabolic diseases characterized by high blood sugar and generally thought to be caused by insufficient production of insulin, impaired response to insulin. Globally, patients with type 2 diabetes account for more than 85% of the total diabetic patients, and due to factors, such as obesity, aging, environment and lifestyle, the incidence of diabetes is rising. Salvia miltiorrhiza (SM) is a medicine used to treat diabetes in China. In recent years, it has been reported that SM has the effect of improving type 2 diabetes. However, there is no systematic review of its efficacy and safety yet. Therefore, we propose a systematic review to evaluate the efficacy and safety of SM for T2D.

Methods: Six databases will be searched: China National Knowledge Infrastructure (CNKI), China Biological Medicine (CBM), China Scientific Journals Database (CSJD), Wanfang database, PubMed, and EMBASE. The information is searched from January 2010 to July 2020. Languages are limited to English and Chinese. The primary outcomes include 2 hour plasma glucose, fasting plasma glucose, hemoglobin A1c, homeostasis model assessment of insulin resistance, and fasting plasma insulin. The secondary outcomes include clinical efficacy and adverse events.

Results: This systematic review will evaluate the efficacy and safety of Salvia miltiorrhiza in the treatment of type 2 diabetes.

Conclusion: This systematic review provides evidence as to whether Salvia miltiorrhiza is effective and safe for type 2 diabetes.

Ethics: Ethical approval is not necessary as this protocol is only for systematic review and does not involve in privacy data or an animal experiment.

Systematic Review Registration: INPLASY2020110046.
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http://dx.doi.org/10.1097/MD.0000000000023843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886461PMC
February 2021

Ginsenosides for the treatment of metabolic syndrome and cardiovascular diseases: Pharmacology and mechanisms.

Biomed Pharmacother 2020 Dec 2;132:110915. Epub 2020 Nov 2.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, PR China. Electronic address:

Epidemiological studies showed that the metabolic syndromes (MetS) and cardiovascular diseases (CVDs) are responsible for a serious threat to human health worldwide. MetS is a syndromes characterized by fat metabolism disorder, obesity, diabetes, insulin resistance and other risk factors, which increases the risk of CVDs initiation and development. Although certain drugs play a role in lowering blood sugar and lipid, some side effects also occur. Considering the multiple pathogenesis, a great deal of natural products have been attempted to treat metabolic syndromes. Ginsenosides, as the active components isolated from Panax ginseng C.A.Mey, have been reported to have therapeutic effects on MetS and CVDs, of which pharmacological mechanisms were further studied as well. This review aims to systematically summarize current pharmacological effects of ginsenosides on MetS and CVDs, potential mechanisms and clinic trials, which will greatly contribute to the development of potential agents for related disease treatment.
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http://dx.doi.org/10.1016/j.biopha.2020.110915DOI Listing
December 2020

Large field of view beaconless laser nutation tracking sensor based on a micro-electro-mechanical system mirror.

Appl Opt 2020 Aug;59(22):6534-6539

We propose a laser nutation tracking sensor for beaconless laser communication, which uses a micro-electro-mechanical system (MEMS) mirror to achieve high-efficiency and large-amplitude nutation at its resonant frequency. We derive a new formula for the case of incompletely detectable optical power in the nutation cycle. In the experiment, we measure the performance of the sensor in calculating boresight error under three different nutation radii. Combining with the proposed algorithm for the new scene, we complete the accurate boresight calculation in the range of ±200µ, at the nutation radius of 4.9 µm. We trust that the receiving field of view (FOV) of this tracking sensor can be further expanded by increasing the nutation radius. The sensor, as proposed in this paper, will be of constructive help to simplify tracking systems in the future.
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http://dx.doi.org/10.1364/AO.396490DOI Listing
August 2020

Development of elastic artificial vessels with a digital pulse flow system to investigate the risk of restenosis and vasospasm.

Lab Chip 2020 08 29;20(16):3051-3059. Epub 2020 Jul 29.

Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials, College of Physics, Chongqing University, Chongqing 401331, China.

The postoperative risk of stenosis is a complex issue, with risk factors including the status of human umbilical vein endothelial cells, the shear stress of dynamic blood flow, and blood physiology. Current research would benefit from in vitro models that can mimic the microenvironment of living vessels, to study the response of endothelial cells to stent placement. In this study, we constructed a digital pulse flow system based on a group of programmable solenoid valves, to mimic dynamic blood flows in the left coronary artery. Elastic artificial vessels, with internally cultured endothelial cells, were used to simulate vessel function and physiology. Based on this novel platform, we systematically explored cell proliferation and function in artificial vessels implanted with bare metal stents or drug-eluting stents, using unstented vessels as controls, under static and pulse flow conditions. The results indicate that the natural shear stresses of dynamic blood flow actually benefit endothelial cell attachment and proliferation. And drug-eluting stents showed stronger inhibition of cell proliferation than bare metal stents, but had a more negative effect on the synthesis of nitric oxide synthase (NOS), suggesting that drug elution might reduce the postoperative risk of restenosis, while increasing the risk of vasospasm. The results suggest that stent evaluation should include both the risk of restenosis and the effect on endothelial cells. Our simulation establishes a realistic in vitro model for pathological studies of restenosis and vasospasm, shows potential for evaluation of new stent designs, and could help develop individualised therapies for patients with atherosclerosis.
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http://dx.doi.org/10.1039/d0lc00533aDOI Listing
August 2020

DeepHelicon: Accurate prediction of inter-helical residue contacts in transmembrane proteins by residual neural networks.

J Struct Biol 2020 10 11;212(1):107574. Epub 2020 Jul 11.

Department of Bioinformatics, Wissenschaftzentrum Weihenstephan, Technische Universität München, 85354 Freising, Germany. Electronic address:

Accurate prediction of amino acid residue contacts is an important prerequisite for generating high-quality 3D models of transmembrane (TM) proteins. While a large number of compositional, evolutionary, and structural properties of proteins can be used to train contact prediction methods, recent research suggests that coevolution between residues provides the strongest indication of their spatial proximity. We have developed a deep learning approach, DeepHelicon, to predict inter-helical residue contacts in TM proteins by considering only coevolutionary features. DeepHelicon comprises a two-stage supervised learning process by residual neural networks for a gradual refinement of contact maps, followed by variance reduction by an ensemble of models. We present a benchmark study of 12 contact predictors and conclude that DeepHelicon together with the two other state-of-the-art methods DeepMetaPSICOV and Membrain2 outperforms the 10 remaining algorithms on all datasets and at all settings. On a set of 44 TM proteins with an average length of 388 residues DeepHelicon achieves the best performance among all benchmarked methods in predicting the top L/5 and L/2 inter-helical contacts, with the mean precision of 87.42% and 77.84%, respectively. On a set of 57 relatively small TM proteins with an average length of 298 residues DeepHelicon ranks second best after DeepMetaPSICOV. DeepHelicon produces the most accurate predictions for large proteins with more than 10 transmembrane helices. Coevolutionary features alone allow to predict inter-helical residue contacts with an accuracy sufficient for generating acceptable 3D models for up to 30% of proteins using a fully automated modeling method such as CONFOLD2.
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http://dx.doi.org/10.1016/j.jsb.2020.107574DOI Listing
October 2020

Comparison of Venous Thromboembolism Risks Between COVID-19 Pneumonia and Community-Acquired Pneumonia Patients.

Arterioscler Thromb Vasc Biol 2020 09 6;40(9):2332-2337. Epub 2020 Jul 6.

Division of Vascular and Endovascular Surgery, Department of Surgery, University of Washington and Puget Sound VA Health Care System, Seattle (W.W.Z.).

Objective: The objectives were to investigate and compare the risks and incidences of venous thromboembolism (VTE) between the 2 groups of patients with coronavirus disease 2019 (COVID-19) pneumonia and community-acquired pneumonia (CAP). Approach and Results: Medical records of 616 pneumonia patients who were admitted to the Yichang Central People's Hospital in Hubei, China, from January 1 to March 23, 2020, were retrospectively reviewed. The patients with COVID-19 pneumonia were treated in the dedicated COVID-19 units, and the patients with CAP were admitted to regular hospital campus. Risks of VTE were assessed using the Padua prediction score. All the patients received pharmaceutical or mechanical VTE prophylaxis. VTE was diagnosed using Duplex ultrasound or computed tomography pulmonary angiogram. Differences between COVID-19 and CAP groups were compared statistically. All statistical tests were 2 sided, and <0.05 was considered as statistically significant. All data managements and analyses were performed by IBM SPSS, version 24, software (SPSS, Inc, Chicago, IL). Of the 616 patients, 256 had COVID-19 pneumonia and 360 patients had CAP. The overall rate of VTE was 2% in COVID-19 pneumonia group and 3.6% in CAP group, respectively (=0.229). In these two groups, 15.6% of the COVID-19 pneumonia patients and 10% of the CAP patients were categorized as high risk for VTE (Padua score, >4), which were significantly different (=0.036). In those high-risk patients, the incidence of VTE was 12.5% in COVID-19 pneumonia group and 16.7% in CAP group (=0.606). Subgroup analysis of the critically ill patients showed that VTE rate was 6.7% in COVID-19 group versus 13% in CAP group (=0.484). In-hospital mortality of COVID-19 and CAP was 6.3% and 3.9%, respectively (=0.180).

Conclusions: Our study suggested that COVID-19 pneumonia was associated with hypercoagulable state. However, the rate of VTE in COVID-19 pneumonia patients was not significantly higher than that in CAP patients.
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http://dx.doi.org/10.1161/ATVBAHA.120.314779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7446987PMC
September 2020
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