Publications by authors named "Jianfei Wu"

24 Publications

  • Page 1 of 1

Genetic association analysis between IL9 and coronary artery disease in a Chinese Han population.

Cytokine 2021 Nov 20;150:155761. Epub 2021 Nov 20.

Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address:

Interleukin-9 (IL-9) plays important role in coronary artery disease (CAD). However, the exact relationship between them is not explored yet. Here, four tag SNPs covering IL9 (rs31563, rs2069868, rs2069870 and rs31564) were selected to conduct case-control association analyses in a total of 3704 individuals from Chinese Han population (1863 CAD vs 1841 control). Results showed that: first, rs2069868 was associated with CAD combined with hypertension (P = 0.027); second, IL9 haplotype (CGAT) was associated with CAD (P = 0.035), and the combination genotype of "rs31563_CC/rs31564_TT" would remarkably decrease the risk of CAD (P = 0.001); third, significant associations were found between rs2069870 and decreased LDL-c levels and decreased total cholesterol levels, and between rs31563 and increased HDL-c levels (P < 0.05). Therefore, we conclude that IL9 might play a causal role in CAD by interacted with CAD traditional risk factors, which might confer a new way to improve the prevention and treatment of CAD.
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http://dx.doi.org/10.1016/j.cyto.2021.155761DOI Listing
November 2021

Influence of targeted motivational interviewing on self-care level and prognosis during nursing care of chronic heart failure.

Am J Transl Res 2021 15;13(6):6576-6583. Epub 2021 Jun 15.

Department of Digestive Diseases, The First People's Hospital of Fuyang Hangzhou Hangzhou 311400, Zhejiang Province, China.

Objective: To analyze the effects of targeted motivational interviewing (TMI) during the nursing care for chronic heart failure (CHF).

Methods: A total of 93 patients with CHF admitted to our hospital were selected as study subjects, and were divided into Group A (n=47) and Group B (n=46) in accordance with a randomized double-blind method. Group A was treated with TMI, while Group B was treated with the conventional nursing care for heart failure (HF). The self-care levels (self-care of heart failure index), medication compliance (Morisky's medication compliance scale), quality of life (Minnesota Living with Heart Failure Questionnaire), and prognosis (the readmission and mortality rates) were compared between the 2 groups.

Results: After nursing care, patients in Group A were higher than those in Group B in their scores of self-care maintenance, management and confidence, medication frequency, time, dose, and type as instructed by the physician, and drug withdrawal or discontinuation of drugs, and forgetting medication, and body, emotion, and other fields ( < 0.05). Group A was superior to Group B in the rates of medication compliance and medication non-compliance (61.70% and 6.38% vs. 41.30% and 23.91%, < 0.05); rate of hospital readmission (6.38% vs. 23.91%, < 0.05), and mortality rate (0% vs. 6.52%, > 0.05).

Conclusion: TMI is conducive to improving the self-care level, medication compliance, quality of life, and prognosis in patients with CHF.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290814PMC
June 2021

The Trickle-Down Effect of Leaders' VWGB on Employees' Pro-Environmental Behaviors: A Moderated Mediation Model.

Front Psychol 2021 21;12:623687. Epub 2021 Apr 21.

International Institute of Finance, School of Management, University of Science and Technology of China, Hefei, China.

Although previous research has highlighted the positive effect of leaders' voluntary workplace green behavior (VWGB), limited research attention has been given to empirically testing how and when such behavior produces trickle-down effects. Taking a role model perspective and drawing on social identity theory, this research aims to fill this gap by proposing and testing the mechanism and boundary conditions of the influencing processes whereby leaders' VWGB can trickle down to employees' pro-environmental behaviors. By theorizing a moderated mediation model, the current research empirically examines the hypotheses by conducting a hierarchical regression analysis. We employed a survey questionnaire research design to collect two waves of multisource data. The data used in the analysis are from survey responses of 313 subordinate-supervisor dyads at two different time points. The results show that leaders' VWGB can have a trickle-down influence on employees' task-related pro-environmental behavior and proactive pro-environmental behaviors through their green self-identity and that this trickle-down effect is greater among employees with higher green climate perceptions. Our results reveal the intervening mechanism and boundary condition of leaders' VWGB by conducting a systematic examination of how this effect trickles down.
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http://dx.doi.org/10.3389/fpsyg.2021.623687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097181PMC
April 2021

High-Efficiency Electrolyte for Li-Rich Cathode Materials Achieving Enhanced Cycle Stability and Suppressed Voltage Fading Capable of Practical Applications on a Li-Ion Battery.

ACS Appl Mater Interfaces 2020 Nov 20;12(44):49666-49679. Epub 2020 Oct 20.

Qingdao Industrial Energy Storage Research Institute, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China.

Li-rich cathodes have been in considerable attention for their high reversible capacity. However, they have serious problems like poor cycling with intense capacity decay and voltage fading, which restrict their access to practical applications. In this work, a facile and efficient strategy is proposed to alleviate these intrinsic issues with a high-efficiency electrolyte system. This special electrolyte enables Li-rich cathodes to deliver superior integrated performance with a high initial discharge capacity of 301 mAh·g, outstanding cycling stability with a capacity retention of 88% at 0.5 C over 500 cycles, and a remarkable rate capability of 136 mAh·g at 5 C, respectively. What is more, the voltage fading is largely suppressed. Physical and electrochemical characterizations demonstrate that the robust CEI film formed on the cathode surface contributes to the improved electrochemical performance. This work provides a new approach to surmount defects of Li-rich materials and will largely promote their practical applications on Li-ion batteries.
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http://dx.doi.org/10.1021/acsami.0c14995DOI Listing
November 2020

Quantitative metabolome and transcriptome analysis reveals complex regulatory pathway underlying photoinduced fiber color formation in cotton.

Gene 2021 Jan 28;767:145180. Epub 2020 Sep 28.

State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, China. Electronic address:

As an important plant single cell model and textile application materials, poorly known about fiber color formation in cotton, which is sensitively regulated by environmental signals. Our studies underline the importance of photo signal on sensitive fiber color formation and characterize fiber color early initiation (15 DPA) and late accumulated metabolites (45 DPA) in different lighting condition. The results revealed 236 differential metabolites between control and shading, of which phenylpropanoids metabolites accounted for 20%, including uncharacterized novel metabolites and pathways. Furthermore, the early initiation specific genes respond to the absence of light are highly correlated with phenylpropanoid metabolites related to pigmentation. The current study reveals the complex pathways involving early initiation regulation and late metabolic pathways. In addition, the collection composed of uncharacterized photoinduced metabolites and early initiation signaling/regulatory genes were identified, which are important resources for understanding fiber color formation. This report provides new insight into molecular regulatory and biochemical basis underlying photoinduced fiber color formation in cotton.
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http://dx.doi.org/10.1016/j.gene.2020.145180DOI Listing
January 2021

Ectopic Expressions of the Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton.

Genes (Basel) 2020 07 9;11(7). Epub 2020 Jul 9.

State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, China.

The homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological function mode in plants. Based on the results of the current investigation, the gene crucially affects filament elongation and anther dehiscence of the stamen in cotton. Both excessive and lower expression of the gene lead to defective stamen development, resulting in shortened filaments and indehiscent anthers with pollen abortion. The results also showed that the phenotype of the shortened filaments was negatively correlated with anther defects in the seesaw model under the ectopic expression of . Moreover, our results notably indicated that the gene requires accurate expression and exhibits a sensitive dose effect for its proper function. This report has important fundamental and practical significance in crop science, and has crucial prospects for genetic engineering of new cytoplasmic male sterility lines and breeding of crop hybrid varieties.
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http://dx.doi.org/10.3390/genes11070772DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397050PMC
July 2020

Haploid Bio-Induction in Plant through Mock Sexual Reproduction.

iScience 2020 Jul 16;23(7):101279. Epub 2020 Jun 16.

State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, P. R. China. Electronic address:

Haploidization is invaluable for basic genetic research and crop breeding. The haploid bio-induction principle is an important topic that remains largely unexplored. In this study, both CenH3 RNAi and in vitro inhibition were used to simulate and induce haploids in allopolyploid crop. Notably, in vitro CenH3 inhibition showed that the results were much the same to that of RNAi in phenotype, chromosome behavior, microspore production, and haploid induction. Cytological analyses of RNAi and inhibitor-treated progenies revealed elimination of chromosomes, defective microspores with empty nuclei, thereby giving rise to pseudo male gametes, and haploid parthenogenesis induction. We found distinct defective empty microspores that were positively correlated with the decrease of CenH3 during RNAi manipulation. Investigation through both in vivo and in vitro studies revealed that haploidization was induced through the pseudo male gamete-mediated mock sexual reproduction. The present results provide insights for the haploid parthenogenesis induction process.
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http://dx.doi.org/10.1016/j.isci.2020.101279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334361PMC
July 2020

Dynamic Transcriptome Analysis Reveals Uncharacterized Complex Regulatory Pathway Underlying Genotype-Recalcitrant Somatic Embryogenesis Transdifferentiation in Cotton.

Genes (Basel) 2020 05 7;11(5). Epub 2020 May 7.

State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, China.

As a notable illustration of totipotency and plant regeneration, somatic embryogenesis (SE) is the developmental reprogramming of somatic cells toward the embryogenesis pathway, the key step for genetic engineering. Investigations examining the totipotency process are of great fundamental and practical importance in crop biotechnology. However, high-frequency regeneration of cotton via SE has been limited due to genotype-dependent response. The molecular basis deciphering SE genotype recalcitrance remains largely unexplored in cotton. In the current study, to comprehensively investigate the dynamic transcriptional profiling and gene regulatory patterns involved in SE process, a genome-wide RNA sequencing analysis was performed in two cotton genotypes with distinct embryogenic abilities, the highly embryogenic genotype Yuzao 1 (YZ) and the recalcitrant genotype Lumian 1 (LM). Three typical developmental staged cultures of early SE-hypocotyls (HY), nonembryogenic calli (NEC) and primary embryogenic calli (PEC)-were selected to establish the transcriptional profiles. Our data revealed that a total of 62,562 transcripts were present amongst different developmental stages in the two genotypes. Of these, 18,394 and 26,514 differentially expressed genes (DEGs) were identified during callus dedifferentiation (NEC-VS-HY) and embryogenic transdifferentiation (PEC-VS-NEC), respectively in the recalcitrant genotype, 21,842 and 22,343 DEGs in the highly embryogenic genotype. Furthermore, DEGs were clustered into six expression patterns during cotton SE process in the two genotypes. Moreover, functional enrichment analysis revealed that DEGs were significantly enriched in fatty acid, tryptophan and pyruvate metabolism in the highly embryogenic genotype and in DNA conformation change otherwise in the recalcitrant genotype. In addition, critical SE-associated expressed transcription factors, as well as alternative splicing events, were notably and preferentially activated during embryogenic transdifferentiation in the highly embryogenic genotype compared with the recalcitrant genotype. Taken together, by systematically comparing two genotypes with distinct embryogenic abilities, the findings in our study revealed a comprehensive overview of the dynamic gene regulatory patterns and uncharacterized complex regulatory pathways during cotton SE genotype-dependent response. Our work provides insights into the molecular basis and important gene resources for understanding the underlying genotype recalcitrance during SE process and plant regeneration, thereby holding great promise for accelerating the application of biotechnology to cotton for improving its breeding efficiency.
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http://dx.doi.org/10.3390/genes11050519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290922PMC
May 2020

Perylene Diimide-Based Conjugated Polymers for All-Polymer Solar Cells.

Chemistry 2020 Oct 11;26(55):12510-12522. Epub 2020 Aug 11.

College of Materials Science and Opto-Electronic Technology &, Center of Materials Science and Optoelectronics Engineering &, CAS Center for Excellence in Topological Quantum Computation &, CAS Key Laboratory of Vacuum Physics, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.

In recent decades, non-fullerene acceptors (NFAs) are undergoing rapid development and emerging as a hot area in the field of organic solar cells. Among the high-performance non-fullerene acceptors, aromatic diimide-based electron acceptors remain to be highly promising systems. This review discusses the important progress of perylene diimide (PDI)-based polymers as non-fullerene acceptors in all-polymer solar cells (all-PSCs) since 2014. The relationship between structure and property, matching aspects between donors and acceptors, and device fabrications are unveiled from a synthetic chemist perspective.
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http://dx.doi.org/10.1002/chem.202001011DOI Listing
October 2020

Ethyl methanesulfonate mutant library construction in Gossypium hirsutum L. for allotetraploid functional genomics and germplasm innovation.

Plant J 2020 07 28;103(2):858-868. Epub 2020 Apr 28.

State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, China.

As the gene pool is exposed to both strain on land resources and a lack of diversity in elite allotetraploid cotton, the acquisition and identification of novel alleles has taken on epic importance in facilitating cotton genetic improvement and functional genomics research. Ethyl methanesulfonate (EMS) is an excellent mutagen that induces genome-wide efficient mutations to activate the mutagenic potential of plants with many advantages. The present study established, determined and verified the experimental procedure suitable for EMS-based mutant library construction as the general reference guide in allotetraploid upland cotton. This optimized method and procedure are efficient, and abundant EMS mutant libraries (approximately 12 000) in allotetraploid cotton were successfully obtained. More than 20 mutant phenotypes were observed and screened, including phenotypes of the leaf, flower, fruit, fiber and plant architecture. Through the plants mutant library, high-throughput and high-resolution melting technology-based variation evaluation detected the EMS-induced site mutation. Additionally, based on overall genome-wide mutation analyses by re-sequencing and mutant library assessment, the examination results demonstrated the ideal quality of the cotton EMS-treated mutant library constructed in this study with appropriate high mutation density and saturated genome. What is more, the collection is composed of a broad repertoire of mutants, which is the valuable resource for basic genetic research and functional genomics underlying complex allotetraploid traits, as well as cotton breeding.
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http://dx.doi.org/10.1111/tpj.14755DOI Listing
July 2020

Comparison of Percutaneous Transforaminal Endoscopic Discectomy with and without Epidural Steroid Application in the Treatment of Lumbar Disc Herniation: A Minimum of 2 Years of Follow-Up.

Turk Neurosurg 2020 ;30(3):387-393

The Affiliated Hospital of Putian University, Department of Orthopaedics, Putian, Fujian, China.

Aim: To compare the efficacy of percutaneous transforaminal endoscopic discectomy with and without epidural steroid application in the treatment of lumbar disc herniation.

Material And Methods: A total of 101 patients who had indications for percutaneous transforaminal endoscopic discectomy were retrospectively reviewed. Patients were divided into two groups based on whether epidural steroids were applied following the surgical procedure. Each patient in group A received an epidural injection of 40mg triamcinolone acetonide through the working sleeve following the surgical procedure. However, patients in group B were not given the drug. Preoperative and postoperative radicular pain was measured with the visual analogue scale (VAS). Functional and satisfaction outcomes were measured with the Oswestry Disability Index (ODI) and the modified MacNab criteria, respectively.

Results: A total of 97 patients had a complete follow-up of longer than two years without recurrence. The sample sizes of group A and group B were 56 and 41. The VAS scores and ODI scores at each postoperative follow-up point were significantly lower than the preoperative values (p < 0.001). However, there were no significant between-group differences (p > 0.05). The secondary outcomes of the changes in the VAS and ODI scores were also equivalent between the groups at the 3-month and 2-year follow-ups (p > 0.05). No significant difference was observed in the modified MacNab criteria between the groups at 2 years (p=0.7715).

Conclusion: Percutaneous transforaminal endoscopic discectomy is a safe and effective minimally invasive surgery for radiculopathy caused by lumbar disc herniation. Epidural steroid injection following the surgical procedure offered no benefit compared with surgery alone.
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http://dx.doi.org/10.5137/1019-5149.JTN.26972-19.2DOI Listing
October 2020

miR-497 inhibits the carcinogenesis of hepatocellular carcinoma by targeting the Rictor/Akt signal pathway.

Int J Clin Exp Pathol 2019 1;12(6):1992-2000. Epub 2019 Jun 1.

Department of Internal Medicine-Oncology, The Affilicated Hosptial of Hebei University Hebei, China.

MicroRNAs (miRNAs) are involved in regulating various physiologic and pathologic processes of different human diseases including hepatocellular carcinoma (HCC). Our research aimed to investigate the role of miR-497 in migration, invasive ability of HepG2-GS cells and the regulating mechanism. In this study, Rictor was identified as a target gene of miR-497 by informatic software, including Microcosm Targets, miRanda, and TargetScan. MiR-497 or Rictor were silenced or overexpressed in HepG2-GS cells through transfection. The functional assay results showed that Rictor knockdown inhibited cancer cell proliferation, migration and invasion. Overexpression of Rictor inversed the effects of miR-497 on cancer cells growth inhibition. miR-497 regulated protein kinase B, PKB (Akt) signaling pathway by targeting Rictor. MiR-497 increased chemo-sensitivity of HepG2-GS through regulation of Rictor. In conclusion, our research demonstrated that miR-497 inhibits the proliferation, invasion, metastasis, and chemotherapy resistance of hepatoma cells by targeting of Rictor/Akt signal pathway, and miR-497. Thus, Rictor has the potential to be a explored as a biomarker or therapeutic target for diagnosis and treatment of HCC.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949653PMC
June 2019

The intensification of metallic layered phenomena above thunderstorms through the modulation of atmospheric tides.

Sci Rep 2019 Nov 29;9(1):17907. Epub 2019 Nov 29.

Key Laboratory of Middle Atmosphere and Global Environment Observation, Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing, China.

We present a multi-instrument experiment to study the effects of tropospheric thunderstorms on the mesopause region and the lower ionosphere. Sodium (Na) lidar and ionospheric observations by two digital ionospheric sounders are used to study the variation in the neutral metal atoms and metallic ions above thunderstorms. An enhanced ionospheric sporadic E layer with a downward tidal phase is observed followed by a subsequent intensification of neutral Na number density with an increase of 600 cm in the mesosphere. In addition, the Na neutral chemistry and ion-molecule chemistry are considered in a Na chemistry model to simulate the dynamical and chemical coupling processes in the mesosphere and ionosphere above thunderstorms. The enhanced Na layer in the simulation obtained by using the ionospheric observation as input is in agreement with the Na lidar observation. We find that the intensification of metallic layered phenomena above thunderstorms is associated with the atmospheric tides, as a result of the troposphere-mesosphere-ionosphere coupling.
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http://dx.doi.org/10.1038/s41598-019-54450-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884582PMC
November 2019

Microwave-Assisted Classic Ullmann C-C Coupling Polymerization for Acceptor-Acceptor Homopolymers.

Polymers (Basel) 2019 Oct 24;11(11). Epub 2019 Oct 24.

College of Materials Science and Opto-Electronic Technology & Center of Materials Science and Optoelectronics Engineering & CAS Center for Excellence in Topological Quantum Computation & CAS Key Laboratory of Vacuum Physic, University of Chinese Academy of Sciences, Beijing 100049, China.

Developing cheap, clean and atomic-efficient synthetic methodologies for conjugated polymers are always critical for the field of organic electronics. Herein, classic Ullmann coupling polymerization is developed to synthesize a series of Acceptor-Acceptor (A-A) type homopolymers with microwave-assistance, which are supported by nuclear magnetic resonance (NMR), matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF), elemental analysis (EA) and gel permeation chromatography (GPC). The physicochemical properties of these polymers are studied by UV-vis spectroscopy, cyclic voltammetry (CV), thermal gravimetric analysis (TGA), and density functional theory (DFT) calculation. Furthermore, these A-A homopolymers are used as acceptors for all-polymer solar cells (All-PSCs), affording a promising efficiency of 3.08%, which is the highest value for A-A-homopolymer-based organic solar cells.
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http://dx.doi.org/10.3390/polym11111741DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918342PMC
October 2019

Meiosis, the master driver of gene duplication in higher plants?

Biochem Biophys Res Commun 2019 06 9;514(3):756-758. Epub 2019 May 9.

State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, 271018, PR China. Electronic address:

Meiosis is a critical biological process for reproduction and genetic variation in higher plants. Gene duplication is a prominent feature of plant genomic architecture. Meiosis and gene duplication are of fundamental importance in unraveling the nature of genetics and evolution. The ideas and findings in this letter demonstrate a highly significant connection between meiosis and gene duplication, bring together these two disparate fields of study and highlight the importance of meiosis for understanding the evolutionary success of flowering plants. These insights and opinions open a new area of investigation and point to a significant way to illustrate the impact of duplicated genes on meiosis and fitness in higher plants, as well as their ultimate evolutionary, ecological, and agronomic impacts in light of challenges that have arisen due to global climate change. This study addresses novel ideas and viewpoints in plant developmental genomics and evolution.
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http://dx.doi.org/10.1016/j.bbrc.2019.04.127DOI Listing
June 2019

Metabolome and Transcriptome Association Analysis Reveals Dynamic Regulation of Purine Metabolism and Flavonoid Synthesis in Transdifferentiation during Somatic Embryogenesis in Cotton.

Int J Mol Sci 2019 Apr 26;20(9). Epub 2019 Apr 26.

State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, China.

Plant regeneration via somatic embryogenesis (SE) is a key step during genetic engineering. In the current study, integrated widely targeted metabolomics and RNA sequencing were performed to investigate the dynamic metabolic and transcriptional profiling of cotton SE. Our data revealed that a total of 581 metabolites were present in nonembryogenic staged calli (NEC), primary embryogenic calli (PEC), and initiation staged globular embryos (GE). Of the differentially accumulated metabolites (DAMs), nucleotides, and lipids were specifically accumulated during embryogenic differentiation, whereas flavones and hydroxycinnamoyl derivatives were accumulated during somatic embryo development. Additionally, metabolites related to purine metabolism were significantly enriched in PEC vs. NEC, whereas in GE vs. PEC, DAMs were remarkably associated with flavonoid biosynthesis. An association analysis of the metabolome and transcriptome data indicated that purine metabolism and flavonoid biosynthesis were co-mapped based on the Kyoto encyclopedia of genes and genomes (KEGG) database. Moreover, purine metabolism-related genes associated with signal recognition, transcription, stress, and lipid binding were significantly upregulated. Moreover, several classic somatic embryogenesis (SE) genes were highly correlated with their corresponding metabolites that were involved in purine metabolism and flavonoid biosynthesis. The current study identified a series of potential metabolites and corresponding genes responsible for SE transdifferentiation, which provides a valuable foundation for a deeper understanding of the regulatory mechanisms underlying cell totipotency at the molecular and biochemical levels.
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http://dx.doi.org/10.3390/ijms20092070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539419PMC
April 2019

Emodin, a natural anthraquinone, suppresses liver cancer in vitro and in vivo by regulating VEGFR and miR-34a.

Invest New Drugs 2020 04 11;38(2):229-245. Epub 2019 Apr 11.

Department of Hepatobiliary Surgery, the Fourth Hospital of Hebei Medical University, NO.12, Jiankang Road, Shijiazhuang, 050011, Hebei Province, People's Republic of China.

The pharmacokinetic (PK) and potential effects of Emodin on liver cancer were systematically evaluated in this study. Both the intragastric administration (i.g.) and hypodermic injection (i.h.) of Emodin exhibited a strong absorption (absorption rate < 1 h) and elimination capacity (t ≈ 2 h). The tissue distribution of Emodin after i.h. was rapid and wide. The stability of Emodin in three species of liver microsomes wasrat >human> beagle dog. These PK data provided the basis for the subsequent animal experiments. In liver cancer patient tissues, the expression of vascular endothelial growth factor (VEGF)-induced signaling pathways, including phosphorylated VEGF receptor 2 (VEGFR), AKT, and ERK,were simultaneously elevated, but miR-34a expression was reduced and negatively correlated with SMAD and SMAD. Emodin inhibited the expression of SMAD in HepG2 cells by inducing the miR-34a level. Subsequently, BALB/c nude mice received a daily subcutaneous injection of HepG2 cells with or without Emodin treatment (1 mg/kg or 10 mg/kg), and Emodin inhibited tumorigenesis and reduced the mortality rate in a dose-dependent manner. In vivo experiments showed that cell proliferation, migration, and invasion were promoted by VEGF or miR-34a signal treatment but were inhibited when combined with Emodin treatment. All these results demonstrated that Emodin inhibited tumorigenesis in liver cancer by simultaneously inhibiting the VEGFR-AKT-ERKsignaling pathway and promoting a miR-34a-mediated signaling pathway.
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http://dx.doi.org/10.1007/s10637-019-00777-5DOI Listing
April 2020

Reliable Interlayer Based on Hybrid Nanocomposites and Carbon Nanotubes for Lithium-Sulfur Batteries.

ACS Appl Mater Interfaces 2019 May 22;11(17):15607-15615. Epub 2019 Apr 22.

Qingdao Institute of Bioenergy and Bioprocess Technology , Chinese Academy of Sciences , Qingdao 266101 , PR China.

The future energy needs have triggered research interest in finding novel energy storage systems with high energy density. Lithium-sulfur batteries are regarded as one of the most promising options for the next-generation energy storage applications because of their high theoretical energy and low cost. However, the electrochemical performances of lithium-sulfur batteries are seriously compromised by the polysulfide (LiPS) shuttling and the insulating nature of sulfur. To overcome these issues, novel CoNiFeO (CNFO) nanoparticles uniformly covered on the carbon nanotubes are now reported as an efficient functional interlayer. Benefiting from the sufficient sulfiphilic sites of the CNFO for chemically bonding with LiPSs, as well as the conductive interconnected skeleton of carbon nanotubes, this composite material showed great enhancement on the rate capability and cycle stability of Li-S batteries. The Li-S battery using this interlayer exhibited a high initial capacity of 897 mA h g and a low capacity decay of 0.063% per cycle within 250 cycles at 2 C. Meanwhile, an reversible specific capacity of 869 mA h g (at 0.5 C) with high Coulombic efficiency could be obtained over 100 cycles at an elevated temperature (60 °C). We speculated that the chemical adsorption of CNFO for polysulfide-anchoring is extremely critical for the performances of Li-S batteries under high temperature.
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http://dx.doi.org/10.1021/acsami.9b02136DOI Listing
May 2019

Iris-Like Acceptor with Most PDI Units for Organic Solar Cells.

ACS Appl Mater Interfaces 2018 Aug 17;10(34):28812-28818. Epub 2018 Aug 17.

College of Materials Science and Opto-Electronic Technology & Key Laboratory of Vacuum Physic , University of Chinese Academy of Sciences , Beijing 100049 , P. R. China.

Mother Nature is always the best source for scientists to draw inspiration. Herein, a three-dimensional perylene diimide (PDI)-based molecular acceptor was designed and synthesized, in which six PDI units form an "iris-like" structure upon connecting with the hexaphenylbenzene core. Interestingly, this molecule is the nonfullerene acceptor containing most PDI units, which can absorb solar light to exhibit excellent power conversion efficiency, much more efficient than the natural flowers. This contribution presents an interesting example of learning from Mother Nature to design novel materials for applications.
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http://dx.doi.org/10.1021/acsami.8b10347DOI Listing
August 2018

Type I Diabetic Akita Mouse Model is Characterized by Abnormal Cardiac Deformation During Early Stages of Diabetic Cardiomyopathy with Speckle-Tracking Based Strain Imaging.

Cell Physiol Biochem 2018 21;45(4):1541-1550. Epub 2018 Feb 21.

Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China.

Background/aims: Diabetes mellitus (DM) has been demonstrated to have a strong association with heart failure. Conventional echocardiographic analysis cannot sensitively monitor cardiac dysfunction in type I diabetic Akita hearts, but the phenotype of heart failure is observed in molecular levels during the early stages.

Methods: Male Akita (Ins2WT/C96Y) mice were monitored with echocardiographic imaging at various ages, and then with conventional echocardiographic analysis and speckle-tracking based strain analyses.

Results: With speckle-tracking based strain analyses, diabetic Akita mice showed changes in average global radial strain at the age of 12 weeks, as well as decreased longitudinal strain. These changes occurred in the early stage and remained throughout the progression of diabetic cardiomyopathy in Akita mice. Speckle-tracking showed that the detailed and precise changes of cardiac deformation in the progression of diabetic cardiomyopathy in the genetic type I diabetic Akita mice were uncoupled.

Conclusions: We monitored early-stage changes in the heart of diabetic Akita mice. We utilize this technique to elucidate the underlying mechanism for heart failure in Akita genetic type I diabetic mice. It will further advance the assessment of cardiac abnormalities, as well as the discovery of new drug treatments using Akita genetic type I diabetic mice.
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http://dx.doi.org/10.1159/000487690DOI Listing
May 2018

Triplet Tellurophene-Based Acceptors for Organic Solar Cells.

Angew Chem Int Ed Engl 2018 01 4;57(4):1096-1102. Epub 2018 Jan 4.

College of Materials Science and Optoelectronic Technology & CAS Key Laboratory of Vacuum Physics, University of Chinese Academy of Sciences, Beijing, 101408, P. R. China.

Triplet materials have been employed to achieve high-performing organic solar cells (OSCs) by extending the exciton lifetime and diffusion distances, while the triplet non-fullerene acceptor materials have never been reported for bulk heterojunction OSCs. Herein, for the first time, three triplet molecular acceptors based on tellurophene with different degrees of ring fusing were designed and synthesized for OSCs. Significantly, these molecules have long exciton lifetime and diffusion lengths, leading to efficient power conversion efficiency (7.52 %), which is the highest value for tellurophene-based OSCs. The influence of the extent of ring fusing on molecular geometry and OSCs performance was investigated to show the power conversion efficiencies (PCEs) continuously increased along with increasing the extent of ring fusing.
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http://dx.doi.org/10.1002/anie.201712011DOI Listing
January 2018

Environmental and genetic factors that contribute to Escherichia coli K-12 biofilm formation.

Arch Microbiol 2010 Sep 18;192(9):715-28. Epub 2010 Jun 18.

Department of Veterinary and Microbiological Sciences, North Dakota State University, Fargo, ND 58108-6050, USA.

Biofilms are communities of bacteria whose formation on surfaces requires a large portion of the bacteria's transcriptional network. To identify environmental conditions and transcriptional regulators that contribute to sensing these conditions, we used a high-throughput approach to monitor biofilm biomass produced by an isogenic set of Escherichia coli K-12 strains grown under combinations of environmental conditions. Of the environmental combinations, growth in tryptic soy broth at 37 degrees C supported the most biofilm production. To analyze the complex relationships between the diverse cell-surface organelles, transcriptional regulators, and metabolic enzymes represented by the tested mutant set, we used a novel vector-item pattern-mining algorithm. The algorithm related biofilm amounts to the functional annotations of each mutated protein. The pattern with the best statistical significance was the gene ontology 'pyruvate catabolic process,' which is associated with enzymes of acetate metabolism. Phenotype microarray experiments illustrated that carbon sources that are metabolized to acetyl-coenzyme A, acetyl phosphate, and acetate are particularly supportive of biofilm formation. Scanning electron microscopy revealed structural differences between mutants that lack acetate metabolism enzymes and their parent and confirmed the quantitative differences. We conclude that acetate metabolism functions as a metabolic sensor, transmitting changes in environmental conditions to biofilm biomass and structure.
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http://dx.doi.org/10.1007/s00203-010-0599-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923660PMC
September 2010

Relating gene expression data on two-component systems to functional annotations in Escherichia coli.

BMC Bioinformatics 2008 Jun 25;9:294. Epub 2008 Jun 25.

Department of Computer Science and Operations Research, North Dakota State University, Fargo, ND 58105, USA.

Background: Obtaining physiological insights from microarray experiments requires computational techniques that relate gene expression data to functional information. Traditionally, this has been done in two consecutive steps. The first step identifies important genes through clustering or statistical techniques, while the second step assigns biological functions to the identified groups. Recently, techniques have been developed that identify such relationships in a single step.

Results: We have developed an algorithm that relates patterns of gene expression in a set of microarray experiments to functional groups in one step. Our only assumption is that patterns co-occur frequently. The effectiveness of the algorithm is demonstrated as part of a study of regulation by two-component systems in Escherichia coli. The significance of the relationships between expression data and functional annotations is evaluated based on density histograms that are constructed using product similarity among expression vectors. We present a biological analysis of three of the resulting functional groups of proteins, develop hypotheses for further biological studies, and test one of these hypotheses experimentally. A comparison with other algorithms and a different data set is presented.

Conclusion: Our new algorithm is able to find interesting and biologically meaningful relationships, not found by other algorithms, in previously analyzed data sets. Scaling of the algorithm to large data sets can be achieved based on a theoretical model.
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http://dx.doi.org/10.1186/1471-2105-9-294DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2478693PMC
June 2008
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