Publications by authors named "Jian-Ying Guo"

30 Publications

  • Page 1 of 1

AQP2 as a target of lycopene protects against atrazine-induced renal ionic homeostasis disturbance.

Food Funct 2021 Jun;12(11):4855-4863

College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, P. R. China. and Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, P. R. China and Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Northeast Agricultural University, Harbin, 150030, P. R. China.

Atrazine (ATR), a ubiquitous environmental contaminant in water and soil, causes environmental nephrosis. To reveal the toxic effect of ATR on the kidney and the potential chemical nephroprotective effect of lycopene (LYC), Kun-Ming mice of specific pathogen-free (SPF) grade were treated with LYC (5 mg kg-1) and/or ATR (50 mg kg-1 or 200 mg kg-1) for 21 days. The degree of renal injury was evaluated by measuring the ion concentration, ATPase activities and the mRNA expressions/levels of associated ATPase subunits. In addition, the expression of renal aquaporins (AQPs) was analyzed. The results showed that the renal tubular epithelial cells of ATR-exposed mice were swollen, the glomeruli were significantly atrophied, and the ion concentrations were obviously changed. The activity of Na+-K+-ATPase and the transcription of its subunits were downregulated. The activity of Ca2+-Mg2+-ATPase and the transcription of its subunits were upregulated. The expression of AQPs, especially the critical AQP2, was affected. Notably, ATR-induced nephrotoxicity was significantly improved by LYC supplementation. Therefore, LYC could protect the kidney against ATR-induced nephrotoxicity via maintaining ionic homeostasis, reversing the changes in ATPase activity and controlling the expression of AQPs on the cell membrane. These results suggested that AQP2 was a target of LYC and protected against ATR-induced renal ionic homeostasis disturbance.
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http://dx.doi.org/10.1039/d0fo03214jDOI Listing
June 2021

Co-selection may explain the unexpectedly high prevalence of plasmid-mediated colistin resistance gene in a Chinese broiler farm.

Zool Res 2020 Sep;41(5):569-575

College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, China. E-mail:

The rise of the plasmid-encoded colistin resistance gene is a major concern globally. Here, during a routine surveillance, an unexpectedly high prevalence of with reduced susceptibility to colistin (69.9%) was observed in a Chinese broiler farm. Fifty-three (63.9%) isolates were positive for . All identified -positive (MCREC) were multidrug resistant and carried other clinically significant resistance genes. Furthermore, the genes were mainly located on the IncI2 and IncHI2 plasmids. Conjugation experiments unraveled the co-transfer of with other antibiotic resistance genes (, , , and ) via the IncI2 (=3) and IncHI2 (=4) plasmids. The stable genetic context - was common in the IncI2 plasmids, whereas IS---IS was mainly found in the IncHI2 plasmids. The dominance of -bearing IncI2 and IncHI2 plasmids and co-selection of with other antimicrobial resistance genes might contribute to the exceptionally high prevalence of in this broiler farm. Our results emphasized the importance of appropriate antibiotic use in animal production.
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http://dx.doi.org/10.24272/j.issn.2095-8137.2020.131DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475015PMC
September 2020

Multiple Plasmid Vectors Mediate the Spread of in Extended-Spectrum-β-Lactamase-Producing Isolates from Retail Vegetables in China.

mSphere 2020 07 15;5(4). Epub 2020 Jul 15.

College of Veterinary Medicine, National Risk Assessment Laboratory for Antimicrobial Resistant of Microorganisms in Animals, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China

The plasmid-mediated fosfomycin resistance gene has been detected in from various sources but has rarely been reported in vegetables. In this study, the aim was to investigate the prevalence of and, subsequently, to characterize -positive isolates from retail vegetables. Seventeen (7.3%) -carrying strains were identified from 233 extended-spectrum-β-lactamase-producing isolates from vegetables. All 17 isolates, including six , seven , two , and two isolates, carried S1-nuclease pulsed-field gel electrophoresis (S1-PFGE) and hybridization confirmed that the genes in 16 isolates were located on plasmids ranging in size from ∼40 kb to ∼250 kb, except one located on chromosome of All the carrying plasmids from 16 -positive isolates were successfully transferred into the recipient bacteria by transformation or conjugation. In agreement with data determined with isolates from food animals, the IncHI2/ST3 and IncN-F33:A-:B-/F33:A-:B plasmids were the main vectors of in Additionally, F24:A-:B1, IncFII-IncR, IncFII, IncR, and two untypeable plasmids were found for the first time to be vectors for in The genetic contexts of in 15 isolates differed due to insertion and/or loss of molecular modules mediated by mobile elements. However, all genes were flanked by IS, as commonly observed in other -carrying plasmids. Here, we report a high rate of detection of genes, mediated by multiple plasmid vectors, in ESBL-producing from retail vegetables. FosA3-producing could be transmitted to the human body by direct contact or consumption of vegetables, which might pose a potential threat to public health. This report provides important information on the transmission and epidemiology of among isolates from vegetables. The rate of occurrence of in ESBL-producing from retail vegetables is high, and was found to be carried by diverse plasmids. Some novel genetic contexts of and novel -carrying plasmids, including several plasmid types common in , were identified, increasing the number of known transfer vectors for the gene and reflecting the complexity of transmission in The capture of by the resident plasmid of will accelerate the spread of in , one of the most pathogenic species in clinical medicine. Considering the clinical importance of fosfomycin, and the fact that vegetables are directly consumed, the fosfomycin resistance genes present a risk of transmission to the human body through the food chain and thus pose a threat to public health.
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http://dx.doi.org/10.1128/mSphere.00507-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7364219PMC
July 2020

Sox genes in Agasicles hygrophila (Coleoptera: Chrysomelidae) are involved in ovarian development and oogenesis.

Arch Insect Biochem Physiol 2020 Sep 17;105(1):e21721. Epub 2020 Jun 17.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

The alligator weed flea beetle, Agasicles hygrophila is a monophagous natural enemy of the invasive alligator weed Alternanthera philoxeroides. Oogenesis plays a vital role in the process of individual development and population continuation of oviparous insects. Sox is an ancient and ubiquitous metazoan gene family that plays a key regulatory role in various physiological processes, including oogenesis, which is closely related to fecundity. In this study, two Sox genes AhDichaete and AhSox3 were cloned and characterized, and then the expression profiles of AhDichaete and AhSox3 were qualified by a quantitative reverse transcription-polymerase chain reaction. The result showed that these two Sox genes were expressed significantly higher in ovary, especially in the adult developmental stage. Furthermore, the functions of AhDichaete and AhSox3 in A. hygrophila females were studied using RNA interference (RNAi). Fewer offsprings were produced when AhDichaete and AhSox3 RNAi females mated with wild-type males. Moreover, dsAhSox3 injection reduced the hatching rate of eggs but injection with dsAhDichaete did not. Further study of the reproductive system of AhDichaete and AhSox3 RNAi females showed that yolk protein deposition reduction in the ovarioles, then the expression of vitellogenin gene AhVg2 in ovaries was decreased. These results indicate that AhDichaete and AhSox3 play an important regulatory role in the process of ovarian development and oogenesis by affecting yolk synthesis in the ovary of A. hygrophila.
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http://dx.doi.org/10.1002/arch.21721DOI Listing
September 2020

Di (2-ethyl hexyl) phthalate (DEHP)-induced spleen toxicity in quail (Coturnix japonica) via disturbing Nrf2-mediated defense response.

Environ Pollut 2019 Aug 15;251:984-989. Epub 2019 May 15.

College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, PR China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, PR China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Northeast Agricultural University, Harbin, 150030, PR China. Electronic address:

Di(2-ethylhexyl) phthalate (DEHP), as a widely used plasticizer, is reported to have widespread environmental and global health hazards. Trace amounts of phthalates in the environment are sufficient to disrupt ecological balance and affect human health. However, DEHP-induced splenic toxicity remains in an unknown state. Therefore, to explore the mechanism of DEHP-induced splenic toxicity, male quail were employed with 0, 250, 500 and 750 mg/kg body weight DEHP by daily gastric perfusion for 45 days. Notably, splenic corpuscular border and cell gap enlargement were observed in the spleen tissue of DEHP-exposed quail under the histopathological analysis. Furthermore, DEHP induced dysregulation of oxidative stress markers by increasing malondialdehyde (MDA) content and decreasing superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities. Low concentration of DEHP (≤250 mg/kg) exposure suppressed nuclear factor-E2-related factor 2 (Nrf2) signaling pathway, while high concentration of DEHP (≥500 mg/kg) exposure activated Nrf2-mediated defense response. DEHP induced splenic oxidative stress via interfering Nrf2 signal pathway and altering the transcription of its downstream genes. In conclusion, this study suggested that DEHP induced splenic toxicity.
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http://dx.doi.org/10.1016/j.envpol.2019.05.061DOI Listing
August 2019

Lycopene Triggers Nrf2-AMPK Cross Talk to Alleviate Atrazine-Induced Nephrotoxicity in Mice.

J Agric Food Chem 2018 Nov 9;66(46):12385-12394. Epub 2018 Nov 9.

College of Veterinary Medicine , ‡Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment , and §Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine , Northeast Agricultural University , Harbin 150030 , P.R. China.

Atrazine (ATR), an environmental persistent and bioaccumulative herbicide, has been associated with environmental nephrosis. Lycopene (LYC) exhibits important properties of nephroprotection, but there are limited data on the specific underlying mechanism. The primary objective of this study was to explore the therapeutic effect of LYC on ATR-induced nephrotoxicity in mice. The mice were divided randomly into 6 groups and treated as follows: control group (C), 5 mg/kg LYC group (L), 50 mg/kg ATR group (A1), 200 mg/kg ATR group (A2), 50 mg/kg ATR plus 5 mg/kg LYC group (A1+L), and 200 mg/kg ATR plus 5 mg/kg LYC group (A2+L) by oral gavage administration for 21 days. We found that pretreatment with LYC significantly suppressed the ATR-induced renal tubular epithelial cell swelling. Furthermore, LYC mitigated ATR-induced dysregulation of oxidative stress markers by reducing MDA, HO levels, and increasing SOD, GPx, CAT concentration, and Nrf2 activation. Moreover, LYC activated the autophagic flux by a detectable change in autophagy-related genes (Beclin-1 and ATGs) and proteins (p62/SQSTM) and by the formation of autophagic vacuole (AV) and LC3 aggregation, in parallel with AMPK activation (pAMPK/AMPK). Herein, ATR-up-regulated nuclear factor erythroid 2-related factor 2 (Nrf2) expression and Nrf2-regulated redox genes, including quinoneoxidoreductase-1 (NQO1) and heme oxidase-1 (HO1), whereas LYC down-regulated those of the above genes. In addition, LYC suppressed ATR-induced activation of autophagy (increased LC3II/LC3I, ATGs, Beclin1, and p62, in parallel with increased AMPK activation). Collectively, our findings identified a cross talk between AMPK-activated autophagy and the Nrf2 signaling pathway in LYC-mediated nephroprotection against ATR-induced toxicity in mice kidney.
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http://dx.doi.org/10.1021/acs.jafc.8b04341DOI Listing
November 2018

Investigation of myocardial dysfunction using three-dimensional speckle tracking echocardiography in a genetic positive hypertrophic cardiomyopathy Chinese family.

Cardiol Young 2018 Sep 6;28(9):1106-1114. Epub 2018 Jul 6.

1Department of Ultrasound,Xijing Hospital,Fourth Military Medical University,Xi'an,Shaanxi,China.

Background: We previously reported four heterozygous missense mutations of MYH7, KCNQ1, MYLK2, and TMEM70 in a single three-generation Chinese family with dual Long QT and hypertrophic cardiomyopathy phenotypes for the first time. However, the clinical course among the family members was various, and the potential myocardial dysfunction has not been investigated.

Objectives: The objective of this study was to investigate the echocardiographic and electrocardiographic characteristics in a genetic positive Chinese family with hypertrophic cardiomyopathy and further to explore the association between myocardial dysfunction and electric activity, and the identified mutations.

Methods: A comprehensive echocardiogram - standard two-dimensional Doppler echocardiography and three-dimensional speckle tracking echocardiography - and electrocardiogram were obtained for members in this family.

Results: As previously reported, four missense mutations - MYH7-H1717Q, KCNQ1-R190W, MYLK2-K324E, and TMEM70-I147T - were identified in this family. The MYH7-H1717Q mutation carriers had significantly increased left ventricular mass indices, elevated E/e' ratio, deteriorated global longitudinal stain, but enhanced global circumferential and radial strain compared with those in non-mutation patients (all p<0.05). The KCNQ1-R190W carriers showed significantly prolonged QTc intervals, and the MYLK2-K324E mutation carriers showed inverted T-waves (both p<0.05). However, the TMEM70-I147T mutation carriers had similar echocardiography and electrocardiographic data as non-mutation patients.

Conclusions: Three of the identified four mutations had potential pathogenic effects in this family: MYH7-H1717Q was associated with increased left ventricular thickness, elevated left ventricular filling pressure, and altered myocardial deformation; KCNQ1-R190W and MYLK2-K324E mutations were correlated with electrocardiographic abnormalities reflected in long QT phenotype and inverted T-waves, respectively.
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http://dx.doi.org/10.1017/S1047951118000860DOI Listing
September 2018

[Spatial Distribution of Soil Particles and Heavy Metals Under Different Psammophilic Shrubs in the Ulan Buh Desert].

Huan Jing Ke Xue 2017 Nov;38(11):4809-4818

Taishan Foresty Ecosystem Research Station, Shandong Provincial Key Laboratory of Soil Erosion and Ecological Restoration, College of Forestry, Shandong Agricultural University, Taian 271018, China.

Aeolian sandy soil was collected at depths of 0 to 100 cm at intervals of 0-10, > 10-20, > 20-30, > 30-40, > 40-60, > 60-80, and > 80-100 cm from under the cover of psammophilous shrubs and and from a mobile dune in the Ulan Buh Desert. Multi-fractal theory was used to determine the soil particle size distribution (PSD), which measured the fractions of (Mn), (Zn), (Cr), (Cu), (Pb), (Ni), (As), and (Cd), as well as their distribution status in the laboratory and analyzed correlations between the PSD and heavy metal content to identify the relationship between soil structure and heavy metal pollution. This result shows that a range of different types of shrubs can increase the distribution of fine soil particles, and specifically and can increase range, heterogeneity and degree of dispersion of the PSD. The heavy metal content in the soil under the different shrubs is ranked as (Mn) > (Zn) > (Cr) > (Cu) > (Pb) > (Ni) > (As) > (Cd). In the first 10 cm of the soil under the shrubs, the concentration of all heavy metals are higher than samples from the mobile dune except for Cr and Cu. Average value of (Mn) and (Cd) is 354.899 and 1.429 mg·kg under and respectively. Concentrations of Mn and Cd under and shrubs are significantly higher than those of mobile dune (<0.05), and the concentration of both is significantly various between each soil layers (<0.05). The highest value for (Mn) is in soil layer between 30 and 40 cm below the surface and the highest for (Cd) appears between 10 and 20 cm below the surface. The average (Pb) under is 13.556 mg·kg which is the highest among all the shrubs, exceeding 3.24% under and higher than samples from mobile dune. However, average (As) under is exceeded 0.37% under but still higher than samples from mobile dune. The grain size of (silt) and (medium coarse sand) are determining factors for heavy metal concentrations in fine and coarse particles of aeolian sandy soil. Heavy metal measurements all positively correlate with (silt) and (clay), however, they negatively correlate with (medium coarse sand), except for (Cr). The correlation coefficients between heavy metal contents and the multifractal parameters are similar for (clay), (silt), and (medium coarse sand). Therefore, multifractal parameters can describe the correlation between heavy metals and their determinant soil grain size. This study shows that, and can entrap and deposit fine particles from the sand flow to surface soil layers around shrubs and improve the condition of PSDs for different soil layers with respect to heavy metals. Hence, in aeolian sandy soil these concentrations are higher than for mobile dunes after planting and . Therefore both should be used to promote good soil structure of particular areas to entrap and in-situ control heavy metals to decrease heavy metal pollution in downwind cities.
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http://dx.doi.org/10.13227/j.hjkx.201704135DOI Listing
November 2017

Effects of Periodically Repeated Heat Events on Reproduction and Ovary Development of Agasicles hygrophila (Coleoptera: Chrysomelidae).

J Econ Entomol 2016 08 6;109(4):1586-94. Epub 2016 Jun 6.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China Qingdao Agricultural University, Qingdao, Shandong Province, China.

Insect development occurs within a specific temperature range. Constant temperature studies may produce misleading information on the eco-physiological impacts of temperature on the population dynamics of an insect species, as in most natural environments, temperature usually undergoes daily variation. In China, field surveys showed that the decline in the Agasicles hygrophila (Selman & Vogt) (Coleoptera: Chrysomelidae) population from early August to late September in summer resulted in difficulties in effectively controlling the population of Alternanthera philoxeroides (Mart.) Griseb (Amaranthaceae). Previous studies have largely ignored more natural, fluctuating conditions. In our study, we first investigated the impacts of different temperature conditions (25°C constant temperature for 20 h with a 4-h period of a high temperature of either 30°C, 33°C, 36°C, or 39°C) on adult reproduction and longevity, egg development time, egg hatch rate, female ovarian development, and oogenesis of A. hygrophila. Our results indicated that high temperatures of 30°C and 33°C did not affect the female ovarian development and oogenesis of A. hygrophila Contrarily, high temperatures of 36°C and 39°C negatively affected the population development of A. hygrophila. At 36°C and 39°C, the egg hatch rates were very low, and the egg development times significantly lengthened. The frequency of abnormal ovaries significantly rose at 39°C. We concluded that the decline in the A. hygrophila population during August and September may be related to the extreme high temperatures that frequently occur in summer. These results help provide a better understanding of A. hygrophila population dynamics under natural conditions.
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http://dx.doi.org/10.1093/jee/tow093DOI Listing
August 2016

Distribution of the Multidrug Resistance Gene cfr in Staphylococcus Isolates from Pigs, Workers, and the Environment of a Hog Market and a Slaughterhouse in Guangzhou, China.

Foodborne Pathog Dis 2015 Jul 14;12(7):598-605. Epub 2015 May 14.

1 College of Veterinary Medicine, South China Agricultural University , Guangzhou, China .

Bacteria harboring cfr, a multidrug resistance gene, have high prevalence in livestock in China and might be transmitted to humans through direct contact or via contaminated food products. To better understand the epidemiology of cfr producers in the food chain, the prevalence and genetic analysis of Staphylococcus isolates recovered from pigs, workers, and meat-handling facilities (a slaughterhouse and a hog market in Guangzhou, China) were examined. Twenty (4.5%) cfr-positive Staphylococcus isolates (18 Staphylococcus simulans, 1 S. cohnii, and 1 S. aureus) were derived from pigs (16/312), the environment (2/52), and workers (2/80). SmaI pulsed-field gel electrophoresis of 26 staphylococcal strains (22 S. simulans and 4 S. cohnii), including previously reported cfr-carrying staphylococci of animal food origin, exhibited 19 major pulsed-field gel electrophoresis patterns (A-S). Clonal spread of cfr-carrying staphylococci among pigs, workers, and meat products was detected. The genetic contexts of cfr in plasmids (pHNKF3, pHNZT2, and pHNCR35) obtained from S. simulans of swine or human origin were similar to that of Staphylococcus species isolated from human clinics and animal-derived food. The cfr-carrying S. aureus strain isolated from floor swabs of the hog market was spa-type t889 and belonged to the ST9 clonal lineage. In summary, both clonal spread and horizontal transmission via mobile elements contributed to cfr dissemination among staphylococcal isolates obtained from different sources. To monitor potential outbreaks of cfr-positive bacteria, continued surveillance of this gene in animals at slaughter and in animal-derived food is warranted.
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http://dx.doi.org/10.1089/fpd.2014.1891DOI Listing
July 2015

Trade-offs between survival, longevity, and reproduction, and variation of survival tolerance in Mediterranean Bemisia tabaci after temperature stress.

J Insect Sci 2014 ;14:124

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100081, China Center for Management of Invasive Alien Species, Ministry of Agriculture, Beijing 100081, China.

The invasive Mediterranean Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) has emerged as one of the most common agricultural pests in the world. In the present study, we examined the cross-tolerance, fitness costs, and benefits of thermal tolerance and the variation in the responses of life history traits after heat-shock selection. The results showed that survival and longevity of Mediterranean B. tabaci were decreased significantly after direct or cross temperature stress and that the number of eggs per female was not reduced significantly. Furthermore, heat-shock selection dramatically increased the survival of Mediterranean B. tabaci within two generations, and it did not significantly affect the egg number per female within five generations. These results indicated that there was a trade-off between survival, longevity, and reproduction in Mediterranean B. tabaci after temperature stress. The improvement in reproduction was costly in terms of decreased survival and longevity, and there was a fitness consequence to temperature stress. In addition, heat tolerance in Mediterranean B. tabaci increased substantially after selection by heat shock, indicating a considerable variation for survival tolerance in this species. This information could help us better understand the thermal biology of Mediterranean B. tabaci within the context of climate change.
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http://dx.doi.org/10.1093/jis/14.1.124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4222314PMC
February 2016

Increased survival and prolonged longevity mainly contribute to the temperature-adaptive evolutionary strategy in invasive Bemisia tabaci (Hemiptera: Aleyrodidae) Middle East Asia Minor 1.

J Insect Sci 2014 Oct 15;14:143. Epub 2014 Oct 15.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100193, China Center for Management of Invasive Alien Species, Ministry of Agriculture, Beijing 100193, China.

With increasing global climate change, analyses of stress-inducing conditions have important significance in ecological adaptation and the biological distribution of species. To reveal the difference in temperature-adaptive strategy between Turpan and Beijing populations of Bemisia tabaci (Gennadius) Middle East Asia Minor 1 (MEAM1) under high-temperature stress conditions, we compared thermal tolerance and life history traits between Beijing and Turpan populations of MEAM1 after exposure to different heat shock treatments for different times. The experimental design reflected the nature of heat stress conditions suffered by MEAM1. The results showed that eggs, red-eyed pupae, and adults of the Turpan population were more heat tolerant than those of the Beijing population under the same stress conditions. Additionally, it was found that longevity and F1 adult survival rate were significantly higher in the Turpan population than in the Beijing population after heat shock stress, but egg number and F1 female ratio were not significantly different between Turpan population and Beijing population. Overall, it was suggested that heat tolerance and longevity traits were the most relevant for climate characteristics and not reproductive traits, and improved heat tolerance and prolonged longevity were important adaptive strategies that helped MEAM1 to survive in harsh high-temperature conditions such as Turpan arid desert climate. The present results provided further insight into the modes of heat tolerance and the ways in which survival and longevity traits respond to environmental selection pressures.
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http://dx.doi.org/10.1093/jisesa/ieu005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443472PMC
October 2014

Effect of photoperiod on developmental fitness in Ophraella communa (Coleoptera: Chrysomelidae).

Environ Entomol 2014 Oct 8;43(5):1435-42. Epub 2014 Sep 8.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Ophraella communa LeSage (Coleoptera: Chrysomelidae) is an important biological control agent of the common ragweed, Ambrosia artemisiifolia L., in China. Development and fecundity of O. communa, and hatch rate of progeny eggs were studied at five photoperiods (8:16, 10:14, 12:12, 14:10, and 16:8 [L:D] h). The highest survival rate of eggs was 92% at the photoperiod of 16:8 (L:D) h, and those of both larvae and pupae were observed at the photoperiod of 14:10 (L:D) h (85 and 96%). The shortest developmental durations of larvae and pupae were observed at photoperiods of 14:10 and 16:8 (L:D) h. Fecundity was 1,159-1,976 eggs per female from photoperiods of 8:16 to 16:8 (L:D) h. The hatch rates of progeny eggs were 67-92% from photoperiods of 8:16 to 16:8 (L:D) h, and photoperiods did not affect developmental duration of progeny eggs. The intrinsic rate for increase (r), the net reproductive rate (r0), and the finite rate of increase (λ) reached the maximum values at 16:8 (L:D) h (0.2219 d(-1), 721 hatched eggs and 1.2484 d(-1), respectively) and 14:10 (L:D) h (0.2133 d(-1), 605.6 hatched eggs and 1.2378 d(-1), respectively). Their minimum values were observed at the photoperiod of 8:16 (L:D) h, which were 0.1731 d(-1), 212.2 hatched eggs and 0.1890 d(-1), respectively. The shortest T value was 29.7 d at a photoperiod of 16:8 (L:D) h and the longest was 31.4 d at a photoperiod of 12:12 (L:D) h. Our study shows that O. communa could survive and reproduce successfully at different photoperiods, thus may expand its distribution to regions with different photoperiods.
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http://dx.doi.org/10.1603/EN14003DOI Listing
October 2014

Cold temperatures increase cold hardiness in the next generation Ophraella communa beetles.

PLoS One 2013 30;8(9):e74760. Epub 2013 Sep 30.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

The leaf beetle, Ophraella communa, has been introduced to control the spread of the common ragweed, Ambrosia artemisiifolia, in China. We hypothesized that the beetle, to be able to track host-range expansion into colder climates, can phenotypically adapt to cold temperatures across generations. Therefore, we questioned whether parental experience of colder temperatures increases cold tolerance of the progeny. Specifically, we studied the demography, including development, fecundity, and survival, as well as physiological traits, including supercooling point (SCP), water content, and glycerol content of O. communa progeny whose parents were maintained at different temperature regimes. Overall, the entire immature stage decreased survival of about 0.2%-4.2% when parents experienced cold temperatures compared to control individuals obtained from parents raised at room temperature. However, intrinsic capacity for increase (r), net reproductive rate (R 0) and finite rate of increase (λ) of progeny O. communa were maximum when parents experienced cold temperatures. Glycerol contents of both female and male in progeny was significantly higher when maternal and paternal adults were cold acclimated as compared to other treatments. This resulted in the supercooling point of the progeny adults being significantly lower compared to beetles emerging from parents that experienced room temperatures. These results suggest that cold hardiness of O. communa can be promoted by cold acclimation in previous generation, and it might counter-balance reduced survival in the next generation, especially when insects are tracking their host-plants into colder climates.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074760PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787003PMC
May 2014

Anticoagulant effect of Huisheng Oral Solution in a rat model of thrombosis.

Indian J Pharmacol 2013 Jul-Aug;45(4):359-64

Department of Critical Care Medicine, First Affiliated Hospital of Chinese PLA General Hospital, Beijing-100 048, China.

Objective: To investigate whether Huisheng Oral Solution has an anticoagulant effect in a rat model of thrombosis.

Materials And Methods: A total of 40 male SD rats were equally and randomly divided into four groups: blank group, model group, and two treatment groups (A and B). Rats were subcutaneously injected with carrageenan to induce thrombosis. Rats in the treatment group A were intragastrically administered with Huisheng Oral Solution at a dose of 2 ml/100 g body weight (once per 8 hours), 72 hours after carrageenan injection, while those in the treatment group B were administered with Huisheng Oral Solution both 72 hours before and after induction of thrombosis. Blood samples were collected 24, 48, and 72 hours after carrageenan injection for measurements of prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio (INR), fibrinogen (FIB), prothrombin activity (PTA), platelets (PLT), fibrin degradation products (FDPs), and D-dimer. Lung, liver, and mesentery samples were taken 72 hours after carrageenan injection for histopathological analysis. The numbers of microthrombi in sections of different tissue samples were counted under a microscope. Blood parameters among each group were compared using the Welch test, the Kruskal-Wallis test, or the SNK test after testing for normality, while the number of microthrombi was compared using the Bonferroni test.

Results: Compared to those in the model group, PT, APTT, and INR were significantly prolonged or increased while FIB was significantly reduced at the majority of time points in the two treatment groups (P < 0.05 for all). The levels of FDPs and D-dimer and PLT counts at the majority of time points were significantly lower (P < 0.05 for all), and the numbers of microthrombi in lung, liver, and mesentery samples were significantly decreased (P < 0.05 for all) in the two treatment groups. The above parameters at the majority of time points showed no significant differences between the two treatment groups.

Conclusions: Huisheng Oral Solution can significantly improve coagulation parameters, fibrinolysis parameters, and PLT count, and reduce blood hypercoagulability and microthrombosis, suggesting that Huisheng Oral Solution has an anticoagulant effect in a rat model of thrombosis.
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http://dx.doi.org/10.4103/0253-7613.115018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757604PMC
March 2014

Multiple generation effects of high temperature on the development and fecundity of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) biotype B.

Insect Sci 2013 Aug 5;20(4):541-9. Epub 2012 Oct 5.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Insects are ectotherms and their ability to resist temperature stress is limited. The immediate effects of sub-lethal heat stress on insects are well documented, but longer-term effects of such stresses are rarely reported. In this study, survival, development and reproduction of the whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) biotype B, were compared over five consecutive generations at 27, 31 and 35 °C and for one generation at 37 °C. Both temperature and generation significantly affected the fitness of the whitefly. These impacts were more dramatic with increasing generations and temperatures. Among the experimental temperatures, the most favorable for development and reproduction were 27 °C and 31 °C. At 27 °C, survival, development and fecundity were all stable over these five generations. At 31 °C, immature survival rate was the highest in the fifth generation, but female fecundities decreased in the fourth and fifth generations. At 35 °C, egg hatching rate, immature survival rate and female fecundity decreased significantly in the fourth and fifth generations. At 37 °C, survival of B. tabaci was not adversely affected, but female fecundity at 37 °C was less than 10% of that at 27 °C or 31 °C. These results demonstrate that the lethal high temperature for B. tabaci is over 37 °C, and the whitefly population continued expanding in the five generations at 35 °C. The ability of B. tabaci biotype B to survive high temperature stress will play an important role in its population extension under global warming.
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http://dx.doi.org/10.1111/j.1744-7917.2012.01546.xDOI Listing
August 2013

Effects of high-gossypol cotton on the development and reproduction of Bemisia tabaci (Hemiptera: Aleyrodidae) MEAM1 cryptic species.

J Econ Entomol 2013 Jun;106(3):1379-85

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, #12, Zhong-Guan-Cun Nan-Da-Jie, Haidian, Beijing 100081, China.

Use of plant secondary metabolic compounds is an important method for insect pest control. In this study, the survival, development, and reproduction of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) MEAM1 cryptic species were compared over two consecutive generations on three cotton cultivars of different gossypol levels. Both cotton cultivar and generation significantly affected the fitness of the whitefly. In both generations, the immature development times on the low-gossypol cultivar ZMS13 were significantly longer than those on the high-gossypol cultivar M9101 or medium-gossypol cultivar HZ401. The female fecundity and rate of population increase of the whitefly ranked in the following order: ZMS13 > HZ401 > M9101. On each cultivar, the immature development time was shorter and the immature survival rate was higher in the second generation than those in the first generation. Rate of increase was also higher in the second generation. These results demonstrated that the fitness of B. tabaci MEAM1 cryptic species on the low-gossypol cotton cultivar ZMS13 was higher than that on the medium- or high-gossypol cultivar. The comparison of the life histories of B. tabaci MEAM1 cryptic species on different cotton varieties is important for the development of an integrated pest management program of the whitefly by using plant secondary metabolic compounds.
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http://dx.doi.org/10.1603/ec12401DOI Listing
June 2013

5-Hydroxytryptamine levels in the pulmonary arterioles of broilers with induced pulmonary hypertension and its relationship to pulmonary vascular remodelling.

Avian Pathol 2013 Aug 19;42(4):335-41. Epub 2013 Jun 19.

College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

This experiment was performed to explore the relationship between 5-hydroxytryptamine (5-HT) levels in pulmonary arterioles and in pulmonary vascular remodelling in broilers. Pulmonary arterial hypertension was induced by injecting cellulose microparticles intravenously. Pulmonary hypertension syndrome (PHS) morbidity, right ventricle/total ventricle weight ratio (RV/TV), packed cell volume (PCV), haemoglobin concentration (HB), vessel wall area to vessel total area ratio (WA/TA) and mean tunica media thickness in pulmonary arterioles (mMTPA) were measured. Proliferating cell nuclear antigen (PCNA), argyrophilic nucleolar organizer region proteins (Ag-NORs) and 5-HT content in pulmonary arterioles were determined. The results showed that injecting cellulose microparticles intravenously in broilers could successfully increase the PHS morbidity, significantly elevate RV/TV, PCV and HB, significantly increase mMTPA and WA/TA, and significantly increase the argyrophilic particles in smooth muscle cell nucleoli, PCNA-positive cells in the medial layer, and the 5-HT content in pulmonary arterioles. Correlation analysis showed that the level of 5-HT was strongly positively correlated with PCNA and Ag-NORs. The results indicated that the increase of 5-HT in the tunica media could possibly promote the proliferation of smooth muscle cells in pulmonary arterioles and thus the occurrence of pulmonary vascular remodelling.
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http://dx.doi.org/10.1080/03079457.2013.804903DOI Listing
August 2013

Multi-generation life tables of Bemisia tabaci (Gennadius) biotype B (Hemiptera: Aleyrodidae) under high-temperature stress.

Environ Entomol 2012 Dec;41(6):1672-9

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, #12, Zhong-Guan-Cun Nan-Da-Jie, Haidian, Beijing 100081, China.

Much attention has been focused on insects' ability to survive long-term high-temperature stress and on their resulting population distributions under global warming. In this study, life tables of the whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) biotype B, were collected over five consecutive generations at 27, 31, and 35°C and for one generation at 37°C. At 37°C, the intrinsic rate of increase (r) and the net reproductive rate (r(0)) of the whitefly were 0.0383 d(-1) and 2.8 offspring, respectively. These results demonstrate that the lethal high temperature for B. tabaci is >37°C. At 27°C, neither r(0) nor r decreased over the generations studied. However, both of these values decreased over time at 31 and 35°C, and the decrease was more evident at 35°C. Our results on the ability of B. tabaci biotype B to survive long-term high-temperature stress are important for understanding its population distribution under global warming.
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http://dx.doi.org/10.1603/EN12108DOI Listing
December 2012

[Sedation with stimulative circadian rhythm in mechanically ventilation patients in intensive care unit].

Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 2012 Jul;24(7):402-6

Department of Intensive Care Unit, First Affiliated Hospital, General Hospital of PLA, Beijing, China.

Objective: To sedate the mechanically ventilation patients in intensive care unit (ICU) with stimulative circadian rhythm, and evaluate whether the protocol has advantages in recovering natural circadian rhythm, duration of mechanical ventilation, and length of ICU stay after weaning of sedation.

Methods: A prospective random control trial was conducted. One hundred and twenty ventilated patients in ICU were randomly assigned to four groups: circadian rhythm (CR), daily interruption (DI), continuous sedation (CS) or demand sedation (DS) group, each n = 30. Given more complications, DS group was deleted after recruiting 10 cases and 90 patients were admitted ultimately. Patients' age, gender, body weight, acute physiology and chronic health evaluation II (APACHE II) scores, sedatives dosages, daily arousal time, duration of mechanical ventilation, length of ICU stay, complications (ventilator-associated pneumonia, barotrauma with intrathoracic drain tube) and untoward reactions (accidental extubation, reintubation, tracheotomy, death) were recorded, the biochemical indicators were determined, as well as number of nurses on duty at 10:00 and 22:00.

Results: The patients' sex ratio, age, body weight, APACHEII scores, duration of mechanical ventilation, length of ICU stay showed no difference among CR, DI and CS groups. The total sedatives dosages (mg: 5466.7 ± 620.4) and average sedatives dosages [mg×h(-1) ×kg(-1): 2.19 ± 0.61] in CS group were significantly higher than those in CR group (4344.5 ± 816.0, 1.00 ± 0.51) and DI group (4154.3 ± 649.4, 1.23 ± 0.62, all P < 0.01), and there was no difference between CR group and DI group. Daily arousal time in the CR group (hours: 4.40 ± 1.30) was significantly lengthened compared with that in DI group (0.59 ± 0.26) and CS group (0.15 ± 0.02, both P < 0.05). The complications showed no differences in each group, but incidences of the untoward reactions in DI group (2 cases) were significantly increased compared with that in CR group (1 case) and CS group (0 case, P = 0.0477). After weaning of sedation, patients with normal circadian rhythm were significantly more in CR group than that in CS group (19 vs. 9, P = 0.0339). Among CR group, DI group and CS group, there were significant differences in the numbers of nurses on duty in the daytime (1.65, 1.41, 1.14, all P < 0.01), but there was no difference in the night. The biochemistry index showed no difference in each group.

Conclusions: It demonstrated that sedation with stimulative circadian rhythm be helpful to create circadian rhythm after weaning of sedation. While complications and untoward reactions did not increase, as well as duration of mechanical ventilation and length of ICU stay. Therefore, the clinical applicability of this sedative strategy was highlighted.
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July 2012

Responses of three successive generations of beet armyworm, Spodoptera exigua, fed exclusively on different levels of gossypol in cotton leaves.

J Insect Sci 2010 ;10:165

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.

The beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae), is an important pest of numerous crops, and it causes economic damage in China. Use of secondary metabolic compounds in plants is an important method used to control this insect as a part of integrated pest management. In this study the growth, development, and food utilization of three successive generations of S. exigua fed on three cotton gossypol cultivars were examined. Significantly longer larval life-spans were observed in S. exigua fed on high gossypol cultivar M9101 compared with those fed on two low gossypol cultivars, ZMS13 and HZ401. The pupal weight of the first generation was significantly lower than that of the latter two generations fed on ZMS13 group. Significantly lower fecundity was observed in the second and third generations of S. exigua fed on M9101 compared with S. exigua fed on ZMS13 and HZ401. The efficiency of conversion was significantly higher in the first and third generations fed on HZ401 compared with those fed on ZMS13 and M9101. A significantly lower relative growth rate was observed in the three successive generations fed on M9101 compared with those fed on ZMS13 and HZ401. Cotton cultivars significantly affected the growth, development, and food utilization indices of S. exigua, except for frass and approximate digestibility. Development of S. exigua was significantly affected by relative consumption rate and efficiency of conversion of ingested food, but not by relative growth rate or approximate digestibility, suggesting that diet-utilization efficiency was different based on food quality and generation. Measuring the development and food utilization of S. exigua at the individual and population levels over more than one generation provided more meaningful predictions of long-term population dynamics.
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http://dx.doi.org/10.1673/031.010.14125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016864PMC
February 2011

Effects of temperature on survival, development, longevity, and fecundity of Ophraella communa (Coleoptera: Chrysomelidae), a potential biological control agent against Ambrosia artemisiifolia (Asterales: Asteraceae).

Environ Entomol 2010 Jun;39(3):1021-7

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Ophraella communa (Coleoptera: Chrysomelidae) is a leaf beetle that is unintentionally introduced in China. It is a potential biological control agent against common ragweed, Ambrosia artemisiifolia (Asterales: Asteraceae). The effects of temperature on the development and fecundity of O. communa were studied at eight constant temperature regimens (15, 20, 22, 25, 28, 30, 32, 36 degrees C) in the laboratory. The results showed that the developmental periods for egg, larva, pupa, and entire immature stages decreased in response to the increasing temperature, with the exception of 30 degrees C. The survival rates at different developmental stages were higher at 25 and 28 degrees C than at other temperatures. Ovipositional period and longevity of female shortened with the increasing temperature. The highest fecundity of female was observed to be 2,712.3 eggs/female at 28 degrees C. Life table of O. communa was constructed based on the data at 20-32 degrees C. The innate capacity for increase (r(m)), the net reproductive rate (R(0)), and the finite rate of increase (lambda) reached the maximum at 28 degrees C, with values of 0.247, 1,773.0, and 1.280, respectively. The shortest period of a generation (T) was 24.6 d at 32 degrees C, whereas the longest T value was recorded as 79.3 d at 20 degrees C. These results offer valuable insight on the establishment potential of O. communa in new environments with diverse temperature regimens and on its mass-rearing techniques in laboratory.
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http://dx.doi.org/10.1603/EN09176DOI Listing
June 2010

[Effects of various planting densities on dynamic growth rhythm and root yield of Isatis indigotica].

Zhongguo Zhong Yao Za Zhi 2008 Nov;33(22):2599-602

Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.

Objective: To study the effects of various planting densities on dynamic growth and root yield of Isatis indigotica.

Method: The planting samples were collected to measure the growth period of each organ.

Result And Conclusion: Under different planting densities, both main root length and breadth exhibited a trend of "fast-slow" by stages. However, the number of individual plant leaves were showed a trend of "slow-fast" on growth period. Meanwhile, the leaf length and breadth were exhibited a trend of "increase-decrease". The increase of dry leaf, dry root and whole plant dry matter was faster during the period of 65-76 days after seeding. The differences of root and leaf yields under various densities were significant. Planting densities has a great effect on yield of root. It must be shown that there was a positive development between the individual plant and colony. According to the dry matter of root and leaf, treatment B (i.e., 7 cm x 25 cm) was good choice.
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November 2008

[Comprehensive assessment on control measures of Ambrosia artemisiifolia L].

Ying Yong Sheng Tai Xue Bao 2008 Sep;19(9):1917-24

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China.

Three control measures of Ambrosia artemisiifolia, including biological control, chemical control, and CK without any treatment, were evaluated by analytic hierarchy process (AHP). Corresponding contributions of the three control measures to comprehensive profit (CP) and comprehensive cost (CC) were calculated and ranked, which were regarded as the assessment criteria of the control measures. The results showed that among the three control measures, biological control had the highest CP and the lowest CC, CK was in adverse; and chemical control was in intervenient. Biological control had the highest ratio of profit to cost, and suggesting that this control measure is an optimal and recommendable measure in controlling A. artemisiifolia.
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September 2008

Reproductive biology of two nontarget insect species, Aphis gossypii (Homoptera: Aphididae) and Orius sauteri (Hemiptera: Anthocoridae), on Bt and non-Bt cotton cultivars.

Environ Entomol 2008 Aug;37(4):1035-42

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, CAAS, Beijing 100094, China.

Transgenic Bt cotton, engineered to continuously produce activated delta-endotoxins of the soil bacteria Bacillus thuringiensis, holds great promise in controlling Helicoverpa armigera and other lepidopteran pests. However, it also may impact the invertebrate community, which needs to be clarified. The effects of Bt cotton on two nontarget insects, Aphis gossypii and Orius sauteri, were assessed under semifield and laboratory conditions. Mean total duration of nymphal stages of A. gossypii was shorter (5.9 versus 6.3 d), and rm was higher (0.418 versus 0.394) on conventional Simian 3 (the most frequently planted non-Bt cotton in northern China) than on Bt transgenic NuCOTN 33B (the first Bt cotton commercially planted in China). Mean duration of fourth-instar O. sauteri was significantly longer on transgenic GK-12 (3.7 d) than on NuCOTN 33B (3.2 d), but no different from Simian 3. Mean total mortality was significantly lower on Simian 3 (3.7%) than on GK-12 (14.8%). During the fourth instar, the predator consumed a significantly higher number of prey on Simian 3 (202.3 prey) than on NuCOTN 33B (159.0), whereas the mean total number of A. gossypii prey consumed during the nymphal stage was significantly higher on Simian 3 (336.8 prey) and GK-12 (330.3 prey) than on NuCOTN 33B (275.7). No detrimental effects were detected on development (nymphs, adults, and progeny eggs), fecundity, longevity, and egg viability of O. sauteri on Bt cotton aphids compared with non-Bt cotton aphids. These results suggest that Bt cotton cultivars GK-12 and NuCOTN 33B have no direct effect on nontargets A. gossypii and O. sauteri. Germplasm divergence may account for the negative effects observed on A. gossypii and O. sauteri when reared on NuCOTN 33B or NuCOTN 33B-fed aphids. The biological meanings of the small difference observed between GK-12 and Simian 3 on survival of O. sauteri will require close monitoring over longer time periods.
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http://dx.doi.org/10.1603/0046-225x(2008)37[1035:rbotni]2.0.co;2DOI Listing
August 2008

Tri-trophic interactions between Bt cotton, the herbivore Aphis gossypii Glover (Homoptera: Aphididae), and the predator Chrysopa pallens (Rambur) (Neuroptera: Chrysopidae).

Environ Entomol 2008 Feb;37(1):263-70

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China.

Tri-trophic impacts of transgenic Bacillus thuringiensis (Bt) cotton GK12 and NuCOTN 99B were studied using a predator, the great lacewing Chrysopa pallens (Rambur), and its prey, the cotton aphid Aphis gossypii Glover, in laboratory feeding experiments. The parental nontransgenic cotton cultivar of GK12 was used as control. The predator was fed with uniform (aphids from a single cultivar) or mixed prey (aphids from the three cotton cultivars provided on alternate days). Mortality and development of the immature stages, pupal body mass, adult sex ratio, fecundity, and egg viability of C. pallens were measured. When fed GK12-originated aphid prey, pupal body mass of C. pallens was significantly higher than that of the control, more females emerged, and these females laid significantly more eggs. Other parameters were not impacted. Females emerging from larvae maintained on NuCOTN 99B-originated prey laid fewer eggs than those maintained on GK12. Other measurements did not differ significantly between the two Bt cotton cultivars. Compared with the control, mixed feeding significantly prolonged pupal development time and increased pupal body mass and percentage of females but did not affect other parameters. These results indicate that C. pallens is sensitive to aphid prey from different cotton cultivars. Transgenic Bt cotton GK12-originated aphid prey has no adverse impact on survival, development, and fecundity of C. pallens. Between the two Bt cotton cultivars, NuCOTN 99B-originated aphid prey provided to C. pallens in the larval stage may lower female fecundity. Mixed feeding of C. pallens with the two Bt cotton-originated prey and non-Bt prey may have some adverse impacts on pupal development.
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http://dx.doi.org/10.1603/0046-225x(2008)37[263:tibbct]2.0.co;2DOI Listing
February 2008

[Effects of crop arrangement patterns on arthropod community structure in transgenic bollworm-resistant cotton fields].

Ying Yong Sheng Tai Xue Bao 2007 Sep;18(9):2061-8

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China.

The study on the arthropod community structures in transgenic bollworm-resistant cotton (cv. Lumianyan 15) fields with different crop arrangement patterns showed that compared with those in mono-cultured cotton plot, there were more species of arthropod community and of phytophagous pest and natural enemy sub-communities as well as more individuals of neutral arthropods in vegetable-, fruit tree-, and peanut-cotton plots. More individuals of phytophagous pests in vegetable- and peanut-cotton plots, and natural enemies in vegetable- and fruit tree-cotton plots were observed. The arthropod community in peanut- and fruit tree-cotton plots had the highest similarity, while that in mono-cultured cotton and vegetable-cotton plots had the lowest one. The Renyi diversity index indicated that compared with mono-cultured cotton plot, vegetable-cotton plot had lower diversities of arthropod community and pest sub-community, while fruit tree- and peanut-cotton plots had higher diversities of arthropod community and natural enemy sub-community, and of arthropod community and pest sub-community diversity, respectively. It was concluded that fruit tree-cotton was a recommendable crop arrangement pattern for transgenic bollworm-resistant cotton.
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September 2007

[Application of ISSR molecular marker in invasive plant species study].

Ying Yong Sheng Tai Xue Bao 2007 Apr;18(4):919-27

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China.

Alien species invasion is one of the most important drivers of worldwide environmental change, which may result in environmental degradation, biodiversity loss, and food and water shortage. It may also increase the possibility and severity of natural disasters, and damage international trade and benefits. In last two decades, DNA-based molecular markers were widely used to detect the genetic diversity of invaded alien species. Inter-simple sequence repeat (ISSR) is a microsatellite-based technique, with the superiorities of simple, quick, reliable, and generating higher levels of DNA polymorphism, and being used as a new molecular marker for genetic study. This paper introduced the principles, characteristics and procedures of ISSR, and summarized its applications in studying the genetic structure, genetic diversity, origin, distribution mode, phylogenesis, and breeding features of invasive plants.
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April 2007

[Genetic variations among populations of Ageratina adenophora from different areas, investigated by inter-simple sequence repeat markers].

Fen Zi Xi Bao Sheng Wu Xue Bao 2007 Feb;40(1):41-8

Institute of Plant Protection,Chinese Academy of Agricultural Sciences, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100094, China.

Crofton weed, Ageratina adenophora,a troublesome weed in the world,is an invasive organism in China. Genetic diversity of thirty-two Chinese populations of A. adenophora from different areas was analyzed with the inter-simple sequence repeat (ISSR) technique. It indicated that its genetic diversity was rich. Nei's gene diversity and Shannon's information index were 0.235 and 0.372 at species level respectively. Genetic variation of Crofton weed mainly existed in the population, and about 34.5% of the total variance was attributable to population divergence and 65.5% to individual differences within populations. The Mantel Z-statistic test showed that the genetic distance between populations generally increased with geographic distance (r=0.542,p< 0.001),which indicated that isolation by distance is one of the blocks of gene flow of A. adenophora. Genetic diversity level of Crofton weed had the trend to decrease along with the increased altitude (r=0.368,P<0.001), and the mean of Nei's gene diversity and Shannon's diversity indices both decreased with the increased altitude.
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February 2007

The promoter analysis of the human C17orf25 gene, a novel chromosome 17p13.3 gene.

Cell Res 2002 Dec;12(5-6):339-52

The State-Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Ln 2200/25, Xie-Tu Road, Shanghai 200032, China.

The human C17orf25 gene (Accession No. AF177342) is one of thirteen genes cloned from a region displaying a high score of loss of heterozygosity within chromosome 17p13.3 in human hepatocellular carcinoma in China. To unveil the underlying mechanisms for the transcription regulation of this gene and understand its implication to the hepatocellular carcinogenesis, we looked into the relevant aspects by both bioinformatic and experimental executions. We found: 1, The abundant expression of the C17orf25 gene was evident in all the cell lines and tissue samples tested, showing little hepatoma-selectivity; 2, Its transcription starts at a single site, locating at -60 from the translation initiation codon; 3, A 58 bp fragment containing the transcription start, extending from -112 to -55, represents the minimal promoter; 4, The consensus sequence within this fragment recognized by SP1 contributes predominantly to the activity of the minimal promoter; 5, The bioinformatic analysis suggests that the C17orf25 gene may encode a protein in the family of the glyoxalase. Our data has provided some deep insight into both function and regulation of the C17orf25 gene in the context of the normal liver and hepatocellular carcinoma.
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http://dx.doi.org/10.1038/sj.cr.7290136DOI Listing
December 2002