Publications by authors named "Jian-Ming Li"

190 Publications

Global Comprehensive Literature Review and Meta-Analysis of Brucella spp. in Swine Based on Publications From 2000 to 2020.

Front Vet Sci 2021 7;8:630960. Epub 2021 May 7.

College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China.

Brucellosis, a zoonotic disease, infects various hosts, including swine and humans. It has reemerged in recent years as a public health concern, and current studies on brucellosis infection in swine have been conducted worldwide. However, no meta-analyses of global brucellosis infection in swine have been published. The aim of this study was to provide an overview of species (spp.) in swine worldwide and the factors associated with its persistence. We searched seven databases for published epidemiological studies on brucellosis in pigs, including the Chinese National Knowledge Infrastructure, Wanfang Data, SpringerLink, ScienceDirect, Web of Science, the VIP Chinese Journal Database and PubMed. We selected 119 articles published from January 1, 2000 to January 3, 2020 for inclusion in the meta-analysis and analyzed the data using a random-effects model. Funnel plots and Egger's test showed significant publication bias in the included studies. The results of the sensitivity analysis showed that our study was relatively stable and reliable. The prevalence of brucellosis in swine was 2.1% (95% CI: 1.6-2.6), of which the highest infection rate, which was found in Europe, was 17.4% (95% CI: 11.1-24.9). The prevalence in feral pigs (15.0%, 95% CI: 8.4-23.2) was higher than that in domestic pigs (1.1%, 95% CI 0.2-2.5). The prevalence in high-income countries (15.7%, 95% CI 8.0-25.3) was significantly higher than that in middle- (0.8%, 95% CI 0.5-1.1), and low-income countries (0.1%, 95% CI 0.0-0.2). The prevalence was highest in finishing pigs at 4.9% (95% CI 0.9-11.0), and lowest among suckling pigs at 0% (95% CI 0.0-0.5). The prevalence in pig herds currently is distributed widely throughout the world. In some countries, swine brucellosis may be a neglected zoonotic disease. We recommend long-term monitoring of the prevalence of brucellosis in domestic and wild pig herds. Attention should also be paid to animal welfare on intensive pig farms; controlling the breeding density may play an important role in reducing the spread of brucellosis among pigs.
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http://dx.doi.org/10.3389/fvets.2021.630960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137890PMC
May 2021

MFGE8 mitigates brain injury in a rat model of SAH by maintaining vascular endothelial integrity via TIGβ5/PI3K/CXCL12 signaling.

Exp Brain Res 2021 May 15. Epub 2021 May 15.

Department of Neurosurgery, The Fifth Affiliated Hospital of Sun Yat-sen University, No. 52 Meihuadong Road, Zhuhai, 519000, Guangdong, China.

Leaked blood components, injured endothelial cells, local inflammatory response and vasospasm may converge to promote microthrombosis following subarachnoid hemorrhage (SAH). Previously, we showed that the milk fat globule-epidermal growth factor 8 (MFGE8) can mitigate SAH-induced microthrombosis. This present study was aimed to explore the molecular pathway participated in MFGE8-dependent protection on vascular endothelium. Immunofluorescence, immunoblot and behavioral tests were used to determine the molecular partner and signaling pathway mediating the effect of MFGE8 in vascular endothelium in rats with experimental SAH and controls, together with the applications of RNA silencing and pharmacological intervention methods. Relative to control, recombinant human MFGE8 (rhMFGE8) treatment increased 5-bromo-2'-deoxyuridine (BrdU) labeled new endothelial cells, reduced TUNUL-positive endothelial cells and elevated the expression of phosphatidylinositol 3-kinase (PI3K) and chemokine (C-X-C motif) ligand 12 (CXCL12), in the brains of SAH rats. These effects were reversed by MFGE8 RNA silencing, as well as following cilengitide and wortmannin intervention. These results suggest that MFGE8 promotes endothelial regeneration and mitigates endothelial DNA damage through the activation of the TIGβ5/PI3K/CXCL12 signaling pathway.
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http://dx.doi.org/10.1007/s00221-021-06111-xDOI Listing
May 2021

Prevalence of coccidia in domestic pigs in China between 1980 and 2019: a systematic review and meta-analysis.

Parasit Vectors 2021 May 10;14(1):248. Epub 2021 May 10.

College of Life Science, Changchun Sci-Tech University, Shuangyang, 130600, Jilin, People's Republic of China.

Background: Swine coccidiosis, a protozoan disease caused by coccidia, can result in diarrhoea and weight loss in piglets and even economic losses in the pig industry. Here, we report the first systematic review and meta-analysis of the prevalence of coccidia (including Eimeria spp. and Cystoisospora suis) in pigs in China.

Methods: Five databases (PubMed, ScienceDirect, Chinese Web of Knowledge, Wanfang, and Chongqing VIP) were searched and 50 studies (46,926 domestic pigs, 22 provinces) ultimately identified pertaining to the prevalence of coccidia infection from 1980 to 2019. We incorporated the effect size using the random-effects model in the "meta" package in R software and conducted univariate and multivariate meta-regression analyses using a mixed-effects model.

Results: The pooled prevalence rate of coccidia in pigs was 21.9%, including the C. suis infection rate of 9.1%. The highest prevalence of coccidia (39.6%) was found in northwest China, and this region also presented the lowest prevalence of C. suis (4.7%). In the subgroup analysis based on sampling year, the highest prevalence of coccidia was detected in 2001 or earlier (32.6%), whereas the lowest rate was found in 2012 or later (14.3%). An opposite trend was observed for C. suis (5.5% in 2000 or earlier vs 14.4% in 2000 or later). The prevalence of coccidia in extensive farming systems (29.5%) was higher than that in intensive farming systems (17.3%). In contrast, the point estimate of C. suis prevalence was lower in the extensive farming systems (5.1%) than in the intensive farming systems (10.0%), but the difference was not significant (P > 0.05). Among the four age categories, the highest total coccidia prevalence (26.2%) was found in finishing pigs, followed by suckling piglets (19.9%), whereas the highest prevalence of C. suis (14.9%) was observed in suckling piglets.

Conclusions: Our findings suggest that coccidia infection in Chinese pigs is common, although the prevalence of C. suis in pigs does not receive sufficient attention. We recommend the rational use of anticoccidial drugs to avoid drug resistance and the development of preventive and control measures for C. suis to reduce the incidence of swine coccidiosis.
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http://dx.doi.org/10.1186/s13071-021-04611-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108339PMC
May 2021

Prevalence of in Pigs in China from 1985 to 2020: A Systematic Review and Meta-Analysis.

Vector Borne Zoonotic Dis 2021 Apr 21. Epub 2021 Apr 21.

Laboratory of Production and Product Application of Sika Deer of Jilin Province, Jilin Agricultural University, Changchun, P.R. China.

is a small gram-negative (G-) microorganism that can be dangerous to human and animals. In this study, we conducted a systematic review and meta-analysis of infection in swine in China. From PubMed, ScienceDirect, Chinese Web of knowledge (CNKI), VIP Chinese journal database, and Wanfang database, we collected a total of 72 publications reported in 1985-2020. The prevalence of was 22.48% in China. In the sampling year subgroup, the prevalence after 2011 was the highest (26.14%). In southern China, the prevalence was 30.97%. By contrast, the prevalence in northern China was only 10.79%. Also the difference was significant ( < 0.05). In the provincial level, Hubei had the highest rate of 36.23%. Boars had a higher prevalence (29.47%). The prevalence of detection in pigs with reproductive disorders (21.86%) was higher than that without reproductive disorders. Among the three age groups, finishing pigs (21.43%) had the highest prevalence. The prevalence in large-scale farmed pigs (28.58%) was the highest in the subgroup of feeding methods. The prevalence in farms was 24.29%, which was the highest in the survey areas. The prevalence in spring was the highest with 40.51%. Other methods had the highest prevalence (39.61%) than enzyme-linked immunosorbent assay (ELISA) and indirect hemagglutination assay. The prevalence of 18.41% was lower than the prevalence of (41.35%). We also analyzed the impact of different climate factor subgroups (rainfall, temperature, and humidity) on the probability of pigs suffering from the disease. The results showed that was widespread in pigs in China. We suggest that we should strengthen the detection of in the semen of breeding pigs and pigs with reproductive disorders, and reasonably control the environment of large-scale pig farms, so as to reduce further infection of in pigs.
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http://dx.doi.org/10.1089/vbz.2020.2694DOI Listing
April 2021

α-Fetoprotein mRNA in situ hybridisation is a highly specific marker of hepatocellular carcinoma: a multi-centre study.

Br J Cancer 2021 Jun 6;124(12):1988-1996. Epub 2021 Apr 6.

Department of Pathology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.

Background: Pathologic diagnosis of hepatocellular carcinoma (HCC) can be challenging in differentiating from benign and non-hepatocytic malignancy lesions. The aim of this study was to investigate the potential utility of α-fetoprotein (AFP) mRNA RNAscope, a sensitive and specific method, in the diagnosis of HCC.

Methods: Three independent retrospective cohorts containing 2216 patients with HCC, benign liver lesions, and non-hepatocytic tumours were examined. AFP was detected using ELISA, IHC (Immunohistochemistry), and RNAscope. Glypican3 (GPC3), hepatocyte paraffin-1 (HepPar-1), and arginase-1 (Arg-1) proteins were detected using IHC.

Results: AFP RNAscope improved the HCC detection sensitivity by 24.7-32.7% compared with IHC. In two surgical cohorts, a panel of AFP RNAscope and GPC3 provided the best diagnostic value in differentiating HCC from benign hepatocytic lesions (AUC = 0.905 and 0.811), and a panel including AFP RNAscope, GPC3, HepPar-1, and Arg-1 yielded the best AUC (0.971 and 0.977) when distinguishing HCC from non-hepatocytic malignancies. The results from the liver biopsy cohort were similar, and additional application of AFP RNAscope improved the sensitivity by 18% when distinguishing HCC from benign hepatocytic lesions.

Conclusions: AFP mRNA detected by RNAscope is highly specific for hepatocytic malignancy and may serve as a novel diagnostic biomarker for HCC.
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http://dx.doi.org/10.1038/s41416-021-01363-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184895PMC
June 2021

Meta-analysis of the prevalence of Echinococcus in dogs in China from 2010 to 2019.

PLoS Negl Trop Dis 2021 Apr 2;15(4):e0009268. Epub 2021 Apr 2.

College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, Jilin Province, People's Republic of China.

Background: Echinococcosis (canine Echinococcus disease) is a neglected tropical disease that causes serious public harm. Dogs, as a terminal host of Echinococcus spp., are a key part of the Echinococcus epidemic. Echinococcosis spreads easily in humans and animals in some areas of China and it is therefore necessary to fully understand the prevalence of Echinococcus spp. in dogs.

Methodology/principal Findings: PubMed, ScienceDirect, Chongqing VIP, China National Knowledge Infrastructure (CNKI), and WanFang databases were searched for relevant articles published in the past 10 years. A final total of 108 studies were included. The overall prevalence of Echinococcus spp. in dogs in China was 7.3%, with the highest point estimate found in sampling year 2015 (8.2%) and publication year 2015 (16.5%). Northwestern China (7.9%) had the highest infection rate in China. Qinghai Province (13.5%) showed the highest prevalence among the 11 provinces we included. We also found that geographical and climatic factors are related to the incidence of canine echinococcosis. We further investigated the source of heterogeneity by analysis of subgroups (sampling district, detection method, dog type, season, parasite species, medication, and study quality level).

Conclusions/significance: Our research indicated that Echinococcus spp. were still prevalent in some areas in China. More localized prevention and control policies should be formulated, including improving drinking water hygiene and strengthening hygiene promotion. We recommend the rational use of anti-Echinococcus drugs. In addition, treatment of livestock offal and feces and improving the welfare of stray dogs may play an important role in reducing canine Echinococcus infections.
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http://dx.doi.org/10.1371/journal.pntd.0009268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8018629PMC
April 2021

Targeting Erbin in B cells for therapy of lung metastasis of colorectal cancer.

Signal Transduct Target Ther 2021 Mar 12;6(1):115. Epub 2021 Mar 12.

Department of Pathology, Soochow University Medical School, Suzhou, China.

The mechanisms and key factors involved in tumor environments for lung metastasis of CRC are still unclear. Here, using clinical samples from lung metastases of CRC patients, we found that intestinal immune network for IgA production was significantly dysregulated in lung metastases of CRC. Single-cell RNA sequencing discovered a subtype of B cells positive for Erbin, one member of the leucine-rich repeat and PDZ domain (LAP) family, was involved in the lung metastases. Erbin deletion in B cells suppressed lung metastasis of CRC in vivo. And, deletion of Erbin in B cells enhanced the killing effects of CD8 T cells on tumor cells. Mechanistically, Erbin knockout attenuated TGFβ-mediated suppression of migration of CXCR5 IgA cells and STAT6-mediated PD1 expression. Our study uncovered a key role of Erbin in regulating PD1 IgA B cells in lung metastasis of CRC. Targeting Erbin as well as combined use of neutralizing B cells and antibodies neutralizing PD1 suppresses lung metastasis of CRC in mice, suggesting the potential option for treatment of lung metastasis of CRC.
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http://dx.doi.org/10.1038/s41392-021-00501-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952714PMC
March 2021

Circadian Clock Disruption Suppresses PDL1 Intraepithelial B Cells in Experimental Colitis and Colitis-Associated Colorectal Cancer.

Cell Mol Gastroenterol Hepatol 2021 Feb 27;12(1):251-276. Epub 2021 Feb 27.

Department of Pathology, Soochow University Medical School, Suzhou, China; Department of Pathology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. Electronic address:

Background & Aims: The circadian clock is crucial for physiological homeostasis including gut homeostasis. Disorder of the circadian clock may contribute to many diseases including inflammatory bowel disease (IBD). However, the role and the mechanisms of circadian clock involvement in IBD still are unclear.

Methods: Disorder of the circadian clock including chronic social jet lag and circadian clock gene deficiency mice (Bmal1, and Per1Per2) were established. Dextran sulfate sodium (DSS) and/or azoxymethane were used to induce mouse models of colitis and its associated colorectal cancer. Flow cytometry, immunohistochemistry, immunofluorescence, Western blot, and reverse-transcription quantitative polymerase chain reaction were used to analyze the characteristics of immune cells and their related molecules.

Results: Mice with disorders of the circadian clock including chronic social jet lag and circadian clock gene deficiency were susceptible to colitis. Functionally, regulatory B (Breg) cells highly expressing Programmed cell death 1 ligand 1 (PDL1) in intestinal intraepithelial lymphocytes (IELs) helped to alleviate the severity of colitis after DSS treatment and was dysregulated in DSS-treated Bmal1 mice. Notably, interleukin 33 in the intestinal microenvironment was key for Bmal1-regulated PDL1 Breg cells and interleukin 33 was a target of Bmal1 transcriptionally. Dysregulated PDL1 B cells induced cell death of activated CD4 T cells in DSS-treated Bmal1 mice. Consequently, circadian clock disorder was characterized as decreased numbers of Breg PDL1 cells in IELs and dysfunction of CD4 T cells promoted colitis-associated colorectal cancer (CRC) in mice. In clinical samples from CRC patients, low expression of Bmal1 gene in paracancerous tissues and center area of tumor was associated closely with a poorer prognosis of CRC patients.

Conclusions: Our study uncovers the importance of the circadian clock regulating PDL1 Breg cells of IELs in IBD and IBD-associated CRC.
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http://dx.doi.org/10.1016/j.jcmgh.2021.02.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141473PMC
February 2021

Prevalence of Bovine Tuberculosis in Yaks Between 1982 and 2020 in Mainland China: A Systematic Review and Meta-Analysis.

Vector Borne Zoonotic Dis 2021 Jun 1;21(6):397-405. Epub 2021 Mar 1.

College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China.

Bovine tuberculosis (bTB) is a major chronic bacterial disease in cattle and is the major economic and animal welfare issue in the world. Although the economic costs and public health safety risks associated with the disease are considerable, the overall epidemiology of the Chinese yak () bTB is unclear. To fully reveal the basic prevalence of yak bTB in different regions of China, we searched five databases including PubMed, Science Direct, CNKI (China National Knowledge Infrastructure), Wanfang and Chongqing VIP. Based on the incidence and prevalence of yak tuberculosis in China from 1982 to 2020, a meta-analysis of yak bTB in China was established for the first time. By formulating the search formula, 97 studies were searched in five databases. According to the established exclusion criteria and excluded comments and repeated and irrelevance research, we finally selected 19 cross-sectional studies, which showed the prevalence of bTB in Chinese yaks. Random-effect meta-regression model analysis showed that the estimated prevalence of 122,729 yaks in China was 1.0 (95% confidence interval [CI]: 0.0-1.0). The regional prevalence varies greatly, northwest China prevalence rate 0. 39% (95% CI: 0.2-0. 64) and southwest China prevalence rate 2.59% (95% CI: 1.94-3.34); in terms of province level, the prevalence was highest in Tibet 2.59% (95% CI: 1.94-3.34), followed by Xinjiang 2.36% (95% CI: 0.86-4.58), and Shanxi has the lowest 0.00% (95% CI: 0.00-0.98). This systematic review and meta-analysis identified the estimated prevalence of bTB in Chinese yaks and estimated the underlying factors associated with bTB, including geographic location, sampling year, age, and TB detection method. Provide evidence to plan corresponding disease control strategies for policymakers and to assess future economic risks accurately.
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http://dx.doi.org/10.1089/vbz.2020.2687DOI Listing
June 2021

Seroprevalence and Risk Factors of the Bluetongue Virus in Cattle in China From 1988 to 2019: A Comprehensive Literature Review and Meta-Analysis.

Front Vet Sci 2020 28;7:550381. Epub 2021 Jan 28.

College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China.

Bluetongue caused by the bluetongue virus (BTV) is a non-contagious and an insect-borne disease mainly affecting domestic and wild ruminants. Bluetongue in cattle is associated with vesicular lesions, weight loss, low milk production, and low reproductive capacity. It should not be ignored as it is associated with large economic losses to the livestock breeding industry in China. Although many studies have investigated bluetongue virus infection in cattle, no nationwide study on the prevalence of bluetongue virus infection in cattle from China has yet been conducted. This meta-analysis aimed to evaluate the seroprevalence and risk factors for bluetongue in cattle. We collected 50 publications from 1988 to 2019 through PubMed, ScienceDirect, Chinese Web of Knowledge (CNKI), VIP Chinese journal database, and Wanfang database. A total of the pooled bluetongue seroprevalence of 12.2% (5,332/87,472) in cattle was tested. The point estimate of bluetongue collected from 2001 to 2011 was 22.5% (95% CI: 1.2-58.9), which was higher than after 2012 (9.9%, 95% CI: 3.3-19.4). The analysis of the feeding model subgroup revealed that the seroprevalence of bluetongue was significantly higher ( < 0.05) among free-range cattle (22.5%; 95% CI: 7.7-42.3) than among cattle from intensive farming systems (1.8%; 95% CI: 0.0-6.7). The seroprevalence of bluetongue in different species showed significant variation ( < 0.05), with the highest seroprevalence of 39.8% (95% CI: 18.7-63.0) in buffalo and the lowest seroprevalence of 4.3% (95% CI: 1.2-9.0) in yak. In the zoogeographical division subgroup, the seroprevalence of bluetongue correlated positively within a certain range with the species distribution of . Our findings suggested that bluetongue was prevalent in cattle in China. In addition, the contact with sheep, other ruminants, or transmission media such as may increase the seroprevalence of bluetongue disease in cattle. It is necessary to carry out continuous monitoring of the bluetongue seroprevalence. Moreover, comprehensive and improved strategies and measures should be implemented to prevent and control the spread of bluetongue.
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http://dx.doi.org/10.3389/fvets.2020.550381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901971PMC
January 2021

[Runoff-sediment relationship and erosion dynamic characteristics for two types of engineering deposits under rainfall condition].

Ying Yong Sheng Tai Xue Bao 2020 Sep;31(9):3141-3153

Shaanxi Key Laboratory of Ecological Restoration in Shanbei Mining Area, Yulin University, Yulin 719000, Shaanxi, China.

The engineering deposits produced by the increasing frequency of production and construction activities are the main source of man-made soil erosion. In this study, we examined the change of runoff-sediment relationship and erosion hydrodynamic characteristics with the engineering deposits of aeolian sandy soil and red soil, based on simulated rainfall experiments with different gravel contents (0, 10%, 20%, 30%) and rainfall intensities (1.0, 1.5, 2.0, 2.5 mm·min). The results showed that the sediment yield rate of the aeolian sandy soil deposits gradually increased with the duration of rainfall. The sediment yield rate of red soil deposits under 1.0 mm·min rainfall intensity increased first and then gradually stabilized. Under other rainfall densities, there was a trend of fluctuation after rapid decline, the greater the rainfall intensity and the smaller the gravel content, the more intense the fluctuation. When the gravel content was 0 and 10%, there were rills erosion on the slope surface of aeolian sandy soil accumulation, and the sediment yield rate of rill development stage was 6.74-57.40 times of that of the sheet erosion stage. The erosion process of red soil deposits could be divided into two stages: the loose particle erosion and the soil-rock erosion stage. The sediment yield rate of the loose particle erosion stage was 1.05-3.49 times that of the soil-rock erosion stage. In general, the sediment yield rate of two soil deposits increased with increasing rainfall intensity. The sediment yield rate fluctuated with the increases of gravel content at 1.0 and 1.5 mm·min, with a decreasing trend under >1.5 mm·min. The sediment yield rate of aeolian sandy soil deposits was 1.45-4.14 times of that of red soil deposits under the same rainfall and gravel content conditions. During the erosion process of aeolian sandy soil deposits, the runoff-sediment relationship changed from low sediment concentration to high sediment concentration, while there was a reverse relationship for red soil deposits. During the high sediment concentration period, the increasing rate of the sediment yield rate of aeolian sandy soil deposits was 1.94-37.60 times of that of red soil deposits. For low sediment concentration period, the decreasing rate of the sediment yield rate of red soil deposits was 1.40-21.30 times of that of aeolian sandy soil deposits. In general, the runoff power was better than the runoff shear force in describing the erosion dyna-mics of these two types of deposits. The critical runoff power increased with increasing gravel content. The critical runoff power of aeolian sandy soil deposits during the rill erosion stage (0.02-0.04 W·m) was two times of that of the sheet erosion stage, while the critical stream power was lower than that of the red soil deposits. These results provide a scientific reference for modelling soil erosion processes for engineering deposits.
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http://dx.doi.org/10.13287/j.1001-9332.202009.013DOI Listing
September 2020

Prevalence of bovine leukemia in 1983-2019 in China: A systematic review and meta-analysis.

Microb Pathog 2021 Jan 6;150:104681. Epub 2020 Dec 6.

Laboratory of Production and Product Application of Sika Deer of Jilin Province, Jilin Agricultural University, Changchun, Jilin Province 130118, PR China. Electronic address:

Bovine leukemia is a chronic, progressive, contagious tumor disease characterized by malignant lymphoid cell hyperplasia and systemic lymphadenopathy, and is caused by bovine leukemia virus (BLV). The disease affects almost all countries and regions where livestock are raised, and may even be a potential zoonotic disease. Monitoring and early prevention of bovine leukemia is very important. Therefore, we conducted this meta-analysis, the first of its type in the country, to estimate the prevalence of bovine leukemia in 1983-2019 in China. We included a total of 35 publications reported in 1983-2019 from the PubMed, ScienceDirect, Chinese Web of Knowledge (CNKI), VIP Chinese, and Wan Fang databases. In those articles, a total of 34,954 cattle had been tested, of which 4701 were positive for BLV infection. The estimated pooled BLV prevalence was 10.0% (4701/34,954). Subgroup analysis showed that there were significant differences for sampling years, detection methods, and age. BLV prevalence was highest in the following subgroups: sampled before 1985 (38.5%, 437/1134), age 3-5 years (22.5%, 231/1044), and detected by PCR (17.9%, 1228/5100). Regarding geographic factors, there were significant differences in the latitude and elevation subgroups. BLV prevalence was lowest in the subgroups of 20-30° latitude (3.3%, 255/5069) 200-1000 m altitude (2.2%, 560/11,990). We also analyzed other subgroups such as region, variety, breeding method, precipitation, humidity, and temperature, however, the differences were not significant. Our research indicated that the BLV was still prevalent in some of areas in China. We recommend strengthening the testing of cattle aged >1 year and using flexible testing methods such as PCR to control the prevalence of bovine leukemia and to prevent persistent infection.
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http://dx.doi.org/10.1016/j.micpath.2020.104681DOI Listing
January 2021

The circular RNA circ-ERBIN promotes growth and metastasis of colorectal cancer by miR-125a-5p and miR-138-5p/4EBP-1 mediated cap-independent HIF-1α translation.

Mol Cancer 2020 11 23;19(1):164. Epub 2020 Nov 23.

Department of Pathology and Pathophysiology, Soochow University Medical School, Suzhou, 215123, People's Republic of China.

Background: Circular RNA (circRNAs) and hypoxia have been found to play the key roles in the pathogenesis and progression of cancer including colorectal cancer (CRC). However, the expressions and functions of the specific circRNAs in regulating hypoxia-involved CRC metastasis, and the circRNAs that are relevant to regulate HIF-1α levels in CRC remain elusive.

Methods: qRT-PCR was used to detect the expression of circRNAs and mRNA in CRC cells and tissues. Fluorescence in situ hybridization (FISH) was used to analyze the location of circ-ERBIN. Function-based experiments were performed using circ-ERBIN overexpression and knockdown cell lines in vitro and in vivo, including CCK8, colony formation, EdU assay, transwell, tumor growth and metastasis models. Mechanistically, luciferase reporter assay, western blots and immunohistochemical stainings were performed.

Results: Circ-Erbin was highly expressed in the CRC cells and Circ-Erbin overexpression facilitated the proliferation, migration and metastasis of CRC in vitro and in vivo. Notably, circ-Erbin overexpression significantly promoted angiogenesis by increasing the expression of hypoxia induced factor (HIF-1α) in CRC. Mechanistically, circ-Erbin accelerated a cap-independent protein translation of HIF-1α in CRC cells as the sponges of miR-125a-5p and miR-138-5p, which synergistically targeted eukaryotic translation initiation factor 4E binding protein 1(4EBP-1).

Conclusions: Our findings uncover a key mechanism for circ-Erbin mediated HIF-1α activation by miR-125a-5p-5p/miR-138-5p/4EBP-1 axis and circ-ERBIN is a potential target for CRC treatment.
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http://dx.doi.org/10.1186/s12943-020-01272-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7682012PMC
November 2020

Absolute Resting N-Ammonia PET Myocardial Blood Flow for Predicting Myocardial Viability and Recovery of Ventricular Function after Coronary Artery Bypass Grafting.

J Nucl Cardiol 2020 Oct 21. Epub 2020 Oct 21.

Nuclear Science and Engineering Institute, University of Missouri-Columbia, Columbia, MO, USA.

Objective: We aimed to evaluate the feasibility of resting myocardial blood flow (rMBF), quantified with dynamic 13 N-Ammonia (NH) PET, for identifying myocardial viability and predicting improvement of left ventricular ejection fraction (LVEF) after coronary artery bypass grafting (CABG).

Methods: Ninety-three patients with coronary artery disease (CAD) and chronic LVEF < 45%, scheduled for CABG, had dynamic NH PET and F-FDG PET imaging. The perfusion/metabolism polar maps were categorized in four patterns: normal (N), mismatch (M1), match (M2) and reverse mismatch (RM). The value of rMBF for identifying viable myocardium (M1, RM) and post CABG improvement of LVEF≥8% was analyzed by receiver operating characteristic (ROC) curves. Correlations of rMBF in segments to ΔLVEF post CABG were verified.

Results: Mean rMBFs were significantly different (N=0.60±0.14; M1=0.44±0.07, M2=0.34±0.08, RM=0.53±0.09 ml/min/g, P<0.001). The optimal rMBF cutoff to identify viable myocardium was 0.42 ml/min/g (sensitivity=88.3%, specificity=82.0%) and 0.43 ml/min/g for predicting improvement of LVEF ≥8% (74.6%, 80.0%). The extent and rMBF of combined M1/RM demonstrated a moderate to high correlation to improved LVEF (r=0.78, 0.71, P<0.001).

Conclusion: Resting MBF, derived by dynamic NH PET, may be positioned as a supplement to F-FDG PET imaging for assessing the presence of viable myocardium and predicting potential improvement of LVEF after CABG.
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http://dx.doi.org/10.1007/s12350-020-02388-7DOI Listing
October 2020

Profiling of Tumor Microenvironment Components Identifies Five Stroma-Related Genes with Prognostic Implications in Colorectal Cancer.

Cancer Biother Radiopharm 2020 Oct 21. Epub 2020 Oct 21.

Department of Pathology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

Tumor microenvironment (TME) significantly affects colorectal cancer (CRC) progression and therapeutic efficacy, particularly the infiltrating stromal components. This study aimed to profile the TME composition of tumor tissue and identify TME-related, especially stroma-related genes having prognosis value in CRC patients. We used ESTIMATE algorithm to assess stromal/immune component and divided 524 CRC cases of public dataset into high- and low-score groups. We analyzed effect of the score on prognosis and extracted the differential expression genes (DEGs) between groups, which were stromal- and/or immune-related genes, and performed prognostic investigation of the DEGs. Higher stromal score was correlated with poor survival, whereas immune score was the opposite. By comparing global gene expression of cases with high vs. low stromal/immune scores, we extracted 474 stroma-related genes, 76 immune-related genes, and 498 intersection genes, which were explored by function enrichment and survival analysis. We identified the expression of five stroma-related genes (including , , , , and ) significantly associated with poorer survival, which were validated in the other two independent CRC cohorts. These results presented a comprehensive understanding of TME components and identified five stroma-related genes that predict poor outcomes in CRC patients.
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http://dx.doi.org/10.1089/cbr.2020.4118DOI Listing
October 2020

Impact of Denervation by Heart Transplantation on Post-operative Atrial Fibrillation Susceptibility.

J Atr Fibrillation 2020 Jun-Jul;13(1):2397. Epub 2020 Jun 30.

Cardiac Arrhythmia Center, Cardiovascular Division, Department of Medicine.

Atrial fibrillation is common following cardiac and non-cardiac thoracic surgery and is associated with poorer outcomes, including: increased risk of stroke, hemodynamic instability, prolonged hospital stay, and increased mortality. Current understanding suggests that post-op atrial fibrillation results from the interplay of local and systemic operative inflammation, increased sympathetic activity, perhaps the release of free radical species in the perioperative period, and the patient's underlying cardiac substrate. Cardiac denervation following orthotopic heart transplant (OHT) using modern bicaval techniques presents a unique opportunity to study the relative contribution of the autonomic nervous system to post-op atrial fibrillation susceptibility. Observational studies show a reduced incidence of post-operative atrial fibrillation following orthotopic heart transplant compared to other cardiac and thoracic surgeries. Moreover, comparison of atrial fibrillation rates with double lung transplant recipients suggests that cardiac denervation has a contribution apart from surgical pulmonary vein isolation alone. This report reviews current concepts of the mechanisms of post-op atrial fibrillation with a focus on the role of the autonomic nervous system, the autonomic regulation of the native heart, and evidence regarding the impact of cardiac denervation following OHT.
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http://dx.doi.org/10.4022/jafib.2397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533138PMC
June 2020

Prevalence of porcine circovirus 2 throughout China in 2015-2019: A systematic review and meta-analysis.

Microb Pathog 2020 Dec 18;149:104490. Epub 2020 Sep 18.

Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education of the People's Republic of China, PR China; Laboratory of Production and Product Application of Sika Deer of Jilin Province, Jilin Agricultural University, Changchun Jilin Province 130118, PR China. Electronic address:

Porcine circovirus type 2 (PCV2) causes infectious diseases in pigs leading to considerable economic losses in the pig industry. To prevent and control PCV2 infections, is important to understand the prevalence and geographical distribution of the virus. We performed the first systematic review and meta-analysis to estimate the prevalence of PCV2 in China. From PubMed, ScienceDirect, Chinese Web of Knowledge, Wanfang, and VIP Chinese Journal, we extracted 53 studies published in China between 2015 and 2019. There were 29,051 samples, 14,230 of which were positive for PCV2. The pooled prevalence of PCV2 was 46.0%, with the highest in Northeastern China (58.1%). The highest prevalence was 86.3% in Xinjiang province. Nursery pigs had the highest prevalence of PCV2 (50.9%), and the serological test detected the highest number of cases (58.5%). PCV2 prevalence was 50.1% in intensive farms and 37.5% in extensive farms. Our findings showed that PCV2 is common throughout China. Effective control measures are necessary to reduce PCV2 infections.
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http://dx.doi.org/10.1016/j.micpath.2020.104490DOI Listing
December 2020

The gut microbiota regulates autism-like behavior by mediating vitamin B homeostasis in EphB6-deficient mice.

Microbiome 2020 08 20;8(1):120. Epub 2020 Aug 20.

Department of Pathology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, People's Republic of China.

Background: Autism spectrum disorder (ASD) is a developmental disorder, and the effective pharmacological treatments for the core autistic symptoms are currently limited. Increasing evidence, particularly that from clinical studies on ASD patients, suggests a functional link between the gut microbiota and the development of ASD. However, the mechanisms linking the gut microbiota with brain dysfunctions (gut-brain axis) in ASD have not yet been full elucidated. Due to its genetic mutations and downregulated expression in patients with ASD, EPHB6, which also plays important roles in gut homeostasis, is generally considered a candidate gene for ASD. Nonetheless, the role and mechanism of EPHB6 in regulating the gut microbiota and the development of ASD are unclear.

Results: Here, we found that the deletion of EphB6 induced autism-like behavior and disturbed the gut microbiota in mice. More importantly, transplantation of the fecal microbiota from EphB6-deficient mice resulted in autism-like behavior in antibiotic-treated C57BL/6J mice, and transplantation of the fecal microbiota from wild-type mice ameliorated the autism-like behavior in EphB6-deficient mice. At the metabolic level, the disturbed gut microbiota in EphB6-deficient mice led to vitamin B and dopamine defects. At the cellular level, the excitation/inhibition (E/I) balance in the medial prefrontal cortex was regulated by gut microbiota-mediated vitamin B in EphB6-deficient mice.

Conclusions: Our study uncovers a key role for the gut microbiota in the regulation of autism-like social behavior by vitamin B, dopamine, and the E/I balance in EphB6-deficient mice, and these findings suggest new strategies for understanding and treating ASD. Video abstract.
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http://dx.doi.org/10.1186/s40168-020-00884-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7441571PMC
August 2020

Preliminary and Comparative Experiment Study Between F-Flurpiridaz and N-NHHO Myocardial Perfusion Imaging With PET/CT in Miniature Pigs.

Mol Imaging 2020 Jan-Dec;19:1536012120947506

Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.

Objectves: To comparatively explore the differences between F-Flurpiridaz and N-NH·HO PET/CT myocardial perfusion imaging in miniature pigs.

Methods: Ten Bama minipigs were divided into normal group and myocardial infarction group. The changes of the ratio of left ventricular myocardium to main organs with time were calculated and the best imaging time was confirmed for F-Flurpiridaz imaging in normal group. The image quality score, summed rest score(SRS), Extend, total perfusion deficit(TPD) and left ventricle ejection fraction(LVEF) were respectively compared for F-Flurpiridaz and N-NH·HO in infarction group.

Results: F-Flurpiridaz was rapid distributed in myocardium, and the background counts of cardiac cavity were very low, and no obvious interference extracardiac radioactivity was observed. The radioactive ratio of the left ventricular myocardium to cardiac blood pool and adjacent liver were high. Compared with N-NH·HO, there were no significant differences in functional parameters, including SRS, Extend, TPD and LVEF.

Conclusion: The results preliminaryly show that F-FIurpiridaz is a promising positron MPI agent with good image quality, ability of accurately evaluating cardiac function, and also convenience for application.
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http://dx.doi.org/10.1177/1536012120947506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7543149PMC
August 2020

Development and validation of the immune signature to predict distant metastasis in patients with nasopharyngeal carcinoma.

J Immunother Cancer 2020 04;8(1)

Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangzhou, Guangdong Province, People's Republic of China

Background: The tumor immune microenvironment has clinicopathological significance in predicting prognosis and therapeutic efficacy. We aimed to develop an immune signature to predict distant metastasis in patients with nasopharyngeal carcinoma (NPC).

Methods: Using multiplexed quantitative fluorescence, we detected 17 immune biomarkers in a primary screening cohort of 54 NPC tissues presenting with/without distant metastasis following radical therapy. The LASSO (least absolute shrinkage and selection operator) logistic regression model used statistically significant survival markers in the training cohort (n=194) to build an immune signature. The prognostic and predictive accuracy of it was validated in an external independent group of 304 patients.

Results: Eight statistically significant markers were identified in the screening cohort. The immune signature consisting of four immune markers (PD-L1+ CD163+, CXCR5, CD117) in intratumor was adopted to classify patients into high and low risk in the training cohort and it showed a high level of reproducibility between different batches of samples (r=0.988 for intratumor; p<0.0001). High-risk patients had shorter distant metastasis-free survival (HR 5.608, 95% CI 2.619 to 12.006; p<0.0001) and progression-free survival (HR 2.798, 95% CI 1.498 to 5.266; p=0·001). The C-indexes which reflected the predictive capacity in training and validation cohort were 0.703 and 0.636, respectively. Low-risk patients benefited from induction chemotherapy plus concurrent chemoradiotherapy (IC+CCRT) (HR 0.355, 95% CI 0.147 to 0.857; p=0·021), while high-risk patients did not (HR 1.329, 95% CI 0.543 to 3.253; p=0·533). To predict the individual risk of distant metastasis, nomograms with the integration of both immune signature and clinicopathological risk factors were developed.

Conclusions: The immune signature provided a reliable estimate of distant metastasis risk in patients with NPC and might be applied to identify the cohort which benefit from IC+CCRT.
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http://dx.doi.org/10.1136/jitc-2019-000205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7204817PMC
April 2020

Prevalence of bovine viral diarrhea virus (BVDV) in yaks between 1987 and 2019 in mainland China: A systematic review and meta-analysis.

Microb Pathog 2020 Jul 6;144:104185. Epub 2020 Apr 6.

College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province, 130118, China; Key Lab of Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin Province, 130118, China. Electronic address:

Background: Bovine viral diarrhea is an infectious disease that causes symptoms such as bovine diarrhea and abortion. It can cause severe losses to the animal husbandry, and the overall epidemic situation of yak's BVDV in China is unclear. Meta-analysis can reveal the basic epidemic situation of BVDV in different yak distribution areas in China, and estimate potentially related factors, to pave the way for clarifying the epidemic situation of yak in the domestic scope.

Methods: We proceeded to a systematic review and meta-analysis of data from papers on the BVDV incidence and prevalence in yaks in China by searching PubMed, ScienceDirect, Chinese Web of Knowledge (CNKI), Wanfang, and Chongqing VIP for publication from 1987 to 2019. We excluded reviews and duplicate studies, 24 studies denouncing the prevalence of BVDV in yak in China were selected upon our inclusion criterion finally. We estimated the pooled prevalence of BVDV infection in yaks by a random-effects model and evaluated its overall infection burden in China.

Findings: In total, the pooled prevalence of BVDV in yaks in China was 36.0% (95% CI 25.6%-46.4%) based on the data obtained from 13,446 yaks, by detecting antigens and antibodies. The highest BVDV positive rate in yak reached 67.5% in Xinjiang province of China. The prevalence in the six provinces of China was validated to be quite variable (24.4%-67.5%) and reached 369% in yaks of northwest China. Besides, the BVDV antigen-positive rate was estimated at 13.8% (95% CI 8.6%-19.0%) based on 5 studies, comparatively, the pooled BVDV antibody-based on 18 studies was about 32.9% (95% CI 24.6%-41.2%) in China.

Interpretation: This systematic review and meta-analysis firstly established an estimated prevalence of BVDV in yaks in China, as a whole, and estimates potential relevant factors, including geographic location, publication year, age, detection methods, etc., Our findings suggest that the scientific community or decision-makers can formulate corresponding prevention and control plans based on potential risk factors.
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http://dx.doi.org/10.1016/j.micpath.2020.104185DOI Listing
July 2020

Significance-based multi-scale method for network community detection and its application in disease-gene prediction.

PLoS One 2020 20;15(3):e0227244. Epub 2020 Mar 20.

School of Computer Science and Engineering, Central South University, Changsha, Hunan, People's Republic of China.

Community detection in complex networks is an important issue in network science. Several statistical measures have been proposed and widely applied to detecting the communities in various complex networks. However, due to the lack of flexibility resolution, some of them have to encounter the resolution limit and thus are not compatible with multi-scale structures of complex networks. In this paper, we investigated a statistical measure of interest for community detection, Significance [Sci. Rep. 3 (2013) 2930], and analyzed its critical behaviors based on the theoretical derivation of critical number of communities and the phase diagram in community-partition transition. It was revealed that Significance exhibits far higher resolution than the traditional Modularity when the intra- and inter-link densities of communities are obviously different. Following the critical analysis, we developed a multi-resolution version of Significance for identifying communities in the multi-scale networks. Experimental tests in several typical networks have been performed and confirmed that the generalized Significance can be competent for the multi-scale communities detection. Moreover, it can effectively relax the first- and second-type resolution limits. Finally, we displayed an important potential application of the multi-scale Significance in computational biology: disease-gene identification, showing that extracting information from the perspective of multi-scale module mining is helpful for disease gene prediction.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0227244PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083276PMC
May 2020

QTc dispersion as a novel marker in identifying patients requiring an epicardial approach for ablation of scar mediated ventricular tachycardia.

Indian Pacing Electrophysiol J 2020 Jul - Aug;20(4):141-146. Epub 2020 Mar 7.

Cardiovascular Division, University of Minnesota Medical School, Minneapolis, MN, USA; Cardiovascular Division, Minneapolis VA Health Care System, Minneapolis, MN, USA. Electronic address:

Introduction: Epicardial exit sites of ventricular tachycardia (VT) are frequently encountered during VT ablation requiring an epicardial ablation approach for successful elimination of VT. We sought to assess the utility of repolarization markers in identifying individuals requiring an epicardial ablation approach in addition to an endocardial approach.

Methods: 32 patients who underwent successful ablation for scar mediated VT were included in the study. Fourteen patients who required a combined endocardial and epicardial VT ablation were defined as epicardial VT group (Epi) whereas 18 patients who were successfully ablated from the endocardium alone constituted the endocardial VT group (Endo). Repolarization markers during sinus rhythm were compared between the two groups.

Results: A higher QTc max and QTc dispersion were seen in the Epi group compared to Endo group (479 ± 34 vs 449 ± 20, p = 0.008 and 63 ± 13 vs 38 ± 8, p = 0.001, respectively). Ts-p and Ts-p/Tp-e were higher in the Epi group (166 ± 23 vs 143 ± 23, p = 0.008 and 1.55 ± 0.26 vs 1.3 ± 0.21, p < 0.005). On multivariate regression, QTc dispersion was an independent predictor of the need for an epicardial approach to ablation. A QTc dispersion more than 51.5 msec identified individuals requiring a combined epicardial and endocardial approach to ablation with a sensitivity of 92.9% and a specificity of 100%.

Conclusions: Patients requiring an epicardial ablation have a higher QTc dispersion. A value greater than 51.5 msec reliably differentiates between the two groups with high sensitivity and specificity.
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http://dx.doi.org/10.1016/j.ipej.2020.03.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7384369PMC
March 2020

Seroprevalence of Toxoplasma gondii in cattle in China from 2010 to 2019: A systematic review and meta-analysis.

Acta Trop 2020 Nov 7;211:105439. Epub 2020 Mar 7.

College of Life Science, Changchun Sci-Tech University, Shuangyang, Jilin Province 130600, PR China. Electronic address:

Toxoplasma gondii can infect almost all warm-blooded animals, including cattle, causing serious disease to animals and humans. Because cattle provide milk and meat for humans, an increasing number of people are raising cattle as a source of income. However, T. gondii may be transmitted to humans through the meat and milk of cattle. Although substantial relevant studies have been performed, overall data on the seroprevalence of T. gondii in cattle is still lacking. In this meta-analysis, 71 relevant studies were included from five databases that investigated the seroprevalence of T. gondii in cattle in China from 2010 to 2019. The overall pooled T. gondii seroprevalence in cattle was 10.1% (4217/39,274) in China. The seroprevalence of T. gondii was highest in southwestern China (21.6%, 727/3117) and lowest in northern China (4.5%, 185/1966). Due to the implementation of disease control measures during 2012 to 2020, the lowest positive rate was seen in the post-2017 subgroup of sampling years (5.8%, 170/3022). In the cattle breeds subgroups, the seroprevalence was highest in yaks (14.3%, 1695/10,777). Cattle aged ≥12 months (9.6%, 1248/12,438) had a higher seroprevalence than those <12 months (6.7%, 226/3132). Female cattle had a slightly higher seroprevalence (12.5%, 793/6670) than male cattle (11.7%, 418/3856). The seroprevalence in summer (11.8%, 517/4744) was the highest of all seasons. The present study shows that T. gondii is common in cattle in China. Therefore, monitoring measures and further research are needed to control T. gondii and improve public health.
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http://dx.doi.org/10.1016/j.actatropica.2020.105439DOI Listing
November 2020

A novel STAT3 inhibitor attenuates angiotensin II-induced abdominal aortic aneurysm progression in mice through modulating vascular inflammation and autophagy.

Cell Death Dis 2020 02 18;11(2):131. Epub 2020 Feb 18.

Department of Cardiovascular Surgery, The Second Xiang-ya Hospital, Central South University, Changsha, Hunan, P.R. China.

Abdominal Aortic aneurysm (AAA) is associated with chronic inflammation, cells apoptosis, and impairment of autophagy. BP-1-102, a novel potent STAT3 inhibitor, has been recently reported to significantly block inflammation-related signaling pathways of JAK2/STAT3 and NF-κB, as well as regulate autophagy. However, its role in vascular inflammation and AAA progression remains to be elucidated. In the present study, the effect and potential mechanisms of BP-1-102 on angiotensin II (AngII) induced AAA in ApoE mice were investigated. AAA was induced in ApoE mice with infusion of AngII for 28 days. BP-1-102 was administrated orally to mice every other day. Mice were sacrificed on day 7, day 14, and day 28 to evaluate the treatment effects. BP-1-102 markedly decreased AAA incidence and aortic diameter, maintained elastin structure and volume, reduced the expression of pro-inflammatory cytokines and MMPs, and inhibited inflammatory cells infiltration. Moreover, BP-1-102 dramatically reduced the expression of JAK2, p-STAT3, p-NF-κB, and Bcl-xL but maintained the expression of LC3B and Beclin in AAA tissues. In vitro, vascular smooth muscle cells (VSMCs) were treated with AngII and/or BP-1-102 at indicated time and concentration. BP-1-102 inhibited AngII-induced JAK2/STAT3 and NF-κB signaling activation and maintained autophagy-related proteins expression in VSMCs. Taken together, our findings suggest that BP-1-102 inhibits vascular inflammation and AAA progression through decreasing JAK2/STAT3 and NF-κB activation and maintaining autophagy.
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http://dx.doi.org/10.1038/s41419-020-2326-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7028955PMC
February 2020

Synaptic development of layer V pyramidal neurons in the prenatal human prefrontal neocortex: a Neurolucida-aided Golgi study.

Neural Regen Res 2020 Aug;15(8):1490-1495

Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha, Hunan Province, China.

The prefrontal neocortex is involved in many high cognitive functions in humans. Deficits in neuronal and neurocircuitry development in this part of the cerebrum have been associated with various neuropsychiatric disorders in adolescents and adults. There are currently little available data regarding prenatal dendrite and spine formation on projecting neurons in the human prefrontal neocortex. Previous studies have demonstrated that Golgi silver staining can identify neurons in the frontal lobe and visual cortex in human embryos. In the present study, five fetal brains, at 19, 20, 26, 35, and 38 gestational weeks, were obtained via the body donation program at Xiangya School of Medicine, Central South University, China. Golgi-stained pyramidal neurons in layer V of Brodmann area 46 in fetuses were quantitatively analyzed using the Neurolucida morphometry system. Results revealed that somal size, total dendritic length, and branching points of these neurons increased from 26 to 38 gestational weeks. There was also a large increase in dendritic spines from 35 to 38 gestational weeks. These findings indicate that, in the human prefrontal neocortex, dendritic growth in layer V pyramidal neurons occurs rapidly during the third trimester of gestation. The use of human fetal brain tissue was approved by the Animal Ethics Committee of Xiangya School of Medicine, Central South University, China (approval No. 2011-045) on April 5, 2011.
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http://dx.doi.org/10.4103/1673-5374.274345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059576PMC
August 2020

The expressions and significance of B7-H3 and CTLA-4 in the clinical stages of non-small-cell lung cancer.

Int J Clin Exp Pathol 2019 1;12(8):3032-3041. Epub 2019 Aug 1.

Department of Respiratory Medicine, The Petroleum Clinical Medical College of Hebei Medical University Langfang, Hebei, China.

The aim of this study was to analyze the expressions and significance of B7-H3 and CTLA-4 in the clinical stages of non-small-cell lung cancer (NSCLC). Seventy patients with NSCLC who underwent surgical resection or biopsy between January 2016 and February 2018 were enrolled. Among them, 30 were cases of paracancerous tissues and were assigned to the control group (CON). These cases were analyzed using immunochemical methods. Of the 70 cases, 48 were of adenocarcinoma, 19 were of squamous cell carcinoma, and 3 were of adenosquamous carcinoma. The expression rates of B7-H3 and CTLA-4 in the observation group (OBS) were 64.2% and 57.1% respectively, and those in the CON group were 6.7% and 0%, respectively (χ=27.988, 28.571, <0.001). The expression levels of B7-H3 and CTLA-4 in patients with poor differentiation, in stages III-IV, or with lymph node metastasis were significantly higher than those in patients with good-to-moderate differentiation, in stages I-II, and without lymph node metastasis (<0.05). There was a positive correlation between the expressions of B7-H3 and CTLA-4 in the OBS group (r=0.74, <0.05). B7-H3 and CTLA-4 are highly expressed and positively correlated with each other in NSCLC patients and are also closely related to clinical stages.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6949705PMC
August 2019

An Overview of Computational Tools of Nucleic Acid Binding Site Prediction for Site-specific Proteins and Nucleases.

Protein Pept Lett 2020 ;27(5):370-384

Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, China.

Understanding the interaction mechanism of proteins and nucleic acids is one of the most fundamental problems for genome editing with engineered nucleases. Due to some limitations of experimental investigations, computational methods have played an important role in obtaining the knowledge of protein-nucleic acid interaction. Over the past few years, dozens of computational tools have been used for identification of nucleic acid binding site for site-specific proteins and design of site-specific nucleases because of their significant advantages in genome editing. Here, we review existing widely-used computational tools for target prediction of site-specific proteins as well as off-target prediction of site-specific nucleases. This article provides a list of on-line prediction tools according to their features followed by the description of computational methods used by these tools, which range from various sequence mapping algorithms (like Bowtie, FetchGWI and BLAST) to different machine learning methods (such as Support Vector Machine, hidden Markov models, Random Forest, elastic network and deep neural networks). We also make suggestions on the further development in improving the accuracy of prediction methods. This survey will provide a reference guide for computational biologists working in the field of genome editing.
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http://dx.doi.org/10.2174/0929866526666191028162302DOI Listing
December 2020

Percutaneous gallbladder drainage as a bridge to concomitant coronary artery bypass grafting and laparoscopic cholecystectomy.

J Card Surg 2020 Feb 15;35(2):441-443. Epub 2019 Nov 15.

Department of Cardiovascular Surgery, The Second Xiang-ya Hospital, Central South University, Changsha, Hunan, China.

A 74-year-old woman with left main and three-vessel coronary artery disease was scheduled for off-pump coronary artery bypass grafting and developed acute severe cholecystitis preoperatively. Percutaneous gallbladder drainage was placed to achieve gallbladder decompression and infection control. Two weeks later, CABG and laparoscopic cholecystectomy were successfully performed at the same time.
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http://dx.doi.org/10.1111/jocs.14340DOI Listing
February 2020