Publications by authors named "Jian-Hong Li"

85 Publications

Magnetic metal-organic framework MIL-100 (Fe)/polyethyleneimine composite as an adsorbent for the magnetic solid-phase extraction of fungicides and their determination using HPLC-UV.

Mikrochim Acta 2021 Jan 7;188(2):33. Epub 2021 Jan 7.

Faculty of Agriculture, Department of Plant Protection, Fayoum University, Fayoum, 63514, Egypt.

[email protected] (Fe)/PEI are used for the first time as an adsorbent material for the extraction of pesticide residues (epoxiconazole, flusilazole, tebuconazole, and triadimefon) from food matrices. The adsorbent proposed ([email protected](Fe)/PEI) was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM), thermogravimetric (TG) analysis, and vibrating sample magnetometer (VSM) techniques to evaluate the properties of the sorbent. Then, the [email protected] (Fe)/PEI was employed for the quantification of the four triazole fungicides in fruits and vegetables (apple, orange, tomato, cabbage, and cucumber) using HPLC-UV for separation and detection. During the extraction process, the main parameters such as amount of adsorbent, extraction time, pH value, ionic strength, eluting solvent, and eluting volume were optimized. Under the optimum conditions, good linearity of this method was observed for all analytes, with correlation coefficients (R) ≥ 0.9908. The limits of detection (LODs) ranged from 0.021-3.04 μg kg. The extraction recoveries of the four triazole fungicides varied from 73.9 to 109.4% with relative standard deviations (RSD) in the range 0.5 to 6.2%. Compared with other MOFs, the modification of [email protected] (Fe) with PEI shows high efficient adsorption due to the combined benefits of MIL-100 (Fe) and PEI. The material is easily synthesized, has good stability, and is of low cost.  Graphical abstract.
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http://dx.doi.org/10.1007/s00604-020-04648-2DOI Listing
January 2021

Magnetic solid-phase extraction based on nano-zeolite imidazolate framework-8-functionalized magnetic graphene oxide for the quantification of residual fungicides in water, honey and fruit juices.

Food Chem 2020 Apr 29;325:126944. Epub 2020 Apr 29.

College of Plant Science and Technology, Department of Plant Protection, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

In this work, zeolite imidazolate framework-8 (ZIF-8) functionalized magnetic graphene oxide ([email protected]/ZIF-8) was successfully synthesized and used as a novel adsorbent in magnetic solid-phase extraction (MSPE) for the determination of four triazole fungicides in water, honey and fruit juices. The main parameters such as extraction time, amount of adsorbent, the pH value of the sample, ionic strength, and desorption solvent which could affect the experiment results were optimization. Under the optimum condition, the obtained linearity of this method ranged from 1 to 1000 µg L for all analytes, with correlation coefficients (R) ≥ 0.9914. Limit of detections (LODs) and limit of qualifications (LOQs) of four triazole fungicides were ranged from 0.014 to 0.109 µg L and from 0.047 to 0.365 µg L, respectively. Based on comparison with outcomes from other studies, [email protected]/ZIF-8-MSPE could provide high performance and achieve satisfied results for the analysis of trace triazole fungicides in complicated matrices.
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http://dx.doi.org/10.1016/j.foodchem.2020.126944DOI Listing
April 2020

Current susceptibilities of brown planthopper Nilaparvata lugens to triflumezopyrim and other frequently used insecticides in China.

Insect Sci 2021 Feb 13;28(1):115-126. Epub 2020 Apr 13.

Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

The brown planthopper is a notorious rice pest in many areas of Asia. The evolution of insecticide resistance in Nilaparvata lugens has become a serious problem in the effective control of this pest in the paddy field. In this article, the current susceptibility of N. lugens field populations to novel mesoionic insecticide triflumezopyrim and major classes of chemical insecticides was determined and compared. The monitoring results indicated that field populations of N. lugens had developed low resistance to triflumezopyrim (resistance ratio, RR: 1.3-7.3-fold) during 2015-2018 in China, and the median lethal concentration values varied from 0.05 to 0.29 mg/L. Additionally, during 2017 to 2018, field populations of N. lugens showed high resistance levels to thiamethoxam (RR: 456.1-1025.6-fold), imidacloprid (RR: 2195.3-6899.0-fold) and buprofezin (RR: 1241.5-4521.7-fold), moderate to high resistance levels to dinotefuran (RR: 97.6-320.1-fold), clothianidin (RR: 69.4-230.1-fold) and isoprocarb (RR: 44.1-108.0-fold), and low to moderate levels of resistance to chlorpyrifos (RR: 12.0-29.7-fold) and nitenpyram (RR: 6.9-24.1-fold). In contrast, N. lugens just showed low resistance to sulfoxaflor (RR: 3.3-8.5-fold) and etofenprox (RR: 5.0-9.1-fold) in the field. Additionally, the P450 gene CYP6ER1 was found to be significantly overexpressed in all five field populations of N. lugens collected in 2018 when compared with a laboratory susceptible strain. Our findings will provide useful information to delay the evolution of insecticide resistance in N. lugens.
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http://dx.doi.org/10.1111/1744-7917.12764DOI Listing
February 2021

The enantioselective effects and potential risks of paclobutrazol residue during cucumber pickling process.

J Hazard Mater 2020 03 23;386:121882. Epub 2019 Dec 23.

College of Plant Science and Technology, Department of Plant Protection, Huazhong Agriculture University, Wuhan, 430070, China. Electronic address:

For decades, pesticides have been widely used for controlling pests and protecting crops around the world, and the food safety issues caused by these compounds have raised widespread concern. However, the different enantioselective behaviors and biological activities of chiral pesticide enantiomers are often ignored. In this work, a novel method was put forward to investigate the enantioselective effects and potential risks of two paclobutrazol enantiomers during cucumber pickling process. The degradation kinetics indicated that when paclobutrazol reside in cucumber and was introduced into the pickling process, the half-time of paclobutrazol isomers were significantly different (9.24 d and 16.6 d), and the conversion phenomenon between the two enantiomers could also be observed. In addition, results from 16S rRNA and ITS sequencing shown that (2R, 3R)-paclobutrazol and (2S, 3S)-paclobutrazol have an obviously enantiomeric effects on microbial community of pickling system and the degradation of paclobutrazol was probably attributed to the presence of Pseudomonas and Serratia. Finally, the microorganisms functions were found to be disrupted under the exposure of (2R, 3R)-paclobutrazol and metabolic function of microorganisms to xenobiotic was inhibited, which might cause potential risks to the quality of preserved foods. In summary, we have devised a method and provided a novel insight into the potential risks of chiral pesticide residues on food safety and human health.
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http://dx.doi.org/10.1016/j.jhazmat.2019.121882DOI Listing
March 2020

['s experience in treating dry eye syndrome with fire needling].

Zhongguo Zhen Jiu 2019 Sep;39(9):983-6

Guangzhou University of CM, Guangzhou 510405, Guangdong Province, China.

Professor - is the representative inheritor of fire needling therapy. He proposed that the clinical application of fire needling is mainly based on pattern differentiation according to eight priciples, as well as in coordination with the theories of meridians and - organs. He believes that dry eye syndrome can be treated from the aspects of deficiency of liver and kidney, heart deficiency, and and deficiency. Through deeply digging the connotation of ancient classics, and combined his clinical practice using fire needling, professor created a unique therapy that select "Neichengqi"as the main acupoint combined with Xialeidian, Dagukong (EX-UE 5), Xiaogukong (EX-UE 6) and Shaoze (SI 1) in the treatment of dry eye syndrome. In this paper, from the etiology and pathogenesis of dry eye syndrome and therapeutic characteristics of fire needling, the thoughts of syndrome differentiation and characteristics of acupoints selection of professor were analyzed by a typical case, and it is expect to enrich the clinical treatment plan for dry eye syndrome.
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http://dx.doi.org/10.13703/j.0255-2930.2019.09.018DOI Listing
September 2019

Ultralow-Content Palladium Dispersed in Covalent Organic Framework for Highly Efficient and Selective Semihydrogenation of Alkynes.

Inorg Chem 2019 Aug 1;58(16):10829-10836. Epub 2019 Aug 1.

State Key Laboratory of Nuclear Resources and Environment, School of Chemistry, Biology and Materials Science , East China University of Technology , Nanchang 330013 , P. R. China.

Developing noble-metal-based catalysts with ultralow loading to achieve excellent performance for selective hydrogenation of alkynes under mild reaction conditions is highly desirable but still faces huge challenges. To this end, a SOH-anchored covalent organic framework (COF-SOH) as the support was deliberately designed, and then ultralow-content Pd (0.38 wt %) was loaded by a wet-chemistry immersion dispersion method. The resulting Pd/COF-SOH composite exhibits outstanding performance for the selective hydrogenation of phenylacetylene with 97.06% conversion and 93.15% selectivity to styrene under mild reaction conditions (1 bar of H, 25 °C). Noticeably, the turnover frequency value reaches as high as 3888 h, which outperforms most of reported catalysts for such use. Moreover, such a catalyst also exhibits excellent activity for a series of other alkynes and high stability without obvious loss of catalytic performance after five consecutive cycles.
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http://dx.doi.org/10.1021/acs.inorgchem.9b01117DOI Listing
August 2019

Long-term field insecticide susceptibility data and laboratory experiments reveal evidence for cross resistance to other neonicotinoids in the imidacloprid-resistant brown planthopper Nilaparvata lugens.

Pest Manag Sci 2020 Feb 2;76(2):480-486. Epub 2019 Aug 2.

Agro-Environment Research Division, Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization (NARO), Suya Kumamoto, Japan.

Background: Long-term monitoring data is helpful to understand the fluctuation of susceptibility and pattern of cross resistance in insecticide resistance management. After the occurrence of imidacloprid resistance, the brown planthopper (BPH) has gradually developed resistance to thiamethoxam and clothianidin since 2010, but not to dinotefuran and nitenpyram. Here, we analyzed susceptibilities data of five neonicotinoids during 2005-2017 in East Asia and Vietnam to conduct cross-resistance patterns among neonicotinoids. To determine the factors of development of cross resistance in laboratory bioassays, we used the imidacloprid resistant and control strains that were selected from filed populations in the Philippines and Vietnam.

Results: The Linear Mixed Models (LMM) analyses of insecticide susceptibility data showed that the slope values of imidacloprid resistance effects were 0.68 and 1.09 for resistance to thiamethoxam and clothianidin, respectively. Laboratory bioassay results showed that the LD values for thiamethoxam and clothianidin in resistant strains (1.4-5.5 μg g ) were 3.2-16.4 times higher than those in the control strains (0.28-1.5 μg g ). However, the increase in the LD values for imidacloprid was not related to that for dinotefuran and nitenpyram based on the results of the LMM analysis and laboratory bioassay.

Conclusion: Our results demonstrate that the development of imidacloprid resistance result in strong-cross resistance to some neonicotinoids, thiamethoxam and clothianidin, but not to others, dinotefuran and nitenpyram. We anticipate that our findings will be a starting point for understanding mechanism of the different trend of cross resistance by analyzing long-term susceptibility data and laboratory bioassays in insect pests. © 2019 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.5533DOI Listing
February 2020

[Spatial Variation and Environmental Significance of δO and δD Isotope Composition in Xijiang River].

Huan Jing Ke Xue 2017 Jun;38(6):2308-2316

Key Laboratory of Karst Dynamics, Ministry of Land and Resources & Guangxi, Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, China.

The H and O isotope composition of the Xijiang water was investigated on 54 samples collected from the mainstreams and main tributaries in June 2014 and January 2015.It was found that in the Xijiang river, there was a remarkable linear relationship between δ O and δD. This relationship was more significant in the dry season. In both seasons, the O and D values distributed along the meteoric water line, which indicated that precipitation was the source of the Xijiang River. Due to the direct water recharge through karst forms (i.e. sinkholes, vertical shaft and funnel) to the underground river, the -excess value was higher in the rainy season. While during the dry season, water recharge came from the storage in fissures and pores. Affected by altitude and evaporation effect, along the mainstream Hongshui River-Xunjiang and tributary Youjiang-Yujiang reach, the δ O and δD values were gradually approaching positive value in general. However, along the short tributary Guijiang reach, the altitude difference was not dramatic, and the discharge was less, the same phenomenon was not observed. By establishing a linear regression model between δ O and altitude, it revealed that the change rate of Xijiang water with altitude was -0.44‰·(100 m) in the rainy season, and -0.45‰·(100 m) in the dry season. The result reflected the height effect of the precipitation, which has a considerable meaning for the study of hydrological cycle in a river basin.
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http://dx.doi.org/10.13227/j.hjkx.201607136DOI Listing
June 2017

[Impacts of Thermal Stratification on the Hydrochemistry and Dissolved Inorganic Carbon in a Typical Karst Reservoir in Summer].

Huan Jing Ke Xue 2017 Aug;38(8):3209-3217

Key Laboratory of Karst Dynamics, Ministry of Land and Resources & Guangxi, Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, China.

Thermal stratification leads to significant stratification characteristics of hydrochemistry and aquatic organisms in reservoirs, and thus affects the biogeochemical cycle in the reservoir. This study aims to understand physico-chemical properties and dissolved inorganic carbon change processes and its factors in a karst groundwater-fed reservoir, Dalongdong Reservoir, located in Shanglin County, Guangxi Zhuang Autonomous Region, China. The eight sampling points were placed along the direction of the water flow on June 19-21, 2015. The results show that: ① The reservoir exhibited obvious thermal stratification in the summer. There were significant differences in physical and chemical parameters, such as pH and conductivity (Spc) between the epilimnion and thermocline; ② The dissolved oxygen (DO) and chlorophyll a (Chl-a) content from the surface to the bottom did not show a single decreasing trend, but the maximum value occurred 2.5 m or 5 m below the surface; ③ From the surface to the bottom, dissolved inorganic carbon (DIC) concentrations showed an increasing trend with the average DIC concentration of 2.03 mmol·L in the epilimnion and the average DIC concentration of 4.18 mmol·L at the bottom of the thermocline. The value of stable carbon isotope (δC) was more positive in the epilimnion than in the thermocline, where δC gradually became partially negative with water depth. Possible reasons of these results include: ① The significant differences in temperature, distribution of aquatic organisms, and strength and direction of metabolisms in different water layers due to thermal stratification; ② The DIC variations in the epilimnion were mainly affected by the carbonate precipitation process and phytoplankton photosynthesis, thereby affecting the DIC stable isotope fractionation. DIC was mainly controlled by biological respiration and the organic matter decomposition process in the thermocline.
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http://dx.doi.org/10.13227/j.hjkx.201703138DOI Listing
August 2017

[Spatial Variations of CO Degassing Across Water-air Interface and Its Impact Factors in Summer in Guijiang River, China].

Huan Jing Ke Xue 2017 Jul;38(7):2773-2783

School of Geographical Sciences, Southwest University, Chongqing 400715, China.

River is characterized by obvious spatial heterogeneity in catchment, which is exacerbated by special environment features of calcium-rich, alkaline and DIC-rich(dissolved inorganic carbon) in karst river. Thus, it also leads to significant spatial variation in the CO degassing across water-air interface. Main ions, physicochemical parameters, δC value and two common approaches(floating chamber(FC) and thin boundary layer models(TBL) were used to analyze the CO degassing characteristics in Guijiang River, a karst river, China. The results were as follows:1 Hydrochemistry in Guijiang River basin showed a significant spatial change. All of HCO, Ca, specific conductivity, total dissolved solids(TDS), SIc and CO showed similar distribution characteristics in the following order:tributaries in the middle reaches > middle reaches > Downstream > Upstream of Guijiang River. 2 During the monitoring period, CO degassing occurred in all the sampling sites and it was the CO source for the atmosphere. The mean CO evasion was 237 mg·(m·h) in Guijiang River, which located in the range of average CO evasion of global river. However, significant spatial variations also occurred along Guijiang River. The CO degassing flux in tributaries of the middle reaches and middle reaches of the mainstream were obviously larger than those in downstream and upstream of the mainstream. 3 CO degassing was mainly affected by carbonate equilibrium system in tributaries in the middle reaches and middle reaches in the mainstream of the Guijiang River basin, which resulted in obviously larger CO degassing than those in downstream and upstream of mainstream. However, the CO degassing flux in tributaries of the middle reaches was also simultaneously affected by biological photosynthesis, and the minimum CO degassing flux[6.38 mg·(m·h)] appeared in tributaries of the middle reaches. In addition, the CO degassing flux in mainstream upstream was mainly affected by atmospheric environmental factors, while it was synergetically influenced by many factors in mainstream downstream.
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http://dx.doi.org/10.13227/j.hjkx.201701114DOI Listing
July 2017

[Impacts of DES on the expressions of related genes in the gubernaculums testis of newborn mice].

Zhonghua Nan Ke Xue 2017 Jul;23(7):583-588

Department of Pediatric Surgery, Maternity and Child-Care Hospital, Shenzhen University, Shenzhen, Guangdong 518122, China.

Objective: To investigate the influence of diethylstilbestrol (DES) on the mRNA expressions of the androgen receptor (AR), estrogen receptor α (ERα), proliferating cell nuclear antigen (PCNA), and actin alpha 1 (ACTα1) in the gubernaculums testis of newborn mice and explore their action mechanisms.

Methods: A total of 140 male Kunming mice were randomly divided into a blank control, a dimethyl sulfoxide (DMSO) control, and 5 experimental groups to be treated subcutaneously with normal saline, DMSO, and DES at 0.02, 0.1, 0.5, 10 and 50 μg per kg of the body weight per day, respectively, at gestation days 9-17. On the first day after birth, the animals were sacrificed and the gubernaculums testis collected for detection of the mRNA expressions of AR, ERα, PCNA and ACTα1 by RT-PCR.

Results: Compared with the DMSO control, the experimental groups, particularly the DES 10 and 50 μg groups, showed significant increases in the mRNA expression of ERα (RE2 = 0.825, P <0.05), but remarkable decreases in those of AR, PCNA and ACTα1 (RA2 = 0.713, RP2 = 0.946, RT2 = 0.960, P <0.01), all in a dose-dependent manner.

Conclusions: The AR, ERα, PCNA, and ACTα1 mRNA are expressed in the gubernaculum testis of normal newborn mice, and their expression levels may be influenced by intervention with different concentrations of DES during the gestation. Exogenous estrogens may affect the proliferation and contraction of gubernaculum testis cells and consequently the normal development of the testis or even the whole male reproductive system by influencing the metabolism of ER and/or AR.
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July 2017

Elevated Levels of Soluble ST2 were Associated with Rheumatoid Arthritis Disease Activity and Ameliorated Inflammation in Synovial Fibroblasts.

Chin Med J (Engl) 2018 Feb;131(3):316-322

Department of Rheumatology and Immunology, The First People's Hospital of Yibin, Yibin, Sichuan 644000, China.

Background: Much evidence has demonstrated that interleukin (IL)-33 plays an important role in rheumatoid arthritis (RA). However, there have been limited studies about soluble ST2, a receptor for IL-33, in RA. The aims of this study were to detect the levels of ST2 in the serum and synovial fluid of RA patients and to reveal the association of these levels with disease activity and the function of ST2 in RA.

Methods: A total of 56 RA patients and 38 age-matched healthy controls were enrolled in this study. Synovial fluid samples were collected from another 30 RA patients and 20 osteoarthritis patients. Serum and synovial fluid levels of ST2 were measured by ELISA. In addition, the levels of ST2 in the serum of RA patients before and after therapy were detected. The function of ST2 in RA was revealed by the results of an in vitro cell assay, where recombinant ST2 proteins were used to treat peripheral blood mononuclear cells (PBMCs) and RA synovial fibroblasts (RASFs).

Results: Serum-soluble ST2 levels were significantly higher in RA patients (127.14 ± 61.43 pg/ml) than those in healthy controls (78.37 ± 41.93 pg/ml, P < 0.01). Synovial fluid-soluble ST2 levels (41.90 ± 33.58 pg/ml) were much higher in RA patients than those in osteoarthritis patients (19.71 ± 16.72 pg/ml, P < 0.05). RA patients who received effective therapy for 6 months showed decreased serum-soluble ST2 levels (113.01 ± 53.90 pg/ml) compared to baseline (139.59 ± 68.36 pg/ml) (P = 0.01). RA patients with high disease activity had higher serum-soluble ST2 levels (162.02 ± 56.78 pg/ml) than those with low disease activity (94.67 ± 40.27 pg/ml, P = 0.001). Soluble ST2 did not affect IL-1β, IL-6, IL-8, or tumor necrosis factor-α (TNF-α) expression in PBMCs from RA patients. However, soluble ST2 ameliorated the expressions of IL-33 and IL-1β but not that of IL-6, IL-8, or TNF-α in resting RASFs. Interestingly, in the RASFs stimulated by TNF-α plus IL-1β, soluble ST2 showed extensive suppressive effects on the expression of IL-6, IL-8, and TNF-α.

Conclusion: Elevated levels of ST2 in the serum and synovial fluid were associated with disease activity and ameliorated IL-33 expression and IL-33-induced inflammation in RASFs, suggesting that soluble ST2 might be a potential therapeutic candidate for RA.
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http://dx.doi.org/10.4103/0366-6999.223847DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5798053PMC
February 2018

The influence of microbial communities for triadimefon enantiomerization in soils with different pH values.

Chirality 2018 Mar 3;30(3):293-301. Epub 2018 Jan 3.

Department of Plant Protection, College of Plant Sciences and Technology, Huazhong Agricultural University, Wuhan, China.

Enantiomers of chiral molecules can undergo interconversion leading to markedly different toxicities, which can introduce significant uncertainty when evaluating biological and environmental fates. However, enantiomerization (the reversible conversion of one enantiomer into the other) related to soil microorganism is rarely understood. For better understanding, S-triadimefon and R-triadimefon enantiopure were incubated in different soils with different pH value. Both high-performance liquid chromatography and high-throughput sequencing technology were used to explore target analytes quantitatively and microbial taxa related to the conversion process. Results revealed a significant enantiomerization among the soils. The alkaline soil from Beijing had a faster conversion than neutral soil from Changchun, while acidic soil from Wuhan had no conversion. At the same results, analysis of bacteria community showed higher abundance of Arthrobacter and Halomonas genus in alkaline soil than neutral soil after treatments, but the acidic soil was lower. Moreover, Arthrobacter and Halomonas were responsible for converting S-triadimefon to R-triadimefon and R-triadimefon to S-triadimefon in alkaline and neutral soil, respectively. Thus, these genera may be one of the reasons to explain the enantiomerization in different soils observed in this study. Thus, research at microbial level is necessary for efficient ecological risk assessment of chiral fungicide.
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http://dx.doi.org/10.1002/chir.22796DOI Listing
March 2018

Diethylstilbestrol regulates mouse gubernaculum testis cell proliferation via PLC-Ca -CREB pathway.

Cell Biochem Funct 2018 Jan 26;36(1):13-17. Epub 2017 Dec 26.

Department of Pediatric Surgery, The Affiliated Maternal and Child Health Hospital of Shenzhen University Medical College, Shenzhen, China.

Recent evidence suggested a positive correlation between environmental estrogens (EEs) and high incidence of abnormalities in male urogenital system, but the mechanism remains unclear. Diethylstilbestrol (DES) is a nonsteroidal synthetic estrogen that disrupts the morphology and proliferation of gubernaculum testis cells, but the underlying mechanism is unclear. In this study, mouse gubernaculum testis cells were pretreated with phospholipase C (PLC) inhibitor U-73122 and then treated with DES. The results demonstrated that U-73122 impaired DES-evoked intracellular Ca2+ mobilization in gubernaculum testis cells and inhibited DES-induced proliferation of gubernaculum testis cells. Mechanistically, we found that U-73122 inhibited DES-induced activation of cAMP-response element binding protein (CREB) in gubernaculum testis cells. In conclusion, these data suggest that the effects of DES on mouse gubernaculum testis cells are mediated by PLC-Ca -CREB pathway.

Significance Of The Study: Environmental estrogens remain a serious threat to male reproductive health, and it is important to understand the mechanism by which EEs affect the male productive system. Here we explore potential mechanisms how the proliferation and contractility of gubernaculum testis cells are regulated by diethylstilbestrol. Our findings provide the first evidence that PLC-Ca -CREB signalling pathway mediates the nongenomic effects of diethylstilbestrol on gubernaculum testis cells. These findings provide new insight into the role of diethylstilbestrol in the aetiology of male reproductive dysfunction and will help develop better approaches for the prevention and therapy of male reproductive malformation.
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http://dx.doi.org/10.1002/cbf.3312DOI Listing
January 2018

Characterization of a Putative S-layer Protein of a Colonial Microcystis Strain.

Curr Microbiol 2018 Feb 27;75(2):173-178. Epub 2017 Sep 27.

Jiangsu Key Laboratory for Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210023, China.

Cell surface structure plays a key role in Microcystis colony formation. The S-layer is a crystalline array of monomolecular proteins that constitute the outermost component of the cyanobacterial cell envelope. To date, no biochemical characterization of the S-layer protein in Microcystis has been reported. Here, we compared S-layer on the cell wall of the unicellular strain Microcystis aeruginosa PCC7806 with the colonial strain M. aeruginosa XW01. We observed crystalline S-layers in XW01 cell walls; however, similar structures were not observed in PCC7806. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis revealed a thick putative S-layer protein band with an apparent molecular weight of 70 kDa extracted from XW01 cells, as well as an S-layer peptide fragment with the sequence ETYPLLAAPGAATDATR, similar to the translated product from PCC7806 unknown gene CAO89090.1. The amino acid composition of the translated CAO89090.1 product shared biochemical characteristics with those of bacterial S-layer proteins. Furthermore, a 1002-bp DNA fragment amplified from XW01 displayed 95% similarity with the CAO89090.1 gene, while the S-layer gene expression in XW01 was 36-fold higher than that observed in PCC7806. These data suggested that the S-layer protein plays a key role in Microcystis colony formation due to its significant contribution to cell surface hydrophobicity.
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http://dx.doi.org/10.1007/s00284-017-1362-3DOI Listing
February 2018

Insecticide susceptibilities of the two rice planthoppers Nilaparvata lugens and Sogatella furcifera in East Asia, the Red River Delta, and the Mekong Delta.

Pest Manag Sci 2018 Feb 2;74(2):456-464. Epub 2017 Nov 2.

Chiayi Agricultural Experiment Station, Taiwan Agricultural Research Institute, Council of Agriculture, Chiayi, Taiwan, China.

Background: The two rice planthoppers, Nilaparvata lugens and Sogatella furcifera, have different life cycles in the regions of East Asia, the Red River Delta, and the Mekong Delta. The susceptibilities of these species to a range of insecticides have not previously been compared among the three regions over multiple years. Here, we describe the differences and similarities in insecticide susceptibilities of the two species among the three regions in 2006-2011.

Results: In all three regions in 2006 - 2011, N. lugens developed high and moderate levels of resistance to imidacloprid and thiamethoxam, respectively, but this species did not develop resistance to fipronil. In contrast, S. furcifera developed a high level of resistance to fipronil. The ranges in 50% lethal dose (LD ) values for N. lugens treated with both imidacloprid and thiamethoxam were similar over time between East Asia and the Red River Delta, and were different in the Mekong Delta.

Conclusion: The results support the idea that resistant populations migrate from the Red River Delta region to East Asia. Therefore, continuous monitoring of the susceptibility of N. lugens to insecticides in the Red River Delta is very important for insecticide resistance management in East Asia. © 2017 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.4729DOI Listing
February 2018

Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of Heliothis virescens ascovirus 3 h (HvAV-3h).

Sci Rep 2017 08 1;7(1):7045. Epub 2017 Aug 1.

Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.

Ascoviruses are double-stranded DNA viruses that mainly infect noctuid larvae, and are transmitted by the parasitoid wasp Microplitis similis Lyle. Ascovirus-parasitoids wasp-noctuid insects constitute the dissemination system. Selection of suitable reference genes for the dissemination system could play an important role in elucidating the pathogenic molecular mechanisms of ascovirus. Unfortunately, such studies on potential reference genes in the dissemination system of ascoviruses are lacking. In the present study, we evaluated 11 candidate reference genes: β-actin1 (ACT1), β-actin2 (ACT2), elongation factor 1 (EF1), elongation factor 2 (EF2), ribosomal protein L10 (L10), ribosomal protein L17A (L17A), superoxide dismutase (SOD), 28S ribosome (28S), Tubulin (TUB) and 18S ribosome (18S). The samples were originally from various virus concentrations and points-in-time of experimental treatments using RefFinder and four algorithms. The results showed that EF1 was the most stable internal gene in S. exigua and M. similis and that EF2 was the most stable in the IOZCAS-Spex-II-A cell line, and the stability of reference genes were confirmed via the expression levels of two inhibitor of apoptosis-like (iap-like) genes from Heliothis virescens ascovirus 3 h (HvAV-3h). This study provides a crucial basis for future research that explores the molecular mechanisms of the pathogenesis of ascoviruses.
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http://dx.doi.org/10.1038/s41598-017-07684-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539149PMC
August 2017

Effects of confinement duration and parity on stereotypic behavioral and physiological responses of pregnant sows.

Physiol Behav 2017 Oct 11;179:369-376. Epub 2017 Jul 11.

College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China. Electronic address:

The aim of this study was to evaluate the effects of space restriction stress on the stereotypic behavioral and physiological responses of different parity sows, and it is necessary to understand such effects due to space restriction to improve the welfare of the sows in confined conditions. We selected fifty pregnant sows (Large White) at primiparity and first to fifth parity in a confined farm with the same body condition and due date (3±1.5days). Behavioral observations and physiological analysis were carried out during spatial confinement throughout pregnancy. The results showed that there were no significant changes in vacuum-chewing, bar-biting, trough-biting and the concentrations of serum IL-6 in primiparous sows during the initial confinement (0-8days). With the increase of the confinement duration, the serum cortisol, IgA, IL-6 concentrations and the vacuum-chewing frequency of sows in all groups increased significantly, and the serum concentrations of C-reactive protein and Pig-MAP increased significantly except for the sows in the first and second parity groups. The serum cortisol, IgA, IL-6 concentrations and the vacuum-chewing frequency of older sows were significantly higher than those of the young sows throughout the entire restricted feeding period, but the serum C-reactive protein concentrations of primiparous gilts was significantly higher than those of the other groups. The serum cortisol, IgA, IL-6 concentrations and bar-biting and trough-biting frequencies of all parity sows decreased significantly after entering the delivery bed. In conclusion, long-lasting and recurrent environmental constraints can significantly increase the frequency of stereotypical behavior and serious psychological and physical stress, seriously damaging sow welfare.
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http://dx.doi.org/10.1016/j.physbeh.2017.07.015DOI Listing
October 2017

Prediction of 10-year Atherosclerotic Cardiovascular Disease Risk among Adults Aged 40-79 Years in China: a Nationally Representative Survey.

Biomed Environ Sci 2017 Apr;30(4):244-254

Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: To establish the distribution of 10-year atherosclerotic cardiovascular disease (ASCVD) risk among Chinese adults.

Methods: We estimated the 10-year ASCVD risk by applying the 2013 American College of Cardiology/ American Heart Association pooled cohort equations (PCEs) to the data obtained from the 2010 China Chronic Disease and Risk Factor Surveillance that involved 61,541 participants (representing 520,158,652 Chinese adults) aged 40-79 years. We also compared the ASCVD risk with the 10-year ischemic cardiovascular disease (ICVD) risk, which was calculated using the simplified scoring tables recommended by the Chinese Guidelines for Prevention of Cardiovascular Diseases (Chinese model).

Results: Based on the PCEs, the average 10-year ASCVD risk among adults without self-reported stroke or myocardial infraction was 12.5%. Approximately 247 million (47.4%) and 107 million (20.6%) adults had ⋝ 7.5% and > 20% 10-year ASCVD risks, respectively. The 10-year ASCVD risk > 20% was higher among men, less educated individuals, smokers, drinkers, and physically inactive individuals than among their counterparts. Overall, 29.0% of adults categorized using the Chinese model were overclassified with the PCEs.

Conclusion: Our results define the distribution of 10-year ASCVD risk among Chinese adults. The 10-year ASCVD risk predicted by the PCEs was higher than the ICVD risk predicted by the Chinese model.
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http://dx.doi.org/10.3967/bes2017.034DOI Listing
April 2017

Peutz-Jeghers syndrome with intermittent upper intestinal obstruction: A case report and review of the literature.

Medicine (Baltimore) 2017 Apr;96(17):e6538

Department of Pediatric Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China.

Rationale: Peutz-Jeghers syndrome (PJS) is an autosomal dominant genetic syndrome characterized by a unique type of gastrointestinal hamartomatous polyp associated with oral and anal mucocutaneous pigmentations. Peutz-Jeghers polyps occur most numerously in the small intestine but frequently in the colon and stomach, only a few cases have been reported in the duodenum.

Patient Concern: A further family history survey discovered 10 out of 14 members of the family (in 4 generations) had mucocutaneous pigmentations, but many of them were living in rural areas where they had no access to specialized medical services, so none were checked with endoscopy for polyps of hamartoma.

Diagnoses: We report the case of a boy patient with mucocutaneous pigmentations over the lips, and a history of recurrent bouts of vomit and anemia over the preceding two years, no abdominal pain and mass. An upper gastrointestinal endoscopy revealed some small polyps in the stomach and multiple sessile polyps in the second part of the duodenum, but colonoscopy exam did not reveal any lesion.

Interventions: A double polypectomy and duodenum segmentary resection with end-to-end anastomosis was performed. Histopathology of the resected duodenum polyps indicated it to be a typical hamartomatous polyp.

Outcomes: The child was under regular follow-up and recovered well.

Lessons: In this case, the patient was characteristic with pigmentations on his lips and intermittent upper intestinal obstruction (due to mass duodenal polyps), there are no definitive guidelines for the treatment to duodenal PJS hamartomatous polyp, each case requires tailor-made management.
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http://dx.doi.org/10.1097/MD.0000000000006538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5413220PMC
April 2017

Gender-specific Association of Sleep Duration with Body Mass Index, Waist Circumference, and Body Fat in Chinese Adults.

Biomed Environ Sci 2017 Mar;30(3):157-169

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: To examine the association between habitual sleep duration and obesity among Chinese adults.

Methods: The association of sleep duration and obesity was investigated among 7,094 community-dwelling Chinese adults. Sleep duration was self-reported. In this study, obesity was defined as follows: body mass index (BMI) ⋝ 28 kg/m2, waist circumference (WC) ⋝ 85 cm in men and ⋝ 80 cm in women, and percent body fat (%BF) ⋝ 25 in men and ⋝ 35 in women. Logistic and quantile regressions were employed to examine relationships of interest.

Results: Overall, 6.42% of the participants reported short sleep durations (< 6 h/d) while 14.71% reported long (⋝ 9 h/d) sleep durations. Long sleepers (⋝ 9 h/d) represented a greater frequency of women with obesity [odds ratio (OR): 1.30; 95% confidence interval (CI), 1.02-1.67] and high body fat (1.43, 1.04-1.96) than those who slept 7-8 h/d. An association between long sleep times and higher BMI estimations was found across the 10th-75th percentile of the BMI distribution. Among men, long sleepers (⋝ 9 h/d) presented lower risks of developing abdominal obesity compared with individuals who slept 7-8 h/d (OR = 0.79, 95% CI: 0.44-0.99).

Conclusion: Our study suggests that long sleep durations are associated with general obesity in Chinese women but reduced waist circumferences in men. Confirmatory studies are needed to determine the heterogeneous association of sleep time and obesity by gender.
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http://dx.doi.org/10.3967/bes2017.023DOI Listing
March 2017

[Temporal and Spatial Variations of Dissolved Inorganic Carbon and Its Stable Isotopic Composition in the Surface Stream of Karst Groundwater Recharge].

Huan Jing Ke Xue 2017 Feb;38(2):527-534

Key Laboratory of Karst Dynamics, Ministry of Land and Resources & Guangxi, Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, China.

Stable carbon isotope of dissolved inorganic carbon (δC), which is mainly constituted by HCO in karst water, is widely used to trace the different sources and influential factors of dissolved inorganic carbon (DIC). In order to understand the distribution of DIC and δC in subtropical karst area, this paper researched the water chemistry and δC in a karst surface stream in detail, which is fed by Guancun subterranean stream in Liuzhou City, Guangxi Province, in the southwest of China. The results showed that the contents of DIC in subterranean stream outlet (G1 site) ranged from 4.60 to 4.90 mmol·L with an average of 4.73 mmol·L in dry season, and from 2.80 to 4.70 mmol·L with an average of 4.23 mmol·L in rainy season. The contents of DIC in 1.35 km downstream site (G2 site) ranged from 4.30 to 4.90 mmol·L with an average of 4.56 mmol·L in dry season, and from 3.00 to 4.70 mmol·L with an average of 4.20 mmol·L in rainy season. The δC of subterranean stream outlet (G1 site) varied from -12.8‰ to -11.53‰ with an average of -12.22‰ in dry season, and from -13.12‰ to -11.01‰ with an average of -12.28‰ in rainy season. The δC of stream downstream site (G2 site) ranged from -11.71‰ to -9.55‰ with an average of -10.73‰ in dry season, and ranged from -12.18‰ to -9.85‰ with an average of -11.10‰ in rainy season. The contents of DIC of G1 site were higher than those of G2 site. The DIC contents in dry season in both G1 and G2 site were higher than those in rainy season. The values of δCof G1 and G2 site in dry season were more positive than those in rainy season. The δCvalue of G1 site was more negative than that of G2 site. The main sources of DIC in underground river and surface stream were soil COand carbonate dissolution. However, the differences of DIC and δCbetween G1 and G2 site showed that COdegassing and photosynthesis of aquatic plants had significant influence on water DIC and δC value. This study is helpful to understand the dynamic change and distribution of DIC and δC in karst surface stream.
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http://dx.doi.org/10.13227/j.hjkx.201607171DOI Listing
February 2017

Diagnosis and treatment of pediatric benign pneumoperitoneum: A case report series of 9 patients.

Medicine (Baltimore) 2017 Jan;96(2):e5814

aDepartment of Pediatric Surgery, The Second Affiliated Hospital of Shantou University Medical College bDepartment of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China.

Introduction: Benign pneumoperitoneum (BPPT) is defined as asymptomatic free intraabdominal air or as pneumoperitoneum without peritonitis. Symptomatic free air requires surgical anagement, but management of asymptomatic pneumoperitoneum is controversial. In this study, we investigate the diagnosis and treatment of BPPT in children.

Clinical Findings: The clinical data of 9 pediatric patients with BPPT who were admitted to our hospital from January 2000 to January 2015 were retrospectively analyzed to summarize the diagnosis and treatment. Overall, 9 cases were included with 8 males and 1 female, aged from 4 days to 4 years. Among them there were 6 newborns (including 1 premature infant). Patients were all admitted to hospital with the major clinical symptom of abdominal distension, including 2 cases accompanied by tachypnea, 2 cases with vomiting, 1 case with diarrhea, and 2 cases with fever. No previous constipation or obstructive defecation existed. Six newborns had meconium defecation within 24 hours after birth. Physical examination revealed all patients with relaxed abdominal wall except 1 patient with abdominal distension had slight muscle stiffness and hyperactive bowel sounds. Abdominal X-ray suggested free air under the diaphragm in all cases.

Interventions/outcomes: All patients except for one case of laparotomy were conservatively treated and cured with fasting, infection prevention, rehydration, abdominocentesis, and close observation. Nine cases of patients were all discharged with no death occurrence. After discharge follow-up of 7 months to 6 years was conducted. There was no recurrence of similar symptoms, and children were in good growth and development.

Conclusion: The diagnosis of BPPT mainly relies on clinical symptoms in patient, careful abdominal examination, abdominal X-ray combined with abdominocentesis, and the exclusion of gastrointestinal perforation for confirmation. Conservative treatment can cure the disease. Attention should be paid to distinguish with surgical pneumoperitoneum to avoid unnecessary surgical exploration.
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http://dx.doi.org/10.1097/MD.0000000000005814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5266170PMC
January 2017

[Sources and Variation Characteristics of Dissolved Lipid Biomarkers in a Typical Karst Underground River].

Huan Jing Ke Xue 2016 May;37(5):1814-22

Water samples in Qingmuguan underground river were collected to determine the concentration of dissolved lipids, and their sources, composition and migration characteristics in underground river were studied. The results were obtained as follows. (1) The average content of various dissolved lipids decreased with increasing distance of migration in Qingmuguan underground river, and the most distinctive was dissolved saturated straight chain fatty acids with its content decreasing by about 81.71%, from the initial 5,704 ng · L⁻¹ to 1,043 ng · L⁻¹. (2) N-alkane could indicate the sources of dissolved organic matter in underground river, but saturated straight chain fatty acid and fatty alcohol had the advantage in indicating algae, bacteria and other microorganisms. (3) With the increasing migration distance of Qingmuguan underground river, the input type of dissolved organic matter in underground river was different, which might be resulted from the heterogeneity of karst surface. (4) In ternary plot of alkane sources, aquatic plants constituted the major contribution of dissolved organic matter in Qingmuguan underground river, followed by higher plant, algae/ bacteria. Unlike higher plants and algae/bacteria, the contribution from aquatic plants decreased with increasing migration distance of underground river. (5) To a certain extent, TAR (alkane) values could reflect the rainfall, and the values of CPI, L/H in saturated straight chain fatty acids might indicate the degradation activities of bacteria.
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May 2016

[Variations of CO Exchange Fluxes Across Water-air Interface and Environmental Meaning in a Surface Stream in Subtropical Karst Area, SW China].

Huan Jing Ke Xue 2016 Jul;37(7):2487-2495

School of Geographical Sciences, Southwest University, Chongqing 400715, China.

CO cycle process or sources/sinks are not only the basis of understanding and responding to global climate change, but also the core of the current global climate change research. Gas exchange across water-air interface in terrestrial surface water is an important way of nutrient elements (carbon, nitrogen) exchange between aquatic ecosystems and ambient air. Escaping CO gas from surface water is also actively involved in the modern carbon cycle. In the material cycle in karst regions, CO plays a key role in karst processes, driving the formation of karst features. Karst groundwater with high water CO partial pressure (CO) often shows highly positive CO concentration gradient to atmosphere after it is discharged to surface, so the evaluation of CO exchange fluxes across karst water-air interface is important for karst carbon cycle research. This paper researched CO exchange fluxes across water-air interface in the karst surface stream in detail which was fed by Guancun subterranean stream in Liuzhou city, Guangxi province. Closed static chamber method and portable hand-holding CO sensor (GM70) were both employed in CO exchange fluxes monitoring. The results showed that CO degassing was the mainly form of CO exchange across the steam water-air interface. CO degassing flux in subterranean stream outlet (G1 site) ranged from 139.48 to 890.84 mg·(m·h) with an average of 445.72 mg·(m·h). CO degassing flux in stream downstream site (G2 site) ranged from 16.54 to 844.18 mg·(m·h) with an average of 159.81 mg·(m·h). The CO degassing flux in G1site was higher than that in G2 site. CO degassing fluxes in rainy season in both G1 and G2 site were higher than those in dry season. Stable carbon analysis of CO gas (δC-CO) found that CO degassing from karst stream might influence air CO carbon isotope near water surface, which resulted in the more negative δC-CO value with the increase of CO degassing flux. Significant spatio-temporal variations of δC-CO were found, and the δC-CO in the rainy season was more negative than that in dry season and δC-CO in G1 site was more negative than that in G2 site. As a result of stream CO degassing, the hydrochemical characteristics of steam varied along stream running, which resulted in decrease of HCO, EC and CO and increase of pH, SIc and δC-DIC in the stream.
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http://dx.doi.org/10.13227/j.hjkx.2016.07.010DOI Listing
July 2016

Prevalence of Major Cardiovascular Risk Factors and Cardiovascular Disease in Women in China: Surveillance Efforts.

Biomed Environ Sci 2016 Mar;29(3):205-11

National Center for Chronic and Noncommunicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

In this study, we aimed to assess the relationship of socioeconomic status and acculturation with cardiovascular disease (CVD) risk profiles and CVD and examine the CVD risk factors associated with CVD. We used data from the 2010 China Chronic Disease and Risk Factor Surveillance surveys, which consisted of a nationally representative sample of women. The following prevalence was found: myocardial infarction (MI): 0.4%; stroke: 0.5%; abnormal cholesterolemia: 44.9%; overweight or obesity: 32.2%; hypertension: 31.7%; diabetes: 9.0%; and smoking: 2.5%. In total, 30.9% of Chinese women had no risk factors, but 13.3% had ⋝3 associated risk factors. In multivariate-adjusted models, hypertension, diabetes, overweight or obese, and smoking were all directly associated with MI; For stroke, associations were positive with hypertension, abnormal cholesterolemia, diabetes, and overweight or obesity. Therefore, it can be concluded that CVD risk factors are common among Chinese women aged ⋝18 years.
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http://dx.doi.org/10.3967/bes2015.025DOI Listing
March 2016

Growth and antioxidant production of Spirulina in different NaCl concentrations.

Biotechnol Lett 2016 Jul 30;38(7):1089-96. Epub 2016 Mar 30.

Jiangsu Key Laboratory for Biodiversity and Biotechnology, School of Life Sciences, Nanjing Normal University, Nanjing, 210023, China.

Objective: To evaluate the quantity of Spirulina cultured in seawater, salt-tolerant strains were screened out and their growth and antioxidant accumulation were studied in different salt concentrations

Results: Salt tolerance of five Spirulina strains were investigated with modified Zarrouk medium (with 200-800 mM NaCl). All strains grew well with 400 mM NaCl; their growth rates were almost same as in the control medium. Spirulina strains FACHB-843 (SP843) and FACHB-972 (SP972) had the highest salt tolerance their growth rates in 600 mM NaCl were nearly same as the control. Both strains produced more carotene, phycocyanin, polysaccharides, proline and betaine in 400-600 mM NaCl than the control. Salt stress also induced them to produce higher activities of superoxide dismutase and peroxidase. Total antioxidant capacities of SP843 and SP972 peaked at 600 and 400 mM NaCl, respectively.

Conclusion: Spirulina strains cultured with seawater accumulate more bioactive substances and will have a higher nutritive value.
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http://dx.doi.org/10.1007/s10529-016-2087-2DOI Listing
July 2016

[Summer Greenhouse Gases Exchange Flux Across Water-air Interface in Three Water Reservoirs Located in Different Geologic Setting in Guangxi, China].

Huan Jing Ke Xue 2015 Nov;36(11):4032-42

Due to special hydrogeochemical characteristics of calcium-rich, alkaline and DIC-rich ( dissolved inorganic carbon) environment controlled by the weathering products from carbonate rock, the exchange characteristics, processes and controlling factors of greenhouse gas (CO2 and CH4) across water-air interface in karst water reservoir show obvious differences from those of non-karst water reservoir. Three water reservoirs (Dalongdong reservoir-karst reservoir, Wulixia reservoir--semi karst reservoir, Si'anjiang reservoir-non-karst reservoir) located in different geologic setting in Guangxi Zhuang Autonomous Region, China were chosen to reveal characteristics and controlling factors of greenhouse gas exchange flux across water-air interface. Two common approaches, floating chamber (FC) and thin boundary layer models (TBL), were employed to research and contrast greenhouse gas exchange flux across water-air interface from three reservoirs. The results showed that: (1) surface-layer water in reservoir area and discharging water under dam in Dalongdong water reservoir were the source of atmospheric CO2 and CH4. Surface-layer water in reservoir area in Wulixia water reservoir was the sink of atmospheric CO2 and the source of atmospheric CH4, while discharging water under dam was the source of atmospheric CO2 and CH4. Surface-layer water in Si'anjiang water reservoir was the sink of atmospheric CO2 and source of atmospheric CH4. (2) CO2 and CH4 effluxes in discharging water under dam were much more than those in surface-layer water in reservoir area regardless of karst reservoir or non karst reservoir. Accordingly, more attention should be paid to the CO2 and CH4 emission from discharging water under dam. (3) In the absence of submerged soil organic matters and plants, the difference of CH4 effluxes between karst groundwater-fed reservoir ( Dalongdong water reservoir) and non-karst area ( Wulixia water reservoir and Si'anjiang water reservoir) was less. However, CO2 efflux in karst groundwater-fed reservoir was much higher than that of reservoir in non-karst area due to groundwater of DIC-rich input from karst aquifer and thermal stratification.
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November 2015

Synthesis and Herbicidal Activity of 5-Heterocycloxy-3-methyl-1-substituted-1H-pyrazoles.

Molecules 2015 Dec 25;21(1):E39. Epub 2015 Dec 25.

Department of Plant Protection, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

With the objective of finding valuable herbicidal candidates, a series of new 5-heterocycloxy-3-methyl-1-substituted-1H-pyrazoles were synthesized and their herbicidal activities were evaluated. The bioassay results showed that some compounds exhibited excellent herbicidal activities at the concentration of 100 mg/L, and compound 5-chloro-2-((3-methyl-1-(2,2,2-trifluoroethyl)-1H-pyrazol-5-yl)oxy)pyrimidine showed bleaching activity to green weeds. In greenhouse conditions, this compound also showed excellent post-emergence herbicidal effect against Digitaria sanguinalis L. at the dosage of 750 g a. i. ha(-1).
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http://dx.doi.org/10.3390/molecules21010039DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273275PMC
December 2015

[Variations of Inorganic Carbon and its Impact Factors in Surface-Layer Waters in a Groundwater-Fed Reservoir in Karst Area, SW China].

Huan Jing Ke Xue 2015 Aug;36(8):2833-42

In order to understand the inorganic carbon cycle of the groundwater-fed reservoir in karst area, Dalongdong Reservoir, which is located at Shanglin County, Guangxi Zhuang Autonomous Region, China, was investigated from 12th to 20th July, 2014. Concentration of dissolved inorganic carbon (DIC), delta13C of DIC (delta13C(DIC)), partial CO2 pressure (pCO2) and CO2 flux across water-air interface were studied by observation in situ and high-resolution diel monitoring. Results show that: (1) DIC concentration and water pCO2 increased from upstream area to downstream area [DIC(average)): from 122.88 to 172.02 mg x L(-1), pCO2(average) : from 637.91 x 10(-6) to 1399.97 x 10(-6)], while delta13C(DIC) decreased from upstream area to downstream area [delta13C(DIC(average): from -4.34% per hundred to -6.97% per hundred] in the reservoir. (2) CO2 efflux across water-air interface varied from 7.11 to 335.54 mg x (m2 x h)(-1) with mean of 125.03 mg x (m2 x h)(-1) in Dalongdong reservoir surface-layer waters, which was the source of atmospheric CO2. CO2 effluxes across water-air interface in upstream area [mean 131.73 mg x (m2 x h)(-1)] and downstream area [mean 170.25 mg x (m2 x h)(-1)] were higher than that in middle area [mean 116.05 mg x (m2 x h))(-1)] in the reservoir. (3) Water pCO2 and CO2 efflux across water-air interface showed similar characteristics of diel variations, which decreased in daylight and increased in night and showed a negative correlation with chlorophyll a (Chla). Possible reasons of research results are found as follows: (1) DIC concentration, water pCO2 and delta13C(DIC) are influenced by biomass of phytoplankton, turbidity, conductivity, the depth of water and transparency, while CO2 efflux across water-air interface is controlled by both of biomass of phytoplankton and wind speed. (2) Photosynthesis, respiration and vertical motion of phytoplankton possibly affect diel variations of DIC cycle in the groundwater-fed reservoir in karst area.
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August 2015