Publications by authors named "Jian-Feng Sun"

14 Publications

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[Analysis of chemical constituents of Ginseng-Douchi compound fermentation products based on UPLC-Q-TOF-MS].

Zhongguo Zhong Yao Za Zhi 2021 Mar;46(6):1417-1429

Institute of Chinese Materia Medica,Heilongjiang Academy of Chinese Medical Sciences Harbin 150036,China.

In this experiment, ultra high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used to analyze and identify chemical constituents of Ginseng-Douchi(GD) compound fermentation, and explore the conversion rules of ginsenosides and soybean isoflavones after compound fermentation. Waters Acquity UPLC BEH C_(18) column(2.1 mm×100 mm, 1.7 μm) was adopted, with 0.1% formic acid aqueous solution(A)-0.1% formic acid acetonitrile solution(B) as mobile phase for gradient elution; electrospray ion source(ESI) was used to collect data in positive and negative ion modes; according to the exact mass number, the secondary spectrum comparison of the database and the existing literature reports, Peakview 2.0/masterview 1.0 software was used to determine the common ion structure formula. Finally, a total of 133 chemical constituents were analyzed and identified from the GD. Ginseng saponins and isoflavone glycosides were significantly converted after fermentation. Among them, peak areas of prototype ginsenosides Rk_3, Rh_1, Rh_2, Rh_3, daidzin, glycitin and genistin decreased significantly; whereas peak areas of se-condary ginsenoside Rb_1, Rb_2, Rk_1, glycitein, genistein and daidzein increased significantly. In this experiment, liquid-mass spectrometry technique was used to investigate the conversion of active ingredients of GD compound fermented products after co-fermentation, so as to provide a scientific basis for elucidating pharmacodynamics material basis and quality control.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210223.301DOI Listing
March 2021

Two-one-way laser Doppler approach for inter-satellite velocity measurement.

Opt Express 2019 Jan;27(2):1353-1366

A high-precision inter-satellite velocity measurement method based on two-one-way laser Doppler is presented in this paper. This method's working principle and signal-to-noise ratio's effect under different measurement times of signal on velocity precision are analyzed theoretically. This method is also tested by laboratory experiments and 1 mm/s velocity precision is achieved in 1 ms integrating time. The proposed method potentially contributes to inter-satellite velocity measurement, especially for the relative velocity measurement between two satellites in high dynamic motion and a long distance apart.
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http://dx.doi.org/10.1364/OE.27.001353DOI Listing
January 2019

Review of Anemone raddeana Rhizome and its pharmacological effects.

Chin J Integr Med 2018 Jan 6;24(1):72-79. Epub 2017 Oct 6.

Medical College, Hangzhou Normal University, Hangzhou, 311121, China.

The chemical compositions of Anemone raddeana Rhizome, a kind of traditional Chinese medicine, were reviewed, along with its bioactivity and pharmacological properties and method improvements of extracting and detecting triterpenoid saponins. A. raddeana Rhizome is used to treat neuralgia and rheumatism, and is rich in triterpenoid saponins, most of which are pentacyclic, with oleanane as the nucleus. So far, 37 triterpenoid saponins have been determined from the herb. Its reported bioactivity and pharmacological properties have been described as anticancerous, antimicrobial, anti-inflammatory, analgesic, antipyretic, anticonvulsive, antihistaminic, and sedative. It has also been used for the induction of the humoral immune response and treatment of liver fibrosis in chronic hepatitis. However, the herb also has hemolytic effects and can be toxic, which limits its clinical application. Further studies are needed on the pharmaceutical functions, mechanisms, and immunological responses to contribute to the herb's clinical applications.
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http://dx.doi.org/10.1007/s11655-017-2901-2DOI Listing
January 2018

Hydrogen-rich saline injection into the subarachnoid cavity within 2 weeks promotes recovery after acute spinal cord injury.

Neural Regen Res 2015 Jun;10(6):958-64

Department of Orthopedics, the Third Xiangya Hospital of Central South University, Changsha, Hunan Province, China.

Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that hydrogen-rich saline would promote the repair of spinal cord injury induced by Allen's method in rats. At 0.5, 1, 2, 4, 8, 12 and 24 hours after injury, then once daily for 2 weeks, 0.25 mL/kg hydrogen-rich saline was infused into the subarachnoid space through a catheter. Results at 24 hours, 48 hours, 1 week and 2 weeks after injury showed that hydrogen-rich saline markedly reduced cell death, inflammatory cell infiltration, serum malondialdehyde content, and caspase-3 immunoreactivity, elevated serum superoxide dismutase activity and calcitonin gene-related peptide immunoreactivity, and improved motor function in the hindlimb. The present study confirms that hydrogen-rich saline injected within 2 weeks of injury effectively contributes to the repair of spinal cord injury in the acute stage.
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http://dx.doi.org/10.4103/1673-5374.158361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498359PMC
June 2015

Model-based recognition of 3D articulated target using ladar range data.

Appl Opt 2015 Jun;54(17):5382-91

Ladar is suitable for 3D target recognition because ladar range images can provide rich 3D geometric surface information of targets. In this paper, we propose a part-based 3D model matching technique to recognize articulated ground military vehicles in ladar range images. The key of this approach is to solve the decomposition and pose estimation of articulated parts of targets. The articulated components were decomposed into isolate parts based on 3D geometric properties of targets, such as surface point normals, data histogram distribution, and data distance relationships. The corresponding poses of these separate parts were estimated through the linear characteristics of barrels. According to these pose parameters, all parts of the target were roughly aligned to 3D point cloud models in a library and fine matching was finally performed to accomplish 3D articulated target recognition. The recognition performance was evaluated with 1728 ladar range images of eight different articulated military vehicles with various part types and orientations. Experimental results demonstrated that the proposed approach achieved a high recognition rate.
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http://dx.doi.org/10.1364/AO.54.005382DOI Listing
June 2015

Pharmacological characterization and therapeutic potential for the treatment of opioid abuse with ATPM-ET, an N-ethyl substituted aminothiazolomorphinan with κ agonist and μ agonist/antagonist activity.

Eur J Pharmacol 2014 Oct 3;740:455-63. Epub 2014 Jul 3.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China. Electronic address:

We previously reported that the κ agonists with mixed μ activity could attenuate heroin self-administration with less potential to develop tolerance. The present study further investigated the effects of (-)-3-N-Ethylamino-thiazolo[5,4-b]-N-cyclopropylmethylmorphinan hydrochloride (ATPM-ET), a κ agonist and μ agonist/antagonist, on the acquisition and reinstatement of morphine-induced conditioned place preference (CPP), heroin self-administration and heroin-primed reinstatement of drug-seeking behavior. We found that ATPM-ET produced a longer duration of potent antinociceptive effects with less side effect of sedation. More importantly, ATPM-ET attenuated the acquisition of morphine-induced CPP, without affecting the reinstatement of morphine CPP. Furthermore, ATPM-ET significantly inhibited heroin self-administration and the reinstatement of heroin primed drug-seeking behavior. Taken together, ATPM-ET, a novel κ agonist and μ agonist/antagonist may have utility for the treatment of drug dependence.
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http://dx.doi.org/10.1016/j.ejphar.2014.06.045DOI Listing
October 2014

[Spatial distribution of sulfur dioxide around a tobacco bulk-curing workshop cluster].

Ying Yong Sheng Tai Xue Bao 2014 Mar;25(3):857-62

In order to manifest lower energy consumption and less labor employment, and provide the theoretical basis for constructing environmentally friendly modem tobacco agriculture, this paper analyzed gas composition of the chimney from a bulk-curing barn and the dispersion of sulfur dioxide (SO2) around the workshop cluster using ecom-J2KN flue gas analyzer and air sampler. During curing, the concentrations of carbon dioxide (CO2) and SO2 in the chimney were both highest at 38 degrees C, while the concentration of nitrogen oxides (NOx) was highest at 42 degrees C. The emission concentration of SO2 from the chimney was 1327.60-2218.40 mg x m(-3). Average SO2 emission would decrease by 49.7% through adding 4.0% of a sulfur-fixed agent. The highest concentrations of SO2 in the surface soil appeared at the yellowing stage. SO2 concentration in horizontal direction localized at 43-80 m exceeded 0.5 mg x m(-3). The highest concentration of SO2 (0.57 mg x m(-3)) was observed at 50 m. At 50 m in the downstream wind direction of the workshop cluster, SO2 concentration in vertical direction localized at 0.9-1.8 m exceeded 0.5 mg x m(-3), and the highest concentration of SO2 in vertical direction was 0.65 mg x m(-3) at 1.6 m. During curing, the average concentration of SO2 was decreased by 0.43 mg x m(-3) by using the sulfur-fixed agent. The polluted boundary was localized at 120 m in the downstream wind direction of the workshop cluster.
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March 2014

3D pose estimation of ground rigid target based on ladar range image.

Appl Opt 2013 Nov;52(33):8073-81

In the target recognition of laser radar (ladar), the accurate estimation of target pose can effectively simplify the recognition process. To achieve 3D pose estimation of rigid objects on the ground and simplify the complexity of the algorithm, a novel pose estimation method is proposed in this paper. In this approach, based on the feature that most rigid objects on the ground have large planar areas which are horizontal on the top of the targets and vertical sides and combined with the 3D geometric characteristics of ladar range images, the planar normals of rigid targets were adopted as the vectors in the positive direction of the axes in the model coordinate system to estimate the 3D pose angles of targets. The simulation experiments were performed with six military vehicle models and the performance in self-occlusion, occlusion, and noise was investigated. The results show that the estimation errors are less than 2° in self-occlusion. For the tank LECRERC model, as long as the upper and side planes of the target are not completely occluded, even though the occlusion reaches 80%, the pose angles can be estimated with the estimation error less than 2.5°. Moreover, the proposed method is robust to noise and effective.
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http://dx.doi.org/10.1364/AO.52.008073DOI Listing
November 2013

Effects of ATPM-ET, a novel κ agonist with partial μ activity, on physical dependence and behavior sensitization in mice.

Acta Pharmacol Sin 2010 Dec 22;31(12):1547-52. Epub 2010 Nov 22.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.

Aim: to investigate the effects of ATPM-ET [(-)-3-N-Ethylaminothiazolo [5,4-b]-N-cyclopropylmethylmorphinan hydrochloride] on physical dependence and behavioral sensitization to morphine in mice.

Methods: the pharmacological profile of ATPM-ET was characterized using competitive binding and GTPγS binding assays. We then examined the antinociceptive effects of ATPM-ET in the hot plate test. Morphine dependence assay and behavioral sensitization assay were used to determine the effect of ATPM-ET on physical dependence and behavior sensitization to morphine in mice.

Results: the binding assay indicated that ATPM-ET ATPM-ET exhibited a high affinity to both κ- and μ-opioid receptors with K(i) values of 0.15 nmol/L and 4.7 nmol/L, respectively, indicating it was a full κ-opioid receptor agonist and a partial μ-opioid receptor agonist. In the hot plate test, ATPM-ET produced a dose-dependent antinociceptive effect, with an ED(50) value of 2.68 (2.34-3.07) mg/kg. Administration of ATPM-ET (1 and 2 mg/kg, sc) prior to naloxone (3.0 mg/kg, sc) injection significantly inhibited withdrawal jumping of mice. In addition, ATPM-ET (1 and 2 mg/kg, sc) also showed a trend toward decreasing morphine withdrawal-induced weight loss. ATPM-ET (1.5 and 3 mg/kg, sc) 15 min before the morphine challenge significantly inhibited the morphine-induced behavior sensitization (P<0.05).

Conclusion: ATPM-ET may have potential as a therapeutic agent for the treatment of drug abuse.
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http://dx.doi.org/10.1038/aps.2010.164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4002947PMC
December 2010

The role of kappa-opioid receptor activation in mediating antinociception and addiction.

Acta Pharmacol Sin 2010 Sep 23;31(9):1065-70. Epub 2010 Aug 23.

Nanjing University of Chinese Medicine, China.

The kappa-opioid receptor (KOR), a member of the opioid receptor family, is widely expressed in the central nervous system and peripheral tissues. Substantial evidence has shown that activation of KOR by agonists and endogenous opioid peptides in vivo may produce a strong analgesic effect that is free from the abuse potential and the adverse side effects of mu-opioid receptor (MOR) agonists, such as morphine. In addition, activation of the KOR has also been shown to exert an inverse effect on morphine-induced adverse actions, such as tolerance, reward, and impairment of learning and memory. Therefore, the KOR has received much attention in the effort to develop alternative analgesics to MOR agonists and agents for the treatment of drug addiction. However, KOR agonists also produce several severe undesirable side effects such as dysphoria, water diuresis, salivation, emesis, and sedation in nonhuman primates, which may limit the clinical utility of KOR agonists for pain and drug abuse treatment. This article will review the role of KOR activation in mediating antinociception and addiction. The possible therapeutic application of kappa-agonists in the treatment of pain and drug addiction is also discussed.
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http://dx.doi.org/10.1038/aps.2010.138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4002313PMC
September 2010

[Minimally invasive treatment of intertrochanteric fractures with locking compression plate in the elderly].

Zhongguo Gu Shang 2010 May;23(5):337-9

Department of Orthopaedics, Xiyuan Hospital, China Academy of Chinese Science Medicine ,Beijing 100091, China.

Objective: To study the effect of minimally invasive treatment with the locking compression plate (LCP) in the treatment of intertrochanteric fractures in the elderly age.

Methods: Twenty-eight cases of intertrochanteric fracture were retrospective studied from August 2007 to January 2009, included 13 males and 15 females with an average age of 78.6 years ranging from 70 to 102 years. All the 28 patients were treated with minimally invasive operations with locking compression plates. The time from injury to operation was ranged from 3 to 8 days (with an average of 4.5 days).

Results: The operation time was from 40 to 90 minutes (with an average of 55 minutes). The average bleeding volume during the operation was 70 ml (from 50 to 150 ml). One patient died during hospital stay. Twenty-five patients were followed up from 6 months to 2 years with an average of 15 months after operation. The fracture healing time was from 10 to 12 weeks (10.4 weeks in average). According to an evaluation standard of HUANG Gong-yi, the results were excellent in 20 cases,good in 4 cases, poor in 1 case.

Conclusion: Minimally invasive approaches with LCP could treat the elder intertrochanteric fractures with the advantages such as minimal invasive, stable fixation and less blood loss.
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May 2010

Adenosine A(1) receptor agonist N(6)-cyclohexyl-adenosine induced phosphorylation of delta opioid receptor and desensitization of its signaling.

Acta Pharmacol Sin 2010 Jul 21;31(7):784-90. Epub 2010 Jun 21.

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.

Aim: To define the effect of adenosine A(1) receptor (A(1)R) on delta opioid receptor (DOR)-mediated signal transduction.

Methods: CHO cells stably expressing HA-tagged A(1)R and DOR-CFP fusion protein were used. The localization of receptors was observed using confocal microscope. DOR-mediated inhibition of adenylyl cyclase was measured using cyclic AMP assay. Western blots were employed to detect the phosphorylation of Akt and the DOR. The effect of A(1)R agonist N(6)-cyclohexyladenosine (CHA) on DOR down-regulation was assessed using radioligand binding assay.

Results: CHA 1 micromol/L time-dependently attenuated DOR agonist [D-Pen(2,5)]enkephalin (DPDPE)-induced inhibition of intracellular cAMP accumulation with a t(1/2)=2.56 (2.09-3.31) h. Pretreatment with 1 micromol/L CHA for 24 h caused a right shift of the dose-response curve of DPDPE-mediated inhibition of cAMP accumulation, with a significant increase in EC(50) but no change in E(max). Pretreatment with 1 micromol/L CHA for 1 h also induced a significant attenuation of DPDPE-stimulated phosphorylation of Akt. Moreover, CHA time-dependently phosphorylated DOR (Ser363), and this effect was inhibited by A(1)R antagonist 1,3-Dipropyl-8-cyclopentylxanthine (DPCPX) but not by DOR antagonist naloxone. However, CHA failed to produce the down-regulation of DOR, as neither receptor affinity (K(d)) nor receptor density (B(max)) of DOR showed significant change after chronic CHA exposure.

Conclusion: Activation of A(1)R by its agonist caused heterologous desensitization of DOR-mediated inhibition of intracellular cAMP accumulation and phosphorylation of Akt. Activation of A(1)R by its agonist also induced heterologous phosphorylation but not down-regulation of DOR.
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http://dx.doi.org/10.1038/aps.2010.70DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007732PMC
July 2010

Chronic morphine treatment impaired hippocampal long-term potentiation and spatial memory via accumulation of extracellular adenosine acting on adenosine A1 receptors.

J Neurosci 2010 Apr;30(14):5058-70

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institute For Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, China.

Chronic exposure to opiates impairs hippocampal long-term potentiation (LTP) and spatial memory, but the underlying mechanisms remain to be elucidated. Given the well known effects of adenosine, an important neuromodulator, on hippocampal neuronal excitability and synaptic plasticity, we investigated the potential effect of changes in adenosine concentrations on chronic morphine treatment-induced impairment of hippocampal CA1 LTP and spatial memory. We found that chronic treatment in mice with either increasing doses (20-100 mg/kg) of morphine for 7 d or equal daily dose (20 mg/kg) of morphine for 12 d led to a significant increase of hippocampal extracellular adenosine concentrations. Importantly, we found that accumulated adenosine contributed to the inhibition of the hippocampal CA1 LTP and impairment of spatial memory retrieval measured in the Morris water maze. Adenosine A(1) receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine significantly reversed chronic morphine-induced impairment of hippocampal CA1 LTP and spatial memory. Likewise, adenosine deaminase, which converts adenosine into the inactive metabolite inosine, restored impaired hippocampal CA1 LTP. We further found that adenosine accumulation was attributable to the alteration of adenosine uptake but not adenosine metabolisms. Bidirectional nucleoside transporters (ENT2) appeared to play a key role in the reduction of adenosine uptake. Changes in PKC-alpha/beta activity were correlated with the attenuation of the ENT2 function in the short-term (2 h) but not in the long-term (7 d) period after the termination of morphine treatment. This study reveals a potential mechanism by which chronic exposure to morphine leads to impairment of both hippocampal LTP and spatial memory.
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http://dx.doi.org/10.1523/JNEUROSCI.0148-10.2010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6632807PMC
April 2010

Paradoxical relationship between RAVE (relative activity versus endocytosis) values of several opioid receptor agonists and their liability to cause dependence.

Acta Pharmacol Sin 2010 Apr 15;31(4):393-8. Epub 2010 Mar 15.

School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.

Aim: To examine the relationship between the RAVE (relative activity versus endocytosis) values of opiate agonists and their dependence liability by studying several potent analgesics with special profiles in the development of physical and psychological dependence.

Methods: The effects of (-)-cis-(3R,4S,2'R) ohmefentanyl (F9202), (+)-cis-(3R,4S,2'S) ohmefentanyl (F9204), dihydroetorphine (DHE) and morphine on [(35)S]GTP gamma S binding, forskolin-stimulated cAMP accumulation, and receptor internalization were studied in CHO cells stably expressing HA-tagged mu-opioid receptors (CHO-HA-MOR). cAMP overshoot in response to the withdrawal of these compound treatments was also tested.

Results: All four agonists exhibited the same rank order of activity in stimulation of [(35)S]GTP gamma S binding, inhibition of adenylyl cyclase (AC) and induction of receptor internalization: DHE>F9204>F9202>morphine. Based on these findings and the previous in vivo analgesic data obtained from our and other laboratories, the RAVE values of the four agonists were calculated. The rank order of RAVE values was morphine>F9202>F9204>DHE. For the induction of cAMP overshoot, the rank order was F9202>or=morphine>F9204>or=DHE.

Conclusion: Taken in combination with previous findings of these compounds' liability to develop dependence, the present study suggests that the agonist with the highest RAVE value seems to have a relatively greater liability to develop psychological dependence relative to the agonist with the lowest RAVE value. However, the RAVE values of these agonists are not correlated with their probability of developing physical dependence or inducing cAMP overshoot, a cellular hallmark of dependence.
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http://dx.doi.org/10.1038/aps.2010.19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007660PMC
April 2010
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