Publications by authors named "Jian-Feng Chen"

167 Publications

ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2-Beclin 1 Complex in Cervical Cancer Ca Ski Cells.

Int J Mol Sci 2021 Nov 14;22(22). Epub 2021 Nov 14.

Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China.

RCE-4, a steroidal saponin isolated from , has been studied previously and has exhibited promising anti-cervical cancer properties by inducing programmed cell death (PCD) of Ca Ski cells. Considering the cancer cells developed various pathways to evade chemotherapy-induced PCD, there is, therefore, an urgent need to further explore the potential mechanisms underlying its actions. The present study focused on targeting the Bcl-2-Beclin 1 complex, which is known as the key regulator of PCD, to deeply elucidate the molecular mechanism of RCE-4 against cervical cancer. The effects of RCE-4 on the Bcl-2-Beclin 1 complex were investigated by using the co-immunoprecipitation assay. In addition, autophagy-related genes (ATG) were also analyzed due to their special roles in PCD. The results demonstrated that RCE-4 inhibited the formation of the Bcl-2-Beclin 1 complex in Ca Ski cells via various pathways, and ATG 4B proteins involved in this process served as a key co-factor. Furthermore, based on the above, the sensitivity of RCE-4 to Ca Ski cells was significantly enhanced by inhibiting the expression of the ATG 4B by applying the ATG 4B siRNA plasmid.
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http://dx.doi.org/10.3390/ijms222212302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8617943PMC
November 2021

Masks for COVID-19.

Adv Sci (Weinh) 2021 Nov 26:e2102189. Epub 2021 Nov 26.

Department of Mechanical Engineering, City University of Hong Kong, Hong Kong, 999077, China.

Sustainable solutions on fabricating and using a face mask to block the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread during this coronavirus pandemic of 2019 (COVID-19) are required as society is directed by the World Health Organization (WHO) toward wearing it, resulting in an increasingly huge demand with over 4 000 000 000 masks used per day globally. Herein, various new mask technologies and advanced materials are reviewed to deal with critical shortages, cross-infection, and secondary transmission risk of masks. A number of countries have used cloth masks and 3D-printed masks as substitutes, whose filtration efficiencies can be improved by using nanofibers or mixing other polymers into them. Since 2020, researchers continue to improve the performance of masks by adding various functionalities, for example using metal nanoparticles and herbal extracts to inactivate pathogens, using graphene to make masks photothermal and superhydrophobic, and using triboelectric nanogenerator (TENG) to prolong mask lifetime. The recent advances in material technology have led to the development of antimicrobial coatings, which are introduced in this review. When incorporated into masks, these advanced materials and technologies can aid in the prevention of secondary transmission of the virus.
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http://dx.doi.org/10.1002/advs.202102189DOI Listing
November 2021

Fabrication of a High-Performance and Reusable Planar Face Mask in Response to the COVID-19 Pandemic.

Engineering (Beijing) 2021 Nov 3. Epub 2021 Nov 3.

State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China.

The coronavirus disease 2019 (COVID-19) pandemic has caused a surge in demand for face masks, with the massive consumption of masks leading to an increase in resource-related and environmental concerns. In this work, we fabricated meltblown polypropylene (mb-PP)-based high-performance planar face masks and investigated the effects of six commonly used disinfection methods and various mask-wearing periods on the reusability of these masks. The results show that, after three cycles of treatment using hot water at 70 °C for 30 min, which is one of the most scalable, user-friendly methods for viral disinfection, the particle filtration efficiency (PFE) of the mask remained almost unchanged. After mask wearing for 24 h and subsequent disinfection using the same treatment procedures, the PFE decreased to 91.3%; the average number of bacterial and fungal colonies was assessed to be 9.2 and 51.6 CFU∙g, respectively; and coliform and pyogenic bacteria were not detected. Both the PFE and the microbial indicators are well above the standard for reusable masks after disinfection. Schlieren photography was then used to assess the capabilities of used and disinfected masks during use; it showed that the masks exhibit a high performance in suppressing the spread of breathed air.
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http://dx.doi.org/10.1016/j.eng.2021.07.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8563499PMC
November 2021

A General Strategy for Instantaneous and Continuous Synthesis of Ultrasmall Metal-Organic Framework Nanoparticles.

Angew Chem Int Ed Engl 2021 Dec 3;60(50):26390-26396. Epub 2021 Nov 3.

State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing, 100029, China.

Ultrasmall metal-organic frameworks (MOFs) may generate unique properties to expand the scope of applications. However, the synthesis is still a great challenge. Herein, we propose a strategy to synthesize ultrasmall MOFs by high gravity technology. With the aid of tremendous intensification of molecular mixing and mass transfer in high-gravity field, six typical MOFs were obtained instantaneously in a continuous way. These samples are monodispersed with sub-5 nm in size, smaller than the previously reported values and even close to the length of one crystal unit cell. As a proof-of-concept, catalytic activity for Knoevenagel reaction can be significantly enhanced using ultrasmall ZIF-8. Conversion time of benzaldehyde was decreased by 94 % or 75 % compared to those using conventional or hierarchically porous ZIF-8. More importantly, this approach is readily scalable with the highest space-time yield for nano-MOFs, which may promote the convenient synthesis and practical applications of ultrasmall MOFs in large-scale.
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http://dx.doi.org/10.1002/anie.202112250DOI Listing
December 2021

Metabolite profiling reveals comprehensive effects of Chaetomium globosum on citrus preservation.

Food Chem 2022 Feb 26;369:130959. Epub 2021 Aug 26.

Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, PR China; Key Laboratory of Functional Yeast, China National Light Industry, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, PR China. Electronic address:

The huge economic loss of citrus fruit after harvest called for safe and efficient preservatives, as chemically synthesized agents threatened the environment and human health. Herein a biocontrol fungus Chaetomium globosum QY-1 near the orchard in riparian area was identified to have antimicrobial, antioxidant and tyrosinase inhibition activity, which meets the requirements of an ideal preservative. Metabolite profiling based on bioassay-guided fractionation was carried out, and eight polyketones were determined by MS and NMR. The most abundant CheA exhibited strong inhibition to Penicillium digitatum, the main pathogen caused citrus fruit rot. Among these metabolites, Epicoccone and Epicoccolide B showed higher antioxidant activity, while Epicoccone and CheA had higher tyrosinase inhibitory activity. All the activities were close to or even better than the positive control (Vc; glutathione; Vc and arbutin; Bellkute), implying that the metabolites of C. globosum had comprehensive effects as natural preservatives.
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http://dx.doi.org/10.1016/j.foodchem.2021.130959DOI Listing
February 2022

Cost-benefit analysis of hepatic resection, radiofrequency ablation and liver transplantation in small hepatocellular carcinoma.

Expert Rev Pharmacoecon Outcomes Res 2021 Oct 14:1-7. Epub 2021 Oct 14.

Department of Hepatobiliary Surgery, Qingdao Hospital of Traditional Chinese Medicine(Qingdao Hiser Hospital), Qingdao, China.

Objective: The aim of the present study was to evaluate the cost-effectiveness ratio of surgical treatment options for small hepatocellular carcinoma (SHC) by using the decision tree model and providing a reference for the clinical therapeutic decisions for SHC.

Methods: The data of 719 cases with SHC in the BCLC 0-A who were treated in the past were collected. The survival duration and treatment cost of patients in each experimental group after hepatic resection (HR), radiofrequency ablation (RFA), and orthotopic liver transplantation (OLT) were statistically analyzed.

Results: For SHC with a diameter of less than 3.0 cm, HR, RFA, and OLT had similar cost-effectiveness ratios. OLT could achieve a longer life expectancy, but it was greatly affected by the dropout rate while waiting for the liver donor. RFA was preferred when the willingness to pay (WTP) < 2,5000 RMB/QALY, OLT was preferred when WTP > 75,000 RMB/QALY, and HR was preferred when WTP was between the two.

Expert Opinion: HR in SHC with OLT had the longest life expectancy, but due to the limitations of organ sources, OLT was the preferred treatment option when the WTP was large enough.
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http://dx.doi.org/10.1080/14737167.2021.1973432DOI Listing
October 2021

[Integrin activation, focal adhesion maturation and tumor metastasis].

Sheng Li Xue Bao 2021 Apr;73(2):151-159

State Key Laboratory of Cell Biology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.

Integrins are a large family of heterodimeric cell adhesion molecules composed of α and β subunits. Through interaction with their specific ligands, integrins mediate cell-cell and cell-extracellular matrix interactions. Via outside-in signaling, integrins can recruit cytoplasmic proteins to their intracellular domains and then cluster into supramolecular structures and trigger downstream signaling. Integrin activation is associated with a global conformation rearrangement from bent to extended in ectodomains and the separation of α and β subunit cytoplasmic domains. During cell migration, integrins regulate the focal adhesion dynamics and transmit forces between the extracellular matrix and the cell cytoskeleton. In tumor microenvironment, integrins on multiple kinds of cells could be activated, which modulates cell migration into tumor and contributes to angiogenesis and tumor metastasis. Here, we review the mechanism of integrin activation, dynamics of focal adhesions during cell migration and tumor metastasis.
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April 2021

Comparison of acute pneumonia caused by SARS-COV-2 and other respiratory viruses in children: a retrospective multi-center cohort study during COVID-19 outbreak.

Mil Med Res 2021 02 16;8(1):13. Epub 2021 Feb 16.

Department of Neonatology, Children's Hospital of Chongqing Medical University/Ministry of Education Key Laboratory of Child/Development and Disorders/National Clinical Research Center for Child Health and Disorders/Chongqing Key Laboratory of Pediatrics, Chongqing, 400014, China.

Background: Until January 18, 2021, coronavirus disease-2019 (COVID-19) has infected more than 93 million individuals and has caused a certain degree of panic. Viral pneumonia caused by common viruses such as respiratory syncytial virus, rhinovirus, human metapneumovirus, human bocavirus, and parainfluenza viruses have been more common in children. However, the incidence of COVID-19 in children was significantly lower than that in adults. The purpose of this study was to describe the clinical manifestations, treatment and outcomes of COVID-19 in children compared with those of other sources of viral pneumonia diagnosed during the COVID-19 outbreak.

Methods: Children with COVID-19 and viral pneumonia admitted to 20 hospitals were enrolled in this retrospective multi-center cohort study. A total of 64 children with COVID-19 were defined as the COVID-19 cohort, of which 40 children who developed pneumonia were defined as the COVID-19 pneumonia cohort. Another 284 children with pneumonia caused by other viruses were defined as the viral pneumonia cohort. The epidemiologic, clinical, and laboratory findings were compared by Kolmogorov-Smirnov test, t-test, Mann-Whitney U test and Contingency table method. Drug usage, immunotherapy, blood transfusion, and need for oxygen support were collected as the treatment indexes. Mortality, intensive care needs and symptomatic duration were collected as the outcome indicators.

Results: Compared with the viral pneumonia cohort, children in the COVID-19 cohort were mostly exposed to family members confirmed to have COVID-19 (53/64 vs. 23/284), were of older median age (6.3 vs. 3.2 years), and had a higher proportion of ground-glass opacity (GGO) on computed tomography (18/40 vs. 0/38, P < 0.001). Children in the COVID-19 pneumonia cohort had a lower proportion of severe cases (1/40 vs. 38/284, P = 0.048), and lower cases with high fever (3/40 vs. 167/284, P < 0.001), requiring intensive care (1/40 vs. 32/284, P < 0.047) and with shorter symptomatic duration (median 5 vs. 8 d, P < 0.001). The proportion of cases with evaluated inflammatory indicators, biochemical indicators related to organ or tissue damage, D-dimer and secondary bacterial infection were lower in the COVID-19 pneumonia cohort than those in the viral pneumonia cohort (P < 0.05). No statistical differences were found in the duration of positive PCR results from pharyngeal swabs in 25 children with COVID-19 who received antiviral drugs (lopinavir-ritonavir, ribavirin, and arbidol) as compared with duration in 39 children without antiviral therapy [median 10 vs. 9 d, P = 0.885].

Conclusion: The symptoms and severity of COVID-19 pneumonia in children were no more severe than those in children with other viral pneumonia. Lopinavir-ritonavir, ribavirin and arbidol do not shorten the duration of positive PCR results from pharyngeal swabs in children with COVID-19. During the COVID-19 outbreak, attention also must be given to children with infection by other pathogens infection.
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http://dx.doi.org/10.1186/s40779-021-00306-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886299PMC
February 2021

Surface Engineering of Titanium Dioxide Nanoparticles for Silicone-Based Transparent Hybrid Films with Ultrahigh Refractive Indexes.

Langmuir 2021 Mar 16;37(8):2707-2713. Epub 2021 Feb 16.

Beijing Advanced Innovation Center for Soft Matter Science and Engineering, State Key Laboratory of Organic Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China.

We report a newly developed surface engineering approach for TiO nanoparticles toward transparent TiO/silicone nanocomposites with high refractive index (RI) values. Zirconate coupling agents are adopted on the TiO nanoparticles for surface passivation and to enhance the dispersibility of the nanoparticles in organic substrates. The modified TiO nanoparticles can be uniformly dispersed in silicone, forming transparent hybrid films with an ultrahigh RI of 2.01. The preparation technique of colloidal TiO and polymer-based nanocomposites is simple and suitable for scalable production, which is promising for expanding the application of TiO materials in photonic devices.
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http://dx.doi.org/10.1021/acs.langmuir.0c03377DOI Listing
March 2021

Transareolar single-port endoscopic thoracic sympathectomy with a flexible endoscope for primary palmar hyperhidrosis: a prospective randomized controlled trial.

Ann Transl Med 2020 Dec;8(24):1659

Department of Thoracic Surgery, Palmar Hyperhidrosis Research Institute, First Affiliated Hospital of Fujian Medical University, Fuzhou, China.

Background: Transareolar single-port endoscopic thoracic sympathectomy (ETS) with a flexible endoscope has rarely been reported. This study assessed the performance of this novel minimally invasive technique for primary palmar hyperhidrosis (PPH).

Methods: From January 2019 to September 2019, 118 males with severe PPH requiring single-port and bilateral ETS were randomly allocated to undergo transareolar ETS using a flexible endoscope (group A, n=58) or transaxillary ETS using a 5 mm thoracoscope (group B, n=60).

Results: Both groups had similar patient characteristics. All procedures were performed successfully, with no mortality or conversion to open surgery. All patients had dry and warm palms immediately after surgery. Compared with group B, group A had a significantly shorter median incision length [5.1 (5.0-5.2) 10.9 (10.8-11.9) mm; P<0.001], and significantly lower median postoperative pain score [1 (1.0-2.0) 3 (3.0-4.0); P<0.001]. There were no differences between the two groups in operative time, palmar temperature increase, and transient postoperative sweating. After complete follow-up, group A had a significantly higher median cosmetic score than group B [4.0 (3.0-4.0) 3.0 (3.0-3.0); P<0.001]. There were no differences between the two groups regarding symptom resolution, compensatory hyperhidrosis, and satisfaction score. No patient reported residual pain or symptom recurrence.

Conclusions: Transareolar single-port ETS with a flexible endoscope is safe, effective, and minimally invasive with a small incision, minimal pain, and excellent cosmetic results. This novel procedure is suitable for routine treatment of PPH in males.
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http://dx.doi.org/10.21037/atm-20-7399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812235PMC
December 2020

Conductive Skeleton-Heterostructure Composites Based on Chrome Shavings for Enhanced Electromagnetic Interference Shielding.

ACS Appl Mater Interfaces 2020 Nov 10;12(47):53076-53087. Epub 2020 Nov 10.

State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, PR China.

Renewable bio-based electromagnetic interference (EMI) shielding materials receive increasing attention undoubtedly. However, there is still a challenge to use raw biomass materials to construct a significant structure through an effortless and environmental route for EMI shielding applications. Herein, for the first time, we demonstrated a hybrid composite of multi-walled carbon nanotube/polypyrrole/chrome-tanned collagen fiber (MWCNT/PPy/CF), which utilized waste chrome shavings as a matrix. X-ray photoelectron spectroscopy reveals that the chromium on the CF has a binding effect on the PPy layer, which endows the tight integration between the CF and PPy layer. After the MWCNT network was loaded on the PPy layer, this ternary structure could provide stable conductive paths and a rich number of polarized interfaces. The MWCNT/PPy/CF composite exhibits superior electrical conductivity (354 ± 52 S/m), higher than PPy/CF (222 ± 38 S/m) and MWCNT/CF (104 ± 11 S/m), owing to the synergy of dual conductive structures. Notably, the shielding effectiveness (SE) value of the MWCNT/PPy/CF composite reaches 30 dB in the X band at a thickness of 0.48 mm. The shielding effectiveness of reflection (SE) (9.1 dB) is similar to that of PPy/CF (8.2 dB), while the shielding effectiveness of absorption (SE) is significantly improved from 15.3 dB (PPy/CF) to 20.4 dB (MWCNT/PPy/CF) due to the additional coverage of the MWCNT network. This indicates the synergy between the MWCNT network and conductive PPy/CF skeleton. This work provided a method to prepare sustainable and low-cost renewable EMI shielding materials using chrome shavings. Meanwhile, this novel structure combining a conductive skeleton and heterostructure can be considered as a potential application in relevant fields.
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http://dx.doi.org/10.1021/acsami.0c14300DOI Listing
November 2020

Can Masks Be Reused After Hot Water Decontamination During the COVID-19 Pandemic?

Engineering (Beijing) 2020 Oct 27;6(10):1115-1121. Epub 2020 Jun 27.

State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China.

Masks have become one of the most indispensable pieces of personal protective equipment and are important strategic products during the coronavirus disease 2019 (COVID-19) pandemic. Due to the huge mask demand-supply gap all over the world, the development of user-friendly technologies and methods is urgently needed to effectively extend the service time of masks. In this article, we report a very simple approach for the decontamination of masks for multiple reuse during the COVID-19 pandemic. Used masks were soaked in hot water at a temperature greater than 56 °C for 30 min, based on a recommended method to kill COVID-19 virus by the National Health Commission of the People's Republic of China. The masks were then dried using an ordinary household hair dryer to recharge the masks with electrostatic charge to recover their filtration function (the so-called "hot water decontamination + charge regeneration" method). Three kinds of typical masks (disposable medical masks, surgical masks, and KN95-grade masks) were treated and tested. The filtration efficiencies of the regenerated masks were almost maintained and met the requirements of the respective standards. These findings should have important implications for the reuse of polypropylene masks during the COVID-19 pandemic. The performance evolution of masks during human wear was further studied, and a company (Zhejiang Runtu Co., Ltd.) applied this method to enable their workers to extend the use of masks. Mask use at the company was reduced from one mask per day per person to one mask every three days per person, and 122 500 masks were saved during the period from 20 February to 30 March 2020. Furthermore, a new method for detection of faulty masks based on the penetrant inspection of fluorescent nanoparticles was established, which may provide scientific guidance and technical methods for the future development of reusable masks, structural optimization, and the formulation of comprehensive performance evaluation standards.
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http://dx.doi.org/10.1016/j.eng.2020.05.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320690PMC
October 2020

Liquid Marbles in Liquid.

Small 2020 09 14;16(37):e2002802. Epub 2020 Aug 14.

Department of Mechanical Engineering, City University of Hong Kong, Hong Kong, 999077, China.

Traditional liquid marbles (LMs), liquid droplets encapsulated by hydrophobic particles at the liquid-gas interface, are restricted by their short lifetime and low heat transfer efficiency. Herein, a new paradigm for LMs immersed in various liquid mediums with massive enhanced heat transfer and spatial recognition is designed; without compromising the structural integrity, the lifetime of the liquid marbles in liquid (LMIL) is extended by ≈1000 times compared to classical LMs in air or naked droplets in organic reagents. The LMIL shows promising reverse structural re-configurability while under external stimuli and maintaining their functionality for a very long period of time (≈weeks). These superior behaviors are further exploited as a miniature reactor with prolonged lifetimes and excellent temperature control, combined with its feasible operation, new opportunities will open up in the advanced chemical and biomedical engineering fields. It is also shown that LMIL can be applied in methylene blue degradation and 3D in-vitro yeast cell cultures. These findings have important implications for real-world use of LMs, with a number of applications in cell culture technology, lab-in-a-drop, polymerization, encapsulation, formulation, and drug delivery.
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http://dx.doi.org/10.1002/smll.202002802DOI Listing
September 2020

Involvement of activin a receptor type 1 (ACVR1) in the pathogenesis of primary focal hyperhidrosis.

Biochem Biophys Res Commun 2020 07 27;528(2):299-304. Epub 2020 May 27.

Department of Thoracic Surgery, Fujian Medical University Union Hospital, No. 29 Xinquan Road, Gulou District, Fuzhou, People's Republic of China. Electronic address:

The pathogenesis of primary focal hyperhidrosis (PFH) is still not clear. PFH is thought to be a genetic disease. Whether activin A receptor type 1 (ACVR1) is involved in the pathogenesis of PFH is unknown. In this study, the expression of ACVR1 in sweat glands of patients with PAH was detected by western blot and immunofluorescence. The primary sweat gland cells obtained from primary axillary hyperhidrosis (PAH) patients were transfected with acvr1 vector. Cell proliferation, apoptosis and cell cycling of gland cells were measured after transfection with acvr1 vector. The mRNA and protein expression of aquaporin 5 (AQP5) and Na:K:2Cl Cotransporter 1 (NKCC1/SLC12A2) were detected. Our data showed that ACVR1 expression in axillary sweat gland tissue of PAH patients was significantly higher than that of normal control group. The function of ACVR1 was further investigated in the gland cells obtained from PAH patients. Compared with NC group, ACVR1 overexpression significantly promoted the proliferation of sweat gland cells and inhibited the apoptosis of sweat gland cells. Meanwhile, ACVR1 overexpression significantly reduced the percentage of cells in G0/G1 and G2/M phases, and increased the percentage of cells in S phase. In addition, ACVR1 overexpression significantly promoted the expression of AQP5 and NKCC1 at both mRNA and protein levels. Together, ACVR1 expression is related to PFH and ACVR1 overexpression can promote the proliferation of sweat gland cells and inhibit apoptosis by promoting the expression of AQP5 and NKCC1.
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http://dx.doi.org/10.1016/j.bbrc.2020.05.052DOI Listing
July 2020

Engineering of Long-Term Stable Transparent Nanoemulsion Using High-Gravity Rotating Packed Bed for Oral Drug Delivery.

Int J Nanomedicine 2020 9;15:2391-2402. Epub 2020 Apr 9.

State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.

Background: Oil-in-water drug nanoemulsion forms drug delivery systems with high oral bioavailability. The conventional fabrication methods of nanoemulsion are low energy emulsification methods and high energy emulsification methods. However, both two methods are not ideal for industrial production. The problem of low energy emulsification methods is the high dosage of surfactant and co-surfactant which has potential biosecurity issues. What is more, high energy emulsification methods have some disadvantages, like the destruction of drug components, the price of equipment and the difficulties of industrial production. Hence, there have been a few commercial drug nanoemulsions so far.

Methods: In this work, we reported a novel method for the fabrication of stable and transparent drug nanoemulsion which contains hydrophilic drug rosuvastatin (ROS) calcium or hydrophobic drug silybinin (SYN) by using high-gravity rotating packed bed (RPB). The drug nanoemulsion was systematically characterized by droplet size, size distribution, stability and in vitro drug release as well as Caco-2 cells permeability.

Results: Compared with the self-emulsification method (SE), high-gravity technology could reduce 75% amount of mixed surfactants. The as-prepared nanoemulsion exhibited a very narrow droplet size distribution with a size of 13.53 ± 0.53 nm and a polydispersity index of 0.073 ± 0.018. Meanwhile, the drug nanoemulsion was physicochemically stable at 25°C and 4°C for one-year storage. Furthermore, both ROS and SYN nanoemulsion displayed higher cell permeability and in vitro dissolution than that of commercial formulations.

Conclusion: These results demonstrate that RPB can be a potential device to facilitate the industrial production of drug nanoemulsion.
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http://dx.doi.org/10.2147/IJN.S238788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154039PMC
August 2020

Classification of induced malaria case in an elimination setting: investigation of transfusion-transmitted malaria cases.

Malar J 2020 Mar 30;19(1):136. Epub 2020 Mar 30.

National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi, 214064, Jiangsu, People's Republic of China.

Background: Since the National Malaria Elimination Action Plan was launched in China in 2010, local malaria transmission has decreased rapidly. Zero indigenous cases were reported since 2017. However, after 2010, the proportion of imported cases in China increased from 45.7% in 2010 to 99.9% in 2016, and almost all provinces of China have reported imported cases in recent years. Prevention of the reintroduction of malaria into China is crucial for the maintenance of its malaria-free status. Hence, it is of utmost importance to correctly identify the source of malaria infections within the country.

Case Introduction And Response: In 2016 and 2017, three laboratory-confirmed cases of malaria caused by Plasmodium falciparum were identified in patients with no previous travel history to endemic areas were reported in Jiangsu Province, China, where malaria due to P. falciparum was eliminated about 30 years ago. These were diagnosed after 41, 31 and 39 days of seeking treatment, respectively, and all of them had received blood transfusions. Further investigations indicated that two of the cases had received blood from foreign students (from Indonesia and Ghana), and the other had received blood from an individual who had worked in Equatorial Guinea. All three blood donors were traced, and found to be carrying asymptomatic P. falciparum infections by microscopic examination and PCR. Furthermore, five polymorphic microsatellite markers (C1M4, C4M62, C13M13, C14M17, and C13M63) were typed and used to link parasites from the donors with those of the transfusion-receiving patients.

Conclusions: Three transfusion-transmitted malaria cases were identified in China, all of which were due to the transfusion of blood donated by individuals who had contracted malaria outside the country. These cases can provide a reference for those faced with similar challenges in malaria case identification and classification in other regions. In addition, a stricter screening policy including the use of appropriate detection methods for malaria parasites should be developed and adopted for blood donation in regions undergoing malaria elimination.
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http://dx.doi.org/10.1186/s12936-020-03203-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106567PMC
March 2020

Separation of micro and sub-micro diamagnetic particles in dual ferrofluid streams based on negative magnetophoresis.

Electrophoresis 2020 06 19;41(10-11):909-916. Epub 2020 Mar 19.

School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian, P. R. China.

In the present study, we numerically demonstrate an approach for separation of micro and sub-micro diamagnetic particles in dual ferrofluid streams based on negative magnetophoresis. The dual streams are constructed by an intermediate sheath flow, after which the negative magnetophoretic force induced by an array of permanent magnets dominates the separation of diamagnetic particles. A simple and efficient numerical model is developed to calculate the motions of particles under the action of magnetic field and flow field. Effects of the average flow velocity, the ratio of sheath fluid flow to sample fluid flow, the number of the magnet pair as well as the position of magnet pair are investigated. The optimal parametric condition for complete separation is obtained through the parametric analysis, and the separation principle is further elucidated by the force analysis. The separation of smaller micro and sub-micro diamagnetic particles is finally demonstrated. This study provides an insight into the negative magnetophoretic phenomenon and guides the fabrication of feasible, low-cost diagnostic devices for sub-micro particle separation.
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http://dx.doi.org/10.1002/elps.202000002DOI Listing
June 2020

Enhanced O-linked Glcnacylation in Crohn's disease promotes intestinal inflammation.

EBioMedicine 2020 Mar 27;53:102693. Epub 2020 Feb 27.

Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, China; College of Biological Sciences, Henan University, Kaifeng, China; Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, United States. Electronic address:

Background: Treatment of Crohn's disease (CD) remains to be a challenge due to limited insights for its pathogenesis. We aimed to determine the role of O-Linked β-N-acetylglucosamine (O-GlcNAc) in the development of CD and evaluate therapeutic effects of O-GlcNAc inhibitors on CD.

Methods: O-GlcNAc in intestinal epithelial tissues of CD, adherent-invasive Escherichia coli (AIEC) LF82-infected cells and mice was determined by immunoblot and immunohistochemistry. AIEC LF82 and dextran sulfate sodium were administrated into C57BL/6 mice for estabolishing inflammatory bowel disease model and for therapeutic study.

Findings: O-GlcNAc was increased in intestinal epithelial tissues of CD patients and AIEC LF82-infected mice. Infection of AIEC LF82 up-regulated the level of UDP-GlcNAc and increased O-GlcNAc in human colon epithelial HCT116 and HT-29 cells. We identified that IKKβ and NF-κB were O-Glycosylated in AIEC LF82-treated cells. Mutations of IKKβ (S733A) and p65 (T352A) abrogated the O-GlcNAc in IKKβ and NF-κB and inhibited AIEC LF82-induced activation of NF-κB. Application of 6-diazO-5-oxO-L-norleucine, an agent that blocks the production of UDP-GlcNAc and inhibits O-GlcNAc, inactivated NF-κB in AIEC LF82-infected cells, enhanced the formation of autophagy, promoted the removal of cell-associated AIEC LF82, alleviated intestinal epithelial inflammation, and improved the survival of the colitis mice.

Interpretation: Intestinal inflammation in CD is associated with increased O-GlcNAc modification, which is required for NF-κB activation and suppression of autophagy. Targeting O-GlcNAc could be an effective therapy for inflammatory bowel disease.

Funding: National Natural Science Foundation of China (Nos. 81573087 and 81772924) and International Cooperation Foundation of Jilin Province (20190701006GH).
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http://dx.doi.org/10.1016/j.ebiom.2020.102693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7047186PMC
March 2020

Therapeutic assessment of fractions of Gastrodiae Rhizoma on chronic atrophic gastritis by H NMR-based metabolomics.

J Ethnopharmacol 2020 May 25;254:112403. Epub 2020 Feb 25.

Center of Molecular Metabolism, Nanjing University of Science and Technology, 200 Xiaolingwei Street, Nanjing, 210094, China. Electronic address:

Ethnopharmacological Relevance: Gastrodiae Rhizoma (GR), a well-known and commonly-used TCM (Traditional Chinese Medicine) for treating headache, dizziness, tetanus, epilepsy, and etc., has been proven to relieve chronic atrophic gastritis (CAG). Due to its complex ingredients, the active fractions responsible for the treatment of CAG remain largely unknown.

Aim Of The Study: To explore the underlying material and interpret its underlying mechanism, the therapeutic effect of extract from different polar parts of Gastrodiae Rhizoma on autoimmune CAG was studied based on the H NMR metabolomics.

Materials And Methods: The rat model of CAG was established by autoimmune method. The modeled CAG rats were then treated with 4 polar parts (T1-4 in descending polarity, corresponding to water, n-butanol, ethyl acetate and petroleum ether extracts, respectively) of Gastrodiae Rhizoma for 21 consecutive days. The stomach and serum samples were collected and then subjected to histopathology observation, biochemical measurement (MDA, SOD, GSH, NO, XOD and pepsin), H NMR metabolic profiling and multivariate/univariate statistical analysis.

Results: The results showed that T1 had the best therapeutic effect, T2 the second, and T3 and T4 the poorest with no obvious therapeutic effect, demonstrating that the effective components of Gastrodiae Rhizoma should be compounds of high polarity. T1 achieved good therapeutic effects due to the anti-inflammatory and anti-oxidant activities, and by rectifying the disturbed energy and amino acid metabolism in CAG model.

Conclusion: This integrated metabolomics approach proved the validity of the therapeutic effect of extract from different polar parts of Gastrodiae Rhizoma on autoimmune CAG, providing new insights into the underlying mechanisms, and demonstrating the feasibility of metabolomics to evaluate efficacy of herbal drug, which is often difficult by traditional means.
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http://dx.doi.org/10.1016/j.jep.2019.112403DOI Listing
May 2020

Effects of a high concentration of hydrogen on neurological function after traumatic brain injury in diabetic rats.

Brain Res 2020 03 8;1730:146651. Epub 2020 Jan 8.

Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical University, 246 XueFu Road, NanGang District, Harbin 150086, People's Republic of China. Electronic address:

Reactive oxygen species, inflammation, and apoptosis are major contributors to secondary injuries that follow traumatic brain injury (TBI) in diabetic patients. Hydrogen (H) can selectively neutralize reactive oxygen species and downregulate inflammatory and apoptotic factors. Therefore, we investigated the effects of inhaled high and low concentrations of hydrogen on neurological function after TBI in diabetic rats and the potential mechanism. We found that the inhalation of high concentrations of H significantly improved outcomes following TBI in diabetic rats. The inhalation of 42% H for one hour per day for 48 h significantly reduced brain edema, decreased the extravasation of sodium fluorescein, and reduced oxidative stress markers (p < 0.05). In addition, the inhalation of a high concentration of H (42% for one hour per day for 7 days) improved neurological deficits (p < 0.05) and reduced the expression of apoptotic protein markers (p < 0.05). However, the inhalation of 3% H did not yield significant effects. These results showed that the inhalation of 42% H can alleviate nerve damage and improve neurological function after TBI in diabetic rats. Therefore, the inhalation of a high concentration of H may be associated with the treatment of traumatic brain injuries.
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http://dx.doi.org/10.1016/j.brainres.2020.146651DOI Listing
March 2020

RCE‑4, a potential anti‑cervical cancer drug isolated from Reineckia carnea, induces autophagy via the dual blockade of PI3K and ERK pathways in cervical cancer CaSki cells.

Int J Mol Med 2020 01 31;45(1):245-254. Epub 2019 Oct 31.

Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang, Hubei 443002, P.R. China.

The steroidal saponin RCE‑4 (1β, 3β, 5β, 25S)‑spirostan‑1, 3‑diol 1‑[α‑L‑rhamnopyranosyl‑(1→2)‑β‑D‑xylopyranoside], isolated from Reineckia carnea, exerts significant anti‑cervical cancer activity by inducing apoptosis. The potential effect of RCE‑4 on proliferation inhibition and autophagy induction has rarely been studied. Therefore, the focus of the present study was to investigate the effects of RCE‑4 on proliferation, and to elucidate the detailed mechanisms involved in autophagy induction in cervical cancer cells. CaSki cells were treated with RCE‑4 or/and autophagy inhibitors, and the effect of RCE‑4 on cellular proliferation was assessed by MTT assay. The pro‑autophagic properties of RCE‑4 were subsequently confirmed using monomeric red fluorescent protein‑green fluorescent protein‑microtubule‑associated proteins 1A/1B light chain 3B (LC3) adenoviruses and CYTO‑ID autophagy assays, and by assessing the accumulation of lipid‑modified LC3 (LC3II). The mechanisms of RCE‑4‑induced autophagy were investigated by western blot analysis. The results demonstrated that inhibiting autophagy significantly promoted RCE‑4‑induced cell death, indicating that autophagy served a protective role following RCE‑4 treatment. In addition, RCE‑4‑induced autophagy was reflected by increased expression levels of the serine/threonine‑protein kinase ULK1, phosphorylated (p)‑ULK1, p‑Beclin‑1 and LC3II, the formation of autophagosomes and autolysosomes, and sequestosome 1 (p62) degradation. Subsequent analysis indicated that RCE‑4 activated the AMP‑activated protein kinase (AMPK) pathway by upregulating AMPK and p‑AMPK, and also inhibited the PI3K and extracellular signal‑regulated kinase (ERK) signaling pathways by downregulating p‑PI3K, p‑Akt, p‑mTOR, Ras, c‑Raf, p‑c‑Raf, dual specificity mitogen‑activated protein kinase kinase (MEK)1/2, p‑MEK1/2 and p‑Erk1/2. Additionally, with increased treatment times RCE‑4 may impair lysosomal cathepsin activity and inhibit autophagy flux by suppressing the expression of AMPK, p‑AMPK, ULK1, p‑ULK1 and p‑Beclin‑1, and upregulating that of p62. These results indicated that the dual RCE‑4‑induced inhibition of the PI3K and ERK pathways may result in a more significant anti‑tumor effect and prevent chemoresistance, compared with the inhibition of either single pathway; furthermore, dual blockade of PI3K and ERK, and the AMPK pathway may be involved in the regulation of autophagy caused by RCE‑4. Taken together, RCE‑4 induced autophagy to protect cancer cells against apoptosis, but AMPK‑mediated autophagy was inhibited in the later stages of RCE‑4 treatment. In addition, autophagy inhibition improved the therapeutic effect of RCE‑4. These data highlight RCE‑4 as a potential candidate for cervical cancer treatment.
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http://dx.doi.org/10.3892/ijmm.2019.4389DOI Listing
January 2020

[Epidemiological investigation of blood transfusion - induced malaria caused by an imported case with infection].

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi 2019 Jul;31(4):439-440

Taizhou Municipal Center for Disease Control and Prevention, Jiangsu Province, Taizhou 225300, China.

Objective: To analyze a case infected with due to blood transfusion in Taizhou City, so as to provide scientific evidence for malaria control.

Methods: The epidemiological investigation of a case with malaria was conducted, and the blood donors'blood samples were collected and detected in laboratory to identify the origin of the infection.

Results: Microscopy detected in the blood samples from a 64-year-old male inpatient with nephropathy, and malaria was diagnosed. The case had no history of travel to Africa or Southeast Asia except blood transfusion, and he had received blood transfusion 14 times during his hospital stay. Detection of blood donors'blood samples showed in the archived blood samples from an Indonesian student studying in China, as revealed by PCR assay, and the student was found to have a history of malaria.

Conclusions: The patient was confirmed to be a case with malaria due to blood transfusion. Screening of malaria should be intensified in blood donors to prevent the development of malaria transmitted by blood transfusion.
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http://dx.doi.org/10.16250/j.32.1374.2018254DOI Listing
July 2019

Tuning the Doping of Europium in Gadolinium Borate Microparticles at Mesoscale Toward Efficient Production of Red Phosphors.

ACS Omega 2019 Sep 28;4(11):14497-14502. Epub 2019 Aug 28.

State Key Laboratory of Organic-Inorganic Composites and Research Center of the Ministry of Education for High Gravity Engineering and Technology, Beijing University of Chemical Technology, Beijing 100029, China.

The ideal product of rare-earth-doped phosphors should have uniform particle size distribution and homogeneous doping ions in each particle, and therefore, intensified micromixing at mesoscale is highly required. In this article, inspired by the concept of "mesoscience", we demonstrate the tuning of Eu doping in GdBO microparticles at mesoscale by a high-gravity-assisted reactive precipitation-coupled calcination process. The high-gravity environment and tiny droplets generated by the high-gravity rotating packed bed (RPB) reactor lead to significant intensification of mass transfer and micromixing, which are beneficial for the homogeneous doping of Eu in the host material during reactive precipitation in liquid solution. Under excitation at 395 nm, the emission spectra of the Eu-doped phosphors exhibit a narrow-band red emission centered at 625 nm and the highest intensity was observed at = 0.2. The RPB products show higher intensity than that of the control group even when the reaction time was shortened to 1/6. After calculation, the quenching in the sample most likely results from dipole-dipole interactions. The chromaticity coordinates for the RPB sample was measured as (0.598, 0.341) with a quantum yield of up to 78.11%, and the phosphors exhibit good thermal stability at 423 K. The phosphors were used as the luminescent materials for light-emitting diodes (LEDs), and the devices showed good performance. Our preliminary study illustrated that high-gravity-assisted approaches are promising for tuning the doping of rare-earth ions in microparticles at mesoscale toward efficient production of phosphors for LEDs.
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http://dx.doi.org/10.1021/acsomega.9b01656DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6740406PMC
September 2019

Surfactant-Free Aqueous Dispersions of Shape- and Size-Controlled Zirconia Colloidal Nanocrystal Clusters with Enhanced Photocatalytic Activity.

Langmuir 2019 Sep 26;35(36):11755-11763. Epub 2019 Aug 26.

Colloidal nanocrystal clusters (CNCs) are formed by clustering nanocrystals into secondary structures, which represent a new class of materials and have attracted considerable attention, owing to their unique collective properties and novel functionalities achieved from the ensembles in addition to the properties of each individual subunit. Here, we design a simple route to prepare aqueous dispersions of highly stable ZrO CNCs with tunable shape and size without modification. ZrO CNCs are composed of many ZrO nanocrystals each with a size of about 7 nm and possess a mesoporous structure. Both cube-like and star-like shapes of CNCs can be achieved by using different alkaline sources, while the size of CNCs can be adjusted by changing the hydrothermal time. The as-prepared aqueous dispersions of ZrO CNCs display an enhanced photocatalytic activity in the degradation of rhodamine B (RhB), compared with ZrO nanodispersions. More interestingly, star-like ZrO CNCs show better photocatalytic degradation properties than those of cube-like counterparts and even commercial P25. Furthermore, ZrO CNCs are easily recycled and can be used for the degradation of a range of dye systems.
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http://dx.doi.org/10.1021/acs.langmuir.9b01757DOI Listing
September 2019

New eremophilane-type sesquiterpenes and maleimide-bearing compounds from Carpesium abrotanoides L.

Fitoterapia 2019 Oct 6;138:104294. Epub 2019 Aug 6.

Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China. Electronic address:

Two new eremophilane-type sesquiterpenes, carperemophilanes A and B (1-2), three new maleimide-bearing compounds, carpesiumaleimides A-C (3-5), along with a known sesquiterpene, carabrol (6), were isolated from the ethanol extract of Carpesium abrotanoides L. Their structures were elucidated by analysis of their NMR and MS data as well as by comparison with the literature. The absolute configuration of carperemophilane A (1) was determined by single-crystal X-ray diffraction analysis. All isolated compounds (1-6) were evaluated in vitro for cytotoxicity against two human cancer cell lines MDA-MB-231 and HGC-27 using the MTT method. Compounds 1, 2 and 6 showed cytotoxic activities with IC values ranging from 7.45 to 37.35 μM.
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http://dx.doi.org/10.1016/j.fitote.2019.104294DOI Listing
October 2019

Potential hepatoxicity risk of the shell of Herpetospermum caudigerum Wall in rats based on H-NMR metabonomics.

J Pharm Biomed Anal 2019 Nov 1;176:112800. Epub 2019 Aug 1.

Center of Molecular Metabolism, Nanjing University of Science and Technology, 200 Xiaolingwei Street, Nanjing, 210094, China. Electronic address:

The Herpetospermum caudigerum Wall (HCW) is a traditional Tibetan medicine and is widely used in clinical practice. However, the shell of the HCW (SHCW) has rarely been studied, and some researchers have suggested that the SHCW may be toxic. Therefore, in this study, SHCW was administered to rats at two doses (0.1 and 0.33 g/kg) once a day for 21 days. The hepatic stimuli induced by SHCW in rats were investigated for the first time by H-NMR-based metabolomics combined with histopathological observation and biochemical detection. Histopathological sections showed a certain degree of hepatocyte edema and hepatic sinus congestion in the liver tissue of the rats in the drug-administered group. Serum biochemical indicators revealed a significant increase in ALT, AST, and MDA, and a significant decrease in SOD. Metabolomic results showed that the metabolites in rats were changed after gavage administration of extracts from SHCW. By multivariate statistical analysis and univariate analysis, it was found that SHCW could cause the disorder of energy metabolism, oxidative stress and amino acid metabolism in rats, leading to liver damage. This comprehensive metabolomics approach demonstrates its ability to describe the global metabolic state of an organism and provides a powerful and viable tool for exploring drug-induced toxicity or side effects.
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http://dx.doi.org/10.1016/j.jpba.2019.112800DOI Listing
November 2019

Coated colloidosomes as novel drug delivery carriers.

Expert Opin Drug Deliv 2019 Sep 8;16(9):903-906. Epub 2019 Aug 8.

Department of Chemical Engineering and Biotechnology, BP Institute, University of Cambridge, Madingley Rise , Cambridge , UK.

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http://dx.doi.org/10.1080/17425247.2019.1652594DOI Listing
September 2019

[Effect of subsoiling depths on soil physical characters and sugarcane yield].

Ying Yong Sheng Tai Xue Bao 2019 Feb;30(2):405-412

Lituo Agricultural Services Co. Ltd, Laibin 546100, Guangxi, China.

We investigated the physical properties of the plough soil and the components of sugarcane yield in the depth of mechanized subsoiling of sugarcane field, along with the clarification on the specific soil location and obstacle factors of subsoiling, with the aim to provide scientific basis for the construction of a good sugarcane cultivation layer and the development of soil improvement strategies. Three depths of subsoiling operation (35, 40 and 45 cm) were set up, with nosubsoiling as control. Soil physical properties, including compactness, bulk density, water content, porosity, three-phase volume ratio, and yield components and cane yield of sugarcane in the fields were investigated. The results showed that subsoiling depth was significantly correlated with the soil structure characteristics and the improvement of sugarcane yield in sugarcane field. Subsoiling broke down the plow bottom, significantly reduced soil compaction, bulk density, and the corresponding penetration resistance and shear strength during mechanical operation, especially for the above factors in 20-30 cm soil layer, with positive consequences for sugarcane yield. Moreover, subsoiling significantly increased the liquid volume rate of the soil layer within 30 cm and soil moisture storage capacity, and thus significantly improved the water index of the 10-30 cm soil layer. The 10-30 cm soil layer was the location for the most significant effect of subsoiling on the improvement of solid volume rate in the plough soil. The effective stem number, plant height, cane yield and sucrose content of sugarcane were significantly promoted by subsoiling. In view of the common equipment level in the sugarcane planting area, we suggested that the operating depth standard of mechanized subsoiling should not be less than 40 cm.
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http://dx.doi.org/10.13287/j.1001-9332.201902.010DOI Listing
February 2019

[Evaluation of implementation effect of schistosomiasis control program in Jiangsu Province from 2010 to 2015].

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi 2019 Jan;30(6):615-618

Key Laboratory of National Health Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Public Health Research Center at Jiangnan University, Wuxi 214064, China.

Objective: To evaluate the actual effect of the schistosomiasis control program in Jiangsu Province from 2010 to 2015.

Methods: A total of 67 schistosomiasis-endemic counties in 10 cities were selected, and a combination of retrospective investigation and on-site investigation was adopted to collect and record the epidemic data of the counties from 2010 to 2015, and a retrospective survey database of epidemic situation was established. The effects of integrated control strategies with both snail control and infection source control were evaluated.

Results: From 2010 to 2015, 2 465 911 persons who lived in endemic areas were detected for schistosomiasis, with 16 974 positive cases of blood examinations, and 8 positive cases of fecal examinations. Totally 5 145 people with advanced schistosomiasis were treated and 40 460 people with the history of schistosome cercarial-infested water contact received the expanded chemotherapy. A total of 127 636 cattle raised in the endemic areas were detected, and 51 619 cattle (head-times) with the history of cercarial-infested water contact also received the expanded chemotherapy. The area with snails control by molluscicides was 18 604.84 hm. By the end of 2015, schistosomeinfected snails had not been found and there was no zoological schistosome infection for 5 consecutive years, and in addition, there had been no acute schistosome-infected persons for 6 consecutive years in the whole province. The area with snails dropped to 1 977.18 hm, with a decreasing rate of 55.24% compared with that in 2010.

Conclusions: After the implementation of the plan for the prevention and control of schistosomiasis in Jiangsu Province (2010-2015), the prevention and control of schistosomiasis has achieved remarkable effects and realized the goal of the plan.
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http://dx.doi.org/10.16250/j.32.1374.2018209DOI Listing
January 2019

[Performance Evaluation of the CLIA and ECLIA for Anti-TP Screening in Blood Donors].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Feb;27(1):260-265

Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.E-mail:

Objective: To evaluate the performance of the chemiluminescence immune assay (CLIA) and the electro-chemiluminescence immuneoassay(ECLIA) for Treponemapallidum antibody(anti-TP) screening in blood donors.

Methods: The sero-panel samples from NCCL were tested with ELISA, CLIA and ECLIA assays synchronously to evaluate their performances respectively.

Results: The sensitivity and the negative predictive value of the CLIA were 100%, which were the same as one kind of ELISA, and better than the other ELISA; The specificity of the CLIA was 88.46%, the accuracy rate was 97.02%, the positive predictive value was 96.13%, which were higher than both ELISA. Due to the significant interference of sample heat inactivation in ECLIA detection, the result can not demonstrate the true performance of ECLIA in this study. The preliminary result was as follows: the sensitivity was 98.93%, the negative predictive value was 96.75%, and the accuracy rate, specificity and positive predictive value of ECLIA were 97.02%, 91.54% and 97.10% respectively.

Conclusion: Compared with ELISA, the CLIA has higher sensitivity and specificity and can be used for Treponemal antibody screening in blood bank. Unfortunately, the data in this study cannot come to a conclusion for ECLIA and needs more testing.
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http://dx.doi.org/10.7534/j.issn.1009-2137.2019.01.042DOI Listing
February 2019
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