Publications by authors named "Jian Zuo"

273 Publications

Prestin amplifies cardiac motor functions.

Cell Rep 2021 May;35(5):109097

Division of Cardiovascular Medicine, University of California, Davis, Davis, CA 95616, USA; Department of Veterans Affairs, VA Northern California Health Care System, Mather, CA 95655, USA. Electronic address:

Cardiac cells generate and amplify force in the context of cardiac load, yet the membranous sheath enclosing the muscle fibers-the sarcolemma-does not experience displacement. That the sarcolemma sustains beat-to-beat pressure changes without experiencing significant distortion is a muscle-contraction paradox. Here, we report that an elastic element-the motor protein prestin (Slc26a5)-serves to amplify actin-myosin force generation in mouse and human cardiac myocytes, accounting partly for the nonlinear capacitance of cardiomyocytes. The functional significance of prestin is underpinned by significant alterations of cardiac contractility in Prestin-knockout mice. Prestin was previously considered exclusive to the inner ear's outer hair cells; however, our results show that prestin serves a broader cellular motor function.
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http://dx.doi.org/10.1016/j.celrep.2021.109097DOI Listing
May 2021

Status quo of illegal dumping research: Way forward.

J Environ Manage 2021 Apr 22;290:112601. Epub 2021 Apr 22.

School of Architecture and Built Environment, The University of Adelaide, SA, 5001, Australia. Electronic address:

Due to the rapid social and economic development, the past decades have witnessed the improvement of human being's quality of life and the speedy development of the construction industry. Meanwhile, the illegal dumping of solid waste has presented a significant issue. By using the method of systematic review, this study critically examined the literature related to illegal dumping that were published since 1990, and analyzed the current status and future trends of related research. Results show that the current studies on illegal dumping mainly focus on four perspectives: environmental science and toxicology, economics, management, and the use of emerging technologies. This critical review revealed that although the issue of illegal dumping has been widely recognized in recent years, some questions remain unanswered. Therefore, a future research agenda is proposed. These include: (1) Identifying the migration of pollutants in the food chain during the illegal dumping; (2) Implementing targeted treatment of illegal dumping pollutants; (3) Improving the stakeholder decision analysis model; (4) Expanding the scope of research on stakeholders of illegal dumping; (5) Formulating an unified evaluation standard for the related costs of illegal dumping; (6) Strengthening the evaluation of the interaction effects of influencing factors; (7) Comparing the effects of different types of factors; (8) the exploration of other influencing factors; (9) Analyzing illegal dumping by combining big data with the amount of solid waste; (10) Combining with monitoring to analyze the illegal dumping of household waste.
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http://dx.doi.org/10.1016/j.jenvman.2021.112601DOI Listing
April 2021

Clinical profiles and outcomes of acute type A aortic dissection and intramural hematoma in the current era: lessons from the first registry of aortic dissection in China.

Chin Med J (Engl) 2021 Apr 13;134(8):927-934. Epub 2021 Apr 13.

Department of Cardiovascular Surgery, Xijing Hospital, the Fourth Military Medical University, Xi'an, Shaanxi 710032, China.

Background: Acute type A aortic dissection (ATAAD) and acute type A intramural hematoma (ATAIMH) are life-threatening diseases with high mortality. To better understand their clinical features in the Chinese population, we analyzed the data from the first Registry of Aortic Dissection in China (Sino-RAD) to promote the understanding and management of the diseases.

Methods: All patients with ATAAD and ATAIMH enrolled in Sino-RAD from January 1, 2012 to December 31, 2016 were involved. The data of patients' selection, history, symptoms, management, outcomes, and postoperation complications were analyzed in the study. The continuous variables were compared using the Student's t test for normal distributions and the Mann-Whitney U test for non-normal distributions. Categorical variables were compared using the Chi-square test or Fisher exact test.

Results: A total of 1582 patients with ATAAD and 130 patients with ATAIMH were included. The mean age of all patients was 48.4 years. Patients with ATAAD were significantly younger than patients with ATAIMH (48.9 years vs. 55.6 years, P < 0.001). For the total cohort, males were dominant, but the male ratio of patients with ATAAD was significantly higher compared to those with ATAIMH (P = 0.01). The time range from the onset of symptom to hospitalization was 2.0 days. More patients of ATAIMH had hypertension than that of ATAAD (82.3% vs. 67.6%, P < 0.05). Chest and back pain were the most common clinical symptoms. Computerized tomography (CT) was the most common initial diagnostic imaging modality. 84.7% received surgical treatment and in-hospital mortality was 5.3%. Patients with ATAAD mainly received surgical treatment (89.6%), while most patients with ATAIMH received medical treatment (39.2%) or endovascular repair (35.4%).

Conclusions: Our study suggests that doctors should comprehensively use clinical examination and genetic background screening for patients with ATAAD and ATAIMH and further shorten the time range from symptoms onset to intervention, achieving early diagnosis and treatment, thereby reducing the mortality rate of patients with aortic dissection in China. We should standardize the procedures of aortic dissection treatment and improve people's understanding. Meanwhile, the curing and transferring efficiency should also be improved.
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http://dx.doi.org/10.1097/CM9.0000000000001459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078379PMC
April 2021

Assessment models and dynamic variables for dynamic life cycle assessment of buildings: a review.

Environ Sci Pollut Res Int 2021 Mar 30. Epub 2021 Mar 30.

Department of Construction and Real Estate, School of Civil Engineering, Southeast University, Nanjing, 211189, China.

Life cycle assessment (LCA) is widely used to quantify the environmental performance of buildings. Recently, the potential temporal variations in the lifetime of buildings and their influences on assessment results have attracted considerable attention. Dynamic LCA (DLCA) is an emerging research topic. This study provides an overview of the current scenario of DLCA studies in the building field. A literature survey was conducted by searching through scientific literature databases; 48 articles met the inclusion criteria. Eleven dynamic variables as well as their addressing approaches were summarized and analyzed. A few typical dynamic assessment models were synthesized and compared to present the methodology progress. Finally, considering the existing limitations, a few research directions were recommended: setting cutoff criteria for dynamic variables, developing a dynamic database, and considering the interactions between dynamic variables. The analyses in this study indicate that research on the DLCA of buildings needs interdisciplinary cooperation. This review promotes in-depth understanding about DLCA research of buildings and offers valuable implications for environmental practice. The highlighted future research directions facilitate further explorations in this research area.
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http://dx.doi.org/10.1007/s11356-021-13614-1DOI Listing
March 2021

Conflict or consensus? Stakeholders' willingness to participate in China's Sponge City program.

Sci Total Environ 2021 May 20;769:145250. Epub 2021 Jan 20.

Beijing Capital Co., LTD, Beijing 100044, China.

In 2015, China initiated the Sponge City Program (SCP), an integrated urban stormwater management strategy, to mitigate the paradoxical challenges of urban flooding and water shortages. Very few studies have attempted to examine the willingness to participate of multi-level stakeholders in the case of storm-water management initiatives. To address this gap in the existing body of knowledge, this study took Guyuan city, Ningxia Province, a Stage 2 pilot city located in the arid northwest region of the People's Republic of China, as a case study. Members of three key stakeholder groups were surveyed, i.e., government, commercial entities and communities, and a total of 687 valid questionnaires were obtained in the SCP. Via a logistic regression model, the results showed that: (1) the stakeholders reached a consensus during the participation process that they were motivated by personal benefits and expected to improve water usage through the participatory process; (2) the government represented the public interest by prioritizing the overall improvement of the urban water environment and improving standards of living by contributing to the program; (3) the commercial entities prioritized the acquisition of knowledge and technical issues relevant to their business operations, and expressed concerns about how these operations might be affected by stakeholder participation; (4) both the commercial and community groups were more willing to participate if they perceived that the SCP would affect their daily lives, and the general public were willing to participate for multiple reasons. This study will help to guide future studies to continuously explore the diverse factors that influence the stakeholder participation of diverse stakeholders. The findings can also benefit the design of future projects with a view to enhancing stakeholder participation. Recognizing the quantifiable benefits of the SCP, this paper demonstrates how the evaluation of diverse stakeholders' priorities and the assessment of the drivers for their willingness to participate can further benefit the implementation of sustainable urban water initiatives, as in the case of this megaproject, and their enduring success.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145250DOI Listing
May 2021

PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.

Mol Cell 2021 04 3;81(7):1425-1438.e10. Epub 2021 Mar 3.

School of Life Science and Technology, Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China; Department of Neurology, Affiliated ZhongDa Hospital, Institute of Neuropsychiatry, Southeast University, Nanjing, Jiangsu 210009, China. Electronic address:

Eukaryotic elongation factor 2 (eEF2) mediates translocation of peptidyl-tRNA from the ribosomal A site to the P site to promote translational elongation. Its phosphorylation on Thr56 by its single known kinase eEF2K inactivates it and inhibits translational elongation. Extensive studies have revealed that different signal cascades modulate eEF2K activity, but whether additional factors regulate phosphorylation of eEF2 remains unclear. Here, we find that the X chromosome-linked intellectual disability protein polyglutamine-binding protein 1 (PQBP1) specifically binds to non-phosphorylated eEF2 and suppresses eEF2K-mediated phosphorylation at Thr56. Loss of PQBP1 significantly reduces general protein synthesis by suppressing translational elongation. Moreover, we show that PQBP1 regulates hippocampal metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) and mGluR-LTD-associated behaviors by suppressing eEF2K-mediated phosphorylation. Our results identify PQBP1 as a novel regulator in translational elongation and mGluR-LTD, and this newly revealed regulator in the eEF2K/eEF2 pathway is also an excellent therapeutic target for various disease conditions, such as neural diseases, virus infection, and cancer.
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http://dx.doi.org/10.1016/j.molcel.2021.01.032DOI Listing
April 2021

-Derived Xanthones Protected Joints from Degradation in Male Rats with Collagen-Induced Arthritis by Regulating PPAR-γ Signaling.

J Inflamm Res 2021 16;14:395-411. Epub 2021 Feb 16.

School of Pharmacy, Anhui College of Traditional Chinese Medicine, Wuhu, 241000, Anhui, People's Republic of China.

Background: The bark of Hassk. is traditionally used for treating inflammatory diseases and bone fractures in China. We have previously validated the xanthone-enriched fraction (XRF) of with anti-rheumatic potentials, but mechanism underlying the joints protective effects is still largely unknown.

Materials And Methods: The male rats with collagen-induced arthritis (CIA) were treated with XRF. The therapeutic efficacy of XRF was evaluated by arthritis score changes, morphological observation of paws, histological examinations and serological analyses. Protein expression in tissues and cells was investigated by either immunohistochemical or immunoblotting methods, while levels of mRNA expression were investigated by RT-qPCR. Metabolites in serum were detected by LC-MS approach. The joints homogenates were used for analyzing possible targeted genes by genome microarray analyses.

Results: Treatment with XRF and methotrexate (MTX) led to significant decrease in arthritis scores, and alleviated deformation of paws in CIA rats. In addition, XRF and MTX reduced circulating TNF-α, IL-1β and IL-17α in the serum and down-regulated TLR4/NF-κB and JNK pathways in joints of CIA rats. Compared to MTX, XRF-loading microemulsion significantly protected joints, which was accompanied by dramatic decrease in MMP3. Differential genes-based KEGG enrichment and metabolomics analysis suggested that XRF reduced fatty acids biosynthesis by regulating PPAR-γ signaling. -derived 1,7-dihydroxy-3,4-dimethoxyxanthone (XAN) up-regulated PPAR-γ expression in macrophages, but suppressed it in pre-adipocytes in vitro, which was synchronized with SIRT1 changes. Adiponectin production and SCD-1 expression in pre-adipocytes were also decreased. Aside the direct inhibition on MMP3 expression in synovioblast, the presence of XAN in macrophages-pre-adipocytes co-culture system further reinforced this effect.

Conclusion: This study revealed the joint protective  advantages of the bioactive fraction from in CIA rats over MTX, and demonstrated that -derived xanthones suppressed the erosive nature of synovioblast acquired under inflammatory circumstances by regulating PPAR-γ signaling-controlled metabolism-immunity feedback.
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http://dx.doi.org/10.2147/JIR.S295957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896782PMC
February 2021

A comprehensive review on green buildings research: bibliometric analysis during 1998-2018.

Environ Sci Pollut Res Int 2021 Feb 16. Epub 2021 Feb 16.

School of Environmental Science and Engineering, Tianjin University, No. 135 Yaguan Road, Tianjin, 300350, China.

Buildings account for nearly 2/5ths of global energy expenditure. Due to this figure, the 90s witnessed the rise of green buildings (GBs) that were designed with the purpose of lowering the demand for energy, water, and materials resources while enhancing environmental protection efforts and human well-being over time. This paper examines recent studies and technologies related to the design, construction, and overall operation of GBs and determines potential future research directions in this area of study. This global review of green building development in the last two decades is conducted through bibliometric analysis on the Web of Science, via the Science Citation Index and Social Sciences Citation Index databases. Publication performance, countries' characteristics, and identification of key areas of green building development and popular technologies were conducted via social network analysis, big data method, and S-curve predictions. A total of 5246 articles were evaluated on the basis of subject categories, journals' performance, general publication outputs, and other publication characteristics. Further analysis was made on dominant issues through keyword co-occurrence, green building technologies by patent analysis, and S-curve predictions. The USA, China, and the UK are ranked the top three countries where the majority of publications come from. Australia and China had the closest relationship in the global network cooperation. Global trends of the top 5 countries showed different country characteristics. China had a steady and consistent growth in green building publications each year. The total publications on different cities had a high correlation with cities' GDP by Baidu Search Index. Also, barriers and contradictions such as cost, occupant comfort, and energy consumption were discussed in developed and developing countries. Green buildings, sustainability, and energy efficiency were the top three hotspots identified through the whole research period by the cluster analysis. Additionally, green building energy technologies, including building structures, materials, and energy systems, were the most prevalent technologies of interest determined by the Derwent Innovations Index prediction analysis. This review reveals hotspots and emerging trends in green building research and development and suggests routes for future research. Bibliometric analysis, combined with other useful tools, can quantitatively measure research activities from the past and present, thus bridging the historical gap and predicting the future of green building development.
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http://dx.doi.org/10.1007/s11356-021-12739-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886307PMC
February 2021

Long-term dynamic durability test datasets for single proton exchange membrane fuel cell.

Data Brief 2021 Apr 18;35:106775. Epub 2021 Jan 18.

Clean Energy Automotive Engineering Center and School of Automotive Studies, Tongji University, Shanghai 201804, China.

This dataset collects the long-term dynamic durability test data and the polarization characterization test data used in our research article [1]. The dynamic durability test and the polarization characterization test of a single proton exchange membrane fuel cell (PEMFC) are all performed on the Greenlight 20 test station. The European harmonized test protocol is adapted to construct the fuel cell dynamic load test cycle (FC-DLC) used in this work. The overall durability test is composed of 3076 FC-DLC cycles, around 1008 h. To access the degradation information of the test fuel cell, the polarization characterization tests are performed periodically during the durability test. In this work, the characterizations were performed at time: 0, 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1000 h. During the test period, G20 test station records all measured data, includes the dynamic load durability test dataset and the polarization test dataset. The output voltage degradation trend as well as the polarization curves are plotted and described in this work. This dataset provides the possibilities to study the degradation phenomenon of fuel cell operating by dynamic load cycles, moreover, this dataset can be directly used to various prediction models build for fuel cells.
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http://dx.doi.org/10.1016/j.dib.2021.106775DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851326PMC
April 2021

Characterization of quinoxaline derivatives for protection against iatrogenically induced hearing loss.

JCI Insight 2021 Mar 8;6(5). Epub 2021 Mar 8.

Hair cell loss is the leading cause of hearing and balance disorders in humans. It can be caused by many factors, including noise, aging, and therapeutic agents. Previous studies have shown the therapeutic potential of quinoxaline against drug-induced ototoxicity. Here, we screened a library of 68 quinoxaline derivatives for protection against aminoglycoside-induced damage of hair cells from the zebrafish lateral line. We identified quinoxaline-5-carboxylic acid (Qx28) as the best quinoxaline derivative that provides robust protection against both aminoglycosides and cisplatin in zebrafish and mouse cochlear explants. FM1-43 and aminoglycoside uptake, as well as antibiotic efficacy studies, revealed that Qx28 is neither blocking the mechanotransduction channels nor interfering with aminoglycoside antibacterial activity, suggesting that it may be protecting the hair cells by directly counteracting the ototoxin's mechanism of action. Only when animals were incubated with higher doses of Qx28 did we observe a partial blockage of the mechanotransduction channels. Finally, we assessed the regulation of the NF-κB pathway in vitro in mouse embryonic fibroblasts and in vivo in zebrafish larvae. Those studies showed that Qx28 protects hair cells by blocking NF-κB canonical pathway activation. Thus, Qx28 is a promising and versatile otoprotectant that can act across different species and toxins.
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http://dx.doi.org/10.1172/jci.insight.141561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021103PMC
March 2021

The Role of α7nAChR-Mediated Cholinergic Anti-inflammatory Pathway in Immune Cells.

Inflammation 2021 Jan 6. Epub 2021 Jan 6.

Department of Traditional Chinese Medicine, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital), Wuhu, 241000, China.

Alpha 7 nicotinic acetylcholine receptor (α7nAChR) is widely distributed in the nervous and non-cholinergic immune systems. It is necessary for the cholinergic transmitter to participate in the regulation of inflammatory response and is the key element of cholinergic anti-inflammatory pathway (CAP). Because of the profound impact of CAP on the immune system, α7nAChR is considered as a potential therapeutic target for the treatment of inflammatory diseases. Available evidences confirmed that manipulation of CAP by activating α7nAChR with either endogenous acetylcholine (ACh) or cholinergic agonists can substantially alleviate inflammatory responses both in vivo and in vitro. However, the mechanism through which CAP curbs the excessive pro-inflammatory responses and maintains immune homeostasis is not fully understood. Obtained clues suggest that the crosstalk between CAP and classical inflammatory pathways is the key to elucidate the anti-inflammatory mechanism, and the impacts of CAP activation in α7nAChR-expressing immune cells are the foundation of the immunoregulatory property. In this article, we review and update the knowledge concerning the progresses of α7nAChR-based CAP, including α7nAChR properties, signal transductions, interactions with classic immune pathways, and immunoregulatory functions in different immune cells. Certain critical issues to be addressed are also highlighted. By providing a panoramic view of α7nAChR, the summarized evidences will pave the way for the development of novel anti-inflammatory reagents and strategy and inspire further researches.
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http://dx.doi.org/10.1007/s10753-020-01396-6DOI Listing
January 2021

BRAF inhibition protects against hearing loss in mice.

Sci Adv 2020 Dec 2;6(49). Epub 2020 Dec 2.

Department of Pharmacology and Neuroscience, School of Medicine, Creighton University, Omaha, NE 68178, USA.

Hearing loss caused by noise, aging, antibiotics, and chemotherapy affects 10% of the world population, yet there are no Food and Drug Administration (FDA)-approved drugs to prevent it. Here, we screened 162 small-molecule kinase-specific inhibitors for reduction of cisplatin toxicity in an inner ear cell line and identified dabrafenib (TAFINLAR), a BRAF kinase inhibitor FDA-approved for cancer treatment. Dabrafenib and six additional kinase inhibitors in the BRAF/MEK/ERK cellular pathway mitigated cisplatin-induced hair cell death in the cell line and mouse cochlear explants. In adult mice, oral delivery of dabrafenib repressed ERK phosphorylation in cochlear cells, and protected from cisplatin- and noise-induced hearing loss. Full protection was achieved in mice with co-treatment with oral AZD5438, a CDK2 kinase inhibitor. Our study explores a previously unidentified cellular pathway and molecular target BRAF kinase for otoprotection and may advance dabrafenib into clinics to benefit patients with cisplatin- and noise-induced ototoxicity.
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http://dx.doi.org/10.1126/sciadv.abd0561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821884PMC
December 2020

Electron image contrast analysis of mosaicity in rutile nanocrystals using direct electron detection.

Acta Crystallogr A Found Adv 2020 Nov 23;76(Pt 6):687-697. Epub 2020 Oct 23.

Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 W. Green Street, Illinois 61801, USA.

Direct electron detection provides high detective quantum efficiency, significantly improved point spread function and fast read-out which have revolutionized the field of cryogenic electron microscopy. However, these benefits for high-resolution electron microscopy (HREM) are much less exploited, especially for in situ study where major impacts on crystallographic structural studies could be made. By using direct detection in electron counting mode, rutile nanocrystals have been imaged at high temperature inside an environmental transmission electron microscope. The improvements in image contrast are quantified by comparison with a charge-coupled device (CCD) camera and by image matching with simulations using an automated approach based on template matching. Together, these approaches enable a direct measurement of 3D shape and mosaicity (∼1°) of a vacuum-reduced TiO nanocrystal about 50 nm in size. Thus, this work demonstrates the possibility of quantitative HREM image analysis based on direct electron detection.
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http://dx.doi.org/10.1107/S2053273320011055DOI Listing
November 2020

Xanthones from antagonized the antirheumatic effects of methotrexate by promoting its secretion into urine.

Expert Opin Drug Metab Toxicol 2021 Feb 12;17(2):241-250. Epub 2020 Nov 12.

Key Laboratory of Non-coding RNA Transformation Research of Anhui Higher Education Institution, Wannan Medical College , Wuhu, Anhui, China.

Background: This study was designed to characterize the interaction between Hassk. derived xanthones and methotrexate (MTX).

Methods: Collagen-induced arthritis (CIA) was induced in rats, which were treated with MTX, a xanthone-rich fraction (XRF), or MTX+XRF by gavage for 30 days. Clinical efficacy was assessed based on arthritis scores, serological analysis, and histological examination. Protein expression was investigated by either immunohistochemical or immunoblotting methods. MTX concentrations were determined by HPLC or LC-MS methods. Obtained results were further validated by assays using 1,7-dihydroxy-3,4-dimethoxyxanthone and HEK 293 T cells.

Results: XRF antagonized the antirheumatic effects of MTX , suggested by higher levels of proinflammatory cytokines, and severer swelling and deformation of joints in CIA rats in the MTX+XRF group compared with MTX monotherapy. XRF reduced MTX concentration in plasma and promoted its excretion into urine. As a result, XRF attenuated MTX-induced edema of the proximal tubule. Furthermore, XRF restored the decreased expression of organic anion transporter three (OAT3), which accounts for MTX secretion in the kidney. Consistently, 1,7-dihydroxy-3,4-dimethoxyxanthone promoted the cellular intake of MTX by increasing OTA3 expression.

Conclusion: It is suggested that the combined use of with MTX should be optimized to avoid the antagonistic effects and improve the safety of the MTX regimen.
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http://dx.doi.org/10.1080/17425255.2021.1843634DOI Listing
February 2021

Aldh inhibitor restores auditory function in a mouse model of human deafness.

PLoS Genet 2020 09 24;16(9):e1009040. Epub 2020 Sep 24.

Department of Otorhinolaryngology, Provincial Key Discipline of the affiliated Drum Tower Hospital of Nanjing University and Model Animal Research Center, MOE Key Laboratory of Model Animal for Disease Studies, School of Medicine, Nanjing University, Nanjing, China.

Genetic hearing loss is a common health problem with no effective therapy currently available. DFNA15, caused by mutations of the transcription factor POU4F3, is one of the most common forms of autosomal dominant non-syndromic deafness. In this study, we established a novel mouse model of the human DFNA15 deafness, with a Pou4f3 gene mutation (Pou4f3Δ) identical to that found in a familial case of DFNA15. The Pou4f3(Δ/+) mice suffered progressive deafness in a similar manner to the DFNA15 patients. Hair cells in the Pou4f3(Δ/+) cochlea displayed significant stereociliary and mitochondrial pathologies, with apparent loss of outer hair cells. Progression of hearing and outer hair cell loss of the Pou4f3(Δ/+) mice was significantly modified by other genetic and environmental factors. Using Pou4f3(-/+) heterozygous knockout mice, we also showed that DFNA15 is likely caused by haploinsufficiency of the Pou4f3 gene. Importantly, inhibition of retinoic acid signaling by the aldehyde dehydrogenase (Aldh) and retinoic acid receptor inhibitors promoted Pou4f3 expression in the cochlear tissue and suppressed the progression of hearing loss in the mutant mice. These data demonstrate Pou4f3 haploinsufficiency as the main underlying cause of human DFNA15 deafness and highlight the therapeutic potential of Aldh inhibitors for treatment of progressive hearing loss.
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http://dx.doi.org/10.1371/journal.pgen.1009040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7553308PMC
September 2020

The nexus of water-energy-food in China's tourism industry.

Resour Conserv Recycl 2021 Jan 14;164:105157. Epub 2020 Sep 14.

School of Architecture & Built Environment, Entrepreneurship, Commercialization and Innovation Centre (ECIC), The University of Adelaide, Adelaide 5005, Australia.

The tourism industry contributes significantly to the growth of the global economy and is considered to be strongly associated with a large amounts of water and energy consumption. In this study, the tourism water footprint (TWF) and the tourism energy footprint (TEF) of 138 sectors were investigated to examine the water-energy-food (W-E-F) nexus in the Chinese tourism industry from 2012 to 2017 by developing the water- and energy-based environmentally extended input-output analysis with the tourism satellite account. This study revealed that the W-E-F supply groups consumed total 15,556 million m of water and 4,964 million tce of energy to support the Chinese tourism industry. The largest contributor to the total TWF is the indirect water use from the food supply group (65%), while the largest proportion of total TEF is contributed by the direct energy use from 11 tourism direct sectors (63%), most especially the air transport sector. A much larger growth of the tourism industry was observed in 2017 compared to that of 2012. The structure decomposition analysis revealed that the growth of the overall water and energy consumption of China tourism is mainly driven by the growth of the total tourism expenditure, i.e. the scale effect. It is the same case for the food supply group associated with the Chinese tourism industry. In contrast, the contribution of the changes to the tourism expenditure composition is relatively low. Furthermore, the growth in water and energy consumption can be offset effectively by reducing the water and energy use coefficient and adjusting the economic production structure of tourism and its associated food supply group. In sum, the food supply and air transport sectors play a crucial role in the water-energy-food nexus of the tourism industry. Therefore, in the future, focus should be placed on improving the water and energy use efficiency of these sectors as well as enhancing their production structures.
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http://dx.doi.org/10.1016/j.resconrec.2020.105157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7487940PMC
January 2021

The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells.

Sci Rep 2020 09 16;10(1):15167. Epub 2020 Sep 16.

Department of Biomedical Science, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE, 68178, USA.

Cells of the immune system are present in the adult cochlea and respond to damage caused by noise exposure. However, the types of immune cells involved and their locations within the cochlea are unclear. We used flow cytometry and immunostaining to reveal the heterogeneity of the immune cells in the cochlea and validated the presence of immune cell gene expression by analyzing existing single-cell RNA-sequencing (scRNAseq) data. We demonstrate that cell types of both the innate and adaptive immune system are present in the cochlea. In response to noise damage, immune cells increase in number. B, T, NK, and myeloid cells (macrophages and neutrophils) are the predominant immune cells present. Interestingly, immune cells appear to respond to noise damage by infiltrating the organ of Corti. Our studies highlight the need to further understand the role of these immune cells within the cochlea after noise exposure.
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http://dx.doi.org/10.1038/s41598-020-72181-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7495466PMC
September 2020

Activation of cholinergic anti-inflammatory pathway involved in therapeutic actions of α-mangostin on lipopolysaccharide-induced acute lung injury in rats.

Int J Immunopathol Pharmacol 2020 Jan-Dec;34:2058738420954941

Department of Traditional Chinese Medicine, Yijishan Hospital of Wannan Medical College, Wuhu, China.

Introduction: Alpha-mangostin (MAN) possesses a wide variety of pharmacological effects. In this study, we investigated its effect on cholinergic anti-inflammatory pathway (CAP), and tested if CAP regulation was involved in the therapeutic action on acute lung injury (ALI).

Methods: Male Sprague Dawley rats were pre-treated with MAN (40 mg/kg) for 3 days and ALI was induced with an intraperitoneal injection of lipopolysaccharide (LPS). Certain rats received monolateral vagotomy or sham surgery. The effects on inflammatory reactions and relevant pathways in ALI rats or LPS pre-treated RAW 264.7 cells were investigated by histological, immunohistochemical, immunoblotting, RT-qPCR, and immunofluorescence assays, while levels of proinflammatory cytokines, acetylcholine (Ach) and the enzymatic activity of acetylcholinesterase (AchE) were determined by corresponding quantitative kits.

Results: Oral administration of MAN reduced the severity of ALI, while vagotomy surgery antagonized this effect. MAN restored the decline in α7 nicotinic acetylcholine receptor (α7nAchR) in the lungs of ALI rats, and promoted the expression of α7nAchR and choline acetyltransferase (CHAT) in RAW 264.7 cells. Although AchE expression was barely affected by MAN at 5 μg/ml, its catalytic activity was reduced by almost 95%. Extracellular rather than intracellular Ach was notably raised shortly after MAN treatment. Furthermore, MAN at 5 μg/ml effectively inhibited LPS-induced increase in phosphorylation and nucleus translocation of p65 subunit, and secretion of TNF-α and IL-1β, which was then offset by methyllycaconitine citrate hydrate.

Conclusion: MAN activated CAP by increasing peripheral Ach and up-regulating α7nAchR expression, which eventually led to NF-κB inhibition and remission of acute inflammations.
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http://dx.doi.org/10.1177/2058738420954941DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485160PMC
September 2020

Spatial spillover effects of environmental regulations on air pollution: Evidence from urban agglomerations in China.

J Environ Manage 2020 Oct 17;272:110998. Epub 2020 Jul 17.

School of Architecture & Built Environment, Entrepreneurship, Commercialisation and Innovation Centre (ECIC), The University of Adelaide, SA, 5005, Australia.

Environmental regulations affects the environmental quality of not only local areas but also surrounding regions. It remains unknown whether the effect of environmental regulations on the surrounding regions is free riding or pollution shelter. Based on the data from 2006 to 2018, the spatial correlation of PM in Beijing-Tianjin-Hebei (BTH), Yangtze River Delta (YRD) and Pearl River Delta (PRD) urban agglomerations in China was examined in this study. In addition, the spatial spillover effects of environmental regulation on PM concentrations were explored while the socio-economic driving factors of the heterogeneity of pollution spillover were identified via SDM based STIRPAT framework. Results showed that the characteristics of PM concentrations spatial correlations varies from one urban agglomeration to another. This study revealed that the air pollution is affected by not only local environmental regulations, but also regulations implemented in surrounding cities. The PM concentration of BTH, YRD and PRD increased by 0.76, 0.147 and 0.109 for each unit increase in environmental regulation of surrounding cities, respectively. In fact, cities with loose regulation become the pollution shelters. The spatial spillover effects offset the improvement effects of local environmental regulations on the air quality. Furthermore, the comparison amongst three urban agglomerations showed that the spatial spillover effects of PM concentration in BTH and YRD are higher than that of PRD. This is attributed to differences in industrial structure, population density, economic development, FDI and geographical location. Therefore, the spatial spillover effects should be taken into consideration and joint regulation should be strengthened to address air pollution issues in urban aggregations.
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http://dx.doi.org/10.1016/j.jenvman.2020.110998DOI Listing
October 2020

Economic transition and industrial sulfur dioxide emissions in the Chinese economy.

Sci Total Environ 2020 Nov 12;744:140826. Epub 2020 Jul 12.

School of Architecture & Built Environment, The University of Adelaide, SA 5005, Australia.

China transitioned into "new normal phase" during 2007 to 2017, shifting from pursuing rapid GDP growth to a win-win state of economic development and environmental improvement. Using the input-output (IO) table for 2007-2012 and the latest IO table for 2012-2017, an IO model of China's industrial SO emissions is established and structural decomposition analysis (SDA) is applied to examine the changes in industrial SO emissions resulting from this economic transition. Five influencing factors (emission intensity, production technology, final demand expenditure, final demand structure and economic scale) are taken into consideration. The analysis shows that emission intensity and economic scale are the most influential factors on SO emissions. Emission intensity reduced SO emissions by 16,560,886 t in 2012-2017 whilst economic scale increased SO emissions by 473,490 t. Compared with the period 2007-2012, the contribution rate of emission intensity increased from -82.3% to -189.2%, while that of economic scale decreased from 131.8% to 54.1%. The total contributions of 5 factors to SO reduction increased from -5,249,417 t to -12,783,248 t, and the contribution rate increased from -24.8% to -146%. China's energy conservation and emission reduction has achieved remarkable results between 2007 and 2017. In "new normal phase", the slowing of China economic growth, the transition of economic development, industrial structural adjustment and rational consumption habits have had significant effects in reducing environmental pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140826DOI Listing
November 2020

Individualized Prediction of Overall Survival for Primary Intramedullary Spinal Cord Grade II/III Ependymoma.

World Neurosurg 2020 11 16;143:e149-e156. Epub 2020 Jul 16.

Department of Othopedics, Huangshi Central Hospital, Affiliated Hospital Polytechnic University, Huangshi, China.

Background: The purpose of this study was to develop and validate a nomogram to predict overall survival (OS) for adult patients with primary intramedullary spinal cord grade II/III ependymoma (PISCGE). We also elucidated the effectiveness of postoperative radiotherapy for this disease.

Methods: Clinical data of patients with PISCGE between 1988 and 2015 were collected from the Surveillance, Epidemiology, and End Results (SEER) registry database. The independent prognostic factors were identified using univariate and multivariate Cox analyses. The nomogram was established from the results of the multivariate Cox analysis. We also use some methods to verify the superiority of the prediction model. The effectiveness of postoperative radiotherapy for PISCGE was assessed through coarsened exact matching and survival analyses.

Results: Multivariate Cox analysis revealed that sex, age, surgical treatment, tumor grade, and marital status were independent prognostic factors of OS. The nomogram model was established based on these factors and validated internally. Calibration plots based on bootstrap resampling validation showed good consistency between the nomogram prediction and actual observation. This model also exhibited favorable discrimination characteristics. A risk classification system based on a nomogram was established to promote risk stratification of PISCGE and optimize clinical management. Moreover, we found no association between radiation treatment and the OS for these patients (P > 0.05).

Conclusions: We built the first nomogram model and risk classification system for PISCGE patients. Our model accurately estimated the individual OS probability of these patients, and proposes different treatment approaches for patients based on the risk classification system. Furthermore, from our findings, radiotherapy confers no survival advantage to these patients.
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http://dx.doi.org/10.1016/j.wneu.2020.07.049DOI Listing
November 2020

Information system with multiple data layer approach to select the C&D waste landfilling infrastructure.

Environ Sci Pollut Res Int 2020 Nov 6;27(31):38788-38804. Epub 2020 Jul 6.

School of Architecture and Built Environment, The University of Adelaide, Adelaide, SA, 5001, Australia.

Landfilling is a critical method in managing massive generated C&D waste, and the appropriate selection of C&D waste landfill sites can reduce the impacts of landfilling. This study proposes an approach combined F-AHP and GIS to select suitable C&D waste landfills. The proposed model considers multiple factors from environmental, social and economic aspects. A case study of Shenzhen, China, is undertaken to showcase the implementation of the proposed model. It is found that about 25 million m of land has the potential to be used for C&D waste landfills in the study case, but the actual usable land is limited as some lands are too small for a landfill site. The study contributes to the waste management discipline as it provides an improved framework for selecting a landfill site. Besides, the landfill site selection procedure and results have practical implications for urban planning.
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http://dx.doi.org/10.1007/s11356-020-09951-2DOI Listing
November 2020

Activation of Cholinergic Anti-Inflammatory Pathway in Peripheral Immune Cells Involved in Therapeutic Actions of α-Mangostin on Collagen-Induced Arthritis in Rats.

Drug Des Devel Ther 2020 22;14:1983-1993. Epub 2020 May 22.

Department of Traditional Chinese Medicine, Yijishan Hospital of Wannan Medical College, Wuhu 241000, People's Republic of China.

Background: Studies have shown that α-mangostin (MG) could exert anti-rheumatic effects in vivo by restoring immunity homeostasis, and have indicated that activation of the choline anti-inflammatory pathway (CAP) may contribute to this immunomodulatory property. The current study was designed to further investigate the effects of MG on the CAP in peripheral immune cells and clarify its relevance to the potential anti-rheumatic actions.

Methods: The catalytic activity of acetylcholinesterase (AChE) and expression of α7-nicotinic cholinergic receptor (α7nAChR) in peripheral blood mononuclear cells (PBMCs) from rats with collagen-induced arthritis (CIA) or human volunteers were evaluated after MG treatment. Consequent influences on the immune environment were assessed by flow cytometry and ELISA analyses. Indirect effects on joints resulting from these immune changes were studied in a co-culture system comprised of fibroblast-like synoviocytes (FLSs) and PBMCs.

Results: MG promoted α7nAChR expression in PBMCs both in vivo and in vitro, and inhibited the enzymatic activity of AChE simultaneously. Activation of the CAP was accompanied by a significant decrease in Th17 cells (CD4IL-17A), while no obvious changes concerning the distribution of other T-cell subsets were noticed upon MG treatment. Meanwhile, MG decreased the secretion of TNF-α and IL-1β under inflammatory conditions. PBMCs from MG-treated CIA rats lost the potential to stimulate NF-κB activation and pro-inflammatory cytokine production of FLSs in the co-culture system.

Conclusion: Overall, the evidence suggested that MG can improve the peripheral immune milieu in CIA rats by suppressing Th17-cell differentiation through CAP activation, and achieve remission of inflammation mediated by FLSs.
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http://dx.doi.org/10.2147/DDDT.S249865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250306PMC
March 2021

1,7-Dihydroxy-3,4-Dimethoxyxanthone Inhibits Lipopolysaccharide-Induced Inflammation in RAW264.7 Macrophages by Suppressing TLR4/NF-κB Signaling Cascades.

Inflammation 2020 Oct;43(5):1821-1831

Yijishan Hospital of Wannan Medical College, Wuhu, 241000, China.

Securidaca inappendiculata Hassk. is a traditional Chinese anti-rheumatic herbal medicine native to southern China. In this study, we identified a possible TLR4 inhibitor from this plant. General effects of its xanthone-rich fraction (XRF) on inflammation in vitro were investigated by immunoblotting experiments performed on lipopolysaccharides (LPS)-treated RAW264.7 cells, and the possible ligand of TLR4 within was screened out by analyzing chemical composition differences of the XRF containing cell culture medium under different inflammatory circumstances. The interaction between ligand and TLR4 was validated by cellular thermal shift assay (CETSA) and molecular docking simulation, and TLR4/NF-κB pathway status was investigated by immunoprecipitation, ELISA, immunofluorescence, dual-luciferase reporter, and immunoblotting experiments. Treatment with XRF resulted in significant decrease in p-p65 and p-JNK, and the signal accounting for 1,7-dihydroxy-3,4-dimethoxyxanthone (XAN) at 12.5 min with mass of 289.29 was greatly decreased in XRF containing medium after LPS stimulus because of enhanced interaction with increased TLR4. CETSA and molecular docking simulation demonstrated that XAN could bind to TLR4 directly on a smooth region adjacent to its contact interface with MD-2. XAN treatment inhibited the dimerization of TLR4 and transcriptional activity of NF-κB in HEK293T cells and decreased p65 accumulation in nucleus and pro-inflammatory cytokines production in RAW264.7 cells receiving LPS treatment. Overall evidences suggest that XAN could be a selective TLR4 inhibitor with potent anti-inflammatory effects. Also, it indicated that xanthone derivatives could have promising clinical application in many immune-mediated inflammations by acting as TLR4 inhibitors.
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http://dx.doi.org/10.1007/s10753-020-01256-3DOI Listing
October 2020

Key policies to the development of construction and demolition waste recycling industry in China.

Waste Manag 2020 May 27;108:137-143. Epub 2020 Apr 27.

School of Public Management, South China Agricultural University, Guangzhou 510642, China. Electronic address:

There is a growing need to recycle construction and demolition waste (CDW) in order to treat the huge amount of CDW in many metropolises of China. However, the CDW recycling industry is still in its initial stage and developed unevenly across various areas of China. In spite of some qualitative discussions, the quantitative analysis of crucial policies to the development of CDW recycling industry was overlooked. Through literature review, nine influential policy instruments were identified in term of three categories, i.e. control and command policy, market-based policy and information-based policy. The stepwise regression analysis was employed to explore the relationships between the influential policies and the development level of CDW recycling industry in 52 sample Chinese cities. The results demonstrated that Green Product Label, Charge and Tax and Technical Standards had statistically significant association with the development of CDW recycling industry in sample cities. In the surveyed cities, Charge or Tax was the most common policy tool (84.6%), but Green Product Label (7.7%) and Technological standards (11.5%) were rather less frequently employed. According to the results, Green Product Label and Technical Standards should be given higher priority. In addition, landfill charge should be introduced as a fundamental impetus. These results provide directions for other cities to facilitate the development of their CDW recycling industry.
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http://dx.doi.org/10.1016/j.wasman.2020.04.016DOI Listing
May 2020

Life cycle assessment of ultra-low treatment for steel industry sintering flue gas emissions.

Sci Total Environ 2020 Jul 2;725:138292. Epub 2020 Apr 2.

School of Architecture & Built Environment, The University of Adelaide, SA 5005, Australia.

The largest contributor to pollutant emissions is the sintering process in steel industry. Ultra-low emission policy for the Chinese steel industry states that emission concentrations of particulate matter, SO and NO should not exceed 10, 35 and 50 mg/m respectively. The emission concentrations of the steel industry are the same as the ultra-low emission policy for the coal-fired power industry, but the pollutant control technologies of the two industries are different. Life cycle assessment method is applied to analyze the latest ultra-low treatment process for sintering flue gas emissions which includes electrostatic precipitation, ozone oxidation, wet desulfurization, wet denitration, condensation dehumidification and wet electrostatic precipitation. Following this novel ultra-low emission treatment, the concentrations of particulate matter, SO, NO, and PCDDs in the sintering flue gas decreased very significantly, attaining the new emission standard. With 1 ton of sinter as the functional unit and "cradle to gate" as the system boundary, the environmental impact of the process is 0.1811 and the total economic cost is 172.79 RMB, of which internal cost is 34.64 RMB and external cost is 138.15 RMB. The main environmental impacts result from applying the wet denitration and ozone oxidation processes. Sodium sulfite in the wet denitration process, and electricity and liquid oxygen in the ozone oxidation process are the key inputs that cause environmental impact. These findings are useful for a further optimization of the ultra-low emissions process from both the environmental and economic perspective, which is applicable in other regions of the world.
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http://dx.doi.org/10.1016/j.scitotenv.2020.138292DOI Listing
July 2020

Bioactive fractions from Securidaca inappendiculata alleviated collagen-induced arthritis in rats by regulating metabolism-related signaling.

Kaohsiung J Med Sci 2020 Jul 18;36(7):523-534. Epub 2020 Mar 18.

Department of Electronic Science, Xiamen University, Xiamen, China.

Securidaca inappendiculata is a xanthone rich medicinal plant that has been used in the treatment of inflammation and autoimmune diseases like rheumatoid arthritis (RA) for centuries; however, the material base and mechanism of action responsible for its anti-arthritis effect still remains elusive. The objective of this study is to evaluate the therapeutic effects of xanthone-enriched extract of the plant against collagen-induced arthritis (CIA) in rats and explore the underlying mechanisms. The xanthone-deprived fraction (XDF) and xanthone-rich fraction (XRF) were obtained by using a resin adsorption coupled with acid-base treatment method, and their chemical composition difference was characterized by UPLC-MS/MS analysis. Effects of the two on CIA were analyzed using radiographic, histological, and immunohistochemical analyses. The results indicated that XRF alleviated joint structures destructions with the higher efficacy than XDF, and decreased levels of TNF-α, IL-6, and anti-cyclic citrullinated peptide antibody in CIA rats significantly. Furthermore, XRF inhibited nicotinamide phosphoribosyl transferase (NAMPT) mediated fat biosynthesis and utilization indicated by clinical evidences and metabonomics analysis, which thereby disrupted energy-metabolism feedback. In addition, Toll-like Receptor 4 and High Mobility Group Protein 1 expressions were downregulated in XRF-treated CIA rats. Collective evidences suggest NAMPT could be an ideal target for RA treatments and reveal a novel antirheumatic mechanism of S. inappendiculata by regulating NAMPT controlled fat metabolism.
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http://dx.doi.org/10.1002/kjm2.12205DOI Listing
July 2020

Integrated assessment of the environmental and economic effects of "coal-to-gas conversion" project in rural areas of northern China.

Environ Sci Pollut Res Int 2020 May 10;27(13):14503-14514. Epub 2020 Feb 10.

School of Architecture & Built Environment, The University of Adelaide, Adelaide, SA, 5005, Australia.

Northern China suffers from serious air pollution especially in winter, much of which derives from solid fuel used for domestic heating in rural areas. In order to reduce pollution emissions in the heating season, the Chinese government has introduced a "coal-to-gas conversion" policy, promoting a switch to natural gas which is much cleaner than the coal normally used for winter heating. The "coal-to-gas conversion" project will cover more than 1.8 billion m of heated built floor area and affect more than 12 million heat users in Beijing, Tianjin, Hebei, and the surrounding areas. Life cycle assessment and life cycle cost methods are applied to compare and analyze the environmental impact and economic cost of household energy usage for the whole year under 5 scenarios before and after "coal-to-gas conversion." In the three scenarios after "coal-to-gas conversion," the environmental impact decreases by around 50% while the total economic cost increases by around 80%. Particulate emissions responsible for air pollution are considerably reduced with accompanying benefits for human health, though significant, but reduced, impacts on freshwater and marine ecotoxicity remain. Improving thermal efficiency through natural gas utilization, implementing an energy-saving retrofit of rural housing, and promoting straw utilization yield benefits for people and the environment in rural areas of northern China.
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http://dx.doi.org/10.1007/s11356-020-08004-yDOI Listing
May 2020

α-Mangostin reduced the viability of A594 cells in vitro by provoking ROS production through downregulation of NAMPT/NAD.

Cell Stress Chaperones 2020 01 2;25(1):163-172. Epub 2020 Jan 2.

Yijishan Hospital, Wannan Medical College, Wuhu, 241000, China.

α-Mangostin (MAN) is a bioactive compound isolated from the inedible pericarp of a tropical fruit mangosteen (Garcinia mangostana Linn). It exhibits notable therapeutic potentials on lung cancers, but the underlying mechanisms are still largely unknown. This study was designed to further explore the mechanisms involved in cytotoxicity of MAN on A549 cells. Apoptosis and cell cycle distribution were analyzed by flow cytometry methods. The fluorescent probes DCFH-DA and JC-1 were used to assess the intracellular reactive oxidative species (ROS) and mitochondrial membrane potential statuses, respectively. The regulation of MAN on relevant pathways was investigated by immunoblotting assays. The results obtained indicated that MAN caused significant apoptosis and cell cycle arrest in A549 cells, which eventually resulted in inhibition on cell proliferation in vitro. All these phenomena were synchronized with escalated oxidative stress and downregulation of nicotinamide phosphoribosyltransferase/nicotinamide adenine dinucleotide (NAMPT/NAD). Supplementation with nicotinamide mononucleotide (NMN) and N-acetylcysteine (NAC) efficiently eased MAN-induced ROS accumulation, and potently antagonized MAN-elicited apoptosis and cell cycle arrest. The pro-apoptotic effect of MAN was further confirmed by increased expressions of cleaved caspase 3, 6, 7, and 9, and its effect on cell cycle progression was validated by the altered expressions of p-p38, p-p53, CDK4, and cyclin D1. The immunoblotting assays also demonstrated that NAC/NMN effectively restored these molecular changes elicited by MAN treatment. Collectively, this study revealed a unique anti-tumor mechanism of MAN by provoking ROS production through downregulation of NAMPT/NAD signaling and further validated MAN as a potential therapeutic reagent for lung cancer treatment.
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http://dx.doi.org/10.1007/s12192-019-01063-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6985413PMC
January 2020

Public Perception towards Waste-to-Energy as a Waste Management Strategy: A Case from Shandong, China.

Int J Environ Res Public Health 2019 08 20;16(16). Epub 2019 Aug 20.

School of Architecture & Built Environment, The University of Adelaide, Adelaide 5005, Australia.

Municipal solid waste (MSW) is posing great challenge for most countries in the world, which can cause severe negative impacts to the environment and human health. Waste-to-energy has great potential in China because of its technological maturity and policy support at the national level. However, there are significant conflicts between the huge market demand and strong public opposition. It is imperative to examine the public perception of waste-to-energy, especially for developing countries where a large number of projects are under construction or have been approved. The public perception of waste-to-energy was carried out by a questionnaire survey in this research. A total of 650 questionnaires were distributed and 629 questionnaires were returned, with a response rate of 96.8%. The results show that the public showed general concern in regard to environmental issues. Respondents had an overall positive attitude towards waste-to-energy, but it varied according to the demographic details of residents, such as age, education, and income. Recognition level of the benefits was higher than the concern of associated risks. Multiple linear regression shows that awareness of environmental issues had no impact on public attitude towards waste-to-energy, while public awareness and perceived benefits had notable positive impacts. Perceived risks had a positive correlation with public attitude. In order to promote the development of MSW incinerators, the government should make more publicity efforts. Rural residents, people over 50 years old, and people with low education and low income are the major groups which should be focused on to enhance the public perception. The findings provide a theoretical and practical reference for enhancing the social acceptance of waste-to-energy development.
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http://dx.doi.org/10.3390/ijerph16162997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720593PMC
August 2019