Publications by authors named "Jian Zou"

394 Publications

Live leech in the trachea: an unexpected cause of haemoptysis.

Br J Hosp Med (Lond) 2022 07 11;83(7). Epub 2022 Jul 11.

Department of Otorhinolaryngology-Head and Neck Surgery, West China Hospital of Sichuan University, Chengdu, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12968/hmed.2022.0010DOI Listing
July 2022

The Prognostic Prediction Value of Positive Lymph Nodes Numbers for the Hypopharyngeal Squamous Cell Carcinoma.

Front Med (Lausanne) 2022 4;9:898483. Epub 2022 Jul 4.

Department of Otolaryngology Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu, China.

Background: The current American Joint Committee on Cancer (AJCC) system only considered the importance of the size and laterality of lymph nodes while not the positive lymph node number (PLNN) for hypopharyngeal squamous cell carcinoma (HPSCC).

Methods: A total of 973 patients with HPSCC from the Surveillance, Epidemiology, and End Results database (2004-2015) were identified. Univariate and multivariate Cox regression analyses were used to evaluate the prognostic effects. We applied six Cox regression models to compare the survival prognostic values of PLNN and AJCC systems.

Results: Positive lymph node number showed a significant association with overall survival (OS) and cancer-specific survival (CSS) ( < 0.001) in univariate and multivariable analyses. The increased PLNN of HPSCC gave rise to poor OS and CSS. The survival model incorporating a composite of PLNN and TNM classification (C-index for OS:0.682, C-index for CSS:0.702) performed better than other models.

Conclusions: A positive lymph node number could serve as a survival predictor for patients with HPSCC and a complement to enhance the prognostic assessment effects of TNM cancer staging systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2022.898483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9291443PMC
July 2022

SHF Acts as a Novel Tumor Suppressor in Glioblastoma Multiforme by Disrupting STAT3 Dimerization.

Adv Sci (Weinh) 2022 Jul 17:e2200169. Epub 2022 Jul 17.

Department of Laboratory Medicine, Wuxi People's Hospital of Nanjing Medical University, Wuxi, Jiangsu, 214023, China.

Sustained activation of signal transducer and activator of transcription 3 (STAT3) is a critical contributor in tumorigenesis and chemoresistance, thus making it an attractive cancer therapeutic target. Here, SH2 domain-containing adapter protein F (SHF) is identified as a tumor suppressor in glioblastoma Multiforme (GBM) and its negative regulation of STAT3 activity is characterized. Mechanically, SHF selectively binds and inhibits acetylated STAT3 dimerization without affecting STAT3 phosphorylation or acetylation. Additionally, by blocking STAT3-DNMT1 (DNA Methyltransferase 1) interaction, SHF relieves methylation of tumor suppressor genes. The SH2 domain is documented to be essential for SHF's actions on STAT3, and almost entirely replaces the functions of SHF on STAT3 independently. Moreover, the peptide C16 a peptide derived from the STAT3-binding sites of SHF inhibits STAT3 dimerization and STAT3/DNMT1 interaction, and achieves remarkable growth inhibition in GBM cells in vitro and in vivo. These findings strongly identify targeting of the SHF/STAT3 interaction as a promising strategy for developing an optimal STAT3 inhibitor and provide early evidence of the potential clinical efficacy of STAT3 inhibitors such as C16 in GBM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/advs.202200169DOI Listing
July 2022

The application of the spot the difference teaching method in clinical skills training for residents.

BMC Med Educ 2022 Jul 14;22(1):542. Epub 2022 Jul 14.

Department of Otolaryngology Head & Neck Surgery, West China Hospital, Sichuan University, 37 E Guoxue Alley, Chengdu, Sichuan, 610041, People's Republic of China.

Background: Clinical skill training (CST) is indispensable for first-year surgical residents. It can usually be carried out through video-based flipped learning (FL) within a web-based learning environment. However, we found that residents lack the process of reflection, blindly imitating results in losing interest and passion for learning in the traditional teaching pattern. The teaching method of "spot the difference" (SDTM), which is based on the fundamentals of the popular game of "spot the difference," is designed to improve students' participation and reflective learning during skill training. This study aimed to evaluate this novel educational model's short-term and long-term effectiveness for surgical residents in China.

Methods: First-year residents who required a three-month rotation in the head and neck surgery department were recruited to participate in a series of CSTs. They were randomized into SDTM and traditional FL (control) groups. Clinical skill performance was assessed with validated clinical skill scoring criteria. Evaluations were conducted by comparing the scores that contain departmental rotation skill examinations and the first China medical licensing examination (CMLE) performance on practical skills. In addition, two-way subjective evaluations were also implemented as a reference for the training results. Training effects were assessed using t tests, Mann-Whitney-Wilcoxon tests, chi-square tests, and Cohen' s effect size (d). The Cohen' s d value was considered to be small (<0.2), medium (0.2-0.8), or large (>0.8).

Results: The SDTM group was significantly superior to the control group in terms of after-department skill examination (t=2.179, p<0.05, d=0.5), taking medical history (t=2.665, p<0.05, d=0.59), and CMLE performance on practical skill (t=2.103, p<0.05, d=0.47). The SDTM members rated the curriculum more highly than the control on the items relating to interestingness and participation (p < 0.05) with large effect sizes (d >0.8). There were no significant differences between the two groups on clinical competence (t=0.819, p=0.415, d=0.18), the first-time pass rate for CMLE (χ2 =1.663, p=0.197, d=0.29), and short-term operational skills improvement (t=1.747, p=0.084, d=0.39).

Conclusions: SDTM may be an effective method for enhancing residents' clinical skills, and the effect is significant both short- and long-term. The improvement effect seemed to be more significant in the peer-involved SDTM than training alone. However, despite positive objective results, SDTM still risks student learning burnout.

Trial Registration: ISRCTN registry, ISRCTN10598469 , 02/04/2022,retrospectively registered.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12909-022-03612-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281025PMC
July 2022

A "Square Box"-Structured Triboelectric Nanogenerator for Road Transportation Monitoring.

Polymers (Basel) 2022 Jun 30;14(13). Epub 2022 Jun 30.

Key Laboratory of Advanced Packaging Materials and Technology of Hunan Province, Hunan University of Technology, Zhuzhou 412007, China.

Nowadays, with the rapid development of e-commerce, the transportation of products has become more and more frequent. However, how to monitor the situation of products effectively and conveniently during road transportation is a long-standing problem. In order to meet this problem in practical applications, we fabricated a triboelectric nanogenerator sensor with a "square box" structure (S-TENG) for detecting the vibration suffered by vehicles. Specifically, with the spring installed in the S-TENG as a trigger, the two friction layers can contact and then separate to generate the real-time electrical signals when the S-TENG receives external excitation. The output voltage signals of the S-TENG under different vibration states were tested and the results demonstrated that the peak and zero positions of the open-circuit voltage-output curve are related to amplitude and frequency, respectively. In addition, the subsequent simulation results, obtained by ANSYS and COMSOL software, were highly consistent with the experimental results. Furthermore, we built a platform to simulate the scene of the car passing through speed bumps, and the difference in height and the number of speed bumps were significantly distinguished according to the output voltage signals. Therefore, the S-TENG has broad application prospects in road transportation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym14132695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9269096PMC
June 2022

First Report of Athelia rolfsii Causing Stem Rot on Sunflower (Helianthus annuus L.) in Sichuan, China.

Plant Dis 2022 Jul 8. Epub 2022 Jul 8.

China West Normal University, 56714, College of Life Science, Nanchong, Sichuan, China, Nanchong, Sichuan, China, 637002;

Sunflower (Helianthus annuus L.) is a major oilseed crop in the world, but recently it has become a popular ornamental flower in China (Kaya ET AL., 2012). Notably, stem rot was frequently observed on 30-day-old sunflower plants in Sichuan Province, P. R. China. In May 2021, 207 plants with stem rot and premature death were found among the investigated 1732 plants, with 11.95% incidence rate. Stem rot were mainly observed on the base stem of plants. Infected stems developed brown lesions that became slightly sunken and covered with white mycelial mats. With disease progress, the affected plants eventually wilted at early stage (Fig. 1A). For pathogen isolation, mycelia were harvested directly from diseased tissue and cultured on potato dextrose agar (PDA) at 25±2℃ in incubator without light. Eighteen fungal isolates with similar colony morphology were consistently isolated by purifying from different sampling areas. These isolates would form radial colonies with white aerial mycelia possessing branches and septa, and some could form clamp connection structure on PDA. Their average growth rate was 27.6 ± 0.09 mm per day (Fig. 1B & C). White granular sclerotia were abundantly produced on 6-day-old colonies. The sclerotia with a diameter of 1.18 ± 0.26 mm (n=348) gradually changed from white to light yellow and finally to brown with age (Fig. 1B). A representative isolate NCSF5.20-8 was identified accurately by multi-locus approach, and its genomic DNA was extracted for amplifying and sequencing the fragments of ITS, 18 S and TEF1-α according to previous description (Zhong et al., 2021). The nucleotide BLAST search of these sequences was nearly 100% identical to Athelia rolfsii (the teleomorph of Sclerotium rolfsii Sacc.) with accession number MN258360.1 (499/499 bp, 100%), AY665774.1 (1428/1428 bp, 100%), and JF267794.1 (489/490, 99%), respectively. The obtained sequences were deposited in GenBank with accession number OK635580.1, OM319631.1 and OK665849.1. Phylogenetic analysis based on simultaneously available ITS, 18S and TEF1-α sequences of Athelia genus indicated that NCSF5.20-8 could be clustered into the A. rolfsii clade with a support value of 100% (Fig. 2). Combining the above morphological characteristics and molecular identification, the isolate NCSF5.20-8 was finally confirmed as the identity of A. rolfsii (Mordue 1974; Zhong et al., 2021). In order to fulfil Koch's postulates and prove the pathogenicity of NCSF5.20-8 to sunflower, mycelial plugs were leaned on the base stem of healthy sunflower plants (cv. Guangwu muntain) with 45-day-old, while plants with same growth treated by PDA plug free of mycelium were used as control. All the inoculated plants were kept in a greenhouse at 25±2°C with 14 h photoperiod and 80% relative humidity. The experiments were conducted for triple biological repeats. Two days after inoculation, all the plants inoculated with NCSF5.20-8 had typical symptoms of stem rot, whereas, the control plants remained asymptomatic (Fig. 1D). Using above mentioned protocol, A. rolfsii was re-isolated only from the symptomatic stem of inoculated plants. To our knowledge, stem rot caused by A. rolfsii on sunflower has been reported in Italy and Turkey (Infantino et al., 1997; Cer & Morca, 2020), but it is the first report in China. For this pathogen has a wide host range with serious destructiveness (Punja, 1985), our research is beneficial to develop strategies to mitigate future losses of sunflower in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-04-22-0875-PDNDOI Listing
July 2022

Study on an attenuated rabies virus strain CTN181-3.

Biologicals 2022 Jun 30. Epub 2022 Jun 30.

National Institutes for Food and Drug Control, Beijing, 102629, China. Electronic address:

We have obtained an attenuated rabies virus CTN181-3. In this paper, we make a comprehensive studies on CTN181-3. CTN181-3 showed no pathogenicity by i. c. or o. i. inoculation in 3-week-old mice, lower pathogenic in 2-week-old mice, and no virulence by o. i. inoculation in 8-week-old golden hamsters. CTN181-3 showed high immunogenicity, which produced high level neutralizing antibodies, 100% sero-conversation and >5.0 IU/ml GMT by one dose i. m. or o. i. vaccination in mice and golden hamsters. Cellular immune response by one dose i. m. or o. i. inoculation was detected. Especially in PEP, reduced dose of vaccination resulted in 50% (one dose) and 100% (2 doses) protections in golden hamsters. Molecular basis of the attenuation indicated that eight substitutions compared to its parental virus strain CTN-1, among them the two substitutions at the G276 (Leu→Val) and L1496 (Met→Trp) were the critical attenuated site. The phenotypic and genotypic characteristics of CTN181-3 were highly stable, no reversion was occurred when the virus was multiple passaged in suckling mice brains, guinea pig submandibular glands or BSR/Vero cell cultures. The gene homology compared to the Chinese rabies isolates showed much higher than rabies vaccine strains used in China, suggesting CTN181-3 is a promising and suitable oral rabies vaccine candidate strain.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biologicals.2022.06.004DOI Listing
June 2022

Risk factors for habitual snoring among children aged 2-14 years in Chengdu, Sichuan.

Sleep Breath 2022 Jun 29. Epub 2022 Jun 29.

Department of Clinical Research Management, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

Purpose: Habitual snoring (HS) is a prominent symptom of sleep-disordered breathing; thus, it is also important to consider the associated, multidimensional risk factors for HS in children. This study was aimed to identify risk factors for HS in children.

Methods: A cross-sectional survey was performed in Chengdu. Children aged 2-14 years from four districts were randomly chosen to participate. Questionnaires were voluntarily completed by the children's guardians.

Results: The survey included 926 boys and 622 girls, who were an average of 6.4 ± 3.0 years old. The sample included 463 habitual snorers (30.4%), 683 occasional snorers (44.8%), and 402 nonsnorers (26.4%). HS was found in 51.8% of children under 7 years old and 26.6% of children aged 7 years old or older. The prevalence of pregnancy complications was significantly lower in mothers who bore children with HS (p = .006). Among the HS group, 86.6% had an immediate family member who snores. Breastfeeding duration among the HS group was significantly less than among the occasional snoring and nonsnoring groups. History of symptoms of allergic rhinitis, rhinosinusitis, tonsillitis, and pneumonia/bronchitis in the past 6 months was associated with HS. Likewise, maternal smoking during pregnancy, maternal exposure to secondhand smoke during pregnancy, and child exposure to secondhand smoke were also associated with HS.

Conclusion: The prevalence of HS was higher in children under 7 years old. Having a mother with more education, a family history of snoring, a shorter period of breastfeeding, upper respiratory tract inflammation, and passive smoking are important risk factors for HS. Pregnancy complications may be associated with lower prevalence of snoring in childhood.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11325-022-02670-7DOI Listing
June 2022

Inhibitors Targeting Multiple Janus Kinases From Mediate Inhibition and Apoptosis Against Gastric Cancer Cells the Estrogen Pathway.

Front Chem 2022 6;10:922110. Epub 2022 Jun 6.

CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China.

Janus kinases (JAKs) play a key role in subtly regulating proliferation, apoptosis, and differentiation of cancer cells, and their inhibitors are actively sought as new drug leads. By developing JAKs based affinity ultrafiltration method coupled with LC/Q-TOF-MS in order to discover selective JAKs inhibitors from total quaternary alkaloids (QAs) from , peak 19 (Berberine) and peak 21 (Chelerythrine) were revealed to exhibit notable selectivity on JAK1, JAK2, and JAK3 over Tyk2. In addition, Chelerythrine showed stronger inhibitory activity than the positive control (Cerdulatinib) on gastric cancer cells (AGS), while Berberine, with weaker inhibition. Chelerythrine and Berberine also showed obvious inhibition on human hepatocyte cells (LO2). Furthermore, molecular docking analysis revealed their discrepancies due to different interaction bonds and characteristic residues. Quaternary N was proposed as the functional group to enhance the selectivity of JAK1, and some specific moieties towards Asp1021, Leu855, and Leu828 were suggested to increase the selectivity for JAK1, JAK2, and JAK3, respectively. As the most potential inhibitor of JAKs from QAs, Chelerythrine exhibited distinct suppression of adhesion, migration, invasion, and stimulating apoptosis of AGS cells, which was consistent with the significant down-regulation of estrogen receptors (ER-α36, ER-α66, and ER-β1) and Src expression. In conclusion, an efficient screening approach was developed to identify Berberine and Chelerythrine as potential selective candidates from with significant anti-proliferative activity against gastric carcinoma. As we know, it was the first report to propose an estrogen signal pathway for Chelerythrine in anti-gastric cancer cells (AGS) study. The results supported Chelerythrine inhibitory effects on AGS by not only direct inhibiting JAKs but also down-regulating the estrogen pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2022.922110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207197PMC
June 2022

Co-silencing of ABA receptors (SlRCAR) reveals interactions between ABA and ethylene signaling during tomato fruit ripening.

Hortic Res 2022 5;9:uhac057. Epub 2022 Jun 5.

Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing 401331, China.

The ripening of fleshy fruits is highly dependent on the regulation of endogenous hormones, including ethylene, abscisic acid (ABA) and other phytohormones. However, the regulatory mechanism of ABA signaling and its interaction with ethylene signaling in fruit ripening are still unclear. In this study, multi-gene interference (RNAi) was applied to silence the ABA receptor genes in tomato for screening the specific receptors that mediate ABA signaling during fruit ripening. The results indicated that the ABA receptors, including SlRCAR9, SlRCAR12, SlRCAR11, and SlRCAR13, participate in the regulation of tomato fruit ripening. Comparative analysis showed that and play more important roles in mediating ABA signaling during tomato fruit ripening. Co-silencing of the four genes encoding these receptors could weaken the ethylene biosynthesis and signaling pathway at the early stage of tomato fruit ripening, leading to delayed fruit ripening. Meanwhile, co-silencing enhanced fruit firmness, and altered the shelf-life and susceptibility to of the transgenic fruits. Furthermore, blocking ABA signaling did not affect the ability of ethylene to induce fruit ripening, whereas the block may inhibit the effectiveness of ABA in promoting fruit ripening. These results suggested that ABA signaling may be located upstream of ethylene signaling in regulating fruit ripening. Our findings provide a new insight into the complex regulatory network of phytohormones in regulating fruit ripening in tomato.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/hr/uhac057DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9171117PMC
June 2022

Immuno-PET imaging of PD-L1 expression in patient-derived lung cancer xenografts with [Ga]Ga-NOTA-Nb109.

Quant Imaging Med Surg 2022 Jun;12(6):3300-3313

NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, China.

Background: Accurate evaluation of programmed death-ligand 1 (PD-L1) expression levels in cancer patients may be useful in the identification of potential candidates for anti-programmed death-1/PD-L1 (anti-PD-1/PD-L1) immune checkpoint therapy to improve the response rate of immune checkpoint blockade therapy. This study evaluated the feasibility of the nanobody-based positron emission tomography (PET) tracer [Ga]Ga-NOTA-Nb109 for immuno-PET imaging of PD-L1 in lung cancer patient-derived xenograft (PDX).

Methods: We constructed 2 PDXs of lung adenocarcinoma (ADC) and lung squamous cell carcinoma (SCC) and used them for immuno-PET imaging. A 2-hour dynamic PET scanning was performed on the samples and the biodistribution and metabolism of [Ga]Ga-NOTA-Nb109 were investigated using region of interest (ROI) analysis. The biodistribution of [Ga]Ga-NOTA-Nb109 in the 2 PDXs was investigated by static PET scanning. In addition, tumor PD-L1 expression in the 2 PDXs was evaluated by autoradiography, western blot, and immunohistochemical (IHC) analysis.

Results: Noninvasive PET imaging showed that [Ga]Ga-NOTA-Nb109 can accurately and sensitively assess the PD-L1 expression in non-small cell lung cancer (NSCLC) PDX models. The maximum [Ga]Ga-NOTA-Nb109 uptake by the ADC PDX LU6424 and the SCC PDX LU6437 were 3.13%±0.35% and 2.60%±0.32% injected dose per milliliter of tissue volume (ID/mL), respectively, at 20 min post injection. and biodistribution analysis showed that [Ga]Ga-NOTA-Nb109 was rapidly cleared through renal excretion and an enhanced signal-to-noise ratio (SNR) was achieved. PD-L1 expression analysis showed good agreement with PET imaging results.

Conclusions: This study demonstrated that [Ga]Ga-NOTA-Nb109 could be applied with PET imaging to noninvasively and accurately monitor PD-L1 expression for screening patients who may be responsive to immunotherapy and to guide the development of appropriate treatment strategies for such patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/qims-21-991DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131318PMC
June 2022

Ethanol Induces Secretion of Proinflammatory Extracellular Vesicles That Inhibit Adult Hippocampal Neurogenesis Through G9a/GLP-Epigenetic Signaling.

Front Immunol 2022 13;13:866073. Epub 2022 May 13.

Bowles Center for Alcohol Studies, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC, United States.

Adult hippocampal neurogenesis (AHN) is involved in learning and memory as well as regulation of mood. Binge ethanol reduces AHN, though the mechanism is unknown. Microglia in the neurogenic niche are important regulators of AHN, and ethanol promotes proinflammatory microglia activation. We recently reported that extracellular vesicles (EVs) mediate ethanol-induced inflammatory signaling in microglia. Therefore, we investigated the role of EVs in ethanol-induced loss of adult hippocampal neurogenesis. At rest, microglia promoted neurogenesis through the secretion of pro-neurogenic extracellular vesicles (pn-EVs). Depletion of microglia using colony-stimulating factor 1 receptor (CSFR1) inhibition or using organotypic brain slice cultures (OBSCs) caused a 30% and 56% loss of neurogenesis in the dentate, respectively, as measured by immunohistochemistry for doublecortin (DCX). Likewise, chemogenetic inhibition of microglia using a CD68.hM4di construct caused a 77% loss in OBSC, indicating a pro-neurogenic resting microglial phenotype. EVs from control OBSC were pro-neurogenic (pn-EVs), enhancing neurogenesis when transferred to other naive OBSC and restoring neurogenesis in microglia-depleted cultures. Ethanol inhibited neurogenesis and caused secretion of proinflammatory EVs (EtOH-EVs). EtOH-EVs reduced hippocampal neurogenesis in naïve OBSC by levels similar to ethanol. Neurogenesis involves complex regulation of chromatin structure that could involve EV signaling. Accordingly, EtOH-EVs were found to be enriched with mRNA for the euchromatin histone lysine methyltransferase (Ehm2t/G9a), an enzyme that reduces chromatin accessibility through histone-3 lysine-9 di-methylation (H3K9me2). EtOH-EVs induced G9a and H3K9me2 by 2-fold relative to pn-EVs in naïve OBSCs. Pharmacological inhibition of G9a with either BIX-01294 or UNC0642 prevented loss of neurogenesis caused by both EtOH and EtOH-EVs. Thus, this work finds that proinflammatory EtOH-EVs promote the loss of adult hippocampal neurogenesis through G9a-mediated epigenetic modification of chromatin structure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2022.866073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136051PMC
May 2022

Bioactive pterocarpans from the root of Astragalus membranaceus var. mongholicus.

Phytochemistry 2022 Aug 21;200:113249. Epub 2022 May 21.

Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy / Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research / International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China, Jinan University, Guangzhou, 510632, People's Republic of China; College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China. Electronic address:

Eleven undescribed and three known pterocarpans were isolated and identified from the traditional Chinese medicine "Huang-qi", Astragali Radix (the root of Astragalus membranaceus var. mongholicus (Bunge) P.K.Hsiao). The structures of these pterocarpans were determined using spectroscopic, X-ray crystallographic, quantum chemical calculation, and chemical methods. Pterocarpans, almost exclusively distributed in the family of Leguminosae, are the second largest subgroup of isoflavanoids. However, pterocarpan glycoside number is limited, most of which are glucosides, and only one pterocarpan apioside was isolated from nature. Notably, nine rare apiosyl-containing pterocarpan glycosides were isolated and identified. The hypoglycemic activities of all these compounds were evaluated using α-glucosidase and DPP-IV inhibitory assays respectively, and some isolates displayed the α-glucosidase inhibitory function. The antioxidant activities of all compounds were evaluated using the ORAC and DPPH radical scavenging assays, respectively. All compounds exhibited varying degrees of oxygen radical absorbance capacity, and some compounds displayed DPPH radical scavenging ability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.phytochem.2022.113249DOI Listing
August 2022

HyProCure for Pediatric Flexible Flatfoot: What Affects the Outcome.

Front Pediatr 2022 14;10:857458. Epub 2022 Apr 14.

Foot & Ankle Section, Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth Peoples Hospital, Shanghai, China.

Background: The high success rate, minimal invasion, and safety of subtalar arthroereisis (SA) have made it a primary mode of surgical management for pediatric flexible flatfoot. The HyProCure procedure is a new surgery for SA, However, very few available studies reported the therapeutic effects of the HyProCure procedure, especially in pediatric flexible flatfoot. The main aim of the present study was to investigate the clinical and radiological outcomes of the HyProCure procedure for pediatric flexible flatfoot and analyze the risk factors for therapeutic outcomes and sinus tarsi pain.

Methods: In this retrospective cohort study, 69 pediatric flexible flatfoot patients (107 feet) who underwent the HyProCure procedure were included between July 2015 and September 2020. All patients underwent the HyProCure procedure with or without gastrocnemius recession. The Maryland foot score (MFS), visual analog scale (VAS), radiographic data, and complications were assessed at a minimum 1-year follow-up and statistically analyzed.

Results: The mean follow-up was 35.9 months (range, 13-73 months). At the last follow-up, VAS (0.64 ± 1.16) was significantly lower than the preoperative VAS (4.06 ± 1.43) ( < 0.001); MFS (90.39 ± 12.10) was significantly higher than the preoperative MFS (71.36 ± 10.25) ( < 0.001). The AP talar-second metatarsal angle (T2MT angle) significantly decreased from 17.0 ± 5.4° preoperatively to 11.4 ± 5.2° at the last follow-up ( < 0.001). The lateral talar-first metatarsal angle (Meary's angle) significantly decreased from 13.8 ± 6.4° preoperatively to 6.3 ± 5.0° at the last follow-up ( < 0.001). The calcaneal declination angle (Pitch angle) significantly increased from 13.5 ± 4.9° preoperatively to 14.8 ± 4.4° at the last follow-up ( < 0.001). Logistic regression analysis indicated that patients with a longer distance from the tail end of the implant exceeding the longitudinal talar bisection line had 275.8% greater odds of MFS < 90. Yet, no risk factors were found in connection with sinus tarsi pain.

Conclusions: The HyProCure procedure for pediatric flexible flatfoot achieved satisfactory curative effects with a low complication rate; implant depth was associated with unsatisfactory postoperative outcome.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fped.2022.857458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047858PMC
April 2022

Inhibition of Drp1 ameliorates diabetic retinopathy by regulating mitochondrial homeostasis.

Exp Eye Res 2022 Jul 28;220:109095. Epub 2022 Apr 28.

Center of Clinical Research, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, PR China. Electronic address:

Diabetic retinopathy (DR) is a potentially blinding complication resulting from diabetes mellitus (DM). Retinal vascular endothelial cells (RMECs) dysfunction occupies an important position in the pathogenesis of DR, and mitochondrial disorders play a vital role in RMECs dysfunction. However, the detailed mechanisms underlying DR-induced mitochondrial disorders in RMECs remain elusive. In the present study, we used High glucose (HG)-induced RMECs in vitro and streptozotocin (STZ)-induced Sprague-Dawley rats in vivo to explore the related mechanisms. We found that HG-induced mitochondrial dysfunction via mitochondrial Dynamin-related protein 1(Drp1)-mediated mitochondrial fission. Drp1 inhibitor, Mdivi-1, rescued HG-induced mitochondrial dysfunction. Protein Kinase Cδ (PKCδ) could induce phosphorylation of Drp1, and we found that HG induced phosphorylation of PKCδ. PKCδ inhibitor (Go 6983) or PKCδ siRNA reversed HG-induced phosphorylation of Drp1 and further mitochondrial dysfunction. The above studies indicated that HG increases mitochondrial fission via promoting PKCδ/Drp1 signaling. Drp1 induces excessive mitochondrial fission and produces damaged mitochondrial, and mitophagy plays a key role in clearing damaged mitochondrial. Our study showed that HG suppressed mitophagy via inhibiting LC3B-II formation and p62 degradation. 3-MA (autophagy inhibitor) aggravated HG-induced RMECs damage, while rapamycin (autophagy agonist) rescued the above phenomenon. Further studies were identified that HG inhibited mitophagy by down-regulation of the PINK1/Parkin signaling pathway, and PINK1 siRNA aggravated HG-induced RMECs damage. Further in-depth study, we propose that Drp1 promotion of Hexokinase II (HK-II) separation from mitochondria, thus inhibiting HK-II-PINK1-mediated mitophagy. In vivo, we found that intraretinal microvascular abnormalities (IRMA), including retinal vascular leakage, acellular capillaries, and apoptosis were increased in STZ-induced DR rats, which were reversed by pretreatment with Mdivi-1 or Rapamycin. Altogether, our findings provide new insight into the mechanisms underlying the regulation of mitochondrial homeostasis and provide a potential treatment strategy for Diabetic retinopathy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.exer.2022.109095DOI Listing
July 2022

-Tetrazine-Bridged Photochromic Aromatic Framework Material.

ACS Omega 2022 Apr 23;7(13):11276-11284. Epub 2022 Mar 23.

Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.

Integrating fluorescent chromophores in aromatic frameworks could not only prevent aggregation-induced quenching caused by the π-π stacking interaction between the chromophore components but also confer new fluorescence properties. Herein, we report the fabrication of -tetrazine-bridged aromatic frameworks by the incorporation of the smallest aromatic fluorophore, -tetrazine (), into the skeleton of a tetrahedrally connected lattice of aromatic frameworks. The thin films of coated on silica gel plates were found to exhibit reversible photoswitching fluorescence characteristics under alternate UV and visible-light irradiations with excellent fluorescence stability and high on/off contrast. The repeatable "on/off"fluorescence photoswitchability of the thin films was mechanistically attributed to light-induced reversible transformation between 's neutral and radical states.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.2c00278DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8992253PMC
April 2022

Development and Applications of Hydrogel-Based Triboelectric Nanogenerators: A Mini-Review.

Polymers (Basel) 2022 Apr 2;14(7). Epub 2022 Apr 2.

Shenzhen Weijian Wuyou Technology Co., Ltd., Shenzhen 518102, China.

In recent years, with the appearance of the triboelectric nanogenerator (TENG), there has been a wave of research on small energy harvesting devices and self-powered wearable electronics. Hydrogels-as conductive materials with excellent tensile properties-have been widely focused on by researchers, which encouraged the development of the hydrogel-based TENGs (H-TENGs) that use the hydrogel as an electrode. Due to the great feasibility of adjusting the conductivity and mechanical property as well as the microstructure of the hydrogels, many H-TENGs with excellent performance have emerged, some of which are capable of excellent outputting ability with an output voltage of 992 V, and self-healing performance which can spontaneously heal within 1 min without any external stimuli. Although there are numerous studies on H-TENGs with excellent performance, a comprehensive review paper that systematically correlates hydrogels' properties to TENGs is still absent. Therefore, in this review, we aim to provide a panoramic overview of the working principle as well as the preparation strategies that significantly affect the properties of H-TENGs. We review hydrogel classification categories such as their network composition and their potential applications on sensing and energy harvesting, and in biomedical fields. Moreover, the challenges faced by the H-TENGs are also discussed, and relative future development of the H-TENGs are also provided to address them. The booming growth of H-TENGs not only broadens the applications of hydrogels into new areas, but also provides a novel alternative for the sustainable power sources.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym14071452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9002585PMC
April 2022

Automated method for short-chain aldehydes emission measurement by dynamic solid-phase microextraction on-fiber derivatization GC-MSD coupled with a flow-cell.

J Chromatogr A 2022 May 27;1671:462996. Epub 2022 Mar 27.

R&D Division, Dow Chem (China) Invest Co. Ltd., Shanghai 201203, China. Electronic address:

A novel automated method was developed to test short-chain aldehyde emissions using a chamber and a flow-cell coupled with a dynamic solid-phase microextraction (SPME) on-fiber derivatization technique. Calibration curves of short-chain aldehydes were developed for quantitation of target analytes, including formaldehyde, acetaldehyde, propionaldehyde, and acrolein emitted from the chamber outlet. The linearity range for the aldehydes was 0.8 to 2130.0 µg/m. The limits of quantitation (LOQ) for various aldehydes ranged from ∼0.2 - 1.9 µg/m. By using this method, emission rate curves are measured with an automated system. Compared with a traditional 2,4-dinitrophenyl hydrazine (DNPH)-high performance liquid chromatography (HPLC) method for aldehyde emission measurement, this method provided an automated technique to study the emission of short-chain aldehydes from multiple samples in one experiment. Furthermore, by using dynamic SPME coupled to an on-fiber derivatization technique, the repeatability and sensitivity of the method is comparable and even better than traditional techniques.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chroma.2022.462996DOI Listing
May 2022

Effect of lactobacteria fermentation on structure and physicochemical properties of Chinese yam starch (Dioscorea opposita Thunb.).

Food Chem 2022 Sep 2;387:132873. Epub 2022 Apr 2.

Guangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. Electronic address:

Biotransformation is an effective technique to modify the structure and physicochemical properties of carbohydrates. In this work, Chinese yam (Dioscorea opposita Thunb.) starch was fermented by lactobacteria. The effect of fermentation time (6, 12, 30, 42 and 72 h) on structure and physicochemical properties of Chinese yam starch were investigated. The microstructure was destroyed after lactobacteria fermentation for 42 and 72 h. The X-ray diffraction pattern of Chinese yam starch indicated a transformed A to A + V crystalline type. → 4)-α-d-glucose-(1 → from backbone and unreduced terminal α-d-glucose-(1 → 4 from branch were identified by NMR spectra, and free glucose was only detected in fermented starch at 72 h. With the extension of fermentation time, the crystallinity and thermal parameters increased within 42 h and thereafter decreased. M, M/M, long chains of DP25-36 and DP ≥ 37, peak viscosity, trough viscosity, finally viscosity and setback presented a reverse trend.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodchem.2022.132873DOI Listing
September 2022

[Arthroscopic treatment of ankle instability combined with anteromedial impingement syndrome].

Zhongguo Gu Shang 2022 Mar;35(3):238-42

Foot & Ankle Section, Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China.

Objective: To investigate the surgical skills and clinical curative results of arthroscopic treatment of ankle instability combined with anteromedial impingement syndrome.

Methods: From February 2019 to August 2020, 13 patients with ankle instability combined with anteromedial impingement were retrospectively analyzed. There were 10 males and 3 females with age of (40.0±15.1) years old. The course of disease was(44.1±33.2) months. All patients had history of ankle sprain. MRI showed the injury of anterior talofibular ligament. All patients had anteromedial pain and pressing pain when ankle dorsiflexion. All patients were treated with ankle debridement and Brostr?m-Gould surgery under ankle arthroscopic. Postoperative results were evaluated by VAS(visual analogue scale) and AOFAS-AH(American Orthopaedic Foot and Ankle Society Ankle-Hindfoot scale, AOFAS-AH).

Results: All 13 patients completed the surgery successfully with an operative time of 60 to 90 minutes. All the surgical incisions healed by first intention, and no complications such as incision infection, skin necrosis and neurovascular injury. Follow-up time was (18.1±4.7) months. At the latest follow-up, the VAS score was 1.2±1.1, which was significantly lower than the preoperative score 4.8±1.5 (<0.05);the AOFAS-AH score 94.2±5.1 was significantly higher than the preoperative score 65.5±11.5 (<0.05). The AOFAS-AH score at the final follow-up ranged from 84 to 100. All patients walked with normal gait without ankle instability or impingement recurrence.

Conclusion: Ankle anteromedial impingement syndrome combined with ankle instability is easy to be ignored clinically. Such kind of anteromedial impingement syndrome is mostly related to osteophyte at dorsal medial talar neck. Arthroscopic treatment of ankle instability combined with anteromedial impingement syndrome has satisfactory curative effect with safety and minimal injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12200/j.issn.1003-0034.2022.03.008DOI Listing
March 2022

Orthogonality catastrophe and quantum speed limit for spin chain at finite temperature.

Sci Rep 2022 Mar 23;12(1):5058. Epub 2022 Mar 23.

Ikerbasque, Basque Foundation for Science, 48011, Bilbao, Spain.

We present an interesting relationship between the orthogonality catastrophe (OC) and the quantum speed limit (QSL) for a spin chain with uniform nearest neighbour couplings perturbed by an impurity spin. We thoroughly study the catastrophic QSL that specifies a bound on the evolution time between the initial and final states and in this respect, link it to the emerging OC effect. It is found that the speed of state evolution subtle but fundamental, and the bound characterized by QSL shows the same behaviours as the OC effect in the thermodynamic limit. It allows us to reveal some universal properties, in particular finite temperature effects. Significantly, the threshold of temperature and system size is clearly demonstrated for the QSL under finite temperature.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-022-09010-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8943030PMC
March 2022

Correction to: Efficacy assessment for low‑level laser therapy in the treatment of androgenetic alopecia: a real‑world study on 1383 patients.

Lasers Med Sci 2022 Aug;37(6):2595

Department of Plastic and Aesthetic Surgery, Nanfang Hospital of Southern Medical University, 1838 North Guangzhou AV, Guangzhou, 510515, Guangdong, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10103-022-03526-yDOI Listing
August 2022

The key to immunotherapy: how to choose better therapeutic biomarkers for patients with non-small cell lung cancer.

Biomark Res 2022 Mar 7;10(1). Epub 2022 Mar 7.

Department of Oncology, The Second Xiangya Hospital, Central South University, Changsha, 410011, Hunan, China.

Immunotherapy has become the standard of care for non-small cell lung cancer (NSCLC), either in combination or monotherapy. However, there are still some patients who cannot benefit from it. Immunization strategies for NSCLC are based on the expression of PD-L1 on tumor cells and TMB, and although these indicators have a certain predictive effect, their predictive performance is not good. Therefore, clinicians must make adjustments to recognize markers. This is a review article that summarized immunotherapeutic biomarkers according to the "seed-soil-environment", generalizes primary resistance to immunotherapy, and summarizes the integration of markers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40364-022-00355-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8900392PMC
March 2022

PRECISION.seq: An R Package for Benchmarking Depth Normalization in microRNA Sequencing.

Front Genet 2021 28;12:823431. Epub 2022 Jan 28.

Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, NY, United States.

We present a new R package for assessing the performance of depth normalization in microRNA sequencing data. It provides a pair of microRNA sequencing data sets for the same set of tumor samples, additional pairs of data sets simulated by re-sampling under various patterns of differential expression, and a collection of numerical and graphical tools for assessing the performance of normalization methods. Users can easily assess their chosen normalization method and compare its performance to nine methods already included in the package. enables an objective and systematic evaluation of normalization methods in microRNA sequencing using realistically distributed and robustly benchmarked data under a wide range of differential expression patterns. To our best knowledge, this is the first such tool available. The data sets and source code of the R package can be found at https://github.com/LXQin/PRECISION.seq.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.823431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8832140PMC
January 2022

Efficacy assessment for low-level laser therapy in the treatment of androgenetic alopecia: a real-world study on 1383 patients.

Lasers Med Sci 2022 Aug 8;37(6):2589-2594. Epub 2022 Feb 8.

Department of Plastic and Aesthetic Surgery, Nanfang Hospital of Southern Medical University, 1838 North Guangzhou AV, Guangzhou, 510515, Guangdong, China.

Low-level laser therapy (LLLT) has been a treatment modality by many androgenetic alopecia (AGA) patients in recent years. It remained unclear as to how long the treatment regime should be maintained, and which characteristics of patients should this be recommended. A real-world study was carried out with an FDA-cleared low-level laser helmet for 1383 patients. Ordinal logistic regression analysis with propensity score matching (PSM) was used to investigate the factors related to efficacy assessment. More than 80% of users were between 18 and 40 years old. The median use times were 133 for mild AGA patients and 142 for moderate-to-severe AGA patients, which equated to 38 weeks and 40 weeks, respectively. The overall clinical effectiveness was nearly 80%. PSM analysis revealed that gender (P = 0.002), use period (P = 0.068), scalp conditions with dandruff, rash, and itchy symptoms were associated with the grading of efficacy assessment. Male users (ordinal OR: 1.35, CI: (1.01, 1.79)); use for more than 180 times or use period for 1 year (ordinal OR: 1.40, CI: (1.11, 1.96)); and those with scalp dandruff (ordinal OR: 1.34, CI: (1.01, 1.87)), rash (ordinal OR: 1.47, CI: (1.04, 2.07)), and itchy symptoms (ordinal OR: 1.51, CI: (1.12, 2.03)) had better efficacy assessments. The recommended treatment regime with low-level laser helmet was more than 1 year or 180 use times. Male patients with dandruff, rash, and itchy symptoms in scalps tended to have a better efficacy assessment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10103-022-03520-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343305PMC
August 2022

HaCYC2c regulating the heteromorphous development and functional differentiation of florets by recognizing HaNDUA2 in sunflower.

Plant Cell Rep 2022 Apr 31;41(4):1025-1041. Epub 2022 Jan 31.

Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University, Nanchong, 637009, Sichuan, China.

Key Message: The overexpression of HaCYC2c and its regulation on HaNDUA2 through transcriptional recognition are important for regulating the heteromorphous development and functional differentiation of ray and disc florets in sunflower. Flower symmetry is closely related to pollinator recruitment and individual fecundity for higher plants and is the main feature used to identify flower type in angiosperms. In sunflower, HaCYC2c regulates floral organ development and floral symmetry, but the specific detail remains unclear. In this study, sunflower long petal mutant (lpm) with HaCYC2c insertion mutation was used to investigate the regulating role of HaCYC2c in the morphogenesis of florets and the transformation of floral symmetry through phenotype, transcriptome, qRT-PCR, and possible protein-gene interactions analyses. Results showed that HaCYC2c was overexpressed after an insertion into the promoter region. This gene could recognize the cis-acting element GGTCCC in the promoter region of HaNDUA2 that might regulate HaNDUA2 and affect other related genes. As a consequence, the abnormal elongation of disc petals and the degradation of male reproductive system occurred at the early development of floral organ in sunflower. Furthermore, this insertion mutation resulted in floral symmetry transformation, from actinomorphy to zygomorphy, thereby making the tubular disc florets transformed into ray-like disc florets in sunflower lpm. The findings suggested that the overexpression of HaCYC2c and its control of HaNDUA2 through transcriptional recognition might be an important regulating node of the heteromorphous development and functional differentiation for ray and disc florets in sunflower. This node contributes to the understanding of the balance between pollinator recruitment capacity of ray florets and fertility of disc florets for the optimization of reproductive efficiency and enhancement of species competitiveness in sunflower.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00299-022-02835-4DOI Listing
April 2022

TGR5 Activation Ameliorates Mitochondrial Homeostasis Regulating the PKCδ/Drp1-HK2 Signaling in Diabetic Retinopathy.

Front Cell Dev Biol 2021 14;9:759421. Epub 2022 Jan 14.

Center of Clinical Research, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, China.

Diabetic retinopathy (DR) is one of the most important microvascular diseases of diabetes. Our previous research demonstrated that bile acid G-protein-coupled membrane receptor (TGR5), a novel cell membrane receptor of bile acid, ameliorates the vascular endothelial cell dysfunction in DR. However, the precise mechanism leading to this alteration remains unknown. Thus, the mechanism of TGR5 in the progress of DR should be urgently explored. In this study, we established high glucose (HG)-induced human retinal vascular endothelial cells (RMECs) and streptozotocin-induced DR rat and . The expression of TGR5 was interfered through the specific agonist or siRNA to study the effect of TGR5 on the function of endothelial cell . Western blot, immunofluorescence and fluorescent probes were used to explore how TGR5 regulated mitochondrial homeostasis and related molecular mechanism. The adeno-associated virus serotype 8-shTGR5 (AAV8-shTGR5) was performed to evaluate retinal dysfunction and further confirm the role of TGR5 in DR by HE staining, TUNEL staining, PAS staining and Evans Blue dye. We found that TGR5 activation alleviated HG-induced endothelial cell apoptosis by improving mitochondrial homeostasis. Additionally, TGR5 signaling reduced mitochondrial fission by suppressing the Ca-PKCδ/Drp1 signaling and enhanced mitophagy through the upregulation of the PINK1/Parkin signaling pathway. Furthermore, our result indicated that Drp1 inhibited mitophagy by facilitating the hexokinase (HK) 2 separation from the mitochondria and HK2-PINK1/Parkin signaling. , intraretinal microvascular abnormalities, including retinal vascular leakage, acellular capillaries and apoptosis, were poor in AAV8-shTGR5-treated group under DR, but this effect was reversed by pretreatment with the mitochondrial fission inhibitor Mdivi-1 or autophagy agonist Rapamycin. Overall, our findings indicated that TGR5 inhibited mitochondrial fission and enhanced mitophagy in RMECs by regulating the PKCδ/Drp1-HK2 signaling pathway. These results revealed the molecular mechanisms underlying the protective effects of TGR5 and suggested that activation of TGR5 might be a potential therapeutic strategy for DR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.759421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8795816PMC
January 2022

High-resolution melting PCR analysis for genotyping the gene polymorphism of TNF-α, TGF-β1, IL-10, and IFN-γ in lung transplant recipients.

Adv Clin Exp Med 2022 May;31(5):547-556

Department of Dermatology, Wuxi No. 2 People's Hospital of Nanjing Medical University, China.

Background: High-resolution melting (HRM) analysis is a genotyping method which has the advantages of simple, rapid, low-cost and closed-tube operation.

Objectives: This study evaluated HRM analysis as an option for detecting the single nucleotide polymorphism (SNP) of cytokine, and profiled the distribution of cytokine gene polymorphism in the lung transplant recipients (LTRs).

Material And Methods: High-resolution melting-polymerase chain reaction (HRM-PCR) assays for genotyping tumor necrosis factor alpha (TNF-α) (-308 A/G), tumor growth factor beta 1 (TGF-β1) (+869 T/C), interleukin 10 (IL-10) (-592 C/A, -819 T/C, -1082 G/A), and interferon gamma (IFN-γ) (+874 T/A) SNPs were developed on the LightCycler® 480. The SNPs of the aforementioned cytokine genes in 322 LTRs and 266 normal controls were detected using HRM-PCR approach. To confirm the accuracy of the HRM-PCR assay, we randomly selected 100 samples from the LTRs and detected the aforementioned SNPs with sequence-specific primer-polymerase chain reaction (SSP-PCR) method, using a commercial kit.

Results: The data show that the HRM-PCR assay can distinguish all the cytokine SNPs, and the results of HRM-PCR analysis are in complete concordance to the genotyping results obtained using a commercial kit (κ = 1.0). Our data also show that the allele and genotype frequencies of the abovementioned cytokine are not significantly different between the LTRs and the control groups (p > 0.05). In addition, we found the genotypes of TGF-β1 +869 associated with high expression phenotype were prevalent in the LTRs. On the contrary, for TNF-α -308, IL-10 and IFN-γ, the genotypes associated with low expression phenotype were most common in the LTRs.

Conclusions: In this study, we described a rapid, low-cost and high-throughput HRM-PCR technology for genotyping cytokine SNPs. Our data may be utilized for future studies examining the associations of cytokine gene polymorphisms with the prognosis of the LTRs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.17219/acem/116757DOI Listing
May 2022

Red yeast rice ameliorates non-alcoholic fatty liver disease through inhibiting lipid synthesis and NF-κB/NLRP3 inflammasome-mediated hepatic inflammation in mice.

Chin Med 2022 Jan 25;17(1):17. Epub 2022 Jan 25.

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, Taipa, China.

Background: Red yeast rice (RYR), a nutraceutical with a profound cholesterol-lowering effect, was found to attenuate non-alcoholic fatty liver disease (NAFLD) in mice. Despite monacolin K in RYR being a specific inhibitor of hydroxymethylglutaryl-coenzyme A reductase (HMCGR), the mechanisms underlying the protective effects of RYR against NAFLD are not fully elucidated.

Methods: Using a mouse model of high-fat diet (HFD) feeding and a cellular model of HepG2 cells challenged by lipopolysaccharide (LPS) and palmitic acid (PA), the possible molecular mechanisms were exploited in the aspects of NF-κB/NLRP3 inflammasome and mTORC1-SREBPs signaling pathways by examining the relevant gene/protein expressions. Subsequently, the correlation between these two signals was also verified using cellular experiments.

Results: RYR ameliorated lipid accumulation and hepatic inflammation in vivo and in vitro. RYR improved lipid metabolism through modulating mTORC1-SREBPs and their target genes related to triglyceride and cholesterol synthesis. Furthermore, RYR suppressed hepatic inflammation by inhibiting the NF-κB/NLRP3 inflammasome signaling. Interestingly, the treatment with RYR or MCC950, a specific NLRP3 inhibitor, resulted in the reduced lipid accumulation in HepG2 cells challenged by LPS plus PA, suggesting that the inhibitory effects of RYR on NLRP3 inflammasome-mediated hepatic inflammation may partially, in turn, contribute to the lipid-lowering effect of RYR.

Conclusions: The modulation of NF-κB/NLRP3 inflammasome and lipid synthesis may contribute to the ameliorative effects of RYR against HFD-induced NAFLD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13020-022-00573-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788078PMC
January 2022

Reply to "Limitations of multivariate survival analysis".

Mod Pathol 2022 07 28;35(7):994-995. Epub 2021 Dec 28.

Department of Pathology, Boston Children's Hospital, Boston, MA, USA.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41379-021-00988-3DOI Listing
July 2022
-->