Publications by authors named "Jian Zhuang"

241 Publications

Long-term outcomes of a totally thoracoscopic approach for reoperative mitral valve replacement: a propensity score matched analysis.

Ann Transl Med 2021 Jun;9(12):987

Department of Cardiovascular Surgery, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Guangzhou, China.

Background: This study aimed to summarize the perioperative and long-term outcomes of patients with previous mitral valve surgery (MVS) undergoing reoperative mitral valve replacement (MVR).

Methods: Data for all reoperative mitral valve replacements (re-MVRs) with or without concomitant tricuspid surgery were analyzed from Guangdong Provincial People's Hospital between January 2013 and December 2019. Propensity score matching resulted in 30 matched pairs with improved balance after matching in baseline covariates. Perioperative data and long-term clinical outcomes were analyzed.

Results: Results are based on the matched cohorts between the two groups. The in-hospital mortality was 3.3% (two deaths) in the entire cohort and was not significantly different between the median sternotomy (MS) group and the totally thoracoscopic (TT) group. Most patients in the TT group had their tracheal intubation removed within 24 hours of surgery. The TT group had a diminished requirement for blood transfusion and a reduced 4-day postoperative chest tube drainage amount. The incidence of early major complications, including all-cause death and reoperation due to bleeding, was lower in the TT group. No significant differences were observed in the 7-year survival probability between the two groups.

Conclusions: The encouraging results regarding the perioperative and long-term outcomes of patients who underwent a TT re-MVR show that this approach is particularly beneficial for patients requiring reoperation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-21-2407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267274PMC
June 2021

Comment on "efficacy of platelet-rich plasma injections in patients with adhesive capsulitis of the shoulder".

Int Orthop 2021 Jul 8. Epub 2021 Jul 8.

Department of Bone Tumor, Qilu Hospital of Shandong University, Qingdao, 266035, Shandong, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00264-021-05065-7DOI Listing
July 2021

Predicting reintervention after thoracic endovascular aortic repair of Stanford type B aortic dissection using machine learning.

Eur Radiol 2021 Jun 22. Epub 2021 Jun 22.

Department of Catheterization Lab, Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, No. 106 Zhongshan Er Road, Guangzhou, 510080, People's Republic of China.

Objectives: To construct models for predicting reintervention after thoracic endovascular aortic repair (TEVAR) of Stanford type B aortic dissection (TBAD).

Methods: A total of 192 TBAD patients who underwent TEVAR were included; 68 (35.4%) had indications for reintervention. Clinical characteristics, aorta characteristics on pre- and postoperative computed tomography angiography, and aorta characteristics on immediate postoperative aortic digital subtraction angiography were collected. The least absolute shrinkage and selection operator (LASSO) regression was applied to identify the risk factors for reintervention. Eight classifiers were used for modeling. The models were trained on 100 train-validation random splits with a ratio of 2:1. The performance was evaluated by the receiver operating characteristic curve.

Results: Seven predictors of reintervention were identified, including maximum false lumen diameter, aortic diameter measured at the level of approximately 15 mm distal to the left subclavian artery, aortic diameter measured at the level of the diaphragm, false lumen diameter measured at the level of the celiac artery, number of bare-metal and covered stents, number of bare-metal stents, and residual perfusion of the false lumen. Logistic regression (LR) yielded the highest performance, with an area under the curve of 0.802. A nomogram built for clinical use showed good calibration. The cutoff value for dividing patients into low- and high-risk subgroups was 0.413. Kaplan-Meier curves showed that the overall survival of high-risk patients was significantly shorter than that of low-risk patients (both p < 0.05).

Conclusion: Our nomogram could predict the reintervention after TEVAR in patients with TBAD, which may facilitate patient selection and surveillance strategies.

Key Points: • Seven risk factors of reintervention after TEVAR of TBAD were identified for modeling. • Logistic regression performed best in predicting reintervention with an AUC of 0.802. • Patients with a high risk of reintervention had shorter OS than those with a low risk.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00330-021-07849-2DOI Listing
June 2021

Automatic Facial Recognition of Williams-Beuren Syndrome Based on Deep Convolutional Neural Networks.

Front Pediatr 2021 19;9:648255. Epub 2021 May 19.

Department of Pediatric Cardiology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangdong Cardiovascular Institute, Guangzhou, China.

Williams-Beuren syndrome (WBS) is a rare genetic syndrome with a characteristic "elfin" facial gestalt. The "elfin" facial characteristics include a broad forehead, periorbital puffiness, flat nasal bridge, short upturned nose, wide mouth, thick lips, and pointed chin. Recently, deep convolutional neural networks (CNNs) have been successfully applied to facial recognition for diagnosing genetic syndromes. However, there is little research on WBS facial recognition using deep CNNs. The purpose of this study was to construct an automatic facial recognition model for WBS diagnosis based on deep CNNs. The study enrolled 104 WBS children, 91 cases with other genetic syndromes, and 145 healthy children. The photo dataset used only one frontal facial photo from each participant. Five face recognition frameworks for WBS were constructed by adopting the VGG-16, VGG-19, ResNet-18, ResNet-34, and MobileNet-V2 architectures, respectively. ImageNet transfer learning was used to avoid over-fitting. The classification performance of the facial recognition models was assessed by five-fold cross validation, and comparison with human experts was performed. The five face recognition frameworks for WBS were constructed. The VGG-19 model achieved the best performance. The accuracy, precision, recall, F1 score, and area under curve (AUC) of the VGG-19 model were 92.7 ± 1.3%, 94.0 ± 5.6%, 81.7 ± 3.6%, 87.2 ± 2.0%, and 89.6 ± 1.3%, respectively. The highest accuracy, precision, recall, F1 score, and AUC of human experts were 82.1, 65.9, 85.6, 74.5, and 83.0%, respectively. The AUCs of each human expert were inferior to the AUCs of the VGG-16 (88.6 ± 3.5%), VGG-19 (89.6 ± 1.3%), ResNet-18 (83.6 ± 8.2%), and ResNet-34 (86.3 ± 4.9%) models. This study highlighted the possibility of using deep CNNs for diagnosing WBS in clinical practice. The facial recognition framework based on VGG-19 could play a prominent role in WBS diagnosis. Transfer learning technology can help to construct facial recognition models of genetic syndromes with small-scale datasets.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fped.2021.648255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170407PMC
May 2021

miR-590-5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines.

Oncol Lett 2021 Jul 17;22(1):532. Epub 2021 May 17.

The Center for Heart Development, State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, P.R. China.

Required for meiotic nuclear division 5 homolog A (RMND5A) functions as an E3 ubiquitin ligase. To date, few studies have investigated the role of RMND5A in cancer. In the present study, the expression levels of RMND5A in multiple types of cancer were analyzed using the Gene Expression Profiling Interactive Analysis platform. The results revealed that RMND5A was highly expressed and associated with overall survival in patients with pancreatic adenocarcinoma (PAAD). A wound-healing assay revealed that RMND5A overexpression significantly increased cell migration in the PAAD cell lines AsPC-1 and PANC-1. analysis predicted that RMND5A was a potential target of microRNA(miR)-590-5p. Further experiments demonstrated that overexpression of miR-590-5p downregulated the expression levels of RMND5A and decreased the migratory ability of the AsPC-1 and PANC-1 cell lines. In addition, overexpression of miR-590-5p attenuated the promoting effects of RMND5A on the migration of AsPC-1 and PANC-1 cells. The results of the present study may further elucidate the mechanisms underlying PAAD progression and provide novel targets for the treatment of PAAD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/ol.2021.12793DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156640PMC
July 2021

The cardioprotective effect and mechanism of bioactive glass on myocardial reperfusion injury.

Biomed Mater 2021 Jun 22;16(4). Epub 2021 Jun 22.

National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, People's Republic of China.

Myocardial reperfusion treatment for ischemic infarction may cause lethal injury of cardiomyocytes, which is known as ischemia/reperfusion (I/R) injury. As a kind of prospective biomaterial with superior properties, the application of bioactive glasses (BGs) in myocardial tissue engineering have received great interests. In this study, the cardioprotective effect and relevant mechanism of BG on myocardial reperfusion injury were investigated. H9c2 cardiomyocytes were pretreated with BG extracts and then cultured in hypoxic environment for 30 min followed by reoxygenation for 1 h. The activity of released lactate dehydrogenase (LDH) and the content of malondialdehyde (MDA) in H9c2 cells were tested by assay kits. Cell viability was analyzed by Live/Dead staining assay and the number of living cells was detected by Cell Counting Kit-8 (CCK-8) assay. The cytoskeletal protein F-actin was stained and observed under inverted fluorescence microscope. Mitochondrial membrane potential (MMP) level, reactive oxygen species (ROS) production and apoptosis ratio were evaluated by fluorescent observation and flow cytometry simultaneously. The gene expressions relevant to apoptosis were detected by quantitative real time polymerase chain reaction (qRT-PCR) analysis. The results showed that BG extracts effectively inhibited hypoxia/reoxygenation (H/R)-induced cell injury by suppressing oxidative stress and mitochondrial permeability transition (MPT) within H9c2 cells. Meanwhile, apoptosis caused by H/R injury was alleviated and three classic apoptotic signaling pathways were proved to be regulated by BG extracts. Further analysis showed that phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway was up-regulated in H/R-induced H9c2 cells by BG extracts, leading to relieved cellular apoptosis. These results indicated that BG might exert cardioprotective effect in reperfusion injury when applied in myocardial tissue regeneration and repair.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1748-605X/ac067eDOI Listing
June 2021

Water/gas separation based on the selective bubble-passage effect of underwater superaerophobic and superaerophilic meshes processed by a femtosecond laser.

Nanoscale 2021 Jun;13(23):10414-10424

State Key Laboratory for Manufacturing System Engineering and Shaanxi Key Laboratory of Photonics Technology for Information, School of Electronic Science and Engineering, Xi'an Jiaotong University, Xi'an, 710049, PR China.

To solve the problems caused by tiny bubbles in liquids and the difficulties involved in collecting useful gas underwater, this paper proposes a method to separate bubbles from water by integrating underwater superaerophobic and superaerophilic porous membranes, including bubble removal and collection methods. Inspired by fish scales and lotus leaves, underwater superaerophobic microstructures and underwater superaerophilic microstructures are prepared on a stainless steel (SS) mesh by femtosecond laser processing, respectively. The as-prepared underwater superaerophobic mesh has an anti-bubble ability, while the underwater superaerophilic mesh has a bubble-absorption ability in water. Based on the different dynamic behavior of bubbles on these two kinds of superwetting meshes, efficient water/bubble separation is achieved by using laser-induced superwetting meshes. Tiny bubbles can be completely removed from the water flow in a pipe or easily collected. Such water/gas separation methods based on underwater superaerophobic and superaerophilic porous membranes provide an effective way to prevent the damage caused by bubbles and to collect the available gas in liquids, which has great potential applications in energy utilization, environmental protection, medical and health care, microfluidic chips, chemical manufacturing, agricultural breeding, and so on.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1nr01225hDOI Listing
June 2021

Ordered Fibril Arrays in Osteons Promote the Multidirectional Nanodeflection of Cracks: AFM Imaging.

ACS Biomater Sci Eng 2021 06 20;7(6):2372-2382. Epub 2021 May 20.

School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, Guangdong, P. R. China.

The high fracture resistance of cortical bone is not completely understood across its complex hierarchical structure, especially on micro- and nanolevels. Here, a novel bending test combined with atomic force microscopy (AFM) is utilized to assess the micro-/nanoscale failure behavior of cortical bone under the external load. Unlike the smoother crack path in the transverse direction, the multilevel composite material model endows the longitudinal direction to show multilevel Y-shaped cracks with more failure interfaces for enhancing the fracture resistance. In the lamellae, the nanocracks originating from the interfibrillar nanointerface deflect multidirectionally at certain angles related to the periodic ordered arrangement of the mineralized collagen fibril (MCF) arrays. The ordered MCF arrays in the lamellae may use the nanodeflection of the dendritic nanocracks to adjust the direction of the crack tip, which subsequently reaches the interlamellae to sharply deflect and finally form a zigzag path. This work provides an insight into the relationship between the structure and the function of bone at a multilevel under load, specifically the role of the ordered MCF arrays in the lamellar structure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsbiomaterials.0c01671DOI Listing
June 2021

The Hemodynamics of Patent Ductus Arteriosus in Patients after Central Shunt Operation.

Comput Math Methods Med 2021 24;2021:6675613. Epub 2021 Apr 24.

Department of Catheterization Lab, Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

A central shunt (CS) was an important surgery of systemic-to-pulmonary shunt (SPS) for the treatment of complex congenital heart diseases with decreased pulmonary blood flow (CCHDs-DPBF). There was no clear conclusion on how to deal with unclosed patent ductus arteriosus (PDA) during CS surgery. This study expanded the knowledge base on PDA by exploring the influence of the closing process of the PDA on the hemodynamic parameters for the CS model. The initial three-dimensional (3D) geometry was reconstructed based on the patient's computed tomography (CT) data. Then, a CS configuration with three typical pulmonary artery (PA) dysplasia structures and different sizes of PDA was established. The three-element windkessel (3WK) multiscale coupling model was used to define boundary conditions for transient simulation through computational fluid dynamics (CFD). The results showed that the larger size of PDA led to a greater systemic-to-pulmonary shunt ratio ( ), and the flow ratio of the left pulmonary artery (LPA) to right pulmonary artery (RPA) ( ) was more close to 1, while both the proportion of high wall shear stress (WSS) areas and power loss decreased. The case of PDA nonclosure demonstrates that the aortic oxygen saturation (Sao) increased, while the systemic oxygen delivery (Do) decreased. In general, for the CS model with three typical PA dysplasia, the changing trends of hemodynamic parameters during the spontaneous closing process of PDA were roughly identical, and nonclosure of PDA had a series of hemodynamic advantages, but a larger PDA may cause excessive PA perfusion and was not conducive to reducing cyanosis symptoms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6675613DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093051PMC
April 2021

Bioactive glass activates VEGF paracrine signaling of cardiomyocytes to promote cardiac angiogenesis.

Mater Sci Eng C Mater Biol Appl 2021 May 26;124:112077. Epub 2021 Mar 26.

National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, PR China; School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, PR China; Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou 510006, PR China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, PR China. Electronic address:

The heart contains a wide range of cell types, which are not isolated but interact with one another via multifarious paracrine, autocrine and endocrine factors. In terms of cardiac angiogenesis, previous studies have proved that regulating the communication between cardiomyocytes and endothelial cells is efficacious to promote capillary formation. Firstly, this study investigated the effect and underlying mechanism of bioactive glass (BG) acted on vascular endothelial growth factor (VEGF) paracrine signaling in cardiomyocytes. We found that bioactive ions released from BG significantly promoted the VEGF production and secretion of cardiomyocytes. Subsequently, we proved that cardiomyocyte-derived VEGF played an important role in mediating the behavior of endothelial cells. Further research showed that the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/hypoxia-inducible factor 1α (HIF-1α) signaling pathway was upregulated by BG, which was involved in VEGF expression of cardiomyocytes. This study revealed that by means of modulating cellular crosstalk via paracrine signaling of host cells in heart is a new direction for the application of BGs in cardiac angiogenesis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.msec.2021.112077DOI Listing
May 2021

Nomogram to predict survival outcome of patients with veno-arterial extracorporeal membrane oxygenation after refractory cardiogenic shock.

Postgrad Med 2021 May 20:1-10. Epub 2021 May 20.

Department of Critical Care Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, The Second School of Clinical Medicine, Southern Medical University, Gaozhou, Guangdong, China.

: This study aims to develop a nomogram model to predict the survival of refractory cardiogenic shock (RCS) patients that received veno-arterial extracorporeal membrane oxygenation (VA-ECMO).: A total of 235 and 209 RCS patients were supported with VA-ECMO from January 2018 to December 2019 in Guangdong Provincial People's Hospital, and from January 2020 to December 2020 in four third-grade and class-A hospitals were a development cohort (DC) and validation cohort (VC), respectively. Finally, 137 and 98 patients were included in the DC and VC. Multivariate logistic regression analysis was used to identify variables, and only these independent risk factors were used to establish the nomogram model. The receiver operating characteristic curve (ROC), calibration plot, decision curve, and clinical impact curves were used to evaluate the nomogram's discriminative ability, predictive accuracy, and clinical application value.: Pre-ECMO cardiogenic arrest (pre-ECA), lactate (Lac), inotropic score (IS), and modified nutrition risk in the critically ill score (mNUTRIC score) were incorporated into the nomogram. This showed good discrimination in the DC, with an area under ROC (AUROC) and a 95% confidence interval (CI) of 0.959 (0.911-0.986). The AUROC (95% CI) of the VC was 0.928 (0.858-0.971). The calibration plots of the DC and VC presented good calibration results. The decision curve and clinical impact curve of the nomogram provided improved benefits for RCS patients.: This study established a prediction nomogram composed of pre-ECA, Lac, IS, and mNUTRIC scores that could help clinicians to predict the survival probability at hospital discharge precisely and rapidly for RCS patients that received VA-ECMO.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/00325481.2021.1925562DOI Listing
May 2021

Light-Addressable Square Wave Voltammetry (LASWV) Based on a Field-Effect Structure for Electrochemical Sensing and Imaging.

ACS Sens 2021 04 8;6(4):1636-1642. Epub 2021 Apr 8.

Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China.

Here, we describe a new photoelectrochemical imaging method termed light-addressable square wave voltammetry (LASWV). It measures local SWV currents at an unstructured electrolyte/insulator/semiconductor (EIS) field-effect substrate by illuminating and addressing the substrate with an intensity-constant laser. Due to the continuous generation of charge carriers in the light-irradiated semiconductor, the drift and diffusion of photoinjected carriers within the semiconductor bulk would slow down the equilibrium processes of charge and discharge in one potential pulse cycle. Therefore, even though SWV is sampled at the end of the direct and reverse pulses to reject capacitive currents, in our approach, photoinduced capacitive current can still be detected as an effective sensory signal. The obtained current-potential (-) curve shows a typical shape corresponding to the accumulation, depletion, and inversion regions of field-effect devices. We demonstrated that LASWV can be used as a field-effect chemical sensor to measure the solution pH and monitor enzymatic reactions. More importantly, since the charge carriers are only generated in the illuminated area, the laser spot in the device can be used as a virtual probe to record local electrochemical properties such as impedance with microresolution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acssensors.1c00170DOI Listing
April 2021

A machine learning-based pulmonary venous obstruction prediction model using clinical data and CT image.

Int J Comput Assist Radiol Surg 2021 Apr 31;16(4):609-617. Epub 2021 Mar 31.

Department of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Dongchuan Rd 96, Guangzhou, 510080, China.

Purpose: In this study, we try to consider the most common type of total anomalous pulmonary venous connection and established a machine learning-based prediction model for postoperative pulmonary venous obstruction by using clinical data and CT images jointly.

Method: Patients diagnosed with supracardiac TPAVC from January 1, 2009, to December 31, 2018, in Guangdong Province People's Hospital were enrolled. Logistic regression were applied for clinical data features selection, while a convolutional neural network was used to extract CT images features. The prediction model was established by integrating the above two kinds of features for PVO prediction. And the proposed methods were evaluated using fourfold cross-validation.

Result: Finally, 131 patients were enrolled in our study. Results show that compared with traditional approaches, the machine learning-based joint method using clinical data and CT image achieved the highest average AUC score of 0.943. In addition, the joint method also achieved a higher sensitivity of 0.828 and a higher positive prediction value of 0.864.

Conclusion: Using clinical data and CT images jointly can improve the performance significantly compared with other methods that using only clinical data or CT images. The proposed machine learning-based joint method demonstrates the practicability of fully using multi-modality clinical data.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11548-021-02335-yDOI Listing
April 2021

A three-dimensional printing navigational template combined with mixed reality technique for localizing pulmonary nodules.

Interact Cardiovasc Thorac Surg 2021 04;32(4):552-559

Guangdong Lung Cancer Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Objectives: Localizing non-palpable pulmonary nodules is challenging for thoracic surgeons. Here, we investigated the accuracy of three-dimensional (3D) printing technology combined with mixed reality (MR) for localizing ground glass opacity-dominant pulmonary nodules.

Methods: In this single-arm study, we prospectively enrolled patients with small pulmonary nodules (<2 cm) that required accurate localization. A 3D-printing physical navigational template was designed based on the reconstruction of computed tomography images, and a 3D model was generated through the MR glasses. We set the deviation distance as the primary end point for efficacy evaluation. Clinicopathological and surgical data were obtained for further analysis.

Results: Sixteen patients with 17 non-palpable pulmonary nodules were enrolled in this study. Sixteen nodules were localized successfully (16/17; 94.1%) using this novel approach with a median deviation of 9 mm. The mean time required for localization was 25 ± 5.2 min. For the nodules in the upper/middle and lower lobes, the median deviation was 6 mm (range, 0-12.0) and 16 mm (range, 15.0-20.0), respectively. The deviation difference between the groups was significant (Z = -2.957, P = 0.003). The pathological evaluation of resection margins was negative.

Conclusions: The 3D printing navigational template combined with MR can be a feasible approach for localizing pulmonary nodules.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/icvts/ivaa300DOI Listing
April 2021

Myogenin suppresses apoptosis induced by angiotensin II in human induced pluripotent stem cell-derived cardiomyocytes.

Biochem Biophys Res Commun 2021 05 17;552:84-90. Epub 2021 Mar 17.

Department of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, 510100, China. Electronic address:

Background: Angiotensin II (Ang II), an important component of the renin-angiotensin system (RAS), plays a critical role in the pathogenesis of cardiovascular disorders. In addition, human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been considered as a promising platform for studying personalized medicine for heart diseases. However, whether Ang II can induce the apoptosis of hiPSC-CMs is not known.

Methods: In this study, we treated hiPSC-CMs with different concentrations of Ang II [0 nM (vehicle as a control), 1 nM, 10 nM, 100 nM, 1 μM, 10 μM, 100 μM, and 1 mM] for various time periods (24 h, 48 h, 6 days, and 10 days) and analyzed the viability and apoptosis of hiPSC-CMs.

Results: We found that treatment with 1 mM Ang II for 10 days reduced the viability of hiPSC-CMs by 41% (p = 2.073E-08) and increased apoptosis by 2.74-fold, compared to the control group (p = 6.248E-12). MYOG, which encodes the muscle-specific transcription factor myogenin, was also identified as an apoptosis-suppressor gene in Ang II-treated hiPSC-CMs. Ectopic MYOG expression decreased the apoptosis and increased the viability of Ang II-treated hiPSC-CMs. Further analysis of the RNA sequencing (RNA-seq) data illustrated that myogenin ameliorated Ang II-induced apoptosis of hiPSC-CMs by downregulating the expression of proinflammatory genes.

Conclusion: Our findings suggest that Ang II induces the apoptosis of hiPSC-CMs and that myogenin attenuates Ang II-induced apoptosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrc.2021.03.031DOI Listing
May 2021

Analysis and improvement of positioning reliability and accuracy of theta pipette configuration for scanning ion conductance microscopy.

Ultramicroscopy 2021 May 2;224:113240. Epub 2021 Mar 2.

Key Laboratory of Education Ministry for Modern Design Rotor-Bearing System, Xi'an Jiaotong University, Xi'an 710049, PR China; School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, PR China; Department of Anesthesiology, Honghui Hospital, Xi'an Jiaotong University, Xi'an 710068, PR China.

Scanning ion conductance microscopy (SICM) as an emerging non-contact scanning probe microscopy technique and featuring its strong in-situ detectability for soft and viscous samples, is increasingly used in biomedical and materials related studies. In SICM measurements, employing theta pipette as SICM probe to scan sample is an effective method to extend the applications of SICM for multi-parameter measurement. There are two crucial but still unclear issues that influence the reliability and accuracy of the usage of theta pipette in the SICM measurements, which are the safe feedback threshold and the horizontal measurement offset. In this work, aiming at the theta pipette configuration of SICM, we systematically investigated the two issues of the theta pipette by both finite element method (FEM) simulation and SICM experiments. The FEM analysis results show that the safe feedback threshold of the one side barrel of the theta pipette is above 99.5%, and the horizontal measurement offset is ~0.53 times of the inner radius of the probe tip. Based on this, we proposed an improved scanning method used by the theta pipette to solve the reliability and accuracy problems caused by the feedback threshold too close to the reference current (100%) and the measurement offset error at the tip radius level. Then through testing the polydimethylsiloxane (PDMS) samples with different embossed patterns with the improved method of SICM, we can conclude that the improved method can enhance the scanning reliability by adding the double barrels approaching process and increase the positioning accuracy by compensating an offset distance. The theoretical analysis and the improved scanning method in this work demonstrate more property and usage details of the theta pipette, and further improve the reliability and accuracy of the diversified multifunctional applications of the theta pipette for SICM to meet the increasingly complex and precise research needs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ultramic.2021.113240DOI Listing
May 2021

Fluid-structure interactions (FSI) based study of low-density lipoproteins (LDL) uptake in the left coronary artery.

Sci Rep 2021 Feb 26;11(1):4803. Epub 2021 Feb 26.

Institute of Biomechanics, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.

The purpose of this study is to compare the effect of the different physical factors on low-density lipoproteins (LDL) accumulation from flowing blood to the arterial wall of the left coronary arteries. The three-dimensional (3D) computational model of the left coronary arterial tree is reconstructed from a patient-specific computed tomography angiography (CTA) image. The endothelium of the coronary artery is represented by a shear stress dependent three-pore model. Fluid-structure interaction ([Formula: see text]) based numerical method is used to study the LDL transport from vascular lumen into the arterial wall. The results show that the high elastic property of the arterial wall decreases the complexity of the local flow field in the coronary bifurcation system. The places of high levels of LDL uptake coincide with the regions of low wall shear stress. In addition, hypertension promotes LDL uptake from flowing blood in the arterial wall, while the thickened arterial wall decreases this process. The present computer strategy combining the methods of coronary CTA image 3D reconstruction, [Formula: see text] simulation, and three-pore modeling was illustrated to be effective on the simulation of the distribution and the uptake of LDL. This may have great potential for the early prediction of the local atherosclerosis lesion in the human left coronary artery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-84155-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910311PMC
February 2021

regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.

Am J Physiol Heart Circ Physiol 2021 04 26;320(4):H1634-H1645. Epub 2021 Feb 26.

The Center for Heart Development, State Key Laboratory of Development Biology of Freshwater Fish, Key Laboratory of Protein Chemistry and Developmental Biology of Fish of Ministry of Education, The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development College of Life Sciences, Hunan Normal University, Changsha, China.

Wnt/β-catenin signaling plays a key role in pathological cardiac remodeling in adults. The identification of a tissue-specific Wnt/β-catenin interaction factor may provide a tissue-specific clinical targeting strategy. encodes the core interaction factor of Wnt/β-catenin. Two homologs ( and ) have been identified in mammals. Different from the ubiquitous expression profile of , is enriched in cardiac tissue. However, the role of in mammalian cardiac disease is yet to be elucidated. In this study, we found that was upregulated in human cardiac tissues with pathological hypertrophy. Cardiac-specific overexpression of in mice spontaneously led to cardiac hypertrophy accompanied by declined cardiac function, increased heart weight/body weight and heart weight/tibial length ratios, and increased cell size. The canonical β-catenin/T-cell transcription factor 4 (TCF4) complex was abundant in -overexpressing transgenic (-TG) cardiac tissue, and the downstream genes of Wnt signaling, that is, , , and c-Myc, were upregulated. A tail vein injection of β-catenin inhibitor effectively rescued the phenotype of cardiac failure and pathological myocardial remodeling in -TG mice. Furthermore, in vivo downregulated during cardiac hypertrophic condition antagonized agonist-induced cardiac hypertrophy. Therefore, our study is the first to present in vivo evidence demonstrating that regulates pathological cardiac hypertrophy in a canonical Wnt/β-catenin-dependent manner, which may provide new clues for tissue-specific clinical treatment via targeting this pathway. In this study, we found that is associated with human pathological hypertrophy. Cardiac-specific overexpression of in mice spontaneously led to cardiac hypertrophy. Meanwhile, cardiac function was improved when expression of was interfered in hypertrophy-model mice. Our study is the first to present in vivo evidence demonstrating that regulates pathological cardiac hypertrophy in a canonical Wnt/β-catenin-dependent manner, which may provide new clues for a tissue-specific clinical treatment targeting this pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1152/ajpheart.00538.2020DOI Listing
April 2021

Asporin is a Potential Promising Biomarker for Common Heart Failure.

DNA Cell Biol 2021 Feb;40(2):303-315

Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital and Guangdong Academy of Medical Sciences, School of Medicine, South China University of Technology, Guangzhou, China.

Heart failure (HF) is the end-stage of various diseases, especially ischemic cardiomyopathy (ICM) and dilated cardiomyopathy (DCM). We aimed to investigate the common molecular mechanism of ICM and DCM. Differentially expressed genes (DEGs) of ICM or DCM samples compared with control were identified in GSE1869, GSE5406, GSE57338, GSE79962, GSE116250, and GSE46224 datasets. Functional enrichment analysis and protein-protein network analysis of the coregulated DEGs in at least four datasets were performed using the online tools of DAVID, the Metascape database, and the STRING database. Hub genes of HF were identified and validated by western blotting (WB) and immunohistochemistry in our tissue microarray (TMA). Seventy-four coregulated ICM and 126 coregulated DCM relevant DEGs were identified. Moreover, 59 common genes between ICM and DCM relevant DEGs were obtained, which were mainly involved in cardiac fibrosis and several signal pathways, such as Wnt signal pathway, PI3K-Akt signal pathway, and HIF-1A signal pathway. Among the six hub genes with top degrees, asporin (ASPN) had a relatively higher correlation with LVEF. Finally, TMA and WB results revealed that the ASPN protein was significantly increased in ICM and DCM left ventricular samples. The present study revealed some common molecular mechanisms of HF with different causes. Furthermore, ASPN may be a potential promising biomarker for HF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/dna.2020.5995DOI Listing
February 2021

Quadrangular resection versus chordal replacement for degenerative posterior mitral leaflet prolapse.

Ann Transl Med 2021 Jan;9(1):60

Department of Cardiovascular Surgery, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Guangzhou, China.

Background: The aims of the present study was to compare midterm results of quadrangular leaflet resection versus chordal replacement for the repair of degenerative posterior mitral leaflet (PML) prolapse, and to explore the risk factors for recurrent severe mitral regurgitation (MR).

Methods: From January 2012 to December 2018, 1,423 consecutive patients underwent mitral valve (MV) repair. A total of 317 had degenerative PML prolapse and constituted the study population. Of these, 74 (23.3%) underwent quadrangular leaflet resection, and 243 (76.7%) underwent chordal replacement. Outcomes were compared by using unadjusted data and propensity score-matched analyses.

Results: Patients with multiple leaflet prolapse were more likely to undergo chordal replacement (18.4% 41.9%, P<0.001), and performed as a minimally invasive approach (47.3% 61.7%, P=0.027). Of the entire cohort, 1 death (0.3%) occurred due to intraoperative aortic dissection, and 1 patient who had undergone chordal replacement required reoperation before discharge for posterior leaflet tearing. There was no significant difference in the probability of freedom from recurrent severe MR at 82 months between the resection and neochordae groups in both the pre-matched (95.6% 88.8%, P=0.105) and matched (95.2% 88.5%, P=0.170) cohorts, which was consistent across all of the examined subgroups (P>0.05). Multivariate Cox regression indicated that dilated left ventricular end-systolic diameter (LVESD) was an independent risk factor for recurrent severe MR [<40 >40 mm, hazards ratio (HR): 3.17, 95% confidence interval (CI): 1.20-8.39, P=0.020]; however, surgical technique was not (resection neochordae, HR: 0.31, 95% CI: 0.07-1.37, P=0.122).

Conclusions: Chordal replacement for the repair of degenerative posterior MV prolapse yields similar satisfactory outcomes when compared with quadrangular resection, and is promising in minimally invasive cardiac surgery for various lesions. However, it is also associated with more recurrent severe MR, albeit non-significant, for which patients with dilated LVESD are at high risk.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-20-7475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859790PMC
January 2021

Inflammatory indicator levels in patients undergoing aortic valve replacement via median sternotomy with preoperative anxiety and postoperative complications: a prospective cohort study.

J Int Med Res 2021 Feb;49(2):300060520977417

Department of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Objective: This study was performed to evaluate the association of preoperative anxiety with inflammatory indicators and postoperative complications in patients undergoing scheduled aortic valve replacement surgery.

Methods: A prospective cohort study was performed. The Hamilton Anxiety Scale was used to assess preoperative anxiety. The serum white blood cell (WBC) count and concentrations of C-reactive protein, interleukin (IL)-6, and IL-8 were measured 1 day preoperatively and 3 and 7 days postoperatively. Postoperative complications were also recorded.

Results: Seventy-three patients were included. The incidence of preoperative anxiety was 30.1% (22/73). The payment source was the only independent risk factor for preoperative anxiety. The incidence of postoperative complications was lowest in the mild anxiety group. The WBC count 3 days postoperatively was significantly lower in the mild than moderate-severe anxiety group. The IL-8 concentration 1 day preoperatively was highest in the no anxiety group.

Conclusions: Mild preoperative anxiety might help to improve clinical outcomes. However, further investigations with more patients are warranted. Patients with different degrees of anxiety may have different levels of inflammatory cytokines.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0300060520977417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7869182PMC
February 2021

Micro/nanodevices for assessment and treatment in stomatology and ophthalmology.

Microsyst Nanoeng 2021 29;7:11. Epub 2021 Jan 29.

Department of Engineering Science and Mechanics, Pennsylvania State University, University Park, PA 16802 USA.

Micro/nanodevices have been widely applied for the real-time monitoring of intracellular activities and the delivery of exogenous substances in the past few years. This review focuses on miniaturized micro/nanodevices for assessment and treatment in stomatology and ophthalmology. We first summarize the recent progress in this field by examining the available materials and fabrication techniques, device design principles, mechanisms, and biosafety aspects of micro/nanodevices. Following a discussion of biochemical sensing technology from the cellular level to the tissue level for disease assessment, we then summarize the use of microneedles and other micro/nanodevices in the treatment of oral and ocular diseases and conditions, including oral cancer, eye wrinkles, keratitis, and infections. Along with the identified key challenges, this review concludes with future directions as a small fraction of vast opportunities, calling for joint efforts between clinicians and engineers with diverse backgrounds to help facilitate the rapid development of this burgeoning field in stomatology and ophthalmology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41378-021-00238-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844113PMC
January 2021

Primary sutureless repair for infracardiac total anomalous pulmonary venous connection.

Eur J Cardiothorac Surg 2021 05;59(5):959-966

Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.

Objectives: This study aimed to review surgical experiences in patients with infracardiac total anomalous pulmonary venous connection (TAPVC).

Methods: This retrospective study included 63 infants who underwent conventional repair (n = 27) and sutureless repair (n = 36) from 1 February 2009 to 31 June 2019. Kaplan-Meier curves and Cox regressions were applied to analyse the overall survival and risk factors. Cumulative incidence curves and competing risk models were used to evaluate postoperative pulmonary venous obstruction (PVO).

Results: There were 4 hospital deaths and 2 late deaths, and 8 patients experienced postoperative PVO. The survival rates at 30 days, 1 year and 5 years were 95.2%, 90.5% and 90.5%, respectively. The overall survival rate was significantly higher in the sutureless group than that in the conventional group. The cumulative incidence of postoperative PVO in the conventional group was higher than that in the sutureless group. Univariable Cox regression analyses showed that lower surgical weight, increase in preoperative international normalized ratio, prolonged cardiopulmonary bypass time and aortic cross-clamp time and longer duration of postoperative ventilation were associated with higher mortality. Longer cardiopulmonary bypass time, lower preoperative prothrombin activity and the increasing preoperative international normalized ratio before surgical repair were associated with a higher incidence of postoperative PVO.

Conclusions: Both sutureless and conventional repairs for patients with infracardiac TAPVC achieved favourable postoperative outcomes. There was no death in the sutureless repair group. Compared to conventional repair, sutureless repair was associated with lower mortality and lower incidence of restenosis in pulmonary veins and anastomosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/ejcts/ezaa470DOI Listing
May 2021

FLNC and MYLK2 Gene Mutations in a Chinese Family with Different Phenotypes of Cardiomyopathy.

Int Heart J 2021 Jan 16;62(1):127-134. Epub 2021 Jan 16.

Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences.

Mutations in the sarcomeric protein filamin C (FLNC) gene have been linked to hypertrophic cardiomyopathy (HCM), as they have been determined to increase the risk of ventricular arrhythmia and sudden death. Thus, in this study, we identified a novel missense mutation of FLNC in a Chinese family with HCM, and, interestingly, a second novel truncating mutation of MYLK2 was discobered in one family member with different phenotype.We performed whole-exome sequencing in a Chinese family with HCM of unknown cause. To determine and confirm the function of a novel mutation of FLNC, we introduced the mutant and wild-type gene into AC16 cells (human cardiomyocytes): we then used western blotting to analyze the expression of FLNC in subcellular fractions, and confocal microscope to observe the subcellular distribution of the protein.As per our findings, we were able to identify a novel missense single nucleotide variant (FLNC c.G5935A [p.A1979T]) in the family, which segregates with the disease. FLNC expression levels were observed to be equivalent in both wild-type and p.A1979T cardiomyocytes. However, the expression of the mutant protein has resulted in cytoplasmic protein aggregations, in contrast to wild-type FLNC, which was distributed in the cytoplasm and did not form aggregates. Unexpectedly, a second truncating mutation, NM_033118:exon8:c.G1138T:p.E380X of the MYLK2 gene, was identified in the mother of the proband with dilated cardiomyopathy, which was not found in other subjects.We then identified the FLNC A1979T mutation as a novel pathogenic variant associated with HCM in a Chinese family as well as a second causal mutation in a family member with a distinct phenotype. The possibility that there is more than one causal mutation in cardiomyopathy warrants clinical attention, especially for patients with atypical clinical features.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1536/ihj.20-351DOI Listing
January 2021

Fabrication of Anti-Reflective Surface with Superhydrophobicity/High Oleophobicity and Enhanced Mechanical Durability via Nanosecond Laser Surface Texturing.

Materials (Basel) 2020 Dec 13;13(24). Epub 2020 Dec 13.

College of Vehicle and Transportation Engineering, Henan University of Science and Technology, 48 Xiyuan Road, Jianxi District, Luoyang 471003, China.

In this work, anti-reflective surface with superhydrophobicity/oleophobicity and enhanced abrasion resistance was fabricated on steel alloy surface. Two different surface patterns (i.e., parallel microgrooves and spot arrays) were created by nanosecond laser ablation and chemical immersion. The surface micro/nanostructure, spectral reflectance, wettability, and abrasion resistance of all the samples were determined. The experimental results showed that the laser-chemical treated surfaces exhibited much lower spectral reflectance and significantly enhanced surface integrities compared with the untreated surface. Firstly, the contact angles of water, glycerol, and engine oil on the laser-chemical treated surfaces were increased up to 158.9°, 157.2°, and 130.0° respectively, meaning the laser-chemical treated surfaces achieved both superhydrophobicity and high oleophobicity. Secondly, the laser-chemical treated surface showed enhanced abrasion resistance. The experimental results indicated that the spectral reflectance of the laser-chemical treated surfaces remained almost unchanged, while the laser-chemical treated surface patterned with parallel microgrooves sustained superhydrophobicity with a water contact angle of 150.2° even after more than one hundred abrasion cycles, demonstrating the superior mechanical durability. Overall, this fabrication method has shown its effectiveness for fabrication of multifunctional metal surface integrating the surface functionalities of anti-reflectivity, superhydrophobicity/high oleophobicity, and enhanced abrasion resistance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma13245691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7764624PMC
December 2020

Study on a Rapid Imaging Method for Scanning Ion Conductance Microscopy Using a Double-Barreled Theta Pipette.

Anal Chem 2020 12 7;92(24):15789-15798. Epub 2020 Dec 7.

Key Laboratory of Education Ministry for Modern Design Rotor-Bearing System, Xi'an Jiaotong University, Xi'an 710049, China.

Scanning ion conductance microscopy (SICM) is a new noncontact, high-resolution scanning probe microscopy technique, which has become increasingly popular in recent years. The hopping mode-currently the most widely used scanning mode-can be used for imaging samples with complicated surface topographies. However, its slow scanning rate seriously restricts its broader application. This paper proposes a fast imaging control mode using a double-barreled theta pipette as the probe, which effectively increases the imaging rate. In this mode, sample surface height information is obtained when the double-barreled theta pipette approaches the sample in a two-step downward process. The ion current sum of two barrels and ion current of one barrel are used as feedback signals to approach the sample until the feedback signals decrease to the set threshold, respectively, thereby obtaining the height of the imaging point. First, this work used COMSOL to establish an SICM model and perform simulation analysis. The simulation results verified the proposed method's feasibility. Second, a scanning time mathematical model was established. The results revealed that the new method is superior to the traditional method in terms of imaging rate. Finally, experiments were performed on poly(dimethylsiloxane) (PDMS) samples using the two imaging modes described above. The results demonstrated that the new scanning mode could significantly improve the imaging rate of SICM without a loss in imaging quality and stability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.0c02840DOI Listing
December 2020

Systematic analysis of molecular mechanisms of heart failure through the pathway and network-based approach.

Life Sci 2021 Jan 28;265:118830. Epub 2020 Nov 28.

Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Provincial People's Hospital & Guangdong Academy of Medical Sciences, School of Medicine, South China University of Technology, Guangzhou, China. Electronic address:

Aims: The molecular networks and pathways involved in heart failure (HF) are still largely unknown. The present study aimed to systematically investigate the genes associated with HF, comprehensively explore their interactions and functions, and identify possible regulatory networks involved in HF.

Main Methods: The weighted gene coexpression network analysis (WGCNA), crosstalk analysis, and Pivot analysis were used to identify gene connections, interaction networks, and molecular regulatory mechanisms. Functional analysis and protein-protein interaction (PPI) were performed using DAVID and STRING databases. Gene set variation analysis (GSVA) and receiver operating characteristic (ROC) curve analysis were also performed to evaluate the relationship of the hub genes with HF.

Key Findings: A total of 5968 HF-related genes were obtained to construct the co-expression networks, and 18 relatively independent and closely linked modules were identified. Pivot analysis suggested that four transcription factors and five noncoding RNAs were involved in regulating the process of HF. The genes in the module with the highest positive correlation to HF was mainly enriched in cardiac remodeling and response to stress. Five upregulated hub genes (ASPN, FMOD, NT5E, LUM, and OGN) were identified and validated. Furthermore, the GSVA scores of the five hub genes for HF had a relatively high areas under the curve (AUC).

Significance: The results of this study revealed specific molecular networks and their potential regulatory mechanisms involved in HF. These may provide new insight into understanding the mechanisms underlying HF and help to identify more effective therapeutic targets for HF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2020.118830DOI Listing
January 2021

Image-based morphometric studies of human coronary artery bifurcations with/without coronary artery disease.

Comput Methods Biomech Biomed Engin 2020 Nov 30:1-17. Epub 2020 Nov 30.

Institute of Biomechanics, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, P.R. China.

It is of great clinical significance to study the relationship between coronary bifurcation's morphometrical feature change and coronary artery disease (CAD) lesion. The purpose of this study is to determine the morphological changes in patients with CAD lesion when compared with non-CAD subjects and to find indicators that may be used for cardiovascular disease diagnosis. Computed tomography angiography images from Southern Chinese populations were used to reconstruct three-dimensional coronary arterial trees. Murray's law was introduced to assess the level of deviation of the realistic vascular networks from their optimal state. The results showed CAD Left had the highest deviation values of ( and (), while non-CAD Right had the lowest values ( and respectively). Moreover, the slope values of the ratio between and for non-CAD Left, CAD Left, non-CAD Right, and CAD Right were 0.7428, 0.7004, 0.7628, and 0.7577, respectively. Theoretically, the slope value should equal to 1 when the bifurcation structure is in its optimal state. Therefore, these results indicated that coronary bifurcations with CAD lesion deviated from the optimal structure further than those without CAD lesion and coronary bifurcations in right were closer to the optimal structure than those in left. More importantly, the present study found that and depended only on the diseased state, but not age, suggesting that and were potentially used as two novel indicators for early CAD diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10255842.2020.1850702DOI Listing
November 2020

Lead-Free KNN-Based Textured Ceramics for High-Frequency Ultrasonic Transducer Application.

IEEE Trans Ultrason Ferroelectr Freq Control 2021 May 26;68(5):1979-1987. Epub 2021 Apr 26.

Environment-friendly lead-free piezoelectric materials with excellent piezoelectric properties are needed for high-frequency ultrasonic transducer applications. Recently, lead-free 0.915(KNaLi)NbO-0.075BaZrO 3-0.01(BiNa)TiO (KNLN-BZ-BNT) textured piezo- electric ceramics have high piezoelectric response, superior thermal stability, and excellent fatigue resistance, which are promising for devices applications. In this work, the KNLN-BZ-BNT textured ceramics were prepared by the tape-casting method. Microstructural morphology, phase transition, and electrical properties of KNLN-BZ-BNT textured ceramics were investigated. High-frequency needle-type ultrasonic transducers were designed and fabricated with these textured ceramics. The tightly focused transducers have a center frequency higher than 80 MHz and a -6-dB fractional bandwidth of 52%. Such transducers were built for an f -number close to 1, and the desired focal depth was achieved by press-focusing technology associated with a set of customer design fixture. Its lateral resolution was better than [Formula: see text] by scanning a 15- [Formula: see text] tungsten wire target. These promising results demonstrate that the lead-free KNLN-BZ-BNT textured ceramic is a good candidate for high-frequency ultrasonic transducer applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TUFFC.2020.3039120DOI Listing
May 2021

Revising ventricular septal defect residual shunts without aortic re-cross-clamping: a safe and effective surgical procedure.

Ann Transl Med 2020 Sep;8(18):1134

Department of Cardiac Surgery, Guangdong Provincial Cardiovascular Institute, Guangdong Provincial People's Hospital/Guangdong Academy of Medical Sciences, Guangzhou, China.

Background: The conventional approach to revising a residual shunt following ventricular septal defect (VSD) closure is to re-occlude the aorta and repair the residual shunt under cardioplegic arrest. The present study evaluated the safety and effectiveness of a new approach for revising residual shunts following VSD repair without re-occluding the aorta. This approach is known as on beating heart surgery.

Methods: This retrospective study included 80 pediatric patients who underwent surgical closure of a simple VSD. Residual shunts larger than 2 mm were intraoperatively detected by transesophageal echocardiography (TEE) and these patients received immediate reintervention. Of the patients, 37 received on beating heart surgery without aortic cross-clamping (Group A) and 43 patients were operated on with aortic cross-clamping and cardioplegia (Group B).

Results: Residual VSD closures were successfully performed in all patients. Group A had significantly shorter aortic cross-clamp times (P<0.0001), significantly shorter CPB times (P<0.01), a lower incidence of prolonged ventilation (>6 hours) (P=0.04), a lower incidence of prolonged intensive care unit (ICU) stay (ICU stay >1 day) (P=0.02), and reduced in-hospital expenses (P<0.0001) compared with Group B. There was no significant difference in the incidence of recurrent residual shunts (P=0.96), prolonged postoperative hospital stay (>5 days) (P=0.24), or the incidence of perioperative complications (P=0.81) between the groups.

Conclusions: On beating heart surgery is a safe and effective approach for the closure of residual VSDs and is significantly associated with a lower incidence of prolonged ventilation, a lower incidence of prolonged ICU stay, and reduced in-hospital expenses.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-20-5041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576084PMC
September 2020
-->