Publications by authors named "Jian Zhang"

6,918 Publications

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Multistructured Electrospun Nanofibers for Air Filtration: A Review.

ACS Appl Mater Interfaces 2021 May 11. Epub 2021 May 11.

Joint Laboratory of Advanced Biomedical Materials (NFU-UGent) College of Chemical Engineering Nanjing Forestry University (NFU), Nanjing 210037, P. R. China.

Air filtration materials (AFMs) have gradually become a research hotspot on account of the increasing attention paid to the global air quality problem. However, most AFMs cannot balance the contradiction between high filtration efficiency and low pressure drop. Electrospinning nanofibers have a large surface area to volume ratio, an adjustable porous structure, and a simple preparation process that make them an appropriate candidate for filtration materials. Therefore, electrospun nanofibers have attracted increased attention in air filtration applications. In this paper, first, the preparation methods of high-performance electrospun air filtration membranes (EAFMs) and the typical surface structures and filtration principles of electrospun fibers for air filtration are reviewed. Second, the research progress of EAFMs with multistructures, including nanoprotrusion, wrinkled, porous, branched, hollow, core-shell, ribbon, beaded, nets structure, and the application of these nanofibers in air filtration are summarized. Finally, challenges with the fabrication of EAFMs, limitations of their use, and trends for future developments are presented.
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http://dx.doi.org/10.1021/acsami.1c06520DOI Listing
May 2021

Homochiral metal-organic frameworks for enantioseparation.

Chem Soc Rev 2021 May 22;50(9):5706-5745. Epub 2021 Mar 22.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.

Obtaining homochiral compounds is of high importance to human health and environmental sustainability. Currently, enantioseparation is one of the most effective approaches to obtain homochiral compounds. Thanks to their controlled synthesis and high efficiency, homochiral metal-organic frameworks (HMOFs) are one of the most widely studied porous materials to enable enantioseparation. In this review, we discuss the chiral pocket model in depth as the key to unlock enantioselective separation mechanisms in HMOFs. In particular, we classify our discussion of these chiral pockets (also regarded as "molecular traps") into: (a) achiral/chiral linker based helical channels as a result of packing modality; and (b) chiral pores inherited from chiral ligands. Driven by a number of mechanisms of enantioseparation, conceptual advances have been recently made in the design of HMOFs for achieving high enantioseparation performances. Herein, these are systematically categorised and discussed. Further we elucidate various applications of HMOFs as regards enantioseparation, systematically classifying them into their use for purification and related analytical utility according to the reported examples. Last but not the least, we discuss the challenges and perspectives concerning the rational design of HMOFs and their corresponding enantioseparations.
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http://dx.doi.org/10.1039/d0cs01236jDOI Listing
May 2021

Effects of cadmium stress on growth and physiological characteristics of sassafras seedlings.

Sci Rep 2021 May 10;11(1):9913. Epub 2021 May 10.

Institute of Ecology and Forestry, College of Forestry, Sichuan Agricultural University, Chengdu, 611130, China.

The effects of cadmium stress on the growth and physiological characteristics of Sassafras tzumu Hemsl. were studied in pot experiments. Five Cd levels were tested [CT(Control Treatment) : 0 mg/kg, Cd5: 5 mg/kg, Cd20: 20 mg/kg, Cd50: 50 mg/kg, and Cd100: 100 mg/kg]. The growth and physiological characteristics of the sassafras seedlings in each level were measured. The results showed that soil Cd had negative influences on sassafras growth and reduced the net growth of plant height and the biomass of leaf, branch and root. Significant reductions were recorded in root biomass by 18.18%(Cd5), 27.35%(Cd20), 27.57%(Cd50) and 28.95%(Cd100). The contents of hydrogen peroxide decreased first then increased while malondialdehyde showed the opposite trend with increasing cadmium concentration. Decreases were found in hydrogen peroxide contents by 10.96%(Cd5), 11.82%(Cd20) and 7.02%(Cd50); increases were found in malondialdehyde contents by 15.47%(Cd5), 16.07%(Cd20) and 7.85%(Cd50), indicating that cadmium stress had a certain effect on the peroxidation of the inner cell membranes in the seedlings that resulted in damage to the cell membrane structure. Superoxide dismutase activity decreased among treatments by 17.05%(Cd5), 10,68%(Cd20), 20.85%(Cd50) and 8.91%(Cd100), while peroxidase activity increased steadily with increasing cadmium concentration; these results suggest that peroxidase is likely the main protective enzyme involved in the reactive oxygen removal system in sassafras seedlings. Upward trends were observed in proline content by 90.76%(Cd5), 74.36%(Cd20), 99.73%(Cd50) and 126.01%(Cd100). The increase in proline content with increasing cadmium concentration indicated that cadmium stress induced proline synthesis to resist osmotic stress in the seedlings. Compared to that in CT, the soluble sugar content declined under the different treatments by 32.84%(Cd5), 5.85%(Cd20), 25.55%(Cd50) and 38.69%(Cd100). Increases were observed in the soluble protein content by 2.34%(Cd5), 21.36%(Cd20), 53.15%(Cd50) and 24.22%(Cd100). At different levels of cadmium stress, the chlorophyll content in the seedlings first increased and then decreased, and it was higher in the Cd5 and Cd20 treatments than that in the CT treatment. These results reflected that cadmium had photosynthesis-promoting effects at low concentrations and photosynthesis-suppressing effects at high concentrations. The photosynthetic gas exchange parameters and photosynthetic light-response parameters showed downward trends with increasing cadmium concentration compared with those in CT; these results reflected the negative effects of cadmium stress on photosynthesis in sassafras seedlings.
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http://dx.doi.org/10.1038/s41598-021-89322-0DOI Listing
May 2021

Field synopsis and systematic meta-analyses of genetic association studies in neuromyelitis optica.

Autoimmun Rev 2021 May 7:102842. Epub 2021 May 7.

Department of Cell Biology, China Medical University, Shenyang, China. Electronic address:

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http://dx.doi.org/10.1016/j.autrev.2021.102842DOI Listing
May 2021

Field synopsis and systematic meta-analyses of genetic association studies in neuromyelitis optica.

Autoimmun Rev 2021 May 7:102843. Epub 2021 May 7.

Department of Cell Biology, China Medical University, Shenyang, China. Electronic address:

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http://dx.doi.org/10.1016/j.autrev.2021.102843DOI Listing
May 2021

Advances and Insights of APC-Asef Inhibitors for Metastatic Colorectal Cancer Therapy.

Front Mol Biosci 2021 22;8:662579. Epub 2021 Apr 22.

Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

In Colorectal cancer (CRC), adenomatous polyposis coli (APC) directly interacts with the Rho guanine nucleotide exchange factor 4 (Asef) and releases its GEF activity. Activated Asef promotes the aberrant migration and invasion of CRC cell through a CDC42-mediated pathway. Knockdown of either APC or Asef significantly decreases the migration of CRC cells. Therefore, disrupting the APC-Asef interaction is a promising strategy for the treatment of invasive CRC. With the growth of structural information, APC-Asef inhibitors have been designed, providing hope for CRC therapy. Here, we will review the APC-Asef interaction in cancer biology, the structural complex of APC-Asef, two generations of peptide inhibitors of APC-Asef, and small molecule inhibitors of APC-Asef, focusing on research articles over the past 30 years. We posit that these advances in the discovery of APC-Asef inhibitors establish the protein-protein interaction (PPI) as targetable and provide a framework for other PPI programs.
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http://dx.doi.org/10.3389/fmolb.2021.662579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100458PMC
April 2021

Allele-specific DNA methylation maps in monozygotic twins discordant for psychiatric disorders reveal that disease-associated switching at the EIPR1 regulatory loci modulates neural function.

Mol Psychiatry 2021 May 7. Epub 2021 May 7.

Department of Medical Genetics, School of Basic Medical Sciences, and Guangdong Technology and Engineering Research Center for Molecular Diagnostics of Human Genetic Diseases, Southern Medical University, Guangzhou, Guangdong, China.

The non-Mendelian features of phenotypic variations within monozygotic twins are likely complicated by environmental modifiers of genetic effects that have yet to be elucidated. Here, we performed methylome and genome analyses of blood DNA from psychiatric disorder-discordant monozygotic twins to study how allele-specific methylation (ASM) mediates phenotypic variations. We identified that thousands of genetic variants with ASM imbalances exhibit phenotypic variation-associated switching at regulatory loci. These ASMs have plausible causal associations with psychiatric disorders through effects on interactions between transcription factors, DNA methylations, and other epigenomic markers and then contribute to dysregulated gene expression, which eventually increases disease susceptibility. Moreover, we also experimentally validated the model that the rs4854158 alternative C allele at an ASM switching regulatory locus of EIPR1 encoding endosome-associated recycling protein-interacting protein 1, is associated with demethylation and higher RNA expression and shows lower TF binding affinities in unaffected controls. An epigenetic ASM switching induces C allele hypermethylation and then recruits repressive Polycomb repressive complex 2 (PRC2), reinforces trimethylation of lysine 27 on histone 3 and inhibits its transcriptional activity, thus leading to downregulation of EIPR1 in schizophrenia. Moreover, disruption of rs4854158 induces gain of EIPR1 function and promotes neural development and vesicle trafficking. Our study provides a powerful framework for identifying regulatory risk variants and contributes to our understanding of the interplay between genetic and epigenetic variants in mediating psychiatric disorder susceptibility.
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http://dx.doi.org/10.1038/s41380-021-01126-wDOI Listing
May 2021

A Comorbidity Knowledge-Aware Model for Disease Prognostic Prediction.

IEEE Trans Cybern 2021 May 7;PP. Epub 2021 May 7.

Prognostic prediction is the task of estimating a patient's risk of disease development based on various predictors. Such prediction is important for healthcare practitioners and patients because it reduces preventable harm and costs. As such, a prognostic prediction model is preferred if: 1) it exhibits encouraging performance and 2) it can generate intelligible rules, which enable experts to understand the logic of the model's decision process. However, current studies usually concentrated on only one of the two features. Toward filling this gap, in the present study, we develop a novel knowledge-aware Bayesian model taking into consideration accuracy and transparency simultaneously. Real-world case studies based on four years' territory-wide electronic health records are conducted to test the model. The results show that the proposed model surpasses state-of-the-art prognostic prediction models in accuracy and c-statistic. In addition, the proposed model can generate explainable rules.
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http://dx.doi.org/10.1109/TCYB.2021.3070227DOI Listing
May 2021

A population-based analysis of malignant gastric tumor in children.

J Pediatr Surg 2021 Apr 3. Epub 2021 Apr 3.

Department of Gastrointestinal Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Huansha Road 261, Hangzhou 31006, China. Electronic address:

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http://dx.doi.org/10.1016/j.jpedsurg.2021.03.056DOI Listing
April 2021

The functional impairment of different subtypes and occupational states in euthymic patients with bipolar disorder.

BMC Psychiatry 2021 May 7;21(1):240. Epub 2021 May 7.

Henan Key Laboratory of Neurorestoratology, the first Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.

Background: The aim was to explore the associations between clinical symptoms, demographic variables, social and neurocognitive functioning in euthymic patients with bipolar disorder (BD) stratified by subgroups of DSM-IV BD (type I (BD-I) and type II (BD-II)) and occupational status (employed/unemployed), and to highlight the significance of occupational status when assessing social and neurocognitive functioning in euthymic BD patients.

Methods: A total of 81 euthymic BD patients were participated in the study. The severity of the depressive and manic/hypomanic symptoms was measured using the 17-item Hamilton Depression Rating Scale (HDRS-17) and the Young Mania Rating Scale (YMRS), respectively. Social functioning and neurocognitive functioning were evaluated by the Functioning Assessment Short Test (FAST) and neurocognitive measures, respectively.

Results: Employed BD patients displayed greater social functioning (autonomy, occupational functioning, interpersonal relationship domain) and better verbal learning performance and speed of processing than unemployed BD patients. The correlation between neurocognitive functioning and social functioning was stronger in the employed group than in the unemployed group. There were no significant differences in neurocognitive and social functioning between the BD-I and BD-II groups, and the correlation between neurocognitive functioning and social functioning was similar between the BD-I and BD-II groups.

Conclusion: Employed BD patients may present greater occupational functioning and interpersonal relationships, as well as better verbal learning performance and speed of processing.
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http://dx.doi.org/10.1186/s12888-021-03242-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8103625PMC
May 2021

Evaluation of multi blade shear (MBS) for determining texture of raw and cooked broiler breast fillets with the woody breast myopathy.

Poult Sci 2021 Mar 12;100(6):101123. Epub 2021 Mar 12.

Poultry Sciences Department, University of Georgia, 110 Cedar St, Athens, GA, 30602, USA. Electronic address:

The objective of this study was to evaluate a novel multi-blade Shear (MBS) method for measuring texture properties of both raw and cooked broiler fillets (pectoralis major) with the woody breast (WB) myopathy. A total of 180 broiler breast fillets (60 normal [NOR], 60 moderate WB [MOD], and 60 severe WB [SEV]) in two meat states (fresh never-frozen, n = 144; frozen/thawed, n = 36) were chosen based on their WB scores. In each trial, half of the fillets were used for measuring raw meat texture and the other half for cooked meat texture measurement. Blunt Meullenet-Owens Razor Sear (BMORS) was used for comparison. In fresh raw broiler fillets, both the MBS and BMORS methods detected differences between NOR, MOD, and SEV fillets (P < 0.001). In cooked broiler fillets, the methods were equivalent in their ability to separate SEV from NOR fillets. The MBS measurements showed greater Spearman correlation coefficients with the WB scores (r ≥ 0.70 in raw and ≥ 0.33 in cooked) compared to the BMORS measurements (r = 0.63 in raw and ≤ 0.27 in cooked) for both fresh and cooked breast fillets. In addition, the MBS measurements were either as precise as or more precise than BMORS measurements regardless of meat condition (fresh vs. cooked) and the shear parameter. These results suggest that the MBS method is more reliable in measuring tactile characteristics of broiler breast fillets with the WB myopathy compared with the BMORS method.
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http://dx.doi.org/10.1016/j.psj.2021.101123DOI Listing
March 2021

An improved PD-AsLS method for baseline estimation in EDXRF analysis.

Anal Methods 2021 May;13(17):2037-2043

Chengdu University of Technology, Chengdu, Sichuan 610000, China. and Applied Nuclear Techniques in Geosciences Key Laboratory of Sichuan Province, Chengdu, Sichuan 610000, China.

Baseline correction is an important step in energy-dispersive X-ray fluorescence analysis. The asymmetric least squares method (AsLS), adaptive iteratively reweighted penalized least squares method (airPLS), and asymmetrically reweighted penalized least squares method (arPLS) are widely used to automatically select the data points for the baseline. Considering the parametric sensitivity of the aforementioned methods and the statistical characteristics of the X-ray energy spectrum, this paper proposes an asymmetrically reweighted penalized least squares method based on the Poisson distribution (PD-AsLS) to automatically correct the baseline of X-ray spectra. Monte Carlo (MC) simulation is used to obtain the background spectrum, and PD-AsLS is used to estimate the baseline of the background. The relative error and the absolute error between the simulated background and PD-AsLS estimated background are used to determine the accuracy of PD-AsLS. The correlation coefficient (COR) and the root mean square error (RMSE) between the estmated baseline and the real baseline are calculated, and results of PD-AsLS are compared with results of three other classical methods (arPLS, airPLS and AsLS) to evaluate the reliability of PD-AsLS. The results of PD-AsLS show that the COR is above 0.95 and RMSE is less than 6. The stability and the practicability of PD-AsLS are also evaluated in experiments. A sample is measured five time to get its X-ray energy spectra, and the coefficient of variation (CV) of the estimated baseline is smaller than that of measured spectra. Experiments show that PD-AsLS can estimate baselines better than arPLS without any overestimation. Those results indicate that PD-AsLS can reliably estimate the baselines of X-ray spectra and effectively suppress the statistical fluctuation.
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http://dx.doi.org/10.1039/d1ay00122aDOI Listing
May 2021

Venetoclax and decitabine in refractory TP53-mutated early T-cell precursor acute lymphoblastic leukemia.

Ann Hematol 2021 May 6. Epub 2021 May 6.

National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.

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http://dx.doi.org/10.1007/s00277-021-04530-yDOI Listing
May 2021

Genome-wide investigation of the AP2/ERF superfamily and their expression under salt stress in Chinese willow ().

PeerJ 2021 13;9:e11076. Epub 2021 Apr 13.

Lab of Landscape Plant Genetics and Breeding, School of Life Science, Nantong University, Nantong, Jiangsu, China.

AP2/ERF transcription factors (TFs) play indispensable roles in plant growth, development, and especially in various abiotic stresses responses. The AP2/ERF TF family has been discovered and classified in more than 50 species. However, little is known about the gene family of Chinese willow (), which is a tetraploid ornamental tree species that is widely planted and is also considered as a species that can improve the soil salinity of coastal beaches. In this study, 364 genes of () were identified depending on the recently produced whole genome sequencing data of . These genes were renamed according to the chromosomal location of the genes. The genes included three major subfamilies: AP2 (55 members), ERF (301 members), and RAV (six members) and two Soloist genes. Genes' structure and conserved motifs were analyzed in SmAP2/ERF family members, and introns were not found in most genes of the ERF subfamily, some unique motifs were found to be important for the function of genes. Syntenic relationships between the genes and genes from and showed that is genetically more closely related to than to . Evolution analysis on paralog gene pairs suggested that progenitor of originated from hybridization between two different diploid salix germplasms and underwent genome duplication not more than 10 Mya. RNA sequencing results demonstrated the differential expression patterns of some genes under salt stress and this information can help reveal the mechanism of salt tolerance regulation in .
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http://dx.doi.org/10.7717/peerj.11076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051338PMC
April 2021

Active-Ingredient Screening and Synergistic Action Mechanism of Shegan Mixture for Anti-Asthma Effects Based on Network Pharmacology in a Mouse Model of Asthma.

Drug Des Devel Ther 2021 29;15:1765-1777. Epub 2021 Apr 29.

Department of Pharmacy, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, 200092, People's Republic of China.

Background: Shegan Mixture (SGM) is a traditional Chinese medicine that has anti-inflammatory and therapeutic effects on asthma. However, its active ingredients and combined action mechanism have not been fully elucidated so far. The purpose of this study was to screen the effective ingredients and targets and elucidate the synergistic action mechanism of SGM in asthma mice using the network pharmacological approach.

Methods: A mouse model of asthma model was used in this study. Mice were orally administered SGM at three doses for 4 weeks and the effect of SGM on asthma was evaluated. The active ingredients and their targets of SGM were identified by searching databases, such as Traditional Chinese Medicine Systems Pharmacology Database (TCMSP). The main active ingredients were selected with parameters OB and DL. The synergistic action mechanisms of SGM in asthma were studied through key active ingredient-target interaction network and verified using surface plasmon resonance assay (SPR).

Results: SGM exerts anti-asthmatic effects by reducing lung tissue damage and inflammatory factors (IFN-γ, IL-4, IL-5, and IL-13) in asthmatic mice. Twenty ingredients and 45 related proteins were selected as potential nodes using enrichment analysis and network analysis. Inflammation and smooth muscle regulation-related pathways were considered to be the main pharmacological mechanisms of SGM in the treatment of asthma. Especially, 5 molecule-target pairs (including 3 ingredients and 4 proteins) were well docked with each other and the SPR assay revealed that glabridin-PTGS2 had good binding with 44.5 μM Kd value.

Conclusion: SGM exerts the synergistic anti-asthma effects by virtue of reducing lung-tissue damage and inflammatory factors in asthmatic mice, which explains the theoretical basis for the traditional Chinese medicine, SGM, to treat asthma. Our study thus sheds light on a variety of options including Chinese medicine that could potentially be used in the clinical treatment of asthma.
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http://dx.doi.org/10.2147/DDDT.S288829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092947PMC
April 2021

Three-Dimensional Self-Supporting TiC with MoS and CuO Nanocrystals for High-Performance Flexible Supercapacitors.

ACS Appl Mater Interfaces 2021 May 5. Epub 2021 May 5.

Guangxi Key Laboratory of Information Materials, Guilin University of Electronic Technology, Guilin 541004, P. R. China.

The three-dimensional (3D) architecture of electrode materials with excellent stability and electrochemical activity is extremely desirable for high-performance supercapacitors. In this study, we develop a facile method for fabricating 3D self-supporting TiC with MoS and CuO nanocrystal composites for supercapacitor applications. MoS was incorporated in TiC using a hydrothermal method, and CuO was embedded in two-dimensional nanosheets by in situ chemical reduction. The resulting composite electrode showed a synergistic effect between the components. TiC served as a conductive additive to connect MoS nanosheets and facilitate charge transfer. MoS acted as an active spacer to increase the interlayer space of TiC and protect TiC from oxidation. CuO effectively prevented the collapse of the lamellar structure of TiC-MoS. Consequently, the optimized composite exhibited an excellent specific capacitance of 1459 F g at a current density of 1 A g. Further, by assembling an all-solid-state flexible supercapacitor with activated carbon, a high energy density of 60.5 W h kg was achieved at a power density of 10 W kg. Additionally, the supercapacitor exhibited a capacitance retention of 90% during 3000 charging-discharging cycles. Moreover, high mechanical robustness was retained after bending at different angles, thereby suggesting significant potential applications for future flexible and wearable devices.
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http://dx.doi.org/10.1021/acsami.1c05231DOI Listing
May 2021

Association between dietary essential amino acids intake and metabolic biomarkers: influence of obesity among Chinese children and adolescents.

Amino Acids 2021 May 4. Epub 2021 May 4.

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, China.

Essential amino acids (EAAs) are involved in growth and development in children and adolescents. This study was aimed at exploring the relationship between dietary EAA intakes and metabolic biomarker, and the influence of obesity in children and adolescents. A total of 3566 subjects were analysed. Participators were classified according to weight status. Metabolic biomarkers were determined using standardized methods and conditions. Normal, overweight, and obesity statuses were defined according to the Working Group on Obesity in China (WGOC) BMI cutoff points based on age- and sex-specific screening criteria. In normal-weight group, blood uric acid was negatively correlated with dietary Ile, Leu, Lys, Phe, Thr, Val, and His, and zinc was negatively correlated with Ile, Leu, Lys, Phe, Thr, Val, His, Met, and Trp. In overweight group, TC was negatively correlated with Ile, Leu, Phe, Val, and His, and LDL-C was negatively correlated with Ile, Leu, Lys, Phe, Thr, Val, His, and Met, while TG was positively correlated with Leu, Lys, Phe, Thr, Val, and Met. In obesity group, hemoglobin was positively related to Ile, Leu, Lys, Phe, Thr, Val, His, and Trp, while vitamin D was positively correlated with His and Trp. The serum creatinine was negatively correlated with Ile, Leu, Phe, Val, His, and Met in normal-weight group, and positively correlated with Ile, Leu, Lys, Phe, Thr, Val, His, Met, Trp, His, and Trp in obesity group. Dietary amino acid score (AAS) and Leu intake were protective factors for obesity. The association between fasting blood glucose and EAAs intake was weak and labile. Metabolic biomarkers and EAA intakes were only related under certain weight status. The dietary AAS is positively correlated with HDL-C, LDL-C, serum creatinine, albumin, serum vitamin D, and zinc. The subtle relationship of EAAs and kidney function should be explored further. There is a complex relationship between EAAs and metabolic biomarkers, and overweight and obesity have a certain influence on this relationship.
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http://dx.doi.org/10.1007/s00726-021-02970-4DOI Listing
May 2021

Oxymatrine Extends Survival by Attenuating Neuroinflammation in a Mouse Model of Amyotrophic Lateral Sclerosis.

Neuroscience 2021 May 1;465:11-22. Epub 2021 May 1.

Department of Neurology, The Second Hospital of Hebei Medical University, 215 Heping West Road, Shijiazhuang, Hebei 050000, China; Beijing Geriatric Healthcare Center, Xuanwu Hospital, Capital Medical University, No. 45 Changchun Street, Beijing 100053, China. Electronic address:

Amyotrophic lateral sclerosis (ALS) is one of the leading causes of death associated with neurodegenerative diseases worldwide, and the progression of the disease is characteristically accompanied by severe neuroinflammation. Neuroprotective effects of oxymatrine (OMT) were shown to be due to reduced neuroinflammation in the mouse models of Alzheimer's disease and Parkinson's disease. The present study investigated whether OMT has a therapeutic potential in transgenic SOD1-G93A (TgSOD1-G93A) mice. Daily OMT treatment started at the age of 55 days until the end stage of the disease. Body weight and rotarod motor performance were assessed every 3 days starting from 70 days of age. Footprints were recorded to measure the stride length 40 days and 60 days after the initiation of the treatment. Some animals were sacrificed at the age of 115 days, and the lumbar spinal cord was harvested for immunofluorescence and quantitative real-time polymerase chain reaction (qRT-PCR) to evaluate the neuroinflammatory responses. The results indicated that treatment with OMT delayed body weight loss, improved motor performance, and prolonged the survival of SOD1-G93A mice. Mechanistically, OMT treatment enhanced motor neuronal survival and alleviated the activation of microglia and astrocytes compared with those in the vehicle-treated group. Furthermore, the expression of the proinflammatory mediators was downregulated, and the expression of the anti-inflammatory factors was upregulated in the OMT-treated group compared with those in the vehicle-treated group (P < 0.05). Thus, the treatment with OMT had neuroprotective effects, promoting neuronal survival and extending the lifetime of SOD1-G93A mice by suppressing neuroinflammation.
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http://dx.doi.org/10.1016/j.neuroscience.2021.04.019DOI Listing
May 2021

A Multitargeted Electrochemiluminescent Biosensor Coupling DNAzyme with Cascading Amplification for Analyzing Myocardial miRNAs.

Anal Chem 2021 May 4. Epub 2021 May 4.

School of Chemistry and Chemical Engineering, Anhui University of Technology, Ma Xiang Road, Ma'anshan, Anhui 243032, P. R. China.

Several circulating miRNAs are associated with the pathogenic process of acute myocardial infarction (AMI). Thus, analyzing myocardial miRNAs in the circulatory system is important for the diagnosis and treatment of AMI, especially for early-stage diagnosis. Based on the characteristics of myocardial miRNAs, an ultrasensitive and multitargeted electrochemiluminescence (ECL) sensing platform was developed with a versatile probe that can couple DNAzyme with hybridization chain reaction amplification. The target miRNA and auxiliary chains form a circular unit that shears the versatile probe hairpin, and the products subsequently trigger cascading amplification; a long strand of dsDNA is then generated with many C-rich sequences that can undergo in situ reductions to generate ECL luminophore silver clusters. Using this strategy, three myocardial miRNAs are successfully detected with a detection limit as low as 29.6 aM (/ = 3). Notably, our method can detect myocardial miRNA groups composed of multiple related circulating miRNAs with high selectivity over interfering miRNAs in blood. This is extremely important for solving the problem of diverse and low abundance of infarct-associated miRNAs. Our strategy pioneers a new idea of miRNA detection, and given its versatility and sensitivity, it is promising for the diagnosis of multigene-regulated cardiovascular diseases.
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http://dx.doi.org/10.1021/acs.analchem.1c01051DOI Listing
May 2021

Microphthalmia-Associated Transcription Factor in Senescence and Age-Related Diseases.

Gerontology 2021 May 3:1-10. Epub 2021 May 3.

Lab for Aging Research, National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, China.

Although microphthalmia-associated transcription factor (MITF) has been known for decades as a key regulator for melanocytic differentiation, recent studies expanded its other roles in multiple biological processes. Among these newfound roles, the relationship between MITF and aging is attractive; however, the underlying mechanism remains elusive. Here, we review the documented cues that highlight the implication of MITF in the aging process and particularly discuss the possible mechanisms underlying the participation of MITF in cellular senescence. First, it summarizes the association of MITF with melanocytic senescence, including the roles of MITF in cell cycle regulation, DNA damage repair, oxidative stress response, and the generation of senescence-associated secretory phenotype. Then, it collects the information involving MITF-related senescent changes in nonmelanocytes, such as retinal pigment epithelium cells, osteoclasts, and cardiomyocytes. This review may deepen the understanding of MITF function and be helpful to develop new strategies for improving geriatric health.
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http://dx.doi.org/10.1159/000515525DOI Listing
May 2021

Inhibition of 2-Arachidonoylglycerol Metabolism Alleviates Neuropathology and Improves Cognitive Function in a Tau Mouse Model of Alzheimer's Disease.

Mol Neurobiol 2021 May 3. Epub 2021 May 3.

Department of Cellular and Integrative Physiology, School of Medicine, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229, USA.

Alzheimer's disease (AD) is the most common cause of dementia, which affects more than 5 million individuals in the USA. Unfortunately, no effective therapies are currently available to prevent development of AD or to halt progression of the disease. It has been proposed that monoacylglycerol lipase (MAGL), the key enzyme degrading the endocannabinoid 2-arachidonoylglycerol (2-AG) in the brain, is a therapeutic target for AD based on the studies using the APP transgenic models of AD. While inhibition of 2-AG metabolism mitigates β-amyloid (Aβ) neuropathology, it is still not clear whether inactivation of MAGL alleviates tauopathies as accumulation and deposition of intracellular hyperphosphorylated tau protein are the neuropathological hallmark of AD. Here we show that JZL184, a potent MAGL inhibitor, significantly reduced proinflammatory cytokines, astrogliosis, phosphorylated GSK3β and tau, cleaved caspase-3, and phosphorylated NF-kB while it elevated PPARγ in P301S/PS19 mice, a tau mouse model of AD. Importantly, tau transgenic mice treated with JZL184 displayed improvements in spatial learning and memory retention. In addition, inactivation of MAGL ameliorates deteriorations in expression of synaptic proteins in P301S/PS19 mice. Our results provide further evidence that MAGL is a promising therapeutic target for AD.
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http://dx.doi.org/10.1007/s12035-021-02400-2DOI Listing
May 2021

A Novel Prognostic Model and Practical Nomogram for Predicting the Outcomes of Colorectal Cancer: Based on Tumor Biomarkers and Log Odds of Positive Lymph Node Scheme.

Front Oncol 2021 16;11:661040. Epub 2021 Apr 16.

State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.

Background: Emerging evidence shows that serum tumor biomarkers (TBs) and log odds of positive lymph node scheme (LODDS) are closely associated with the prognosis of colorectal cancer (CRC) patients. The aim of our study is to validate the predictive value of TBs and LODDS clinically and to develop a robust prognostic model to predict the overall survival (OS) of patients with CRC.

Methods: CRC patients who underwent radical resection and with no preoperative chemotherapy were enrolled in the study. The eligible population were randomized into training (70%) and test (30%) cohorts for the comprehensive evaluation of the prognostic model. Clinical implications of serum biomarkers and LODDS were identified by univariate and multivariate Cox proportion regression analysis. The predictive ability and discriminative performance were evaluated by Kaplan-Meier (K-M) curves and receiver operating characteristic (ROC) curves. Clinical applicability of the prognostic model was assessed by decision curve analysis (DCA), and the corresponding nomogram was constructed based on the above factors.

Results: A total of 1,202 eligible CRC patients were incorporated into our study. Multivariable COX analysis demonstrated that CA199 (HR = 1.304), CA125 (HR = 1.429), CEA (HR = 1.307), and LODDS (HR = 1.488) were independent risk factors for OS (all P < 0.0001). K-M curves showed that the high-risk group possessed a shorter OS than the low-risk counterparts. The area under curves (AUCs) of the model for 1-, 3- and 5-year OS were 86.04, 78.70, and 76.66% respectively for the train cohort (80.35, 77.59, and 74.26% for test cohort). Logistic DCA and survival DCA confirmed that the prognostic model displayed more clinical benefits than the conventional AJCC 8 TNM stage and CEA model. The nomograms were built accordingly, and the calibration plot for the probability of survival at 3- or 5-years after surgery showed an optimal agreement between prediction and actual observation.

Conclusions: Preoperative serum TBs and LODDS have significant clinical implications for CRC patients. A novel prognostic model incorporating common TBs (CA199, CA125, and CEA) and LODDS displayed better predictive performance than both single factor and the TNM classification. A novel nomogram incorporating TBs and LODDS could individually predict OS in patients with CRC.
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http://dx.doi.org/10.3389/fonc.2021.661040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085421PMC
April 2021

Comparison of Diffusion Kurtosis Imaging and Amide Proton Transfer Imaging in the Diagnosis and Risk Assessment of Prostate Cancer.

Front Oncol 2021 15;11:640906. Epub 2021 Apr 15.

Department of MR, The First Affiliated Hospital, Xinxiang Medical University, Weihui, China.

Objectives: This study aims to evaluate and compare the diagnostic value of DKI and APT in prostate cancer (PCa), and their correlation with Gleason Score (GS).

Materials And Methods: DKI and APT imaging of 49 patients with PCa and 51 patients with benign prostatic hyperplasia (BPH) were collected and analyzed, respectively. According to the GS, the patients with PCa were divided into high-risk, intermediate-risk and low-risk groups. The mean kurtosis (MK), mean diffusion (MD) and magnetization transfer ratio asymmetry (MTRasym, 3.5 ppm) values among PCa, BPH, and different GS groups of PCa were compared and analyzed respectively. The diagnostic accuracy of each parameter was evaluated by using the receiver operating characteristic (ROC) curve. The correlation between each parameter and GS was analyzed by using Spearman's rank correlation.

Results: The MK and MTRasym (3.5 ppm) values were significantly higher in PCa group than in BPH group, while the MD value was significantly lower than in BPH group. The differences of MK/MD/MTRasym (3.5 ppm) between any two of the low-risk, intermediate-risk, and high-risk groups were all statistically significant (p <0.05). The MK value showed the highest diagnostic accuracy in differentiating PCa and BPH, BPH and low-risk, low-risk and intermediate-risk, intermediate-risk and high-risk (AUC = 0.965, 0.882, 0.839, 0.836). The MK/MD/MTRasym (3.ppm) values showed good and moderate correlation with GS ( = 0.844, -0.811, 0.640, p <0.05), respectively.

Conclusion: DKI and APT imaging are valuable in the diagnosis of PCa and demonstrate strong correlation with GS, which has great significance in the risk assessment of PCa.
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http://dx.doi.org/10.3389/fonc.2021.640906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082407PMC
April 2021

Photoacoustic imaging as a highly efficient and precise imaging strategy for the evaluation of brain diseases.

Quant Imaging Med Surg 2021 May;11(5):2169-2186

Department of Biomedical Engineering, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China.

Photoacoustic imaging (PAI) is an emerging imaging strategy with a unique combination of rich optical contrasts, high ultrasound spatial resolution, and deep penetration depth without ionizing radiation. Taking advantage of the features mentioned above, PAI has been widely applied to preclinical studies in diverse fields, such as vascular biology, cardiology, neurology, ophthalmology, dermatology, gastroenterology, and oncology. Among various biomedical applications, photoacoustic brain imaging has great importance due to the brain's complex anatomy and the variability of brain disease. In this review, we aimed to introduce a novel and effective imaging modality for diagnosing brain diseases. Firstly, a brief overview of two major types of PAI system was provided. Then, PAI's major preclinical applications in brain diseases were introduced, including early diagnosis of brain tumors, subtle changes in the chemotherapy response, epileptic activity and brain injury, foreign body, and brain plaque. Finally, a perspective of the remaining challenges of PAI was given for future advancements.
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http://dx.doi.org/10.21037/qims-20-845DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047382PMC
May 2021

A preliminary study identifies early postoperative lung volume changes in patients with non-small cell lung cancer following video-assisted thoracic surgery using CT volumetry.

Mol Clin Oncol 2021 Jun 22;14(6):124. Epub 2021 Apr 22.

Department of Thoracic Surgery, The Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563099, P.R. China.

The present study aimed to investigate the changes in early postoperative lung volume in patients with non-small cell lung cancer (NSCLC) following video-assisted thoracic surgery (VATS) and to analyze the effects of the clinical characteristics on the lung volume of the patients. Therefore, 38 patients with NSCLC, who planned to undergo VATS at the Department of Thoracic Surgery, The Affiliated Hospital of Guizhou Medical University in June 2019, were enrolled into the present study. The clinical and computed tomography (CT) scan data from the patients was prospectively collected within 1 week preoperatively, and at 1, 3 and 6 months following surgery, then subsequently analyzed. A total of 34 patients successfully completed follow-up and were included in the datasets. The results showed that the volume of the right lung was larger compared with that in the left one, at each observational time point. The whole, right and left lung held the same trendline of volume changes, which was sharply decreased during the first postoperative month, increased quickly over the next 3 months, and slowly increased from months 3 to 6. There were 7 patients, whose whole lung volume was increased at 6 months following surgery compared with that preoperatively. In addition, significant differences were observed between males and females in the whole, right and left lung volume. However, the differences on the postoperative net expansion volume of the whole lung were not significant among sex, age, body mass index (BMI), smoking status and surgical side subgroups. The early changes of the postoperative lung volume were not linear, since the lung volume was significantly reduced during the first postoperative month, quickly increased in the next 3 months, and slowly increased from months 3 to 6. Sex, age, BMI, smoking status and surgical sides was not found to affect the postoperative volume and net expansion of the whole lung following VATS lobectomy.
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http://dx.doi.org/10.3892/mco.2021.2286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082225PMC
June 2021

Functional consequences of a rare missense BARD1 c.403G>A germline mutation identified in a triple-negative breast cancer patient.

Breast Cancer Res 2021 May 1;23(1):53. Epub 2021 May 1.

State Key Laboratory of Genetic Engineering, School of Life Sciences and Shanghai Cancer Center, Fudan University, Shanghai, China.

We identified a rare missense germline mutation in BARD1 (c.403G>A or p.Asp135Asn) as pathogenic using integrated genomics and transcriptomics profiling of germline and tumor samples from an early-onset triple-negative breast cancer patient who later was administrated with a PARP inhibitor for 2 months. We demonstrated in cell and mouse models that, compared to the wild-type, (1) c.403G>A mutant cell lines were more sensitive to irradiation, a DNA damage agent, and a PARP inhibitor; (2) c.403G>A mutation inhibited interaction between BARD1 and RAD51 (but not BRCA1); and (3) c.403G>A mutant mice were hypersensitive to ionizing radiation. Our study shed lights on the clinical interpretation of rare germline mutations of BARD1.
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http://dx.doi.org/10.1186/s13058-021-01428-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088670PMC
May 2021

Phosphate-solubilizing bacterium Burkholderia sp. strain N3 facilitates the regulation of gene expression and improves tomato seedling growth under cadmium stress.

Ecotoxicol Environ Saf 2021 Jul 28;217:112268. Epub 2021 Apr 28.

Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230031 Anhui Province, China.

Cadmium (Cd) is among the most toxic heavy metals in soils. The ways by which tomato plants inoculated with a phosphate-solubilizing bacterium (PSB) respond to Cd and regulate gene expression remain unclear. We investigated hormone metabolism and genes involved in Cd resistance in tomato seedlings inoculated with the PSB strain N3. Cd inhibited tomato plant growth and nutrient uptake and increase in dry weight. Compared with Cd treatment, N3 inoculation inhibited the accumulation of Cd in the shoots and roots, and the root dry weight significantly increased by 30.50% (P < 0.05). The nitrogen and potassium contents in the roots of seedlings treated with N3 increased, and the phosphorus levels were the same as those in the control. N3 decreased the rate of Zn absorption but increased Fe absorption in the roots, and the amount of accumulated Cd increased with Zn uptake. The concentrations of hormones (indole-3-acetic acid, IAA; zeatin, ZEA; and jasmonic acid, JA) increased under Cd stress, whereas inoculation with N3 reduced IAA and ZEA levels. In the comparison between N3 + Cd and Cd treatments, the highest number of up- and downregulated genes was obtained. Pathways involved in signaling response, photosynthesis, phenylpropanoid biosynthesis, and DNA replication and the photosynthesis-antenna proteins pathway play important roles in the responses and adaptation of seedlings to Cd. Inoculation with N3 alleviates Cd stress in tomato seedlings. The present study provides new insights into the differentially expressed genes related to interaction between PSB and tomato exposed to Cd in soils.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112268DOI Listing
July 2021

The Ghd7 Transcription Factor Represses the ARE1 Expression to Enhance Nitrogen Utilization and Grain Yield in Rice.

Mol Plant 2021 Apr 27. Epub 2021 Apr 27.

State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Beijing 100101, China; Hainan Yazhou Bay Seed Laboratory, Sanya, 572025, China. Electronic address:

The genetic improvement of nitrogen use efficiency (NUE) of crops is vital for grain productivity and sustainable agriculture. However, the regulatory mechanism of NUE remains largely elusive. Here, we find that the rice Grain number, plant height, and heading date7 (Ghd7) gene genetically acts upstream of ABC1 REPRESSOR1 (ARE1), a negative regulator of NUE, to positively regulate nitrogen utilization. As a transcriptional repressor, Ghd7 directly binds to two Evening Element-like motifs in the promoter and Intron 1 of ARE1, likely in a cooperative manner, to repress its expression. Ghd7 and ARE1 display diurnal expression patterns in an inverse oscillation manner, mirroring a regulatory scheme based on these two loci. Analysis of a panel of 2,656 rice varieties suggests that the elite alleles of Ghd7 and ARE1 have undergone diversifying selection during breeding. Moreover, the allelic distribution of Ghd7 and ARE1 is associated with the soil nitrogen deposition rate in East Asia and South Asia. Remarkably, the combination of the Ghd7 and ARE1 elite alleles substantially improves NUE and yield performance under nitrogen-limiting conditions. These results define a Ghd7-ARE1-based mechanism regulating nitrogen utilization, which may represent useful targets for the genetic improvement of rice nitrogen utilization in breeding.
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http://dx.doi.org/10.1016/j.molp.2021.04.012DOI Listing
April 2021

Synthesis, Structures, and Fluorescence Properties of Dimeric Aluminum Oxo Clusters.

Inorg Chem 2021 Apr 29. Epub 2021 Apr 29.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.

Aluminum is an important component for luminescence. However, the fluorescent aluminum complex with unambiguous structural information is still limited. Herein, we report a series of fluorescence aluminum oxo clusters (AlOCs). By introducing an additional coordination site to the aromatic conjugation ligand, cluster nuclearity increment and fluorescence variation are observed. Al(OH)(μ-O)(1-NA)(OEt) (, 1-NA = 1-naphthoic acid, OEt = ethanol) is made up of two tetrahedral subunits. By introducing an additional coordination site to the aromatic conjugation ligand, we isolate a high nuclearity compound Al(μ-O)(3-HNA)(OEt) (, 3-HNA = 3-hydroxy-2-naphthoic acid). Correspondingly, their luminescence performance is different (blue fluorescence in and green in ). Present herein is a platform to illustrate the relationship between synthesis, structure, and fluorescence properties.
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http://dx.doi.org/10.1021/acs.inorgchem.0c03816DOI Listing
April 2021