Publications by authors named "Jian Yu"

1,050 Publications

  • Page 1 of 1

Analysis of the Clinical Effects of Sodium Valproate and Levetiracetam in the Treatment of Women with Epilepsy during Pregnancy.

Evid Based Complement Alternat Med 2021 29;2021:5962200. Epub 2021 Sep 29.

Department of Pharmacy, Zhuji Maternal and Child Health Hospital, Shaoxing 311800, Zhejiang, China.

Objective: To explore the clinical effects of sodium valproate and levetiracetam in the treatment of women with epilepsy during pregnancy.

Methods: The clinical data of 124 women with epilepsy during pregnancy who received monotherapy with antiepileptic drugs (AEDs) in our hospital from September 2017 to January 2020 were retrospectively analyzed. According to the type of medication taken by the patients, they were recorded as the sodium valproate group (the VPA group,  = 56) and the levetiracetam group (the LEV group,  = 68 cases). The effects and the maternal and infant outcomes after treatment were compared between the two groups. The neuron-specific enolase (NSE), cognitive function-related parameters (brain-derived neurotrophic factor (BDNF) and myelin basic protein (MBP)), and related inflammatory factors (tumor necrosis factor- (TNF-) and interleukin- (IL-) 6) levels were compared between the two groups before and after treatment.

Results: After treatment, the total clinical effective rate of the LEV group was 91.18% higher than that of the VPA group 73.21%, and the frequency and duration of seizures were lower than those of the VPA group ( < 0.05). After treatment, the probability of gestational hypertension, depression during pregnancy, low-weight infants, and neonatal deformities in the LEV group was lower than that in the VPA group ( < 0.05). After treatment, the levels of NSE, MBP, TNF-, and IL-6 in the two groups decreased, and the levels of BDNF increased, and the LEV group changed significantly compared with the VPA group ( < 0.05).

Conclusion: Compared with sodium valproate monotherapy, levetiracetam is more effective in controlling seizures and improving maternal and infant outcomes in women with epilepsy during pregnancy and can effectively regulate their neurological and cognitive functions and reduce the serum inflammation factor level.
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http://dx.doi.org/10.1155/2021/5962200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494592PMC
September 2021

Generation of an induced pluripotent stem cell line (FDCHi006-A) from a 7-year-old girl with central precocious puberty.

Stem Cell Res 2021 Sep 28;56:102542. Epub 2021 Sep 28.

Department of Traditional Chinese Medicine, Children's Hospital of Fudan University, Shanghai ty 201102, China. Electronic address:

Central precocious puberty (CPP) refers to a pediatric endocrine disease in which boys have secondary sexual characteristics before the age of 9 and girls before the age of 8. In this study, we generated an induced pluripotent stem cell line FDCHi006-A from the Peripheral blood cell of a typical central precocious patient who was only 7 years old. This iPSC line showed full pluripotency, normal karyotype and differentiation capacity without integrating vectors. It provides a useful model to study the underlying molecular mechanisms of CPP.
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http://dx.doi.org/10.1016/j.scr.2021.102542DOI Listing
September 2021

BET protein degradation triggers DR5-mediated immunogenic cell death to suppress colorectal cancer and potentiate immune checkpoint blockade.

Oncogene 2021 Oct 6. Epub 2021 Oct 6.

UPMC Hillman Cancer Center, Pittsburgh, PA, 15213, USA.

Bromodomain and extra-terminal domain (BET) family proteins are epigenetic readers that play a critical role in oncogenesis by controlling the expression of oncogenes such as c-Myc. Targeting BET family proteins has recently emerged as a promising anticancer strategy. However, the molecular mechanisms by which cancer cells respond to BET inhibition are not well understood. In this study, we found that inducing the degradation of BET proteins by the proteolysis targeting chimeras (PROTAC) approach potently suppressed the growth of colorectal cancer (CRC) including patient-derived tumors. Mechanistically, BET degradation transcriptionally activates Death Receptor 5 (DR5) to trigger immunogenic cell death (ICD) in CRC cells. Enhanced DR5 induction further sensitizes CRC cells with a mutation in Speckle-type POZ protein (SPOP). Furthermore, DR5 is indispensable for a striking antitumor effect of combining BET degradation and anti-PD-1 antibody, which was well tolerated in mice and almost eradicated syngeneic tumors. Our results demonstrate that BET degradation triggers DR5-mediated ICD to potently suppress CRC and potentiate immune checkpoint blockade. These results provide a rationale, mechanistic insights, and potential biomarkers for developing a precision CRC therapy by inducing BET protein degradation.
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http://dx.doi.org/10.1038/s41388-021-02041-8DOI Listing
October 2021

Interferon b drives intestinal regeneration after radiation.

Sci Adv 2021 Oct 6;7(41):eabi5253. Epub 2021 Oct 6.

Department of Pathology, University of Pittsburgh School of Medicine, UPMC Hillman Cancer Center, Pittsburgh, PA 15213, USA.

[Figure: see text].
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http://dx.doi.org/10.1126/sciadv.abi5253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494436PMC
October 2021

Polyurethane-Based Face Mask as a Sampling Device for Environmental Tobacco Smoke.

Anal Chem 2021 Oct 5. Epub 2021 Oct 5.

Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.

Environmental tobacco smoke (ETS), also known as secondhand smoking, contains human carcinogens associated with the development of many human diseases, including stroke, heart disease, leukemia, and lung cancer. Due to these adverse health effects, a sensitive and selective method is crucial for assessing the health impacts of ETS. While current methods to evaluate ETS exposure are either invasive or nonspecific and insensitive, in this study, we assessed the use of polyurethane foam face masks as a sampling medium to collect tobacco smoke-specific nicotine and nitrosamines for estimating personal exposure to ETS. This method was used in conjunction with tandem mass spectrometry coupled with isotope-dilution detection. After validation by comparison with the National Institute for Occupational Safety and Health standard method (NIOSH 2551) for nicotine, we quantitated ETS exposure in indoor and outdoor environments. The analysis shows the applicability of the method for monitoring nicotine down to ∼0.20 mg/m near an outdoor smoking hotspot and up to ∼5.2 mg/m in a room with burning cigarettes, all with a time resolution as short as 5 min. In comparison with the NIOSH method, the newly developed method is convenient, inexpensive, and does not require a personal sampling pump, thus can facilitate large-scale ETS exposure monitoring.
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http://dx.doi.org/10.1021/acs.analchem.1c02906DOI Listing
October 2021

Internal Limiting Membrane Peeling and Flap Inverting under Air in Large Idiopathic Macular Hole Surgery.

J Ophthalmol 2021 24;2021:2003001. Epub 2021 Sep 24.

Department of Ophthalmology and Vision Science, Eye and ENT Hospital, Fudan University, Shanghai 200031, China.

Purpose: To evaluate the efficacy of internal limiting membrane (ILM) peeling combined with modified flap inverting under air in the treatment of large idiopathic macular hole (MH).

Methods: Eyes with a large idiopathic MH (minimum diameter >550 m) were included in this study. The surgical procedure included standard 23-gauge pars plana vitrectomy (PPV), ILM peeling, complete fluid-gas exchange, and ILM flap inversion under air. The patients underwent follow-up exam including optical coherence tomography (OCT) and best-corrected visual acuity (BCVA) measurement.

Results: Sixteen eyes from 16 patients were included. Mean MH diameter was 681.43 ± 112.12 m. After a mean follow-up time of 6.25 ± 2.65 months, in all cases, the MH was closed, and the ILM flap could be seen at the inner surface of the fovea. U-shaped and V-shaped MH closure was achieved in 11 and 5 cases, respectively. The BCVA improved significantly from 1.49 ± 0.35 logMAR to 0.89 ± 0.35 logMAR ( < 0.05), and visual acuity of 20/100 or better was achieved in 8 eyes.

Conclusion: ILM flap inverting under air was helpful in improving the functional and anatomic outcomes of vitrectomy for large idiopathic MH.
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http://dx.doi.org/10.1155/2021/2003001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8487370PMC
September 2021

On the Flip Side of Mask Wearing: Increased Exposure to Volatile Organic Compounds and a Risk-Reducing Solution.

Environ Sci Technol 2021 Oct 1. Epub 2021 Oct 1.

Department of Chemistry and Division of Environment, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.

Surgical masks have been worn by the public worldwide during the COVID-19 pandemic, yet hazardous chemicals in the petroleum-derived polymer layer of masks are currently ignored and unregulated. These organic compounds pose potential health risks to the mask wearer through dermal contact or inhalation. Here, we show that surgical masks from around the world are loaded with semivolatile and volatile organic compounds (VOCs), including alkanes, polycyclic aromatic hydrocarbons (PAHs), phthalate esters, and reactive carbonyls at ng to μg/mask levels. Naphthalene was the most abundant mask-borne PAH, accounting for over 80% of total PAH levels; acrolein, a mutagenic carbonyl, was detected in most of the mask samples, and di(2-ethylhexyl) phthalate, an androgen antagonist, was detected in one-third of the samples. Furthermore, there is large mask-to-mask variability of the residue VOCs, revealing the uneven quality of masks. We confirm that masks containing more residue VOCs lead to significantly higher exposure levels and associated disease risks to the wearer, which should warrant the attention of the general public and regulatory agencies. We find that heating the masks at 50 °C for as short as 60 min lowers the total VOC content by up to 80%, providing a simple method to limit our exposure to mask-borne VOCs.
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http://dx.doi.org/10.1021/acs.est.1c04591DOI Listing
October 2021

[email protected] derived porous carbon-supported MoO/MoS octahedra boosting high-rate lithium storage.

Dalton Trans 2021 Sep 30. Epub 2021 Sep 30.

School of Materials Science and Engineering, University of Jinan, Jinan, 250022, P.R. China.

Structural stability and rapid charge-discharge capability of electrode materials are required for high performance lithium-ion batteries (LIBs). The materials derived from polyoxometalates (POMs) show special advantages in inhibiting capacity attenuation, and good dispersion or combination of POMs with metal-organic frameworks (MOFs) is an important method to obtain high activity anode composites for LIBs. In this study, a uniform MoO/MoS heterostructure with surface supported carbon (C-MoO/MoS) was successfully fabricated from a [Cu(BTC)(HO)][HPMoO] precursor, which showed not only the designed octahedral morphology but also fast charge transfer, long working life, and high rate performance. Superior reversible lithium storage capacity of 1047 mA h g after 300 cycles was obtained at 1 A g. Even after 700 cycles at 5 A g, the discharge specific capacity of 646 mA h g was maintained, and rate capability of 610 mA h g could be achieved at 10 A g. The high capacitive contribution could be explained by the relatively large specific surface area of porous C-MoO/MoS, which was mainly caused by the supported carbon network and MoS nanosheets, resulting in fast lithiation/delithiation processes.
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http://dx.doi.org/10.1039/d1dt02475bDOI Listing
September 2021

Paired heavy- and light-chain signatures contribute to potent SARS-CoV-2 neutralization in public antibody responses.

Cell Rep 2021 10 28;37(1):109771. Epub 2021 Sep 28.

Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305, USA.

Understanding mechanisms of protective antibody recognition can inform vaccine and therapeutic strategies against SARS-CoV-2. We report a monoclonal antibody, 910-30, targeting the SARS-CoV-2 receptor-binding site for ACE2 as a member of a public antibody response encoded by IGHV3-53/IGHV3-66 genes. Sequence and structural analyses of 910-30 and related antibodies explore how class recognition features correlate with SARS-CoV-2 neutralization. Cryo-EM structures of 910-30 bound to the SARS-CoV-2 spike trimer reveal binding interactions and its ability to disassemble spike. Despite heavy-chain sequence similarity, biophysical analyses of IGHV3-53/3-66-encoded antibodies highlight the importance of native heavy:light pairings for ACE2-binding competition and SARS-CoV-2 neutralization. We develop paired heavy:light class sequence signatures and determine antibody precursor prevalence to be ∼1 in 44,000 human B cells, consistent with public antibody identification in several convalescent COVID-19 patients. These class signatures reveal genetic, structural, and functional immune features that are helpful in accelerating antibody-based medical interventions for SARS-CoV-2.
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http://dx.doi.org/10.1016/j.celrep.2021.109771DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479507PMC
October 2021

Solution-shearing of dielectric polymer with high thermal conductivity and electric insulation.

Sci Adv 2021 Oct 29;7(40):eabi7410. Epub 2021 Sep 29.

Department of Mechanical and Aerospace Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.

[Figure: see text].
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http://dx.doi.org/10.1126/sciadv.abi7410DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8480926PMC
October 2021

Detection sensitivity of ultrasound scanning vs. clinical examination for insulin injection-related lipohypertrophy.

Chin Med J (Engl) 2021 Sep 21. Epub 2021 Sep 21.

Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University (Jiangsu Province Hospital), Nanjing, Jiangsu 210029, China Department of Radiology and Oncology, The First Affiliated Hospital of Nanjing Medical University (Jiangsu Province Hospital), Nanjing, Jiangsu 210029, China School of Nursing, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.

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http://dx.doi.org/10.1097/CM9.0000000000001742DOI Listing
September 2021

Evaluation of resveratrol-doped adhesive with advanced dentin bond durability.

J Dent 2021 Sep 21;114:103817. Epub 2021 Sep 21.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China. Electronic address:

Objectives: This paper aimed to evaluate the influence of resveratrol-doped adhesive on the durability and antibiofilm capability of dentin bonding.

Methods: Experimental adhesives were prepared by incorporating resveratrol into a universal adhesive at concentrations of 0 (control), 0.1, 1, and 10 mg/mL. The microtensile bond strength, fracture modes, and adhesive-dentin interface nanoleakage were assessed after 24 h of water storage, 10,000 times of thermocycling or 1-month of collagenase ageing. Relevant antibiofilm capability on Streptococcus mutans (S. mutans), in situ zymography, degree of conversion, and cytotoxicity of resveratrol-doped adhesives were also determined.

Results: Irrespective of thermocycled or collagenase ageing, the resveratrol-doped adhesive (1 mg/mL) maintained the bond strength and reduced the nanoleakage expression. Meanwhile, the inhibitory ability on endogenous protease activity and S. mutans biofilm formation with acceptable biocompatibility were obtained.

Conclusions: This study suggested that the resveratrol-doped adhesive achieved effective improvement on dentin bond durability and secondary caries management.

Clinical Significance: The application of the resveratrol-doped adhesive indicates promising benefits to increase the lifetime of composite restorations.
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http://dx.doi.org/10.1016/j.jdent.2021.103817DOI Listing
September 2021

Effects of N-Methyl-D-aspartate receptor (NMDAR) and Ca/calmodulin-dependent protein kinase IIα (CaMKIIα) on learning and memory impairment in depressed rats with different charge by modified electroconvulsive shock.

Ann Transl Med 2021 Aug;9(16):1320

Department of Anesthesiology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: With the development of modified electroshock therapy (MECT), it has become necessary to increase the electric quantity in order to achieve a good antidepressant effect, but this increase will lead to more serious learning and memory impairment. The purpose of this study was to investigate the intrinsic mechanism of cognitive impairment induced by high-energy electroconvulsive shock (MECS, an animal model of MECT).

Methods: Rats were randomly divided into 6 groups: control (C, n=6), M0, M60, M120, M180, and M240 groups (MECS at 0, 60, 120, 180, and 240 mC stimulation intensity after 80 mg/kg propofol, with 12 rats in each group). Their depression-like behavior and learning and memory ability were evaluated by sucrose preference test (SPT), open field test (OFT), and Morris water maze test (MWM). The expression of phospho-NMDA receptor 1 (GluN1), GluN2A, GluN2B, Ca/calmodulin-dependent protein kinase IIα (CaMKIIα), p-T305-CaMKII, and postsynaptic densities-95 (PSD-95) in hippocampus were detected by western blot. The co-expression of CaMKIIα and GluN2B subunit was detected by co-immunoprecipitation (CO-IP).

Results: The chronic unpredictable mild stresses (CUMS) procedure successfully induced depression-like behavior in rats, which was improved in varying degrees after MECS. The results showed that the expression of GluN1, GluN2A, GluN2B, and PSD-95 decreased with the increase of charge, while p-T305-CaMKII increased, which led to the deterioration of learning and memory ability, but the expression change of CaMKIIα was not statistically significant.

Conclusions: Increase in the MECS charge adjusts the synaptic plasticity by changing the binding amount of CaMKIIα and its subunit GluN2B and the level of CaMKII autophosphorylation, thereby impairing learning and memory functions.
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http://dx.doi.org/10.21037/atm-21-3690DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422109PMC
August 2021

Estimating primary vehicular emission contributions to PM using the Chemical Mass Balance model: Accounting for gas-particle partitioning of organic aerosols and oxidation degradation of hopanes.

Environ Pollut 2021 Sep 8;291:118131. Epub 2021 Sep 8.

Division of Environment & Sustainability, Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong; Department of Chemistry, Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong. Electronic address:

Particulate matter emitted from vehicles (PM) represents a major air pollution source in urban areas. Ambient measurements of hopanes and elemental carbon have traditionally been coupled with the Chemical Mass Balance (CMB) model to quantify the contributions to fine PM from diesel and gasoline vehicular emissions (VE). The organic carbon part of PM, however, undergoes gas-particle partitioning and oxidation degradation as VE move from exhaust pipe to receptor sites. This creates an issue of deviation from mass conservation in the utility of CMB. The impact of this issue on quantifying PM has remained largely uncharacterized. In this study, we incorporate in CMB the gas-particle partitioning of VE organic aerosols and hopane oxidation, which is equivalent to adopting dynamic VE source profiles. The modified version of CMB is applied to quantify primary PM contributions at a roadside and a general urban site in Hong Kong. For the roadside site, the modified CMB reports predominant PM by diesel VE, a result consistent with previous studies. For the general urban site, the apportioned gasoline contribution by the modified CMB is tripled (0.8 ± 0.5 vs. 2.7 ± 2.1 μg/m) while the diesel contribution is reduced by one third (1.7 ± 1.2 vs. 1.1 ± 1.2 μg/m), producing a gasoline-diesel split significantly different from that by traditional CMB (1:2 vs. 5:2). Our work strongly indicates that a static representation of VE source profiles in CMB modeling would create flawed PM estimation and demonstrates the necessity of considering gas-particle partitioning of organic aerosol and hopane oxidation degradation.
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http://dx.doi.org/10.1016/j.envpol.2021.118131DOI Listing
September 2021

Biomechanical assessment of two types and two different locations of subtalar arthroereisis implants for flexible flatfoot: A cadaveric study.

Clin Biomech (Bristol, Avon) 2021 Aug 30;89:105475. Epub 2021 Aug 30.

Department of Orthopedics, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, Shanghai 200040, China. Electronic address:

Background: Subtalar arthroereisis refers to the implantation of a sinus tarsi implant for the treatment of flexible flatfoot. The purpose of this study was to compare the ability to correct the flatfoot deformity and contact pressure of the posterior subtalar joint between two types of self-locking wedge implants and between two different positions for the same device in a cadaveric flatfoot model.

Methods: The flatfoot model was created in ten cadaver feet through ligament sectioning and cyclic loading. Three kinds of arthroereisis procedures were evaluated: Talar-Fit (type I self-locking wedge implant) anchored in the sinus portion of the tarsal sinus (T-sinus group), Talar-Fit in the canalis portion (T-canalis group), and HyProCure (type II) in the canalis portion (H group). Corrective ability in the sagittal and transverse planes were measured with clinometers. Contact pressure was measured with pressure-sensitive films.

Findings: T-canalis group provided more sagittal (mean difference for size 10 mm: 1.9°, P = 0.014; mean difference for size 11 mm: 3.1°, P = 0.037) and transverse (mean difference for size 8 mm: 1.8°, P = 0.049; mean difference for size 11 mm: 2.2°, P = 0.049) corrections than T-sinus group. The flattening process shifted the peak pressure of the posterior subtalar joint to the posteromedial side (P < 0.05) and arthroereisis helped the distribution of contact pressure restore uniformity (all P > 0.05).

Interpretation: A self-locking wedge implant inserted in the canalis portion of the tarsal sinus achieved better correction than an implant inserted in the sinus portion.
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http://dx.doi.org/10.1016/j.clinbiomech.2021.105475DOI Listing
August 2021

Estimation of Aromatic Secondary Organic Aerosol Using a Molecular Tracer-A Chemical Transport Model Assessment.

Environ Sci Technol 2021 10 15;55(19):12882-12892. Epub 2021 Sep 15.

Zachry Department of Civil and Environmental Engineering, Texas A&M University, College Station, Texas 77843-3136, United States.

A modified community multiscale air quality model, which can simulate the regional distributions of 2,3-dihydroxy-4-oxopentanoic acid (DHOPA), a marker species for monoaromatic secondary organic aerosol (SOA), was applied to assess the applicability of using the DHOPA to aromatic SOA mass ratio () from smog chamber experiments to estimate aromatic SOA during a three-week wintertime air quality campaign in urban Shanghai. The modeled daily DHOPA concentrations based on the chamber-derived mass yields agree well with the organic marker field measurements ( = 0.79; MFB = 0.152; and MFE = 0.440). Two-thirds of the DHOPA are from the oxidation of ARO1 (lumped less-reactive aromatic species; mostly toluene), with the rest from ARO2 (lumped more-reactive aromatic species; mostly xylenes). Modeled DHOPA is mainly in the particle phase under ambient organic aerosol (OA) loading but could exhibit significant gas-particle partitioning when a higher estimation of the DHOPA vapor pressure is used. The modeled shows a strong dependence on the OA loading when only semivolatile aromatic SOA components are included in the calculations. However, this OA dependence becomes weaker when non-volatile oligomers and dicarbonyl SOA products are considered. A constant value of ∼0.002 is determined when all aromatic SOA components are included, which is a factor of 2 smaller than the commonly applied chamber-based value of 0.004 for toluene. This model-derived value does not show much spatial variation and is not sensitive to alternative estimates of DHOPA vapor pressures and SOA yields, and thus provides an appropriate scaling factor to assess aromatic SOA from DHOPA measurements. This result helps refine the quantification of SOA attributable to monoaromatic hydrocarbons in urban environments and thereby facilitates the evaluation of control measures targeting these specific precursors.
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http://dx.doi.org/10.1021/acs.est.1c03670DOI Listing
October 2021

Factors influencing the coping abilities in clinic nursing students under public health emergency (COVID-19): a cross-sectional study.

BMC Nurs 2021 Sep 13;20(1):167. Epub 2021 Sep 13.

Nursing Department, The First Affiliated Hospital with Nanjing Medical University/Jiangsu Provice Hospital, 300 Guangzhou Road, Nanjing, Jiangsu Province, China.

Background: Public health emergencies are serious social problems, threatening people's lives, causing considerable economic losses, and related to all mankind life and health and safety. Nurses are essential in the fight against the public health emergency, corona virus disease 2019 (COVID-19). clinic nursing students are considered as backup health care providers for licensed nurses, the coping abilities and crisis management of nursing students at present deserve attention all around the world.

Methods: 2035 clinic nursing students were invited to participate in mobile phone app-based survey from Feb 6 to 20, 2020. The demographic items, psychological and behavioral responses, and the coping abilities were conducted. Multiple linear regression was used to identify the independent factors to clinic nursing students' coping abilities under COVID-19.

Results: 1992 submitted were valid. Multiple linear regression analysis showed that Confidence to overcome difficulties, Optimism, Active coping, Help seeking and Practice hospital as designated treatment unit were independently associated with the positive coping of clinic nursing students. Fear of COVID-19, Optimism, Avoidance, Help seeking and Severity of epidemic around were independently associated with the negative coping of clinic nursing students.

Conclusion: Under COVID-19, nursing students' coping level is superior to the Chinese norm, which is also affected by many factors. As the most direct backup resources of professional nurses, the way clinic nursing students respond to public health emergencies and its influencing factors deserve attention.
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http://dx.doi.org/10.1186/s12912-021-00686-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436027PMC
September 2021

Identifying Protein Complexes With Clear Module Structure Using Pairwise Constraints in Protein Interaction Networks.

Front Genet 2021 27;12:664786. Epub 2021 Aug 27.

Beijing Key Lab of Traffic Data Analysis and Mining, School of Computer and Information Technology, Beijing Jiaotong University, Beijing, China.

The protein-protein interaction (PPI) networks can be regarded as powerful platforms to elucidate the principle and mechanism of cellular organization. Uncovering protein complexes from PPI networks will lead to a better understanding of the science of biological function in cellular systems. In recent decades, numerous computational algorithms have been developed to identify protein complexes. However, the majority of them primarily concern the topological structure of PPI networks and lack of the consideration for the native organized structure among protein complexes. The PPI networks generated by high-throughput technology include a fraction of false protein interactions which make it difficult to identify protein complexes efficiently. To tackle these challenges, we propose a novel semi-supervised protein complex detection model based on non-negative matrix tri-factorization, which not only considers topological structure of a PPI network but also makes full use of available high quality known protein pairs with must-link constraints. We propose non-overlapping (NSSNMTF) and overlapping (OSSNMTF) protein complex detection algorithms to identify the significant protein complexes with clear module structures from PPI networks. In addition, the proposed two protein complex detection algorithms outperform a diverse range of state-of-the-art protein complex identification algorithms on both synthetic networks and human related PPI networks.
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http://dx.doi.org/10.3389/fgene.2021.664786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430217PMC
August 2021

Comparison of Three Interfacial Conductive Networks Formed in Carbon Black-Filled PA6/PBT Blends.

Polymers (Basel) 2021 Aug 30;13(17). Epub 2021 Aug 30.

Key Laboratory of Advanced Materials (MOE), Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.

Interfacial localization of carbon fillers in cocontinuous-structured polymer blends is well-known as a high-efficiency strategy for conductive network formation. However, a comparison with interfacial localization of carbon fillers in sea-island-structured polymer blends is lacking. Here, three types of highly efficient conductive networks formed on the basis of interfacial localization of carbon black (CB) in polyamide 6 (PA6)/poly(butylene terephthalate) (PBT) blends with different blend compositions (80/20, 50/50 and 20/80 ) were investigated and compared in terms of electrical resistivity, morphology as well as rheological and mechanical properties. The order of the electrical percolation threshold of CB in the three blends is 50/50 < 20/80 < 80/20, which can be attributed to different network structures. The rheological percolation thresholds are close to the electrical ones, confirming the formation of CB networks. The formation mechanisms for the three types of CB network structures are analyzed. All the three types of PA6/PBT-6 vol% CB composites showed improved tensile strength compared with PA6/PBT blends, being in favor for practical applications.
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http://dx.doi.org/10.3390/polym13172926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434115PMC
August 2021

Combination Usage of AdipoCount and Image-Pro Plus/ImageJ Software for Quantification of Adipocyte Sizes.

Front Endocrinol (Lausanne) 2021 4;12:642000. Epub 2021 Aug 4.

Department of Endocrine and Metabolic Diseases, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

In recent decades, the prevalence of obesity has been rising. One of the major characteristics of obesity is fat accumulation, including hyperplasia (increase in number) and hypertrophy (increase in size). After histological staining, it is critical to accurately measure the number and size of adipocytes for assessing the severity of obesity in a timely fashion. Manual measurement is accurate but time-consuming. Although commercially available adipocyte counting tools, including AdipoCount, Image-Pro Plus, and ImageJ were helpful, limitations still exist in accuracy and time consuming. In the present study, we introduced the protocol of combined usage of these tools and illustrated the process with histological staining slides from adipose tissues of lean and obese mice. We found that the adipocyte sizes quantified by the tool combination were comparable as manual measurement, whereas the combined methods were more efficient. Besides, the recognition effect of monochrome segmentation image is better than that of color segmentation image. Overall, we developed a combination method to measure adipocyte sizes accurately and efficiently, which may be helpful for experimental process in laboratory and also for clinic diagnosis.
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http://dx.doi.org/10.3389/fendo.2021.642000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371441PMC
August 2021

Circ_0027089 regulates NACC1 by targeting miR-136-5p to aggravate the development of hepatitis B virus-related hepatocellular carcinoma.

Anticancer Drugs 2021 Aug 19. Epub 2021 Aug 19.

Department of General Surgery, Lichuan People's Hospital, Lichuan Department of General Surgery, the Fourth Affiliated Hospital of Anhui Medical University, Anhui Department of Respiratory Medicine, Lichuan People's Hospital, Lichuan, China.

Hepatitis B virus (HBV) infection is the main trigger of hepatocellular carcinoma (HCC). Circular RNA plays an indispensable role in cancer development, and this study aimed to disclose the function and mechanism of circ_0027089 in HBV-related HCC. The expression levels of circ_0027089, miR-136-5p and nucleus accumbens associated protein 1 (NACC1) mRNA were measured by quantitative real-time PCR, and the protein level of NACC1 was detected by western blot. For functional analyses, cell proliferation was assessed by cell counting kit-8 assay and colony formation assay. Cell apoptosis and cell cycle were detected by flow cytometry assay, and cell apoptosis was also assessed by caspase 3/7 activity. The capacities of migration and invasion were evaluated by wound healing assay and transwell assay, respectively. The predicted relationship between miR-136-5p and circ_0027089 or NACC1 was validated by dual-luciferase reporter assay and RNA binding protein immunoprecipitation assay. Animal experiments were performed in nude mice to explore the role of circ_0027089 in vivo. Circ_0027089 expression and NACC1 expression were elevated, while miR-136-5p expression was decreased in HBV-related HCC tissues and cells. In function, circ_0027089 knockdown inhibited HepG2.2.15 and HepAD38 (tet-off) cell proliferation, migration and invasion but induced cell cycle arrest and apoptosis, while circ_0027089 overexpression played the reversed effects. For mechanism exploration, miR-136-5p was a target of circ_0027089, and miR-136-5p deficiency could reverse the role of circ_0027089 knockdown. Circ_0027089 functioned as an oncogene to promote the development of HBV-related HCC by regulating NACC1 via competitively targeting miR-136-5p.
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http://dx.doi.org/10.1097/CAD.0000000000001211DOI Listing
August 2021

Simultaneous Determination of Aerosol Inorganic and Organic Nitrogen by Thermal Evolution and Chemiluminescence Detection.

Environ Sci Technol 2021 09 16;55(17):11579-11589. Epub 2021 Aug 16.

Division of Environment and Sustainability, Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong 999077, China.

Inorganic nitrogen (IN) and organic nitrogen (ON) molecules constitute a significant part of atmospheric aerosol. Unlike IN, the total ON quantity remains largely unquantified due to a lack of a simple and direct measurement method. This analytical deficiency hinders the quantitative assessment of the various environmental and health effect impacts by aerosol ON. In this work, we developed an analyzer system that utilizes programmed thermal evolution of carbonaceous and nitrogenous aerosols and chemiluminescence detection coupled with the multivariate curve resolution data treatment to achieve simultaneous quantification of IN and ON. The system is capable of detecting IN and ON as low as 96 ng N per sample on a small filter aliquot (1 cm) without any pretreatment. This method breakthrough opens the door to quantifying an important pool of aerosol N that was analytically inaccessible in the past and holds the promise to quantifying IN and ON in other environmental samples. As a demonstration, quantification of aerosol ON at an urban site in Hong Kong, China, in samples spanning over a year reveals ON constituting a significant fraction (9-52%) of the total aerosol nitrogen and having major source origins in both secondary formation and primary emissions.
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http://dx.doi.org/10.1021/acs.est.1c04876DOI Listing
September 2021

Phase Tuning for Enhancing the Thermoelectric Performance of Solution-Synthesized CuS.

ACS Appl Mater Interfaces 2021 Aug 12;13(33):39541-39549. Epub 2021 Aug 12.

College of Materials Science and Engineering, Chongqing University, Chongqing 400044, China.

CuS, consisting of Earth-abundant and eco-friendly elements, is a promising candidate for thermoelectric energy conversion. The stoichiometric CuS has intrinsically low thermal conductivity together with meagre electrical conductivity. Herein, in order to improve the electrical properties, we develop a facile solvothermal method for synthesizing a series of CuS with tunable phase compositions. With reduction in the CuCl/NaS molar ratio in the solution precursors from 2:1 to 1.75:1, the solvothermal products transform from single-phase CuS into CuS-CuS composites, which are preserved in the hot-pressed pellets. The decreased CuCl/NaS molar ratio results in increased room-temperature Hall carrier concentration, leading to enhanced electrical conductivity. Specifically, the pellet corresponding to the 1.75:1 CuCl/NaS precursor ratio obtains a power factor of 8.04 μW cm K at 815 K, which is ∼735% of the value obtained by stoichiometric CuS. Along with the moderate thermal conductivity, this sample achieves an enhanced value of 0.87 at 815 K, representing a ∼250% increase when compared to stoichiometric CuS. This study demonstrates an effective strategy in enhancing the thermoelectric performance of solution-synthesized CuS-based materials by phase tuning.
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http://dx.doi.org/10.1021/acsami.1c13050DOI Listing
August 2021

Improved survival for young acute leukemia patients following a new donor hierarchy for allogeneic hematopoietic stem cell transplantation: A phase III randomized controlled study.

Am J Hematol 2021 Nov 22;96(11):1429-1440. Epub 2021 Aug 22.

Bone Marrow Transplantation Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

The aim of our study was to evaluate the most optimal donor for young acute leukemia (AL) patients with multiple donors available for allogeneic hematopoietic stem cell transplantation (allo-HSCT), including HLA-matched sibling donors (MSDs), HLA-matched unrelated donors (URDs), haploidentical parental donors (HPDs), and haploidentical sibling donors (HSDs). From March 2008 to December 2016, 430 AL patients ≤ 35 years of age were included in the discovery, retrospective study. Patients who received transplantation from a MSD or a HSD had better 5-year OS rates compared with patients who received transplantation from a URD or a HPD. A superior graft-versus-leukemia effect was observed for high-risk patients undergoing HSD-HSCT with a lower relapse rate (p = 0.014) and a higher disease-free survival (DFS) rate (p = 0.029) compared with those undergoing MSD-HSCT. Outcomes of high-risk patients receiving an URD or HPD were equivalent. For intermediate/standard-risk patients, either a MSD or HSD may be the front-line donor selection with comparable outcomes. HLA-matched unrelated donors were preferred over HPDs with reduced non-relapse mortality and higher overall survival (OS) and DFS rates. We further conducted an independent prospective randomized study to evaluate the survival advantage with the new donor hierarchy. Two hundred and fifty patients were randomly assigned to follow our new donor hierarchy or the traditional donor hierarchy at a 2:1 ratio. The new donor hierarchy contributed to significantly superior 2-year OS and DFS rates (OS: 76.2% vs. 67.8%, p = 0.046; DFS: 71.8% vs. 64.5%, p = 0.039).
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http://dx.doi.org/10.1002/ajh.26317DOI Listing
November 2021

Association between aspirin-induced hemoglobin decline and outcome after acute ischemic stroke in G6PD-deficient patients.

CNS Neurosci Ther 2021 10 8;27(10):1206-1213. Epub 2021 Aug 8.

Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangdong Provincial Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department and Key Discipline of Neurology, Guangzhou, China.

Aims: The risk of hemoglobin decline induced by low-dose aspirin in glucose-6-phosphate dehydrogenase (G6PD) deficiency remains unknown, and its influence on stroke outcome remains to be investigated. This study aimed to evaluate the effect of G6PD deficiency on hemoglobin level during aspirin treatment and its association with outcome after acute ischemic stroke.

Methods: In total, 279 patients (40 G6PD-deficient and 239 G6PD-normal) with acute ischemic stroke treated with aspirin 100 mg/day from a cohort study were examined. The primary safety endpoint was a hemoglobin decline ≥25 g/L or 25% from baseline within 14 days after aspirin treatment. Poor outcomes were defined as a modified Rankin Scale score ≥2 at 3 months. The χ test was used to compare stroke outcomes, and multivariate logistic regression analyses were performed to analyze the association between hemoglobin level and outcomes.

Results: The G6PD-deficient group had lower baseline hemoglobin and tended to develop comorbid pulmonary infection more frequently (p < 0.05). The proportion of patients with hemoglobin decline ≥25 g/L or 25% from baseline (15.0% vs. 3.3%; p = 0.006) and anemia (30.0% vs. 14.6%; p = 0.016) after aspirin treatment was higher in the G6PD-deficient group, which was accompanied by a more significant bilirubin increase. The rate of poor functional outcomes at 3 months after acute ischemic stroke was higher in the G6PD-deficient group (Risk ratio = 1.31 [95% confidence interval (CI) = 1.10-1.56]; p = 0.017). Confounder-adjusted analysis showed that lower hemoglobin levels (odds ratio = 0.98 [95% CI = 0.96-0.99]; adjusted p = 0.009) increased the risk of poor functional outcomes.

Conclusion: Hemoglobin decrease with bilirubin increase after aspirin treatment in patients with G6PD deficiency suggests hemolysis, which may influence stroke prognosis. The risk of hemoglobin decline should be carefully monitored in G6PD-deficient patients with ischemic stroke taking aspirin.
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http://dx.doi.org/10.1111/cns.13711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446213PMC
October 2021

Combined Radiomics Model for Prediction of Hematoma Progression and Clinical Outcome of Cerebral Contusions in Traumatic Brain Injury.

Neurocrit Care 2021 Aug 4. Epub 2021 Aug 4.

Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.

Background: Traumatic brain injury is a common and devastating injury that is the leading cause of neurological disability and death worldwide. Patients with cerebral lobe contusion received conservative treatment because of their mild manifestations, but delayed intracranial hematoma may increase and even become life-threatening. We explored the noninvasive method to predict the prognosis of progression and Glasgow Outcome Scale (GOS) by using a quantitative radiomics approach and statistical analysis.

Methods: Eighty-eight patients who were pathologically diagnosed were retrospectively studied. The radiomics method developed in this work included image segmentation, feature extraction, and feature selection. The nomograms were established based on statistical analysis and a radiomics method. We conducted a comparative study of hematoma progression and GOS between the clinical factor alone and fusion radiomics features.

Results: Nineteen clinical factors, 513 radiomics features, and 116 locational features were considered. Among clinical factors, international normalized ratio, prothrombin time, and fibrinogen were enrolled for hematoma progression. As for GOS, treatment strategy, age, Glasgow Coma Scale score, and blood platelet were associated factors. Eight features for GOS and five features for hematoma progression were filtered by using sparse representation and locality preserving projection-combined method. Four nomograms were constructed. After fusion radiomics features, area under the curve of hematoma progression prediction increased from 0.832 to 0.899, whereas GOS prediction went from 0.794 to 0.844.

Conclusions: A radiomic-based model that merges radiomics and clinical features is a noninvasive approach to predict hematoma progression and clinical outcomes of cerebral contusions in traumatic brain injury.
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http://dx.doi.org/10.1007/s12028-021-01320-2DOI Listing
August 2021

Forensic characteristics and population construction of two major minorities from southwest China revealed by a novel 37 Y-STR loci system.

R Soc Open Sci 2021 Jul 28;8(7):210447. Epub 2021 Jul 28.

Key Laboratory of Cell Engineering in Guizhou Province, Affiliated Hospital of Zunyi Medical University, Zunyi 563099, Guizhou, People's Republic of China.

Y-chromosome short tandem repeats (Y-STRs) have become important supplementary evidence in forensic science. Nowadays, the Y-chromosome STR haplotype reference database (YHRD) contains abundant Y-STR haplotype data from all over the world, while haplotype data of Guizhou Miao and Tujia are scarce. Hence, genetic polymorphisms of 37 Y-STRs were investigated in 446 unrelated males (206 Miao males and 246 Tujia males) residing in Guizhou Province. A total of 206 and 242 unique haplotypes with the highest diversity value of 0.9665 and 0.9470 were obtained. The heatmap, multidimensional scaling (MDS), the unweighted pair-group method with arithmetic means (UPGMA) tree and principal component analysis (PCA) based on the genetic distance (Rst) value within our studied populations and other 26 populations indicated that population structures follow the boundary of the continent. Guizhou Miao and Guizhou Tujia populations have intimate relationships with East Asian populations, especially the geographically close, similar history and the same language family populations.
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http://dx.doi.org/10.1098/rsos.210447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316789PMC
July 2021

A Progressive Generative Adversarial Method for Structurally Inadequate Medical Image Data Augmentation.

IEEE J Biomed Health Inform 2021 Aug 4;PP. Epub 2021 Aug 4.

The generation-based data augmentation method can overcome the challenge caused by the imbalance of medical image data to a certain extent. However, most of the current research focus on images with unified structure which are easy to learn. What is different is that ultrasound images are structurally inadequate, making it difficult for the structure to be captured by the generative network, resulting in the generated image lacks structural legitimacy. Therefore, a Progressive Generative Adversarial Method for Structurally Inadequate Medical Image Data Augmentation is proposed in this paper, including a network and a strategy. Our Progressive Texture Generative Adversarial Network alleviates the adverse effect of completely truncating the reconstruction of structure and texture during the generation process and enhances the implicit association between structure and texture. The Image Data Augmentation Strategy based on Mask-Reconstruction overcomes data imbalance from a novel perspective, maintains the legitimacy of the structure in the generated data, as well as increases the diversity of disease data interpretably. The experiments prove the effectiveness of our method on data augmentation and image reconstruction on Structurally Inadequate Medical Image both qualitatively and quantitatively. Finally, the weakly supervised segmentation of the lesion is the additional contribution of our method.
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http://dx.doi.org/10.1109/JBHI.2021.3101551DOI Listing
August 2021

Pleiotrophin Potentiates Sevoflurane Anesthesia-induced Learning Deficits in Mice.

J Mol Neurosci 2021 Aug 4. Epub 2021 Aug 4.

Department of Anesthesiology, Cangzhou Central Hospital, No. 16 Xinhua Road, Cangzhou, Hebei, 061000, China.

Postoperative cognitive dysfunction (POCD) is a major postoperative neurological complication in children and the elderly. However, the detailed mechanisms underlying this process remain unclear. This study aims to investigate the effect of pleiotrophin on sevoflurane-induced neuroinflammation and cognitive impairment. The novel object recognition test was performed to evaluate the cognitive and motor function of aged C57BL/6 (wild-type, WT) and pleiotrophin-knockout mice treated with sevoflurane. Small molecule inhibitors targeting receptor protein tyrosine phosphatase (RPTP) β/ζ, a pleiotrophin receptor, were used to ameliorate cognitive dysfunction. Sevoflurane treatment induced cognitive dysfunction and motor impairment in aged WT mice. Sevoflurane anesthesia induced the upregulation of certain inflammatory cytokines. Pleiotrophin knockout ameliorated the sevoflurane-induced cognitive dysfunction and motor impairment in vivo. Treatment with small molecule inhibitors targeting RPTP β/ζ inhibited sevoflurane-induced neuroinflammation. In summary, pleiotrophin was shown to potentiate sevoflurane anesthesia-induced cognitive dysfunction and learning deficits in mice.
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http://dx.doi.org/10.1007/s12031-021-01885-9DOI Listing
August 2021
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