Publications by authors named "Jian Wu"

2,663 Publications

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Generating an oilseed rape mutant with non-abscising floral organs using CRISPR/Cas9 technology.

Plant Physiol 2022 Aug 11. Epub 2022 Aug 11.

Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China.

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http://dx.doi.org/10.1093/plphys/kiac364DOI Listing
August 2022

Cytochrome bc1 Complex: Potential Breach to Improve the Activity of Phenazines on .

J Agric Food Chem 2022 Aug 10. Epub 2022 Aug 10.

Institute of Plant Protection and Agro Product Safety, Anhui Academy of Agricultural Sciences, Hefei 230001, China.

The effects of the natural pesticides, phenazines, were reported to be limited by some tolerant metabolism processes within . Our previous studies suggested that the functional cytochrome bc1 complex, the indispensable component of the respiration chain, might participate in tolerating phenazines in . In this study, the cytochrome bc1 mutants of pv. () and pv. (), which exhibit different tolerance abilities to phenazines, were constructed, and the cytochrome bc1 complex was proven to partake a critical and conserved role in tolerating phenazines in . In addition, results of the cytochrome mutants suggested the different functions of the various cytochrome proteins in and that the electron channeled by the cytochrome bc1 complex to cytochrome C4 is the key to reveal the tolerance mechanism. In conclusion, the study of the cytochrome bc1 complex provides a potential strategy to improve the activity of phenazines against .
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http://dx.doi.org/10.1021/acs.jafc.2c03317DOI Listing
August 2022

Multi-slice compressed sensing MRI reconstruction based on deep fusion connection network.

Magn Reson Imaging 2022 Aug 6. Epub 2022 Aug 6.

Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen 361005, China. Electronic address:

Recently, magnetic resonance imaging (MRI) reconstruction based on deep learning has become popular. Nevertheless, reconstruction of highly undersampled MR images is still challenging due to severe aliasing effects. In this study we built a deep fusion connection network (DFCN) to efficiently utilize the correlation information between adjacent slices. The proposed method was evaluated with online public IXI dataset and Calgary-Campinas-359 dataset. The results show that DFCN can generate the best reconstruction images in de-aliasing and restoring tissue structure compared with several state-of-the-art methods. The mean value of the peak signal-to-noise ratio could reach 34.16 dB, the mean value of the structural similarity is 0.9626, and the mean value of the normalized mean square error is 0.1144 on T-weighted brain data of IXI dataset under 10× acceleration. Additionally, the mean value of the peak signal-to-noise ratio could reach 30.17 dB, the mean value of the structural similarity is 0.9259, and the mean value of the normalized mean square error is 0.1294 on T-weighted brain data of Calgary-Campinas-359 dataset under 10× acceleration. With the correlation information between adjacent slices as prior knowledge, our method can dramatically eliminate aliasing effects and enhance the reconstruction quality of undersampled MR images.
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http://dx.doi.org/10.1016/j.mri.2022.08.007DOI Listing
August 2022

The diagnostic utility of IL-10, IL-17, and PCT in patients with sepsis infection.

Front Public Health 2022 22;10:923457. Epub 2022 Jul 22.

Department of Laboratory Medicine, Shanghai Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.

Objective: The purpose of this study is to determine the diagnostic value and net clinical benefit of interleukin-10 (IL-10), interleukin-17 (IL-17), procalcitonin (PCT), and combination tests in patients with sepsis, which will serve as a standard for sepsis early detection.

Patients And Methods: An investigation of 84 sepsis patients and 81 patients with local inflammatory diseases admitted to the ICU of Tongji University Hospital in 2021. In addition to comparing inter-group variability, indicators relevant to sepsis diagnosis and therapy were screened.

Results: LASSO regression was used to examine PCT, WBC, CRP, IL-10, IFN-, IL-12, and IL-17. Multivariate logistic regression linked IL-10, IL-17, and PCT to sepsis risk. The AUC values of IL-10, IL-17, PCT, and the combination of the three tests were much higher than those of standard laboratory infection indicators. The combined AUC was greater than the sum of IL-10, IL-17, and PCT ( < 0.05). A clinical decision curve analysis of IL-10, IL-17, PCT, and the three combined tests found that the three combined tests outperformed the individual tests in terms of total clinical benefit rate. To predict the risk of sepsis using IL-10, IL-17, and PCT had an AUC of 0.951, and the model's predicted probability was well matched. An examination of the nomogram model's clinical value demonstrated a considerable net therapeutic benefit between 3 and 87%.

Conclusion: The IL-10, IL-17, and PCT tests all have a high diagnostic value for patients with sepsis, and the combination of the three tests outperforms the individual tests in terms of diagnostic performance, while the combined tests have a higher overall clinical benefit rate.
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http://dx.doi.org/10.3389/fpubh.2022.923457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355284PMC
August 2022

Comparison of the Differential Diagnostic Performance of Intravoxel Incoherent Motion Imaging and Diffusion Kurtosis Imaging in Malignant and Benign Thyroid Nodules.

Front Oncol 2022 22;12:895972. Epub 2022 Jul 22.

Department of Radiology, Cancer Hospital, Chongqing University, Chongqing, China.

Objective: This study aimed to compare the diagnostic capacity between IVIM and DKI in differentiating malignant from benign thyroid nodules.

Material And Methods: This study is based on magnetic resonance imaging data of the thyroid with histopathology as the reference standard. Spearman analysis was used to assess the relationship of IVIM-derived parameters D, f, D* and the DKI-derived parameters D and K. The parameters of IVIM and DKI were compared between the malignant and benign groups. Binary logistic regression analysis was performed to establish the diagnostic model, and receiver operating characteristic (ROC) curve analysis was subsequently performed. The DeLong test was used to compare the diagnostic effectiveness of different prediction models. Spearman analysis was used to assess the relationship of Ki-67 expression and parameters of IVIM and DKI.

Results: Among the 93 nodules, 46 nodules were malignant, and 47 nodules were benign. The D of DKI-derived parameter was related to the D (P < 0.001, r = 0.863) of IVIM-derived parameter. The K of DKI-derived parameter was related to the D (P < 0.001, r = -0.831) of IVIM-derived parameters. The malignant group had a significantly lower D value (P < 0.001) and f value (P = 0.013) than the benign group. The malignant group had significantly higher K and lower D values (all P < 0.001). The D+f had an area under the curve (AUC) of 0.951. The D+K had an AUC of 0.943. The D+f+D+K had an AUC of 0.954. The DeLong test showed no statistical significance among there prediction models. The D (P = 0.007) of IVIM-derived parameters and D (P = 0.045) of DKI-derived parameter were correlated to the Ki-67 expression.

Conclusions: IVIM and DKI were alternative for each other in in differentiating malignant from benign thyroid nodules.
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http://dx.doi.org/10.3389/fonc.2022.895972DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354485PMC
July 2022

A briefly overview of the research progress for the abscisic acid analogues.

Front Chem 2022 22;10:967404. Epub 2022 Jul 22.

State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang, China.

Abscisic acid (ABA) is an important plant endogenous hormone that participates in the regulation of various physiological processes in plants, including the occurrence and development of somatic embryos, seeddevelopment and dormancy. ABA is called "plant stress resistance factor", while with the limitation of the rapid metabolic inactivation and photoisomerization inactivation of ABA for its large-scale use. Understanding the function and role of ABA in plants is of great significance to promote its application. For decades, scientists have conducted in-depth research on its mechanism of action and signaling pathways, a series of progress were achieved, and hundreds of ABA analogues (similar in structure or function) have been synthesized to develop highly active plant growth regulators and tools to elucidate ABA perception. In this review, we summarize a variety of ABA analogues, especially the ABA receptor analogues, and explore the mechanisms of ABA action and catabolism, which will facilitate the development of novel ABA analogues with high biological activities.
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http://dx.doi.org/10.3389/fchem.2022.967404DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355028PMC
July 2022

Genome-wide association study of chronic spontaneous urticaria reveals genetic overlap with autoimmune diseases, not atopic diseases.

J Invest Dermatol 2022 Aug 3. Epub 2022 Aug 3.

Department of Dermatology, The Second Hospital of Jilin University, Changchun, China.

Although chronic spontaneous urticaria (CSU) is a common disease, genome-wide association studies (GWASs) of CSU are lacking. We aimed to identify susceptibility single-nucleotide polymorphism (SNPs) by performing to our knowledge previously unreported GWAS in Chinese Han adults with CSU. The discovery cohort included 430 CSU cases and 482 healthy controls. The GWAS findings were validated in 800 CSU cases and 900 healthy controls. Genetic, functional enrichment and bioinformatic analyses of genome-wide significant SNPs were performed to assess association between CSU and autoimmunity or atopy. Five genome-wide significant SNPs were identified: rs434124/LILRA3, rs61986182/IGHG1/2, rs73075571/TDGF1, rs9378141/HLA-G and rs3789612/PTPN22. The first 4 SNPs were in linkage disequilibrium with autoimmune-related diseases-associated SNPs and were cis expression quantitative trait loci in immune cells. The 5 SNPs-annotated genes were significantly enriched in immune processes. Higher polygenic risk scores, allele frequencies of rs3789612*T, rs9378141*C and rs73075571*G were significantly associated with autoimmune-related CSU phenotypes including positive anti-thyroglobulin IgG, positive anti-FcεRIα IgG, total IgE < 40 IU/mL and positive anti-thyroid peroxidase IgG, not with atopic or allergic sensitized CSU phenotypes. This GWAS of CSU identifies 5 risk loci and reveals that CSU shares genetic overlap with autoimmune diseases and that genetic factors predisposing to CSU mainly manifest through associations with autoimmune traits.
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http://dx.doi.org/10.1016/j.jid.2022.07.012DOI Listing
August 2022

Fumigant toxicity and behavioral inhibition of garlic against red imported fire ants (Solenopsis invicta).

Environ Sci Pollut Res Int 2022 Aug 4. Epub 2022 Aug 4.

Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education, South China Agricultural University, Guangzhou, 510642, China.

In order to control the Solenopsis invicta (S. invicta) in a safe and ecofriendly manner, this study evaluated the fumigating activity of garlic and its effect on the behavior of S. invicta. The volatile compounds and chemical constituents from garlic were collected by solid phase micro-extraction and identified by gas chromatography-mass spectrometry (GC-MS). The results showed that garlic contains 21 compounds, and that diallyl disulfide (46.51%) and (E)-1-Allyl-2-(prop-1-en-1-yl) disulfane (34.68%) were the main constituents in garlic. In addition, garlic showed strong fumigant activity and behavioral inhibition against workers. The LC values of diallyl disulfide and methyl allyl disulfide against S. invicta at 12 h were 0.05 and 0.07 μg/L, respectively. The grasping and walking abilities of ants were significantly suppressed. Moreover, minor workers were more susceptible than major workers. Thus, S. invicta can be controlled with garlic and some of its compounds as an eco-friendly approach. Results from the study could provide valuable advances for future fumigant development and possible utility in fumigant use.
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http://dx.doi.org/10.1007/s11356-022-22091-zDOI Listing
August 2022

The Sclerotinia sclerotiorum-inducible promoter pBnGH17D7 in Brassica napus: isolation, characterization and application in host-induced gene silencing.

J Exp Bot 2022 Aug 5. Epub 2022 Aug 5.

Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China.

Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum, is among the most devastating diseases in Brassica napus worldwide. Conventional breeding for SSR resistance in Brassica species is challenging due to the limited availability of resistant germplasm. Therefore, genetic engineering is an attractive means for developing SSR-resistant Brassica crops. Compared with constitutive promoter, S. sclerotiorum-inducible promoter would avoid ectopic expression of defense genes that may cause plant growth deficits. In this study, we generated a S. sclerotiorum-inducible promoter. pBnGH17D7, from the promoter of B. napus glycosyl hydrolase 17 gene (pBnGH17). Specifically, 5'-deletion and promoter activity analyses in transgenic A. thaliana plants defined a 189-bp region of pBnGH17, that was indispensable for S. sclerotiorum-induced response. Compared with pBnGH17, pBnGH17D7 showed a similar response upon S. sclerotiorum infection, but lower activity in plant tissues in the absence of S. sclerotiorum infection. Moreover, we revealed that the transcription factor BnTGA7 directly binds to the TGACG motif in pBnGH17D7 to activate BnGH17. Ultimately, pBnGH17D7 was exploited for engineering Sclerotinia-resistant B. napus via host-induced gene silencing. It induces high expression of small interfering RNAs against S. sclerotiorum pathogenic-factor gene specifically during infection, leading to increased resistance.
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http://dx.doi.org/10.1093/jxb/erac328DOI Listing
August 2022

The origins, isolation, and biological characterization of rodent malaria parasites.

Parasitol Int 2022 Aug 1;91:102636. Epub 2022 Aug 1.

Malaria Functional Genomics Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, USA. Electronic address:

Rodent malaria parasites have been widely used in all aspects of malaria research to study parasite development within rodent and insect hosts, drug resistance, disease pathogenesis, host immune response, and vaccine efficacy. Rodent malaria parasites were isolated from African thicket rats and initially characterized by scientists at the University of Edinburgh, UK, particularly by Drs. Richard Carter, David Walliker, and colleagues. Through their efforts and elegant work, many rodent malaria parasite species, subspecies, and strains are now available. Because of the ease of maintaining these parasites in laboratory mice, genetic crosses can be performed to map the parasite and host genes contributing to parasite growth and disease severity. Recombinant DNA technologies are now available to manipulate the parasite genomes and to study gene functions efficiently. In this chapter, we provide a brief history of the isolation and species identification of rodent malaria parasites. We also discuss some recent studies to further characterize the different developing stages of the parasites including parasite genomes and chromosomes. Although there are differences between rodent and human malaria parasite infections, the knowledge gained from studies of rodent malaria parasites has contributed greatly to our understanding of and the fight against human malaria.
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http://dx.doi.org/10.1016/j.parint.2022.102636DOI Listing
August 2022

Genetic mapping of determinants in drug resistance, virulence, disease susceptibility, and interaction of host-rodent malaria parasites.

Parasitol Int 2022 Aug 1;91:102637. Epub 2022 Aug 1.

Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand. Electronic address:

Genetic mapping has been widely employed to search for genes linked to phenotypes/traits of interest. Because of the ease of maintaining rodent malaria parasites in laboratory mice, many genetic crosses of rodent malaria parasites have been performed to map the parasite genes contributing to malaria parasite development, drug resistance, host immune response, and disease pathogenesis. Drs. Richard Carter, David Walliker, and colleagues at the University of Edinburgh, UK, were the pioneers in developing the systems for genetic mapping of malaria parasite traits, including characterization of genetic markers to follow the inheritance and recombination of parasite chromosomes and performing the first genetic cross using rodent malaria parasites. Additionally, many genetic crosses of inbred mice have been performed to link mouse chromosomal loci to the susceptibility to malaria parasite infections. In this chapter, we review and discuss past and recent advances in genetic marker development, performing genetic crosses, and genetic mapping of both parasite and host genes. Genetic mappings using models of rodent malaria parasites and inbred mice have contributed greatly to our understanding of malaria, including parasite development within their hosts, mechanism of drug resistance, and host-parasite interaction.
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http://dx.doi.org/10.1016/j.parint.2022.102637DOI Listing
August 2022

Microplastics in personal care products: Exploring public intention of usage by extending the theory of planned behaviour.

Sci Total Environ 2022 Aug 1:157782. Epub 2022 Aug 1.

Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China; Institute of Eco-Chongming (IEC), Shanghai 200062, China; Yangtze Delta Estuarine Wetland Ecosystem Observation and Research Station, Ministry of Education, Shanghai Science and Technology Committee, Shanghai 200062, China. Electronic address:

Microplastics, artificial plastic particles with a particle size of <5 mm, have attracted considerable attention due to their potential negative impacts on the social economy, ecological environment, and human health. An important direct source of microplastics (i.e., microbeads in cosmetics) is scrub particles from personal care products, such as cosmetics and toothpaste. Therefore, it is necessary to understand consumers' perceptions and behaviours regarding these products, which can help reduce the emission of microplastics at the source. The purpose of this study is to quantitatively analyse the impact factors and interaction mechanisms of the public behavioural intention of reducing the use of personal care and cosmetic products containing microplastics through the expanded theory of planned behaviour (TPB) model. We conducted random face-to-face interviews with 496 respondents in Shanghai, China. The results show that (1) attitude has the most powerful positive influence on behavioural intention, followed by perceived behavioural control and environmental concern, whereas there is no significant direct influence of subjective norms, environmental education, and behavioural experience; (2) subjective norms have an indirect influence on behavioural intention through attitude and perceived behavioural control; and (3) environmental education and behavioural experience both have an indirect impact on behavioural intention through attitude. Practical and effective policy implications are proposed for the government to reduce microplastic pollution based on the results of this article.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157782DOI Listing
August 2022

GPM6A expression is suppressed in hepatocellular carcinoma through miRNA-96 production.

Lab Invest 2022 Aug 2. Epub 2022 Aug 2.

Dept. of Medical Microbiology & Parasitology, MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology, School of Basic Medical Sciences, Fudan University, Shanghai, China.

GPM6A is a glycoprotein in endothelial cells, and its biological function in the development of hepatocellular carcinoma (HCC) is unknown. Through Affymetrix gene expression microarray and bioinformatic analysis, very low GPM6A expression was found in HCC tissue. The present study aims to explore the function and regulatory mechanism of GPM6A in HCC development and progression. Levels of GPM6A expression in HCC specimens from different disorders and various hepatoma cell lines were determined, and its role on cell proliferation was evaluated in hepatoma cells stably overexpressing GPM6A. Modulation of a specific microRNA (miRNA) on its expression and function was evaluated with miRNA mimetic transfection. Herein, it is reported that much lower GPM6A levels were found in HCC tissues than pericancerous liver tissues and correlated to a poor prognosis. GPM6A overexpression inhibited cell proliferation, suppressed colony formation, migration and invasion in two hepatoma cell types. Available evidence does not support that genetic and epigenetic dysregulation contributes significantly to GPM6A inactivation in HCC. Additional findings demonstrated that miR-96-5p acted directly on the 3'-UTR of the GPM6A gene and significantly decreased its mRNA and protein levels. MiR-96-5p transfection promoted proliferation, migration and invasion of SMMC-7721 and MHCC-97H hepatoma cells; whereas the function of oncogenic microRNA-96 was significantly inhibited in GPM6A-overexpressed hepatoma cells. In conclusion, GPM6A expression in HCC is commonly suppressed regardless its base disease types, and its low expression in HCC tissues is most likely attributed to upregulated miR-96-5p. GPM6A may function as a valuable biomarker for HCC progression and prognosis.
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http://dx.doi.org/10.1038/s41374-022-00818-3DOI Listing
August 2022

Effect of refractive status on retinal nerve fiber layer thickness in Chinese Population.

Graefes Arch Clin Exp Ophthalmol 2022 Aug 1. Epub 2022 Aug 1.

Dongcheng District, Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Laboratory, No. 1 Dong Jiao Min Xiang Street, Beijing, People's Republic of China, 100730.

Purpose: To evaluate the relationship between retinal nerve fiber layer (RNFL) thickness and other related parameters measured by spectral-domain optical coherence tomography and the refractive error of eyes.

Methods: A total of 5394 subjects were enrolled in this population-based cohort study, who were divided into three groups by refractive state after they underwent a standardized ophthalmic examination: emmetropia (the absolute value should range from 0 to 0.5 D), low-moderate myopia (the absolute value of myopic error should range from 0.5 to 6 D), and high myopia (the absolute value of myopic error should be over than 6 D). R 3.6.1 software was adopted for statistical analysis.

Results: Two thousand five hundred fifty-two subjects (4548 eyes) were collected in this study, with an average age of 53.14 ± 10.64 years. There were significant differences among groups in average central corneal curvature, spherical equivalent, and axial length (P < 0.001). The measurements of average retinal nerve fiber layer (RNFL) were 113.95 ± 10.62 μm, 112.97 ± 11.59 μm, and 101.88 ± 15.67 μm, respectively, in the emmetropia, low-moderate, and high myopia groups (P < 0.001). Meanwhile, there was a decreasing trend of cup area, cup volume, disc area, and rim area in the high myopia group compared with the emmetropia group (P < 0.001).

Conclusion: The measurements of RNFL thickness vary greatly with refractive error, and this study indicated that it is of great significance for the accurate diagnosis of glaucoma to establish an individualized RNFL thickness database.
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http://dx.doi.org/10.1007/s00417-022-05753-zDOI Listing
August 2022

Ascendancy of unfolded protein response over glioblastoma: estimating progression, prognosis and survival.

Biotechnol Genet Eng Rev 2022 Jul 29:1-23. Epub 2022 Jul 29.

f Department of Biotechnology Engineering and Food Technology, Chandigarh University, Mohali, 140413, India.

Glioblastoma (GBM) is presented with a poor prognosis. The endoplasmic reticulum stress (ERS) has been implicated as a major contributor to disease progression and chemoresistance in GBM. Triggering ERS by chemical agents or genetic modulations is identified as some of the reasons for regulating gene expression and the pathogenesis of GBM. ERS initiates unfolded protein response (UPR), an integrated system useful in restoring homeostasis or inducing apoptosis. Modulation of UPR might have positive outcomes in GBM treatment as UPR inducers have been shown to alter cell survival and migration. In the current review, we have utilized GSE7806, a publicly available dataset from Gene Expression Omnibus (GEO), to evaluate the genes expressed during 6.5 hr and 18 hr, which can be comparable to the early and late-onset of the disease. Subsequently, we have elucidated the prognosis and survival information whilst the expression of these genes in the GBM was noted in previous studies. This is the first of its kind review summarizing the most recent gene information correlating UPR and GBM.
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http://dx.doi.org/10.1080/02648725.2022.2106002DOI Listing
July 2022

Knockdown of polypyrimidine tract binding protein facilitates motor function recovery after spinal cord injury.

Neural Regen Res 2023 Feb;18(2):396-403

Key Laboratory of Neuroregeneration of Jiangsu Province and the Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University; Center for Basic Medical Research, Medical School of Nantong University; Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.

After spinal cord injury (SCI), a fibroblast- and microglia-mediated fibrotic scar is formed in the lesion core, and a glial scar is formed around the fibrotic scar as a result of the activation and proliferation of astrocytes. Simultaneously, a large number of neurons are lost in the injured area. Regulating the dense glial scar and replenishing neurons in the injured area are essential for SCI repair. Polypyrimidine tract binding protein (PTB), known as an RNA-binding protein, plays a key role in neurogenesis. Here, we utilized short hairpin RNAs (shRNAs) and antisense oligonucleotides (ASOs) to knock down PTB expression. We found that reactive spinal astrocytes from mice were directly reprogrammed into motoneuron-like cells by PTB downregulation in vitro. In a mouse model of compression-induced SCI, adeno-associated viral shRNA-mediated PTB knockdown replenished motoneuron-like cells around the injured area. Basso Mouse Scale scores and forced swim, inclined plate, cold allodynia, and hot plate tests showed that PTB knockdown promoted motor function recovery in mice but did not improve sensory perception after SCI. Furthermore, ASO-mediated PTB knockdown improved motor function restoration by not only replenishing motoneuron-like cells around the injured area but also by modestly reducing the density of the glial scar without disrupting its overall structure. Together, these findings suggest that PTB knockdown may be a promising therapeutic strategy to promote motor function recovery during spinal cord repair.
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http://dx.doi.org/10.4103/1673-5374.346463DOI Listing
February 2023

Large-Scale Customized Production Scheduling of Multiagent-Based Medical 3D Printing.

Comput Intell Neurosci 2022 18;2022:6557137. Epub 2022 Jul 18.

Department of Neurosurgery, Third Affiliated Hospital, Naval Medical University, Shanghai 200438, China.

Three-dimensional (3D) printing, also known as additive manufacturing, has unique advantages over traditional manufacturing technologies; thus, it has attracted widespread attention in the medical field. Especially in the context of the frequent occurrence of major public health events, where the medical industry's demand for large-scale and customized production is increasing, traditional 3D printing production scheduling methods take a long time to handle large-scale customized medical 3D printing (M-3DP) production and have weak intelligent collaboration ability in the face of job-to-device matching under multimaterial printing. Given the problem caused by M-3DP large-scale customized production scheduling, an intelligent collaborative scheduling multiagent-based method is proposed in this study. First, a multiagent-based optimization model is established. On this basis, an improved genetic algorithm embedded with the product mix strategy and the intelligent matching mechanism is designed to optimize the completion time and load balance between devices. Finally, the effectiveness of the proposed method is evaluated using numerical simulation. The simulation results indicated that compared with the simple genetic algorithm, particle swarm optimization, and snake optimizer, the improved genetic algorithm could better reduce the M-3DP mass customization production scheduling time, optimize the load balance between devices, and promote the "intelligent manufacturing" process of M-3DP mass customization.
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http://dx.doi.org/10.1155/2022/6557137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313918PMC
July 2022

Decreased vitamin D-binding protein level portends poor outcome in acute-on-chronic liver failure caused by hepatitis B virus.

Clin Mol Hepatol 2022 Jul 28. Epub 2022 Jul 28.

Jinan Microecological Biomedicine Shandong Laboratory, 250117 Jinan, China.

Background/aims: Acute-on-chronic liver failure (ACLF) is a catastrophic illness with high short-term mortality. A limited number of studies have investigated the prognostic value of vitamin D-binding protein (VDBP) for hepatitis B virus (HBV)-related ACLF (HBV-ACLF) and have resulted in conflicting results.

Methods: Two prospective HBV-ACLF cohorts (n=287 and n=119, respectively) were enrolled to assess and validate the prognostic performance of VDBP for HBV-ACLF.

Results: VDBP levels in the non-survivors were significantly lower than in the survivors (P<0.001). Multivariate Cox regression demonstrated that VDBP was an independent prognostic factor for HBV-ACLF. The VDBP level at admission gradually decreased as the number of failed organs increased (P<0.001), and it was closely related to coagulation failure. The areas under the receiver operating characteristic curve (AUCs) of the Child-Pugh-VDBP and chronic liver failure-sequential organ failure assessment (CLIF-SOFA)-VDBP scores were significantly higher than those of Child-Pugh (P<0.001) and CLIF-SOFA (P=0.0013). The AUCs of model for end-stage liver disease (MELD)-VDBP were significantly higher than those of MELD (P= 0.0384) only in the case of cirrhotic HBV-ACLF patients. Similar results were validated using an external multicenter HBV-ACLF cohort. By longitudinal observation, the VDBP levels gradually increased in survivors (P=0.026) and gradually decreased in non-survivors (P<0.001). Additionally, the VDBP levels were found to be significantly decreased in the deterioration group (P=0.012) and tended to decrease in the fluctuation group (P=0.055). In contrast, they showed a significant increase in the improvement group (P=0.036).

Conclusions: The VDBP was a promising prognostic biomarker for HBV-ACLF. Sequential measurement of circulating VDBP shows value for the monitoring of ACLF progression.
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http://dx.doi.org/10.3350/cmh.2022.0121DOI Listing
July 2022

T-Cell Receptor β Chain and B-Cell Receptor Repertoires in Chronic Hepatitis B Patients with Coexisting HBsAg and Anti-HBs.

Pathogens 2022 Jun 26;11(7). Epub 2022 Jun 26.

Department of Infectious Diseases, Peking University First Hospital, Beijing 100034, China.

Antibodies in response to antigens are related to the immune repertoire of T- and B-cell receptors. However, some patients with chronic hepatitis B (CHB) have coexisting HBsAg and anti-HBsAg antibodies (anti-HBs) that cannot neutralize HBV. We attempted to investigate the repertoires that produce this response in CHB patients. The T-cell receptor β chain (TRB) and B-cell receptor (BCR) repertoires of peripheral blood genomic DNA were analyzed using MiXCR. T-cell receptor (TCR) cluster analysis was carried out by clusTCR, and motifs prediction was selected by Multiple Em for Motif Elicitation (MEME). A total of 76 subjects were enrolled, including 26 HBsAg and anti-HBs coexisting patients with CHB (DP group), 25 anti-HBs single-positive healthy people (SP group), and 25 CHB patients (CHB group). The clone length of BCR in 39, 90 was significantly different among these groups ( = 0.005, 0.036). The motif "CASSLG" in the DP group was significantly higher than SP and CHB groups and may relate to coexistence, and the motif "GAGPLT" was only shown in the SP group and may relate to anti-HB expression. These provide important insights into vaccine development and CHB treatment.
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http://dx.doi.org/10.3390/pathogens11070727DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318409PMC
June 2022

Recent Progress in Flexible Pressure Sensor Arrays.

Nanomaterials (Basel) 2022 Jul 20;12(14). Epub 2022 Jul 20.

National Key Laboratory of Science and Technology on Advanced Composites in Special Environments, Harbin Institute of Technology, Harbin 150090, China.

Flexible pressure sensors that can maintain their pressure sensing ability with arbitrary deformation play an essential role in a wide range of applications, such as aerospace, prosthetics, robotics, healthcare, human-machine interfaces, and electronic skin. Flexible pressure sensors with diverse conversion principles and structural designs have been extensively studied. At present, with the development of 5G and the Internet of Things, there is a huge demand for flexible pressure sensor arrays with high resolution and sensitivity. Herein, we present a brief description of the present flexible pressure sensor arrays with different transduction mechanisms from design to fabrication. Next, we discuss the latest progress of flexible pressure sensor arrays for applications in human-machine interfaces, healthcare, and aerospace. These arrays can monitor the spatial pressure and map the trajectory with high resolution and rapid response beyond human perception. Finally, the outlook of the future and the existing problems of pressure sensor arrays are presented.
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http://dx.doi.org/10.3390/nano12142495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319019PMC
July 2022

Influencing Factors Identification and Prediction of Noise Annoyance-A Case Study on Substation Noise.

Int J Environ Res Public Health 2022 Jul 9;19(14). Epub 2022 Jul 9.

State Grid Shaanxi Electric Power Research Institute, Xi'an 710054, China.

Noise-induced annoyance is one person's individual adverse reaction to noise. Noise annoyance is an important basis for determining the acceptability of environmental noise exposure and for formulating environmental noise standards. It is influenced by both acoustic and non-acoustic factors. To identify non-acoustic factors significantly influencing noise annoyance, 40 noise samples with a loudness level of 60-90 phon from 500-1000 kV substations were selected in this study. A total of 246 subjects were recruited randomly. Using the assessment scale of noise annoyance specified by ISO 15666-2021, listening tests were conducted. Meanwhile, basic information and noise sensitivity of each subject were obtained through a questionnaire and the Weinstein's noise sensitivity scale. Based on the five non-acoustic indices which were identified in this study and had a significant influence on noise annoyance, a prediction model of annoyance from substation noise was proposed by a stepwise regression. Results showed that the influence weight of acoustic indices in the model accounted for 80% in which the equivalent continuous A-weighted sound pressure level and the sound pressure level above 1/1 octave band of 125 Hz were 65% and 15%, respectively. The influence weight of non-acoustic indices entering the model was 20% in which age, education level, noise sensitivity, income, and noisy degree in the workplace were 8%, 2%, 4%, 4%, and 2%, respectively. The result of this study can provide a basis for factors identification and prediction of noise annoyance.
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http://dx.doi.org/10.3390/ijerph19148394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315821PMC
July 2022

[Effect of electroacupuncture on gastrointestinal function of patients after laparoscopic cholecystectomy under general anesthesia].

Zhen Ci Yan Jiu 2022 Jul;47(7):640-3

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China.

Objective: To observe the effects of electroacupuncture at bilateral Zusanli(ST36), Shangjuxu(ST37), and Sanyinjiao (SP6) acupoints on gastrointestinal function after laparoscopic cholecystectomy under general anesthesia.

Methods: A total of 150 patients(American Society of Anesthesiologists[ASA] grades Ⅰ and Ⅱ) undergoing laparoscopic cholecystectomy in the Department of Hepatobiliary Surgery of the First Affiliated Hospital of Guangzhou University of Chinese Medicine were randomly assigned into three groups: electroacupuncture group (=50), simple acupuncture group (=50), and control group (=50). Patients in the control group received routine treatment;on the basis of routine treatment, patients in the simple acupuncture group were acupunctured at bilateral ST36, ST37 and SP36;patients in the electroacupuncture group were treated with electroacupuncture at bilateral ST36, ST37 and SP36(50 Hz,5 mA). In both simple acupuncture group and electroacupuncture group, the corresponding treatments were conducted 1 h after surgery, Patients as well as in the morning (8:00-10:00) and afternoon (14:00-16:00) on the first day after surgery, 30 min each time. The time of bowel sound recovery, the time to the first postope-rative exhaust and defecation, the time of postoperative fluid diet recovery, abdominal pain score, and gastrointestinal reaction score were recorded and analyzed.

Results: Compared with the control group, the electroacupuncture group and the simple acupuncture group showed shortened time of bowel sound recovery, shortened time to the first postoperative exhaust and defecation, and shortened time of postoperative liquid diet recovery (<0.01), as well as decreased postoperative abdominal pain score and gastrointestinal reaction score (<0.01). Furthermore, the time of bowel sound recovery, the time to the first postoperative exhaust and defecation, and the time of postoperative liquid diet recovery were shortened in the electroacupuncture group compared with those in the simple acupuncture group (<0.01). The total effective rate of electroacupuncture group was 94.0% (47/50), the total effective rate of simple acupuncture group was 88.0% (44/50), significantly higher than 78.0% (39/50) in the control group (<0.05).

Conclusion: Both electroacupuncture and simple acupuncture can promote the recovery of gastrointestinal function in patients after laparoscopic cholecystectomy under general anesthesia, and electroacupuncture is superior to simple acupuncture,but they have no significant difference in alleviating postoperative abdominal pain and gastrointestinal reactions.
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http://dx.doi.org/10.13702/j.1000-0607.20210711DOI Listing
July 2022

PPAR agonists attenuate lenalidomide's anti-myeloma activity in vitro and in vivo.

Cancer Lett 2022 Jul 22;545:215832. Epub 2022 Jul 22.

Division of Hematologic Malignancies and Cellular Therapy, Department of Medicine, Duke University Medical Center, Durham, NC, USA. Electronic address:

Many patients with multiple myeloma (MM) have comorbidities and are treated with PPAR agonists. Immunomodulatory agents (IMiDs) are the cornerstones for MM therapy. Currently, little is known about how co-administration of PPAR agonists impacts lenalidomide treatment in patients with MM. Here, we determined the effects of PPAR agonists on anti-myeloma activities of lenalidomide in vitro and in a myeloma xenograft mouse model. Genetic overexpression and CRISPR/cas9 knockout experiments were performed to determine the role of CRBN in the PPAR-mediated pathway. A retrospective cohort study was performed to determine the correlation of PPAR expression with the outcomes of patients with MM. PPAR agonists down-regulated CRBN expression and reduced the anti-myeloma efficacy of lenalidomide in vitro and in vivo. Co-treatment with PPAR antagonists increased CRBN expression and improved sensitivity to lenalidomide. PPAR expression was higher in bone marrow cells of patients with newly diagnosed MM than in normal control bone marrow samples. High PPAR expression was correlated with poor clinical outcomes. Our study provides the first evidence that PPARs transcriptionally regulate CRBN and that drug-drug interactions between PPAR agonists and IMiDs may impact myeloma treatment outcomes.
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http://dx.doi.org/10.1016/j.canlet.2022.215832DOI Listing
July 2022

Lattice Boltzmann modeling of two-phase electrohydrodynamic flows under unipolar charge injection.

Phys Rev E 2022 Jun;105(6-2):065304

School of Energy Science and Engineering, Harbin Institute of Technology, Harbin 150 001, People's Republic of China and Key Laboratory of Aerospace Thermophysics, Harbin Institute of Technology, Harbin 150 001, People's Republic of China.

In this work, a two-dimensional droplet confined between two parallel electrodes under the combined effects of a nonuniform electric field and unipolar charge injection is numerically investigated using the lattice Boltzmann method (LBM). Under the non-Ohmic regime, the interfacial tension and electric forces at the droplet surface cooperate with the volumetric Coulomb force, leading to complex deformation and motion of the droplet while at the same time inducing a bulk electroconvective flow. After we validate the model by comparing with analytical solutions at the hydrostatic state, we perform a quantitative analysis on the droplet deformation factor D and bulk flow stability criteria T_{c} under different parameters, including the electric capillary number Ca, the electric Rayleigh number T, the permittivity ratio ɛ_{r}, and the mobility ratio K_{r}. It is found that the bulk flow significantly modifies the magnitude of D, which in turn decreases T_{c} of the electroconvective flow. For a droplet repelled by the anode, ɛ_{r}>1, an interesting linear relationship can be observed in the D-Ca curves. However, for a droplet attracted to the anode, ɛ_{r}<1, the system is potentially unstable. After first evolving into a quasisteady state, the droplet successively experiences steady flow, periodic flow, second steady flow, and oscillatory flow with increasing T. Moreover, discontinuities can be observed in the D-T curves due to the transitions of bulk flow.
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http://dx.doi.org/10.1103/PhysRevE.105.065304DOI Listing
June 2022

Machine learning and radiomics for the prediction of multidrug resistance in cavitary pulmonary tuberculosis: a multicentre study.

Eur Radiol 2022 Jul 19. Epub 2022 Jul 19.

Department of Radiology, Beijing Chest Hospital, Capital Medical University, Beijing, 101149, China.

Objectives: Multidrug-resistant tuberculosis (MDR-TB) is a major challenge to global health security. Early identification of MDR-TB patients increases the likelihood of treatment success and interrupts transmission. We aimed to develop a predictive model for MDR to cavitary pulmonary TB using CT radiomics features.

Methods: This retrospective study included 257 consecutive patients with proven active cavitary TB (training cohort: 187 patients from Beijing Chest Hospital; testing cohort: 70 patients from Infectious Disease Hospital of Heilongjiang Province). Radiomics features were extracted from the segmented cavitation. A radiomics model was constructed to predict MDR using a random forest classifier. Meaningful clinical characteristics and subjective CT findings comprised the clinical model. The radiomics and clinical models were combined to create a combined model. ROC curves were used to validate the capability of the models in the training and testing cohorts.

Results: Twenty-one radiomics features were selected as optimal predictors to build the model for predicting MDR-TB. The AUCs of the radiomics model were significantly higher than those of the clinical model in either the training cohort (0.844 versus 0.589, p < 0.05) or the testing cohort (0.829 versus 0.500, p < 0.05). The AUCs of the radiomics model were slightly lower than those of the combined model in the training cohort (0.844 versus 0.881, p > 0.05) and testing cohort (0.829 versus 0.834, p > 0.05), but there was no significant difference.

Conclusions: The radiomics model has the potential to predict MDR in cavitary TB patients and thus has the potential to be a diagnostic tool.

Key Points: • This is the first study to build and validate models that distinguish MDR-TB from DS-TB with clinical and radiomics features based on cavitation. • The radiomics model demonstrated good performance and might potentially aid in prior TB characterisation treatment. • This noninvasive and convenient technique can be used as a diagnosis tool into routine clinical practice.
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http://dx.doi.org/10.1007/s00330-022-08997-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294743PMC
July 2022

Identification of three different phenotypes in anti-MDA5 antibody-positive dermatomyositis patients: implications for rapidly progressive interstitial lung disease prediction.

Arthritis Rheumatol 2022 Jul 18. Epub 2022 Jul 18.

Department of Rheumatology, The First Affiliated Hospital of Nanjing Medical University, China, Nanjing, Jiangsu, China.

Objective: Substantial phenotypic heterogeneity exists in anti-melanoma differentiation-associated gene 5 antibody-positive (anti-MDA5 ) dermatomyositis patients, hindering disease assessment and management. This study aimed to identify distinct phenotypic groups in anti-MDA5 patients and determine their utility in outcome prediction.

Methods: A total of 265 patients with anti-MDA5 DM were enrolled retrospectively. An unsupervised analysis was performed to characterize the phenotypes.

Results: Three clusters with markedly different features and outcomes (all-cause mortality rates of 60%, 9.7%, and 3.7% for clusters 3, 2 and 1 (p < 0.0001)) were identified. Cluster 1 (n = 108) corresponded to mild risk of rapidly progressive ILD (RPILD) with the non-RPILD cumulative incidence being 85.2%. Cluster 2 (n = 72) corresponded to moderate risk of RPILD with a non-RPILPD cumulative incidence of 73.6%. Cluster 3 (n = 85), corresponding to high risk of RPILD with a non-RPILD cumulative incidence of 32.9%, had the highest possibility for anti-Ro52 antibodies to coexist with high titers of anti-MDA5 antibodies among the 3 subgroups. Decision tree analysis indicated a simple algorithm comprising of eight variables (age > 50 years, disease course < 3 months, myasthenia (proximal muscle weakness), arthritis, CRP level, CK level, anti-Ro52 and anti-MDA5 antibody titers), which could place patients into the appropriate clusters with 78.5% and 70.0% accuracies in the development and external validation cohorts.

Conclusion: Cluster analysis identified 3 distinct clinical patterns and outcomes in our large cohort of anti-MDA5 DM patients. Classification of these patients into phenotypes with prognostic values may help physicians to improve the efficacy of clinical decisions.
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http://dx.doi.org/10.1002/art.42308DOI Listing
July 2022

Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex.

PLoS Genet 2022 Jul 18;18(7):e1010308. Epub 2022 Jul 18.

Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The conserved shelterin complex caps chromosome ends to protect telomeres and regulate telomere replication. In fission yeast Schizosaccharomyces pombe, shelterin consists of telomeric single- and double-stranded DNA-binding modules Pot1-Tpz1 and Taz1-Rap1 connected by Poz1, and a specific component Ccq1. While individual structures of the two DNA-binding OB folds of Pot1 (Pot1OB1-GGTTAC and Pot1OB2-GGTTACGGT) are available, structural insight into recognition of telomeric repeats with spacers by the complete DNA-binding domain (Pot1DBD) remains an open question. Moreover, structural information about the Tpz1-Ccq1 interaction requires to be revealed for understanding how the specific component Ccq1 of S. pombe shelterin is recruited to telomeres to function as an interacting hub. Here, we report the crystal structures of Pot1DBD-single-stranded-DNA, Pot1372-555-Tpz1185-212 and Tpz1425-470-Ccq1123-439 complexes and propose an integrated model depicting the assembly mechanism of the shelterin complex at telomeres. The structure of Pot1DBD-DNA unveils how Pot1 recognizes S. pombe degenerate telomeric sequences. Our analyses of Tpz1-Ccq1 reveal structural basis for the essential role of the Tpz1-Ccq1 interaction in telomere recruitment of Ccq1 that is required for telomere maintenance and telomeric heterochromatin formation. Overall, our findings provide valuable structural information regarding interactions within fission yeast shelterin complex at 3' ss telomeric overhang.
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http://dx.doi.org/10.1371/journal.pgen.1010308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9333443PMC
July 2022

Predictors of improvement in disease activity in first hospitalized patients with systemic lupus erythematosus: a multicenter retrospective study of a Chinese cohort.

Clin Rheumatol 2022 Jul 18. Epub 2022 Jul 18.

Department of Rheumatology and Immunology, Affiliated Nanjing Drum Tower Hospital, Medical School of Nanjing University, 321 Zhongshan Road, Nanjing, 210008, China.

Objectives: To analyze the relative factors of improvement in disease activity (IDA) after first hospitalized treatment based on the systemic lupus erythematosus disease activity index (SLEDAI).

Methods: A total of 1069 adult systemic lupus erythematosus (SLE) patients who were hospitalized for the first time in 26 hospitals in Jiangsu Province from 1999 to 2009 were retrospectively analyzed. SLEDAI decrease ≥ 4 during hospitalization was identified as IDA. Relative factors of IDA were assessed by univariate and multivariate logistic regression.

Results: A total of 783 (73.2%) adult SLE patients showed IDA after the first hospitalization, while the remaining patients (n = 286) were in the non-IDA group. The IDA group had higher SLEDAI at admission; fewer patients had SLICC/ACR damage index (SDI) ≥ 1, comorbidities at admission, especially Sjögren's syndrome, abnormal serum creatinine, and glomerular filtration rate. More patients had mucocutaneous and musculoskeletal involvements, leukopenia, increased C-reactive protein, anti-dsDNA antibody positive, and hypocomplementemia at admission and were treated with methotrexate and leflunomide during hospitalization. After multivariate logistic regression analysis, SDI ≥ 1 (P = 0.005) and combined with Sjögren's syndrome (P < 0.001) at admission had negative association with IDA. Musculoskeletal involvement (P < 0.001), anti-dsDNA antibody positive (P = 0.012), hypocomplementemia (P = 0.001), and use of leflunomide (P = 0.030) were significantly related with IDA.

Conclusion: Organ damage or comorbidities at admission were adverse to SLE improvement. Anti-dsDNA antibody positive, hypocomplementemia, musculoskeletal involvements, and leflunomide treatment had positive association with IDA of SLE. Key Points • Organ damage or comorbidities at admission were negatively correlated with SLE improvement. • Anti-dsDNA antibody positivity, hypocomplementemia, musculoskeletal involvements, and leflunomide treatment were positively associated with SLE improvement.
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http://dx.doi.org/10.1007/s10067-022-06289-7DOI Listing
July 2022

The therapeutic effect of stem cells from human exfoliated deciduous teeth on a rat model of tracheal fistula.

Stem Cell Res Ther 2022 07 15;13(1):310. Epub 2022 Jul 15.

School of Medicine, South China University of Technology, Guangzhou, 510006, China.

Background: Tracheal fistulas (TF) can be dangerous and even fatal in patients. The current treatment is really challenging. Previous studies reported that mesenchymal stem cells (MSCs) could be used to treat respiratory tract fistulas. Stem cells from human exfoliated deciduous teeth (SHED) are considered to be MSC-like cells that may also have the potential to treat the tracheal fistulas. In this study, we investigated the therapeutic effects of SHED in rat tracheal fistula models.

Methods: A total of 80 SD rats were randomly divided into five groups: a sham-operated group, a local PBS group (L-PBS), an intravenous PBS group (I-PBS), a local SHED treatment group (L-SHED), and an intravenous SHED treatment group (I-SHED). The L-SHED and I-SHED groups were given a topical application around the fistula or an intravenous injection of 1*10 SHED via the tail vein, respectively, while the L-PBS and I-PBS groups were given an equivalent volume of PBS through local or intravenous administration. A stereomicroscope was used to observe fistula healing on the 2nd, 3rd, and 5th days following transplantation. On the 7th day, the survival of SHED was observed by immunofluorescence. The pathology of the lungs and fistulas was observed by hematoxylin and eosin (H&E) and Masson staining. The expression levels of the Toll-like receptor 4 (TLR4), interleukin (IL)-1β, IL-33, and IL-4 were measured using immunohistochemistry. The expression levels of TLR4, high mobility group box 1 (HMGB1), and myeloid differentiation factor 88 (MYD88) were studied using western blotting. On day 14, airway responsiveness of rats was detected and analyzed.

Results: Fistula healing in the L-SHED and I-SHED groups was faster than that in their respective PBS groups after transplantation. The fistula diameters in the L-SHED and I-SHED groups were significantly smaller than those in the L-PBS and I-PBS groups on the 3rd day. Moreover, the phenomenon of fibroblast proliferation and new blood vessel growth around the fistula seemed more pronounced in the L-SHED and I-SHED groups. Although no discernible difference was found in airway responsiveness after SHED treatment, the degree of inflammation in the lungs was reduced by intravenous SHED treatment. However, there was no significant reduction in lung inflammation by local SHED treatment. The expression levels of IL-1β and IL-33 were decreased in the I-SHED group, while IL-4 was elevated compared with the I-PBS group. Interestingly, intravenous SHED treatment inhibited the activation of HMGB1/TLR4/MYD88 in the lung tissues of TF rats.

Conclusions: SHED transplantation accelerated the rate of fistula healing in rats. Intravenous SHED treatment reduced lung inflammation. Thus, SHED may have potential in the treatment of tracheal fistula, providing hope for future therapeutic development for TF.
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http://dx.doi.org/10.1186/s13287-022-02994-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284811PMC
July 2022
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