Publications by authors named "Jian Tian"

293 Publications

Spatiotemporal distribution and mass loading of organophosphate flame retardants (OPFRs) in the Yellow River of China (Henan segment).

Environ Pollut 2021 Aug 18;290:118000. Epub 2021 Aug 18.

School of Environment, Henan Normal University, Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, Henan Key Laboratory for Environmental Pollution Control, Xinxiang, Henan, 453007, PR China.

During three sampling periods in 2014, systematic investigations were conducted into contamination profiles of ten organophosphate flame retardants (OPFRs) in both suspended particulate phase and water phase in the Yellow River (Henan Area). This research shows that OPFRs exist at lower concentrations in the suspended phase than in the water phase. The median concentration of 10 OPFRs (∑OPFRs) in the suspended particulate phase was 62.5 ng/g (fluctuating from ND to 6.17 × 10 ng/g, dw), while their median concentration in the water phase was 109 ng/L (fluctuating from 35.6 to 469 ng/L). Among the selected 10 OPFRs, triethylphosphate (TEP), tris(1-chloro-2-propyl) phosphate (TCPP), and tris(2-chloroethyl) phosphate (TCEP) were the predominant compounds in the water phase (occupying 91.6% of the ∑OPFRs), while TCPP, TCEP, and tri-o-tolyl phosphate (o-TCP) were the most common in the suspended particulate phase, accounting for 90.1% of the ∑OPFRs. Across the three sampling periods, there was no significant seasonable variation for OPFRs either in the water phase or in the suspended particulate phase, except for TCEP and TCPP in the water phase. Compared with research findings relating to concentrations of OPFRs around China and abroad, the OPFRs of the Yellow River (Henan Area) in the water phase were at a moderate level. Suspended particles (SS) had a very important impact on the transportation of OPFRs in the studied area, with about 83.9% of ∑OPFRs inflow attributed to SS inflow and about 81.7% of ∑OPFRs outflow attributed to SS outflow. The total annual inflow and outflow of OPFRs were 7.72 × 10 kg and 6.62 × 10 kg in the studied area, respectively.
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http://dx.doi.org/10.1016/j.envpol.2021.118000DOI Listing
August 2021

Direct Bilirubin Levels Predict Long-Term Outcomes in Patients With Acute Coronary Syndrome Under Different Glucose Metabolism Status: A 6.5-Year Cohort Study of Three-Vessel Disease.

Front Cardiovasc Med 2021 12;8:715539. Epub 2021 Aug 12.

Department of Cardiology, Fuwai Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.

There is controversy over the relationship between bilirubin and coronary artery disease. This study aimed to evaluate the predictive value of direct bilirubin (DB) in patients with complex acute coronary syndrome (ACS). From April 2004 to February 2011, 5,322 ACS patients presenting with three-vessel disease were consecutively enrolled. Disease severity and complexity were determined by SYNTAX score (SS) and SS II. The primary endpoint was all-cause death, and the secondary endpoints were major adverse cardiovascular and cerebrovascular events (MACCE). Stratification of normal glucose regulation, prediabetes, and diabetes was based on a previous diagnosis, hypoglycemic medications, fasting blood glucose, and hemoglobin A1c. Subjects were divided into quartiles according to baseline DB (μmol/L): Q1 (0-1.6), Q2 (1.61-2.20), Q3 (2.21-2.80), and Q4 (>2.80). Multivariable logistic regression analysis showed that DB was an independent predictor of intermediate-high SS. During a median follow-up time of 6.5 years, elevated DB was associated with more all-cause death ( < 0.001) but not MACCE. DB remained to be predictive of all-cause death in the multivariable Cox regression model (Q2 vs. Q1: HR 1.043, 95% CI 0.829-1.312, = 0.719; Q3 vs. Q1: HR 1.248, 95% CI 1.001-1.155, = 0.048; Q4 vs. Q1: HR 1.312, 95% CI 1.063-1.620, = 0.011). When subjects are stratified according to glucose metabolism regulation and treatment strategies, the predictivity of DB was only profound in patients with diabetes or with conservative treatment. Additionally, incorporating DB further improved the discrimination and reclassification abilities of SS II for risk prediction. DB is a potential biomarker for predicting lesion severity and long-term outcomes in ACS patients.
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http://dx.doi.org/10.3389/fcvm.2021.715539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387870PMC
August 2021

Cadmium Pollution Impact on the Bacterial Community Structure of Arable Soil and the Isolation of the Cadmium Resistant Bacteria.

Front Microbiol 2021 22;12:698834. Epub 2021 Jul 22.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.

Microorganisms play an important role in the remediation of cadmium pollution in the soil and their diversity can be affected by cadmium. In this study, the bacterial community in arable soil samples collected from two near geographical sites, with different degrees of cadmium pollution at three different seasons, were characterized using Illumina MiSeq sequencing. The result showed that cadmium is an important factor to affect the bacterial diversity and the microbial communities in the high cadmium polluted area (the site H) had significant differences compared with low cadmium polluted area (the site L). Especially, higher concentrations of Cd significantly increased the abundance of Proteobacteria and Gemmatimonas whereas decreased the abundance of Nitrospirae. Moreover, 42 Cd-resistant bacteria were isolated from six soil samples and evaluated for potential application in Cd bioremediation. Based on their Cd-MIC [minimum inhibitory concentration (MIC) of Cd], Cd removal rate and 16S rDNA gene sequence analyses, three sp. strains (ha-1, hj-2, and ho-3) showed very high tolerance to Cd (5, 5, and 6 mM) and exhibited high Cd removal rate (81.78, 79.37, and 63.05%), six sp. strains (151-5,151-6,151-13, 151-20, and 151-21) showed moderate tolerance to Cd (0.8, 0.4, 0.8, 0.4, 0.6, and 0.4 mM) but high Cd removal rate (84.78, 90.14, 82.82, 82.39, 81.79, and 84.17%). Those results indicated that sp. belonging to the phylum Proteobacteria and sp. belonging to the phylum Firmicutes have developed a resistance for cadmium and may play an important role in Cd-contaminated soils. Our study provided baseline data for bacterial communities in cadmium polluted soils and concluded that Cd-resistant bacteria have potential for bioremediation of Cd-contaminated soils.
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http://dx.doi.org/10.3389/fmicb.2021.698834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339475PMC
July 2021

Phosphorous-doped 1T-MoS decorated nitrogen-doped g-CN nanosheets for enhanced photocatalytic nitrogen fixation.

J Colloid Interface Sci 2021 Jul 24;605:320-329. Epub 2021 Jul 24.

School of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, China. Electronic address:

Herein, we report that the phosphorous-doped 1 T-MoS as co-catalyst decorated nitrogen-doped g-CN nanosheets (P-1 [email protected]) are prepared by the hydrothermal and annealing process. The obtained P-1 [email protected] composite presents an enhanced photocatalytic N reduction rate of 689.76 μmol L gh in deionized water without sacrificial agent under simulated sunlight irradiation, which is higher than that of pure g-CN (265.62 μmol L gh), 1 [email protected] (415.57 μmol L gh), 1 [email protected] doped g-CN (469.84 μmol L gh), and P doped 1 [email protected] (531.24 μmol L gh). In addition, compared with pure g-CN NSs (2.64 mmol L gh), 1 [email protected] (4.98 mmol L gh), 1 [email protected] doped g-CN (6.21 mmol L gh), and P doped 1 [email protected] (9.78 mmol L gh), P-1 [email protected] (11.12 mmol L gh) composite also shows a significant improvement for photocatalytic N fixation efficiency in the sacrificial agent (methanol). The improved photocatalytic activity of P-1 [email protected] composite is ascribed to the following advantages: 1) Compared to pure g-CN, P-1 [email protected] composite shows higher light absorption capacity, which can improve the utilization rate of the catalyst to light; 2) The P doping intercalation strategy can promote the conversion of 1 T phase MoS, which in turn in favor of photogenerated electron transfer and reduce the recombination rate of carriers; 3) A large number of active sites on the edge of 1 T-MoS and the existence of N doping in g-CN contribute to photocatalytic N fixation.
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http://dx.doi.org/10.1016/j.jcis.2021.07.111DOI Listing
July 2021

Nitrogen-functionalized carbon nanotube-supported bimetallic PtNi nanoparticles for hydrogen generation from hydrous hydrazine.

Chem Commun (Camb) 2021 Aug 29;57(67):8324-8327. Epub 2021 Jul 29.

School of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, China.

Herein, ultrafine PtNi nanoparticles (NPs) supported on N-functionalized multi-walled carbon nanotubes (N-MWCNTs) were facilely obtained. The N species on N-MWCNTs not only benefit the formation of PtNi NPs but also accelerate the dehydrogenation of hydrous hydrazine, affording a high turnover frequency of 1595 h with 100% H selectivity. In addition, the N species can be used as an "anchor" to stabilize the bimetallic NPs and efficiently restrain the aggregation, giving robust stability of the catalysts for decomposition of hydrous hydrazine over PtNi/N-MWCNTs.
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http://dx.doi.org/10.1039/d1cc02794hDOI Listing
August 2021

A deep learning approach to automate whole-genome prediction of diverse epigenomic modifications in plants.

New Phytol 2021 Jul 20. Epub 2021 Jul 20.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Epigenetic modifications function in gene transcription, RNA metabolism, and other biological processes. However, multiple factors currently limit the scientific utility of epigenomic datasets generated for plants. Here, using deep-learning approaches, we developed a Smart Model for Epigenetics in Plants (SMEP) to predict six types of epigenomic modifications: DNA 5-methylcytosine (5mC) and N6-methyladenosine (6mA) methylation, RNA N6-methyladenosine (m A) methylation, and three types of histone modification. Using the datasets from the japonica rice Nipponbare, SMEP achieved 95% prediction accuracy for 6mA, and also achieved around 80% for 5mC, m A, and the three types of histone modification based on the 10-fold cross-validation. Additionally, > 95% of the 6mA peaks detected after a heat-shock treatment were predicted. We also successfully applied the SMEP for examining epigenomic modifications in indica rice 93-11 and even the B73 maize line. Taken together, we show that the deep-learning-enabled SMEP can reliably mine epigenomic datasets from diverse plants to yield actionable insights about epigenomic sites. Thus, our work opens new avenues for the application of predictive tools to facilitate functional research, and will almost certainly increase the efficiency of genome engineering efforts.
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http://dx.doi.org/10.1111/nph.17630DOI Listing
July 2021

Optimal Sensor Placement of the Artificial Lateral Line for Flow Parametric Identification.

Sensors (Basel) 2021 Jun 9;21(12). Epub 2021 Jun 9.

School of Reliability and System Engineering, Beihang University, No.37 Xueyuan Road, Beijing 100191, China.

The multi-sensor artificial lateral line system (ALLS) can identify the flow-field's parameters to realize the closed-loop control of the underwater robotic fish. An inappropriate sensor placement of ALLS may result in inaccurate flow-field parametric identification. Therefore, this paper proposes a method to optimize the sensor placement configuration of the ALLS, which mainly included three algorithms, the feature importance algorithm based on mean and variance (MVF), the feature importance algorithm based on distance evaluation (DF), and the information redundancy (IR) algorithm. The optimal sensor placement performance selected by this method is verified by simulation. In addition, further experimental verification was conducted using the ALLS. Compared with the uniform sensor placement configuration mentioned in recent studies, the experimental results suggest that the optimal sensor placement method can achieve a more effective prediction of the flow-field parameters, therefore strengthening the underwater robotic fish's perception and control function.
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http://dx.doi.org/10.3390/s21123980DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228240PMC
June 2021

Relationship Between High-Sensitivity C-Reactive Protein and Long-Term Outcomes in Elderly Patients With 3-Vessel Disease.

Angiology 2021 Jun 10:33197211021195. Epub 2021 Jun 10.

34736Fuwai Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, People's Republic of China.

The prognostic value of high-sensitivity C-reactive protein (hsCRP) in complex coronary artery disease has not been fully established. We aimed to determine the association between hsCRP and long-term outcomes in elderly patients with 3-vessel disease (TVD). From April 2004 to February 2011, 3069 patients aged ≥65 years with TVD were consecutively enrolled and received medical treatment alone, percutaneous coronary intervention, or coronary artery bypass grafting. The patients were divided into 2 groups according to their hsCRP levels: <3.00 mg/L (62.1%) and ≥3.00 mg/L (37.9%). The mean age was 71 ± 4 years. The high hsCRP group had more risk factors and more frequently received conservative treatment than the low hsCRP group. During a median follow-up period of 6.2 years, elevated hsCRP was significantly associated with increased all-cause death (19.5% vs 29.6%, < .001), cardiac death (9.4% vs 15.2%, = .001), and major adverse cardiovascular and cerebrovascular events (34.1% vs 42.5%, = .001). Multivariable Cox regression analyses revealed that hsCRP was an independent predictor for all of these events. Combining hsCRP with Synergy between PCI with TAXUS and Cardiac Surgery score II further improved the predictive power of the score. The relationship between hsCRP and mortality was relatively consistent across subgroups. Overall, hsCRP could prove useful for risk prediction in elderly patients.
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http://dx.doi.org/10.1177/00033197211021195DOI Listing
June 2021

The epidemiology of blood transfusion in hospitalized children: a national cross-sectional study in China.

Transl Pediatr 2021 Apr;10(4):1055-1062

Big Data and Engineering Research Center, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

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http://dx.doi.org/10.21037/tp-20-464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107874PMC
April 2021

Assessment of causal association between thyroid function and lipid metabolism: a Mendelian randomization study.

Chin Med J (Engl) 2021 Apr 13;134(9):1064-1069. Epub 2021 Apr 13.

Department of Cardiology, Peking University Third Hospital, Beijing 100191, China.

Background: Thyroid dysfunction is associated with cardiovascular diseases. However, the role of thyroid function in lipid metabolism remains partly unknown. The present study aimed to investigate the causal association between thyroid function and serum lipid metabolism via a genetic analysis termed Mendelian randomization (MR).

Methods: The MR approach uses a genetic variant as the instrumental variable in epidemiological studies to mimic a randomized controlled trial. A two-sample MR was performed to assess the causal association, using summary statistics from the Atrial Fibrillation Genetics Consortium (n = 537,409) and the Global Lipids Genetics Consortium (n = 188,577). The clinical measures of thyroid function include thyrotropin (TSH), free triiodothyronine (FT3) and free thyroxine (FT4) levels, FT3:FT4 ratio and concentration of thyroid peroxidase antibodies (TPOAb). The serum lipid metabolism traits include total cholesterol (TC) and triglycerides, high-density lipoprotein, and low-density lipoprotein (LDL) levels. The MR estimate and MR inverse variance-weighted method were used to assess the association between thyroid function and serum lipid metabolism.

Results: The results demonstrated that increased TSH levels were significantly associated with higher TC (β = 0.052, P = 0.002) and LDL (β = 0.041, P = 0.018) levels. In addition, the FT3:FT4 ratio was significantly associated with TC (β = 0.240, P = 0.033) and LDL (β = 0.025, P = 0.027) levels. However, no significant differences were observed between genetically predicted FT4 and TPOAb and serum lipids.

Conclusion: Taken together, the results of the present study suggest an association between thyroid function and serum lipid metabolism, highlighting the importance of the pituitary-thyroid-cardiac axis in dyslipidemia susceptibility.
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http://dx.doi.org/10.1097/CM9.0000000000001505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116035PMC
April 2021

Stent-Assisted Coiling of a Ruptured Supraclinoid Internal Carotid Artery Aneurysm in Tetralogy of Fallot Under Conscious Sedation.

Ann Indian Acad Neurol 2021 Jan-Feb;24(1):116-118. Epub 2020 Sep 2.

Department of Neurosurgery, Beijing Tsinghua Changgung Hospital, Tsinghua University School of Clinical Medicine, Beijing, China.

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http://dx.doi.org/10.4103/aian.AIAN_142_20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061513PMC
September 2020

Facile preparation of metallic 1T phase molybdenum selenide as cocatalyst coupled with graphitic carbon nitride for enhanced photocatalytic H production.

J Colloid Interface Sci 2021 Sep 17;598:172-180. Epub 2021 Apr 17.

School of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, China. Electronic address:

Low-cost, highly active and efficient alternative co-catalysts that can replace precious metals such as Au and Pt are urgently needed for photocatalytic hydrogen evolution reaction (HER). Herein, we show that 1T phase MoSe can act as the co-catalyst in the 1T-MoSe/g-CN composites and we synthesize this composite by a one-step hydrothermal method to promote photocatalytic H generation. Our prepared 1T-MoSe/g-CN composite exhibits highly enhanced photocatalytic H production compared to that of g-CN nanosheets (NSs) only. The 7 wt%-1T-MoSe/g-CN composite presents a considerably improved photocatalytic HER rate (6.95 mmol·h·g), approximately 90 times greater than that of pure g-CN (0.07 mmol·h g). Moreover, under illumination at λ = 370 nm, the apparent quantum efficiency (AQE) of the 7 wt%-1T-MoSe/g-CN composite reaches 14.0%. Furthermore, the 1T-MoSe/g-CN composites still maintain outstanding photocatalytic HER stability.
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http://dx.doi.org/10.1016/j.jcis.2021.04.066DOI Listing
September 2021

Recent Advances in Catalyst Structure and Composition Engineering Strategies for Regulating CO Electrochemical Reduction.

Adv Mater 2021 Apr 25:e2005484. Epub 2021 Apr 25.

Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.

Electrochemical CO reduction has been recognized as a promising solution in tackling energy- and environment-related challenges of human society. In the past few years, the rapid development of advanced electrocatalysts has significantly improved the efficiency of this reaction and accelerated the practical applications of this technology. Herein, representative catalyst structures and composition engineering strategies in regulating the CO reduction selectivity and activity toward various products including carbon monoxide, formate, methane, methanol, ethylene, and ethanol are summarized. An overview of in situ/operando characterizations and advanced computational modeling in deepening the understanding of the reaction mechanisms and accelerating catalyst design are also provided. To conclude, future challenges and opportunities in this research field are discussed.
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http://dx.doi.org/10.1002/adma.202005484DOI Listing
April 2021

Defect-engineered porphyrinic metal-organic framework nanoparticles for targeted multimodal cancer phototheranostics.

Chem Commun (Camb) 2021 Apr;57(33):4035-4038

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (MOE), School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.

Defect-engineered porphyrinic MOF nanoparticles were fabricated with an in situ one-pot protocol using cypate as the co-ligand and modulator. This multifunctional nanoplatform integrated the photothermal and multimodal imaging properties of cypate with the photodynamic effects of porphyrins, thus achieving targeted multimodal cancer phototheranostics after folic acid modification.
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http://dx.doi.org/10.1039/d0cc07903kDOI Listing
April 2021

Sphingosine 1-phosphate and osteoporosis: pathophysiology and therapeutic aspects-a narrative review.

Ann Palliat Med 2021 Apr 26;10(4):4799-4805. Epub 2021 Mar 26.

Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, China; National Clinical Research Centre for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China.

Sphingosine 1-phosphate (S1P) regulates many cellular functions, such as differentiation, proliferation, migration, morphogenesis, cytoskeletal organization, adhesion, tight junction assembly, apoptosis and the localization of different cell types. S1P also controls the migration of osteoclast precursors between the blood and bone, and it keeps osteoclast precursors away from bone surfaces to reduce bone degradation, thus preventing bone decay. Osteoporosis is a systemic bone disease that predisposes patients to bone fracture due to decreased bone density and quality, disrupted bone microarchitecture, and increased bone fragility. As the global elderly population increases, the incidence of osteoporosis will greatly increase, and the associated adverse consequences will become more serious. S1P plays an important role in homeostasis, and disruption of the balance between osteoblasts and osteoclasts may induce osteoporosis. A high frequency of osteoporotic fracture is associated with increased plasma S1P levels. Studies have shown that S1P is an important therapeutic target in osteoporosis because it controls the migration of osteoclast precursors, vigorously maintains the bone mineralization process, and is a critical regulator of osteoclastogenesis. Improved understanding of the functional roles and molecular mechanisms of S1P in bone turnover could facilitate the discovery of novel targets for the treatment of osteoporosis. This review provides a critical discussion of the role of S1P in osteoporosis and treatments.
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http://dx.doi.org/10.21037/apm-20-1255DOI Listing
April 2021

Protein engineering of stable IsPETase for PET plastic degradation by Premuse.

Int J Biol Macromol 2021 Jun 19;180:667-676. Epub 2021 Mar 19.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address:

Poly(ethylene terephthalate) (PET) is used widely by human beings, but is very difficult to degrade. Up to now, the PET degradation effect of PETase from Ideonella sakaiensis 201-F6 (IsPETase) variants with low stability and activity was not ideal. In this study, a mutation design tool, Premuse, was developed to integrate the sequence alignment and quantitative selection of the preferred mutations based on natural sequence evolution. Ten single point mutants were selected from 1486 homologous sequences using Premuse, and then two mutations (W159H and F229Y) with improved stability were screened from them. The derived double point mutant, W159H/F229Y, exhibited a strikingly enhanced enzymatic performance. Its T and catalytic efficiency values (k/K) respectively increased by 10.4 °C and 2.0-fold using p-NPP as the substrate compared with wild type. The degradation activity for amorphous PET was increased by almost 40-fold in comparison with wild type at 40 °C in 24 h. Additionally, the variant could catalyze biodegradation of PET bottle preform at a mean rate of 23.4 mg/h/mg. This study allowed us to design the mutation more efficiently, and provides a tool for achieving biodegradation of PET pollution under mild natural environments.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.058DOI Listing
June 2021

WISP1 Protects Against Chondrocyte Senescence and Apoptosis by Regulating αvβ3 and PI3K/Akt Pathway in Osteoarthritis.

DNA Cell Biol 2021 Apr 1;40(4):629-637. Epub 2021 Mar 1.

Department of Orthopaedics, Yiyang Central Hospital, Yiyang, China.

Our study aimed at validating the effect of WISP1 on osteoarthritis (OA) and the pathway involved in the WISP1-induced protection against OA. The expression of WISP1 was measured by immunohistochemical analyses. We found that WISP1 expression was shown to be upregulated within human OA cartilage compared with controls. WISP1 expression was related to knee OA severity. rhWISP1 inhibited OA chondrocyte senescence and apoptosis , which was reversed by the αvβ3 antibody and PI3K/Akt inhibitor LY294002. WISP1 overexpression induced by knee injection of LiCI could also prevent the senescence and apoptosis of rat chondrocytes. Safranin-O staining and Mankin score revealed that WISP1 overexpression can protect rat chondrocytes from degeneration. Nearly opposite results were obtained in the treatment of ICG-001 and siRNA-WISP1 . These data strongly suggest that WISP1 can protect against the senescence and apoptosis of chondrocytes via modulating the αvβ3 receptor and PI3K/Akt signaling pathway within OA. Therefore, the development of specific activators of WISP1 may present the value of an underlying OA treatment.
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http://dx.doi.org/10.1089/dna.2020.5926DOI Listing
April 2021

The associations between serum trace elements and bone mineral density in children under 3 years of age.

Sci Rep 2021 01 21;11(1):1890. Epub 2021 Jan 21.

Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, 410008, China.

We examined the associations of age and serum magnesium, iron, lead, copper, and zinc levels with bone mineral density (BMD) in 2412 children under 3 years of age in order to find a tool to monitor BMD in children without the use of expensive imaging techniques. One-way ANOVA and chi-square tests were used to determine the associations of age and serum trace elements with BMD. Multivariable logistic regression analysis was used to test the correlation of five serum trace elements with BMD after adjustments for potential confounding factors in children under 3 years of age. Significant associations between age and four serum trace elements and BMD were found. Compared to the group with the lowest serum levels detected, the adjusted odds ratio (OR) for the incidence of normal bone mineral density in the third magnesium concentration tertile, the third iron concentration tertile, the fifth copper concentration quintile, the third zinc concentration quintile, and the fifth zinc concentration quintile were 1.30 (95% confidence interval (CI) 1.02-1.67), 1.43 (95% CI 1.11-1.84), 1.42 (95% CI 1.04-1.94), 1.46 (95% CI 1.05-2.04), and 1.48 (95% CI 1.06-2.06), respectively. However, there was no significant correlation between serum lead level and BMD in this study. Age and serum magnesium, iron, copper, and zinc levels are positively associated with BMD in children under 3 years old.
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http://dx.doi.org/10.1038/s41598-021-81501-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820346PMC
January 2021

One-Pot Fabrication of Hollow Porphyrinic MOF Nanoparticles with Ultrahigh Drug Loading toward Controlled Delivery and Synergistic Cancer Therapy.

ACS Appl Mater Interfaces 2021 Jan 19;13(3):3679-3693. Epub 2021 Jan 19.

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (MOE), Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, P. R. China.

Hollow nanostructures have attracted significant research interest in drug delivery systems due to their high capacities for drug loading and unique physicochemical properties, showing great potential in specific biomedical applications. Herein, hollow porphyrinic metal-organic framework (H-PMOF) nanoparticles with a mesoporous spherical shell have been fabricated a facile self-sacrificial ZIF-8 nanoparticle template strategy. The H-PMOF nanoplatform not only demonstrates a greatly enhanced photodynamic therapy efficacy compared with nonhollow porphyrinic MOF nanoparticles but also can be used as a superior drug carrier to co-load doxorubicin (DOX) and indocyanine green (ICG) with an ultrahigh drug-loading capacity of 635%. Furthermore, cancer cell membrane camouflage of the (DOX and ICG)@H-PMOF composite nanoparticles affords a biomimetic nanoplatform, that is, (DOX and ICG)@[email protected] (DIHPm for short), with an outstanding homologous tumor-targeting and immune-escaping ability. Interestingly, DIHPm shows both pH-controlled and near-infrared laser-triggered DOX release. Both and studies of DIHPm demonstrate an excellent imaging-guided synergistic photodynamic/photothermal/chemotherapy anticancer activity with negligible systemic toxicity. The development of the high-performance H-PMOF nanoplatform provides new insights into the design of MOF-based multifunctional nanomedicines for combination cancer therapy and precise theranostics.
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http://dx.doi.org/10.1021/acsami.0c20617DOI Listing
January 2021

Real-world outcomes of different treatment strategies in patients with diabetes and three-vessel coronary disease: a mean follow-up 6.3 years study from China.

Cardiovasc Diabetol 2021 01 11;20(1):16. Epub 2021 Jan 11.

State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 167 Beilishi Road, Xicheng District, Beijing, China.

Background: Patients with diabetes and triple-vessel disease (TVD) are associated with a high risk of events. The choice of treatment strategies remains a subject of discussion. In the real-world, we aim to compare the outcomes of medical therapy (MT), coronary artery bypass grafting (CABG), and percutaneous coronary intervention (PCI) treatment strategies in patients with diabetes and TVD.

Methods: A total of 3117 consecutive patients with diabetes and TVD were enrolled. The primary endpoint was all-cause death and the secondary endpoint was major adverse cardiac and cerebrovascular events (MACCE, composite of all-cause death, myocardial infarction, or stroke).

Results: During the mean follow-up of 6.3 ± 2.6 years, 573 (18.4%) deaths and 1094 (35.1%) MACCE occurred. Multivariate analysis showed that PCI (hazard ratio [HR] 0.40, 95% confidence interval [CI] 0.32-0.51) and CABG (HR 0.33, 95% CI 0.26-0.44) were associated with a lower risk of death compared with MT, with no difference between the PCI and CABG groups. When MACCE was the endpoint, PCI (HR 0.71, 95% CI 0.60-0.84) and CABG (HR 0.48, 95% CI 0.39-0.57) had a lower risk than MT. CABG was associated with a significantly lower risk of MACCE compared with PCI (HR 0.67, 95% CI 0.55-0.81), which was mainly attributed a lower risk in myocardial infarction, but a higher risk of stroke.

Conclusions: In this big real-world data and intermediate-term follow-up study, for patients with diabetes and TVD, PCI and CABG were associated with a lower risk of death and MACCE more than MT. The results suggest the importance of appropriate revascularization for diabetic patients with TVD. However, CABG was not associated with a lower risk of death, but with a lower risk of MACCE, compared with PCI. In the future, we perhaps should strengthen comprehensive treatment in addition to PCI or CABG.
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http://dx.doi.org/10.1186/s12933-020-01193-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7798318PMC
January 2021

Scalable and low-cost fabrication of hydrophobic PVDF/WS porous membrane for highly efficient solar steam generation.

J Colloid Interface Sci 2021 Apr 29;588:369-377. Epub 2020 Dec 29.

School of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, China. Electronic address:

Solar steam generation based on the light-to-heat conversion via photothermal materials has been considered as one of emerged technologies for utilizing solar energy to produce clean water. Here, a hydrophobic PVDF/WS porous membrane for highly efficient solar steam generation was prepared by a scalable and low-cost method. The WS photothermal materials were fabricated through a simple ball milling, and then a non-solvent induced phase inversion method was used to fabricate the porous PVDF/WS membrane. The PVDF/WS evaporator could absorb the sunlight of 90.58% from UV to NIR region due to the multiscattering of the porous structure and the synergistic effect of WS and seawater. Moreover, the PVDF/WS evaporator exhibits the hydrophobic properties. Taking the advantages mentioned above, our evaporator could manifest the evaporation rate of 4.15 kgmh with the solar thermal efficiency of 94.2% under 3 sun irradiation, as well as an outstanding durability upon continuous running. Also, the evaporator shows both the excellent seawater desalination and sewage treatment ability. Outdoor experiments illustrate that the evaporator has practical applications under a natural sunlight condition. The numerous advantages of our PVDF/WS evaporator, including the high solar-thermal efficiency, the outstanding durability, and the simple and scalable manufacture process, may provide a potential photothermal material for the commercial solar desalination application and wastewater treatment.
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http://dx.doi.org/10.1016/j.jcis.2020.12.084DOI Listing
April 2021

circPVT1 promotes osteosarcoma glycolysis and metastasis by sponging miR-423-5p to activate Wnt5a/Ror2 signaling.

Cancer Sci 2021 May 10;112(5):1707-1722. Epub 2021 Mar 10.

Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, China.

Osteosarcoma (OS) is the most prevalent form of bone cancer. It has a high metastatic potential and progresses rapidly. The molecular mechanisms of OS remain unclear and this study aims to examine the functional role of circPVT1 and miR-423-5p in OS. Quantitative RT-PCR (qRT-PCR) and western blotting were used to examine levels of miR-423-5p, circPVT1, Wnt5a, Ror2, and glycolysis-related proteins, including HK2, PKM2, GLUT1, and LDHA. Colony formation and transwell assays were used to test the roles of miR-423-5p, circPVT1, and Wnt5a/Ror2 in OS cell proliferation, migration, and invasion. Dual luciferase assay and Ago2-RIP were used to validate the interactions of miR-423-5p/Wnt5a, miR-423-5p/Ror2, and circPVT1/miR-423-5p. Glucose uptake assay and measurement of lactate production were performed to assess the glycolysis process. A nude mouse xenograft model was used to evaluate the effects of sh-circPVT1 and miR-423-5p mimics on tumor growth and metastasis in vivo. miR-423-5p was reduced in both OS tissues and OS cell lines, while Wnt5a/Ror2 and circPVT1 were elevated. miR-423-5p bound to 3'-UTR of Wnt5a and Ror2 mRNA, and inhibited glycolysis and OS cell proliferation, migration, and invasion by targeting Wnt5a and Ror2. circPVT1 interacted with miR-423-5p and activated Wnt5a/Ror2 signaling by sponging miR-423-5p. Knockdown of circPVT1 or overexpression of miR-423-5p suppressed OS tumor growth and metastasis in vivo. miR-423-5p inhibited OS glycolysis, proliferation, migration, and metastasis by targeting and suppressing Wnt5a/Ror2 signaling pathway, while circPVT1 promoted those processes by acting as a sponge of miR-423-5p.
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http://dx.doi.org/10.1111/cas.14787DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088910PMC
May 2021

β-Catenin/Lin28/let-7 regulatory network determines type II alveolar epithelial stem cell differentiation phenotypes following thoracic irradiation.

J Radiat Res 2021 Jan;62(1):119-132

Department of Oncology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan 450003, China.

The contribution of type II alveolar epithelial stem cells (AEC II) to radiation-induced lung fibrosis (RILF) is largely unknown. Cell differentiation phenotypes are determined by the balance between Lin28 and lethal-7 microRNA (let-7 miRNA). Lin28 is activated by β-catenin. The aim of this study was to track AEC II phenotypes at different phases of injury following thoracic irradiation and examine the expression of β-catenin, Lin28 and let-7 to identify their role in AEC II differentiation. Results showed that coexpression of prosurfactant protein C (proSP-C, an AEC II biomarker) and HOPX (homeobox only protein X, an AEC I biomarker) or vimentin (a differentiation marker) was detected in AEC II post-irradiation. The protein expression levels of HOPX and proSP-C were significantly downregulated, but vimentin was significantly upregulated following irradiation. The expression of E-cadherin, which prevents β-catenin from translocating to the nucleus, was downregulated, and the expression of β-catenin and Lin28 was upregulated after irradiation (P < 0.05 to P < 0.001). Four let-7 miRNA members (a, b, c and d) were upregulated in irradiated lungs (P < 0.05 to P < 0.001), but let-7d was significantly downregulated at 5 and 6 months (P < 0.001). The ratios of Lin28 to four let-7 members were low during the early phase of injury and were slightly higher after 2 months. Intriguingly, the Lin28/let-7d ratio was strikingly increased after 4 months. We concluded that β-catenin contributed to RILF by promoting Lin28 expression, which increased the number of AEC II and the transcription of profibrotic molecules. In this study, the downregulation of let-7d miRNA by Lin28 resulted in the inability of AEC II to differentiate into type I alveolar epithelial cells (AEC I).
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http://dx.doi.org/10.1093/jrr/rraa119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7779353PMC
January 2021

Construction of a mApple-D6A3-mediated biosensor for detection of heavy metal ions.

AMB Express 2020 Dec 7;10(1):213. Epub 2020 Dec 7.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Pollution of heavy metals in agricultural environments is a growing problem to the health of the world's human population. Green, low-cost, and efficient detection methods can help control such pollution. In this study, a protein biosensor, mApple-D6A3, was built from rice-derived Cd-binding protein D6A3 fused with the red fluorescent protein mApple at the N-terminus to detect the contents of heavy metals. Fluorescence intensity of mApple fused with D6A3 indicated the biosensor's sensitivity to metal ions and its intensity was more stable under alkaline conditions. mApple-D6A3 was most sensitive to Cu, then Ni, then Cd. Isothermal titration calorimetry experiments demonstrated that mApple-D6A3 successfully bound to each of these three metal ions, and its ability to bind the ions was, from strongest to weakest, Cu  > Cd  > Ni. There were strong linear relationships between the fluorescence intensity of mApple-D6A3 and concentrations of Cd (0-100 μM), Cu (0-60 μM) and Ni (0-120 μM), and their respective R values were 0.994, 0.973 and 0.973. When mApple-D6A3 was applied to detect concentrations of heavy metal ions in water (0-0.1 mM) or culture medium (0-1 mM), its accuracy for detection attained more than 80%. This study demonstrates the potential of this biosensor as a tool for detection of heavy metal ions.
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http://dx.doi.org/10.1186/s13568-020-01154-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721944PMC
December 2020

Condition-Aware Comparison Scheme for Gait Recognition.

IEEE Trans Image Process 2021 12;30:2734-2744. Epub 2021 Feb 12.

As an important and challenging problem, gait recognition has gained considerable attention. It suffers from confounding conditions, that is, it is sensitive to camera views, dressing types and so on. Interestingly, it is observed that, under different conditions, local body parts contribute differently to recognition performance. In this paper, we propose a condition-aware comparison scheme to measure gait pairs' similarity via a novel module named Instructor. Also, we present a geometry-guided data augmentation approach (Dresser) to enrich dressing conditions. Furthermore, to enhance the gait representation, we propose to model temporal local information from coarse to fine. Our model is evaluated on two popular benchmarks, CASIA-B and OULP. Results show that our method outperforms current state-of-the-art methods, especially in the cross-condition scenario.
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http://dx.doi.org/10.1109/TIP.2020.3039888DOI Listing
July 2021

Multitask Non-Autoregressive Model for Human Motion Prediction.

IEEE Trans Image Process 2021 5;30:2562-2574. Epub 2021 Feb 5.

Human motion prediction, which aims at predicting future human skeletons given the past ones, is a typical sequence-to-sequence problem. Therefore, extensive efforts have been devoted to exploring different RNN-based encoder-decoder architectures. However, by generating target poses conditioned on the previously generated ones, these models are prone to bringing issues such as error accumulation problem. In this paper, we argue that such issue is mainly caused by adopting autoregressive manner. Hence, a novel Non-AuToregressive model (NAT) is proposed with a complete non-autoregressive decoding scheme, as well as a context encoder and a positional encoding module. More specifically, the context encoder embeds the given poses from temporal and spatial perspectives. The frame decoder is responsible for predicting each future pose independently. The positional encoding module injects positional signal into the model to indicate the temporal order. Besides, a multitask training paradigm is presented for both low-level human skeleton prediction and high-level human action recognition, resulting in the considerable improvement for the prediction task. Our approach is evaluated on Human3.6M and CMU-Mocap benchmarks and outperforms state-of-the-art autoregressive methods.
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http://dx.doi.org/10.1109/TIP.2020.3038362DOI Listing
July 2021

Stent-Assisted Coiling of Brain Aneurysms Under Conscious Sedation and Simultaneous Coronary Heart Disease Stenting: A New Concept.

Ann Indian Acad Neurol 2020 Jul-Aug;23(4):572-574. Epub 2019 Jul 11.

Department of Neurosurgery, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.

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http://dx.doi.org/10.4103/aian.AIAN_471_19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7657270PMC
July 2019

Body mass index and mortality in patients with severe coronary artery diseases: A cohort study from China.

Nutr Metab Cardiovasc Dis 2021 02 17;31(2):448-454. Epub 2020 Sep 17.

Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, People's Republic of China; State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, People's Republic of China; National Clinical Research Center for Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, People's Republic of China. Electronic address:

Background And Aims: It is still controversial whether obesity and overweight increase the risk of mortality for patients with coronary artery disease. The current study aimed to investigate the relationship between body mass index (BMI) and mortality in patients with triple-vessel disease (TVD).

Methods And Results: From April 2004 to February 2011, 8943 patients with angiographically confirmed TVD were consecutively enrolled. Patients were divided into five groups according to BMI: underweight (<18.5 kg/m), normal weight (18.5-23.9 kg/m), overweight: (24-27.9 kg/m), mild obesity (28-31.9 kg/m), and severe obesity (≥32 kg/m). The primary end point was all-cause death. Subgroup analysis was performed for treatment strategies: revascularization and medical treatment alone. During a median follow-up of 7.5 years, lower risks of mortality were observed in patients with overweight (adjusted HR 0.85, 95% CI 0.75-0.97) and mild obesity (adjusted HR 0.83, 95% CI 0.69-1.00) compared to those with normal weight. Polynomial Cox regression suggested a U-shape association between BMI and adjusted mortality risk. In the revascularization subgroup, there was a significantly higher mortality risk in patients with severe obesity (adjusted HR 1.57, 95% CI 1.03-2.40) than in those with normal weight. While in the medical treatment subgroup, mortality risk decreased as BMI increased, with the lowest risk being observed in patients with severe obesity.

Conclusion: There is a U-shape relationship between BMI and all-cause death in patients with TVD, with increased risks among both underweight and severely obese patients. This relationship may be influenced by treatment strategies.
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http://dx.doi.org/10.1016/j.numecd.2020.09.011DOI Listing
February 2021

Examining the Relationship of Online Social Networking Sites' Activities, Customers' Brand Choice, and Brand Perception in Health-Related Businesses.

Front Psychol 2020 23;11:546087. Epub 2020 Oct 23.

Department of Commerce, University of Kotli Azad Jammu and Kashmir, Azad Jammu and Kashmir, Pakistan.

The purpose of this study was to examine the effect of online social networking site activities on brand choice for health-related businesses; the study also explored the mediating impact of brand perception on the connection of online social networking site activities and brand choice in health-related businesses. A self-administered questionnaire was used for data collection from 300 customers, randomly selected from health sector businesses in Pakistan. The findings indicate that online social networking site activities had a substantial influence on customers' brand choice in health-related businesses and that brand perception mediates the connection between online social networking site activities and customers' brand choice in health-related businesses. The research also recognized the increasing significance of online social networking sites in health-related businesses. The study offers insights for health-related companies and their managers on visualizing brand perception as well.
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http://dx.doi.org/10.3389/fpsyg.2020.546087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644970PMC
October 2020

Association of and Gene Polymorphisms with Major Adverse Cardiac and Cerebrovascular Events in Patients with Three-Vessel Disease.

Hum Gene Ther 2021 Jun 22;32(11-12):581-588. Epub 2021 Jan 22.

State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Three-vessel disease (TVD) is a severe coronary heart disease (CHD) with poor prognosis. Niemann-Pick C1-like 1 () is a transporter protein for exogenous cholesterol absorption, and 3-hydroxy-3-methylglutaryl-coenzyme A reductase () is a rate-limiting enzyme for cholesterol synthesis. We aimed to investigate the association between and gene polymorphisms and major adverse cardiac and cerebrovascular events (MACCE) in patients with TVD. A total of 342 TVD patients were consecutively enrolled and followed up for 1-year MACCE (a composite of all-cause death, myocardial infarction, revascularization, readmission, and stroke) as TVD event group, and 344 patients without CHD were control group. Four single-nucleotide polymorphisms (SNPs), rs11763759, rs4720470, rs2072183, and rs2073547, on gene and four SNPs, rs12916, rs2303151, rs2303152, and rs4629571, on gene were genotyped. Multivariate logistic regression analysis showed that rs4720470 of was associated with higher risk of TVD with MACCE in codominant model (odds ratio [OR]: 1.315; 95% confidence intervals [CI]: 1.007-1.716,  = 0.044), and that rs2303151 of was associated with higher in recessive (OR: 3.383; 95% CI: 1.040-10.998,  = 0.043) and codominant (OR: 1.458; 95% CI: 1.038-2.047,  = 0.030) model, respectively. Patients with both variant rs4720470 in codominant model and variant rs2303151 in recessive model related to a higher risk (OR: 6.772, CI: 1.338-34.280;  = 0.021). We reported for the first time that the rs4720470 on gene and rs2303151 on gene were associated with risk of 1-year MACCE in TVD.
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http://dx.doi.org/10.1089/hum.2020.229DOI Listing
June 2021
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