Publications by authors named "Jian Sun"

2,159 Publications

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Oscillating field stimulation promotes recovery from spinal cord injury in rats by regulating the differentiation of endogenous neural stem cells.

Exp Ther Med 2021 Sep 12;22(3):979. Epub 2021 Jul 12.

Department of Spine Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230031, P.R. China.

The mammalian spinal cord (SC) has a limited self-repair capacity and exogenous treatments are yet to produce substantial functional recovery following SC injury (SCI). The SC contains endogenous neural stem cells (NSCs) with multi-lineage differentiation potential and it may be possible to restore function via interventions that promote NSC differentiation following SCI. Oscillating field stimulation (OFS) has been reported to regulate the Wnt signaling pathway, a known modulator of NSC differentiation. However, the effects of OFS on NSC differentiation following SCI and associated functional recovery have not been previously examined. In the current study, the Basso-Beattie-Bresnahan (BBB) score was used to assess locomotion recovery following SCI in rats and immunofluorescence double-staining was used to examine the regeneration of neurons and oligodendrocytes derived from NSCs. Furthermore, Nissl staining was performed to assess the viability and survival of neurons following SCI, while recovery of the myelin sheath was examined by uranium-lead staining under transmission electron microscopy. OFS delivered via an implanted stimulator enhanced the differentiation of NSCs into neurons and oligodendrocytes and accelerated the regeneration of myelinated axons. Additionally, BBB scores revealed superior locomotion recovery in OFS-treated rats compared with SCI controls. Collectively, these results indicated that OFS may be a feasible strategy to promote SCI recovery by regulating the differentiation of endogenous NSCs.
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http://dx.doi.org/10.3892/etm.2021.10411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311232PMC
September 2021

An amplitude-based characteristic parameter extraction algorithm for cerebral edema detection based on electromagnetic induction.

Biomed Eng Online 2021 Aug 3;20(1):74. Epub 2021 Aug 3.

College of Biomedical Engineering, Third Military Medical University (Army Medical University), Chongqing, China.

Background: Cerebral edema is a common condition secondary to any type of neurological injury. The early diagnosis and monitoring of cerebral edema is of great importance to improve the prognosis. In this article, a flexible conformal electromagnetic two-coil sensor was employed as the electromagnetic induction sensor, associated with a vector network analyzer (VNA) for signal generation and receiving. Measurement of amplitude data over the frequency range of 1-100 MHz is conducted to evaluate the changes in cerebral edema. We proposed an Amplitude-based Characteristic Parameter Extraction (Ab-CPE) algorithm for multi-frequency characteristic analysis over the frequency range of 1-100 MHz and investigated its performance in electromagnetic induction-based cerebral edema detection and distinction of its acute/chronic phase. Fourteen rabbits were enrolled to establish cerebral edema model and the 24 h real-time monitoring experiments were carried out for algorithm verification.

Results: The proposed Ab-CPE algorithm was able to detect cerebral edema with a sensitivity of 94.1% and specificity of 95.4%. Also, in the early stage, it can detect cerebral edema with a sensitivity of 85.0% and specificity of 87.5%. Moreover, the Ab-CPE algorithm was able to distinguish between acute and chronic phase of cerebral edema with a sensitivity of 85.0% and specificity of 91.0%.

Conclusion: The proposed Ab-CPE algorithm is suitable for multi-frequency characteristic analysis. Combined with this algorithm, the electromagnetic induction method has an excellent performance on the detection and monitoring of cerebral edema.
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http://dx.doi.org/10.1186/s12938-021-00913-4DOI Listing
August 2021

A transposon-associated CRISPR/Cas9 system specifically eliminates both chromosomal and plasmid-borne in .

Antimicrob Agents Chemother 2021 Aug 2:AAC0105421. Epub 2021 Aug 2.

Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, China.

The global spread of antimicrobial-resistant bacteria has been one of the most severe threat to public health. The emergence of gene has posed a considerable threat to antimicrobial medication since it deactivates one last-resort antibiotic, colistin. There have been reports regarding the mobilization of gene facilitated by ISformed transposon Tn and mediated rapid dispersion among species. Here we developed a CRISPR-Cas9 system flanked by IS in a suicide plasmid capable of exerting the sequence-specific curing against bearing plasmid and killing the strain with chromosomal-borne . The constructed IS-carried CRISPR-Cas9 system either restored the sensitivity to colistin of strains with plasmid-borne or directly eradicated the bacteria harbored the chromosomal-borne by introducing an exogenous CRISPR/Cas9 targeting gene. This method is highly efficient in removing gene from and thereby resensitizing these strains to colistin. The further results demonstrated that it conferred the recipient bacteria with the immunity against the acquisition of the exogenous containing the plasmid. The data from the current study highlighted the potential of the transposon-associated CRISPR/Cas9 system to serve as a therapeutic approach to control the dissemination of resistance among clinical pathogens.
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http://dx.doi.org/10.1128/AAC.01054-21DOI Listing
August 2021

Overexpression of phosphatidylserine synthase IbPSS1 affords cellular Na homeostasis and salt tolerance by activating plasma membrane Na/H antiport activity in sweet potato roots.

Hortic Res 2020 Aug 1;7(1):131. Epub 2020 Aug 1.

Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences, Jiangsu Normal University, 221116, Xuzhou, Jiangsu, China.

Phosphatidylserine synthase (PSS)-mediated phosphatidylserine (PS) synthesis is crucial for plant development. However, little is known about the contribution of PSS to Na homeostasis regulation and salt tolerance in plants. Here, we cloned the IbPSS1 gene, which encodes an ortholog of Arabidopsis AtPSS1, from sweet potato (Ipomoea batatas (L.) Lam.). The transient expression of IbPSS1 in Nicotiana benthamiana leaves increased PS abundance. We then established an efficient Agrobacterium rhizogenes-mediated in vivo root transgenic system for sweet potato. Overexpression of IbPSS1 through this system markedly decreased cellular Na accumulation in salinized transgenic roots (TRs) compared with adventitious roots. The overexpression of IbPSS1 enhanced salt-induced Na/H antiport activity and increased plasma membrane (PM) Ca-permeable channel sensitivity to NaCl and HO in the TRs. We confirmed the important role of IbPSS1 in improving salt tolerance in transgenic sweet potato lines obtained from an Agrobacterium tumefaciens-mediated transformation system. Similarly, compared with the wild-type (WT) plants, the transgenic lines presented decreased Na accumulation, enhanced Na exclusion, and increased PM Ca-permeable channel sensitivity to NaCl and HO in the roots. Exogenous application of lysophosphatidylserine triggered similar shifts in Na accumulation and Na and Ca fluxes in the salinized roots of WT. Overall, this study provides an efficient and reliable transgenic method for functional genomic studies of sweet potato. Our results revealed that IbPSS1 contributes to the salt tolerance of sweet potato by enabling Na homeostasis and Na exclusion in the roots, and the latter process is possibly controlled by PS reinforcing Ca signaling in the roots.
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http://dx.doi.org/10.1038/s41438-020-00358-1DOI Listing
August 2020

Hydroxyapatite surface-functionalized monolithic column for selective in-tube solid phase microextraction of zoleronic acid and risedronic acid.

J Chromatogr A 2021 Jul 24;1653:462438. Epub 2021 Jul 24.

College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350108, People's Republic of China.

To date, hydroxyapatite (HAP) based monoliths were mainly fabricated by directly doping of HAP, which suffered from less effective coverage of HAP. Herein, a HAP surface-functionalized monolithic column ([email protected]@UF) has been prepared by in-situ biomineralization and applied as sorbent for selective in-tube solid phase microextraction of zoleronic acid and risedronic acid. A polydopamine coating was first generated on the surface of the parent urea-formaldehyde resin monolith; and then HAP microcrystals were further grew on the polydopamine coating to achieve this preparation. SEM, EDAX, FTIR, XPS and mercury intrusion method were utilized for the characterization of the [email protected]@UF monolith, and provided evidences of this successful preparation. The selective extraction mechanism of the [email protected]@UF monolith was investigated by the optimization of methanol percentage in the sampling solution, phosphate concentration in the eluent. Other crucial factors, including sampling and elution flow rate, and collection time span, were also optimized for the desired SPME performance. Under the optimal conditions, the proposed method showed low LODs of 0.1 μg/mL, satisfactory recoveries of 79.6%-92.5% with RSDs less than 2.7%, and good reproducibility with RSD less than 6.9%, which demonstrated the excellent application of the [email protected]@UF monolith, and its potential as a promising selective sorbent for bisphosphonates.
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http://dx.doi.org/10.1016/j.chroma.2021.462438DOI Listing
July 2021

The negative impact of increasing age and underlying cirrhosis on sensitivity of adenosine deaminase in the diagnosis of tuberculous peritonitis: a cross-sectional study in eastern China.

Int J Infect Dis 2021 Jul 28. Epub 2021 Jul 28.

Department of Infectious Diseases, the First Affiliated Hospital of Wannan Medical College,Wuhu, 241000, China. Electronic address:

Background: This study aims to evaluate the correlation between adenosine deaminase (ADA) test for the diagnosis of tuberculous peritonitis (TBP) and patient age or cirrhosis status.

Methods: Clinical data of patients clinically diagnosed with TPB (n=132) or not (n=147) were assessed. ADA activity was compared among three age groups (< 45 yr, 45-60 yr, and ≥ 60 yr) and cirrhotic-related subgroups. Cut-off values of ADA test were analyzed among three patient populations (young non-cirrhotic, n=97; older non-cirrhotic, n=115; cirrhotic n=67), and validated in a cohort of 259 participants.

Results: By the multivariate regression analyses, age <45 yr is highly predictive of TBP risk. The young non-cirrhotic TBP patients had higher ADA than the middle-aged or old control (P < 0.01). Significantly decreased activity and efficacy of ADA were observed in the cirrhotic subgroup/population, regardless of age or cohort. For the above-mentioned two non-cirrhotic populations in the validation cohort, ADA test showed excellent performance using thresholds of 30.5 IU/L and 20.5 IU/L, with respective sensitivities of 91.1% and 92.6%.

Conclusions: ADA activity is negatively associated with increasing age and underlying cirrhosis. Optimizing cut-off values of ADA test can increase its sensitivity in the non-cirrhotic individuals older than 45 years.
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http://dx.doi.org/10.1016/j.ijid.2021.07.061DOI Listing
July 2021

The antiproliferative activity of ferrocene derivatives against drug-resistant cancer cell lines: A mini review.

Authors:
Li Li Lin Ma Jian Sun

Curr Top Med Chem 2021 Jul 27. Epub 2021 Jul 27.

Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.

Cancer, a highly heterogeneous disease at intra/inter patient levels, remains a serious health problem contributing to significant morbidity and mortality worldwide. Despite great progress in clinical treatment, the concerns impeding the success of conventional cancer chemotherapy is descending efficacy of anticancer agents due to the development of drug resistance especially multiple drug resistance (MDR). Ferrocene derivatives have a different mode of action to the platinum anticancer drugs, and the ferrocene-phenol hybrid ferrocifen exhibits potential activity against drug-resistant cancers. Currently, ferrocifen is in preclinical trial, demonstrating that ferrocene derivatives are useful scaffolds for the development of novel anticancer candidates which are active against drug-resistant cancers. In the present review, the current scenario of ferrocene derivatives including ferrocene metal complexes, hybrids and other derivatives with antiproliferative potential against drug-resistant cancer cell lines is summarized for further rational design.
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http://dx.doi.org/10.2174/1568026621666210728093527DOI Listing
July 2021

Inflammatory Myofibroblastic Tumors in Children: A Clinical Retrospective Study on 19 Cases.

Front Pediatr 2021 8;9:543078. Epub 2021 Jul 8.

Department of Cardiothoracic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, China.

Inflammatory myofibroblastic tumor (IMFT) is a rare neoplasm mainly affecting children and young adults. We conducted a retrospective study to evaluate the clinical features and treatment alternatives of childhood inflammatory myofibroblastic tumors. A total of 19 patients who were pathologically diagnosed with IMT between December 2008 and October 2018 were included. Collected data were demographic information, main complaints, tumor characteristics, treatment, pathological results, immunohistochemical analysis, and prognosis. The male/female ratio was 13:6. The mean age at disease onset was 44.9 ± 33.9 months (range 4 to 111 months). The mean tumor size was 6.5 ± 4.0 cm (range 1.2 to 17.0 cm). The most common site was the abdomen (13/19). The most commonly used detection tool was CT. Eleven patients (57.9%) had aggressive tumor growth, including eight receiving extensive resection and three receiving palliative resection due to high local invasiveness and postoperative chemotherapy. Eight cases whose tumors were completely enveloped received complete resection. Immunohistochemistry was performed for 17 patients and ALK positivity was found in 11 patients. Despite three children lost to follow-up, sixteen patients were followed up for 6 to 132 months (average 63.9 months, median 66 months). Of which, twelve children survived with no evidence of IMT, and four cases (21%) showed local recurrences (two of them died). No distant metastasis was detected. IMT is rare in children with various locations, mostly appearing in the abdomen. Whether the tumor could be completely removed, the location and the invasiveness of surrounding tissues might be highly prognosis-related.
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http://dx.doi.org/10.3389/fped.2021.543078DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295553PMC
July 2021

Rehabilitation therapy outside of the hospital via the Internet combined with suggestive psychological intervention in patients with trigeminal neuralgia.

Am J Transl Res 2021 15;13(6):6837-6845. Epub 2021 Jun 15.

Department of Neurology, Zhenjiang Fourth People's Hospital Zhenjiang 212000, Jiangsu, China.

Objective: To explore the effect of rehabilitation therapy outside of the hospital via the Internet combined with suggestive psychological intervention on the mental state, sleep quality, self-rehabilitation, and psychological resilience of patients with trigeminal neuralgia.

Methods: A total of 90 patients with trigeminal neuralgia in our hospital from May 2018 to December 2019 were selected and divided into a control group and an observation group according to different nursing methods. The control group (n=45) was given the conventional nursing intervention, and the observation group (n=45) was given the rehabilitation therapy outside of the hospital via the Internet combined with suggestive psychological intervention. The mental state, sleep quality, self-rehabilitation, psychological resilience and compliance rate of the two groups before and after care were compared.

Results: After care, the SAS and SDS scores of the observation group were lower than those of the control group (P<0.05). After care, the VAS score and PSQI score of the observation group were lower than those of the control group (P<0.05). After care, the scores of self-care responsibility, self-concept, self-care skills, and health knowledge in the ES-CA scales of two groups and the total score were significantly increased, and the observation group was higher than the control group (P<0.05). After care, the scores of optimism, self-improvement, and resilience in the CD-RISC scales of the two groups and their total scores were significantly increased, and the observation group was higher than the control group (P<0.05). The compliance rate of the observation group was 95.23%, which was significantly higher than 76.19% of the control group (P<0.05).

Conclusion: Rehabilitation therapy outside of the hospital combined with suggestive psychological intervention via Internet is a promising method for trigeminal neuralgia recovery.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290665PMC
June 2021

Comparative multi-omics analysis of hypoxic germination tolerance in weedy rice embryos and coleoptiles.

Genomics 2021 Jul 20. Epub 2021 Jul 20.

Rice Research Institute, Shenyang Agricultural University, Shenyang 110161, China. Electronic address:

Hypoxic germination tolerance is an important trait for seedling establishment of direct-seeded rice. Our comparative metabolomics analysis revealed that weedy rice accumulated more sugar and amino acids than cultivated rice accumulated in the embryo and coleoptile tissues under hypoxic stress. At the transcriptional level, oxidative phosphorylation activity in weedy rice was higher than in cultivated rice that likely led to more efficient energy metabolism during hypoxic stress. Based on our comparative proteomics analysis, enriched proteins related to cell wall implied that the advantages in energy metabolism of weedy rice were ultimately reflected in the formation of tissue structures. In this study, we found that most of key hypoxic germination tolerance (HGT) genes shared the same genetic backgrounds with Oryza japonica, however, several of them genetically similar to other Oryza plant also play important roles. Our findings suggest weedy rice can serve as genetic resources for the improvement of direct-seeding rice.
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http://dx.doi.org/10.1016/j.ygeno.2021.07.021DOI Listing
July 2021

The Emergence and Molecular Characteristics of New Delhi Metallo -Lactamase-Producing From Ducks in Guangdong, China.

Front Microbiol 2021 5;12:677633. Epub 2021 Jul 5.

National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China.

This study aimed to determine the prevalence and transmission characteristics of New Delhi metallo β-lactamase (NDM)-producing from ducks in Guangdong, China. In this study, a total of 28 NDM-producing isolates were recovered from 88 unduplicated diseased duck samples (31.8%) from veterinary clinics in Guangzhou, Foshan, Qingyuan, and Huizhou. Two variants, and , were detected and the latter was present in 89.6% of the isolates (25/28). Multilocus sequence typing (MLST) analysis indicated that these isolates possessed six distinct STs, and ST156 was the most prevalent followed by ST648, ST746, ST354, ST10, and ST162. In addition, phylogenomic analysis found that two of the isolates that were recovered from a single sample possessed different genomes, and the -carrying IncX3 plasmids may be horizontal transfer between isolates in the intestinal tracts of ducks. Whole-genome sequencing (WGS) analysis further revealed that co-existed with other 25 types of antimicrobial resistance genes (ARGs), of which 16 ARGs were highly prevalent with detection rates >50%, and a high incidence of coproducing and isolates (22/88, 25.0%) was detected in ducks. This study underscores the importance of surveillance for -harboring microbes in ducks.
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http://dx.doi.org/10.3389/fmicb.2021.677633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287858PMC
July 2021

Litoribacter populi sp. nov., isolated from the soil of a Populus euphratica forest.

Arch Microbiol 2021 Jul 19. Epub 2021 Jul 19.

Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Xinjiang Laboratory of Special Environmental Microbiology, Urumqi, 830091, Xinjiang, China.

A Gram-stain-negative, rod-shaped, and orange-pigmented bacterium, designated XAAS-A1, was isolated from the soil of a Populus euphratica forest. The main respiratory quinone of XAAS-A1 is MK-7, and the principal cellular fatty acids are iso-C, C ω6c, Iso-C 3OH, and summed feature 3 (Cω6c and/or Cω7c). The major polar lipids are phosphatidylethanolamine, phosphatidylcholine, and two unidentified lipids. The 16S rRNA gene sequence analysis indicated that XAAS-A1 belongs to the genus Litoribacter within the family Cyclobacteriaceae, having the highest sequence similarities to Litoribacter ruber KCTC 22899 (97.1%) and L. alkaliphilus KCTC 32217 (96.9%). XAAS-A1 has a DNA G + C content of 42.1 mol%. The orthoANI and the digital DNA-DNA hybridization values were markedly lower than the recommended threshold values of 96% and 70%. Phenotypic and genotypic data suggest that XAAS-A1 represents a novel species of the genus Litoribacter, for which we propose the name Litoribacter populi sp. nov. and XAAS-A1 (= CCTCC AB 2017159 = KCTC 62045) as the type strain.
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http://dx.doi.org/10.1007/s00203-021-02488-zDOI Listing
July 2021

Loss of nocturnal dipping pattern of skin sympathetic nerve activity during and following an extended-duration work shift in residents in training.

J Cardiol 2021 Jul 16. Epub 2021 Jul 16.

Department of Cardiology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, 1665 Kongjiang Road, Shanghai 200092, China. Electronic address:

Background: Extended-duration work shifts (EDWSs) might affect the health of physician residents, causing autonomic alteration. Skin sympathetic nerve activity (SKNA) recorded by noninvasive neuro-electrocardiography (neuECG) is used to estimate cardiac sympathetic tone. In this study, we aim to evaluate the impact of EDWSs on nocturnal SKNA assessed in resident doctors.

Methods: Twenty-four residents working EDWSs and 12 PhD students not working nightshift schedules were prospectively recruited. The neuECG was performed between 12 am and 6 am for 5 consecutive nights. SKNA was filtered from neuECG recorded signals. The questionnaires regarding work stress and sleep quality, blood pressure, and salivary alpha-amylase and cortisol levels were administered.

Results: The hours of weekly working and sleep opportunities were similar between residents and students, while residents reported more work stress and worse sleep quality. In residents, SKNA at 6 am (SKNA) was significantly higher than SKNA during the precall night, revealing a dipping pattern. However, the SKNA dipping disappeared during the on-call night and prominently flattened during the first postcall night, the full recovery of which was delayed until the second postcall nights. The morning blood pressure and salivary alpha-amylase and cortisol levels were similar between the precall and postcall days. In contrast, SKNA in students exhibited a constant dipping profile for all recorded nights.

Conclusions: In healthy young adults, SKNA presents a dip night. The SKNA dip is impaired by working a nightshift, with a delayed recovery. The neuECG might serve as a useful tool to detect subclinical autonomic disturbances in shiftworkers.
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http://dx.doi.org/10.1016/j.jjcc.2021.06.006DOI Listing
July 2021

Serum hepatitis B virus RNA level is associated with biochemical relapse in patients with chronic hepatitis B infection who discontinue nucleos(t)ide analogue treatment.

Aliment Pharmacol Ther 2021 Jul 18. Epub 2021 Jul 18.

State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: Nucleos(t)ide analogue (NA) discontinuation may be attempted in carefully selected patients with chronic hepatitis B (CHB) infection.

Aim: To investigate whether a novel serum marker of quantitative hepatitis B virus (HBV) RNA levels could predict biochemical relapse after NA discontinuation.

Methods: We prospepctively followed non-cirrhotic Asian patients with CHB who stopped NA according to pre-specified stopping criteria. The primary endpoint was biochemical relapse (HBV DNA >2000 IU/mL and alanine transaminase >2x upper limit of normal), which were also the re-treatment criteria.

Results: Biochemical relapse occurred in 50 patients (48.3% at year 6). Multivariable analysis showed that higher HBV RNA levels (HR 1.34; P < 0.001) at the time of NA discontinuation were associated with increased biochemical relapse risk. The area under the curve of HBV RNA at the time of NA discontinuation for the incidence of biochemical relapse was 0.760 at 6 years. Six years after treatment discontinuation, all patients with HBV RNA levels ≥20 000 copies/mL at the end of treatment developed a biochemical relapse compared with 23.8% of patients with HBV RNA levels<1000 copies/mL (P < 0.001). More patients with HBV RNA levels <1000 copies/mL at end of treatment achieved loss of hepatitis B surface antigen than patients with higher levels (30.9% vs 1.6%; P = 0.027).

Conclusions: The HBV RNA level at end of treatment predicted biochemical relapse after treatment discontinuation and may be used to guide decisions on treatment discontinuation.
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http://dx.doi.org/10.1111/apt.16538DOI Listing
July 2021

Diagnostic value of multi-slice spiral CT (MSCT) combined with digestive tract angiography in gastric fundus cardia carcinoma.

J BUON 2021 May-Jun;26(3):911-916

Department of Medical Imaging, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, China.

Purpose: To evaluate the diagnostic value of multi-slice spiral CT (MSCT) combined with digestive tract angiography in patients with gastric fundus cardia carcinoma.

Methods: A total of 185 patients with suspected gastric fundus cardia carcinoma admitted in our hospital were collected. Among them, 93 patients were examined with MSCT combined with digestive tract angiography and were enrolled in the research group. Another 92 patients examined by MSCT alone comprised the control group. The diagnostic value of MSCT combined with digestive tract angiography in patients with gastric fundus cardia carcinoma was investigated. 185 patients were diagnosed by pathological examination and 166 had gastric fundus cardia carcinoma, with 84 patients in the research group, and 84 patients in the control group. Fifty nine patients with gastric fundus cardia carcinoma in the control group were diagnosed by MSCT.

Results: There were significant differences compared with pathological diagnosis (p<0.05). Eighty-two patients with gastric fundus cardia carcinoma in the research group were diagnosed by MSCT combined with digestive tract angiography. There were no significant differences compared with pathological diagnosis (p>0.05). Sensitivity, specificity and accuracy of the research group were significantly higher than those of the control group (p<0.05). The detectable rate in imaging results of the research group was higher than that of the control group (p<0.05).

Conclusion: MSCT combined with digestive tract angiography is more accurate than single MSCT in the diagnosis of gastric fundus cardia carcinoma, which can effectively reduce the misdiagnosis and missed diagnosis and is worthy of clinical promotion.
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July 2021

Inhaled Corticosteroids and the Pneumonia Risk in Patients With Chronic Obstructive Pulmonary Disease: A Meta-analysis of Randomized Controlled Trials.

Front Pharmacol 2021 29;12:691621. Epub 2021 Jun 29.

Department of Respiratory and Critical Care Medicine, Chengdu Second People's Hospital, Chengdu, China.

Whether all types of inhaled corticosteroids (ICSs) would increase the pneumonia risk in patients with chronic obstructive pulmonary disease (COPD) remains controversial. We aimed to assess the association between ICSs treatment and pneumonia risk in COPD patients, and the impact of medication details and baseline characteristics of patients on the association. Four databases (PubMed, Embase, Cochrane Library, and Clinical Trials.gov) were searched to identify eligible randomized controlled trials (RCTs) comparing ICSs treatment with non-ICSs treatment on the pneumonia risk in COPD patients. Pooled results were calculated using Peto odds ratios (Peto ORs) with corresponding 95% confidence intervals (CIs). A total of 59 RCTs enrolling 103,477 patients were analyzed. All types of ICSs significantly increased the pneumonia risk (Peto OR, 1.43; 95% CI, 1.34-1.53). Subgroup analysis showed that there was a dose-response relationship between ICSs treatment and pneumonia risk (low-dose: Peto OR, 1.33; 95% CI, 1.22-1.45; medium-dose: Peto OR, 1.50; 95% CI, 1.28-1.76; and high-dose: Peto OR, 1.64; 95% CI, 1.45-1.85). Subgroup analyses based on treatment durations and baseline characteristics (severity, age, and body mass index) of patients were consistant with the above results. Subgroup analysis based on severity of pneumonia showed that fluticasone (Peto OR, 1.75; 95% CI, 1.44-2.14) increased the risk of serious pneumonia, while budesonide and beclomethasone did not. ICSs treatment significantly increased the risk of pneumonia in COPD patients. There was a dose-response relationship between ICSs treatment and pneumonia risk. The pneumonia risk was related with COPD severity.
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http://dx.doi.org/10.3389/fphar.2021.691621DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275837PMC
June 2021

IS Is Responsible for the Evolution and Transmission of -Harboring Plasmids in Escherichia coli of Poultry Origin in China.

mSystems 2021 Jul 13:e0064621. Epub 2021 Jul 13.

Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

Carbapenem-resistant Enterobacteriaceae are some of the most important pathogens responsible for nosocomial infections, which can be challenging to treat. The carbapenemase genes, which are expressed by New Delhi metallo-β-lactamase (NDM)-producing Escherichia coli isolates, have been found in humans, environmental samples, and multiple other sources worldwide. Importantly, these genes have also been found in farm animals, which are considered an NDM reservoir and an important source of human infections. However, the dynamic evolution of genetic contexts and -harboring plasmids has not been directly observed, making it difficult to assess the extent of horizontal dissemination of the gene. In this study, we detected NDM-1 ( = 1), NDM-5 ( = 24), and NDM-9 ( = 8) variants expressed by E. coli strains isolated from poultry in China from 2016 to 2017. By analyzing the immediate genetic environment of the genes, we found that IS was associated with multiple types of multidrug resistance regions, and we identified various IS-derived circular intermediates. Importantly, in E. coli strain GD33, we propose that IncHI2 and IncI1 plasmids can fuse when IS is present. Our analysis of the IS elements flanking allowed us to propose an important role for IS elements in the evolution of multidrug-resistant regions (MRRs) and in the dissemination of . To the best of our knowledge, this is the first description of the dynamic evolution of genetic contexts and -harboring plasmids. These findings could help proactively limit the transmission of these NDM-producing isolates from food animals to humans. Carbapenem resistance in members of the order Enterobacterales is a growing public health problem that is associated with high mortality in developing and industrialized countries. Moreover, in the field of veterinary medicine, the occurrence of New Delhi metallo-β-lactamase-producing Escherichia coli isolates in animals, especially food-producing animals, has become a growing concern in recent years. The wide dissemination of is closely related to mobile genetic elements (MGEs) and plasmids. Although previous analyses have explored the association of many different MGEs with mobilization of , little is known about the evolution of various genetic contexts of in E. coli. Here, we report the important role of IS in forming multiple types of multidrug resistance cassettes and the dynamic recombination of plasmids bearing . These results suggest that significant attention should be paid to monitoring the transmission and further evolution of -harboring plasmids among E. coli strains of food animal origin.
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http://dx.doi.org/10.1128/mSystems.00646-21DOI Listing
July 2021

Longitudinal monitor of Jun N-terminal kinase pathway associated phosphatase reflects clinical efficacy to triple conventional disease-modifying anti-rheumatic drugs treatment in rheumatoid arthritis patients.

Inflammopharmacology 2021 Aug 12;29(4):1131-1138. Epub 2021 Jul 12.

Department of Rheumatology and Immunology, Affiliated Hospital of Jiangnan University, No. 200 Huihe Road, Binhu District, Wuxi, 214062, China.

Objective: This study aimed to investigate the correlation of Jun N-terminal kinase pathway associated phosphatase (JKAP) with inflammation, disease activity, and clinical efficacy to triple conventional disease-modifying anti-rheumatic drugs (cDMARDs) in rheumatoid arthritis (RA) patients.

Methods: A total of 119 active RA patients about to receive triple cDMARDs treatment were enrolled. Serum JKAP was detected by enzyme-linked immunosorbent assay at week0, week6, week12, and week24 (W24). According to clinical response status or remission status at W24, RA patients were classified as response patients and non-response patients, or remission patients and non-remission patients, respectively.

Results: JKAP was negatively correlated with erythrocyte sedimentation rate (ESR), C-reactive protein, and 28-joints disease activity score based on ESR (DAS28 score (ESR)), while JKAP was not correlated with disease duration, tender joint count, swollen joint count, health assessment questionnaire for rheumatoid arthritis or treatment history. Furthermore, during 24-week triple cDMARDs treatment, JKAP was increased overtime. Subgroup analyses showed that JKAP displayed a rising trend in response patients, remission patients, non-remission patients but not non-response patients, meanwhile its increment was more obvious in remission patients versus non-remission patients. Additionally, JKAP at W24 was higher in response patients compared with non-response patients, and JKAP at W12 and W24 was higher in remission patients compared with non-remission patients.

Conclusion: Longitudinal monitor of JKAP might reflect clinical efficacy to the treatment of triple cDMARDs, which could improve outcomes in RA patients.
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http://dx.doi.org/10.1007/s10787-021-00823-wDOI Listing
August 2021

Flame-made Cu/ZrO catalysts with metastable phase and strengthened interactions for CO hydrogenation to methanol.

Chem Commun (Camb) 2021 Jul;57(61):7509-7512

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China.

Facile synthesis of a highly efficient Cu/ZrO2 catalyst by a flame spray pyrolysis (FSP) method was developed for methanol production from CO2 hydrogenation. The instantaneous quenching process from extremely high temperature in FSP was used for rational design of a Cu/ZrO2 catalyst, not only producing the metastable tetragonal phase ZrO2, but also strengthening the metal-support interaction. The methanol yield of one-step synthesized FSP-Cu/ZrO2 was 3 times higher than the one made by the traditional method. This strategy is anticipated to pave the way for strengthening metal-support interactions of supported catalysts.
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http://dx.doi.org/10.1039/d1cc02784kDOI Listing
July 2021

Security-Based Passivity Analysis of Markov Jump Systems via Asynchronous Triggering Control.

IEEE Trans Cybern 2021 Jul 8;PP. Epub 2021 Jul 8.

This article considers the security-based passivity problem for a class of discrete-time Markov jump systems in the presence of deception attacks, where the deception attacks aim to change the transmitted signal. Considering the impact of deception attacks on network disruption, it causes the existence of time-varying delays in signal transmission inevitably, which makes the controlled system and the controller work asynchronously. The asynchronous control method is employed to overcome the nonsynchronous phenomenon between the system mode and controller mode. On the other hand, to reduce the frequency of data transmission, a resilient asynchronous event-triggered control scheme taking deception attacks into account is designed to save communication resources, and the proposed controller can cover some existing ones as special examples. Moreover, different triggering conditions corresponding to different jumping modes are developed to decide whether state signals should be transferred. A new stability criterion is derived to ensure the passivity of the resultant system although there exist deception attacks. Finally, a simulation example is given to verify the theoretical analysis.
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http://dx.doi.org/10.1109/TCYB.2021.3090398DOI Listing
July 2021

ATF6-DGAT pathway is involved in TLR7-induced innate immune response in Ctenopharyngodon idellus kidney cells.

Dev Comp Immunol 2021 Nov 3;124:104197. Epub 2021 Jul 3.

College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China. Electronic address:

DGAT1 and DGAT2 are two acyl-CoA:diacylglycerol O-acyltransferase (DGAT) enzymes that catalyze the final step in triglyceride (TG) synthesis. TGs are the primary constituents of lipid droplets (LDs). Although it has been demonstrated that LDs modulate immune and inflammatory responses in CIK cells, little is known about whether DGAT1 and DGAT2 involve in this process. Firstly, grass carp DGAT2 was isolated and characterized, encoding 361 amino acids, and all DGAT2 proteins in genomic structures are conserved in vertebrates. Then, using TLR7 agonist, we induced LDs accumulation in CIK cells. Only DGAT1b and DGAT2 were upregulated in forming TLR7 agonist induced-LDs. Next, we utilized small-molecule inhibitors of DGAT1 and DGAT2. The results indicated that DGAT1 inactivation attenuated TG content and the relative expressions of IFNα3, NF-κB, IL-1β, and TNFα genes, whereas DGAT2 inhibition decreased TG content and the relative expressions of MyD88, IRF7, IFNα3, NF-κB, IL-1β, and TNFα genes, implying that DGAT1-generated LDs and DGAT2-generated LDs contribute to TLR7-induced immune response via different signaling pathways. Finally, inhibiting ATF6 effectively decreased DGAT-generated LDs accumulation and the expression of TLR7 signaling-related genes induced by TLR7 agonist, implying that ATF6 UPR pathway may mediate the role of DGAT-generated LDs in TLR7 signaling. Overall, we demonstrate that DGAT1 and DGAT2-catalyzed TAG synthesis may generate different LDs to provide distinct signaling platforms for innate TLR7 signaling.
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http://dx.doi.org/10.1016/j.dci.2021.104197DOI Listing
November 2021

Analysis of Continuous Prevalence Survey of Healthcare-Associated Infections Based on the Real-Time Monitoring System in 2018 in Shandong in China.

Biomed Res Int 2021 11;2021:6693889. Epub 2021 Jun 11.

Department of Infection Control, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China.

Background: Healthcare-associated infection (HAI) is a serious threat to the safety of patients worldwide. The prevalence survey is widely used to explore and study the characteristics of HAI. However, the annual continuous prevalence survey of hospital-acquired infections has not been reported so far.

Aim: This study is aimed at examining the occurrence and development trend of HAIs dynamically and accurately.

Methods: An annual continuous HAI prevalence survey based on the real-time monitoring system was conducted in representative hospitals from different regions in Shandong in China. . A total of 64 hospitals participated in the survey, and 2,741,433 patients were monitored in 2018. The highest prevalence of HAIs in Shandong was 3.83% (February 15), the lowest was 1.85% (February 28), and the average was 2.45%. The percentile distribution of prevalence of HAIs in this study was as follows: P10, 2.23%; P25, 2.31%; P50, 2.41%; P75, 2.55%; and P90, 2.73%.

Conclusion: This study dynamically and accurately showed the occurrence and development trend of HAIs in Shandong in 2018. The results of this study can be used as a reference for the HAI prevalence survey in various medical institutions in Shandong and provide the basis for the regional HAI prevention and control strategy.
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http://dx.doi.org/10.1155/2021/6693889DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213461PMC
June 2021

[email protected] activated peroxymonosulfate system for effectively degrading emerging contaminants: Analysis of the formation and activation mechanism of Fe coordinately unsaturated metal sites.

J Hazard Mater 2021 Jun 29;419:126535. Epub 2021 Jun 29.

Institute of Environmental Research at Greater Bay Area, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, China.

Carbon-encapsulated Fe nanocomposites ([email protected]), obtained by pyrolysis of metal-organic frameworks (MOFs), can activate peroxymonosulfate (PMS) to remove emerging contaminants (ECs). Unfortunately, the current MOFs-derived catalysts always inevitably produce more iron-oxide compounds that unfavorable for PMS activation. In this work, according to the thermogravimetric curve of Fe(II)-MOF-74, to discuss the role of pyrolysis temperature on the structural characteristics of [email protected] The results demonstrated that [email protected] could obtain abundant coordinately unsaturated metal sites and exhibited the best activation performance. Radical-quenching experiments and EPR measurements confirm that the generated sulfate radical (SO˙) and singlet oxygen (O) only degraded approximately 35% of TBBPA. Meanwhile, negatively charged complex intermediates formed by the weak interaction between [email protected] and PMS was proposed as the dominant reactive species, and approximately 65% of TBBPA was degraded. This work optimizes the synthesis strategy and mechanism of [email protected] and provides a methodological reference for the design of Fe-based catalysts.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126535DOI Listing
June 2021

Hepatocyte-derived L-carnitine restricts hepatitis B surface antigen loss through an immunosuppressive effect on germinal center-related immune cells.

J Infect Dis 2021 Jul 2. Epub 2021 Jul 2.

State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: The outcome of hepatitis B virus (HBV) infection is significantly affected by host immune response; herein, we aim to dissect the effect of L-carnitine (L-Cn) on germinal center (GC)-related immune cells and the influence on the prognosis of HBV infection.

Methods: In vitro and in vivo experiments were performed in patients with chronic HBV infection and a hydrodynamic injection mouse model.

Results: In vitro assays revealed that L-Cn significantly reduced GC-related immune responses and enhanced immunosuppressive profiles. Intriguingly, L-Cn released from lysed hepatocytes was associated with the degree of liver damage. Besides, the administration of L-Cn in an HBV mouse model resulted in delayed clearance of HBsAg in serum and decreased GC formation in the spleen. Notably, patients with HBsAg loss showed decreased plasma L-Cn levels, and longitudinal observations found that low baseline levels of L-Cn were associated with a favorable treatment response in chronic hepatitis B patients.

Conclusions: The suppressive effect of hepatocyte-derived L-Cn on GC-related immune cells may contribute to the inability of HBsAg clearance in chronic HBV infection, indicating that L-Cn might serve as a potential therapeutic target for the treatment of HBV infection.
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http://dx.doi.org/10.1093/infdis/jiab344DOI Listing
July 2021

Learning Polynomial-Based Separable Convolution for 3D Point Cloud Analysis.

Authors:
Ruixuan Yu Jian Sun

Sensors (Basel) 2021 Jun 19;21(12). Epub 2021 Jun 19.

School of Mathematics and Statistics, Xi'an Jiaotong University, Xi'an 710049, China.

Shape classification and segmentation of point cloud data are two of the most demanding tasks in photogrammetry and remote sensing applications, which aim to recognize object categories or point labels. Point convolution is an essential operation when designing a network on point clouds for these tasks, which helps to explore 3D local points for feature learning. In this paper, we propose a novel point convolution (PSConv) using separable weights learned with polynomials for 3D point cloud analysis. Specifically, we generalize the traditional convolution defined on the regular data to a 3D point cloud by learning the point convolution kernels based on the polynomials of transformed local point coordinates. We further propose a separable assumption on the convolution kernels to reduce the parameter size and computational cost for our point convolution. Using this novel point convolution, a hierarchical network (PSNet) defined on the point cloud is proposed for 3D shape analysis tasks such as 3D shape classification and segmentation. Experiments are conducted on standard datasets, including synthetic and real scanned ones, and our PSNet achieves state-of-the-art accuracies for shape classification, as well as competitive results for shape segmentation compared with previous methods.
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http://dx.doi.org/10.3390/s21124211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235014PMC
June 2021

Molecular Epidemiology of Fosfomycin Resistant from a Pigeon Farm in China.

Antibiotics (Basel) 2021 Jun 25;10(7). Epub 2021 Jun 25.

National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

We determined the prevalence and molecular characteristics of fosfomycin-resistant from a domestic pigeon farm. A total of 79 samples collected from pigeons and their surrounding environments were screened for the presence of fosfomycin resistant isolates and these included 49 isolates that displayed high-level resistance (MIC ≥ 256 mg L) and carried the gene on plasmids with sizes ranging from 80 to 370 kb. MLST analysis of these -positive isolates indicated the presence of nine sequence types (ST6856, ST8804, ST457, ST746, ST533, ST165, ST2614, ST362 and ST8805) of which ST6856 was the most prevalent (24.5%, 12/49). PFGE combined with genomic context comparative analyses indicated that the gene was spread by horizontal transfer as well as via clonal transmission between in the pigeon farm, and IS played an important role in transmission. The high prevalence of in the pigeon farm and the high similarity of the genomic environment between isolates from humans and pigeons indicated that the pigeon farm served as a potential reservoir for human infections. The pigeon farm was found to be an important reservoir for the gene and this should be further monitored.
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http://dx.doi.org/10.3390/antibiotics10070777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300711PMC
June 2021

Trends and Urban-Rural Disparities of Energy Intake and Macronutrient Composition among Chinese Children: Findings from the China Health and Nutrition Survey (1991 to 2015).

Nutrients 2021 Jun 4;13(6). Epub 2021 Jun 4.

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

The nutrition status of children is gaining more attention with a rapid nutrition transition. This study aimed to investigate trends and urban-rural differences in dietary energy and macronutrient composition among Chinese children. A total of 7565 participants aged 6 to 17 years were obtained from three rounds (1991, 2004 and 2015) of the Chinese Health and Nutrition Survey (CHNS). The individual diet was evaluated via three consecutive 24-hour dietary recalls and compared with the Chinese Dietary Reference Intakes (DRIs). From 1991 to 2015, there was a significant increase in children's fat intake, the proportion of energy intake from fat, and the proportion of children with more than 30% of energy from fat and less than 50% of energy from carbohydrates ( < 0.001). Compared with the DRI, the proportion with higher fat and lower carbohydrate intakes were, respectively, 64.7% and 46.8% in 2015. The urban-rural disparities in fat and carbohydrate intake gradually narrowed, while the gap in protein intake increased notably over time ( < 0.001). Chinese children experienced a rapid transformation to a low-carbohydrate and high-fat diet. Urban-rural disparities persistently existed; further nutritional interventions and education were of great significance, so as to ensure a more balanced diet for Chinese children.
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http://dx.doi.org/10.3390/nu13061933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229111PMC
June 2021

The contribution of Urban and Rural Resident Basic Medical Insurance to income-related inequality in depression among middle-aged and older adults: Evidence from China.

J Affect Disord 2021 Jun 20;293:168-175. Epub 2021 Jun 20.

Institute of Health Policy, Management and Evaluation, University of Toronto, Toronto, Canada.

Objective: Previous studies have not investigated the contribution of medical insurance to income-related inequality in depressive symptoms. To fulfill this research gap, this study aimed to assess the contribution of Urban and Rural Resident Basic Medical Insurance (URRBMI) to income-related inequality in depressive symptoms among middle-aged and older adults in China.

Methods: The data of this study was obtained from the 2018 wave of China Health and Retirement Longitudinal Study (CHARLS). The data of Particulate Matter 2.5 (PM) concentrations were sourced from Atmospheric Composition Analysis Group. Furthermore, concentration curve and concentration index were employed to measure the extent of income-related inequality in depressive symptoms. Moreover, decomposition method of concentration index was used to quantify the contribution of URRBMI to the income-related inequality in depressive symptoms.

Results: The concentration index values of depression occurrence and score were -0.1067 and -0.0712, respectively, indicating pro-rich inequality. The decomposition results reveal that the contribution rate of URRBMI to concentration index of depression occurrence was 18.88%, which indicates that it reduced the pro-rich inequality in depression occurrence. In addition, the contribution rate of URRBMI to concentration index of depression score was 3.55%, indicating that it relieved the pro-rich inequality in depression score.

Conclusion: This study found pro-rich inequalities in depression occurrence and score which were reduced with the coverage of URRBMI. It is quite necessary to further expand the coverage of URRBMI.
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http://dx.doi.org/10.1016/j.jad.2021.06.027DOI Listing
June 2021

Investigation and Analysis of Sharp Injuries among Health Care Workers from 36 Hospitals in Shandong Province, China.

Biomed Res Int 2021 11;2021:5698483. Epub 2021 Jun 11.

Department of Infection Control, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China.

Background: This study investigated and analyzed the current situation of sharp injuries among health care workers (HCWs) in China's Shandong Province.

Methods: By means of questionnaire survey, the incidence of sharp injuries among HCWs from 36 hospitals in China's Shandong Province in October 2019 was investigated, and the results of this survey were compared with those of October 2012.

Results: A total of 48165 HCWs were investigated. 549 cases of sharp injuries occurred. The incidence of sharp injuries was 1.14%, which was significantly lower than that in October 2012 (9.71%). In the occupational distribution of sharp injuries among HCWs, the proportion of nurses was 58.65%, doctors 23.32%, and interns 12.02%. Among the distribution of sharp injury departments, general wards, operating rooms, intensive care units, disinfection supply centers, and outpatient clinics were the high-incidence sites of occupational exposure among HCWs. The main instruments causing sharp injuries in HCWs were syringes, scalp steel needles, surgical suture needles, vacuum blood collection needles, and glass slides. Drug administration, double-handed loop needle cap, blood extraction, surgical suture needle, and arteriovenous needle extraction were high-risk operations causing sharp instrument injuries in HCWs.

Conclusion: The incidence of sharp injuries among HCWs from 36 hospitals in Shandong Province in October 2019 was significantly lower than that in October 2012. Sharp injuries were a common type of occupational exposure for HCWs. The occurrence of sharp injuries should be effectively reduced by changing wrong habitual behavior and implementing standard protective measures.
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http://dx.doi.org/10.1155/2021/5698483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214496PMC
June 2021

Exosomal microRNA-16-5p from adipose mesenchymal stem cells promotes TLR4-mediated M2 macrophage polarization in septic lung injury.

Int Immunopharmacol 2021 Jun 27;98:107835. Epub 2021 Jun 27.

Department of Critical Care Medicine, The Second Hospital of Jilin University, Changchun 130021, Jilin Provence, China. Electronic address:

Objective: Reports on septic lung injury have implicated the protective effects of adipose mesenchymal stem cells-derived Exos (ADSCs-Exos). At present, we tried to explain the mechanism of ADSCs-Exos-loaded microRNA (miR)-16-5p in inflammatory response and macrophage polarization by regulating Toll-like Receptor 4 (TLR4).

Methods: ADSCs-Exos were obtained and transfected with miR-16-5p agomir. The septic lung injury model was induced by cecal ligation and puncture and the modeled mice were injected with the Exos or silenced TLR4 vector, after which inflammation, pathological changes, macrophage polarization in the lung tissue were monitored. In vitro RAW246.7 macrophages were stimulated by lipopolysaccharide (LPS), interfered with Exos or TLR4-related vector and detected for inflammatory response.

Results: ADSCs-Exos reduced lung injury in septic mice and promoted M2 polarization. Up-regulating exosomal miR-16-5p inhibited macrophage inflammation and relieved lung injury in septic mice. Silencing TLR4 improved lung inflammation while overexpression of TLR4 impaired the impact of exosomal miR-16-5p in septic mice. Restoration of TLR4 reduced the impact of exosomal miR-16-5p on LPS-treated RAW 264.7 macrophages.

Conclusion: Exosomal miR-16-5p from ADSCs promotes macrophage polarization and attenuates septic lung injury in mice via suppressing TLR4.
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http://dx.doi.org/10.1016/j.intimp.2021.107835DOI Listing
June 2021
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