Publications by authors named "Jian Qin"

464 Publications

Effects of the association between APOE rs405509 polymorphisms and gene-environment interactions on hand grip strength among middle-aged and elderly people in a rural population in southern China.

J Orthop Surg Res 2021 Jun 11;16(1):372. Epub 2021 Jun 11.

School of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region, China.

Background: Hand grip strength is a complex phenotype. The current study aimed to identify the effects of the association between APOE rs405509 polymorphisms and gene-environment interactions on hand grip strength among middle-aged and elderly people in a rural population in Gongcheng, southern China.

Methods: APOE rs405509 polymorphisms in 1724 participants (695 men and 1029 women, aged 45-97 years old) were genotyped using the Sequenom MassARRAY platform. Statistical analysis was conducted using SPSS 21.0 and Plink 1.90.

Results: The APOE rs405509 G allele was associated with lower hand grip strength in all participants (β = -1.04, P value <0.001), and the correlation seemed to be even stronger among women. A significant gene-environment interaction was observed between APOE rs405509 and smoking, especially in men. The hand grip strength of male smokers carrying the GG genotype was significantly higher than that of nonsmokers (P value = 0.004).

Conclusions: APOE rs405509 polymorphisms might be genetic factors that affect hand grip strength in a rural population in Gongcheng, southern China. The APOE rs405509-smoking interaction has an impact on hand grip strength.
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http://dx.doi.org/10.1186/s13018-021-02522-2DOI Listing
June 2021

Smoke-Free Home Rules and Association with Child Secondhand Smoke Exposure among Mother-Child Dyad Relationships.

Int J Environ Res Public Health 2021 May 14;18(10). Epub 2021 May 14.

Department of Health Behavior & Policy, Virginia Commonwealth University, Richmond, VA 23284, USA.

Smoke-free home rules restrict smoking in the home, but biomarkers of secondhand smoke exposure are needed to help understand the association between smoke-free homes and child secondhand smoke exposure. Participants ( = 346) were majority Black/African American mother-child dyads from a longitudinal study in North Carolina. Mothers completed questionnaires on household smoking behaviors and rules, and child saliva samples were assayed for secondhand smoke exposure. Regression models used smoke-free home rules to predict child risk for secondhand smoke exposure. Children in households with smoke-free home rules had less salivary cotinine and risk for secondhand smoke exposure. After controlling for smokers in the household, home smoking rules were not a significant predictor of secondhand smoke exposure. Compared to children in households with no smokers, children in households with at least one smoker but a non-smoking mother (OR 5.35, 95% CI: 2.22, 13.17) and households with at least one smoker including a smoking mother (OR 13.73, 95% CI: 6.06, 33.28) had greater risk for secondhand smoke exposure. Results suggest smoke-free home rules are not sufficient to fully protect children from secondhand smoke exposure, especially in homes with smokers. Future research should focus on how household members who smoke can facilitate the prevention of child secondhand smoke exposure.
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http://dx.doi.org/10.3390/ijerph18105256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157188PMC
May 2021

An Intelligent Self-Service Vending System for Smart Retail.

Sensors (Basel) 2021 May 20;21(10). Epub 2021 May 20.

Department of Electrical Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

The traditional weighing and selling process of non-barcode items requires manual service, which not only consumes manpower and material resources but is also more prone to errors or omissions of data. This paper proposes an intelligent self-service vending system embedded with a single camera to detect multiple products in real-time performance without any labels, and the system realizes the integration of weighing, identification, and online settlement in the process of non-barcode items. The system includes a self-service vending device and a multi-device data management platform. The flexible configuration of the structure gives the system the possibility of identifying fruits from multiple angles. The height of the system can be adjusted to provide self-service for people of different heights; then, deep learning skill is applied implementing product detection, and real-time multi-object detection technology is utilized in the image-based checkout system. In addition, on the multi-device data management platform, the information docking between embedded devices, WeChat applets, Alipay, and the database platform can be implemented. We conducted experiments to verify the accuracy of the measurement. The experimental results demonstrate that the correlation coefficient R2 between the measured value of the weight and the actual value is 0.99, and the accuracy of non-barcode item prediction is 93.73%. In Yangpu District, Shanghai, a comprehensive application scenario experiment was also conducted, proving that our system can effectively deal with the challenges of various sales situations.
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http://dx.doi.org/10.3390/s21103560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161222PMC
May 2021

Influence of manganese exposure on cognitive function, plasma APP and Aβ levels in older men.

J Trace Elem Med Biol 2021 May 14;67:126788. Epub 2021 May 14.

Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning, 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning, 530021, Guangxi, China. Electronic address:

Background: Elevated manganese (Mn) exposure impairs cognition in adults and children, but the association between Mn and cognitive function in elderly people is unclear. Previous studies have linked Mn neurotoxicity in AD to Aβ-dependent mechanisms. However, the association between Mn and plasma APP and Aβ in the general elderly population remains unknown. This study aimed to investigate the association between Mn exposure and cognitive function, plasma APP and plasma Aβ in older adults.

Methods: Cognitive abilities in 375 men aged 60 and older in Guangxi, China were assessed using the Mini-Mental State Examination (MMSE) and cognitive impairment were identified using education-stratified cut-off points of MMSE scores. Urinary Mn levels and plasma APP, and Aβ levels were measured using ICP-MS and ELISA, respectively.

Results: A total of 109 (29.07 %) older men were identified as having cognitive impairment. The median urinary Mn level was 0.22 μg/g creatinine. Urinary Mn levels were negatively correlated with MMSE scores (β = -1.35, 95 % CI: -2.65 to -0.06; p = 0.041). In addition, higher concentrations of urinary manganese were associated with a greater risk of cognitive impairment (OR = 2.03, 95 % CI: 1.14-3.59; comparing the highest and lowest manganese; p = 0.025). Moreover, plasma APP levels were inversely associated with urinary Mn levels (r = -0.123, p = 0.020), and positively associated with MMSE scores (r = 0.158, p = 0.002). Surprisingly, no correlations were observed between plasma Aβ42, Aβ40, Aβ40/Aβ42, or Aβ42/Aβ40 and urinary Mn levels and MMSE scores.

Conclusion: These results suggested that Mn exposure is negatively associated with older men's cognition and plasma APP levels, but not plasma Aβ levels.
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http://dx.doi.org/10.1016/j.jtemb.2021.126788DOI Listing
May 2021

Dual-Solvent Li-Ion Solvation Enables High-Performance Li-Metal Batteries.

Adv Mater 2021 May 9:e2008619. Epub 2021 May 9.

Department of Materials Science and Engineering, Stanford University, Stanford, CA, 94305, USA.

Novel electrolyte designs to further enhance the lithium (Li) metal battery cyclability are highly desirable. Here, fluorinated 1,6-dimethoxyhexane (FDMH) is designed and synthesized as the solvent molecule to promote electrolyte stability with its prolonged -CF - backbone. Meanwhile, 1,2-dimethoxyethane is used as a co-solvent to enable higher ionic conductivity and much reduced interfacial resistance. Combining the dual-solvent system with 1 m lithium bis(fluorosulfonyl)imide (LiFSI), high Li-metal Coulombic efficiency (99.5%) and oxidative stability (6 V) are achieved. Using this electrolyte, 20 µm Li||NMC batteries are able to retain ≈80% capacity after 250 cycles and Cu||NMC anode-free pouch cells last 120 cycles with 75% capacity retention under ≈2.1 µL mAh lean electrolyte conditions. Such high performances are attributed to the anion-derived solid-electrolyte interphase, originating from the coordination of Li-ions to the highly stable FDMH and multiple anions in their solvation environments. This work demonstrates a new electrolyte design strategy that enables high-performance Li-metal batteries with multisolvent Li-ion solvation with rationally optimized molecular structure and ratio.
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http://dx.doi.org/10.1002/adma.202008619DOI Listing
May 2021

Protective effects of calorie restriction on insulin resistance and islet function in STZ-induced type 2 diabetes rats.

Nutr Metab (Lond) 2021 May 5;18(1):48. Epub 2021 May 5.

Department of Traditional Chinese Medicine, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen, 518107, China.

Background: Caloric restriction (CR) has become increasingly attractive in the treatment of type 2 diabetes mellitus (T2DM) because of the increasingly common high-calorie diet and sedentary lifestyle. This study aimed to evaluate the role of CR in T2DM treatment and further explore its potential molecular mechanisms.

Methods: Sixty male Sprague-Dawley rats were used in this study. The diabetes model was induced by 8 weeks of high-fat diet (HFD) followed by a single dose of streptozotocin injection (30 mg/kg). Subsequently, the diabetic rats were fed HFD at 28 g/day (diabetic control) or 20 g/day (30% CR regimen) for 20 weeks. Meanwhile, normal rats fed a free standard chow diet served as the vehicle control. Body mass, plasma glucose levels, and lipid profiles were monitored. After diabetes-related functional tests were performed, the rats were sacrificed at 10 and 20 weeks, and glucose uptake in fresh muscle was determined. In addition, western blotting and immunofluorescence were used to detect alterations in AKT/AS160/GLUT4 signaling.

Results: We found that 30% CR significantly attenuated hyperglycemia and dyslipidemia, leading to alleviation of glucolipotoxicity and thus protection of islet function. Insulin resistance was also markedly ameliorated, as indicated by notably improved insulin tolerance and homeostatic model assessment for insulin resistance (HOMA-IR). However, the improvement in glucose uptake in skeletal muscle was not significant. The upregulation of AKT/AS160/GLUT4 signaling in muscle induced by 30% CR also attenuated gradually over time. Interestingly, the consecutive decrease in AKT/AS160/GLUT4 signaling in white adipose tissue was significantly reversed by 30% CR.

Conclusion: CR (30%) could protect islet function from hyperglycemia and dyslipidemia, and improve insulin resistance. The mechanism by which these effects occurred is likely related to the upregulation of AKT/AS160/GLUT4 signaling.
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http://dx.doi.org/10.1186/s12986-021-00575-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097947PMC
May 2021

-inositol improves growth performance and regulates lipid metabolism of juvenile Chinese mitten crab () fed different percentage of lipid.

Br J Nutr 2021 Apr 29:1-13. Epub 2021 Apr 29.

Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of Life Sciences, East China Normal University, Shanghai200241, People's Republic of China.

This study evaluated the effects of dietary myo-inositol (MI) on growth performance, antioxidant status and lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis) fed different percentage of lipid. Crabs (4·58 (sem 0·05) g) were fed four diets including a normal lipid diet (N, containing 7 % lipid and 0 mg/kg MI), N with MI supplementation (N + MI, containing 7 % lipid and 1600 mg/kg MI), a high lipid diet (H, containing 13 % lipid and 0 mg/kg MI) and H with MI supplementation (H + MI, containing 13 % lipid and 1600 mg/kg MI) for 8 weeks. The H + MI group showed higher weight gain and specific growth rate than those in the H group. The dietary MI could improve the lipid accumulations in the whole body, hepatopancreas and muscle as a result of feeding on the high dietary lipid (13 %) in crabs. Besides, the crabs fed the H + MI diets increased the activities of antioxidant enzymes but reduced the malondialdehyde content in hepatopancreas compared with those fed the H diets. Moreover, dietary MI enhanced the expression of genes involved in lipid oxidation and exportation, yet reduced lipid absorption and synthesis genes expression in the hepatopancreas of crabs fed the H diet, which might be related to the activation of inositol 1,4,5-trisphosphate receptor (IP3R)/calmodulin-dependent protein kinase kinase-β (CaMKKβ)/adenosine 5'-monophosphate-activated protein kinase (AMPK) signalling pathway. This study demonstrates that MI could increase lipid utilisation and reduce lipid deposition in the hepatopancreas of E. sinensis fed a high lipid diet through IP3R/CaMKKβ/AMPK activation. This work provides new insights into the function of MI in the diet of crustaceans.
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http://dx.doi.org/10.1017/S0007114521001409DOI Listing
April 2021

Heralded Nondestructive Quantum Entangling Gate with Single-Photon Sources.

Phys Rev Lett 2021 Apr;126(14):140501

Hefei National Laboratory for Physical Sciences at Microscale and Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026, China.

Heralded entangling quantum gates are an essential element for the implementation of large-scale optical quantum computation. Yet, the experimental demonstration of genuine heralded entangling gates with free-flying output photons in linear optical system, was hindered by the intrinsically probabilistic source and double-pair emission in parametric down-conversion. Here, by using an on-demand single-photon source based on a semiconductor quantum dot embedded in a micropillar cavity, we demonstrate a heralded controlled-NOT (CNOT) operation between two single photons for the first time. To characterize the performance of the CNOT gate, we estimate its average quantum gate fidelity of (87.8±1.2)%. As an application, we generated event-ready Bell states with a fidelity of (83.4±2.4)%. Our results are an important step towards the development of photon-photon quantum logic gates.
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http://dx.doi.org/10.1103/PhysRevLett.126.140501DOI Listing
April 2021

Extract of inhibits lipid accumulation and ameliorates HFD-induced obesity in mice through regulating adipose differentiation by decreasing PPARγ and CEBP/α expression.

Food Nutr Res 2021 1;65. Epub 2021 Mar 1.

Tomas Lindahl Nobel Laureate Laboratory, Precision Medicine Research Centre, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, Guangdong, China.

Background: Obesity is a principal risk factor for the development of type 2 diabetes and cardiovascular diseases. Natural plants and/or foods play an important role in the management of obesity. (AAL) is a kind of potherb popular among Asian populations, and it is also consumed as a food ingredient and traditional herbal medicine.

Objective: We investigated the effects of water extract from AAL on high-fat-diet (HFD)-induced obese mice and 3T3-L1 adipocytes to develop a new functional food material.

Design: Nine-week-old male mice were randomly divided into control (chow diet, = 6) and HFD ( = 30) group. From 12-weeks onward, mice in the HFD group were further separated into model (saline, 6 mL/kg), simvastatin (0.11 mg/mL, 6 mL/kg), and AAL treatment (low, middle, and high dosage: 300, 600, and 900 mg/kg) group, with 6 animals per group, while mice in the control group were treated with saline (6 mL/kg). Food intake, body/fat weight, liver/kidney indexes, and lipid profiles were determined. Tissues were fixed with formalin for pathological examination. Western blotting and PCR were performed to evaluate the protein and mRNA expression in 3T3-L1 adipocytes. Oil Red O staining was used to determine lipid accumulation.

Results: AAL administration significantly suppressed body weight gain, and reduced fat pad weight and Lee's index in obese mice, but had no effect on liver/kidney index. AAL also reduced serum cholesterol, triglyceride, and LDL-C and increased HDL-C levels. Histological analysis revealed that AAL significantly ameliorated lipid accumulation in the liver and subcutaneous adipose tissue. , Oil Red O staining showed that AAL inhibited adipose differentiation by down-regulating the gene and protein expression of PPARγ and C/EBPα. AAL also reversed HFD-induced intestinal dysbacteriosis.

Conclusion: AAL water-soluble extract has a significant anti-adipogenic effect in the HFD-induced obese mice model.
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http://dx.doi.org/10.29219/fnr.v65.424DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955518PMC
March 2021

Effects of Gene and Plasma Tau on Cognitive Impairment in Rural Chinese Population.

Curr Alzheimer Res 2021 Mar 24. Epub 2021 Mar 24.

Department of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, No.22, Shuangyong Road, Nanning530021. China.

Background: Sufficient attention was not paid to the effects of microtubule-associated protein tau (MAPT) and plasma tau protein on cognition.

Objective: A total of 3072 people in rural China were recruited. They were provided with question- naires, and blood samples were obtained.

Methods: The MMSE score was used to divide the population into cognitive impairment group and control group. First, logistic regression analysis was used to explore the possible factors influenc- ing cognitive function. Second, 1837 samples were selected for SNP detection through stratified sampling. Third, 288 samples were selected to test three plasma biomarkers (tau, phosphorylated tau, and Aβ-42).

Results: For the MAPT rs242557, people with AG genotypes were 1.32 times more likely to devel- op cognitive impairment than those with AA genotypes, and people with GG genotypes were 1.47 times more likely to develop cognitive impairment than those with AG phenotypes. The plasma tau protein concentration was also increased in the population carrying G (P = 0.020). The plasma tau protein was negatively correlated with the MMSE score (P = 0.004).

Conclusion: The mutation of MAPT rs242557 (A > G) increased the risk of cognitive impairment and the concentration of plasma tau protein.
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http://dx.doi.org/10.2174/1567205018666210324122840DOI Listing
March 2021

Electrochemically Regulated Li Deposition by Crown Ether.

ACS Appl Mater Interfaces 2021 Apr 24;13(13):15872-15880. Epub 2021 Mar 24.

Hubei Key Lab of Electrochemical Power Sources, College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072, China.

The lithium-secondary battery is considered to be the most prospective electrochemical energy storage in the upcoming decades. However, its real application still much depends on the effective strategy toward Li dendrite growth. After years of effort, many successful works have been reported on improving the solid-electrolyte interphase (SEI), either via electrolyte optimization or building artificial SEI while intrinsically adjusting the electrochemical reduction of Li has been rarely mentioned. Inspired by the successful works in the electroplating industry, in this paper, a Li-chelating agent, benzo-15-crown-5 (B15C5) was used to regulate Li-reduction kinetics from an electrochemical view. Owing to the coordination with Li, Li + complex + e → Li[complex] is generated and proved by a decreased value. B15C5 confined within the PVC matrix has been coated on a Li anode. With thus-obtained B15C5-PVC-Li, dendrite growth has been significantly reduced and prolonged cycling has been observed in Li|Li symmetric cells. Electrochemically modulated Li deposition has been further accessed by the full cell of LiFePO|Li, and 163 mA h/g capacity is stably released after 400 cycles at 1.0 mA/cm. This study provides an alternate approach to address the dendrite growth issue and sheds more light on the Li-deposition kinetics.
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http://dx.doi.org/10.1021/acsami.1c01476DOI Listing
April 2021

Influences of dietary vitamin D on growth, antioxidant capacity, immunity and molting of Chinese mitten crab (Eriocheir sinensis) larvae.

J Steroid Biochem Mol Biol 2021 Jun 3;210:105862. Epub 2021 Mar 3.

Laboratory of Aquaculture Nutrition and Environmental Health, School of Life Sciences, East China Normal University, Shanghai, 200241, PR China. Electronic address:

This study investigates the effects of vitamin D (VD) on growth performance, antioxidant capacity, immunity and molting of larval Chinese mitten crab Eriocheir sinensis. A total of 6,000 larvae (7.52 ± 0.10 mg) were fed with six isonitrogenous and isolipidic experimental diets with different levels of dietary VD (0, 3000, 6000, 9000, 12000 and 36000 IU/kg) respectively for 23 days. The highest survival and molting frequency were found in crabs fed 6000 IU/kg VD. Weight gain, specific growth rate, and carapace growth significantly increased in crabs fed 3000 and 6000 IU/kg VD compared to the control. Broken-line analysis of molting frequency, weight gain and specific growth rate against dietary VD levels indicates that the optimal VD requirement for larval crabs is 4825-5918 IU/kg. The highest whole-body VD content occurred in the 12000 IU/kg VD group, and the 25-dihydroxy VD content decreased with the increase of dietary VD. The malonaldehyde content was lower than the control. Moreover, the superoxide dismutase activity, glutathione peroxidase and total antioxidant capacity of crab fed 6000 IU/kg VD were significantly higher than in control. Crabs fed 9000 IU/kg showed the highest survival after 120 h of salinity stress, and the relative mRNA expressions indicate vitamin D receptor (VDR) is the important regulatory element in molting and innate immunity. The molting-related gene expressions showed that the response of crab to salinity was self-protective. This study would contribute to a new understanding of the molecular basis underlying molting and innate immunity regulation by vitamin D in E. sinensis.
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http://dx.doi.org/10.1016/j.jsbmb.2021.105862DOI Listing
June 2021

Nitrated Polycyclic Aromatic Hydrocarbons and Arachidonic Acid Metabolisms Relevant to Cardiovascular Pathophysiology: Findings from a Panel Study in Healthy Adults.

Environ Sci Technol 2021 03 23;55(6):3867-3875. Epub 2021 Feb 23.

Nicholas School of the Environment, Duke University, Durham, North Carolina 27708, United States.

Concerns on nitrated polycyclic aromatic hydrocarbons (nitro-PAHs) in the environment have mainly arisen from their mutagenic and carcinogenic effects. The objective of this study is to investigate whether nitro-PAH exposures are associated with biomarkers of cardiovascular pathophysiology. In a panel study design, urines and blood samples were collected up to four times with a 2-week interval from 89 healthy adults. We measured 1-naphthylamine, 2-naphthylamine, 9-aminophenanthrene, 2-aminofluorene, and 1-aminopyrene as biomarkers of nitro-PAH exposures. We measured three urinary metabolites of arachidonic acid (AA) including 20-hydroxyeicosatetraenoic acid (20-HETE) from the cytochrome P450 (CYP) pathway, 8-isoprostane from the nonenzymatic pathway, and 11-dehydro-thromboxane B2 (11-dhTXB) from the cyclooxygenase (COX) pathway. Urinary malondialdehyde, 8-hydroxy-2'-deoxyguanosine (8-OHdG), and 6-sulfatoxymelatonin (aMT6s) were measured to reflect systemic oxidative stress. Plasma concentrations of the soluble P-selectin and von Willebrand factor (vWF) were measured as biomarkers of platelet activation and endothelial dysfunction. We found that increased urinary concentrations of amino-PAHs were significantly associated with increased 20-HETE, 11-dhTXB, and 8-OHdG and with decreased 8-isoprostane and aMT6s. Increased amino-PAHs were positively associated with P-selectin and vWF, respectively. These results suggest that exposure to nitro-PAHs increases systemic oxidative stress and alters AA metabolism toward CYP and COX pathways, leading to an increased cardiovascular disease risk.
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http://dx.doi.org/10.1021/acs.est.0c08150DOI Listing
March 2021

Physical networks from entropy-driven non-covalent interactions.

Nat Commun 2021 Feb 2;12(1):746. Epub 2021 Feb 2.

Department of Materials Science & Engineering, Stanford University, Stanford, CA, USA.

Physical networks typically employ enthalpy-dominated crosslinking interactions that become more dynamic at elevated temperatures, leading to network softening. Moreover, standard mathematical frameworks such as time-temperature superposition assume network softening and faster dynamics at elevated temperatures. Yet, deriving a mathematical framework connecting the crosslinking thermodynamics to the temperature-dependent viscoelasticity of physical networks suggests the possibility for entropy-driven crosslinking interactions to provide alternative temperature dependencies. This framework illustrates that temperature negligibly affects crosslink density in reported systems, but drastically influences crosslink dynamics. While the dissociation rate of enthalpy-driven crosslinks is accelerated at elevated temperatures, the dissociation rate of entropy-driven crosslinks is negligibly affected or even slowed under these conditions. Here we report an entropy-driven physical network based on polymer-nanoparticle interactions that exhibits mechanical properties that are invariant with temperature. These studies provide a foundation for designing and characterizing entropy-driven physical crosslinking motifs and demonstrate how these physical networks access thermal properties that are not observed in current physical networks.
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http://dx.doi.org/10.1038/s41467-021-21024-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854746PMC
February 2021

Changes in the regional homogeneity of resting-state magnetic resonance imaging in perimenopausal women.

BMC Womens Health 2021 01 28;21(1):39. Epub 2021 Jan 28.

Department of Radiology, The Second Affiliated Hospital of Shandong First Medical University, No.366 Taishan Street, Tai'an, Shandong, China.

Background: There is a noticeable lack of systematic researches on evaluating the correlation between serum estrogen levels and changes in brain functional areas of perimenopausal women.The aim of this study is to investigate the regional spontaneous brain activity changes in perimenopausal women.

Methods: Based on the resting-state functional magnetic resonance imaging datasets acquired from 25 perimenopausal women and 20 healthy women of reproductive age, a two-sample t-test was performed on individual normalized regional homogeneity (ReHo) maps. Relationships between abnormal ReHo values and the self-rating anxiety scale (SAS), the self-rating depression scale (SDS) were investigated with Pearson correlation analysis. We also investigated the correlation between abnormal ReHo values and serum estrogen level.

Results: In the perimenopausal group, we found increased ReHo in the right posterior cerebellum (region 2), left middle frontal gyrus and left middle cingulate gyrus ([Formula: see text]). Additionally, the ReHo values in left middle frontal gyrus and leftt middle cingulate gyrus showed positively significant correlation with the SAS, SDS scores. On the contrary, there was no significant correlation between the ReHo value in right posterior cerebellum and SDS, SAS scores. In the perimenopausal group, the ReHo values in the left middle frontal gyrus and left middle cingulate gyrus were negatively correlated with the serum estrogen level ([Formula: see text]).

Conclusion: The results of this preliminary study have suggested that abnormal spontaneous activities of multiple brain regions during resting state was already altered in perimenopausal women. Alterative activities might be related to emotional regulation deficits and cognitive impairment, and might potentially represent the neural mechanism underlying perimenopausal period.
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http://dx.doi.org/10.1186/s12905-020-01171-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842067PMC
January 2021

A CNN-Based Length-Aware Cascade Road Damage Detection Approach.

Sensors (Basel) 2021 Jan 20;21(3). Epub 2021 Jan 20.

Guangzhou Electronic Technology Co., Ltd., Chinese Academy of Sciences, Guangzhou 510070, China.

Accurate and robust detection of road damage is essential for public transportation safety. Currently, deep convolutional neural networks (CNNs)-based road damage detection algorithms to localize and classify damage with a bounding box have achieved remarkable progress. However, research in this field fails to take into account two key characteristics of road damage: weak semantic information and abnormal geometric properties, resulting in inappropriate feature representation and suboptimal detection results. To boost the performance, we propose a CNN-based cascaded damage detection network, called CrdNet. The proposed model has three parts: (1) We introduce a novel backbone network, named LrNet, that reuses low-level features and mixes suitable range dependency features to learn high-to-low level feature fusions for road damage weak semantic information representation. (2) We apply multi-scale and multiple aspect ratios anchor mechanism to generate high-quality positive samples regarding the damage with abnormal geometric properties for network training. (3) We designed an adaptive proposal assignment strategy and performed cascade predictions on corresponding branches that can establish different range dependencies. The experiments show that the proposed method achieves mean average precision (mAP) of 90.92% on a collected road damage dataset, demonstrating the good performance and robustness of the model.
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http://dx.doi.org/10.3390/s21030689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864040PMC
January 2021

Coniferaldehyde prevents articular cartilage destruction in a murine model via Nrf2/HO‑1 pathway.

Mol Med Rep 2021 03 26;23(3). Epub 2021 Jan 26.

Department of Orthopaedics, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu 211100, P.R. China.

Osteoarthritis (OA) is the most prevalent joint disorder characterized by progressive cartilage damage, resulting in gradual disability among the elderly. We previously provided evidence that nuclear factor erythroid 2‑related factor 2 (Nrf2) deficiency is associated with the development of OA. It has been reported that coniferaldehyde (CFA) acts as a potential Nrf2 activator. The aim of the present study was to investigate the protective effects of CFA against osteoarthritis. A murine model of surgical‑induced OA was used in the present study and CFA was administered by peritoneal injection every day, and the knee joints were assessed by histological analysis. The results demonstrated that CFA activated the Nrf2 signaling pathway in primary chondrocytes and articular cartilage from the knee joints. Cartilage damage in mice subjected to the destabilization of the medial meniscus was evidently alleviated by CFA treatment. CFA also robustly suppressed apoptosis induced by H2O2 in murine chondrocytes and reduced the expression of matrix metalloproteinase (MMP)1, MMP3, interleukin (IL)‑1 and IL‑6 in vivo. On the whole, the findings suggested that CFA exerts a therapeutic effect against OA, and the activation of the Nrf2/heme oxygenase‑1 pathway may play a crucial role in CFA‑mediated cartilage protection.
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http://dx.doi.org/10.3892/mmr.2021.11863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851827PMC
March 2021

Ergosterol limits osteoarthritis development and progression through activation of Nrf2 signaling.

Exp Ther Med 2021 Mar 8;21(3):194. Epub 2021 Jan 8.

Department of Orthopaedics, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu 211100, P.R. China.

Osteoarthritis (OA) is a common joint disorder characterized by progressive articular cartilage degeneration and destruction and results in gradual disability among middle-aged and elderly patients. Our previous study demonstrated that depletion of nuclear factor erythroid 2-related factor 2 (Nrf2) exacerbated cartilage erosion in an OA model and that activation of the Nrf2 pathway could counter this process. As a downstream target of Nrf2, heme oxygenase (HO) degrades heme to free iron, biliverdin and carbon monoxide (CO), which protects against oxidative stress. Ergosterol (ER), which is extracted from fungi, is a newly discovered Nrf2 activator and displayed efficacy against myocardial injury. The present study aimed to investigate the potential protective effects of ER against cartilage damage during OA. Primary mouse chondrocytes were treated with ER for assays. Furthermore, mice that underwent destabilization of the medial meniscus surgery were orally administered with ER. Western blotting suggested that ER increased protein expression of Nrf2 and HO-1 in primary chondrocytes and articular cartilage from knee joints. Cartilage damage in knee joints was significantly reduced by ER treatment. Western blotting and PCR analysis confirmed that ER could also suppress the expression of MMP-9 and MMP-13 and . The present findings suggested that ER effectively alleviated cartilage degradation and that activation of the Nrf2-heme oxygenase 1 pathway may play a role in ER-mediated cartilage protection against OA.
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http://dx.doi.org/10.3892/etm.2021.9627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812583PMC
March 2021

Long intergenic non-coding RNA LINC00485 exerts tumor-suppressive activity by regulating miR-581/EDEM1 axis in colorectal cancer.

Aging (Albany NY) 2021 01 10;13(3):3866-3885. Epub 2021 Jan 10.

School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China.

Long non-coding RNAs (lncRNA) play a vital role in colorectal cancer (CRC) progression. To investigate the role of long intergenic non-coding RNA in CRC, we performed functional experiments. LoVo tumor-bearing and liver metastasis mice were used as models. We found that expression was significantly lower in CRC tissues and cancer cells than in paired normal samples and human normal colonic epithelial cells. Lower expression of predicted poor prognosis in CRC patients. knockdown promoted the proliferation, migration, and invasion of FHC cells, while overexpression weakened these abilities of LoVo cells. MicroRNA was the downstream target of , which was downregulated in CRC samples and cancer cells compared to normal tissues and normal colonic epithelial cells. overexpression induced proliferation, migration, and invasion of FHC cells, while antagomir treatment produced opposite results. directly targeted the 3'UTR of and inhibited its expression and induction of epithelial-mesenchymal transition of CRC. In mouse models, knockdown or down-regulation of significantly repressed CRC cell growth and prevented CRC liver metastasis. Overall, suppressed CRC tumorigenesis and progression by targeting the / axis. may be a potential therapeutic target for CRC.
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http://dx.doi.org/10.18632/aging.202354DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906134PMC
January 2021

Brain parcellation driven by dynamic functional connectivity better capture intrinsic network dynamics.

Hum Brain Mapp 2021 Apr 7;42(5):1416-1433. Epub 2020 Dec 7.

College of Intelligence Science and Technology, National University of Defense Technology, Changsha, Hunan, China.

Until now, dynamic functional connectivity (dFC) based on functional magnetic resonance imaging is typically estimated on a set of predefined regions of interest (ROIs) derived from an anatomical or static functional atlas which follows an implicit assumption of functional homogeneity within ROIs underlying temporal fluctuation of functional coupling, potentially leading to biases or underestimation of brain network dynamics. Here, we presented a novel computational method based on dynamic functional connectivity degree (dFCD) to derive meaningful brain parcellations that can capture functional homogeneous regions in temporal variance of functional connectivity. Several spatially distributed but functionally meaningful areas that are well consistent with known intrinsic connectivity networks were identified through independent component analysis (ICA) of time-varying dFCD maps. Furthermore, a systematical comparison with commonly used brain atlases, including the Anatomical Automatic Labeling template, static ICA-driven parcellation and random parcellation, demonstrated that the ROI-definition strategy based on the proposed dFC-driven parcellation could better capture the interindividual variability in dFC and predict observed individual cognitive performance (e.g., fluid intelligence, cognitive flexibility, and sustained attention) based on chronnectome. Together, our findings shed new light on the functional organization of resting brains at the timescale of seconds and emphasized the significance of a dFC-driven and voxel-wise functional homogeneous parcellation for network dynamics analyses in neuroscience.
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http://dx.doi.org/10.1002/hbm.25303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927310PMC
April 2021

Quantum computational advantage using photons.

Science 2020 12 3;370(6523):1460-1463. Epub 2020 Dec 3.

Hefei National Laboratory for Physical Sciences at Microscale and Department of Modern Physics, University of Science and Technology of China, Hefei, Anhui 230026, China.

Quantum computers promise to perform certain tasks that are believed to be intractable to classical computers. Boson sampling is such a task and is considered a strong candidate to demonstrate the quantum computational advantage. We performed Gaussian boson sampling by sending 50 indistinguishable single-mode squeezed states into a 100-mode ultralow-loss interferometer with full connectivity and random matrix-the whole optical setup is phase-locked-and sampling the output using 100 high-efficiency single-photon detectors. The obtained samples were validated against plausible hypotheses exploiting thermal states, distinguishable photons, and uniform distribution. The photonic quantum computer, , generates up to 76 output photon clicks, which yields an output state-space dimension of 10 and a sampling rate that is faster than using the state-of-the-art simulation strategy and supercomputers by a factor of ~10.
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http://dx.doi.org/10.1126/science.abe8770DOI Listing
December 2020

Growth, Metabolite, Antioxidative Capacity, Transcriptome, and the Metabolome Response to Dietary Choline Chloride in Pacific White Shrimp .

Animals (Basel) 2020 Nov 30;10(12). Epub 2020 Nov 30.

Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou 570228, China.

To determine the response of Pacific white shrimp to different levels of dietary choline, juvenile white shrimp (1.75 ± 0.09 g) were fed six semi-purified diets supplemented with 0 (control), 2000, 4000, 6000, 8000, and 12,000 mg/kg choline chloride for eight weeks. Growth performance, whole-body composition, serum characteristics and hepatopancreatic antioxidant indexes were evaluated. Meanwhile, serum metabolome and hepatopancreas transcriptome were performed to examine the overall difference in metabolite and gene expression. The weight gain, survival, specific growth rate, condition factor and hepatosomatic index were not affected by dietary choline levels. The shrimp fed 6000 mg/kg dietary choline chloride gained the maximal whole-body crude protein, which was significantly higher than that of shrimp fed with 12,000 mg/kg dietary choline. Serum total cholesterol of shrimp fed 6000 mg/kg dietary choline was higher than that in shrimp fed 4000 mg/kg choline. Dietary choline significantly decreased malondialdehyde content, superoxide dismutase, and glutathione peroxidase activities in shrimp hepatopancreas. Compared with the shrimp fed 6000 mg/kg dietary choline chloride, the glycerophospholipid metabolism pathway was significantly enriched in the shrimp fed 0 mg/kg dietary choline chloride, and the choline content and bile salt-activated lipase-like expression were upregulated. The expression of trypsin-1-like in protein digestion and absorption pathway was significantly downregulated in the shrimp fed 12,000 mg/kg dietary choline chloride. Apolipoprotein D might be a potential biomarker in shrimp, and dietary choline played an important role in lipid metabolism, especially in the reduction of oxidative damage in . Based on the results of weight gain and degree of oxidative damage, 1082 mg/kg dietary choline could meet the growth requirement of , but 2822 mg/kg dietary choline was needed to reduce peroxidation damage.
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http://dx.doi.org/10.3390/ani10122246DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760581PMC
November 2020

Recovery from Hypersaline-Stress-Induced Immunity Damage and Intestinal-Microbiota Changes through Dietary β-glucan Supplementation in Nile tilapia ().

Animals (Basel) 2020 Nov 30;10(12). Epub 2020 Nov 30.

Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou 570228, China.

Long-term exposure to hyperosmotic environments can induce severe immune damage and increase risk in tilapia breeding. As an effective immunoregulator, β-glucan has attracted extensive attention in nutritional research and given rise to high expectations of improving health status and alleviating organismal damage in tilapia, in brackish water. In this study, an 8-week cultivation experiment was conducted on tilapia fed a basal diet or diets with β-glucan supplementation in freshwater (control) and brackish water. Growth performance, hematological aspects, immune cytokine expression, and the intestinal microbiota of tilapia were analyzed. The results indicated that supplementation with β-glucan significantly reduced the enlarged spleen of tilapia resulting from hypersaline stress. Tilapia fed β-glucan showed significantly-greater decreases in the red blood cell count, hematocrit, red cell distribution width, platelet count, and plateletcrit than those fed the basal diet. β-glucan significantly decreased the high expression of immune-related genes in the spleen induced by hyperosmotic stress. In the intestine, the high migration inhibitory factor-2 () and gene expression induced by hypersaline stress was significantly reduced. β-glucan supplementation also significantly increased the abundance of beneficial microbiota such as , and . Therefore, dietary β-glucan supplementation can significantly reduce spleen enlargement and improve immune function in tilapia in brackish water. β-glucan intake can also optimize the intestinal microbiota of tilapia in brackish water and improve fish health.
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http://dx.doi.org/10.3390/ani10122243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761230PMC
November 2020

Antitumor Activity and Underlying Mechanism of Phomoxanthone B in MCF7 Cells.

Anticancer Agents Med Chem 2020 Nov 24. Epub 2020 Nov 24.

College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, Hebei 071002,. China.

Background: Xanthones are a class of heterocyclic natural products, which are promising sources of anticancer leads. Phomoxanthone B(PXB)and Phomoxanthone A(PXA)are xanthone dimers. PXA is well studied as an anti-cancer agent, but PXB is not. In our study, PXB was isolated from the endophytic fungus Phomopsis sp. By254.

Objective: The purpose of this study was to identify the underlying anti-tumor mechanisms of PXB in breast cancer MCF7 cell line.

Methods: Apoptosis, cell cycle, proliferation, invasion and migration assays were used to assess the antitumor activity of PXB. RNA sequencing was used to analyze the effect of PXB treatment on gene expression in MCF7 cells.

Results: PXB showed cytotoxicity toward a variety of tumor cells, especially MCF7 cells. PXB inhibited the migration and invasion, arrested cell cycle at G2/M phase and induced apoptosis associated with caspase-3 activation in MCF7 cells. The detailed transcriptome analysis revealed that PXB affected several pathways related to tumorigenesis, metabolisms-, and oxidative phosphorylation in MCF7 cells. KEGG transcriptome analysis revealed that PXB upregulated pro-survival signal pathways such as MAPK, PI3K-AKT and STAT3 pathways. We found that PXB also significantly upregulated the expression of IL24, DDIT3 and XAF1, which may contribute to PXB-induced apoptosis. We further found that PXB may downregulate oxidative phosphorylation by decreasing the expression of electron transport chain genes, especially MT-ND1, which is a potential unfavorable prognostic marker for ER-positive breast cancer.

Conclusion: PXB exerts strong cytotoxicity against human tumor cells and has a potential for ER-positive breast cancer treatment.
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http://dx.doi.org/10.2174/1871520620999201124215511DOI Listing
November 2020

Association between SIRT6 Methylation and Human Longevity in a Chinese Population.

Public Health Genomics 2020 25;23(5-6):190-199. Epub 2020 Nov 25.

Department of Occupational and Environmental Health, School of Public Health, Guangxi Medical University, Nanning, China.

Background: Sirtuin 6 gene (SIRT6) is a longevity gene that is involved in a variety of metabolic pathways, but the relationship between SIRT6 methylation and longevity has not been clarified.

Methods: We conducted a case-control study on 129 residents with a family history of longevity (1 of parents, themselves, or siblings aged ≥90 years) and 86 individuals without a family history of exceptional longevity to identify the association. DNA pyrosequencing was performed to analyze the methylation status of SIRT6 promoter CpG sites. qRT-PCR and ELISA were used to estimate the SIRT6 messenger RNA (mRNA) levels and protein content. Six CpG sites (P1-P6) were identified as methylation variable positions in the SIRT6 promoter region.

Results: At the P2 and P5 CpG sites, the methylation rates of the longevity group were lower than those of the control group (p < 0.001 and p = 0.009), which might be independent determinants of longevity. The mRNA and protein levels of SIRT6 decreased in the control group (p < 0.0001 and p = 0.038). The mRNA level negatively correlated with the methylation rates at the P2 (rs = -0.173, p = 0.011) and P5 sites (rs = -0.207, p = 0.002). Furthermore, the protein content positively correlated with the methylation rate at the P5 site (rs = 0.136, p = 0.046) but showed no significant correlation with the methylation rate at the P2 site.

Conclusion: The low level of SIRT6 methylation may be a potential protective factor of Chinese longevity.
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http://dx.doi.org/10.1159/000508832DOI Listing
May 2021

Quantitative DCE-MRI: an efficient diagnostic technique for evaluating early micro-environment permeability changes in ankylosing spondylitis.

BMC Musculoskelet Disord 2020 Nov 24;21(1):774. Epub 2020 Nov 24.

Department of Radiology, The Second Affiliated Hospital of Shandong First Medical University, Tai'an, 271000, Shandong, China.

Background: In the management of early inflammatory joint of ankylosing spondylitis (AS), there is a need for reliable noninvasive quantitative monitoring biomarker to closely assess status of synovitis progression. Cognizant to this,studies geared on improving techniques for quantitative evaluation of micro-environment permeability of the joint space are necessary. Such improved techniques may provide tissue perfusion as important biological parameters and can further help in understanding the origin of early changes associated with AS. The purpose of this study was to prospectively evaluate the diagnostic performance and determine longitudinal relationships of early micro-environment active in the joint space of the sacroiliac joint (SIJ) with a rat model by using quantitative dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI).

Methods: Thirty wistar male rats were randomly assigned to the model (n = 15) or control (n = 15) group. All rats underwent DCE-MRI of SIJ region at fixed time points (12, 17 and 22 weeks),between September 2018 and October 2019. Differences in permeability parameters between the two groups at the same time point were compared by using an independent samples t test. Spearman correlations of DCE-MRI parameters with different time points in model group were analyzed. All statistical analyses were performed with software.

Results: At 12 weeks,the K,K and V values in the model group were slightly lower than those in control group,but all the differences were not statistically significant (p > 0.05). Compared with control group,the transfer constant (K) values increased significantly at 17 weeks and 22 weeks in model group,while the rate constant (K) and volume of extravascular extracellular space (V) significantly increased only at 22 weeks(p < 0.05). The K,K and V were positively correlated with increasing time points (r = 0.946, P<0.01 for K; r = 0.945, P<0.01 for K; and r = 0.832, P<0.01 for V).

Conclusion: Quantitative DCE-MRI parameters are valuable for evaluating the early longitudinal relationship of micro-environment permeability changes in the joint space of SIJ.
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http://dx.doi.org/10.1186/s12891-020-03805-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7685584PMC
November 2020

YTHDF1 Facilitates the Progression of Hepatocellular Carcinoma by Promoting FZD5 mRNA Translation in an m6A-Dependent Manner.

Mol Ther Nucleic Acids 2020 Dec 4;22:750-765. Epub 2020 Oct 4.

School of Medicine, Southeast University, Nanjing 210096, Jiangsu, China.

Hepatocellular carcinoma (HCC), one of the most aggressive malignancies, ranks as the fourth leading cause of cancer-related deaths worldwide. Emerging evidence indicates that RNA N6-methyladenosine (m6A) plays a critical role in tumor progression. However, the biological function of YTHDF1 in HCC remains unclear. Here, we found that YTHDF1 expression was strikingly elevated in HCC tissues and cell lines and significantly associated with prognosis of HCC patients. Moreover, YTHDF1 expression was transcriptionally regulated by USF1 and c-MYC in HCC. Functional studies showed that YTHDF1 can promote HCC cell proliferation and metastasis both and . Multi-omics analysis revealed that YTHDF1 can accelerate the translational output of FZD5 mRNA in an m6A-dependent manner and function as an oncogene through the WNT/β-catenin pathway. Taken together, our study revealed an essential role of YTHDF1 in the progression of HCC cells, which indicated that targeting YTHDF1 may be a potential therapeutic strategy in HCC.
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http://dx.doi.org/10.1016/j.omtn.2020.09.036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595883PMC
December 2020

Modified Linggui Zhugan Decoction () Ameliorates Glycolipid Metabolism and Inflammation via PI3K-Akt/mTOR-S6K1/AMPK-PGC-1 α Signaling Pathways in Obese Type 2 Diabetic Rats.

Chin J Integr Med 2020 Nov 19. Epub 2020 Nov 19.

Department of VIP Ward, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China.

Objective: To investigate the protective effects of modified Linggui Zhugan Decoction (, MLZD), a traditional Chinese medicine formula, on obese type 2 diabetes mellitus (T2DM) rats.

Methods: Fifty Sprague-Dawley rats were randomly divided into 5 groups by a random number table, including normal, obese T2DM (ob-T2DM), MLZD low-dose [MLDZ-L, 4.625 g/(kg·d)], MLZD middle-dose [MLD-M, 9.25 g/(kg·d) ] and MLZD high-dose [MLD-H, 18.5 g/(kg·d)] groups, 10 rats in each group. After 4-week intervention, blood samples and liver, pancreas, muscle tissues were collected to assess the insulin resistance (IR), blood lipid, adipokines and inflammation cytokines. The alteration of phosphatidylinositol 3 kinase (PI3K)-protein kinase B (PKB or Akt)/the mammalian target of rapamycin (mTOR)-ribosome protein subunit 6 kinase 1 (S6K1 )/AMP-activated protein kinase (AMPK)-peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1 α) pathways were also studied.

Results: MLZD dose-dependently reduced fasting blood glucose, fasting insulin, homeostasis model of assessment for IR index and increased insulin sensitive index compared with ob-T2DM rats (P<0.05). Similarly, total cholesterol, triglyceride, low-density lipoprotein cholesterol and free fatty acids were also decreased compared with ob-T2DM rats after 4-week treatment (P<0.05 or P<0.01). Improvements in adipokines and inflammatory cytokines were observed with a raised level of adiponectin and a reduced level of leptin, resistin, tumor necrosis factor-α and interleukin-6 (P<0.05 or P<0.01). MLZD regulated the PI3K-Akt/mTOR-S6K1/AMPK-PGC-1 α pathways and restored the tissue structure of liver and pancreas (P<0.05 or P<0.01).

Conclusions: MLZD ameliorated glycolipid metabolism and inflammation, which may be attributed to the regulation of PI3K-Akt/mTOR-S6K1/AMPK-PGC-1 α pathways.
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http://dx.doi.org/10.1007/s11655-020-3285-2DOI Listing
November 2020

Genome Resource of L9-754, an Endophyte Isolated From Leaf Tissues of .

Plant Dis 2021 Jan 17;105(1):205-206. Epub 2020 Nov 17.

Key Laboratory of Southwest Rice Biology and Genetic Breeding, Ministry of Agriculture/Luzhou Branch of National Rice Improvement Center, Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Deyang 618000, P.R. China.

strain L9-754 (DSM 27347) is a gram-negative, chemoheterotrophic, and rod-shaped endophyte isolated from the stem tissues of L. This strain has putative in vitro antagonistic ability against the plant pathogenic fungus . A draft genome of L9-754T was obtained using the PacBio SMRT cell platform. By analyzing the genome of strain L9-754T, a gene cluster (GQR91_18700 - GQR91_18715) related to an antioxidant enzyme was identified in the obtained draft genome. The information obtained from the draft genome is expected to reveal the putative properties helpful in biocontrol applications.
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http://dx.doi.org/10.1094/PDIS-05-20-1052-ADOI Listing
January 2021

Analysis of METTL3 and METTL14 in hepatocellular carcinoma.

Aging (Albany NY) 2020 11 6;12(21):21638-21659. Epub 2020 Nov 6.

School of Medicine, Southeast University, Nanjing 210096, Jiangsu, China.

N6-methyladenosine (m6A) RNA methylation is the most prevalent modification of messenger RNAs (mRNAs) and catalyzed by a multicomponent methyltransferase complex (MTC), among which methyltransferase-like 3 (METTL3) and METTL14 are two core molecules. However, METTL3 and METTL14 play opposite regulatory roles in hepatocellular carcinoma (HCC). Based on The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) database, we conducted a multi-omics analysis of METTL3 and METTL14 in HCC, including RNA-sequencing, m6ARIP-sequencing, and ribosome-sequencing profiles. We found that the expression and prognostic value of METTL3 and METTL14 are opposite in HCC. Besides, after METTL3 and METTL14 knockdown, most of the dysregulated mRNAs, signaling pathways and biological processes are distinct in HCC, which partly explains the contrary regulatory role of METTL3 and METTL14. Intriguingly, these mRNAs whose stability or translation efficiency are influenced by METTL3 or METTL14 in an m6A dependent manner, jointly regulate multiple signaling pathways and biological processes, which supports the cooperative role of METTL3 and METTL14 in catalyzing m6A modification. In conclusion, our study further clarified the contradictory role of METTL3 and METTL14 in HCC.
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http://dx.doi.org/10.18632/aging.103959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695415PMC
November 2020