Publications by authors named "Jian Lv"

240 Publications

Evaluation of Feature Selection Methods for Mammographic Breast Cancer Diagnosis in a Unified Framework.

Biomed Res Int 2021 4;2021:6079163. Epub 2021 Oct 4.

Department of Radiology, Tianjin Central Hospital of Obstetrics and Gynecology, Tianjin 300100, China.

Over recent years, feature selection (FS) has gained more attention in intelligent diagnosis. This study is aimed at evaluating FS methods in a unified framework for mammographic breast cancer diagnosis. After FS methods generated rank lists according to feature importance, the framework added features incrementally as the input of random forest which performed as the classifier for breast lesion classification. In this study, 10 FS methods were evaluated and the digital database for screening mammography (1104 benign and 980 malignant lesions) was analyzed. The classification performance was quantified with the area under the curve (AUC), and accuracy, sensitivity, and specificity were also considered. Experimental results suggested that both infinite latent FS method (AUC, 0.866 ± 0.028) and RELIEFF (AUC, 0.855 ± 0.020) achieved good prediction (AUC ≥ 0.85) when 6 features were used, followed by correlation-based FS method (AUC, 0.867 ± 0.023) using 7 features and WILCOXON (AUC, 0.887 ± 0.019) using 8 features. The reliability of the diagnosis models was also verified, indicating that correlation-based FS method was generally superior over other methods. Identification of discriminative features among high-throughput ones remains an unavoidable challenge in intelligent diagnosis, and extra efforts should be made toward accurate and efficient feature selection.
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http://dx.doi.org/10.1155/2021/6079163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505067PMC
October 2021

Identification of Pulpitis-Related Potential Biomarkers Using Bioinformatics Approach.

Comput Math Methods Med 2021 29;2021:1808361. Epub 2021 Sep 29.

Department of Preventive Dentistry, Qingdao Stomatological Hospital Affiliated to Qingdao University, 266001 Qingdao, Shandong Province, China.

Inflammatory reaction of pulp tissue plays a role in the pathogen elimination and tissue repair. The evaluation of severity of pulpitis can serve an instructive function in therapeutic scheme. However, there are many limitations in the traditional evaluation methods for the severity of pulpitis. Based on the Gene Expression Omnibus (GEO) database, our study discovered 843 differentially expressed genes (DEGs) related to pulpitis. Afterwards, we constructed a protein-protein interaction (PPI) network of DEGs and used MCODE plugin to determine the key functional subset. Meanwhile, genes in the key functional subset were subjected to GO and KEGG enrichment analyses. The result showed that genes were mainly enriched in inflammatory reaction-related functions. Next, we screened out intersections of PPI network nodes and pulpitis-related genes. Then, 20 genes were obtained as seed genes. In the PPI network, 50 genes that had the highest correlation with seed genes were screened out using random walk with restart (RWR). Furthermore, 4 pulpitis-related hub genes were obtained from the intersection of the top 50 genes and genes in the key functional subset. Finally, GeneMANIA was utilized to predict genes coexpressed with hub genes, and expression levels of the 4 hub genes in normal and pulpitis groups were analyzed based on GEO data. The result demonstrated that the 4 hub genes were mainly coexpressed with chemokine-related genes and were remarkably upregulated in the pulpitis group. In short, we eventually determined 4 potential biomarkers of pulpitis.
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http://dx.doi.org/10.1155/2021/1808361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495466PMC
September 2021

Nanopipette-Based Nanosensor for Label-Free Electrochemical Monitoring of Cell Membrane Rupture under HO Treatment.

Anal Chem 2021 Oct 8. Epub 2021 Oct 8.

Key Laboratory for Advanced Materials & School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.

HO is an essential signaling molecule in living cells that can cause direct damage to lipids, proteins, and DNA, resulting in cell membrane rupture. However, current studies mostly focus on probe-based sensing of intracellular HO, and these methods usually require sophisticated probe synthesis and instruments. In particular, local HO treatment induces cell membrane rupture, but the level of cell membrane destruction is unknown because the mechanical properties of the cell membrane are difficult to accurately determine. Therefore, highly sensitive and label-free methods are required to measure and reflect mechanical changes in the cell membrane. Here, using an ultrasmall quartz nanopipette with a tip diameter less than 90 nm as a nanosensor, label-free and noninvasive electrochemical single-cell measurement is achieved for real-time monitoring of cell membrane rupture under HO treatment. By spatially controlling the nanopipette tip to precisely approach a specific location on the membrane of a single living cell, stable cyclic membrane oscillations are observed under a constant direct current voltage. Specifically, upon nanopipette advancement, the mechanical status of the cell membrane can be sensibly displayed by continuous current versus time traces. The electrical signals are collected and processed, ultimately revealing the mechanical properties of the cell membrane and the degree of cell apoptosis. This nanopipette-based nanosensor paves the way for developing a facile, label-free, and noninvasive strategy to assay the mechanical properties of the cell membrane during external stimulation at the single-cell level.
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http://dx.doi.org/10.1021/acs.analchem.1c03313DOI Listing
October 2021

Artificial intelligence manages congenital cataract with individualized prediction and telehealth computing.

NPJ Digit Med 2020 Aug 28;3(1):112. Epub 2020 Aug 28.

Department of Ophthalmology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

A challenge of chronic diseases that remains to be solved is how to liberate patients and medical resources from the burdens of long-term monitoring and periodic visits. Precise management based on artificial intelligence (AI) holds great promise; however, a clinical application that fully integrates prediction and telehealth computing has not been achieved, and further efforts are required to validate its real-world benefits. Taking congenital cataract as a representative, we used Bayesian and deep-learning algorithms to create CC-Guardian, an AI agent that incorporates individualized prediction and scheduling, and intelligent telehealth follow-up computing. Our agent exhibits high sensitivity and specificity in both internal and multi-resource validation. We integrate our agent with a web-based smartphone app and prototype a prediction-telehealth cloud platform to support our intelligent follow-up system. We then conduct a retrospective self-controlled test validating that our system not only accurately detects and addresses complications at earlier stages, but also reduces the socioeconomic burdens compared to conventional methods. This study represents a pioneering step in applying AI to achieve real medical benefits and demonstrates a novel strategy for the effective management of chronic diseases.
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http://dx.doi.org/10.1038/s41746-020-00319-xDOI Listing
August 2020

Carbon dots induced in-situ formation of porous europium micro-networks with enhanced photocatalysis.

J Colloid Interface Sci 2021 Aug 12;606(Pt 1):600-606. Epub 2021 Aug 12.

Key Laboratory for Advanced Materials, Feringa Nobel Prize Scientist Joint Research Center, Frontiers Science Center for Materiobiology & Dynamic Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address:

Excellent photocatalysts are highly desirable for clean energy and pollutant treatment. Carbon dots (CDs)-based composite photocatalysts have been widely studied and utilized in catalytic fields. However, the preparation of the photocatalysts with high catalytic activity still faces a great challenge. Herein, white-light-driven CDs-based porous europium micro-networks ([email protected]) composite photocatalysts are prepared by a facile in-situ growth strategy. CDs can affect the morphology and produce a large number of porous structures of [email protected] Importantly, the introduction of CDs not only increases the light absorption, but also promotes the separation of photogenerated charge carriers, and thus improve photocatalytic performance of [email protected] composites. [email protected] show the highest photocurrent density, which can be used for the highly-efficient photodegradation of rhodamine 6G dyes with almost 95% degradation rate under low power white light (20 W) without any radical generating agents such as HO. Therefore, this new and efficient [email protected] photocatalyst will have a great application prospect in water pollution treatment.
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http://dx.doi.org/10.1016/j.jcis.2021.08.057DOI Listing
August 2021

The flavonoid Astragalin shows anti-tumor activity and inhibits PI3K/AKT signaling in gastric cancer.

Chem Biol Drug Des 2021 Aug 15. Epub 2021 Aug 15.

Department of Radiation Oncology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong Province, China.

Gastric cancer is a common malignant cancer, which is one of the most affected cancers by PI3K/AKT signaling. Here, we investigated the anti-tumor role of Astragalin, a natural flavonoid compound, in gastric cancer and explored the underlying molecular mechanism. Three well-established gastric cancer cell lines and xenograft mouse model were used to examine the anti-tumor effect of Astragalin by using CCK-8, transwell assays, and Western blot. Tumor burden of xenograft mice with Astragalin administration was monitored and determined during and at end of the experiments. Astragalin could effectively inhibit cell viability of gastric cancer cells and possessed good anti-tumor activity in xenograft mice. In addition, astragalin induced the expression of apoptotic signaling proteins, suppressed the migration and invasion cancer cells, and inhibited the PI3K/AKT signaling pathway significantly. In contrast, epidermal growth factor stimulation was able to block the anti-tumor activity of Astragalin. In conclusion, astragalin exerts its anticancer activities through inhibiting PI3K/AKT signaling, which highlights its potential for the treatment of gastric cancer.
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http://dx.doi.org/10.1111/cbdd.13933DOI Listing
August 2021

The direct targets of CBFs: in cold stress response and beyond.

J Integr Plant Biol 2021 Aug 11. Epub 2021 Aug 11.

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.

Cold acclimation in Arabidopsis thaliana triggers a significant transcriptional reprogramming altering the expression patterns of thousands of cold-responsive (COR) genes. Essential to this process is the C-repeat binding factor (CBF)-dependent pathway, involving the activity of AP2/ERF (APETALA2/ethylene-responsive factor)-type CBF transcription factors required for plant cold acclimation. In this study, we performed chromatin immunoprecipitation assays followed by deep sequencing (ChIP-seq) to determine the genome-wide binding sites of the CBF transcription factors. Cold-induced CBF proteins specifically bind to the conserved C-repeat (CRT)/dehydration-responsive elements (CRT/DRE; G/ACCGAC) of their target genes. A Gene Ontology enrichment analysis showed that 1,012 genes are targeted by all three CBFs. Combined with a transcriptional analysis of the cbf1,2,3 triple mutant, we define 146 CBF regulons as direct CBF targets. In addition, the CBF-target genes are significantly enriched in functions associated with hormone, light, and circadian rhythm signaling, suggesting that the CBFs act as key integrators of endogenous and external environmental cues. Our findings not only define the genome-wide binding patterns of the CBFs during the early cold response, but also provide insights into the role of the CBFs in regulating multiple biological processes of plants. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/jipb.13161DOI Listing
August 2021

Physiological Responses of Cucumber Seedlings to Different Supplemental Light Duration of Red and Blue LED.

Front Plant Sci 2021 12;12:709313. Epub 2021 Jul 12.

College of Horticulture, Gansu Agricultural University, Lanzhou, China.

Normal development of plants is inhibited by inadequate light in winter in greenhouses in Northwest China. Growth lamps, using light-emitting diodes (LEDs) with red blue light (7R2B), were used to supplement daylight for 1, 2, and 3 h. Seedling growth, photosynthesis, and photosynthetic product; the Calvin cycle key and sugar metabolism-related enzymes and their encoding genes; and the light signal sensing regulation of key gene expression were studied in greenhouse cucumbers under three treatments to determine the best supplemental light durations to enhance cucumber cultivation in greenhouses in winter. Treatment with LED red and blue light for 3 h significantly promoted the growth and development of cucumbers, root growth, and dry matter accumulation. It improved the photosynthetic rate, photosynthetic pigment content, and light energy utilization efficiency in cucumbers. Supplementation with red and blue LED light for 3 h upregulated the expression levels of key genes encoding the Calvin cycle and enzymes related to sugar metabolism in cucumber leaves, which promoted the synthesis and accumulation of photosynthates. The expression levels of phytochrome B, cryptochrome 1, and hypocotyl 5 in the cucumber leaves were also significantly upregulated after 3 h of light supplementation. Combined LED red and blue light for 3 h should be used to supplement natural light to enhance the cucumber cultivation in greenhouses in winter.
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http://dx.doi.org/10.3389/fpls.2021.709313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311605PMC
July 2021

Printable elastomeric electrodes with sweat-enhanced conductivity for wearables.

Sci Adv 2021 Jul 14;7(29). Epub 2021 Jul 14.

School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.

We rationally synthesized the thermoplastic and hydrophilic poly(urethane-acrylate) (HPUA) binder for a type of printable and stretchable Ag flakes-HPUA (Ag-HPUA) electrodes in which the conductivity can be enhanced by human sweat. In the presence of human sweat, the synergistic effect of Cl and lactic acid enables the partial removal of insulating surfactant on silver flakes and facilitates sintering of the exposed silver flakes, thus the resistance of Ag-HPUA electrodes can be notably reduced in both relaxed and stretched state. The on-body data show that the resistance of one electrode has been decreased from 3.02 to 0.62 ohm during the subject's 27-min sweating activity. A stretchable textile sweat-activated battery using Ag-HPUA electrodes as current collectors and human sweat as the electrolyte was constructed for wearable electronics. The enhanced conductivity of the wearable wiring electrode from the reaction with sweat would provide meritorious insight into the design of wearable devices.
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http://dx.doi.org/10.1126/sciadv.abg8433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8279513PMC
July 2021

Binary-Acid Catalysis with Sc(OTf)/TfOH in the Alkenylation of Arenes with Alkynes.

Org Lett 2021 Aug 8;23(15):5998-6003. Epub 2021 Jul 8.

Key Laboratory of Optic-electric Sensing and Analytic Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science & Technology, Qingdao 266042, China.

Herein we report binary acid Sc(OTf)/TfOH-catalyzed alkenylation of arenes with alkynes. In this system, the high-energy vinyl carbocations with activated and weakly coordinating trifluoromethanesulfonate anions by Lewis acid Sc(III) can undergo facile Friedel-Crafts reactions with arenes to give the desired adducts in up to 90% yield and with high -selectivity.
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http://dx.doi.org/10.1021/acs.orglett.1c02065DOI Listing
August 2021

A deep transfer learning framework for the automated assessment of corneal inflammation on in vivo confocal microscopy images.

PLoS One 2021 3;16(6):e0252653. Epub 2021 Jun 3.

Guangxi Health Commission Key Laboratory of Ophthalmology and Related Systemic Diseases Artificial Intelligence Screening Technology, Ophthalmology Department, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China.

Purpose: Infiltration of activated dendritic cells and inflammatory cells in cornea represents an important marker for defining corneal inflammation. Deep transfer learning has presented a promising potential and is gaining more importance in computer assisted diagnosis. This study aimed to develop deep transfer learning models for automatic detection of activated dendritic cells and inflammatory cells using in vivo confocal microscopy images.

Methods: A total of 3453 images was used to train the models. External validation was performed on an independent test set of 558 images. A ground-truth label was assigned to each image by a panel of cornea specialists. We constructed a deep transfer learning network that consisted of a pre-trained network and an adaptation layer. In this work, five pre-trained networks were considered, namely VGG-16, ResNet-101, Inception V3, Xception, and Inception-ResNet V2. The performance of each transfer network was evaluated by calculating the area under the curve (AUC) of receiver operating characteristic, accuracy, sensitivity, specificity, and G mean.

Results: The best performance was achieved by Inception-ResNet V2 transfer model. In the validation set, the best transfer system achieved an AUC of 0.9646 (P<0.001) in identifying activated dendritic cells (accuracy, 0.9319; sensitivity, 0.8171; specificity, 0.9517; and G mean, 0.8872), and 0.9901 (P<0.001) in identifying inflammatory cells (accuracy, 0.9767; sensitivity, 0.9174; specificity, 0.9931; and G mean, 0.9545).

Conclusions: The deep transfer learning models provide a completely automated analysis of corneal inflammatory cellular components with high accuracy. The implementation of such models would greatly benefit the management of corneal diseases and reduce workloads for ophthalmologists.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252653PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174724PMC
June 2021

The Roles of Cruciferae Glucosinolates in Disease and Pest Resistance.

Plants (Basel) 2021 May 30;10(6). Epub 2021 May 30.

Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.

With the expansion of the area under Cruciferae vegetable cultivation, and an increase in the incidence of natural threats such as pests and diseases globally, Cruciferae vegetable losses caused by pathogens, insects, and pests are on the rise. As one of the key metabolites produced by Cruciferae vegetables, glucosinolate (GLS) is not only an indicator of their quality but also controls infestation by numerous fungi, bacteria, aphids, and worms. Today, the safe and pollution-free production of vegetables is advocated globally, and environmentally friendly pest and disease control strategies, such as biological control, to minimize the adverse impacts of pathogen and insect pest stress on Cruciferae vegetables, have attracted the attention of researchers. This review explores the mechanisms via which GLS acts as a defensive substance, participates in responses to biotic stress, and enhances plant tolerance to the various stress factors. According to the current research status, future research directions are also proposed.
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http://dx.doi.org/10.3390/plants10061097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229868PMC
May 2021

Effects of Vertical Lifting Distance on Upper-Body Muscle Fatigue.

Int J Environ Res Public Health 2021 05 20;18(10). Epub 2021 May 20.

Key Laboratory of Advanced Manufacturing Technology of the Ministry of Education, Guizhou University, Guiyang 550025, China.

Manual material handling (MMH) is commonly demanded in the manufacturing industry. Occupational muscle fatigue of the arm, shoulder, and back, which arise from MMH tasks, can cause work absences and low efficiency. The available literature presents the lack of the fatigue comparison between targeted muscles, on the same part or on different parts. The main aim of the present study was to evaluate and compare the fatigue of upper-body muscles during repetitive bending tasks, an experiment involving 12 male subjects has been conducted to simulate material handling during furniture board drilling. The vertical lifting distance was chosen to be the single independent variable, and the three levels were 0, 250, and 500 mm. Surface electromyography (sEMG) was used to measure the muscle fatigue of the biceps brachii, upper trapezius, and multifidus, while the sEMG parameters, including the normalized electromyographic amplitude (Normalized EA) and mean power frequency (MPF), of the target muscles were analyzed. The experimental results reveal that during the manual handling tasks, the biceps brachii was the most relaxed muscle, contributing the least muscle tension, while the multifidus was the most easily fatigued muscle. Furthermore, the EMG MPF fatigue threshold (MPFFT) of multifidus muscle tension was tested to estimate its maximum workload in the long-term muscle contraction. In conclusion, bending angle should be maintained to a small range or bending should even be avoided during material-handling tasks.
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http://dx.doi.org/10.3390/ijerph18105468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160884PMC
May 2021

Vitamin D receptor gene polymorphisms and its interactions with environmental factors on renal cell carcinoma risk.

Genes Environ 2021 May 18;43(1):19. Epub 2021 May 18.

Department of Urology, Linyi cancer hospital, No.6 Lingyuan East Street, Lanshan District, Shandong Province, Linyi CIty, China.

Aims: We designed a case-control study to investigate the effect of vitamin D receptor gene (VDR) gene single nucleotide polymorphisms (SNPs) and possible gene- environment interaction on the susceptibility of renal cell carcinoma (RCC).

Methods: Generalized multifactor dimensionality reduction (GMDR) was used to find out the interaction combinations between SNPs and environmental factors, including gene- gene synergy and gene environment synergy effect. Logistic regression was used to analyze the correlation between the four SNPs in VDR gene and RCC, and the significant interaction combinations found by GMDR model were analyzed by hierarchical analysis.

Results: The genotype distribution of the control group was in accordance with Hardy- Weinberg equilibrium. Logistic regression analysis showed that the risk of RCC in VDR-rs7975232 A allele carriers was significantly higher than that of CC genotype carriers (CA + AA vs. CC), adjusted OR (95 % CI) = 1.75 (1.26-2.28). We used GMDR model to screen the best synergistic model between the four SNPs of VDR gene and smoking and drinking. We found a significant two locus model (P = 0.0010) involving rs7975232 and smoking. The cross- validation consistency of the two- locus model was 10/ 10, and the accuracy was 60.72 %. Compared with non-smokers with rs7975232 -CA or AA genotype, smokers with rs7975232 -CC genotype had the highest risk of RCC, or (95 % CI) = 2.23 (1.42-3.09), after adjustment for covariates.

Conclusions: We found that the A allele of rs7975232 within VDR gene, interaction between rs7975232 and smoking were all associated with increased RCC risk.
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http://dx.doi.org/10.1186/s41021-021-00185-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130296PMC
May 2021

Bigu-Style Fasting Affects Metabolic Health by Modulating Taurine, Glucose, and Cholesterol Homeostasis in Healthy Young Adults.

J Nutr 2021 Aug;151(8):2175-2187

Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, China.

Background: Dynamic orchestration of metabolic pathways during continuous fasting remains unclear.

Objective: We investigated the physiological effects of Bigu-style fasting and underlying metabolic reprogramming in healthy adults.

Methods: We conducted a 5-d Bigu trial in 43 healthy subjects [age 23.2 ± 2.4 y; BMI (in kg/m2) 22.52 ± 1.79]. Physiological indicators and body composition were monitored daily during fasting day 1 (F1D) to F5D and after 10-d refeeding postfasting (R10D) and R30D. Blood samples were collected in the morning. Risk factors associated with inflammation, aging, cardiovascular diseases, malnutrition, and organ dysfunction were evaluated by biochemical measurements. Untargeted plasma metabolomics and gut microbial profiling were performed using plasma and fecal samples. Data were analyzed by repeated measures ANOVA with Greenhouse-Geisser correction. Correlation analyses for metabolite modules and taurine were analyzed by Spearman's rank and Pearson tests, respectively.

Results: Heart rate was accelerated throughout the fasting period. Risk factors associated with inflammation and cardiovascular diseases were significantly lowered during or after Bigu (P < 0.05). Body composition measurement detected an overconsumption of fat starting from F3D till 1 mo after refeeding. Metabolomics unveiled a coupling between gluconeogenesis and cholesterol biosynthesis beyond F3D. Plasma taurine significantly increased at F3D by 31%-46% followed by a reduction to basal level at F5D (P < 0.001), a pattern inversely correlated with changes in glucose and de novo synthesized cholesterol (r = -0.407 and -0.296, respectively; P < 0.001). Gut microbial profiling showed an enrichment of taurine-utilizing bacteria at F5D, which was completely recovered at R10D.

Conclusions: Our data demonstrate that 5-d Bigu is potentially beneficial to health in young adults. A starvation threshold of 3-d fasting is necessary for maintaining glucose and cholesterol homeostasis via a taurine-microbiota regulatory loop. Our findings provide novel insights into the physiological and metabolic responses of the human body to continuous Bigu-style fasting. This trial was registered at http://www.chictr.org.cn as ChiCTR1900022917.
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http://dx.doi.org/10.1093/jn/nxab123DOI Listing
August 2021

Case Report: Vertebral Artery Dissection After Intravascular Stenting of the Contralateral Unruptured Vertebral Artery Aneurysm.

Front Neurol 2021 23;12:599197. Epub 2021 Apr 23.

Department of Interventional Neuroradiology, Beijing Neurosurgical Institute, Beijing Tian Tan Hospital, Capital Medical University, Beijing, China.

Spontaneous vertebral artery dissecting aneurysm has been increasingly attributed as a major cause of focal neurological deficits due to vertebrobasilar artery ischemia or subarachnoid hemorrhage (SAH). Although the development of spontaneous vertebral artery dissecting aneurysm (VADA) is rare, VADA after treatment of contralateral vertebral artery (VA) is more less frequently observed. There are only a few reports related to VADA after treatment of the contralateral VA in the medical literature. The mechanisms responsible for dissection after treatment of unilateral VADA are still not clearly understood. In this manuscript, we report an unusual case of a patient with a VADA after placement of a pipeline embolization device (PED) stent on the contralateral VA along with a thorough review of the literature. A 42-years old male patient was referred to the hospital with sudden onset of dizziness, nausea, and vomiting. Initial digital subtraction angiography (DSA) images demonstrated a VADA in the fourth segment of the left VA without the involvement of the posterior inferior cerebellar artery (PICA). There were no significant abnormalities found in the right vertebral artery. He underwent an endovascular pipeline embolization to treat the dissecting aneurysm (DA). Surprisingly, follow-up DSA imaging 14 months after the initial treatment showed a segmental dilatation and narrowing of the right VA, which suggested a VADA on the right side that had occurred postoperatively. This was followed by a tent-assisted coil embolization therapy for occluding this VADA. This patient showed an uneventful postoperative course with no neurological abnormalities. In addition to hemodynamic stress changes, the unique clinicopathological features of dissecting aneurysms may contribute significantly to the pathogenesis of VA dissection. Given that VA in VADA patients may be vulnerable on both sides, it is important to consider the risk of dissection after initial aneurysm treatment. The bilateral vertebral artery has to be carefully observed when treating any VADA patient to prevent any complications.
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http://dx.doi.org/10.3389/fneur.2021.599197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102744PMC
April 2021

The short-term efficacy of electrical pudendal nerve stimulation versus intravesical instillation for the urethral pain syndrome: a randomized clinical trial.

World J Urol 2021 Oct 2;39(10):3993-3998. Epub 2021 May 2.

The Clinical Research Section, Shanghai Research Institute of Acupuncture and Meridian, 650 South Wanping Road, Shanghai, China.

Purpose: Urethral pain syndrome is a chronic condition characterized by disturbing feeling or server pain sensed at the urethra without specific treatment. This double-center, two-arm controlled trial aimed to explore the efficacy of electrical pudendal nerve stimulation (EPNS) versus intravesical instillation (II) of heparin and alkalinized lidocaine for urethral pain syndrome (UPS).

Methods: Eighty eligible patients took three sessions of EPNS, or 1 session of II per week, for 6 consecutive weeks. The primary end point was the change of pelvic pain and urgency/frequency symptom (PUF) score from baseline to week 6. Secondary outcome measures included changes of visual analogue scale (VAS) score and three sub-score extracted from PUF score.

Results: The enrolled participants were all included in the intention-to-treat analyses, and baseline characteristics between the two groups were well balanced. The post-treatment PUF score decreased by 10.0 (7.00, 16.50) in the EPNS group, and by 7.0 (3.00, 10.00) in the II group. At the closure of treatment, the medians of changes in symptom score, bother score, pain-related score and VAS score were 6.50 (4.25, 10.00), 4.00 (2.00, 6.00), 6.00 (5.00, 8.00),4.50 (2.25, 6.00), respectively, in the EPNS group, and 4.00 (2.00, 7.00), 3.00 (1.00, 3.00), 3.00 (2.00, 6.00), 2.00 (1.00, 4.00), respectively, in the II group. All the between-group differences were statistically significant.

Conclusion: Compared with the II, the EPNS results in superior pain control and better relief of lower urinary tract symptoms, and deserves further attention.

Trial Registration: ClinicalTrials.gov (NCT03671993).
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http://dx.doi.org/10.1007/s00345-021-03698-2DOI Listing
October 2021

Hsa_circ_0004831 downregulation is partially responsible for atorvastatinalleviated human umbilical vein endothelial cell injuries induced by ox-LDL through targeting the miR-182-5p/CXCL12 axis.

BMC Cardiovasc Disord 2021 05 1;21(1):221. Epub 2021 May 1.

The Second School of Clinical Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou, China.

Background: The dysfunction and injury of human umbilical vein endothelial cells (HUVECs) are key events of atherosclerosis (AS). Atorvastatin (ATV) has been shown to play a protective role on endothelial cells. However, the associated molecular mechanisms remain not fully illustrated.

Methods: HUVECs were treated with oxidized low-density lipoprotein (ox-LDL) to mimic the pathological conditions of endothelial cell injury in AS. Cell injuries were assessed according to cell viability, cell apoptosis, cycle progression, oxidative stress and inflammatory responses using CCK-8 assay, flow cytometry assay or commercial kits. The expression of hsa_circ_0004831, miR-182-5p, and C-X-C motif chemokine 12 (CXCL12) mRNA was examined using quantitative real-time PCR (qPCR). The expression of CXCL12 protein was quantitated by western blot. The predicted target relationship between miR-182-5p and hsa_circ_0004831 or CXCL12 was verified by pull-down assay, dual-luciferase reporter assay or RIP assay.

Results: The expression of hsa_circ_0004831 was upregulated by ox-LDL but downregulated by ATV in HUVECs. ATV promoted cell viability and cell cycle progression but inhibited apoptosis, oxidative stress and inflammation in ox-LDL-treated HUVECs, while the role of ATV was partially reversed by hsa_circ_0004831 overexpression. MiR-182-5p was targeted by hsa_circ_0004831, and hsa_circ_0004831 overexpression-restored apoptosis, oxidative stress and inflammation were blocked by miR-182-5p restoration. Further, CXCL12 was targeted by miR-182-5p, and miR-182-5p inhibition-stimulated apoptosis, oxidative stress and inflammation were lessened by CXCL12 knockdown.

Conclusion: Hsa_circ_0004831-targeted miR-182-5p/CXCL12 regulatory network is one of the pathways by which ATV protects against ox-LDL-induced endothelial injuries.
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http://dx.doi.org/10.1186/s12872-021-01998-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088699PMC
May 2021

Crtc1 Deficiency Causes Obesity Potentially via Regulating PPARγ Pathway in White Adipose.

Front Cell Dev Biol 2021 12;9:602529. Epub 2021 Apr 12.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.

Obesity is characterized by excessive fat accumulation and associated with glucose and lipid metabolism disorders. Crtc1, a transcription cofactor regulating CREB activity, has been involved in the pathogenesis of metabolic syndrome; however, the underlying mechanism remains under debate. Here we generated a Crtc1 mouse line using the CRISPR/Cas9 system. Under normal feeding conditions, Crtc1 mice exhibited an obese phenotype resultant from the abnormal expansion of the white adipocytes. The development of obesity in Crtc1 mice is independent of alterations in food intake or energy expenditure. Moreover, Crtc1 mice were more prone to insulin resistance and dyslipidemia, as evidenced by higher levels of plasma glucose, insulin and FABP4 than wildtype mice. Transcriptome analysis in liver and epididymal white adipose tissue (eWAT) showed that the fat accumulation caused by Crtc1 deletion was mainly related to lipid metabolism in adipose tissue, but not in liver. GSEA and KEGG analysis identified PPAR pathway to be of the highest impact on lipid metabolism in eWAT. This regulation was independent of a direct interaction between CRTC1 and PPARγ. Our findings demonstrate a crucial role of Crtc1 in regulating lipid metabolism in adipose during development, and provide novel insights into obesity prevention and therapeutics.
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http://dx.doi.org/10.3389/fcell.2021.602529DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8075410PMC
April 2021

Clinical application of a multi-groove silicone drain combined with unidirectional negative-pressure drainage system in single-operating-port video-assisted thoracoscopic lung cancer surgery: a comparison study.

J Int Med Res 2021 Apr;49(4):300060521999766

Department of Thoracic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, P.R. China.

Objective: To evaluate the effect of a new negative-pressure drainage system in thoracoscopic lung cancer surgery; thereby, providing a new option for postoperative drainage.

Methods: We retrospectively analyzed data for 200 patients who underwent thoracoscopic surgery between May 2018 and October 2019. According to the thoracic drainage method, the patients were divided into the thoracic tube group and the new system group. The epidemiological and clinicopathological data were compared before operation, and the clinical effect of thoracic drainage was compared after operation.

Results: There was no significant difference in epidemiological and clinicopathological data between the two groups. There was also no significant difference in drain removal time, hospital stay, and complication rates between the two groups. However, the incidences of pleural effusion and poor incision healing in the new system group were lower than in the thoracic tube group. Visual analog scale (VAS) scores in the new system group were lower than those in the thoracic tube group at each postoperative interval; therefore, the new system group required less analgesia.

Conclusion: The new system was not inferior to thoracic tubes regarding the drainage effect after thoracoscopic lung cancer surgery. Hence, the system is an alternative to traditional thoracic tubes.
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http://dx.doi.org/10.1177/0300060521999766DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108081PMC
April 2021

Melatonin alleviates imidacloprid phytotoxicity to cucumber (Cucumis sativus L.) through modulating redox homeostasis in plants and promoting its metabolism by enhancing glutathione dependent detoxification.

Ecotoxicol Environ Saf 2021 Jul 23;217:112248. Epub 2021 Apr 23.

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China. Electronic address:

Melatonin (Mel), a powerful antioxidant that has the ability to regulate physiological and biochemical processes in plants under abiotic stresses. However, its roles in pesticide detoxification is poorly understood. Herein, selecting leaf spraying insecticide imidacloprid (IMD) as the model, we demonstrated the detoxification mechanism underlying root pretreatment of Mel on IMD in cucumber. IMD treatment affected the primary light conversion efficiency of photosystem II (Fv/Fm), reduced the quantum yield, and increased hydrogen peroxide and superoxide anions contents as well as the levels of membrane lipid peroxidation, indicating that excessive IMD treatment induces oxidative stress. Nonetheless, by increasing the appropriate levels of exogenous Mel, the photosynthesis of cucumber under IMD treatment reached the control levels, effectively removing reactive oxygen species. Furthermore, the content and ratio of ascorbate (AsA) and glutathione (GSH) were decreased under IMD treatment; Mel treatment enhanced the AsA/DHA and GSH/GSSG ratios, as well as the activities of MDHAR, DHAR and GR, suggesting that Mel could alleviate oxidative stress of cucumber treated with IMD by regulating the ascorbic acid-glutathione cycle. Importantly, IMD degradation rate and glutathione S-transferase (GST) activity increased after Mel treatment. The levels of transcripts encoding antioxidant enzymes GPX and GST (GST1,2 and 3) were also increased, indicating that Mel accelerated IMD degradation. These results suggest that Mel plays an important role in the detoxification of IMD by promoting GST activity and transcription and the AsA-GSH cycle, thus providing an approach for plants to reduce IMD residue through the plant's own detoxification mechanism.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112248DOI Listing
July 2021

Highly efficient generation of bacterial leaf blight-resistant and transgene-free rice using a genome editing and multiplexed selection system.

BMC Plant Biol 2021 Apr 24;21(1):197. Epub 2021 Apr 24.

Syngenta Biotechnology (China) Co., Ltd, No.25, Life Science Park Road, Beijing, 102206, China.

Background: Rice leaf blight, which is a devastating disease worldwide, is caused by the bacterium Xanthomonas oryzae pv. oryzae (Xoo). The upregulated by transcription activator-like 1 (UPT) effector box in the promoter region of the rice Xa13 gene plays a key role in Xoo pathogenicity. Mutation of a key bacterial protein-binding site in the UPT box of Xa13 to abolish PXO99-induced Xa13 expression is a way to improve rice resistance to bacteria. Highly efficient generation and selection of transgene-free edited plants are helpful to shorten and simplify the gene editing-based breeding process. Selective elimination of transgenic pollen of T0 plants can enrich the proportion of T1 transgene-free offspring, and expression of a color marker gene in seeds makes the selection of T2 plants very convenient and efficient. In this study, a genome editing and multiplexed selection system was used to generate bacterial leaf blight-resistant and transgene-free rice plants.

Results: We introduced site-specific mutations into the UPT box using CRISPR/Cas12a technology to hamper with transcription-activator-like effector (TAL) protein binding and gene activation and generated genome-edited rice with improved bacterial blight resistance. Transgenic pollen of T0 plants was eliminated by pollen-specific expression of the α-amylase gene Zmaa1, and the proportion of transgene-free plants increased from 25 to 50% among single T-DNA insertion events in the T1 generation. Transgenic seeds were visually identified and discarded by specific aleuronic expression of DsRed, which reduced the cost by 50% and led to up to 98.64% accuracy for the selection of transgene-free edited plants.

Conclusion: We demonstrated that core nucleotide deletion in the UPT box of the Xa13 promoter conferred resistance to rice blight, and selection of transgene-free plants was boosted by introducing multiplexed selection. The combination of genome editing and transgene-free selection is an efficient strategy to accelerate functional genomic research and plant breeding.
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http://dx.doi.org/10.1186/s12870-021-02979-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066475PMC
April 2021

Sulfonylation of Aryl Halides by Visible Light/Copper Catalysis.

Org Lett 2021 May 21;23(9):3663-3668. Epub 2021 Apr 21.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, P. R. China.

An efficient visible-light-assisted, copper-catalyzed sulfonylation of aryl halides with sulfinates is reported. In our protocol, a single ligand Cu photocatalyst formed in situ was used in the photocatalytic transformation. Diverse organosulfones were obtained in moderate to good yields. This strategy demonstrates a promising approach toward the synthesis of diverse and useful organosulfones.
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http://dx.doi.org/10.1021/acs.orglett.1c01050DOI Listing
May 2021

Reciprocal regulation between the negative regulator PP2CG1 phosphatase and the positive regulator OST1 kinase confers cold response in Arabidopsis.

J Integr Plant Biol 2021 Aug 31;63(8):1568-1587. Epub 2021 May 31.

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.

Protein phosphorylation and dephosphorylation have been reported to play important roles in plant cold responses. In addition, phospho-regulatory feedback is a conserved mechanism for biological processes and stress responses in animals and plants. However, it is less well known that a regulatory feedback loop is formed by the protein kinase and the protein phosphatase in plant responses to cold stress. Here, we report that OPEN STOMATA 1 (OST1) and PROTEIN PHOSPHATASE 2C G GROUP 1 (PP2CG1) reciprocally regulate the activity during the cold stress response. The interaction of PP2CG1 and OST1 is inhibited by cold stress, which results in the release of OST1 at the cytoplasm and nucleus from suppression by PP2CG1. Interestingly, cold-activated OST1 phosphorylates PP2CG1 to suppress its phosphatase activity, thereby amplifying cold signaling in plants. Mutations of PP2CG1 and its homolog PP2CG2 enhance freezing tolerance, whereas overexpression of PP2CG1 decreases freezing tolerance. Moreover, PP2CG1 negatively regulates protein levels of C-REPEAT BINDING FACTORs (CBFs) under cold stress. Our results uncover a phosphor/dephosphor-regulatory feedback loop mediated by PP2CG1 phosphatase and OST1 protein kinase in plant cold responses.
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http://dx.doi.org/10.1111/jipb.13100DOI Listing
August 2021

Effect of slow-release fertilizer on soil fertility and growth and quality of wintering Chinese chives (Allium tuberm Rottler ex Spreng.) in greenhouses.

Sci Rep 2021 04 13;11(1):8070. Epub 2021 Apr 13.

College of Horticulture, Gansu Agricultural University, Yingmen Village, Anning District, Lanzhou, 730070, China.

To avoid the negative impact of excessive fertilization on vegetable production, a decreased fertilization experiment was conducted in a multi-layer covered plastic greenhouse in 2017 to 2018. Treatments included no fertilizer (CK), traditional fertilization (TF), slow-release fertilizers (SRF), and decreased fertilization with slow-release fertilizers (DSRF). Results showed that the SRF and DSRF increased leaf length (13% and 8.3%) and chlorophyll content (7.1% and 8.2%) of Chinese chives compared to TF. Similarly, DSRF was found to increase the accumulation of dry matter accumulation of roots (22%) and the dry matter accumulation of shoots (36%) of Chinese chives. Flavonoid, soluble sugar, and soluble protein content were enhanced by 18%, 8.5%, and 4.6%, respectively, in DSRF compared to TF. Nitrate content of the SRF and SRFR decreased significantly by 26% and 35%, respectively. In addition, there was a significant increase in soil nutrient and enzyme activity in the middle and late harvest of Chinese chives under DSRF compared to TF, and there was a high correlation between soil nutrients and the quality of Chinese chives. The available P and total P content significantly differed among the different greenhouse soil samples, and this significantly affected the quality of Chinese chives. The content of available P and total P in greenhouse soil was 125.07 g kg and 1.26 mg kg, respectively. Optimal quality was obtained. Hence, the application of DSRF promoted the growth of Chinese chives and improved soil fertility, thereby enhancing the productivity and quality of Chinese chives.
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http://dx.doi.org/10.1038/s41598-021-87593-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044230PMC
April 2021

Identification and validation of the prognostic value of cyclic GMP-AMP synthase-stimulator of interferon (cGAS-STING) related genes in gastric cancer.

Bioengineered 2021 12;12(1):1238-1250

Department of General Surgery, People's Hospital of Jingjiang, Yangzhou University Medical Academy, Jingjiang, China.

The cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway play a significant role in the production of inflammatory cytokines and type I interferons. This study aims to develop a cGAS-STING pathway-related genes (CSRs) prediction model to predict prognosis in gastric cancer (GC). In the present study, we used The Cancer Genome Atlas (TCGA), Gene Expression Omnibus databases (GEO), CIBERSORT and Tumor Immune Estimation Resource databases (TIMER). The risk model based on five hub genes (IFNB1, IFNA4, IL6, NFKB2, and TRIM25) was constructed to predict the overall survival (OS) of GC. Further univariate Cox regression (URC) and multivariate Cox regression (MCR) analyses revealed that this risk scoring model was an independent factor. The results were verified by GEO external validation set. Multiple immune pathways were assessed by Gene Set Enrichment Analysis (GSEA). TIMER analysis demonstrated that risk score strongly correlated with Macrophage, B cells and CD8 + T cells infiltration. In addition, through 'CIBERSORT' package, the higher levels of infiltration of T cell follicular assistance (P = 0.011), NK cells-activated (P = 0.034), and Dendritic cells resting (P = 0.033) exhibited in high-risk group. Kaplan-Meier (K-M) survival analysis illustrated T cells CD4 memory resting and T cells follicular helper infiltration correlated with overall survival (OS) of GC patients in TCGA and GEO databases. Altogether, the risk score model can be conveniently used to predict prognosis. The immunocyte infiltration analysis provided a novel horizon for monitoring the status of the GC immune microenvironment.:TCGA: The Cancer Genome Atlas databases; GEO: Gene Expression Omnibus databases; GC: Gastric cancer; CSRs: cGAS-STING pathway-related genes; DECSRs: Differential expressed cGAS-STING pathway-related genes; PCSRs: Prognosis related cGAS-STING pathway genes; URC: Univariate Cox regression analyses; MCR: Multivariate Cox regression analyses GSEA: Gene set enrichment analysis; TIIC: Tumor-infiltrating immune cell.
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http://dx.doi.org/10.1080/21655979.2021.1911557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291813PMC
December 2021

A self-sustainable wearable multi-modular E-textile bioenergy microgrid system.

Nat Commun 2021 03 9;12(1):1542. Epub 2021 Mar 9.

Department of Nanoengineering, Center of Wearable Sensors, University of California San Diego, La Jolla, CA, USA.

Despite the fast development of various energy harvesting and storage devices, their judicious integration into efficient, autonomous, and sustainable wearable systems has not been widely explored. Here, we introduce the concept and design principles of e-textile microgrids by demonstrating a multi-module bioenergy microgrid system. Unlike earlier hybrid wearable systems, the presented e-textile microgrid relies solely on human activity to work synergistically, harvesting biochemical and biomechanical energy using sweat-based biofuel cells and triboelectric generators, and regulating the harvested energy via supercapacitors for high-power output. Through energy budgeting, the e-textile system can efficiently power liquid crystal displays continuously or a sweat sensor-electrochromic display system in pulsed sessions, with half the booting time and triple the runtime in a 10-min exercise session. Implementing "compatible form factors, commensurate performance, and complementary functionality" design principles, the flexible, textile-based bioenergy microgrid offers attractive prospects for the design and operation of efficient, sustainable, and autonomous wearable systems.
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http://dx.doi.org/10.1038/s41467-021-21701-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943583PMC
March 2021

Alleviating damage of photosystem and oxidative stress from chilling stress with exogenous zeaxanthin in pepper (Capsicum annuum L.) seedlings.

Plant Physiol Biochem 2021 May 9;162:395-409. Epub 2021 Mar 9.

College of Horticulture, Gansu Agricultural University, Yingmen Village, Anning District, Lanzhou, 730070, PR China.

As a typical thermophilous vegetable, the growth and yield of peppers are easily limited by chilling conditions. Zeaxanthin, a crucial carotenoid, positively regulates plant abiotic stress responses. Therefore, this study investigated the regulatory mechanisms of zeaxanthin-induced chilling tolerance in peppers. The results indicated that the pretreatment with zeaxanthin effectively alleviated chilling damage in pepper leaves and increased the plant fresh weight and photosynthetic pigment content under chilling stress. Additionally, alterations in photosynthetic chlorophyll fluorescence parameters and chlorophyll fluorescence induction curves after zeaxanthin treatment highlighted the participation of zeaxanthin in improving the photosystem response to chilling stress by heightening the quenching of excess excitation energy and protection of the photosynthetic electron transport system. In chill-stressed plants, zeaxanthin treatment also enhanced antioxidant enzyme activity and transcript expression, and reduced hydrogen peroxide (HO) and superoxide anion (O) content, resulting in a decrease in biological membrane damage. Additionally, exogenous zeaxanthin upregulated the expression levels of key genes encoding β-carotene hydroxylase (CaCA1, CaCA2), zeaxanthin epoxidase (CaZEP) and violaxanthin de-epoxidase (CaVDE), and promoted the synthesis of endogenous zeaxanthin during chilling stress. Collectively, exogenous zeaxanthin pretreatment enhances plant tolerance to chilling by improving the photosystem process, increasing oxidation resistance, and inducing alterations in endogenous zeaxanthin metabolism.
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http://dx.doi.org/10.1016/j.plaphy.2021.03.010DOI Listing
May 2021

Breathable Nanogenerators for an On-Plant Self-Powered Sustainable Agriculture System.

ACS Nano 2021 03 9;15(3):5307-5315. Epub 2021 Mar 9.

School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore.

Building an intelligent interface between plants and the environment is of paramount importance for real-time monitoring of the health status of plants, especially promising for high agricultural yield. Although the advancement of various sensors allows automated monitoring, developing a sustainable power supply for these electronic devices remains a formidable challenge. Herein, a waterproof and breathable triboelectric nanogenerator (WB-TENG) is designed based on poly(vinylidene fluoride--hexafluoropropylene) (PVDF-HFP) nanofibers embedded with fluorinated carbon nanotubes (F-CNT) microspheres, which was realized by simultaneous electrospinning and electrospraying, respectively. Using carbon nanotubes (CNT) as the electrode, the WB-TENG shows micro-to-nano hierarchical porous structures and high electrostatic adhesion, exhibiting a high output power density of 330.6 μW cm, breathability, and hydrophobicity. Besides, the WB-TENG can be conformally self-attached to plant leaves without sacrificing the intrinsic physiological activities of plants, capable of harvesting typical environmental energy from wind and raindrops. Results demonstrate that the WB-TENG can serve as a sustainable power supply for a wireless plant sensor, enabling real-time monitoring of the health status of plants. This work realizes the concept of constructing a plant compatible TENG with environment adaptivity and energy scavenging ability, showing great potential in building a self-powered agriculture system.
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http://dx.doi.org/10.1021/acsnano.0c10817DOI Listing
March 2021

A Retrospective Study of Lung Transplantation in Patients With Lymphangioleiomyomatosis: Challenges and Outcomes.

Front Med (Lausanne) 2021 16;8:584826. Epub 2021 Feb 16.

Wuxi Lung Transplant Center, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, China.

Lymphangioleiomyomatosis (LAM) is a rare systemic disease that generally leads to a progressive decline in pulmonary function. Experience, especially from the Asian population, including combined drug therapy before and after lung transplantation (LT) in LAM, is still limited. This study aimed to summarize the clinical data from patients with pulmonary LAM who underwent LT at centers in China. A retrospective review of all patients with LAM undergoing LT at the two largest centers in China between 2010 and 2018 was conducted. Pre- and posttransplant data were assessed and analyzed. Overall, 25 patients with LAM underwent bilateral LT. The mean age was 35.0 ± 8.6 years at diagnosis and 36.8 ± 9.3 years at the time of transplant. Before LT, only six patients could complete pulmonary function test; the reachable mean forced expiratory volume in one second (FEV) before LT was 15.9 ± 6.9%. Twenty-one patients (84%) had a recurrent pneumothorax, four (16.0%) of which required pleurodesis. Eight patients (32%) were treated with sirolimus pretransplant for 3.9 years (1-9 years). The average intra-surgery bleeding volume was 1,280 ± 730 ml in need of a transfusion of 1,316 ± 874 ml due to moderate-to-severe adhesion and pretransplant pleurodesis. The causes of death of four patients (16%) included primary graft dysfunction, bronchial dehiscence with long-term use of sirolimus, and uncontrollable infections. The median follow-up time from LT was 41.1 ± 25.0 months. LT for LAM patients from the Asian population has been reinforced from the data that we presented. Peri-transplantation use of sirolimus and LAM-related complications should be further defined and under constant surveillance.
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http://dx.doi.org/10.3389/fmed.2021.584826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924661PMC
February 2021
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