Publications by authors named "Jian Liu"

3,498 Publications

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Dendrocrepidamine, a novel octahydroindolizine alkaloid from the roots of .

J Asian Nat Prod Res 2021 Jun 15:1-8. Epub 2021 Jun 15.

School of Traditional Chinese Medicines, China Pharmaceutical University, Nanjing 211198, China.

A novel octahydroindolizine alkaloid, named dendrocrepidamine () with an unusual 18,19,19'-cyclopropanone-dendrocrepine skeleton, was isolated from the ethanol extract of the roots of , along with six known compounds (-). The structure of was elucidated through HR-ESIMS, NMR spectroscopic data and computational calculations. All compounds were examined for their inhibitory effects on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in RAW264.7 cells with IC values in the range of 3.04-54.89 µM. , crepidatin () (80, 40 and 10 mg/kg) showed a significant protective effect against LPS-induced acute lung injury (ALI) in mice.
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http://dx.doi.org/10.1080/10286020.2021.1935891DOI Listing
June 2021

Development of a Healthy Assessment System For Residential Building Epidemic Prevention.

Build Environ 2021 Sep 10;202:108038. Epub 2021 Jun 10.

Ecological Technology Institute of Construction Engineering, Shenzhen University, Shenzhen, Guangdong, 518060, China.

During the period of COVID-19, the number of residents infected in urban communities continued to rise, implying that most of the current building layouts can't effectively resist the spread of infectious diseases, and the outbreak of COVID-19 has led to the need of changes for the current building environment. Therefore, the epidemic prevention should be considered in the residential building design, and the health design of residential community should be carried out from the perspective of epidemic prevention. In order to improve the ability of epidemic prevention of residential buildings and deal with the sudden pandemic and influenza in the post-epidemic era, () was developed according to the epidemic impact on residential buildings, the design and measures of epidemic prevention for residential buildings and the Chinese . Both entropy weight method and expert scoring method were used to determine the specific weight of the index. The HASRBEP includes control item assessment, preliminary assessment and extension assessment. The newly developed HASRBEP was used to assess the residential buildings of the Yulongzhuang Building Community located in Quanzhou, Fujian Province, China. The results show that the HASRBEP can be used to guide the health and epidemic prevention design of residential buildings.
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http://dx.doi.org/10.1016/j.buildenv.2021.108038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189736PMC
September 2021

Comparison of optical coherence tomography-guided and intravascular ultrasound-guided rotational atherectomy for calcified coronary lesions.

BMC Cardiovasc Disord 2021 Jun 11;21(1):290. Epub 2021 Jun 11.

Department of Cardiology, Peking University People's Hospital, NO.11 Xizhimen South Street, Xicheng District, Beijing, 100044, China.

Background: To compare the effect and outcomes of optical coherence tomography (OCT)-guided rotational atherectomy (RA) with intravascular ultrasound (IVUS)-guided RA in the treatment of calcified coronary lesions.

Methods: Data of calcified coronary lesions treated with RA that underwent OCT-guided or IVUS-guided from January 2016 to December 2019 at a single-center registry were retrospectively analyzed. The effect and outcomes between underwent OCT-guided RA and IVUS-guided RA were compared.

Results: A total of 33 lesions in 32 patients received OCT-guided RA and 51 lesions in 47 patients received IVUS-guided RA. There was no significant difference between OCT-guided RA group and IVUS-guided RA group in clinical baselines characteristics. Comparing the procedural and lesions characteristics of the two groups, the contrast volume was larger [(348.8 ± 110.6) ml vs. (275.2 ± 76.8) ml, P = 0.002] and the scoring balloon was more frequently performed (33.3% vs. 3.9%, P = 0.001) after RA and before stenting in the OCT-guided RA group. Comparing the intravascular imaging findings of the two groups, stent expansion was significantly larger in the OCT-guided RA group ([82 ± 8]% vs. [75 ± 9]%, P = 0.001). Both groups achieved procedural success immediately. There were no significantly differences in the incidence of complications. Although there was no statistical difference in the occurrence of MACE at 1 year between OCT-guided RA group and IVUS-guided RA group (3.1% vs. 6.4%, P = 0.517), no cardiovascular death, TVR and stent thrombosis occurred in OCT-guided RA group.

Conclusions: OCT-guided RA compared to IVUS-guided RA for treating calcified coronary lesions resulted in better stent expansion and may have improved prognosis.
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http://dx.doi.org/10.1186/s12872-021-02103-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193877PMC
June 2021

Traditional Chinese Medicine Qingre Huoxue Treatment vs. the Combination of Methotrexate and Hydroxychloroquine for Active Rheumatoid Arthritis: A Multicenter, Double-Blind, Randomized Controlled Trial.

Front Pharmacol 2021 25;12:679588. Epub 2021 May 25.

Guang'anmen Hospital China Academy of Chinese Medical Sciences, Beijing, China.

Traditional Chinese medicine (TCM) has been used successfully to treat rheumatoid arthritis (RA). Qingre Huoxue treatment (Qingre Huoxue decoction (QRHXD)/Qingre Huoxue external preparation (QRHXEP)) is a therapeutic scheme of TCM for RA. To date, there have been few studies comparing the efficacy and safety of QRHXD and conventional synthetic disease-modifying antirheumatic drugs (csDMARDs) for the treatment of active RA. This was investigated in a multicenter, double-blind, randomized controlled trial involving 468 Chinese patients with active RA [disease activity score (DAS)-28 > 3.2] treated with QRHXD/QRHXEP (TCM group), methotrexate plus hydroxychloroquine [Western medicine (WM) group], or both [integrative medicine (IM) group]. Patients were followed up for 24 weeks. The primary outcome measure was the change in DAS-28 from baseline to 24 weeks. The secondary outcome measures were treatment response rate according to American College of Rheumatology 20, 50, and 70% improvement criteria (ACR-20/50/70) and the rate of treatment-related adverse events (TRAEs). The trial was registered at ClinicalTrials.gov (NCT02551575). DAS-28 decreased in all three groups after treatment ( < 0.0001); the score was lowest in the TCM group ( < 0.05), while no difference was observed between the WM and IM groups ( > 0.05). At week 24, ACR-20 response was 73.04% with TCM, 80.17% with WM, and 73.95% with IM (based on the full analysis set [FAS], > 0.05); ACR-50 responses were 40.87, 47.93, and 51.26%, respectively, (FAS, > 0.05); and ACR-70 responses were 20.87, 22.31, and 25.21%, respectively, (FAS, > 0.05). Thus, treatment efficacy was similar across groups based on ACR criteria. On the other hand, the rate of TRAEs was significantly lower in the TCM group compared to the other groups ( < 0.05). Thus, QRHXD/QRHXEP was effective in alleviating the symptoms of active RA-albeit to a lesser degree than csDMARDs-with fewer side effects. Importantly, combination with QRHXD enhanced the efficacy of csDMARDs. These results provide evidence that QRHXD can be used as an adjunct to csDMARDs for the management of RA, especially in patients who experience TRAEs with standard drugs. Clinical Trial Registration: ClinicalTrials.gov, identifier NCTNCT025515.
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http://dx.doi.org/10.3389/fphar.2021.679588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186316PMC
May 2021

Graphene Oxide Functionalized Double-Layered Patch with Anti-Adhesion Ability for Abdominal Wall Defects.

Int J Nanomedicine 2021 3;16:3803-3818. Epub 2021 Jun 3.

Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China.

Background: Effective repair of full-thickness abdominal wall defects requires a patch with sufficient mechanical strength and anti-adhesion characteristics to avoid the formation of hernias and intra-abdominal complications such as intestinal obstruction and fistula. However, patches made from polymers or bio-derived materials may not meet these requirements and lack the bionic characteristics of the abdominal wall.

Materials And Methods: In this study, we report a consecutive electrospun method for preparing a double-layer structured nanofiber membrane (GO-PCL/CS-PCL) using polycaprolactone (PCL), graphene oxide (GO) and chitosan (CS). To expand the bio-functions (angiogenesis/reducing reactive oxygen species) of the patch (GO-PCL/NAC-CS-PCL), N-acetylcysteine (NAC) was loaded for the repair of full-thickness abdominal wall defects (2×1.5cm) in rat model.

Results: The double-layered patch (GO-PCL/NAC-CS-PCL) showed excellent mechanical strength and biocompatibility. After 2 months, rats treated with the patch exhibited the desired repair effect with no hernia formation, less adhesion (adhesion score: 1.50±0.50, P<0.001) and more collagen deposition (percentage of collagen deposition: 34.94%±3.31%, P<0.001).

Conclusion: The double-layered nanomembranes presented in this study have good anti-hernia and anti-adhesion effects, as well as improve the microenvironment in vivo. It, therefore, holds good prospects for the repair of abdominal wall defects and provides a promising key as a postoperative anti-adhesion agent.
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http://dx.doi.org/10.2147/IJN.S312074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184254PMC
June 2021

Nano-cell and nano-pollutant interactions constitute key elements in nanoparticle-pollutant combined cytotoxicity.

J Hazard Mater 2021 Jun 2;418:126259. Epub 2021 Jun 2.

School of Environmental Science and Engineering, Shandong University, Jinan, Shandong 250100, China; Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Institute of Environmental Research at Greater Bay, Guangzhou University, Guangzhou, Guangdong 510006, China. Electronic address:

As the wide application of carbon nanoparticles (CNPs) and zinc oxide nanoparticles (ZnONPs), as well as ubiquitous chromium (Cr(VI)) pollution in environment, the chance of human exposure to CNPs/ZnONPs and their Cr(VI) adducts is enhanced. We therefore investigated the impacts of nano-cell and nano-Cr(VI) interactions on nanoparticle-Cr(VI) combined cytotoxicity in human lung epithelial (A549) cells. Our results showed that nano-cell and nano-pollutant interactions were the key elements in NP-pollutant combined cytotoxicity, as determined by cell death, oxidative stress and mitochondrial dysfunction. A strong adsorption of Cr(VI) on CNPs and reduction of Cr(VI) to Cr(III) were confirmed, resulting in the reduced cytotoxicity of CNP-Cr(VI) adducts. In contrast, ZnONPs caused the destruction of cell membranes so that more ZnONP-Cr(VI) adducts could enter the cells. Meantime, more Cr contents could be released from ZnONP-Cr(VI) adducts once entering cells and locating in lysosomes than that from CNP-Cr(VI) adducts. These two reasons together caused the enhanced cytotoxicity of ZnONP-Cr(VI) adducts. These findings indicate that the in-depth investigations on the interaction mechanisms are crucial to comprehensively understand the combined cytotoxicity of different NPs and pollutants.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126259DOI Listing
June 2021

LncRNA JPX regulates proliferation and apoptosis of nucleus pulposus cells by targeting the miR-18a-5p/HIF-1α/Hippo-YAP pathway.

Biochem Biophys Res Commun 2021 Jun 7;566:16-23. Epub 2021 Jun 7.

Departments of Spine Surgery and Sports Medicine, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Xiuying, Haikou, Hainan, 570311, PR China. Electronic address:

With the aggravation of global aging, the rapid rise in the obesity rate, and the increasing number of patients with intervertebral disc degeneration (IDD), the principles and mechanism of this disease remain unclear. This study explored the molecular mechanism of IDD treatment through interactions of the lncRNA-miRNA-mRNA-signaling pathways and the effects on the proliferation and apoptosis of human nucleus pulposus cells (HNPCs) cultured in vitro. Our study revealed that lncRNA JPX is expressed at low levels in HNPCs under normoxic conditions. Luciferase and RNA pull-down assays were used to verify that lncRNA JPX directly bound to miR-18a-5p and influenced HNPC proliferation and apoptosis. Subsequently, a luciferase assay confirmed the direct binding of miR-18a-5p to HIF-1α and demonstrated a negative correlation between miR-18a-5p and HIF-1α. In addition, the HIF-1α antagonist reversed the inhibition of the Hippo-YAP pathway by the miR-18a-5p inhibitor. In conclusion, overexpression of lncRNA JPX upregulated HIF-1α by inhibiting the expression of miR-18a-5p, thereby inhibiting the Hippo-YAP pathway. By inhibiting this pathway, JPX overexpression promoted the proliferation of HNPCs and decreased their apoptosis. Therefore, the lncRNA JPX is a potential new target.
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http://dx.doi.org/10.1016/j.bbrc.2021.05.075DOI Listing
June 2021

Anti-HSP47 siRNA lipid nanoparticle ND-L02-s0201 reverses interstitial pulmonary fibrosis in preclinical rat models.

ERJ Open Res 2021 Apr 7;7(2). Epub 2021 Jun 7.

Nitto Biopharma Inc., San Diego, CA, USA.

ND-L02-s0201 is a lipid nanoparticle encapsulating an siRNA which inhibits expression of heat shock protein 47 (HSP47), a collagen-specific chaperone. Accumulated evidence demonstrates a close association between increased level of HSP47 and excessive accumulation of collagen in fibrotic diseases. Our objective was to test ND-L02-s0201 efficacy in preclinical lung fibrosis models and characterise the downstream histological and functional consequences of inhibiting the expression of HSP47. Comprehensive optimisation and characterisation of bleomycin (BLM) and silica-induced rat lung fibrosis models were conducted, which ensured progressive pathological changes were sustained throughout the study during evaluation of the anti-fibrotic potential of ND-L02-s0201. In the BLM model, we demonstrated dose-dependent and statistically significant reduction in the relative lung weight, collagen deposition and histology, and fibrosis scores following ND-L02-s0201 treatment. Lung tissue mRNA profiling demonstrated that 11 out of 84 fibrosis-relevant genes were upregulated following BLM induction and were downregulated by approximately 4.5-fold following ND-L02-s0201 treatment. Epithelial-mesenchymal transition was characterised in the BLM model following ND-L02-s0201 treatment. Cell enrichment demonstrated that myofibroblasts contained the highest HSP47 mRNA expression. BLM led to more than a five-fold increase in myofibroblasts and ND-L02-s0201 treatment reduced the myofibroblasts to sham levels. Statistically significant improvement in lung function was noted in the BLM model which was determined by running endurance capacity using a 7-minute treadmill test. Comparable anti-fibrotic efficacy was also observed in the silica model. Results from two robust chronic rodent models of pulmonary fibrosis demonstrated significant anti-fibrotic effects and improved lung function which support the evaluation of ND-L02-s0201 in subjects with idiopathic pulmonary fibrosis.
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http://dx.doi.org/10.1183/23120541.00733-2020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181707PMC
April 2021

Visceral-to-Subcutaneous Fat Ratio Is a Potential Predictor of Postoperative Complications in Colorectal Cancer.

Med Sci Monit 2021 Jun 8;27:e930329. Epub 2021 Jun 8.

Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China (mainland).

BACKGROUND Colorectal cancer (CRC) is one of the most common malignant tumors. Surgery is the primary treatment for CRC. Recent studies have shown that visceral-to-subcutaneous fat ratio (VSR) may be a potential indicator of risk. The aim of the present study was to determine whether VSR is a suitable predictor of the impact of postoperative complications in CRC. MATERIAL AND METHODS Clinical data from 129 patients were analyzed retrospectively. All patients underwent laparoscopic surgery for CRC. Preoperative imaging was used to quantify VSR. The primary outcome was 30-day postoperative complications. Multivariate logistic regression was used to determine the association between obesity indexes and postoperative complications. RESULTS There were 129 patients who underwent primary resections of colorectal cancer. At 30 days after surgery, postoperative complications had occurred in 33 (25.6%) patients. VSR was significantly associated with postoperative complications in multivariate analysis (P=0.032, OR 6.103, 95% CI 1.173-31.748). In ROC analysis, VSR was a potential predictor of complications (AUC 0.650). A cutoff value of VSR ≥0.707 was associated with 60% sensitivity and 29% specificity for postoperative complications. Patients with VSR ≥0.707 had 41.7% risk of morbidity, whereas those with <0.707 had 16.0% risk (P=0.001). CONCLUSIONS Our study shows that VSR is superior to VFA for prediction of the risk of complications following CRC laparoscopic surgery. We have also identified the optimal cutoff values for the use of VSR for this purpose. Measurement of VSR and identifying patients with increased risk of postoperative complications facilitate making perioperative decisions.
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http://dx.doi.org/10.12659/MSM.930329DOI Listing
June 2021

ZSCAN16-AS1 expedites hepatocellular carcinoma progression via modulating the miR-181c-5p/SPAG9 axis to activate the JNK pathway.

Cell Cycle 2021 Jun 7:1-13. Epub 2021 Jun 7.

Interventional Department, Henan Provincial People's Hospital (People's Hospital of Zhengzhou University, People's Hospital of Henan University), Zhengzhou, Henan, China.

Hepatocellular carcinoma (HCC) is generally known as one of the most common cancers in the world. Nowadays, interventional therapies such as transcatheter arterial chemoembolization (TACE) have emerged as an efficient therapy for HCC patients. Accumulating evidence has unveiled that long non-coding RNAs (lncRNAs) are crucial regulators in HCC progression. Nonetheless, the biological function of lncRNA zinc finger and SCAN domain containing 16 antisense RNA 1 (ZSCAN16-AS1) in HCC has not been systematically clarified. RT-qPCR was used to test ZSCAN16-AS1 expression in HCC cells. The biological functions of RP11-757 G1.5 on HCC cell proliferation, migration, invasion and apoptosis were investigated by colony formation, EdU, CCK-8 and transwell assays, as well as flow cytometry analysis. RNA immunoprecipitation (RIP), RNA pull-down and luciferase reporter assays were utilized to explore the specific mechanism of ZSCAN16-AS1. ZSCAN16-AS1 was significantly up-regulated in HCC cells. ZSCAN16-AS1 silence inhibited HCC cell proliferation, migration and invasion, while it accelerated HCC cell apoptosis. ZSCAN16-AS1 worked as a competing endogenous RNA (ceRNA) to regulate sperm associated antigen 9 (SPAG9) expression through sponging miR-181 c-5p. Moreover, SPAG9 could activate the c-Jun-N-terminal kinase (JNK) pathway. Taken together, our study elucidated that ZSCAN16-AS1 expedited HCC progression via modulating the miR-181 c-5p/SPAG9 axis to activate the JNK pathway, which might be a highly potential HCC therapy and treatment target.
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http://dx.doi.org/10.1080/15384101.2021.1919828DOI Listing
June 2021

The Sulfation Code of Tauopathies: Heparan Sulfate Proteoglycans in the Prion Like Spread of Tau Pathology.

Front Mol Biosci 2021 20;8:671458. Epub 2021 May 20.

Department of Biological Sciences, Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, United States.

Tauopathies are a heterogenous family of progressive neurodegenerative diseases defined by the appearance of proteinaceous lesions within the brain composed of abnormally folded species of Microtubule Associated Protein Tau (tau). Alzheimer's Disease (AD), the most common tauopathy, is the leading cause of cognitive decline among the elderly and is responsible for more than half of all cases of senile dementia worldwide. The characteristic pathology of many tauopathies-AD included-presents as Neurofibrillary Tangles (NFTs), insoluble inclusions found within the neurons of the central nervous system composed primarily of tau protein arranged into Paired Helical Fibrils (PHFs). The spatial extent of this pathology evolves in a remarkably consistent pattern over the course of disease progression. Among the leading hypotheses which seek to explain the stereotypical progression of tauopathies is the , which proposes that the spread of tau pathology is mediated by the transmission of self-propagating tau conformers between cells in a fashion analogous to the mechanism of communicable prion diseases. Protein-glycan interactions between tau and Heparan Sulfate Proteoglycans (HSPGs) have been implicated as a key facilitator in each stage of the prion-like propagation of tau pathology, from the initial secretion of intracellular tau protein into the extracellular matrix, to the uptake of pathogenic tau seeds by cells, and the self-assembly of tau into higher order aggregates. In this review we outline the biochemical basis of the tau-HS interaction and discuss our current understanding of the mechanisms by which these interactions contribute to the propagation of tau pathology in tauopathies, with a particular focus on AD.
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http://dx.doi.org/10.3389/fmolb.2021.671458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173255PMC
May 2021

Chemical synthesis of human syndecan-4 glycopeptide bearing O-, N-sulfation and multiple aspartic acids for probing impacts of the glycan chain and the core peptide on biological functions.

Chem Sci 2020 May 11;11(25):6393-6404. Epub 2020 May 11.

Department of Chemistry, Michigan State University 578 South Shaw Lane East Lansing MI 48824 USA.

Proteoglycans are a family of complex glycoproteins with glycosaminoglycan chains such as heparan sulfate (HS) attached to the core protein backbone. Due to the high structural heterogeneity of HS in nature, it is challenging to decipher the respective roles of the HS chain and the core protein on proteoglycan functions. While the sulfation patterns of HS dictate many activities, the core protein can potentially impact HS functions. In order to decipher this, homogeneous proteoglycan glycopeptides are needed. Herein, we report the first successful synthesis of proteoglycan glycopeptides bearing multiple aspartic acids in the core peptide and O- and N-sulfations in the glycan chain, as exemplified by the syndecan-4 glycopeptides. To overcome the high acid sensitivities of sulfates and base sensitivities of the glycopeptide during synthesis, a new synthetic approach has been developed to produce a sulfated glycan chain on a peptide sequence prone to the formation of aspartimide side products. The availability of the structurally well-defined synthetic glycopeptide enabled the investigation of their biological functions including cytokine, growth factor binding and heparanase inhibition. Interestingly, the glycopeptide exhibited context dependent enhancement or decrease of biological activities compared to the peptide or the glycan alone. The results presented herein suggest that besides varying the sulfation patterns of HS, linking the HS chain to core proteins as in proteoglycans may be an additional approach to modulate biological functions of HS in nature.
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http://dx.doi.org/10.1039/d0sc01140aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159385PMC
May 2020

Discovery of macrocyclic HDACs 1, 2, and 3 selective inhibitors for HIV latency reactivation.

Bioorg Med Chem Lett 2021 Jun 3:128168. Epub 2021 Jun 3.

Merck & Co., Inc., 2000 Galloping Hill Road, Kenilworth, NJ 07033, USA.

A series of unique macrocyclic HDACs 1, 2, and 3 selective inhibitors were identified with good enzymatic activity and high selectivity over HDACs 6 and 8. These macrocyclic HDAC inhibitors used an ethyl ketone as the zinc-binding group. Compounds 25 and 26 stood out as leads due to their low double-digit nM ECs in the 2C4 cell-based HIV latency reactivation assay. The PK profiles of these macrocyclic HDAC inhibitors still needed improvement.
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http://dx.doi.org/10.1016/j.bmcl.2021.128168DOI Listing
June 2021

Prolonged exposure to the herbicide atrazine suppresses immune cell functions by inducing spleen cell apoptosis in rats.

Ecotoxicol Environ Saf 2021 Sep 2;220:112386. Epub 2021 Jun 2.

School of Nursing, Jilin University, Changchun 130021, Jilin, China. Electronic address:

Atrazine (ATR) is a herbicide used widely worldwide. Because of its prolonged persistence in the environment and accumulation in the body, ATR exposure is a potential threat to human health. Our previous study showed that subacute exposure to ATR suppresses cellular immune function in mice. In this study, the effects of long-term exposure to ATR on rat immunological system function were measured. Four-week-old female Sprague-Dawley (SD) rats were treated with 0.4 μmol/L, 2 μmol/L and 10 μmol/L ATR for 24 weeks. The results showed that the spleen index increased, white blood cells decreased, and monocytes and eosinophils increased. No obvious changes were detected in the numbers of neutrophils and lymphocytes. Th1, Th2, and Th17 cells decreased significantly, while Treg cells increased after long-term ATR exposure. Moreover, serum levels of cytokines, including TNF-α, INF-γ, IL-6, and IL-12, decreased, while IL-1, IL-4, and IL-5 increased. Degenerative changes and cell apoptosis were found in the spleen; Caspase-3 and Caspase-9 were upregulated, and Bcl-2 was downregulated. These results suggested that long-term ATR exposure may inhibit immune system function.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112386DOI Listing
September 2021

['s experiences in treatment of diabetic retinopathy with acupuncture].

Zhongguo Zhen Jiu 2021 Jun;41(6):659-62

Shanhai Chinese Medicine Literature Museum.

, the chief physician, believes that diabetic retinopathy is located at eye and closely related to and . He proposes the treatment principle of this disease, i.e. combination of the primary and the secondary, in which, taking the eye as the treatment target and focusing on the symptoms. Acupuncture is provided for activating blood circulation and resolving stasis at the extra points, e.g. Xinming and Shangjianming, and also meridian points, e.g. Cuanzhu (BL 2) and Tongziliao (GB 1). The comprehensive application is emphasized with filiform needle, dermal needle and acupoint injection. Moreover, the mental and physical conditions are treated simultaneously to regulate emotions and the preventive idea is suggested on early treatment and persistent treatment.
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http://dx.doi.org/10.13703/j.0255-2930.20200603-k0001DOI Listing
June 2021

Transcriptome analysis of under benomyl stress to discriminate the gene expression in response to development and immune systems.

J Environ Sci Health B 2021 Jun 3:1-12. Epub 2021 Jun 3.

Sericultural Research Institute, Anhui Academy of Agricultural Sciences, Hefei, China.

The health and safety of the honeybees are seriously threatened due to the abuse of chemical pesticides in modern agriculture and apiculture. In this study, the RNA Seq approach was used to assess the effects of the honeybees treated with benomyl. The results showed that there were a total of 11,902 differentially expressed genes (DEGs). Among them, 5,759 DEGs were up-regulated and involved in the functions of immunity, detoxification, biological metabolism, and regulation. The DEGs were clustered in the GO terms of epidermal structure and response to external stimuli, and most of the DEGs were enriched in 15 pathways, such as light conduction, MAPK, calcium ion pathway, and so on. Moreover, the pathway of the toll signal transduction was activated. The data investigated that the expression of functional genes involved in the growth, development, foraging, and immunity of honeybees were significantly affected by benomyl stress, which would seriously threaten the health of the honeybees. This study provided a theoretical basis for revealing the response mechanism of honeybees to pesticides stress.
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http://dx.doi.org/10.1080/03601234.2021.1930795DOI Listing
June 2021

Lesions of the lateral habenula produce anxiolytic effects in a rat model of Parkinson's disease.

Neurol Res 2021 Jun 3:1-8. Epub 2021 Jun 3.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China.

: This study was designed to investigate the effects of lateral habenula (LHb) lesion on anxiety-like behaviors in parkinsonian rats.: Anxiety-like behaviors were assessed by the open field and elevated plus maze (EPM) tests in control, medial forebrain bundle (MFB)-lesioned, MFB- and LHb-lesioned and MFB-lesioned and LHb sham-lesioned rats, respectively. The levels of extracellular dopamine (DA), serotonin (5-HT) and noradrenaline (NA) in the basolateral amygdala (BLA) were measured by microdialysis and neurochemistry.: Compared to control rats, MFB lesions in rats decreased the percentage of time spent in the central area in the open field test and the percentages of open arm time and open arm entries in the EPM test, indicating the induction of anxiety-like behaviors, and this lesion also decreased the level of extracellular DA in the BLA. Further, rats in the MFB + LHb lesion group showed increased percentage of time spent in the central area and the percentages of open arm time and open arm entries compared to rats in the MFB lesion group, suggesting anxiolytic effects after lesioning the LHb. Neurochemical results found that lesions of the LHb increased the levels of extracellular DA and 5-HT in the BLA in the MFB and LHb lesion groups, whereas NA level was not altered.: These findings suggest that depletion of DA plays an important role in anxiety-like behaviors, and lesions of the LHb produce anxiolytic responses in MFB-lesioned rats, which are related to increased levels of extracellular DA and 5-HT in the BLA.
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http://dx.doi.org/10.1080/01616412.2021.1935100DOI Listing
June 2021

Genetic variants of  and ischemic clinical outcomes in coronary artery disease patients: a systematic review and meta-analysis.

Pharmacogenomics 2021 Jun 2. Epub 2021 Jun 2.

Department of Pharmacy Administration & Clinical Pharmacy, School of Pharmaceutical Science, Peking University, Beijing, 100191, China.

The aim of this study was to assess the association between  polymorphisms and ischemic clinical outcomes. We searched the electronic database for articles on the relationship of SNPs and ischemic events in patients with coronary artery disease (CAD) up to October 2020. A total of 9914 patients with CAD from six studies focusing on 12 SNPs of PEAR1 were included in this study. The A allele of rs12041331 were associated with ischemic events (odds ratio: 1.40; 95% CI: 1.04-1.88; p = 0.03). The AA homozygotes of rs2768759 was related to a higher risk of ischemic events than carriers of the C allele (odds ratio: 2.08; 95% CI: 1.09-3.97; p = 0.03). PEAR1 rs12041331 and rs2768759 are significantly associated with ischemic events in patients with CAD.
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http://dx.doi.org/10.2217/pgs-2021-0022DOI Listing
June 2021

Developing a Subjective Evaluation Scale for Assessing the Built Environments of China's Hygienic City Initiative.

Biomed Environ Sci 2021 May;34(5):372-378

Office of Epidemiology, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Objective: To develop a preliminary subjective evaluation scale for assessing the built environments of China's Hygienic City Initiative and to evaluate its reliability and validity.

Methods: The initial items of the scale were determined based on a review of policy documents and consultations with experts. The final items of the scale were confirmed through individual interviews with residents combined with the discretetrend method, critical ratio method, correlation coefficient method, and factor analysis method. Then, the dimensions of the scale were determined using exploratory factor analysis (EFA). The Cronbach's coefficient, split-half reliability coefficient, and confirmatory factor analysis (CFA) were used to assess the reliability and validity of the scale.

Results: A scale containing five dimensions with 22 items was established, including urban lifestyle, governance, basic functions, environmental sanitation, and amenities. The Cronbach's coefficient of the scale was 0.876, and the split-half reliability coefficient was 0.796. The CFA results indicate that each inspection level was within the standard limit.

Conclusion: The preliminarily subjective evaluation scale for assessing the built environments of China's Hygienic City Initiative demonstrates a high level of reliability and validity. Additional empirical studies should be carried out to further verify the value of the scale in terms of practical application.
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http://dx.doi.org/10.3967/bes2021.049DOI Listing
May 2021

Dynamics-Based Discovery of Novel, Potent Benzoic Acid Derivatives as Orally Bioavailable Selective Estrogen Receptor Degraders for ERα+ Breast Cancer.

J Med Chem 2021 06 31;64(11):7575-7595. Epub 2021 May 31.

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.

The estrogen receptor α (ERα) is identified as an effective target for the treatment of ERα+ breast cancer; thus, discovery of novel selective estrogen receptor degraders (SERDs) are developed as an effective method to overcome the resistance of breast cancer. Herein, the hot-spot residues for protein-ligand interaction between SERDs and ERα are analyzed by molecular dynamic simulation technology, focusing on the hot-spot residues for four series of designed and synthesized SERDs. SAR studies revealed that while the acrylic acid moiety of is scaffold hopping into benzoic acid, compound exhibits potent binding affinity with ERα, good degradation efficacy of ERα, and inhibitory effect against the MCF-7 breast cancer cell line. Besides, also displays good antitumor efficacy in the MCF-7 human breast cancer xenograft model in vivo, favorable pharmacokinetic properties, excellent druggability, and good safety property, making as a promising drug candidate of SERD for further evaluation.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00280DOI Listing
June 2021

Acer Truncatum Seed Oil Alleviates Learning and Memory Impairments of Aging Mice.

Front Cell Dev Biol 2021 14;9:680386. Epub 2021 May 14.

Key Laboratory of Ministry of Education of China and Hubei Province for Neurological Disorders, Department of Pathophysiology, School of Basic Medicine and the Collaborative Innovation Center for Brain Science, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Aging, characterized by a time-dependent functional decline of physiological integrity, is the major independent risk factor for many neurodegeneration diseases. Therefore, it's necessary to look for natural food supplements to extend the healthy lifespan of aging people. We here treated normal aging mice with acer truncatum seed oil, and found that the seed oil significantly improved the learning and memory ability. Proteomics revealed that the seed oil administration changed many proteins expression involving in biological processes, including complement and coagulation cascades, inflammatory response pathway and innate immune response. BDNF/TrkB signaling pathway was also activated by acer truncatum seed oil treatment. And the seed oil administration increased the expression of postsynaptic related proteins including PSD95, GluA1, and NMDAR1, and decreased the mRNA level of inflammatory factors containing IL-1β, TNF-α, and IL-6. These findings suggest that acer truncatum seed oil holds a promise as a therapeutic food supplement for delaying aging with multiple mechanisms.
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http://dx.doi.org/10.3389/fcell.2021.680386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160100PMC
May 2021

Controlling n-Type Molecular Doping Regiochemistry and Polarity of Pendant Groups on Low Band Gap Donor-Acceptor Copolymers.

Macromolecules 2021 Apr 8;54(8):3886-3896. Epub 2021 Apr 8.

Stratingh Institute for Chemistry, Nijenborgh 4, NL-9747 AG Groningen, The Netherlands.

We demonstrate the impact of the type and position of pendant groups on the n-doping of low-band gap donor-acceptor (D-A) copolymers. Polar glycol ether groups simultaneously increase the electron affinities of D-A copolymers and improve the host/dopant miscibility compared to nonpolar alkyl groups, improving the doping efficiency by a factor of over 40. The bulk mobility of the doped films increases with the fraction of polar groups, leading to a best conductivity of 0.08 S cm and power factor (PF) of 0.24 μW m K in the doped copolymer with the polar pendant groups on both the D and A moieties. We used spatially resolved absorption spectroscopy to relate commensurate morphological changes to the dispersion of dopants and to the relative local doping efficiency, demonstrating a direct relationship between the morphology of the polymer phase, the solvation of the molecular dopant, and the electrical properties of doped films. Our work offers fundamental new insights into the influence of the physical properties of pendant chains on the molecular doping process, which should be generalizable to any molecularly doped polymer films.
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http://dx.doi.org/10.1021/acs.macromol.1c00317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154869PMC
April 2021

Proteomics reveal biomethane production process induced by carbon nanotube.

Environ Res 2021 May 27;200:111417. Epub 2021 May 27.

Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China; CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China. Electronic address:

Biomethane produced by methanogenic archaea is a main greenhouse resource of terrestrial and marine ecosystems, which strongly affects the global environment change. Conductive materials, especially nano-scale, show considerable intervention on biomethane production potential, but the mechanism is still unclear. Herein, we precisely quantified the absolute abundance of Methanosarcina spp. proteins affected by carbon nanotubes (CNTs) using tandem mass tag (TMT) proteomics technology. Among the 927 detectable proteins, more than three hundred, 304, showed differential expression. Gene Set Enrichment Analysis on KEGG pathways and GO biological processes revealed a trend of decreased protein synthesis induced by CNTs, suggesting these conductive nanomaterials may replace part of the cell structure and function. Interestingly, increased acetoclastic methanogenesis actually came at the expense of reduced protein synthesis in related pathways. CNTs stimulated biomethane production from acetate by stimulating intracellular redox activity and the -COOH oxidation process. These findings enhanced the understanding of the biomethane production process affected by conductive materials.
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http://dx.doi.org/10.1016/j.envres.2021.111417DOI Listing
May 2021

Clinical report of a neonate carrying a large deletion in the 10p15.3p13 region and review of the literature.

Mol Cytogenet 2021 May 28;14(1):29. Epub 2021 May 28.

Department of Pediatrics, The First Affiliated Hospital of Fujian Medical University, Chazhong Road 20, Taijiang District, Fuzhou, 350004, Fujian, China.

Background: Terminal deletion of chromosome 10p is a rare chromosomal abnormality. We report a neonatal case with a large deletion of 10p15.3p13 diagnosed early because of severe clinical manifestations.

Case Presentation: Our patient presented with specific facial features, hypoparathyroidism, sen sorineural deafness, renal abnormalities, and developmental retardation, and carried a 12.6 Mb deletion in the 10p15.3 p13 region. The terminal 10p deletion involved in our patient is the second largest reported terminal deletion reported to date, and includes the ZMYND11 and GATA3 genes and a partial critical region of the DiGeorge syndrome 2 gene (DGS2).

Conclusion: On the basis of a literature review, this terminal 10p deletion in the present case is responsible for a specific contiguous gene syndrome. This rare case may help the understanding of the genotype-phenotype spectrum of terminal deletion of chromosome 10p.
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http://dx.doi.org/10.1186/s13039-021-00546-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164295PMC
May 2021

Laser-induced transient magnons in SrIrO throughout the Brillouin zone.

Proc Natl Acad Sci U S A 2021 Jun;118(22)

Condensed Matter Physics and Materials Science Department, Brookhaven National Laboratory, Upton, NY 11973.

Although ultrafast manipulation of magnetism holds great promise for new physical phenomena and applications, targeting specific states is held back by our limited understanding of how magnetic correlations evolve on ultrafast timescales. Using ultrafast resonant inelastic X-ray scattering we demonstrate that femtosecond laser pulses can excite transient magnons at large wavevectors in gapped antiferromagnets and that they persist for several picoseconds, which is opposite to what is observed in nearly gapless magnets. Our work suggests that materials with isotropic magnetic interactions are preferred to achieve rapid manipulation of magnetism.
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http://dx.doi.org/10.1073/pnas.2103696118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179144PMC
June 2021

Danggui Buxue Tang Rescues Folliculogenesis and Ovarian Cell Apoptosis in Rats with Premature Ovarian Insufficiency.

Evid Based Complement Alternat Med 2021 5;2021:6614302. Epub 2021 May 5.

Department of Gynaecology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Dade Road, Yuexiu District, Guangzhou 510120, Guangdong, China.

Premature ovarian insufficiency (POI) is a common female endocrine disease that is closely linked to ovarian function. Danggui Buxue Tang (DBT) is a classic prescription of traditional Chinese medicine that is helpful for rescuing ovarian function. However, the mechanism by which DBT rescues ovarian function remains unclear. This study explored the molecular mechanism of DBT with respect to apoptosis and related signals in ovarian cells. The quality control of DBT was performed by HPLC. After DBT intervention in the POI rat model, serum AMH/FSH/LH/E levels were detected by ELISA, follicles at various developmental stages were observed by HE staining, apoptosis was detected by TUNEL, and the expression profiles of Bcl-2 family proteins and key proteins in the Jak2/Foxo3a signaling pathway were evaluated by western blot. The results demonstrated that DBT could encourage the development of primary/secondary/antral follicles and increase the secretion of AMH. Moreover, DBT might inhibit Foxo3a by upregulating Jak2, thereby mediating Bcl-2 family activities and inhibiting apoptosis in ovarian cells. In conclusion, DBT promotes follicular development and rescues ovarian function by regulating Bcl-2 family proteins to inhibit cell apoptosis, which could be related to the Jak2/Foxo3a signaling pathway.
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http://dx.doi.org/10.1155/2021/6614302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8118728PMC
May 2021

Protein model accuracy estimation empowered by deep learning and inter-residue distance prediction in CASP14.

Sci Rep 2021 May 25;11(1):10943. Epub 2021 May 25.

Department of Electrical Engineering and Computer Science, University of Missouri-Columbia, Columbia, 65201, USA.

The inter-residue contact prediction and deep learning showed the promise to improve the estimation of protein model accuracy (EMA) in the 13th Critical Assessment of Protein Structure Prediction (CASP13). To further leverage the improved inter-residue distance predictions to enhance EMA, during the 2020 CASP14 experiment, we integrated several new inter-residue distance features with the existing model quality assessment features in several deep learning methods to predict the quality of protein structural models. According to the evaluation of performance in selecting the best model from the models of CASP14 targets, our three multi-model predictors of estimating model accuracy (MULTICOM-CONSTRUCT, MULTICOM-AI, and MULTICOM-CLUSTER) achieve the averaged loss of 0.073, 0.079, and 0.081, respectively, in terms of the global distance test score (GDT-TS). The three methods are ranked first, second, and third out of all 68 CASP14 predictors. MULTICOM-DEEP, the single-model predictor of estimating model accuracy (EMA), is ranked within top 10 among all the single-model EMA methods according to GDT-TS score loss. The results demonstrate that inter-residue distance features are valuable inputs for deep learning to predict the quality of protein structural models. However, larger training datasets and better ways of leveraging inter-residue distance information are needed to fully explore its potentials.
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http://dx.doi.org/10.1038/s41598-021-90303-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8149836PMC
May 2021

Low-dose theophylline in addition to ICS therapy in COPD patients: A systematic review and meta-analysis.

PLoS One 2021 24;16(5):e0251348. Epub 2021 May 24.

Department of Intensive Care Unit, Lanzhou University, The First Hospital of Lanzhou University, Lan Zhou, Gansu Province, China.

Background: A synergism has been reported between theophylline and corticosteroids, wherein theophylline increases and restores the anti-inflammatory effect of inhaled corticosteroids (ICS) by enhancing histone deacetylase-2 (HDAC) activity. Several studies have explored the efficacy of low-dose theophylline plus ICS therapy on chronic obstructive pulmonary disease (COPD) but the results are discrepant.

Method: We conducted searches in electronic database such as PubMed, Web Of Science, Cochrane Library, and Embase to find out original studies. Stata/SE 15.0 was used to perform all data analysis.

Result: A total of 47,556 participants from 7 studies were included in our analysis and the sample size of each study varied from 24 to 10,816. Theophylline as an add-on therapy to ICS was not associated with the reduction of COPD exacerbations (HR: 1.08, 95% CI: 0.97 to 1.19, I2 = 95.2%). Instead, the theophylline group demonstrated a higher hospitalization rate (HR: 1.12, 95% CI: 1.10 to 1.15, I2 = 20.4%) and mortality (HR: 1.19, 95% CI: 1.14 to 1.25, I2 = 0%). Further, the anti-inflammatory effect of low-dose theophylline as an adjunct to ICS on COPD was controversial. Besides, the theophylline group showed significant improvement in lung function compared with the non-theophylline group.

Conclusion: Based on current evidence, low-dose theophylline as add-on therapy to ICS did not reduce the exacerbation rate. Instead, the hospitalization rate and mortality increased with theophylline. Thus, we do not recommend adding low-dose theophylline to ICS therapy in COPD patients.

Trial Registration: PROSPERO Registration CRD42021224952.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251348PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143407PMC
May 2021

Chrysin Stimulates Subcutaneous Fat Thermogenesis in Mice by Regulating PDGFRα and MicroRNA Expressions.

J Agric Food Chem 2021 Jun 24;69(21):5897-5906. Epub 2021 May 24.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.

The activation of adipose tissue browning and thermogenesis provides a new strategy to counter obesity and associated metabolic diseases. Here, a natural flavonoid chrysin is used as the supplement of a high-fat diet (HFD). Dietary chrysin alleviates adiposity and insulin resistance in HFD-fed mice. Meanwhile, dietary chrysin elevates systemic energy expenditure and enhances the uncoupling protein-1 (UCP1) level in subcutaneous adipose tissue (SAT), which is accompanied by the increased thermogenic program, beige preadipocyte number, and angiogenesis in SAT. Dietary chrysin also induces the expression of SAT platelet-derived growth factor receptor α (PDGFRα), which commits adipose progenitor cells to differentiate into beige or white adipocytes in response to various environmental signals. Double immunofluorescent staining for UCP1 and PDGFRα reveals that chrysin elevates the number of UCP1PDGFRα beige progenitors in SAT. Further, chrysin treatment reverses the effects of the specific PDGFRα inhibitor imatinib on browning differentiation of stromal vascular fraction cells from SAT. Finally, chrysin-induced adipocyte browning is correlated with the expressions of microRNAs as PDGFRα inhibitors or thermogenesis suppressors. In conclusion, dietary chrysin promotes subcutaneous adipocyte browning and systematic energy expenditure by regulating PDGFRα and microRNA expressions in HFD-fed mice.
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http://dx.doi.org/10.1021/acs.jafc.1c01130DOI Listing
June 2021

Identification of Key Genes With Differential Correlations in Lung Adenocarcinoma.

Front Cell Dev Biol 2021 5;9:675438. Epub 2021 May 5.

Tumor Biological Diagnosis and Treatment Center, The Third Affiliated Hospital of Soochow University, Changzhou, China.

Background: With the advent of large-scale molecular profiling, an increasing number of oncogenic drivers contributing to precise medicine and reshaping classification of lung adenocarcinoma (LUAD) have been identified. However, only a minority of patients archived improved outcome under current standard therapies because of the dynamic mutational spectrum, which required expanding susceptible gene libraries. Accumulating evidence has witnessed that understanding gene regulatory networks as well as their changing processes was helpful in identifying core genes which acted as master regulators during carcinogenesis. The present study aimed at identifying key genes with differential correlations between normal and tumor status.

Methods: Weighted gene co-expression network analysis (WGCNA) was employed to build a gene interaction network using the expression profile of LUAD from The Cancer Genome Atlas (TCGA). R package DiffCorr was implemented for the identification of differential correlations between tumor and adjacent normal tissues. STRING and Cytoscape were used for the construction and visualization of biological networks.

Results: A total of 176 modules were detected in the network, among which yellow and medium orchid modules showed the most significant associations with LUAD. Then genes in these two modules were further chosen to evaluate their differential correlations. Finally, dozens of novel genes with opposite correlations including ATP13A4-AS1, HIGD1B, DAP3, and ISG20L2 were identified. Further biological and survival analyses highlighted their potential values in the diagnosis and treatment of LUAD. Moreover, real-time qPCR confirmed the expression patterns of ATP13A4-AS1, HIGD1B, DAP3, and ISG20L2 in LUAD tissues and cell lines.

Conclusion: Our study provided new insights into the gene regulatory mechanisms during transition from normal to tumor, pioneering a network-based algorithm in the application of tumor etiology.
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http://dx.doi.org/10.3389/fcell.2021.675438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131847PMC
May 2021