Publications by authors named "Jian Jiang"

486 Publications

The application of venous nerve conduit trap in the immediate repair and reconstruction of facial nerve in parotid gland tumor: an attempt of a new technique.

Eur Arch Otorhinolaryngol 2021 May 4. Epub 2021 May 4.

Department of Head and Neck Surgery, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology, Chengdu, China.

Objective: To introduce the application of venous nerve conduit in the immediate repair and reconstruction of facial nerve in parotid gland tumor.

Methods: Three patients with parotid gland tumor in Sichuan Provincial Cancer Hospital were reviewed. All patients were found that the tumor encased and invaded the facial nerve which was difficult to be separated during the operation when all patients were treated with facial nerve repair and reconstruction with the venous nerve conduit trapping technique.

Results: After 1-year follow-up, all patients recovered well in facial nerve function.

Conclusion: The venous nerve conduit trapping technique is an effective attempt in the immediate repair and reconstruction of facial nerve in parotid gland tumor, but it needs to be further confirmed by multiple studies.
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http://dx.doi.org/10.1007/s00405-021-06732-6DOI Listing
May 2021

Nonelectrostatic Adsorption of Polyelectrolytes and Mediated Interactions between Solid Surfaces.

Langmuir 2021 Apr 29. Epub 2021 Apr 29.

Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 E California Blvd, Pasadena, California 91125, United States.

Polymer-mediated interaction between two solid surfaces is directly connected to the properties of the adsorbed polymer layers. Nonelectrostatic interactions with a surface can significantly impact the adsorption of polyelectrolytes to charged surfaces. We use a classical density functional theory to study the effect of various polyelectrolyte solution properties on the adsorption and interaction between two like-charged surfaces. Our results show that nonelectrostatic interactions not only enhance polyelectrolyte adsorption but can also result in qualitatively different salt effects with respect to the adsorbed amount. In particular, we observe decreasing, increasing, and a previously unreported nonmonotonic behavior in the adsorbed amount of polymer with added salt under the conditions studied, although the nonmonotonic regime only occurs for a narrow range in the parameter space. With sufficient nonelectrostatic adsorption, the adsorbed polymer layers produce a long-range repulsive barrier that is strong enough to overcome dispersive interactions that cause surfaces to attract. Concurrently, a short-range bridging attraction is observed when the two polyelectrolyte layers span both the surfaces. Both the repulsive barrier and bridging attraction depend on the charge density of the polymer backbone and the bulk salt concentration but not on the chain length in the semidilute regime studied.
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http://dx.doi.org/10.1021/acs.langmuir.1c00139DOI Listing
April 2021

Inversing supramolecular chirality and boosting circularly polarized luminescence of pyrene moieties a gel matrix.

Soft Matter 2021 Apr;17(16):4328-4334

Key laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.

Alkyl-substituted l/d-glutamide derivatives (L/D-SG) were designed as gelators to fabricate host gel matrices. Pyrene-appended l/d-glutamide derivatives (Py-LG/Py-DG) were employed as guest luminophores to investigate chiral packing and emission behavior in gel matrices. It was found that Py-LG and Py-DG are prone to form P- and M-chirally packed assemblies in DMSO gels, respectively. However, the chiral packing was inversed, and CPL was boosted after Py-LG/Py-DG was embedded in the L/D-SG gel matrix. M-chiral packing together with left-handed excimer emission ((-)-CPL) was observed in the Py-LG immobilized L-SG gel matrix, while P-chiral packing together with right-handed excimer emission ((+)-CPL) was found in the Py-DG immobilized L-SG gel matrix. It is more interesting to find that the molecular chirality of the matrix gelator did not affect the supramolecular chirality of pyrene assemblies. Either l or a d-matrix gelator can inverse the supramolecular chirality of the pure gel, but did not follow the chirality of the matrix. It was found that the gel matrix converts intralayer pyrene-pyrene (Py-Py) packing in the pure pyrene gel to interlayer Py-Py packing, thus giving an opposite chirality. The study not only deepened our understanding of the supramolecular chirality transfer but also unveiled the effects of an inert gel matrix in regulating the chiroptical properties.
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http://dx.doi.org/10.1039/d1sm00262gDOI Listing
April 2021

The role of miRNA, lncRNA and circRNA in the development of intervertebral disk degeneration (Review).

Exp Ther Med 2021 Jun 26;21(6):555. Epub 2021 Mar 26.

Department of Oncology Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116000, P.R. China.

Intervertebral disc degeneration (IVDD) is a degenerative musculoskeletal disorder with multiple causative factors, such as age, genetics, mechanics and life style. IVDD contributes to non-specific lower back pain (NLBP), which is a globally prevalent and debilitating musculoskeletal disorder. NLBP has a substantial impact on medical resources and creates an economic burden for the public. Dysregulated phenotypes of nucleus pulposus (NP) cells and endplate chondrocytes, such as proliferation, senescence and apoptosis, along with aberrant expression of extracellular matrix components, including type II collagen and aggrecan, are involved in the pathological process of IVDD. Evidence indicates that non-coding RNAs, mainly microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), play a vital role in the development of IVDD. In the present review, the potential molecular mechanisms of miRNAs, lncRNAs and circRNAs in the initiation and progression of IVDD were described based on the latest literature. Furthermore, ways to influence the functions of NP cells and endplate chondrocytes in IVDD were also summarized. The presented insights suggested that non-coding RNAs may function as potential targets for the treatment of IVDD.
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http://dx.doi.org/10.3892/etm.2021.9987DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027750PMC
June 2021

Relationship between the extent of central node dissection and parathyroid function preservation in thyroid cancer surgery.

Gland Surg 2021 Mar;10(3):1093-1103

Department of Head and Neck Surgery, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Background: The risk and effect of hypocalcemia following surgery of different magnitudes remains unclear. Thus, we assessed whether different extents of central lymph node dissection (CLND) and status of preserved parathyroid glands can influence parathyroid function in differentiated thyroid carcinoma (DTC) patients with initial surgical resection.

Methods: Participants were categorized into 6 groups based on the extent of the surgical procedures, number of parathyroid glands preserved , and parathyroid autotransplantation. The frequencies of hypocalcemia, serum calcium (Ca) levels, and parathyroid hormone (PTH) levels among the different groups were analyzed.

Results: The prevalence of hypocalcemia, number and status of parathyroid glands preserved , and parathyroid autotransplantation were inversely related to extensive CLND (r=-0.18; P<0.05). The decrease of serum Ca and PTH was most severe on postoperative days (POD) 1-7. The incidence of hypocalcemia was higher in Group C than in Group B and A (P<0.05). The average postoperative serum Ca and PTH levels in Group C were significantly lower than group A on POD 1-7 (P<0.05). The incidence of hypocalcemia was obviously increased in Group D compared to Groups E and F (P<0.05). The mean serum Ca and PTH levels in Group D were significantly lower than in Group F (P<0.05), and the same results could be observed between Groups D and E (P<0.05). However, through parathyroid autotransplantation, there was no significant difference that could be found between Groups E and F on POD 1-7 (P>0.05).

Conclusions: With the expansion of CLND scope, postoperative parathyroid function will be affected, increasing the risk of postoperative hypocalcemia. When at least 1-2 parathyroid glands were reserved plus at least 1 parathyroid gland autotransplantation, there was little effect on postoperative parathyroid function.
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http://dx.doi.org/10.21037/gs-21-121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033073PMC
March 2021

Clinical characteristics and endovascular treatment in patients with intracranial giant serpentine aneurysms.

Quant Imaging Med Surg 2021 Apr;11(4):1490-1495

Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, China.

Background: Giant serpentine aneurysms (GSAs) are a rare subtype of intracranial aneurysm. Recently, GSAs have been successfully treated with endovascular parent artery occlusion with or without distal bypass. The present study retrospectively analyzed the clinical outcomes of endovascular parent artery occlusion for intracranial GSAs.

Methods: Medical records and cerebral angiograms from our endovascular center were retrospectively analyzed. Twenty-two patients with serpentine aneurysms were treated with endovascular occlusion of the parent artery at the site of the aneurysm. These patients had selective treatment. Clinical and angiographic outcomes of the patients were assessed between the 3-month to 3-year stage.

Results: The clinical manifestations of GSA included headache, hemiparesis, SAH, epilepsy, memory loss, right oculomotor palsy, and intracerebral hemorrhage. No cerebral infarction occurred. The average Glasgow Outcome Scale (GOS) score was 5. There were no clinical complications and none of the patients had recurrence after selective embolization. All of the patients recovered well, and no recurrence or rebleeding was noted at the follow-up.

Conclusions: Endovascular parent artery occlusion may be a safe and effective way to treat intracranial GSAs.
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http://dx.doi.org/10.21037/qims-20-698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930703PMC
April 2021

Experimental study on the characteristics of train-induced vibration in a new structure of metro depot.

Environ Sci Pollut Res Int 2021 Mar 30. Epub 2021 Mar 30.

School of Civil Engineering and Architecture, East China Jiaotong University, Nanchang, 330013, Jiangxi, China.

In order to improve the utilization rate of expensive and very limited urban land, a new structure system, underground metro depot, has been developed and applied in several cities in China. The underground metro depot, which is built underground, can be multiple stories, and allows other developments on top, has become increasingly popular in recent years. Since there are other developments on top, the vibration induced by the trains frequently entering and exiting the depot cannot be ignored. To better understand the characteristics of the train-induced vibration in the underground metro depot, a series of field tests were conducted on a two-story underground depot which is the largest underground metro depot in Asia. The results show that the main frequency components of vibration source are between 50 and 200 Hz and the level of that in the first floor underground is larger than that in the second floor underground due to the train floor structure interaction when the train running on the first floor underground. The vibration in the top platform decreases linearly with the distance from the measuring point to the center line of the track. When the train is running on the track at the edge area, the linear attenuation rate was about 0.2dB/m. It is easy to excite the vibration mode of the floor structure when the train is running on the first floor underground, so the vibration in the top platform induced by the train running on the first floor underground was much larger than the train running on the second floor underground. In the future design, if the underground depot has more than one floor, the structural dynamic behavior of over-track buildings induced by the train running on the floor slab underground should be the focus. The throat area has many rail joints and turnouts which can lead to a larger vibration level, the level easy to exceed the limit given for human vibration comfort. If developments are proposed on top of the throat area, the vibration level should be carefully checked in the area within 30 m from the track to avoid potential later vibration problem.
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http://dx.doi.org/10.1007/s11356-021-13599-xDOI Listing
March 2021

Revisiting the Charged Shell Model: A Density Functional Theory for Electrolytes.

J Chem Theory Comput 2021 Apr 30;17(4):2409-2416. Epub 2021 Mar 30.

Department of Physiology and Biophysics, Rush University Medical Center, Chicago, Illinois 60612, United States.

Classical density functional theory (DFT) has proven to be a sophisticated and efficient approach for investigating charge systems. In DFT, the excess free energy functional for inhomogeneous charged hard-sphere fluids consists of hard-core interactions and charge-charge electrostatic interactions. The former component can be precisely described by well-established fundamental measure theory (FMT). The latter component is usually computed using the Poisson equation combined with the mean spherical approximation (MSA). In order to predict accurate density profiles of ions and satisfy some thermodynamics sum rules, Roth and Gillespie [, , 244006] proposed a DFT combining a functional-based version of MSA and an approximated charged shell model. Here, we rebuild the DFT based on the exact charged shell model, and the analytic expressions for the shell interaction potential and the corresponding thermodynamic quantities are provided. The structural and thermodynamic properties of both bulk and inhomogeneous electrolyte systems are analyzed. Moreover, the software named (an advanced theoretical tool for inhomogeneous fluids) is released to the public via this work.
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http://dx.doi.org/10.1021/acs.jctc.1c00052DOI Listing
April 2021

miR-23a-3p regulates the proliferation and apoptosis of human lens epithelial cells by targeting Bcl-2 in an model of cataracts.

Exp Ther Med 2021 May 26;21(5):436. Epub 2021 Feb 26.

Department of Ophthalmology, Zhongshan Hospital Xiamen Branch, Fudan University, Xiamen, Fujian 361000, P.R. China.

Cataracts account for ~50% of the cases of blindness in individuals worldwide. The apoptosis of lens epithelial cells (LECs) occurs during the formation of cataracts, which is a non-congenital condition. Numerous microRNAs (miRs) have been reported to regulate apoptosis in LECs. For instance, miR-23a expression levels were shown to be upregulated in cataractous lenses; however, the function of miR-23a in cataracts remains undetermined. To establish an model of cataracts, human LECs, HLE-B3 cells, were induced with 200 µmol/l HO for 24 h. HLE-B3 cells were transfected with the miR-negative control (NC) mimic, miR-23a-3p mimic, miR-NC inhibitor, miR-23a-3p inhibitor, small interfering RNA (siRNA) targeting BCL2 (siRNA-BCL2) and siRNA-NC. The expression levels of miR-23a-3p were detected using reverse transcription-quantitative PCR. The interaction between miR-23a-3p and the 3'-untranslated region (UTR) of the target mRNA BCL2 was predicted by TargetScan 7.1, and further validated using a dual luciferase reporter assay. The BCL2 protein expression levels were analyzed using western blotting, cell proliferation was determined using a CCK-8 assay and the levels of cell apoptosis were analyzed using flow cytometric analysis. The results of the present study revealed that the expression levels of miR-23a-3p were significantly upregulated, while the expression levels of BCL2 were significantly downregulated in HO-induced HLE-B3 cells compared to untreated control cells. BCL2 was shown to be a target of miR-23a-3p. The miR-23a-3p inhibitor subsequently attenuated HO-induced apoptosis and increased the proliferation of HLE-B3 cells, which was partially reversed by siRNA-BCL2. In conclusion, the findings of the current study suggested that the inhibition of miR-23a-3p may attenuate HO-induced cataract formation by targeting BCL2, thus providing a novel therapeutic target for the treatment of patients with cataracts in the clinic.
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http://dx.doi.org/10.3892/etm.2021.9853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967796PMC
May 2021

Application of microlecture teaching methods in standardized residency training during COVID-19 in Wuhan: a randomized, controlled study.

Ann Palliat Med 2021 Mar 18. Epub 2021 Mar 18.

Department of Neurology, The First Hospital of Wuhan City, Wuhan, China.

Background: Providing high-quality training to residency students during the coronavirus 2019 (COVID-19) pandemic has been a goal of our institution. Since 2108, we began to take microlectures to students teaching. Microlectures are online presentations, and the microlecture teaching method has many advantages, such as a short teaching time, situational resource composition, diverse communication, strong pertinence and can attend microlectures from home. The aim of the present study was to evaluate the advantages of the microlecture teaching method on students in standardized residency training.

Methods: Students from our department were randomly divided into the traditional teaching group (control group) and the microlecture teaching group (observation group). The teaching duration for both groups was 3 months. All students were assessed on basic knowledge of the neurology before enrollment. After the teaching session, the students were assessed on teaching effect, theoretical operation, and clinical practice satisfaction. The students also evaluated the teachers, and the teachers evaluated the students.

Results: A total of 84 students participated in the study and were divided equally into the observation group (42 students) and the control group (42 students). The results showed that the rate of reaching the standard of teaching effect, achievement of theory and operation, satisfaction with clinical practice, the student's grades by teachers, and student satisfaction with teachers were significantly higher in the observation group than in the control group (all P<0.05).

Conclusions: The microlecture teaching method can effectively improve the clinical teaching effect for neurology students and should be adopted in clinical teaching, especially during the COVID-19 pandemic.
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http://dx.doi.org/10.21037/apm-21-22DOI Listing
March 2021

Fecal microbiota transplantation ameliorates gut microbiota imbalance and intestinal barrier damage in rats with stress-induced depressive-like behavior.

Eur J Neurosci 2021 Mar 20. Epub 2021 Mar 20.

Department of Public Health, Jining Medical University, Jining, China.

The gut-microbiota-brain axis is the most important complex and bidirectional pathway between the gastrointestinal tract and the central nervous system. This study investigated the potential of microbe-induced gut-to-brain signaling to modulate the effect of stress on depressive-like behavior, intestinal barrier, and neuroinflammation. Result showed that fecal microbiota transplantation increased the consumption of sucrose solutions and decreased the immobility time in forced swimming test. This treatment also increased Firmicutes and decreased Bacteroidetes and Desulfobacterota at phylum levels; reduced the loss of villi and epithelial cells; suppressed the inflammatory cell infiltration in ileum; increased the expression of ZO-1, occludin; protected the mucosal layer function; and suppressed the high levels of inflammasomes (NLRP3, ASC, caspase-1, and IL-1β) in rat brain. In summary, fecal microbiota transplantation improves the depressive-like behavior, alters the gut microbiota imbalance, and alleviates intestinal tract inflammation, intestinal mucosa disruption, and neuroinflammation in rats induced by chronic unpredictable mild stress.
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http://dx.doi.org/10.1111/ejn.15192DOI Listing
March 2021

GGL-Tox: Geometric Graph Learning for Toxicity Prediction.

J Chem Inf Model 2021 Apr 15;61(4):1691-1700. Epub 2021 Mar 15.

Department of Mathematics, Michigan State University, East Lansing, Michigan 48824, United States.

Toxicity analysis is a major challenge in drug design and discovery. Recently significant progress has been made through machine learning due to its accuracy, efficiency, and lower cost. US Toxicology in the 21st Century (Tox21) screened a large library of compounds, including approximately 12 000 environmental chemicals and drugs, for different mechanisms responsible for eliciting toxic effects. The Tox21 Data Challenge offered a platform to evaluate different computational methods for toxicity predictions. Inspired by the success of multiscale weighted colored graph (MWCG) theory in protein-ligand binding affinity predictions, we consider MWCG theory for toxicity analysis. In the present work, we develop a geometric graph learning toxicity (GGL-Tox) model by integrating MWCG features and the gradient boosting decision tree (GBDT) algorithm. The benchmark tests of the Tox21 Data Challenge are employed to demonstrate the utility and usefulness of the proposed GGL-Tox model. An extensive comparison with other state-of-the-art models indicates that GGL-Tox is an accurate and efficient model for toxicity analysis and prediction.
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http://dx.doi.org/10.1021/acs.jcim.0c01294DOI Listing
April 2021

Description of a new species of the genus Eaton, 1885 (Ephemeroptera, Ameletidae) from Yunnan, China.

Zookeys 2021 1;1021:37-51. Epub 2021 Mar 1.

Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China, Dali University, Dali, Yunnan, China South China Agricultural University Guangzhou China.

A new species with primitive characteristics, Tong, , is described, based on the morphology of imago, larva and egg with molecular data of the mitochondrial COI from Mount Cangshan, Dali, China. The new species is closely related to one of the most primitive mayflies, Traver, 1939, by sharing persistent mouthparts in the alate stage, but it can be distinguished from the latter by the morphological differences of the mouthpart remains, wings and genitals in the imaginal stage. Both morphological and molecular evidence support that Tong, is a new member of the genus . The discovery of the new species could help understand the origin and evolution of the genus .
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http://dx.doi.org/10.3897/zookeys.1021.59927DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940326PMC
March 2021

Retracted: Parthenolide Inhibits the Proliferation of MDA-T32 Papillary Thyroid Carcinoma Cells In Vitro and in Mouse Tumor Xenografts and Activates Autophagy and Apoptosis by Downregulation of the Mammalian Target of Rapamycin (mTOR)/PI3K/AKT Signaling Pathway.

Med Sci Monit 2021 Mar 11;27:e931025. Epub 2021 Mar 11.

Department of Head and Neck Surgery, Sichuan Provincial Cancer Hospital, Chengdu, Sichuan, China (mainland).

This paper is being retracted at the author's request. Reference: 1. Chao Li, Yuqiu Zhou, Yongcong Cai, Chunyan Shui, Wei Liu, Xu Wang, Jian Jiang, Dingfen Zeng, Chunhan Gui, Ronghao Sun: Parthenolide Inhibits the Proliferation of MDA-T32 Papillary Thyroid Carcinoma Cells In Vitro and in Mouse Tumor Xenografts and Activates Autophagy and Apoptosis by Downregulation of the Mammalian Target of Rapamycin (mTOR)/PI3K/AKT Signaling Pathway. Med Sci Monit, 2019; 25: 5054-5061. DOI: 10.12659/MSM.915387.
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http://dx.doi.org/10.12659/MSM.931025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7962496PMC
March 2021

Fecal microbiota transplantation ameliorates stress-induced depression-like behaviors associated with the inhibition of glial and NLRP3 inflammasome in rat brain.

J Psychiatr Res 2021 May 1;137:147-157. Epub 2021 Mar 1.

Department of Psychiatry, Huai'an Third People's Hospital, Huai'an, Jiangsu province,China; Department of Mental Health, Jining Medical University, Jining, China. Electronic address:

Background: Evidence from previous studies has demonstrated that the gut-microbiota-brain axis is vital in regulating of behavior and neuroinflammation in the central nervous system. Considering the putative connection among gut microbiota, neural function, and behavior, the present study investigated the potential signaling of gut microbiota to modulate depression-like behaviors and neuroinflammation.

Methods: Rats showing depression-like behaviors induced by chronic unpredictable mild stress received fecal microbiota treatment or vehicle for 14 days, and alterations in behavior and neuroinflammation were assessed. ELISA, immunofluorescence staining and Western blot were used to analysis the activation of glial cells and NLRP3 inflammasome.

Results: Treatment with fecal microbiota transplantation ameliorated depression-like behaviors. 5-Hydroxytryptamine decreased in the chronic unpredictable mild stress rat model but significantly increased after fecal microbiota transplantation. The treatment with fecal microbiota transplantation decreased the production of IL-1β and TNF-α. Moreover, fecal microbiota transplantation administration suppressed the activation of Iba1 positive microglia cells and GFAP positive astrocytes cells and reduced the expression of NLRP3, ASC, Caspase-1, and IL-1β pathway in the prefrontal cortex and hippocampus.

Conclusions: Fecal microbiota transplantation can improve depression-like behaviors induced by chronic unpredictable mild stress. The anti-depression effects of fecal microbiota transplantation were associated with the suppressed activation of glial cells and NLRP3 inflammasome in the brain.
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http://dx.doi.org/10.1016/j.jpsychires.2021.02.057DOI Listing
May 2021

Ultrasmall FeS Nanoparticles-Decorated Carbon Spheres with Laser-Mediated Ferrous Ion Release for Antibacterial Therapy.

Small 2021 Apr 4;17(13):e2005473. Epub 2021 Mar 4.

CAS Engineering Laboratory for Nanozyme, Key Laboratory of Protein and Peptide Pharmaceutical, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.

Recent progress in nanotechnology and the ancient use of sulfur in treating dermatological disorders have promoted the development of nano-sulfides for antimicrobial applications. However, the variable valences and abundant forms of nano-sulfides have complicated investigations on their antibacterial activity. Here, carbon nanospheres (CNSs) with decoration of ultrasmall FeS nanoparticles (CNSs@FeS ) is synthesized, and their antibacterial ability and mechanism are explored. The CNSs@FeS released Fe and sulfur ions simultaneously through dissolution and disproportionation. In vitro study indicated that the released Fe killed bacteria by increasing the oxidative state of bacterial surfaces and intracellular molecules. Importantly, the released sulfur exhibited a protective effect on Fe , ensuring the stable existence of Fe to continuously combat bacteria. Moreover, the carbon shells of CNSs@FeS not only prevented the aggregation of FeS but also accelerated the release of Fe through photothermal effects to achieve synergistic hyperthermia/Fe therapy. In vivo experiments indicated that treatment with CNSs@FeS resulted in a marked reduction in bacterial number and improvement in survival in an acute peritonitis mouse model, and antibacterial wound experiments demonstrated high efficacy of CNSs@FeS -enabled synergistic hyperthermia/Fe therapy. Thus, this study clarifies the antibacterial mechanism of FeS and offers a synergetic therapeutic platform with laser-mediated Fe release for antibacterial applications.
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http://dx.doi.org/10.1002/smll.202005473DOI Listing
April 2021

Pharmacokinetics-based comprehensive strategy to identify multiple effective components in Huangqi decoction against liver fibrosis.

Phytomedicine 2021 Apr 18;84:153513. Epub 2021 Feb 18.

Department of Pharmacology, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Shanghai Key Laboratory of Compound Chinese Medicines, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address:

Background: Huangqi decoction (HQD) has been used to treat chronic liver diseases since the 11th century, but the effective components in HQD against liver fibrosis have not been definitively clarified.

Purpose: To investigate and identify multiple effective components in HQD against liver fibrosis using a pharmacokinetics-based comprehensive strategy.

Methods: The absorbed representative components in HQD and their metabolites were detected in human plasma and urine using high-resolution mass spectrometry combined with a database-directed method, and then pharmacokinetics in multiple HQD components in human plasma was analyzed by ultra-performance liquid chromatography coupled with triple-quadruple mass spectrometry. Furthermore, the anti-fibrotic effect of potential effective HQD components was studied in LX-2 cells and that of a multi-component combination of HQD (MCHD) was verified in a mouse CCl-induced hepatic fibrosis model.

Results: Twenty-four prototype components in HQD and 17 metabolites were identified in humans, and the pharmacokinetic characteristics of 14 components were elucidated. Among these components, astragaloside IV, cycloastragenol, glycyrrhizic acid, glycyrrhetinic acid, liquiritigenin, and isoliquiritigenin downregulated the mRNA expression of α-SMA; cycloastragenol, calycosin-7-O-β-D-glucoside, formononetin, glycyrrhetinic acid, liquiritin, and isoliquiritin downregulated the mRNA expression of Col I; and calycosin, liquiritigenin, isoliquiritigenin, cycloastragenol, and glycyrrhetinic accelerated the apoptosis of LX-2 cells. MCHD reduced serum aminotransferase activity and hepatic collagen fibril deposition in mice with CCl-induced hepatic fibrosis.

Conclusion: Using the pharmacokinetics-based comprehensive strategy, we revealed that multiple effective HQD components act together against liver fibrosis.
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http://dx.doi.org/10.1016/j.phymed.2021.153513DOI Listing
April 2021

Evaluating the effects of temperature and time on heterocyclic aromatic amine profiles in roasted pork using combined UHPLC-MS/MS and multivariate analysis.

Food Res Int 2021 Mar 18;141:110134. Epub 2021 Jan 18.

Institute of Agro-products Processing, Anhui Academy of Agricultural Science, Hefei 230031, China. Electronic address:

In this study, ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) combined with principal component analysis (PCA) were used to investigate the effects of process conditions on the profiles of carcinogenic and mutagenic heterocyclic aromatic amine (HAA) in the pork roasted at 175 °C, 200 °C, 225 °C and 250 °C for 10, 15, 20, 25, 30, 35 and 40 min. Twelve HAAs from four categories, including carboline (Norharman, Harman, and Phe-p-1), imidazopyridine (PhIP, 4'-OH-PhIP, DMIP, and 1,5,6-TMIP), imidazoquinoline (IQ, IQ [4,5-b], and MeIQ), and imidazoquinoxaline (MeIQx and 4,8-DiMeIQx), were detected, quantified and used to compose the HAA profiles in roasted pork. After being Analyzed by PCA, the distributions of HAA profiles from different temperature on the PCA score plot demonstrated that there are significant differences among the HAA profiles from different temperatures. The loading plot also showed that PhIP, 4'-OH-PhIP, IQ[4,5-b], and MeIQ were mainly responsible for the difference. The profiles from higher temperature distribute more scattered than the lower ones, illustrating that the time effects on the HAA profiles from higher temperature are stronger than the lower ones. Comparing the score and loading plots of different heating times, the diversities of the HAA profiles at different temperatures increased under prolonged heating because of the changingpyridines levels. The results of PCA that comparing the HAA from different categories displayed that the formation features of four categories HAAs were significantly differed because of their formation discrepancy under low temperatures and short-term roasting. Using HAA profiles as an entirety, these findings obtained in this study are more close to the real process of HAA formation in roasted pork, and make the complex effects of temperature and time on multiple HAA formations more simply to be concluded.
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http://dx.doi.org/10.1016/j.foodres.2021.110134DOI Listing
March 2021

Progesterone Suppresses -Induced Inflammation Through Inhibition of NLRP3 Inflammasome Pathway in THP-1 Cells and Murine Models.

Front Microbiol 2021 9;12:570093. Epub 2021 Feb 9.

Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Asymptomatic/subclinical gonococcal infections in females continue to be prevalent within the general population, thus emerging as a global health problem. However, the reasons for these clinical manifestations are unknown. Our group had previously found out that in females, asymptomatic gonococcal infections correlate with higher serum progesterone (P4) levels and lower IL-1β levels in cervical secretions. We used murine infection model and THP-1 cells to determine whether P4 exerts anti-inflammatory effects on gonococcal infections. In the murine infection model, P4 (1 mg/day) inhibited the inflammatory effects induced by gonococcal infections which led to decreased neutrophil infiltration, reduced polymorphonuclear neutrophils (PMNs) numbers, IL-1β, TNF-α, and IL-6 levels in vaginal secretions. In addition, P4 down-regulated the mRNA and protein levels of NLRP3, associated with lower mRNA levels of pro-IL-1β, repressed caspase-1 activity in genital tissues and THP-1 cells. Moreover, P4 suppressed the phosphorylation levels of NF-κB and attenuated (, gonococci or GC)-induced ROS generation. This is consistent with the two signals required for activation of the NLRP3 (NOD-, LRR-, and pyrin domain-containing protein 3) inflammasome. In conclusion, our result shows that P4 suppresses the gonococci induced-inflammation, especially through the NLRP3 inflammasome pathway, and partially explains the pathogenesis of asymptomatic GC infection in women.
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http://dx.doi.org/10.3389/fmicb.2021.570093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900005PMC
February 2021

Inhibition of hsa_circ_0003489 shifts balance from autophagy to apoptosis and sensitizes multiple myeloma cells to Bortezomib via miR-874-3p/HDAC1 axis.

J Gene Med 2021 Feb 24:e3329. Epub 2021 Feb 24.

Department of Hematology, Longgang District People's Hospital of Shenzhen, Shenzhen, Guangdong Province, P.R. China.

Background: Circular RNAs (circRNAs) crucially regulate tumor progression. In this study, we examined the functional roles and mechanisms of hsa_circ_0003489 in multiple myeloma (MM).

Methods: Upon altering the expressions of hsa_circ_0003489, miR-874-3p, and/or histone deacetylase 1 (HDAC1) in MM1.R cells and treating them with Bortezomib (BTZ), cell viability was examined by CCK-8 assay, cell proliferation by Ki-67 immunofluorescence, apoptosis by TUNEL staining, flow cytometry and western blot, autophagy by electron microscopy and western blot. The interaction between hsa_circ_0003489 and miR-874-3p as well as that between miR-874-3p and HDAC1 were examined by expressional analysis, dual luciferase reporter assay, and RNA immunoprecipitation. The in vivo impacts of hsa_circ_0003489 on MM growth and sensitivity to BTZ were examined using an MM xenograft mouse model.

Results: Knocking down hsa_circ_0003489 significantly inhibited the viability, cell proliferation, and the autophagy, while promoted the apoptosis of MM cells in vitro and MM xenograft in vivo. Suppressing hsa_circ_0003489 also further boosted the cytotoxic effects of BTZ in MM cells and reversed its promoting effect on autophagy. Mechanically, hsa_circ_0003489 acted as a sponge of miR-874-3p and positively regulated the expression of miR-874-3p target, HDAC1. MiR-874-3p and HDAC1 essentially mediated the effects of hsa_circ_0003489 on cell viability, proliferation, apoptosis, and autophagy.

Conclusion: The hsa_circ_0003489/miR-874-3p/HDAC1 axis critically regulates the balance between apoptosis and autophagy. Silencing hsa_circ_0003489 sensitizes MM cells to BTZ by prohibiting autophagy and thus may boost the therapeutic effects of BTZ.
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http://dx.doi.org/10.1002/jgm.3329DOI Listing
February 2021

Prediction of marine mammal auditory-impact risk from Acoustic Deterrent Devices used in Scottish aquaculture.

Mar Pollut Bull 2021 Apr 20;165:112171. Epub 2021 Feb 20.

School of Mathematics & Maxwell Institute, University of Edinburgh, Edinburgh EH9 3FD, Scotland, UK. Electronic address:

Acoustic Deterrent Devices (ADDs) are used worldwide to deter pinnipeds from predating fish-aquaculture facilities. Desk-based noise-propagation modelling of six commercial ADD models, and a 'fictional' ADD was performed, the latter involving alternating source level, frequency, duty cycle, noise-exposure duration, and number of ADDs active simultaneously. Potential auditory impacts on marine mammals were explored using the Southall et al. (2019) criteria. Depending on operational characteristics, real ADDs were predicted to cause Temporary Threshold Shift (TTS) to Very High Frequency (VHF) cetaceans at ranges of 4-31 km, and a single fictional device operating at the highest outputs tested was predicted to cause TTS to VHF cetaceans at up to 32 km. Cumulative effects of 23 real fish-farm ADDs produced noise across large swathes of the Inner-Hebrides. The single variable causing greatest reduction in potential impact to marine mammals from fictional ADDs was SL.
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http://dx.doi.org/10.1016/j.marpolbul.2021.112171DOI Listing
April 2021

Nanozymes as Enzyme Inhibitors.

Int J Nanomedicine 2021 12;16:1143-1155. Epub 2021 Feb 12.

Institute of Translational Medicine, Department of Pharmacology, School of Medicine, Yangzhou University, Yangzhou, Jiangsu, 225001, People's Republic of China.

Nanozyme is a type of nanomaterial with intrinsic enzyme-like activity. Following the discovery of nanozymes in 2007, nanozyme technology has become an emerging field bridging nanotechnology and biology, attracting research from multi-disciplinary areas focused on the design and synthesis of catalytically active nanozymes. However, various types of enzymes can be mimicked by nanomaterials, and our current understanding of nanozymes as enzyme inhibitors is limited. Here, we provide a brief overview of the utility of nanozymes as inhibitors of enzymes, such as R-chymotrypsin (ChT), β-galactosidase (β-Gal), β-lactamase, and mitochondrial F0F1-ATPase, and the mechanisms underlying inhibitory activity. The advantages, challenges and future research directions of nanozymes as enzyme inhibitors for biomedical research are further discussed.
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http://dx.doi.org/10.2147/IJN.S294871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887156PMC
March 2021

Liuzijue is a promising exercise option for rehabilitating discharged COVID-19 patients.

Medicine (Baltimore) 2021 Feb;100(6):e24564

Department of Rehabilitation Medicine.

Background: Among discharged COVID-19 patients, the health-related quality of life is poor, and patients suffer from significant physical and psychological impairment. This study was designed to investigate the effects of Liuzijue exercise on the rehabilitation of COVID-19 patients.

Methods: Thirty three eligible patients with COVID-19 were enrolled in the study after discharge. All the participants practiced Liuzijue exercise once per day for 20 minutes over 4 weeks. Data were collected at baseline and the end of the intervention. Primary outcomes involved functional capacity and secondary outcomes involved quality of life.

Results: The maximal inspiratory pressure (MIP), peak inspiratory flow (PIF), and diaphragm movement in deep breathing (DM-DB) of patients increased significantly after 4 weeks of intervention. The dyspnea was also alleviated and exercise capacity was significantly improved. In terms of quality of life, physical functioning and role-physical scores were significantly increased. Moreover, Liuzijue could significantly alleviate the depression and anxiety status of the patients.

Conclusion: Liuzijue exercise is a viable alternative home exercise program that produced better functional capacity and quality of life in discharged patients with COVID-19. These findings also showed the necessity of rehabilitation intervention for cured COVID-19 patients.
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http://dx.doi.org/10.1097/MD.0000000000024564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886442PMC
February 2021

Aberrant promoter methylation of T-cadherin in sera is associated with a poor prognosis in oral squamous cell carcinoma.

Neoplasma 2021 Feb 11. Epub 2021 Feb 11.

Department of Clinical Laboratory, The Third Affiliated Hospital of Guangzhou Medical University, Liwan, Guangzhou, China.

T-cadherin functions as a suppressor gene, which is frequently inactivated by aberrant promoter methylation in several human cancers, but its methylation status in oral squamous cell carcinoma (OSCC) has been scarcely studied. Thus this study aimed at exploring the clinical significance and prognostic value of T-cadherin methylation in sera of patients with OSCC. Methylation-specific PCR (MSP) and bisulfate sequencing PCR (BSP) was performed to examine the methylation status of T-cadherin. Then, the associations between methylation status of T-cadherin and various clinicopathological variables or patient survival were investigated in 202 patients with OSCC and 68 controls. T-cadherin methylation was detected in 62 out of 202 (30.7%) patients with OSCC, and the methylation status of T-cadherin in corresponding tissues was confirmed by BSP. Methylation of T-cadherin was significantly associated with advanced tumor T-stage (P < 0.001) and N-stage (P = 0.003), positive lymphatic metastasis (P = 0.004) and tumor recurrence (P = 0.001). In addition, patients with methylation of T-cadherin had worse overall survival (P = 0.018) and progression-free survival (P < 0.001) than patients without, and methylation of T-cadherin in sera was an independent prognostic factor for worse overall survival (HR: 3.626, 95% CI: 1.112-9.624, P = 0.007) and progression-free survival (HR: 4.201, 95% CI: 1.562-10.038, P < 0.001) of patients with OSCC. These results demonstrated that methylation of T-cadherin was frequently detected in sera of patients with OSCC, which was associated with risk factors of poor outcomes, and may act as a potential independent prognostic marker for patients with OSCC.
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http://dx.doi.org/10.4149/neo_2021_201110N1203DOI Listing
February 2021

Construction of a synthetic microbial community for the biosynthesis of volatile sulfur compound by multi-module division of labor.

Food Chem 2021 Jun 12;347:129036. Epub 2021 Jan 12.

Key Laboratory of Industrial Biotechnology of Ministry of Education, State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address:

3-(Methylthio)-1-propanol, reminiscent of cauliflower and cooked vegetable aroma, is an important sulfur compound in Baijiu. It is important to develop a method to increase 3-(methylthio)-1-propanol content to improve flavor quality of products. In this study, a synthetic microbial community was employed to enhance the content of 3-(methylthio)-1-propanol by multi-module division of labor approach. Firstly, the synthetic pathway of 3-(methylthio)-1-propanol was reconstructed and classified into three modules. Later, the hyper producers in each module were isolated and negative interaction between the members was relieved. Finally, a synthetic microbial community was constructed using three species containing one hyper producer from each module. Furthermore, the transcription characteristics of the species in each module were validated by metatranscriptomic analysis. The constructed synthetic microbial community can be used to biosynthesize 3-(methylthio)-1-propanol for Baijiu. This work provided a novel and workable strategy to design synthetic microbial community to enhance the flavor feature of other fermented foods.
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http://dx.doi.org/10.1016/j.foodchem.2021.129036DOI Listing
June 2021

Dll4/Notch1 signalling pathway is required in collective invasion of salivary adenoid cystic carcinoma.

Oncol Rep 2021 Mar 18;45(3):1011-1022. Epub 2021 Jan 18.

State Key Laboratory of Oral Diseases, Department of Oral Pathology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

High expression of δ‑like ligand 4 (Dll4) is reportedly related to the invasion, metastasis, and clinical prognosis of various malignant tumours. Our previous study revealed that collective cell invasion was a common pattern in salivary adenoid cystic carcinoma (SACC). However, the roles of the Dll4/Notch1 signalling pathway in the collective invasion of SACC remain unclear. The present study revealed that Dll4 expression was higher at the invasive front of SACC, and that this upregulation was associated with solid tumour type, high TNM grade, and high rates of metastasis and recurrence. Furthermore, the expression levels of Notch1 and Dll4 were positively correlated at the invasive front, and a three‑dimensional (3D) culture model revealed that leader cells showed high expression of Dll4, while follower cells showed high expression of Notch1. Moreover, silencing of Dll4 expression using small interfering RNA reduced the migration, invasion, and collective invasion of SACC cells, and these abilities were rescued by Notch1 overexpression. Finally, SACC collective invasion was increased via the Dll4/Notch1 signalling pathway in experiments that involved a stiff 3D gel, hypoxia and co‑culture with human endothelial cells. These findings indicated that the Dll4/Notch1 signalling pathway may be involved in the collective invasion of SACC, which may help to provide possible targets for the treatment of SACC.
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http://dx.doi.org/10.3892/or.2021.7939DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859997PMC
March 2021

[Nutritional analysis of eight kinds of prefabricated beef steaks].

Wei Sheng Yan Jiu 2020 Nov;49(6):969-977

Center for Disease Control and Prevention of Anhui Province, Hefei 230601, China.

Objective: A investigate on the nutritional quality of the prefabricated beef steak in the market was conducted, aiming to provide scientific basis for consumers in the consumption of prefabricated beef steaks.

Methods: The basic nutriments, amino acids, fatty acids, vitamins and mineral elements of 8 kinds of prefabricated beef steaks were analyzed and evaluated.

Results: It was showed that the contents of crude ash(1. 3-2. 4 g/100 g edible) and crude fat(1. 3-10. 0 g/100 g edible) in the 8 kinds of prefabricated beef steaks were higher than those of raw beef, while the contents of protein(11. 4-17. 2 g/100 g edible) was lower than those of raw beef. All the 8 kinds of prefabricated beef steaks had 18 kinds of amino acids and the content of histidine in children's steak(0. 57 g/100 g edible) was the highest. With the exception of snowflake steak and bulk steak, the first limiting amino acid of other 6 kinds of prefabricated beef steaks was valine. The essential amino acid composition of the 8 kinds of prefabricated steaks was close to that of FAO/WHO standard protein. This meant they were all high quality protein sources. The proportion of saturated fatty acids(48. 0%-62. 0%) was the highest, followed by monounsaturated fatty acids(32. 2%-46. 6%) and polyunsaturated fatty acids(0. 7%-7. 6%). All the 8 kinds of prepared beef steak didn't meet the S∶M∶P standard recommended by American Heart Association. There were differences in the content of vitamins among 8 kinds of prefabricated beef steaks. In terms of mineral elements, the content of Na(344-689 mg/100 g edible), Ca(6-15 mg/100 g edible), Fe(1. 0-1. 9 mg/100 g edible), Cu(0. 029-0. 050 mg/100 g edible), Mn(0. 056-0. 183 mg/100 g edible) in prefabricated steak was higher than that in raw beef.

Conclusion: There were interspecific differences in the nutritional element content and quality among different kinds of prefabricated steaks. Compared with the raw beef, the fat content was higher and protein content was lower, the composition of fatty acids was less reasonable than fish and pork.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2020.06.017DOI Listing
November 2020

Proximate underwater soundscape of a North Sea offshore petroleum exploration jack-up drilling rig in the Dogger Bank.

J Acoust Soc Am 2020 12;148(6):3971

School of Mathematics and Maxwell Institute, University of Edinburgh, Edinburgh, Scotland EH9 3FD, United Kingdom.

Little is known about localized, near-field soundscapes during offshore hydrocarbon drilling campaigns. In the Dogger Bank, North Sea, underwater noise recordings were made 41-60 m from the drill stem of the Noble Kolskaya jack-up exploration drilling rig. The aims were to document noise received levels (RLs) and frequency characteristics of rig-associated near-field noise. The rig produced sound pressure levels (SPLs) of 120 dB re 1 μPa in the frequency range of 2-1400 Hz. Over transient periods, RLs varied by 15-20 dB between softest (holding) and noisiest (drilling) operations. Tonal components at different frequencies varied with depth. Support vessel noise was significantly louder than the jack-up rig at frequencies <1 kHz, even in its noisiest "boulder-drilling" phase, though radiated noise levels were higher above 2 kHz. Rig SPLs fell rapidly above 8 kHz. Marine mammals, such as harbor porpoise (Phocoena phocoena) forage regularly near offshore oil and gas rigs and platforms, and it is predicted that animals experience different noise regimes while traversing the water column and can potentially detect the higher-frequency components of drilling noise to a distance of 70 m from the source; however, while levels were unlikely to cause auditory injury, effects on echolocation behavior are still unknown.
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http://dx.doi.org/10.1121/10.0002958DOI Listing
December 2020

Neurocognitive improvement after angioplasty in patients with chronic middle cerebral artery stenosis and cerebral ischemia.

Ann Palliat Med 2021 Feb 11;10(2):1642-1649. Epub 2020 Dec 11.

Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, China.

Background: The use of middle cerebral artery (MCA) angioplasty compared with drug therapy has been controversial. Few studies have reported the correlations between cognitive function improvement and MCA angioplasty. This study aimed to explore neurocognitive function after angioplasty in patients with middle cerebral artery stenosis (MCAS) and objective cerebral ischemia.

Methods: We identified 14 patients diagnosed with MCAS aged 45-65 years. Neurocognitive function evaluation was performed by 2 independent clinical psychologists using the Mini-Mental State Examination (MMSE), Montreal cognitive assessment scale (MoCA), and Multi-Dimensional Psychology. All patients received general anesthesia, underwent diagnostic cerebral angiography (DSA) via the femoral route and angioplasty, and then were sent to the neurologic intensive care unit (NICU) for overnight hemodynamic and neurologic monitoring. Aspirin and clopidogrel treatments were continued for 3 months after successful intervention. Complete neurologic examinations, including assessment with the National Institutes of Health Stroke (NIHSS), and modulate RANK score (MRS) were conducted by 2 independent neurologists. The patients received a family follow-up at 1 week, 1 month, and 3 months after the interventional procedure. Neurologic sequelae, intracranial hemorrhages, and deaths were recorded as an endpoint. Follow-up clinical and imageological examinations were scheduled at 6 months after the intervention. Follow-up brain computed tomography (CT) perfusion or magnetic resonance angiography (MRA) perfusion scans performed by 2 imageological scanners were scheduled 6 months after the procedure.

Results: Angioplasty technical success was achieved in 14 parents (100%). We found that 10 patients did not have recurrent MCAS in the angioplasty site and had significant improvements in the associated brain perfusion situation and cognitive founction as compared before and after angioplasty. Also, 4 patients had evident restenosis in the angioplasty site. In the nonstenosis group, we found significant improvements in the MMSE, 3-dimensional (3D) mental rotation, simple calculation, and spatial working memory. In the recurrent stenosis group, we found no statistically significant changes in cognitive function compared with the baseline and after a 6-month follow up. There were high correlations between the changes in perfusion and the changes in word and picture memory. There was a significant correlation between the change in perfusion with MMSE (-0.522), spatial working memory (0.655), and Raven's progressive matrices test (0.637); a moderate correlation with 3D rotation (0.413), and simple calculation (-0.359); and weak correlation with visual tracking (0.026) and MoCA (0.279).

Conclusions: Angioplasty surgery significantly improves neurocognitive function in patients with middle cerebral artery stenosis (MCAS) and objective cerebral ischemia.
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http://dx.doi.org/10.21037/apm-20-15DOI Listing
February 2021

Vietnamella chebalingensis, a new species of the family Vietnamellidae (Ephemeroptera) from China based on morphological and molecular data.

Zootaxa 2020 Oct 27;4868(2):zootaxa.4868.2.2. Epub 2020 Oct 27.

Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, Guangdong, China.

Vietnamella chebalingensis Tong, sp. nov. is described based on larval stage from China. The new species is distinguished from its congeners by the following combination of characters: body purple black with mottled white markings; length of inner pairs of cephalic projection approximately half of the outer pairs; posterior margin of abdominal tergites I-VI and VIII-X each with a pair of pointed projections, but tergite VII bearing only a single projection. The morphological evidence and molecular data (COI, Kimura 2-parameter) support that this new species represents the sixth species of the genus Vietnamella. An identification key to known larvae of the genus Vietnamella is provided.
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http://dx.doi.org/10.11646/zootaxa.4868.2.2DOI Listing
October 2020