Publications by authors named "Jian Huang"

2,153 Publications

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Uplift, climate and biotic changes at the Eocene-Oligocene transition in south-eastern Tibet.

Natl Sci Rev 2019 May 12;6(3):495-504. Epub 2018 Jun 12.

Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China.

The uplift history of south-eastern Tibet is crucial to understanding processes driving the tectonic evolution of the Tibetan Plateau and surrounding areas. Underpinning existing palaeoaltimetric studies has been regional mapping based in large part on biostratigraphy that assumes a Neogene modernization of the highly diverse, but threatened, Asian biota. Here, with new radiometric dating and newly collected plant-fossil archives, we quantify the surface height of part of the south-eastern margin of Tibet in the latest Eocene (∼34 Ma) to be ∼3 km and rising, possibly attaining its present elevation (3.9 km) in the early Oligocene. We also find that the Eocene-Oligocene transition in south-eastern Tibet witnessed leaf-size diminution and a floral composition change from sub-tropical/warm temperate to cool temperate, likely reflective of both uplift and secular climate change, and that, by the latest Eocene, floral modernization on Tibet had already taken place, implying modernization was deeply rooted in the Palaeogene.
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http://dx.doi.org/10.1093/nsr/nwy062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291530PMC
May 2019

Effects of chitooligosaccharide-zinc on the ovarian function of mice with premature ovarian failure via the SESN2/NRF2 signaling pathway.

Chin J Nat Med 2021 Oct;19(10):721-731

Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518000, China; Key Laboratory of Reproductive Physiology and Pathology of Jiangxi Province, Nanchang University, Nanchang 330006, China. Electronic address:

Chitooligosaccharide-zinc (COS·Zn) is a powerful anti-oxidant and anti-aging scavenger, whose anti-oxidative ability immensely exceeds vitamin C. Therefore, this study was aimed to investigate the protective effects of COS·Zn against premature ovarian failure (POF) and potential mechanisms. Female KM adult mice were divided into the following groups: a treatment group (150 mg·kg·d COS·Zn), a treatment group (300 mg·kg·d COS·Zn), a prevention group, two control groups and two CY/BUS groups. COS·Zn (150, 300 mg·kg·d) and COS·Zn (300 mg·kg·d) were therapeutically and preventatively administered to POF mice in the treatment and prevention studies, respectively. All the groups were administered for 21 days. Fewer primary and secondary follicles were observed in the COS·Zn-treated groups (including the treatment and prevention groups) than those of the control groups. Meanwhile, the ovarian index and the levels of FSH and LH notably increased in the treatment and prevention groups compared with those in the CY/BUS group. The levels of MVH, OCT4 and PCNA in the treatment group (300·kg·d COS·Zn) and MVH in the prevention group remarkably increased compared with those in the CY/BUS groups. Meanwhile, the levels of P53 and P16 protein were down-regulated in the treatment and prevention groups compared with those in the CY/BUS groups. Additionally, the amounts of Sestrin2 (SESN2) and SOD2 protein were obviously higher in the treatment group (150 mg·kg·d COS·Zn) than those in the CY/BUS groups. Similarly, the amounts of NRF2 and SESN2 protein were up-regulated in the prevention group. Besides, an increased GSH level was observed in the two treatment groups, compared with that in the CY/BUS groups, and the same trend was also present in the prevention group. Taken together, COS·Zn improves the ovarian and follicular development through regulating the SESN2/NRF2 signaling pathway. These results suggest the role of COS·Zn as a novel agent for the treatment and prevention of POF.
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http://dx.doi.org/10.1016/S1875-5364(21)60084-5DOI Listing
October 2021

Atorvastatin Ester Regulates Lipid Metabolism in Hyperlipidemia Rats via the PPAR-signaling Pathway and HMGCR Expression in the Liver.

Int J Mol Sci 2021 Oct 14;22(20). Epub 2021 Oct 14.

Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang 110016, China.

Atorvastatin ester (Ate) is a structural trim of atorvastatin that can regulate hyperlipidemia. The purpose of this study was to evaluate the lipid-lowering effect of Ate. Male Sprague Dawley (SD) rats were fed a high-fat diet for seven months and used as a hyperlipidemia model. The lipid level and liver function of the hyperlipidemia rats were studied by the levels of TG, TC, LDL, HDL, ALT, and AST in serum after intragastric administration with different doses of Ate. HE staining was used to observe the pathological changes of the rat liver and gastrocnemius muscle. The lipid deposits in the liver of rats were observed by staining with ORO. The genes in the rat liver were sequenced by RNA-sequencing. The results of the RNA-sequencing were further examined by qRT-PCR and western blotting. Biochemical test results indicated that Ate could obviously improve the metabolic disorder and reduce both the ALT and AST levels in serum of the hyperlipidemia rats. Pathological results showed that Ate could improve HFD-induced lipid deposition and had no muscle toxicity. The RNA-sequencing results suggested that Ate affected liver lipid metabolism and cholesterol, metabolism in the hyperlipidemia-model rats may vary via the PPAR-signaling pathway. The western blotting and qRT-PCR results demonstrated the Ate-regulated lipid metabolism in the hyperlipidemia model through the PPAR-signaling pathway and HMGCR expression. In brief, Ate can significantly regulate the blood lipid level of the model rats, which may be achieved by regulating the PPAR-signaling pathway and HMGCR gene expression.
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http://dx.doi.org/10.3390/ijms222011107DOI Listing
October 2021

Apoptosis-induced translocation of centromere protein F in its corresponding autoantibody production in hepatocellular carcinoma.

Oncoimmunology 2021 18;10(1):1992104. Epub 2021 Oct 18.

National Clinical Research Center for Digestive Disease, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Serum autoantibodies against tumor-associated antigen have important value in the early diagnosis of hepatocellular carcinoma (HCC), but the mechanism of autoantibody production is poorly understood. We previously showed that autoantibodies against the centromere protein F (CENPF) may be useful as an early diagnostic marker for HCC. Here we explored the mechanism of cell apoptosis-based CENPF autoantibody production and verified the correlation of CENPF autoantibody level with HCC development. We demonstrated that CENPF was overexpressed and aberrantly localized throughout the nuclei and cytoplasm in human HCC cells compared with hepatic cells. CENPF overexpression promoted the production of CENPF autoantibodies in a manner that correlated with tumor growth of mouse HCC model. During apoptosis of HCC cells, CENPF protein translocated to apoptotic vesicles and relocalized at the cell surface. Through isolating apoptotic components, we found apoptotic body and blebs with lower CD31 and CD47 expression more effectively induced DC phagocytosis and maturation compared with apoptotic intact cells in vitro, and this DC response was independent of CENPF expression. Moreover, injection of mice with apoptotic bodies and blebs effectively induced an immune response and the production of CENPF-specific antibodies. Our findings provide a first elucidation of mechanisms underlying the CENPF autoantibody production via cell apoptosis-induced CENPF translocation, and demonstrate a direct correlation between CENPF autoantibody levels and HCC progression, suggesting the potential of CENPF autoantibody as an HCC diagnostic marker.
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http://dx.doi.org/10.1080/2162402X.2021.1992104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8525945PMC
October 2021

Efficacy of left ventricular unloading strategies during venoarterial extracorporeal membrane oxygenation in patients with cardiogenic shock: a protocol for a systematic review and Bayesian network meta-analysis.

BMJ Open 2021 Oct 19;11(10):e047046. Epub 2021 Oct 19.

Department of Cardiac Surgery, Lanzhou University Second Hospital, Lanzhou, Gansu, China

Introduction: Venoarterial extracorporeal membrane oxygenation (VA-ECMO) has been widely used for patients with refractory cardiogenic shock. A common side effect of this technic is the resultant increase in left ventricular (LV) afterload which could potentially aggravate myocardial ischaemia, delay ventricular recovery and increase the risk of pulmonary congestion. Several LV unloading strategies have been proposed and implemented to mitigate these complications. However, it is still indistinct that which one is the best choice for clinical application. This Bayesian network meta-analysis (NMA) aims to compare the efficacy of different LV unloading strategies during VA-ECMO.

Methods And Analysis: PubMed, Embase, the Cochrane Library and the International Clinical Trials Registry Platform will be explored from their inception to 31 December 2020. Random controlled trials and cohort studies that compared different LV unloading strategies during VA-ECMO will be included in this study. The primary outcome will be in-hospital mortality. The secondary outcomes will include neurological complications, haemolysis, bleeding, limb ischaemia, renal failure, gastrointestinal complications, sepsis, duration of mechanical ventilation, length of intensive care unit and hospital stays. Pairwise and NMA will respectively be conducted using Stata (V.16, StataCorp) and Aggregate Data Drug Information System (V.1.16.5), and the cumulative probability will be used to rank the included LV unloading strategies. The risk of bias will be conducted using the Cochrane Collaboration's tool or Newcastle-Ottawa Quality Assessment Scale according to their study design. Subgroup analysis, sensitivity analysis and publication bias assessment will be performed. The Grading of Recommendations Assessment, Development and Evaluation will be conducted to explore the quality of evidence.

Ethics And Dissemination: Either ethics approval or patient consent is not necessary, because this study will be based on literature. The results will be disseminated through peer-reviewed publications and conference presentations.

Prospero Registration Number: CRD42020165093.
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http://dx.doi.org/10.1136/bmjopen-2020-047046DOI Listing
October 2021

Soluble B7-H5 Is a Novel Diagnostic, Severity, and Prognosis Marker in Acute Pancreatitis.

Biomed Res Int 2021 8;2021:1223850. Epub 2021 Oct 8.

Department of Emergency, Internal Medicine of the First Affiliated Hospital, Suzhou University, Suzhou, 215000 Jiangsu, China.

As an important ligand in T lymphocyte costimulatory pathways, B7-H5 is involved deeply in the immune response in various diseases. However, its clinical usefulness as an early indicator in acute pancreatitis (AP) remains unclear. In this study, the levels of sB7-H5 and cytokines in plasma samples of 75 AP patients, 20 abdominal pain patients without AP, and 20 healthy volunteers were determined. Then, the correlation of sB7-H5 and clinical features, cytokines, the Ranson score, APACHE II score, Marshall score, and BISAP score was analysed, and the value of sB7-H5 for diagnostic, severity, and prognosis of AP was evaluated. We found that the levels of sB7-H5 were specifically upregulated in AP patients. Receiver operating characteristic (ROC) analysis revealed that sB7-H5 can identify AP patients from healthy or abdominal pain patients with 78.9% or 86.4% sensitivity and 93.3% or 90.0% specificity. Further analysis showed that the levels of sB7-H5 were significantly correlated with WBC ( = 0.004), GLU ( = 0.008), LDH ( < 0.001), Ca ( = 0.006), AST ( = 0.009), PLT ( = 0.041), IL-6 ( < 0.001), IL-10 ( < 0.001), and TNF- ( < 0.001). And levels of sB7-H5 were gradually increased among patients with mildly acute pancreatitis (MAP), moderately severe acute pancreatitis (MSAP), and severe acute pancreatitis (SAP). It can distinguish the severity of AP with good sensitivity and specificity. Moreover, when dividing the patients into two groups according to the median level of sB7-H5, the local complication and length of stay of low levels of the sB7-H5 group were significantly less than those in high levels of the sB7-H5 group. And the levels of sB7-H5 in AP patients were significantly correlated with the Ranson score ( < 0.001), APACHE II score ( < 0.001), Marshall score ( < 0.001), and BISAP score ( < 0.001). The AUCs of assessing local complications of sB7-H5 at day 1 and day 3 were 0.704 ( = 0.0024) and 0.727 ( = 0.0373). These results showed the potential value of sB7-H5 as a diagnostic, severity, and prognosis marker of AP.
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http://dx.doi.org/10.1155/2021/1223850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8519671PMC
October 2021

Prevalence of Complementary Feeding Indicators and Associated Factors Among 6- to 23-Month Breastfed Infants and Young Children in Poor Rural Areas of China.

Front Public Health 2021 1;9:691894. Epub 2021 Oct 1.

Chinese Center for Disease Control and Prevention, National Institute for Nutrition and Health, Beijing, China.

This study aimed to estimate the status of complementary feeding (CF) and its associated factors among 6-23-month breastfed infants and young children (IYC). We used secondary data from the China Nutrition Improvement Project on Children in Poor Areas in 2018. The status of CF was provided by parents of IYC through 24-h dietary recall. The study included 13,972 6-23-month-old breastfed IYC comprising 24.7% 6-8-month, 28.5% 9-11-month, 31.4% 12-17-month, and 18-23-month IYC. The highest percentage of IYC introduced to cereal foods was 84.8%. Nearly, 83.6% of 6-8-month infants were introduced to solid or semi-solid food. The prevalence of meeting requirements of non-dairy animal source food and minimum acceptable diet (MAD) was 75.3 and 35.1% of 6-23-month IYC, respectively, and was significantly higher in older than younger IYC ( < 0.001). Age of IYC, education level of parents, paternal employment, and nutrition knowledge of parents were positively associated factors for the prevalence of meeting requirements of MAD, and diarrhea at 2 weeks and maternal employment were negatively associated with MAD. Totally, the prevalence of meeting the requirements of MAD was relatively lower in breastfed IYC. The government should scale up appropriate CF with consideration of food availability.
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http://dx.doi.org/10.3389/fpubh.2021.691894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8517442PMC
October 2021

The clinical significance of vitamin D levels and vitamin D receptor mRNA expression in colorectal neoplasms.

J Clin Lab Anal 2021 Oct 15:e23988. Epub 2021 Oct 15.

Department of Gastroenterology, Laboratory of Digestive Diseases, Ningbo First Hospital, Ningbo, China.

Background/aim: This study aimed to investigate the clinical significance of changes in vitamin D [25(OH)D] levels and vitamin D receptor (VDR) mRNA expression in colorectal adenoma development.

Methods: Plasma concentrations of 25(OH)D and mRNA expression of VDR in tissues were determined by enzyme-linked immunosorbent assay (ELISA) and real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), respectively. In addition, the concentration of plasma 25(OH)D and levels of VDR mRNA in tissues were compared among healthy individuals and adenoma and adenocarcinoma patients.

Results: Vitamin D receptor expression in colorectal adenocarcinoma tissues was significantly lower than that in para-cancerous tissues that were >5 cm away from malignant tumor sites (p < 0.01). The level of VDR expression in normal colorectal tissues from healthy individuals was significantly higher than that in colorectal adenomas (p < 0.01) and colorectal adenocarcinomas (p < 0.01); however, the VDR expression was not significantly different between colorectal adenomas and colorectal adenocarcinomas (p = 0.106). The concentration of 25(OH)D in healthy individuals was significantly higher than that in patients with colorectal adenomas (p < 0.01) and colorectal adenocarcinomas (p < 0.01); however, the concentration of 25(OH)D was not significantly different between colorectal adenomas and colorectal adenocarcinomas (p = 0.489). A low concentration of 25(OH)D was considered a risk factor for colorectal adenoma and colorectal adenocarcinoma, with odds ratios of 4.875 and 2.925, respectively.

Conclusions: The 25(OH)D levels and VDR mRNA expression might be associated with the development of colorectal adenoma and its progression to adenocarcinoma.
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http://dx.doi.org/10.1002/jcla.23988DOI Listing
October 2021

The influence of different THA surgical approaches on Patient's early postoperative anxiety and depression.

BMC Musculoskelet Disord 2021 Oct 8;22(1):858. Epub 2021 Oct 8.

State Key Laboratory of Pharmaceutical Biotechnology, Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, 321 Zhongshan Road, Nanjing, 210008, Jiangsu, PR China.

Introduction: Total hip arthroplasty (THA) is generally considered to be one of the most successful orthopedic surgical procedures. However, no research has been conducted on the postoperative mental health of patients who underwent different approaches of THA. This paper seeks to compare the differences among three THA approaches: the normal lateral approach (NLA), the direct anterior approach (DAA) and the orthopädische chirurgie münchen (OCM) regarding their influence on patients' postoperative anxiety and depression.

Method: A total of 95 THA patients were recruited for this study. All patients' preoperative information including results of Harris, SF-36 and Visual Analogue Scale (VAS) was carefully evaluated. Surgery-related data as well as five-day postoperative data were also collected. Three months after the surgery, a telephone follow-up was conducted to further evaluate patients' HADS and SF-36 results.

Result: In the three-month postoperative evaluation of anxiety and depression, the NLA group scored significantly higher than both the DAA group and the OCM group, which was found relevant to the patient's incision length and five-day postoperative VAS results. A correlation between anxiety scores and the days of postoperative hospitalization was also noticed. Further analysis of patients' psychological state based on the SF-36 results revealed considerable differences in viability (VT) and social function (SF) between the NLA group and the OCM group. Other surgery-related data and postoperative data all demonstrated better results of the DAA group and the OCM group compared to the NLA group.

Conclusion: Among the three different surgical approaches of THA, DAA and OCM compared with NLA are found to ease patients' postoperative anxiety and depression.

Level Of Evidence: III.
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http://dx.doi.org/10.1186/s12891-021-04746-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8501680PMC
October 2021

α-Keto Acids as Triggers and Partners for the Synthesis of Quinazolinones, Quinoxalinones, Benzooxazinones, and Benzothiazoles in Water.

J Org Chem 2021 Oct 8. Epub 2021 Oct 8.

Key Laboratory for Green Chemistry of Jiangxi Province, Key Laboratory of Functional Small Molecules for Ministry of Education, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang 330022, China.

A general and efficient method for the synthesis of quinazolinones, quinoxalinones, benzooxazinones, and benzothiazoles from the reactions of α-keto acids with 2-aminobenzamides, benzene-1,2-diamines, 2-aminophenols, and 2-aminobenzenethiols, respectively, is described. The reactions were conducted under catalyst-free conditions, using water as the sole solvent with no additive required, and successfully applied to the synthesis of sildenafil. More importantly, these reactions can be conducted on a mass scale, and the products can be easily purified through filtration and washing with ethanol (or crystallized).
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http://dx.doi.org/10.1021/acs.joc.1c01497DOI Listing
October 2021

Xianling Gubao Capsule Prevents Cadmium-Induced Kidney Injury.

Biomed Res Int 2021 28;2021:3931750. Epub 2021 Sep 28.

Department of Pharmaceutical Science, Changchun University of Chinese Medicine, Changchun, China.

Xianling Gubao Capsule (XGC), a kind of capsule preparation of Chinese herbal officially approved for sale by the National Medical Products Administration (NMPA), has the effect of tonifying kidney and strengthening bones. Although the impact of XGC in treating bone diseases has been widely studied, the effect of XGC in kidney injury is unknown yet. The kidney injury model is established by intraperitoneal injection with cadmium chloride (CdCl). Before model establishment, each XGC group was pregavaged with XGC for 10 d. After 10 d, CdCl2 was injected intraperitoneally into the model group and each XGC group, each XGC group continued to be gavaged with XGC for 4 weeks, and the control group was gavaged with equal doses of distilled water once daily. The level of serum urea nitrogen (BUN) and serum creatinine (Cr) is evaluated by kit. The effect of XGC on protecting kidney injury in mice with kidney injury is analyzed by histopathology (HE stain), immunohistochemistry (IHC), and real-time fluorescence quantitative PCR (RT-qPCR). The results show that CdCl significantly increases the level BUN and Cr in serum and results in remarkable pathological changes in the nephron, including tubule edema, congestion, and necrosis. While oral administration of XGC can significantly decrease BUN and Cr in serum and prevent and protect the kidney from the above injuries. In addition, the protein expression of p-mTOR was remarkably reduced, and the ratio of LC3II/LC3I protein and mRNA was significantly increased in mice with oral administration of XGC. Our findings suggest that XGC can prevent and protect kidney injury by improving the state of renal tubular hyperemia and necrosis and reduce the level of BUN and Cr in cadmium poisoning mice.
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http://dx.doi.org/10.1155/2021/3931750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492235PMC
September 2021

Integrated Analysis of Microbiome and Transcriptome Data Reveals the Interplay Between Commensal Bacteria and Fibrin Degradation in Endometrial Cancer.

Front Cell Infect Microbiol 2021 21;11:748558. Epub 2021 Sep 21.

Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, China.

The gut-uterus axis plays a pivotal role in the pathogenesis of endometrial cancer (EC). However, the correlations between the endometrial microbiome and endometrial tumor transcriptome in patients with EC and the impact of the endometrial microbiota on hematological indicators have not been thoroughly clarified. In this prospective study, endometrial tissue samples collected from EC patients (n = 30) and healthy volunteers (n = 10) were subjected to 16S rRNA sequencing of the microbiome. The 30 paired tumor and adjacent nontumor endometrial tissues from the EC group were subjected to RNAseq. We found that and were enriched in the EC group with a high tumor burden. By integrating the microbiome and hematological indicators, a correlation was observed between and elevated serum D-dimer (DD) and fibrin degradation products (FDPs). Further transcriptome analysis identified 8 robust associations between and fibrin degradation-related genes expressed within ECs. Finally, the microbial marker of along with DD and FDPs showed a high potential to predict the onset of EC (AUC = 0.86). Our results suggest that the increasing abundance of in endometrial tissue combined with high serum DD and FDP contents may be important factors associated with tumor burden. The microbe-associated transcripts of host tumors can partly explain how promotes DD and FDPs.
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http://dx.doi.org/10.3389/fcimb.2021.748558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8490766PMC
October 2021

Kidney damage causally affects the brain cortical structure: A Mendelian randomization study.

EBioMedicine 2021 Oct 4;72:103592. Epub 2021 Oct 4.

Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China; Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, PR China. Electronic address:

Background: Alterations in the brain cortical structures of patients with chronic kidney disease (CKD) have been reported; however, the cause has not been determined yet. Herein, we used Mendelian randomization (MR) to reveal the causal effect of kidney damage on brain cortical structure.

Methods: Genome-wide association studies summary data of estimated glomerular filtration rate (eGFR) in 480,698 participants from the CKDGen Consortium were used to identify genetically predicted eGFR. Data from 567,460 individuals from the CKDGen Consortium were used to assess genetically determined CKD; 302,687 participants from the UK Biobank were used to evaluate genetically predicted albuminuria. Further, data from 51,665 patients from the ENIGMA Consortium were used to assess the relationship between genetic predisposition and reduced eGFR, CKD, and progressive albuminuria with alterations in cortical thickness (TH) or surficial area (SA) of the brain. Magnetic resonance imaging was used to measure the SA and TH globally and in 34 functional regions. Inverse-variance weighted was used as the primary estimate whereas MR Pleiotropy RESidual Sum and Outlier, MR-Egger and weighted median were used to detect heterogeneity and pleiotropy.

Findings: At the global level, albuminuria decreased TH (β = -0.07 mm, 95% CI: -0.12 mm to -0.02 mm, P = 0.004); at the functional level, albuminuria reduced TH of pars opercularis gyrus without global weighted (β = -0.11 mm, 95% CI: -0.16 mm to -0.07 mm, P = 3.74×10). No pleiotropy was detected.

Interpretation: Kidney damage causally influences the cortex structure which suggests the existence of a kidney-brain axis.

Funding: This study was supported by the Science and Technology Planning Project of Guangdong Province (Grant No. 2020A1515111119 and 2017B020227007), the National Key Research and Development Program of China (Grant No. 2018YFA0902803), the National Natural Science Foundation of China (Grant No. 81825016, 81961128027, 81772719, 81772728), the Key Areas Research and Development Program of Guangdong (Grant No. 2018B010109006), Guangdong Special Support Program (2017TX04R246), Grant KLB09001 from the Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes, and Grants from the Guangdong Science and Technology Department (2020B1212060018).
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http://dx.doi.org/10.1016/j.ebiom.2021.103592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498227PMC
October 2021

Carbon dots up-regulate heme oxygenase-1 expression towards acute lung injury therapy.

J Mater Chem B 2021 Oct 7. Epub 2021 Oct 7.

Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, 199 Ren'ai Road, Suzhou, 215123, Jiangsu, China.

Pneumonia is a kind of inflammation, which can cause high morbidity and mortality, and the treatment of pneumonia has received widespread attention. Heme oxygenase-1 (HMOX1) is a cell protective enzyme and can generate an anti-inflammatory response. Here, we demonstrate that degradable carbon dots (from L-ascorbic acid, CDs-1) can up-regulate the expression of HMOX1 in animal cells and tissues, which has a therapeutic effect on LPS-induced acute lung injury in mice. It was confirmed from experiments that CDs-1 could significantly up-regulate the expression of mRNA and the protein of HMOX1, which can increase the expression of HMOX1 by 5 times in a short time, decreasing the reactive oxygen species level in a cellular inflammation model induced by LPS. Furthermore, a series of comparative experiments show that CDs-1 could effectively treat acute lung injury and improve the survival rate of mice to 80%. Our work provides a practical way for the treatment of acute inflammation and the promising application of CDs in anti-inflammation.
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http://dx.doi.org/10.1039/d1tb01283eDOI Listing
October 2021

Exosomal hsa-miR-21-5p is a biomarker for breast cancer diagnosis.

PeerJ 2021 17;9:e12147. Epub 2021 Sep 17.

Department of Clinical Laboratory, Affiliated Hospital of Guizhou Medical UniversityGuiyang, Guizhou Province, China.

Purpose: Breast cancer (BC) is characterized by concealed onset, delayed diagnosis, and high fatality rates making it particularly dangerous to patients' health. The purpose of this study was to use comprehensive bioinformatics analysis and experimental verification to find a new biomarker for BC diagnosis.

Methods: We comprehensively analyzed microRNA (miRNA) and mRNA expression profiles from the Gene Expression Omnibus (GEO) and screened out differentially-expressed (DE) miRNAs and mRNAs. We used the miRNet website to predict potential DE-miRNA target genes. Using the Database for Annotation, Visualization and Integrated Discovery (DAVID), we performed Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses on overlapping potential target genes and DE-mRNAs. The protein-protein interaction (PPI) network was then established. The miRNA-mRNA regulatory network was constructed using Cytoscape and the analysis results were visualized. We verified the expression of the most up-regulated DE-miRNA using reverse transcription and a quantitative polymerase chain reaction in BC tissue. The diagnostic value of the most up-regulated DE-miRNA was further explored across three levels: plasma-derived exosomes, cells, and cell exosomes.

Results: Our comprehensive bioinformatics analysis and experimental results showed that hsa-miR-21-5p was significantly up-regulated in BC tissue, cells, and exosomes. Our results also revealed that tumor-derived hsa-miR-21-5p could be packaged in exosomes and released into peripheral blood. Additionally, when evaluating the diagnostic value of plasma exosomal hsa-miR-21-5p, we found that it was significantly up-regulated in BC patients. Receiver operating characteristic (ROC) analysis also confirmed that hsa-miR-21-5p could effectively distinguish healthy people from BC patients. The sensitivity and specificity were 86.7% and 93.3%, respectively.

Conclusion: This study's results showed that plasma exosomal hsa-miR-21-5p could be used as a biomarker for BC diagnosis.
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http://dx.doi.org/10.7717/peerj.12147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451442PMC
September 2021

Single-pixel tracking of fast-moving object using geometric moment detection.

Opt Express 2021 Sep;29(19):30327-30336

Real-time tracking of fast-moving object have many important applications in various fields. However, it is a great challenge to track of fast-moving object with high frame rate in real-time by employing single-pixel imaging technique. In this paper, we present the first single-pixel imaging technique that measures zero-order and first-order geometric moments, which are leveraged to reconstruct and track the centroid of a fast-moving object in real time. This method requires only 3 geometric moment patterns to illuminate a moving object in one frame. And the corresponding intensities collected by a single-pixel detector are equivalent to the values of the zero-order and first-order geometric moments. We apply this new approach of measuring geometric moments to object tracking by detecting the centroid of the object in two experiments. The root mean squared errors in the transverse and axial directions are 5.46 pixels and 5.53 pixels respectively, according to the comparison of data captured by a camera system. In the second experiment, we successfully track a moving magnet with a frame rate up to 7400 Hz. The proposed scheme provides a new method for ultrafast target tracking applications.
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http://dx.doi.org/10.1364/OE.436348DOI Listing
September 2021

Trans-lymphatic contrast-enhanced ultrasound with sentinel lymph node biopsy for detecting cervical skip metastasis to lymph nodes in early-stage oral tongue squamous cell carcinoma.

Dentomaxillofac Radiol 2021 Oct 6:20210107. Epub 2021 Oct 6.

Department of Oral and Maxillofacial Surgery, Fujian Provincial Hospital, 134 Dongjie, Fuzhou, China.

Objectives: The purpose of this study was to assess whether trans-lymphatic contrast-enhanced ultrasound (CEUS) combined with sentinel lymph node biopsy can be used to assess the status of cervical lymph nodes and skip metastasis in patients with early squamous cell carcinoma (SCC) of the oral tongue.

Methods: This study included 21 subjects with early oral tongue SCC who received multiple intramucosal peritumoral injections of Sonazoid. CEUS examinations were performed immediately after Sonazoid injection to identify sentinel lymph nodes (SLNs). The SLNs were excised for histological examination to determine if the lymph nodes has metastases.

Results: Thirty-five SLNs were detected by CEUS after Sonazoid injection in the subjects. SLNs were identified in 20 of the total 21 subjects. Four participants had metastasis to lymph nodes, and one had skip metastasis in level Ⅲ. In one subject, SLNs were successfully detected in level Ⅳ without skip metastasis.

Conclusions: Trans-lymphatic CEUS combined with sentinel lymph node biopsy showed high accuracy for evaluating cervical lymph node status. This could be a reliable approach for detecting cervical skip metastases of lymph nodes in early-stage oral cancer patients.
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http://dx.doi.org/10.1259/dmfr.20210107DOI Listing
October 2021

Nonlinear optical limiting effect of graphene dispersions at 1064  nm.

Appl Opt 2021 Oct;60(28):8858-8864

We study the nonlinear optical limiting effect of graphene dispersions in ethanol and acetone at a wavelength of 1064 nm. The nonlinear optical limiting effect of graphene dispersion under three different linear transmittances (about 70%, 80%, and 90%), two different thicknesses (1 and 3 cm), and two different solvents (ethanol and acetone) are measured. The influences of concentration, thickness, and solvent on the nonlinear optical limiting effect of the graphene dispersion are analyzed. The experimental results show that the concentration and solution thicknesses have great influence on the optical limiting ability of graphene dispersions. The graphene dispersions with ethanol and acetone as solvents can be used to achieve excellent nonlinear optical limiting effects. The optical limiting ability of the graphene dispersion in acetone is better than that of the graphene dispersion in ethanol.
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http://dx.doi.org/10.1364/AO.434978DOI Listing
October 2021

Integrated Analysis and Finding Reveal Anti-Liver Cancer Targets and Mechanisms of Pachyman ( Polysaccharides).

Front Pharmacol 2021 17;12:742349. Epub 2021 Sep 17.

Department of Oncology, Liuzhou Worker's Hospital, Liuzhou, China.

This bioinformatics study aimed to characterize and certify crucial anti-cancer targets, functional processes, and molecular mechanisms of Pachyman in treating hepatocellular carcinoma (HCC) by using pharmacology network and molecular docking analyses, by experimental validation. The crucial anti-HCC targets of Pachyman, including ALB, VEGFA, TNF, CASP3, SRC, EGF, CXCR4, STAT3, HRAS, HSP90AA1, MMP9, BCL2L1, FGF2, and PTPRC, were identified. In addition, the correlative networks of all crucial biotargets of Pachyman in treating HCC were created accordingly. Functionally, these crucial genes were correlated using angiogenesis and neoplastic metastasis of HCC. Interestingly, the molecular docking findings indicated that ALB and VEGFA in HCC might be potent pharmacological targets of Pachyman. In experimental validation, the clinical samples of HCC showed reduced ALB protein expression and increased VEGFA protein level. Following Pachyman treatments , the intracellular level of ALB protein was elevated, whereas the cellular content of VEGFA protein was downregulated. Taken together, current bioinformatics findings based on pharmacology network and molecular docking analyses elucidate the detailed molecular targets and signaling mechanisms of Pachyman in treating HCC. Interestingly, validated biotargets of ALB and VEGFA may be main potential biomarkers for detecting HCC medically.
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http://dx.doi.org/10.3389/fphar.2021.742349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484528PMC
September 2021

Resistance of Acta2 Mice to Aortic Disease is Associated with Defective Release of Mutant Smooth Muscle α-actin from the Chaperonin-containing TCP1 folding complex.

J Biol Chem 2021 Sep 30:101228. Epub 2021 Sep 30.

Division of Medical Genetic, Department of Internal Medicine, McGovern Medical School, University of Texas Health Science Center at Houston, TX 77030 USA. Electronic address:

Pathogenic variants of the ACTA2 gene, which encodes smooth muscle (SM) α-actin, predispose to heritable thoracic aortic disease. The ACTA2 variant p.Arg149Cys (R149C) is the most common alteration; however only 60% of carriers have a dissection or undergo repair of an aneurysm by 70 years of age. A mouse model of ACTA2 p.Arg149Cys was generated using CRISPR/Cas9 technology to determine the etiology of reduced penetrance. Acta2 mice had significantly decreased aortic contraction compared to wild type (WT) mice but did not form aortic aneurysms or dissections when followed to 24 months, even when hypertension was induced. In vitro motility assays found decreased interaction of mutant SM α-actin filaments with smooth muscle myosin. Polymerization studies using Total Internal Reflection Fluorescence (TIRF) microscopy showed enhanced nucleation of mutant SM α-actin by formin, which correlated with disorganized and reduced SM α-actin filaments in Acta2 smooth muscle cells (SMCs). However, the most prominent molecular defect was the increased retention of mutant SM α-actin in the chaperonin-containing t-complex polypeptide (CCT) folding complex, which was associated with reduced levels of mutant compared to WT SM α-actin in Acta2 SMCs. These data indicate that Acta2 mice do not develop thoracic aortic disease despite decreased contraction of aortic segments and disrupted SM α-actin filament formation and function in Acta2 SMCs. Enhanced binding of mutant SM α-actin to CCT decreases the mutant actin versus WT monomer levels in Acta2 SMCs, thus minimizing the effect of the mutation on SMC function and potentially preventing aortic disease in the Acta2 mice.
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http://dx.doi.org/10.1016/j.jbc.2021.101228DOI Listing
September 2021

China Nutrition and Health Surveys (1982-2017).

China CDC Wkly 2021 Feb;3(9):193-195

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, China.

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http://dx.doi.org/10.46234/ccdcw2021.058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393036PMC
February 2021

Iron Status Among Children Aged 6-17 Years by Serum Ferritin - China, 2016-2017.

China CDC Wkly 2021 Mar;3(11):221-225

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Key Laboratory of Trace Element Nutrition, National Health Commission of the People's Republic of China, Beijing, China.

What Is Already Known On This Topic?: Iron deficiency (ID) is the most widespread micronutrient deficiency and have several adverse effects on health. Consequences of ID among children include delayed psychomotor development and impaired cognitive performance, which makes it important to monitor the iron status of children.

What Is Added By This Report?: In this study, the serum ferritin (SF) level was 56.6 (95% CI: 56.0-57.2) ng/mL in 65,293 children aged 6-17 years old in the National Nutrition and Health Surveillance in China in 2016-2017. ID prevalence varied significantly in children stratified by sex, age, and regions ranging from 1.0% to 28.1% judged by the standard of SF<15 ng/mL and SF<25 ng/mL. ID prevalence in females aged 12-17 years was the highest among children aged 6-17 years.

What Are The Implications For Public Health Practice?: Understanding iron status of school children could provide evidence and data for developing policies and strategies for ID and iron deficiency anemia (IDA) control and prevention. Females aged 12-17 years showed high ID prevalence, and iron-rich food interventions are strongly recommended.
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http://dx.doi.org/10.46234/ccdcw2021.063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393040PMC
March 2021

Impact of Public Health Event on COVID-19 Vaccine Hesitancy on Social Media: National Infoveillance Study.

JMIR Public Health Surveill 2021 Sep 20. Epub 2021 Sep 20.

Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Room 324, 3/F, Second Social Science Building, Jinan University, Guangzhou, China, Guangzhou, CN.

Background: The ongoing Coronavirus 2019 (COVID-19) pandemic has brought unprecedented challenges to every country in the world. A call for global vaccination of COVID-19 plays a pivotal role in the fight against this virus. With the development of COVID-19 vaccines, public willingness to accept a vaccination has become an important concern of public health given the vaccine hesitancy observed in the world. Social media is powerful in monitoring public attitudes and assess the dissemination, which would provide valuable information for policy makers.

Objective: This study aimed to investigate the responses of vaccine positivity on social media when major public events (major outbreaks) or major adverse event of vaccination (COVID-19 or other similar vaccines) were reported.

Methods: A total of 340,783 vaccine-related posts was captured with the poster's information on Weibo, the biggest social platform in China. After data cleaning, 156,223 posts were included in the subsequent analysis. By using pandas and SnowNLP Python libraries, posts were classified into 2 categories, positive and negative. After model training and sentiment analysis, the proportion of positive posts was computed to measure the public positivity toward the COVID-19 vaccine.

Results: The positivity of COVID-19 vaccine in China tends to fluctuate over time in the range of 45.7% to 77.0% and is intuitively correlated with public health events. In terms of gender, males were more positive (70.0% of the time) than females. In terms of region, when regional epidemics arose, not only the region with the epidemic and surrounding regions but even the whole country showed more positive attitudes to varying degrees. When the epidemic subsided temporarily, positivity decreased with varying degrees in each region.

Conclusions: In China, public positivity of COVID-19 vaccines fluctuates over time and a regional epidemic or news on social media may cause significant variations in willingness to accept a vaccination. Furthermore, public attitudes of COVID-19 vaccination vary from gender and region. It is crucial for policy makers to adjust their policies through the use of positive incentives with prompt responses to pandemic-related news to promote vaccination acceptance.

Clinicaltrial:
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http://dx.doi.org/10.2196/32936DOI Listing
September 2021

Understanding Health Communication Through Google Trends and News Coverage for COVID-19: A Multinational Study in Eight Countries.

JMIR Public Health Surveill 2021 Sep 18. Epub 2021 Sep 18.

Communication Department, University of Albany, State University of New York, Albany, NY United States, School of Journalism and Communication, National Media Experimental Teaching Demonstration Center (Jinan University), Jinan University, Guangzhou, China, 601 Huangpu Dadao West, Guangzhou City, China, Guangzhou, CN.

Background: Due to the COVID-19 pandemic, health information related to COVID-19 has spread across the news media worldwide. Google is among the most used Internet search engines, and the Google Trends tool can reflect how the public seek COVID-related health information during this period.

Objective: To understand health communication through Google Trends and news coverage and to explore their relationship with prevention and control of COVID-19 in the early epidemic stage.

Methods: To achieve the study objectives, we analyzed the public's information-seeking behaviors on Google and news media coverage on COVID-19. We collected data on COVID-19 news coverage and Google search queries for eight countries (United States, United Kingdom, Canada, Singapore, Ireland, Australia, South Africa, and New Zealand) between January 1, 2020 and April 29, 2020, and depicted the characteristics of the trend of news coverage on COVID-19 over time, as well as the search queries trends on the topics of COVID-19 related diseases, treatments and medical resources, symptoms and signs, and public measures. And the search queries trend provided relative search volume (RSV) as an indicator to represent the popularity of a specific search term in a specific geographic area over a period. Also, time-lag correlation analysis was used to further explore the relationship between the trend of search terms and the number of daily new cases, the relationship between search terms trends and news coverage.

Results: Across all search trends in eight countries, almost all search peaks were formed between March and April 2020, and declined occurred in April 2020. Regarding COVID-19 related diseases, in most countries, the RSV of "coronavirus" increased earlier than "covid-19", but around April 2020, the search volume of "covid-19" surpassed "coronavirus". For treatments and medical resources, the most and least searched terms were "mask" and "ventilator", respectively. In the topic of symptoms and signs, "fever" and "cough" were the most searched terms. And the RSV of "lockdown" was significantly more than "social distancing" on the topic of public measures. Besides, when combining the search trends with news coverage, there were three main patterns: the United States pattern, the Singapore pattern, and the other-countries pattern. In the time-lag correlation analysis between the RSV of the topic "Treatment and medical resources" and the number of daily new cases, except for Singapore, the RSV of this topic were positively correlated with daily new cases in other countries, with the maximum correlation of 0.8 (United States). And in the time-lag correlation analysis between the overall RSV of "Diseases" and the number of daily news, the overall RSV of this topic were positively correlated with the number of daily news, the maximum correlation coefficient was more than 0.8, and the search behavior was 0-17 days earlier than the news coverage.

Conclusions: Our findings revealed public interest in masks, disease control, and public health measures, and revealed the potential value of Google Trends in the face of the emergence of new infectious diseases. Also, Google Trends combined with news media can achieve more efficient health communication. Therefore, both news media and Google Trends contribute to the early prevention and control of the epidemic.

Clinicaltrial:
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http://dx.doi.org/10.2196/26644DOI Listing
September 2021

Bilateral lung transplantation for Castleman disease with end-stage bronchiolitis obliterans.

Clin Transplant 2021 Sep 30:e14496. Epub 2021 Sep 30.

Wuxi Lung Transplant Center, Department of Thoracic Surgery, Wuxi People's Hospital affiliated to Nanjing Medical University, Wuxi, China.

Bronchiolitis obliterans (BO) is a severe complication of Castleman disease (CD), a rare lymphoproliferative disease with unclear pathogenesis. Currently, there are no reports on the safety or outcomes of bilateral lung transplantation in patients with BO due to CD. This study aimed to characterize the clinical manifestations and features of BO and CD. We retrospectively analyzed the medical records of six consecutive patients with BO and CD who underwent bilateral lung transplantation between December 2012 and December 2020. The average age of patients at lung transplantation was 33 ± 15 years, and the age range of patients at diagnosis of CD was about 9-56 years. The body mass index was 15.2 ± 1.9 kg/m . The average time from diagnosis to lung transplantation was 4.1 ± 2.7 years. All the patients had unicentric CD (UCD); five had concomitant paraneoplastic pemphigus, and four received extracorporeal membrane oxygenation during surgery. The average hospital stay was 51 ± 53 days. Infection was the most common postoperative complication. CD did not recur in any of the patients. Thus, bilateral lung transplantation is a viable and safe treatment for selected patients with CD and BO, which can improve the quality of life and prolong survival.
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http://dx.doi.org/10.1111/ctr.14496DOI Listing
September 2021

Correlation of genotype and phenotype in 32 patients with hereditary hemochromatosis in China.

Orphanet J Rare Dis 2021 09 28;16(1):398. Epub 2021 Sep 28.

Beijing Key Laboratory of Translational Medicine On Liver Cirrhosis , Liver Research Center, Beijing Friendship Hospital, Capital Medical University, 95 Yong-An Road, Beijing, 100050, China.

Background: Hereditary hemochromatosis (HH) is widely recognized and clinical manifestations of hemochromatosis-related (HFE-related) HH is well studied in European populations. Less is known about the clinical and laboratory characteristics of non-HFE related HH in Asian population. We aimed to explore the relationship between genotype and clinical phenotype in Chinese patients with non-HFE related hereditary hemochromatosis.

Methods: Peripheral blood samples and clinical data of patients with primary iron overload were collected from the China Registry of Genetic/Metabolic Liver Diseases. Sanger sequencing was performed in cases with primary iron overload, for 5 known HH related genes (HFE, HJV, HAMP, TFR2 and SLC40A1) and 2 novel iron homeostasis-related genes (DENND3 and SUGP2). The correlation of genotype and clinical phenotype in these patients was analyzed.

Results: Of the 32 patients with primary iron overload (23 were males and 9 were females), non-HFE variants were detected in 31 (31/32, 97%), including 8 pathogenic variants in HJV, 7 pathogenic variants in SLC40A1, 8 likely pathogenic variants in SUGP2 and 5 likely pathogenic variants in DENND3 cases. Among these 31 cases, 4 cases harbored homozygous variants, 2 cases harbored homozygous + heterozygous variants, 19 cases harbored heterozygous or combined heterozygous variants, and 6 cases harbored no any damaging variants. None of investigated cases carried damaging HAMP and TFR2 variants were found. 8 cases were classified as type 2A HH and 6 cases as type 4 HH, 10 cases as non-classical genotype, and 6 cases had no pathogenic variants from 31 cases. During the statistical analysis, we excluded one case (SLC40A1 IVS3 + 10delGTT + SUGP2 p. R639Q(homo)) with difficulty in grouping due to combined damaging variants. Cases with type 2A HH have an earlier age at diagnosis (p = 0.007). The iron index of cases in type 2A HH and type 4 HH was higher than that in other groups (p = 0.01). Arthropathy was relatively rare in all groups. None of cases with type 2A HH developed cirrhosis. Cirrhosis and diabetes are more prevalent in type 4 HH. The incidence of cirrhosis (p = 0.011), cardiac involvement (p = 0.042), diabetes (p = 0.035) and hypogonadism (p = 0.020) was statistically significant in the four groups. However, due to the limited sample size, the pairwise comparison showed no significant difference.

Conclusions: This is the first comprehensive analysis about the gene variant spectrum and phenotypic aspects of non-HFE HH in China. The results will be useful to the identification, diagnosis and management of HH in China.
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http://dx.doi.org/10.1186/s13023-021-02020-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8479922PMC
September 2021

Incidence and Outcomes of Infective Endocarditis After Transcatheter or Surgical Aortic Valve Replacement.

J Am Heart Assoc 2021 Oct 28;10(19):e020368. Epub 2021 Sep 28.

Department of Cardiology InselspitalBern University Hospital Bern Switzerland.

Background Data comparing the frequency and outcomes of infective endocarditis (IE) after transcatheter (TAVR) to surgical aortic valve replacement (SAVR) are scarce. The objective of this study is to compare the incidence and outcomes of IE after TAVR using a supra-annular, self-expanding platform (CoreValve and Evolut) to SAVR. Methods and Results Data of 3 randomized clinical trials comparing TAVR to SAVR and a prospective continued TAVR access study were pooled. IE was defined on the basis of the modified Duke criteria. The cumulative incidence of IE was determined by modeling the cause-specific hazard. Estimates of all-cause mortality were calculated by means of the Kaplan-Meier method. Outcomes are reported for the valve-implant cohort. During a mean follow-up time of 2.17±1.51 years, 12 (0.5%) of 2249 patients undergoing TAVR and 21 (1.1%) of 1828 patients undergoing SAVR developed IE. Patients with IE more frequently had diabetes mellitus than those without (57.6% versus 34.2%; =0.005). The cumulative incidence of IE was 1.01% (95% CI, 0.47%-1.96%) after TAVR and 1.58% (95% CI, 0.97%-2.46%) after SAVR (=0.047) at 5 years. Among patients with IE, the rate of all-cause mortality was 27.3% (95% CI, 1.0%-53.6%) in the TAVR and 51.8% (95% CI, 28.2%-75.3%) in the SAVR group at 1 year (log-rank =0.15). Conclusions Pooled prospectively collected data comparing TAVR with a supra-annular, self-expanding device to SAVR showed a low cumulative risk of IE irrespective of treatment modality, although the risk was lower in the TAVR implant group. Once IE occurred, mortality was high. Registration URL: https://www.clinicaltrials.gov; Unique identifiers: NCT01240902, NCT01586910, NCT02701283.
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http://dx.doi.org/10.1161/JAHA.120.020368DOI Listing
October 2021

Molecular Characterization and Cross-Reactivity of Feline Calicivirus Circulating in Southwestern China.

Viruses 2021 Sep 12;13(9). Epub 2021 Sep 12.

College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China.

Feline calicivirus (FCV) is an important pathogen of cats that has two genogroups (GI and GII). To investigate the prevalence and molecular characteristics of FCVs in southwestern China, 162 nasal swab samples were collected from cats in animal shelters and pet hospitals. In total, 38 of the clinical samples (23.46%) were identified as FCV positive using nested RT-PCR. Phylogenetic analyses using 10 capsid protein VP1 sequences revealed that 8 GI and 2 GII strains formed two independent clusters. Additionally, three separated FCVs that were not clustered phylogenetically (two GI and one GII strains) were successfully isolated from clinical samples and their full-length genomes were obtained. Phylogenetic and recombinant analyses of a GI FCV revealed genomic breakpoints in ORF1 and ORF2 regions with evidence for recombinant events between GI sub-genogroups, which is reported in China for the first time. Furthermore, sera obtained from mice immunized independently with the three FCV isolates and a commercial vaccine were used to evaluate the cross-reactivity of neutralizing antibodies. The three separate FCVs were neutralized by each other at a 1:19 to 1:775 titer range, whereas the triple-inactivated vaccine was at a titer of 1:16, which suggested that different genogroup/sub-genogroup FCV strains exhibit significantly different titers of neutralizing antibodies, including the commercial FCV vaccine. Thus, our study revealed the genetic diversity and complex cross-reactivity levels of FCVs in southwestern China, which provides new insights for application in vaccination strategies.
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http://dx.doi.org/10.3390/v13091812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8473038PMC
September 2021

Human umbilical cord mesenchymal stem cells prevent glucocorticoid-induced osteonecrosis of the femoral head by promoting angiogenesis.

J Plast Surg Hand Surg 2021 Sep 27:1-7. Epub 2021 Sep 27.

Department of Orthopedics, Weihai Central Hospital, Affiliated to Qingdao University & Weihai Key Laboratory of Autoimmunity, Qingdao University, Weihai, China.

The impairment of angiogenesis is an outstanding pathogenic characteristic of glucocorticoid (GC)-induced osteonecrosis of the femoral head (ONFH). Human umbilical cord mesenchymal stem cells (hUC-MSCs) have been used in several diseases models, which were reported to be involved in the angiogenesis. However, whether hUC-MSCs suppress the GC-induced ONFH promoting angiogenesis is still unclear. hUC-MSCs were isolated from the Wharton's jelly using the explant culture method. A GC-induced ONFH model was established and . The angiogenesis, proliferation and migration ability of HMECs were determined using the tube-forming, CCK-8, transwell and scratching assays . The protective role of hUC-MSCs in GC-induced ONFH was evaluated using micro-CT scanning and histological, immunohistochemical (IHC) and Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays . The results showed that hUC-MSCs treatment improved the tube-forming, proliferation and migration ability of HMECs . Moreover, hUC-MSCs treatment enhanced the integrity of trabecular bone of the femoral head, and the tube-forming ability . hUC-MSCs prevent the femoral head against necrosis and damage caused by GCs though promoting angiogenesis.
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http://dx.doi.org/10.1080/2000656X.2021.1981352DOI Listing
September 2021
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