Publications by authors named "Jian Ge"

376 Publications

Development of a Nomogram for Moderate-to-Severe Bronchopulmonary Dysplasia or Death: Role of N-Terminal Pro-brain Natriuretic Peptide as a Biomarker.

Front Pediatr 2021 23;9:727362. Epub 2021 Aug 23.

Neonatal Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

This study aimed to explore the clinical value of N-terminal pro-brain natriuretic peptide (NT-proBNP) in predicting moderate-to-severe bronchopulmonary dysplasia (BPD)/death, and to establish an effective clinical predictive nomogram. We retrospectively analyzed very low birth weight infants (VLBWs) with gestational age ≤ 32 weeks. The NT-proBNP values were determined on the 1st, 3rd, 7th, 14th, 21st, and 28th days after birth. The correlation between NT-proBNP level and moderate-to-severe BPD/death was evaluated. Receiver operating characteristic (ROC) curve analysis was used to evaluate the prediction ability. Then, we used multivariable logistic regression to build the prediction model and nomogram, and calibration of the model was assessed by calibration curve. In total, 556 VLBWs were involved, among whom 229 developed BPD (mild: = 109; moderate: = 68; severe: = 52) and 18 died. The NT-proBNP level in the moderate-to-severe BPD/death group was significantly higher than that in the no-to-mild BPD group from the 3rd to 28th day ( < 0.001). When the natural logarithm of the serum NT-ProBNP level increased by 1 unit at day 7 (±2 days) of life, the risk of moderate and severe BPD/death was the highest (OR = 3.753; 95% CI: 2.984~4.720), and ROC analysis identified an optimal cutoff point of 3360 ng/L (sensitivity: 80.0%; specificity: 86.2%; AUC: 0.861). After adjusting for confounding factors, the level of NT-proBNP at day 7 (±2 days) of life still had important predictive value for the development of moderate-to-severe BPD/death, significantly improving the predictive ability of the model. The level of NT-proBNP at day 7 (±2 days) of life can be used as an early promising biomarker for VLBWs to develop moderate-to-severe BPD/death. We constructed an early predictive nomogram to help clinicians identify high-risk populations.
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http://dx.doi.org/10.3389/fped.2021.727362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419419PMC
August 2021

A modular magneto-inductive sensor for low vector magnetic field measurements.

Rev Sci Instrum 2021 Aug;92(8):085110

School of Engineering, University of British Columbia Okanagan Campus, Kelowna, British Columbia V1V 1V7, Canada.

The low magnetic field measurement has been utilized since ancient times in order to find economic resources, to detect magnetic anomalies, etc. In this case, the vector magnetic survey can simultaneously obtain the modulus and direction information of the magnetic field, which can contribute to obtaining more precise information and characteristics of magnetic field resources. This paper is concerned with the potential to exploit the signals of vector magnetic field measurement with a magneto-inductive (MI) sensor. To evaluate the capability of the MI sensor, a test platform is set up and its performance, including the noise floor, the resolution, and the sensitivity, is comprehensively characterized. Furthermore, a comparative geomagnetic observation and magnetic anomaly detection among the proposed MI sensor, a high-precision Overhauser sensor, and a commonly used and accepted commercial MI sensor are conducted. The experimental results identify the capability of the proposed MI sensor in weak magnetic detection.
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http://dx.doi.org/10.1063/5.0063450DOI Listing
August 2021

Carbon Nanotube Polymer Scaffolds as a Conductive Alternative for the Construction of Retinal Sheet Tissue.

ACS Chem Neurosci 2021 09 10;12(17):3167-3175. Epub 2021 Aug 10.

State Key Laboratory of Ophthalmology, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, China.

With the great success of graphene in the biomedical field, carbon nanotubes have attracted increasing attention for different applications in ophthalmology. Here, we report a novel retinal sheet composed of carbon nanotubes (CNTs) and poly(lactic--glycolic acid) (PLGA) that can enhance retinal cell therapy. By tuning our CNTs to regulate the mechanical characteristics of retina sheets, we were able to improve the in vitro viability of retinal ganglion cells derived from human-induced pluripotent stem cells incorporated into CNTs. Engrafted retinal ganglion cells displayed signs of regenerating processes along the optic nerve. Compared with PLGA scaffolds, CNT-PLGA retinal sheet tissue has excellent electrical conductivity, biocompatibility, and biodegradation. This new biomaterial offers new insight into retinal injury, repair, and regeneration.
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http://dx.doi.org/10.1021/acschemneuro.1c00242DOI Listing
September 2021

Case Report: Local Cytokine Release Syndrome in an Acute Lymphoblastic Leukemia Patient After Treatment With Chimeric Antigen Receptor T-Cell Therapy: A Possible Model, Literature Review and Perspective.

Front Immunol 2021 19;12:707191. Epub 2021 Jul 19.

Department of Hematology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Chimeric antigen receptor T (CAR-T) cell therapy has achieved remarkable clinical efficacy in treatment of many malignancies especially for B-cell hematologic malignancies. However, the application of CAR-T cells is hampered by potentially adverse events, of which cytokine release syndrome (CRS) is one of the severest and the most studied. Local cytokine-release syndrome (L-CRS) at particular parts of the body has been reported once in a while in B-cell lymphoma or other compartmental tumors. The underlying mechanism of L-CRS is not well understood and the existing reports attempting to illustrate it only involve compartmental tumors, some of which even indicated L-CRS only happens in compartmental tumors. Acute lymphoblastic leukemia (ALL) is systemic and our center treated a B-cell ALL patient who exhibited life threatening dyspnea, L-CRS was under suspicion and the patient was successfully rescued with treatment algorithm of CRS. The case is the firstly reported L-CRS related to systemic malignancies and we tentatively propose a model to illustrate the occurrence and development of L-CRS of systemic malignancies inspired by the case and literature, with emphasis on the new recognition of L-CRS.
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http://dx.doi.org/10.3389/fimmu.2021.707191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326907PMC
July 2021

Biodegradable scaffolds facilitate epiretinal transplantation of hiPSC-Derived retinal neurons in nonhuman primates.

Acta Biomater 2021 Jul 24. Epub 2021 Jul 24.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong 510060, China. Electronic address:

Transplantation of stem cell-derived retinal neurons is a promising regenerative therapy for optic neuropathy. However, significant anatomic differences compromise its efficacy in large animal models. The present study describes the procedure and outcomes of human-induced pluripotent stem cell (hiPSC)-derived retinal sheet transplantation in primate models using biodegradable materials. Stem cell-derived retinal organoids were seeded on polylactic-coglycolic acid (PLGA) scaffolds and directed toward a retinal ganglion cell (RGC) fate. The seeded tissues showed active proliferation, typical neuronal morphology, and electrical excitability. The cellular scaffolds were then epiretinally transplanted onto the inner surface of rhesus monkey retinas. With sufficient graft-host contact provided by the scaffold, the transplanted tissues survived for up to 1 year without tumorigenesis. Histological examinations indicated survival, further maturation, and migration. Moreover, green fluorescent protein-labeled axonal projections toward the host optic nerve were observed. Cryopreserved organoids were also able to survive and migrate after transplantation. Our results suggest the potential efficacy of RGC replacement therapy in the repair of optic neuropathy for the restoration of visual function. STATEMENT OF SIGNIFICANCE: In the present study, we generated a human retinal sheet by seeding hiPSC-retinal organoid-derived RGCs on a biodegradable PLGA scaffold. We transplanted this retinal sheet onto the inner surface of the rhesus monkey retina. With scaffold support, donor cells survive, migrate and project their axons into the host optic nerve. Furthermore, an effective cryopreservation strategy for retinal organoids was developed, and the thawed organoids were also observed to survive and show cell migration after transplantation.
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http://dx.doi.org/10.1016/j.actbio.2021.07.040DOI Listing
July 2021

A Good Way to Reduce Screening for Retinopathy of Prematurity: Development of the ROP Model in a China Preterm Population.

Front Pediatr 2021 30;9:697690. Epub 2021 Jun 30.

Neonatal Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Retinopathy of prematurity (ROP) is a preventable cause of blindness in children. Without treatment, more than 45% of eyes may suffer permanent vision loss. Current ROP screening guidelines, which include a range of birth weights (BWs) and gestational ages (GAs), may require screening many low-risk preemies who might develop severe ROP. All high-risk infants in the neonatal intensive care unit (NICU) of the First Affiliated Hospital of Zhengzhou University from 2017 to 2021 were included in this retrospective cohort study. Each of the 27 candidate risk factors was evaluated in univariate analysis and adjusted for known risk factors (i.e., GA and BW). The significant results were analyzed in a backward selection multivariate logistic regression model. Receiver operating characteristic (ROC) curves and a nomogram were drawn. The study included 2,040 infants who underwent ROP screening. The weight gain rate [OR, 2.65; 95% confidence interval (CI), 1.49-1.21 ≤ 12 g/d vs. > 18 g/d; = 0.001], blood transfusion (OR, 2.03; 95% CI, 1.14-3.64; = 0.017), invasive mechanical ventilation (OR, 1.74; 95% CI, 1.15-2.66; = 0.009) and N-terminal segment of pro-B-type natriuretic peptide (NT-proBNP) ≥ 25,000 ng/L (OR, 1.51; 95% CI, 1.00-2.28; = 0.048) were four new statistically independent risk factors in addition to GA and BW. The area under the curve (AUC) of the final multivariate model was 0.90 (95% CI, 0.88-0.92; < 0.001). These findings add to our understanding of ROP screening because they include all eligible infants rather than only high-risk infants, as in previous studies. Under the control of BW and GA, low weight gain rate, increased number of blood transfusion, invasive mechanical ventilation and NT-proBNP ≥ 25,000 ng/L were "new" statistically independent risk factors for ROP. The ROP risk can be calculated manually or represented by a nomogram for clinical use.
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http://dx.doi.org/10.3389/fped.2021.697690DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278052PMC
June 2021

An optimized free induction decay signal sensing coil and its matching circuit for miniaturized Overhauser geomagnetic sensor.

Rev Sci Instrum 2021 May;92(5):055005

Science and Technology on Near-Surface Detection Laboratory, Wuxi 214035, China.

An Overhauser geomagnetic sensor is a precise instrument commonly employed for geomagnetic field observation, magnetic surveys, and so on. Currently, the miniaturization of the Overhauser geomagnetic sensor is limited due to the lower signal-to-noise ratio. Thus, how to effectively extract weaker free induction decay (FID) signal from a miniaturized sensor and how to improve the signal quality have become the bottleneck. To address these problems, we came up with an optimal design of the FID signal sensing coil for a miniaturized Overhauser geomagnetic sensor and propose a front-end matching circuit for the sensing coil to inhibit the attenuation of the signal amplitude caused by high impedance, further reducing the overall noise floor of the signal acquisition system. Finally, the field experimental results show that the miniaturized prototype sensor has a smaller volume and mass with an approximate performance compared with the commercial sensor.
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http://dx.doi.org/10.1063/5.0032431DOI Listing
May 2021

Retrograde fluorogold labeling of retinal ganglion cells in neonatal mice.

Ann Transl Med 2021 May;9(10):878

State Key Laboratory of Ophthalmology, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Background: The neonatal period, especially postnatal day 10 (P10), is important for mouse retinal ganglion cells (RGCs) development, and an effective labeling technique to track neonatal RGCs is needed. Retrograde fluorogold (FG) labeling is widely used for adult mouse RGCs, but its applicability for the neonatal mouse is still unknown. This study aimed to evaluate the safety and efficiency of retrograde FG labeling in P10 mice.

Methods: The anatomic location of the superior colliculus (SC) of P10 wild-type C57/BL6J mice was clarified by histological brain section and hematoxylin and eosin (H&E) staining. Three doses of 3% FG were injected into the SC of 30 mice, and 3 days post-surgery, labeling efficiency was quantified by retinal flat-mounts, and labeling safety was evaluated by mice mortality.

Results: Samples of brain tissue from 2-3.5 mm posterior to the bregma, and from 0.5-2.0 mm lateral to the midline showed major SC-related structures. The FG-positive RGC density in the 0.3 µL group was 3,563.9±311.9 cells/mm, significantly more than in the 0.6 µL group (1,718.6±177.1 cells/mm) or 1.0 µL group (2,496.8±342.2 cells/mm). The mortality rate was 10% in both the 0.3 and 0.6 µL groups, but 40% in the 1.0 µL group.

Conclusions: The appropriate labeling site in P10 mice was confirmed and 0.3 µL FG is an appropriate dose for retrograde labeling of RGCs.
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http://dx.doi.org/10.21037/atm-21-2022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184436PMC
May 2021

Dual Effects of Cyclooxygenase Inhibitors in Combination With CD19.CAR-T Cell Immunotherapy.

Front Immunol 2021 26;12:670088. Epub 2021 May 26.

Department of Internal Medicine V, University Clinic Heidelberg, Heidelberg University, Heidelberg, Germany.

Chimeric antigen receptor T (CAR-T) cells targeting CD19 came into clinical practice for the treatment of B cell lymphoma in 2018. However, patients being treated for B cell lymphoma often suffer from comorbidities such as chronic pain, cardiovascular diseases and arthritis. Thus, these patients frequently receive concomitant medications that include nonsteroidal anti-inflammatory drugs (NSAIDs) like cyclooxygenase (COX) inhibitors. Celecoxib, a selective COX-2 inhibitor, and aspirin, a non-selective COX-1 and COX-2 inhibitor, are being used as anti-inflammatory, analgesic and anti-pyretic drugs. In addition, several studies have also focused on the anti-neoplastic properties of COX-inhibitors. As the influence of COX-inhibitors on CD19.CAR-T cells is still unknown, we investigated the effect of celecoxib and aspirin on the quantity and quality of CD19.CAR-T cells at different concentrations with special regard to cytotoxicity, activation, cytokine release, proliferation and exhaustion. A significant effect on CAR-T cells could be observed for 0.1 mmol/L of celecoxib and for 4 mmol/L of aspirin. At these concentrations, we found that both COX-inhibitors could induce intrinsic apoptosis of CD19.CAR-T cells showing a significant reduction in the ratio of JC-10 red to JC-10 green CAR-T cells from 6.46 ± 7.03 (mean ± SD) to 1.76 ± 0.67 by celecoxib and to 4.41 ± 0.32 by aspirin, respectively. Additionally, the ratios of JC-10 red to JC-10 green Daudi cells were also decreased from 3.41 ± 0.30 to 0.77 ± 0.06 by celecoxib and to 1.26 ± 0.04 by aspirin, respectively. Although the cytokine release by CD19.CAR-T cells upon activation was not hampered by both COX-inhibitors, activation and proliferation of CAR-T cells were significantly inhibited diminishing the NF-ĸB signaling pathway by a significant down-regulation of expression of CD27 on CD4 and CD8 CAR-T cells, followed by a clear decrease of phosphorylated NF-ĸB p65 in both CD4 and CD8 CAR-T cells by a factor of 1.8. Of note, COX-inhibitors hampered expansion and induced exhaustion of CAR-T cells in an antigen stress assay. Collectively, our findings indicate that the use of COX-inhibitors is a double-edged sword that not only induces apoptosis in tumor cells but also impairs the quantity and quality of CAR-T cells. Therefore, COX-inhibitors should be used with caution in patients with B cell lymphoma under CAR-T cell therapy.
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http://dx.doi.org/10.3389/fimmu.2021.670088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189155PMC
May 2021

Clinical Significance of Hyperdense Area after Endovascular Therapy in Patients with Acute Ischemic Stroke: A Systematic Review and Meta-Analysis.

Cerebrovasc Dis 2021 27;50(5):500-509. Epub 2021 May 27.

Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Objective: We performed a systematic review and meta-analysis to investigate the clinical significance of hyperdense area after thrombectomy in patients with acute ischemic stroke (AIS).

Methods: We searched Ovid MEDLINE(R) and Epub Ahead of Print, In-Process and other Non-Indexed, Cochrane Library Clinical Controlled Trials and Embase from inception to September 2020 and collected the cohort and case-control studies about the clinical significance of hyperdense area on different types of computed tomography (CT) after thrombectomy in patients with AIS. Outcomes were poor functional outcome (modified Rankin Scale [mRS] Score 3-6 at discharge or 90-day), mortality and subtypes of hemorrhage according to the European Cooperative Acute Stroke Study (ECASS).

Results: 1,999 patients from 16 studies were included in this meta-analysis. Pooled results indicated higher risk of symptomatic intracerebral hemorrhage (odds ratio [OR] = 3.02; 95% confidence interval [CI] 1.84-4.95; p < 0.0001, I2 = 0%) in patients with hyperdense area, and the subtype of parenchymal hematoma as well. There was also higher odds of poor functional outcome based on the mRS 3-6 at discharge or 90-day (OR = 1.92; 95% CI 1.35-2.73; p = 0.0003, I2 = 31%) and mortality (OR = 2.06; 95% CI 1.41-3.02; p = 0.0002, I2 = 0%) in patients with hyperdense area after thrombectomy compared with those without hyperdense area.

Conclusions: Our results indicated that the presence of hyperdense area on CT after thrombectomy was associated with high risk of symptomatic intracerebral hemorrhage, poor functional outcome, as well as mortality in patients with AIS. However, further studies were needed to confirm these results. The meta-analysis was conducted in adherence with the PRISMA Statement and was registered at the International Prospective Register of Systematic Reviews (CRD42020164165). To the best of our knowledge, this study is the first meta-analysis investigating the effect of hyperdense area after endovascular therapy in patients with AIS.
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http://dx.doi.org/10.1159/000515410DOI Listing
May 2021

Enhanced migration of engrafted retinal progenitor cells into the host retina via disruption of glial barriers.

Mol Vis 2021 10;27:300-308. Epub 2021 May 10.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Purpose: Migration and integration remain critical challenges for stem cell replacement therapy. Glial barriers play an important role in preventing cell migration and integration. The purpose of this study was to investigate the effect and mechanisms of chondroitinase ABC on the migration of murine retinal progenitor cells (mRPCs) transplanted into the subretinal space of B6 mice.

Methods: mRPCs were harvested from the neural retinas of P1 enhanced green fluorescent protein (GFP) B6 mice. Two μl containing 2 × 10 expanded RPCs alone or combined with chondroitinase ABC in suspension were injected into the subretinal space of the recipient B6 mice. Immunohistochemistry was performed on the recipient B6 retinas to evaluate the glial barrier formation and migration of the mRPCs. Western blotting was also used to check the expression of the glial barriers.

Results: Glial fibrillary acidic protein (GFAP) and vimentin could be seen around the transplanted mRPCs in the B6 mice. Formation of glial barriers prevented the migration of donor cells into the retinal layers. Chondroitinase ABC promoted the migration and survival rates of the engrafted retinal progenitor cells in the retinal layers of recipient B6 mice. Injection induced upregulation of GFAP, chondroitin, and CD44 expression. Chondroitinase ABC disrupted the glial barriers. The CD44 around the mRPCs was much lower in the chondroitinase group. However, the CD44 in the retinal layers was considerably higher in the chondroitinase group. With the employment of chondroitinase ABC, more cells migrated into the outer nuclear layer or inner nuclear layer. The chondroitin and CD44 expression decreased 3 weeks after transplantation in the chondroitinase ABC group.

Conclusions: Chondroitinase ABC degraded glial barriers and enhanced the migration of transplanted mouse retinal progenitor cells. Chondroitinase ABC may also have induced activation of the CD44 signaling pathway to exert the effect.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131176PMC
May 2021

Effects of temperature, dissolved oxygen, and their interaction on the growth performance and condition of rainbow trout (Oncorhynchus mykiss).

J Therm Biol 2021 May 3;98:102928. Epub 2021 Apr 3.

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, Shandong Province, 266100, China.

The individual effects of temperature and dissolved oxygen (DO) on rainbow trout (Oncorhynchus mykiss), an important aquaculture species, are clearly established; however, little is known about the interactive effects of these parameters. In this study, the effects of temperature, DO, and their interaction on the growth, antioxidant status, digestive enzyme activity, serum biochemical parameters, and liver IGF-1 expression in rainbow trout were evaluated. Fish (initial weight, 109.98 ± 3.28 g) were reared in a recirculating system for 4 weeks and subjected to 6 treatments at three temperatures (13 °C, 17 °C, and 21 °C) and two DO contents (4.2 mg L and 9.6 mg L). Physiological parameters were determined at the end of the trial. Specific growth rate and feed consumption were the highest at 17 °C and the lowest at 21 °C. Additionally, lysozyme, trypsin, lipase, and amylase activities, serum glucose and serum triglyceride contents, and IGF-1 expression decreased significantly at 21 °C and total serum protein and albumin contents were significantly higher at 21 °C than at 13 °C and 17 °C, indicating that high temperature impaired the immunity, digestion, and growth of rainbow trout. However, the adverse effects of high temperature can be alleviated by a high DO content, as evidenced by the smaller increments and decrements of these parameters under hyperoxic conditions than under hypoxic conditions. In response to high temperature stress, an increase in antioxidant enzyme activity led to the removal of oxygen free radicals under hyperoxic conditions; however, this increase was inhibited under hypoxia. Our results indicated that high temperatures have adverse effects on rainbow trout, and these harmful effects can be reduced by a high DO content.
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http://dx.doi.org/10.1016/j.jtherbio.2021.102928DOI Listing
May 2021

Mechanical thrombectomy versus medical care alone in large ischemic core: An up-to-date meta-analysis.

Interv Neuroradiol 2021 May 14:15910199211016258. Epub 2021 May 14.

Department of Neurology and Suzhou Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Objective: We compared outcomes and adverse events of thrombectomy versus medical management in acute ischemic stroke (AIS) patients with baseline large infarct core.

Methods: We searched Ovid MEDLINE(R) ALL, Cochrane Library Clinical Controlled Trials and EMBASE from inception to January 2021 for studies comparing thrombectomy and medical management alone in AIS patients who had ASPECTS <=7 or ischemic core volume >=50 ml. Imaging modalities to valuate ASPECTS and core volume were without restriction. The functional outcome was measured by mRS (modified Rankin Scale) score 0-2 at 90 days or discharge. The safety end point included the rates of mortality and sICH (symptomatic intracranial hemorrhage) or PH2 (parenchymal hematoma type 2).

Results: Fourteen studies with a total of 2547 patients (thrombectomy n = [1197]; medical care alone [n = 1350]) fulfilled our criteria. As for patients with low ASPECTS, pooled results indicated a higher odds of good functional outcome (OR = 3.47; 95% CI 1.99 to 6.07; P < 0.0001, I=66%) and a lower risk of mortality (OR = 0.62; 95% CI 0.46 to 0.83; P = 0.001, I=32%) in thrombectomy group compared with no thrombectomy group, but the risk of sICH or PH2 did not differ between two groups. As for patients with large core volume, both functional outcome and safety end point between two groups showed no statistically significant difference.

Conclusion: Thrombectomy remained safe and effective by careful selection in patients with low ASPECTS. More studies were warranted to explore contraindications for mechanical thrombectomy in AIS patients, especially in patients with large core volume.
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http://dx.doi.org/10.1177/15910199211016258DOI Listing
May 2021

Myelin regulatory factor deficiency is associated with the retinal photoreceptor defects in mice.

Vis Neurosci 2021 05 3;38:E005. Epub 2021 May 3.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Previously, we reported the myelin regulatory factor (MYRF) as a candidate gene for nanophthalmos. We have also produced Myrf knockdown (Myrf+/-) mouse strain to investigate the cellular and molecular phenotypes of reduced MYRF expression in the retina. Myrf+/- mouse strain was generated using the CRISPR/Cas9 system. Optomotor response system, electroretinogram (ERG), spectral-domain optical coherence tomography (SD-OCT), histology, and immunohistochemistry were performed to evaluate retinal spatial vision, electrophysiological function, retinal thickness, and pathological changes in cone or rod photoreceptors, respectively. RNA sequencing (RNA-seq) was performed to investigate the underlying molecular mechanism linking Myrf deficiency with photoreceptor defects. The genotype and phenotype of CRISPR/Cas9-induced Myrf+/- mice and their offspring were comprehensively investigated. Photoreceptor defects were detected in the retinas of Myrf+/- mice. Visual acuity and ERG responses were decreased in Myrf+/- mice compared with the control mice (Myrf+/+). The loss of cone and rod neurons was proportional to the decreased outer nuclear layer (ONL) thickness. Moreover, RNA-seq revealed that phototransduction and estrogen signaling pathways played important roles in the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Myrf+/- mouse strain provides a good model to investigate the function of the MYRF gene. Photoreceptor defects with impaired functions of spatial vision and retinal electrophysiology indicate an important role played by MYRF in retinal development. Alterations in phototransduction and estrogen signaling pathways play important roles in linking Myrf deficiency with retinal photoreceptor defects.
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http://dx.doi.org/10.1017/S0952523821000043DOI Listing
May 2021

A multi-magneto-inductive sensor array system for real-time magnetic field imaging of ferromagnetic targets.

Rev Sci Instrum 2021 Mar;92(3):035113

School of Automation, China University of Geosciences, Wuhan, Hubei 430074, China.

In this Note, we develop a real-time magnetic field imaging system by employing a multi-magneto-inductive (MI) sensor array. The sensor array consists of 3 × 3 tri-axial MI sensors, which we constructed by using three sensor coils. Outputs from several rows of sensors are routed to a master-controller responsible for data pre-processing and data reconstruction. The data are streamed to a host computer via a universal serial bus interface, and the image can be generated and displayed at a rate of several frames per second. The magnetic field imaging is implemented on a knowledge of the MI sensors' response, magnetic field perturbations, and the nature of the ferromagnetic object respecting permeability and conductivity. The performance of the system has been further evaluated by extensive numerical modeling of magnetic field distribution patterns with partial differential equation solution. The proposed magnetic field imaging system can be employed in many potential applications, for instance, medicine, security screening, quality assurance, and other areas of nondestructive evaluation, designs associated with magnetic fields, teaching, and research.
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http://dx.doi.org/10.1063/5.0039894DOI Listing
March 2021

Effects of different temperatures on seawater acclimation in rainbow trout Oncorhynchus mykiss: osmoregulation and branchial phospholipid fatty acid composition.

J Comp Physiol B 2021 Jul 5;191(4):669-679. Epub 2021 Apr 5.

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266100, Shandong, China.

This study aimed to investigate the effects of different temperatures on seawater acclimation in rainbow trout (Oncorhynchus mykiss), in terms of growth performance, osmoregulatory capacity, and branchial phospholipid fatty acid (PLFA) composition. The fish (initial weight, 94.73 g) were reared at 9, 12.5, and 16 °C for 28 days, then acclimated to seawater, and subsequently reared for 14 days. Sampling points were on the last day in freshwater, and the 1st, 4th, 7th, and 14th days after the salinity reached 30. The results showed the final weight, percent weight gain, and specific growth rate of rainbow trout at 12.5 °C were significantly higher than those at 9 °C, while the thermal growth coefficient at 16 °C was significantly lower than that in other treatments. The branchial PLFA composition in rainbow trout changed more rapidly at 9 and 12.5 °C than at 16 °C. The branchial PLFA composition was significantly affected by temperature and salinity and their interaction. The polyunsaturated fatty acid content of phospholipids in the gill at 9 and 12.5 °C was significantly higher than those at 16 °C. Low temperature (9 °C) and seawater acclimation significantly increased the degree of unsaturation of membrane, enhancing membrane fluidity, which is related to Na-K ATPase activity. Responses of plasma ion, Na-K ATPase activity, and plasma glucose followed a similar pattern at different temperatures. Overall, the study suggests that 12.5 °C is the ideal temperature for seawater acclimation in rainbow trout.
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http://dx.doi.org/10.1007/s00360-021-01363-zDOI Listing
July 2021

Development and Validation of a Dynamic Nomogram to Predict the Risk of Neonatal White Matter Damage.

Front Hum Neurosci 2020 23;14:584236. Epub 2021 Feb 23.

Neonatal Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Purpose: White matter damage (WMD) was defined as the appearance of rough and uneven echo enhancement in the white matter around the ventricle. The aim of this study was to develop and validate a risk prediction model for neonatal WMD.

Materials And Methods: We collected data for 1,733 infants hospitalized at the Department of Neonatology at The First Affiliated Hospital of Zhengzhou University from 2017 to 2020. Infants were randomly assigned to training ( = 1,216) or validation ( = 517) cohorts at a ratio of 7:3. Multivariate logistic regression and least absolute shrinkage and selection operator (LASSO) regression analyses were used to establish a risk prediction model and web-based risk calculator based on the training cohort data. The predictive accuracy of the model was verified in the validation cohort.

Results: We identified four variables as independent risk factors for brain WMD in neonates by multivariate logistic regression and LASSO analysis, including gestational age, fetal distress, prelabor rupture of membranes, and use of corticosteroids. These were used to establish a risk prediction nomogram and web-based calculator (https://caowenjun.shinyapps.io/dynnomapp/). The C-index of the training and validation sets was 0.898 (95% confidence interval: 0.8745-0.9215) and 0.887 (95% confidence interval: 0.8478-0.9262), respectively. Decision tree analysis showed that the model was highly effective in the threshold range of 1-61%. The sensitivity and specificity of the model were 82.5 and 81.7%, respectively, and the cutoff value was 0.099.

Conclusion: This is the first study describing the use of a nomogram and web-based calculator to predict the risk of WMD in neonates. The web-based calculator increases the applicability of the predictive model and is a convenient tool for doctors at primary hospitals and outpatient clinics, family doctors, and even parents to identify high-risk births early on and implementing appropriate interventions while avoiding excessive treatment of low-risk patients.
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http://dx.doi.org/10.3389/fnhum.2020.584236DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940363PMC
February 2021

Modeling and reduction of the gradient effect of a magnetic sensor for an aeromagnetic compensator.

Rev Sci Instrum 2021 Feb;92(2):024502

Science and Technology on Near-Surface Detection Laboratory, Wuxi 214000, China.

The traditional aeromagnetic compensation model does not consider the negative effects of the gradient of the geomagnetic background field. Especially in emergency aeromagnetic surveys, this gradient effect is obvious and can reduce the performance of magnetic compensation. To solve this problem, we propose a method of reducing the gradient effect of the magnetic sensor for aeromagnetic compensation. A magnetic gradient model of the Earth's background field is established for the aeromagnetic compensation, and the gradient effect is then suppressed using altitude data. An experimental platform was built, and flight tests were conducted. Comparison results show that the adoption of the proposed method increased the improvement ratio (IR) of our compensator on two flights from 20.4 and 21.3 to 25.9 and 26.0, with the growth rates of the IR, respectively, reaching 27% and 22%.
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http://dx.doi.org/10.1063/5.0028025DOI Listing
February 2021

Nanophthalmos-Associated MYRF Gene Mutation Causes Ciliary Zonule Defects in Mice.

Invest Ophthalmol Vis Sci 2021 Mar;62(3)

State Key Laboratory of Ophthalmology, Department of Glaucoma, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China.

Purpose: Patients with nanophthalmos who undergo intraocular surgery often present with abnormal ciliary zonules. In a previous study, we reported mutation in MYRF that is implicated in the pathogenesis of nanophthalmos. The aim of this study was to model the mutation in mice to explore the role of MYRF on zonule structure and its major molecular composition, including FBN1 and FBN2.

Methods: Human MYRF nanophthalmos frameshift mutation was generated in mouse using the CRISPR-Cas9 system. PCR and Sanger sequencing were used for genotype analysis of the mice model. Anterior chamber depth (ACD) was measured using hematoxylin and eosin-stained histology samples. Morphologic analysis of ciliary zonules was carried out using silver staining and immunofluorescence. Transcript and protein expression levels of MYRF, FBN1, and FBN2 in ciliary bodies were quantified using quantitative real-time PCR (qRT-PCR) and Western blot.

Results: A nanophthalmos frameshift mutation (c.789delC, p.N264fs) of MYRF in mice showed ocular phenotypes similar to those reported in patients with nanophthalmos. ACD was reduced in MYRF mutant mice (MYRFmut/+) compared with that in littermate control mice (MYRF+/+). In addition, the morphology of ciliary zonules showed reduced zonular fiber density and detectable structural dehiscence of zonular fibers. Furthermore, qRT-PCR analysis and Western blot showed a significant decrease in mRNA expression levels of MYRF, FBN1, and FBN2 in MYRFmut/+ mice.

Conclusions: Changes in the structure and major molecular composition of ciliary zonules accompanied with shallowing anterior chamber were detected in MYRFmut/+ mice. Therefore, MYRF mutant mice strain is a useful model for exploring pathogenesis of zonulopathy, which is almost elusive for basic researches due to lack of appropriate animal models.
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http://dx.doi.org/10.1167/iovs.62.3.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937999PMC
March 2021

Discovery of a highly potent kinase inhibitor capable of overcoming multiple imatinib-resistant ABL mutants for chronic myeloid leukemia (CML).

Eur J Pharmacol 2021 Apr 11;897:173944. Epub 2021 Feb 11.

Anhui Province Key Laboratory of Medical Physics and Technology; CAS Key Laboratory of High Magnetic Field and Ion Beam Physical Biology; Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, 230031, PR China; University of Science and Technology of China, Hefei, Anhui, 230026, PR China; Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei, Anhui, 230031, PR China; Precision Medicine Research Laboratory of Anhui Province, Hefei, Anhui, 230088, PR China; Institute of Physical Science and Information Technology, Anhui University, Hefei, Anhui, 230601, PR China. Electronic address:

As the critical driving force for chronic myeloid leukemia (CML), BCR gene fused ABL kinase has been extensively explored as a validated target of drug discovery. Although imatinib has achieved tremendous success as the first-line treatment for CML, the long-term application ultimately leads to resistance, primarily via various acquired mutations occurring in the BCR-ABL kinase. Although dasatinib and nilotinib have been approved as second-line therapies that could overcome some of these mutants, the most prevalent gatekeeper T315I mutant remains unconquered. Here, we report a novel type II kinase inhibitor, CHMFL-48, that potently inhibits the wild-type BCR-ABL (wt) kinase as well as a panel of imatinib-resistant mutants, including T315I, F317L, E255K, Y253F, and M351T. CHMFL-48 displayed great inhibitory activity against ABL wt (IC: 1 nM, 70-fold better than imatinib) and the ABL T315I mutant (IC: 0.8 nM, over 10,000-fold better than imatinib) in a biochemical assay and potently blocked the autophosphorylation of BCR-ABL wt and BCR-ABL mutants in a cellular context, which further affected downstream signalling mediators, including signal transducer and activator of transcription 5 (STAT5) and CRK like proto-oncogene (CRKL), and led to the cell cycle progression blockage as well as apoptosis induction. CHMFL-48 also exhibited great anti-leukemic efficacies in vivo in K562 cells and p210-T315I-transformed BaF3 cell-inoculated murine models. This discovery extended the pharmacological diversity of BCR-ABL kinase inhibitors and provided more potential options for anti-CML therapies.
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http://dx.doi.org/10.1016/j.ejphar.2021.173944DOI Listing
April 2021

Screening for upper gastrointestinal cancers with magnetically controlled capsule gastroscopy: a feasibility study.

Endoscopy 2021 09 12;53(9):914-919. Epub 2021 Feb 12.

Department of Gastroenterology, Laboratory of Translational Gastroenterology, and Robot Engineering Laboratory for Precise Diagnosis and Therapy of GI Tumor, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Jinan, Shandong, China.

Background: The medical consortium is an intensive and disease-specific association that integrates tertiary public hospitals and medical examination centers in China. We aimed to evaluate the feasibility of the medical consortium for screening upper gastrointestinal (GI) cancers (MCSC) by magnetically controlled capsule gastroscopy (MCCG).

Methods: 6627 asymptomatic subjects underwent MCCG as part of health check-ups in the MCSC between March and November 2018. Relevant clinical data were collected and analyzed.

Results: The MCSC detected 32 patients with upper GI cancer (0.48 %) confirmed by pathology. The detection rate of early gastric cancer was 16.67 % (4 /24). Gastric polyps, ulcers, and submucosal tumors were found in 15.54 %, 3.76 %, and 3.17 % of subjects, respectively. The whole GI preparation and operation process were well tolerated.

Conclusions: The MCSC was a feasible model for upper GI cancer screening, especially for asymptomatic subjects. Further prospective studies with better operational quality control are warranted.
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http://dx.doi.org/10.1055/a-1333-2120DOI Listing
September 2021

Effects of seawater acclimation at constant and diel cyclic temperatures on growth, osmoregulation and branchial phospholipid fatty acid composition in rainbow trout Oncorhynchus mykiss.

J Comp Physiol B 2021 03 11;191(2):313-325. Epub 2021 Feb 11.

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266100, Shandong Province, China.

The study investigated the effects of seawater acclimation at constant and diel temperatures on the growth, osmoregulation, and branchial phospholipid fatty acid (PLFA) composition in rainbow trout (Oncorhynchus mykiss). The fish (initial weight, 62.28 ± 0.41 g) were reared at a constant 13.0 °C (CT) or with a diel cycle of either 13.0 ± 1.0 °C (VT2) or 13.0 ± 2.0 °C (VT4) for 6 weeks, and subsequently subjected to seawater acclimation. Diel temperature variations (of up to 4 °C) did not affect the growth rate of rainbow trout maintained in freshwater, but alleviated the impairment on the growth after seawater challenge. Under all temperature conditions, rainbow trout were well prepared to seawater acclimation. The diel cyclic temperature resulted in fish with reduced fluctuations in plasma electrolyte levels, branchial Na-K ATPase activity, and plasma osmolality. In freshwater, the sum of the monounsaturated fatty acids was significantly higher in the VT4 relative to CT and VT2 treatment. Conversely, the sum of polyunsaturated fatty acids was significantly lower in the VT4 fish. After seawater transfer, the branchial PLFA profiles of the fish significantly changed, but those in CT and VT2 did not recover afterwards (the degree of unsaturation was downregulated). The PLFA composition of fish in the VT4 treatment appeared to be steadier under seawater acclimation. This study suggests that a diel cyclic temperature (13.0 ± 2.0 °C) can alleviate the impairment of growth, enhance osmoregulation capability, and improve the stability of the branchial PLFA composition in rainbow trout after seawater acclimation.
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http://dx.doi.org/10.1007/s00360-020-01330-0DOI Listing
March 2021

Correlation of Hypoxia-inducible facto-1α and C-reactive protein with disease evaluation in patients with ulcerative colitis.

Am J Transl Res 2020 15;12(12):7826-7835. Epub 2020 Dec 15.

Department of Gastroenterology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University Ji'nan, Shandong Province, China.

Objective: To investigate the expression levels of hypoxia-inducible factor-1α (HIF-1α) and C-reactive protein (CRP) in patients with ulcerative colitis and correlations of HIF-1α and CRP levels with disease severity.

Methods: A total of 82 patients with confirmed ulcerative colitis were enrolled in this study and according to the disease severity grading, these patients were assigned into three groups: mild group (n=25), moderate group (n=31) and severe group (n=26). And other 30 patients without ulcerative colitis as demonstrated by colonoscopy examination were enrolled in control group in the same period. HIF-1α and CRP levels were detected by ELISA and Real-time PCR and compared among different groups. Pearson's correlation analysis was performed to evaluate the correlations of HIF-1α and CRP levels with disease severity. Logistic regression analysis was used to explore risk factors of disease severity in patients with ulcerative colitis.

Results: The expression levels of HIF-1α and CRP in ulcerative colitis group were significantly higher than those in control group (all P<0.001). The levels of HIF-1α and CRP in patients with ulcerative colitis increased remarkably with the increase of disease severity. Patients in mild group had the lowest levels of HIF-1α and CRP, while patients in severe group had the highest levels of HIF-1α and CRP. Logistic regression analysis showed that the expression of HIF-1α and CRP were the risk factors for disease severity of ulcerative colitis (all P<0.001). And Pearson correlation analysis showed that HIF-1α and CRP levels were significantly associated with Rachmilewitz score and disease activity index (DAI), respectively (all P<0.001).

Conclusion: The levels of HIF-1α and CRP were up-regulated in patients with ulcerative colitis and positively correlated with the progression of ulcerative colitis, indicating that the detection of HIF-1α and CRP expression could be used for predicting the disease severity.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791512PMC
December 2020

[Relationship between Circadian Rhythm Disorder of Blood Pressure and Ischemic Stroke].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2020 Dec;42(6):831-835

Department of Neurology,Lianyungang Hospital Affiliated to Xuzhou Medical University,Lianyungang,Jiangsu 222002,China.

Hypertension plays an important role in the pathogenesis of stroke,which,however,is only known at the blood pressure level.The relationship between circadian rhythm of blood pressure(especially the circadian rhythm disorder of blood pressure)and stroke has been a hot research topic.This article reviews the concept of circadian rhythm of blood pressure,classification of circadian rhythm disorder of blood pressure,and the relationship of circadian rhythm disorder of blood pressure with ischemic stroke.
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http://dx.doi.org/10.3881/j.issn.1000-503X.12152DOI Listing
December 2020

Exosomes derived from retinoblastoma cells enhance tumour deterioration by infiltrating the microenvironment.

Oncol Rep 2021 01 18;45(1):278-290. Epub 2020 Nov 18.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑sen University, Guangzhou, Guangdong 510000, P.R. China.

The survival of young children (under 5 years of age) with malignant retinoblastoma remains poor, and clarification of the mechanism underlying tumour development is urgently needed. The present study aimed to reveal the role of exosomes (EXOs) from retinoblastoma cells in tumour development. The in vitro data indicated that EXOs derived from WERI‑Rb1 cells significantly inhibited the antitumour activity of macrophages and induced bone marrow mesenchymal stem cells to promote tumour growth via an increase in monocyte chemotactic protein 1 (also known as C‑C motif chemokine ligand 2) levels. In vivo data from a xenotransplantation model also showed that EXOs infiltrated the spleen, which induced a decrease in leukocytes and natural killer (NK) cells. Accordingly, the proportion of tumour‑associated macrophages was increased and the proportion of NK cells was decreased in tumours injected with EXOs compared with those injected with the control. EXOs were absorbed by Kupffer cells, and more metastases were observed in the liver. Thus, these results suggested that EXOs derived from retinoblastoma promoted tumour progression by infiltrating the microenvironment. Moreover, microRNAs (miRs), including miR‑92a, miR‑20a, miR‑129a and miR‑17, and C‑X‑C chemokine receptor type 4 and thrombospondin‑1 were detectable in EXOs, which might account for EXO‑mediated tumour deterioration.
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http://dx.doi.org/10.3892/or.2020.7858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7716702PMC
January 2021

Clinical and imaging features of reversible splenial lesion syndrome with language disorder.

Transl Neurosci 2020 19;11(1):210-214. Epub 2020 Jun 19.

Department of Neurology, The affiliated Lianyungang Hospital of Xuzhou Medical University, Tongguan North Road, No. 182, Haizhou District, Lianyungang, Jiangsu, China.

Reversible splenial lesion syndrome (RESLES) is a single-stage non-specific syndrome with unclear pathogenesis. There has been no report on answer delay in patients with RESLES. We report a female patient who was admitted to our department for mixed aphasia accompanied by cognitive impairment. During the rapid improvement of aphasia, there was a clear phase of language output response delay accompanied by resolution of imaging lesions. We analyzed the course and the examination results of the patient and speculated the cause and pathogenesis. RESLES-relevant knowledge was systematically reviewed, which will help doctors in the classification of cerebral function and the diagnosis of RESLES. The specific language and cognitive impairment may be associated with the damage of contact fibers in the bilateral primary and secondary sensory and motor cortices.
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http://dx.doi.org/10.1515/tnsci-2020-0126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712031PMC
June 2020

miR‑181d promotes cell proliferation via the IGF1/PI3K/AKT axis in glioma.

Mol Med Rep 2020 Nov 26;22(5):3804-3812. Epub 2020 Aug 26.

Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

Glioma is a malignant brain cancer that exhibits high invasive ability and poor prognosis. MicroRNA (miR)‑181d has been reported to be involved in the development of glioma. Therefore, the aim of the present study was to investigate whether miR‑181d affected cellular progression by influencing the insulin like growth factor (IGF1)/PI3K/AKT axis. Western blot analysis was performed to analyze the expression levels of specific proteins, and a Cell Counting Kit‑8 assay was used to assess the proliferative ability of cells. Cell cycle progression and cellular apoptosis were both measured using flow cytometry. The results indicated that miR‑181d promoted cellular proliferation and cell cycle progression, while suppressing cellular apoptosis via the IGF1/PI3K/AKT axis. It was demonstrated that the IGF1 and PI3K/AKT inhibitors reversed these observed functions of miR‑181d. Furthermore, miR‑181d enhanced the growth of glioma xenografts in vivo, promoted cell cycle progression and suppressed cellular apoptosis within glioma xenograft tissues. Therefore, this newly identified miR‑181d/IGF1/PI3K/AKT axis may provide novel insights into the pathogenesis of glioma.
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http://dx.doi.org/10.3892/mmr.2020.11464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533453PMC
November 2020

Age and Diastolic Blood Pressure Play an Important Role in the Progression of White Matter Lesions: A Meta-Analysis.

Eur Neurol 2020 9;83(4):351-359. Epub 2020 Sep 9.

Department of Neurology, The affiliated Lianyungang Hospital of Xuzhou Medical University, Lianyungang, China,

Introduction: White matter lesions (WMLs) are currently considered as a cerebral microvascular disease, and hypertension is considered as its most important risk factor. This study analysis systematically evaluated the effects of systolic blood pressure (SBP) and diastolic blood pressure (DBP) on the progression of WMLs.

Methods: We searched the MeSH terms: "white matter," "blood pressure," "hypertension," "Leukoencephalopathy," and "leukoaraiosis" on PubMed and Cochrane from January 2000 to August 2019. A total of 12 closely related literature were included for research.

Results: The results of the meta-analysis showed that the increase of both SBP and DBP can promote the progression of WMLs (respectively, odds ratio [OR] = 2.90, 95% confidence interval [CI] 2.86-2.94; OR = 3.13, 95% CI 3.03-3.23). Subgroup analysis found that patients with hypertension aged younger than 70 years are at a greater risk of WML progression when their DBP increased (OR = 4.69, 95% CI 3.31-6.65).

Conclusion: The relationship between DBP and WMLs is closer than that of SBP. Also, the risk of WML progression in patients aged under 70 years was higher than that in patients aged over 70 years. Furthermore, when DBP is elevated in patients younger than 70 years of age, the risk of WML progression may be higher. Therefore, it is expected that more researchers will attach importance to the change in DBP and identify the range of blood pressure and strategies that control DBP, thus contributing to delay the progression of WMLs.
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http://dx.doi.org/10.1159/000510077DOI Listing
February 2021

Compressed sensing based tuning algorithm for the sensor of proton precession magnetometers.

Rev Sci Instrum 2020 Aug;91(8):085118

Science and Technology on Near-Surface Detection Laboratory, Wuxi, Jiangsu 214035, China.

In this Note, a new compressed sensing-based tuning algorithm has been developed to boost the sensor tuning performance of the proton precession magnetometers (PPMs). An end-to-end framework for the PPM's sensing free induction decay (FID) signal resonance based on orthogonal matching pursuit compressed sensing (OMPCS), dubbed OMPCS-FID resonance (OMPCS-FIDR), is developed and its working principle and implemented strategy are elaborated. By comparing the new sensor tuning approach with the state-of-the-art algorithms, i.e., peak detection, auto-correction, and secondary tuning, the results demonstrate that the proposed tuning method not only retains the performance but also overcomes the drawbacks of the state-of-the-art methods, which accelerates the possibilities of the PPM working in a scenario with a strong gradient magnetic field.
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http://dx.doi.org/10.1063/5.0015350DOI Listing
August 2020

miR-34a carried by adipocyte exosomes inhibits the polarization of M1 macrophages in mouse osteolysis model.

J Biomed Mater Res A 2021 06 13;109(6):994-1003. Epub 2020 Sep 13.

Department of Orthopedics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.

Objective: After bone prosthesis replacement, M1-type macrophage polarization can be induced by titanium (Ti) particles and produce inflammatory, leading to osteolysis. Adipocyte-derived exosomes (ADEs) exert immune-modulatory impact on the macrophage, while whether it can inhibit the macrophage polarization induced by Ti is unclear. This study focuses on the M1-type macrophage and aims to determine the effect of ADEs on Ti-induced M1-type macrophage polarization in osteolytic mice and the involved mechanism.

Methods: Ti particle-induced osteolysis mouse model was established and macrophages were isolated from the osteolysis site. The levels of NLRP3 and specific markers for M1-type macrophage were determined. ADEs isolated from adipocyte cell line 3T3-L1, or conditioned ADEs with low-expressed miR-34a isolated from 3T3-L1 transfected with miR-34a inhibitor were co-cultured with RAW 264.7 to determine their impact on the polarization of macrophage.

Results: ADEs reduced the M1-type macrophage polarization and caused the upregulation of miR-34a in macrophage of the osteolysis site of the osteolysis mouse model. Also, the level of miR-34a in ADEs was higher than that in the adipocyte. The conditioned ADEs expressed a low level of miR-34a and boosted the Ti-induced M1-type polarization. MiR-34a could target NLRP3 and negatively regulated its expression. Moreover, NLRP3 knockdown in macrophage restricted the conditioned ADEs to promote macrophage towards to Ti-induced M1-type polarization. The inhibitory function of ADEs on M1-type macrophage polarization was abolished by miR-34a silencing in the mouse osteolysis model.

Conclusion: The miR-34a carried by ADEs reduced the polarization of M1-type macrophages by targeting macrophage NLRP3 during Ti particle-induced osteolysis.
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http://dx.doi.org/10.1002/jbm.a.37088DOI Listing
June 2021
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