Publications by authors named "Jian Gao"

1,043 Publications

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Novel association between a TRPM5 expression quantitative trait locus rs35197079 and decreased susceptibility of gestational diabetes mellitus in a Chinese population.

J Diabetes Investig 2021 May 12. Epub 2021 May 12.

Shenzhen Maternity and Child Healthcare Hospital, Futian District, 4009 Xinzhou Road, Shenzhen, China.

Purpose: Emerging evidence suggests that expression quantitative trait loci (eQTLs) are more likely to associate with complex diseases. TRPM5 is an ubiquitously expressed voltage-gated cation channel which acts indispensably to trigger insulin secretion in pancreatic β cells. This study evaluated the association between TRPM5 eQTL SNPs and the risk of gestational diabetes mellitus (GDM) in a Chinese population.

Patients And Methods: A total of 380 unrelated Chinese pregnant women including 241 GDM patients and 139 controls were included in this study. The eQTL SNPs of TRPM5 were obtained from GTEx eQTL Browser, and were subsequently genotyped using the Agena MassARRAY iPLEX platform.

Results: Logistic regression analysis and linear regression analysis showed that rs35197079 and rs74848824 were significantly associated with reduced GDM risk and lower fasting plasma glucose levels after adjusting confounder factors in dominant genetic models. Stratification analysis based on pre-pregnancy BMI validated strong association between rs35197079 and GDM susceptibility in underweight and normal weight subjects. Luciferase and electrophoretic mobility shift assays conducted in rat pancreatic β cells showed that rs35197079 was functional.

Conclusion: The TRPM5 eQTL SNP rs35197079 was associated with decreased GDM susceptibility in a Chinese population, especially in underweight and normal weight pregnant women, and it was functional in modulating gene transcription.
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http://dx.doi.org/10.1111/jdi.13572DOI Listing
May 2021

A critical review on the distribution and ecological risk assessment of steroid hormones in the environment in China.

Sci Total Environ 2021 Apr 30;786:147452. Epub 2021 Apr 30.

Chair of Ecological Systems Design, Institute of Environmental Engineering, ETH Zürich, 8093 Zürich, Switzerland.

During past two decades, steroid hormones have raised significant public concerns due to their potential adverse effects on the hormonal functions of aquatic organisms and humans. Considering China being a big producer and consumer of steroid hormones, we summarize the current contamination status of steroid hormones in different environmental compartments in China, and preliminarily assess the associated risks to ecological systems. The results show that steroid hormones are ubiquitously present in Chinese surface waters where estrogens are the most studied steroids compared with androgens, progestogens and glucocorticoids. Estrone (E1), 17β-estradiol (17β-E2) and estriol (E3) are generally the dominant steroid estrogens in Chinese surface waters, whereas for the other steroids, androsterone (ADR), epi-androsterone (EADR), progesterone (PGT), cortisol (CRL) and cortisone (CRN) have relatively large contributions. Meanwhile, the investigations for the other environmental media such as particles, sediments, soils and groundwater have been limited, as well as for steroid conjugates and metabolites. The median risk quotients of most steroid hormones in surface waters and sediments are lower than 1, indicating low to moderate risks to local organisms. This review provides a full picture of steroid distribution and ecological risks in China, which may be useful for future monitoring and risk assessment. More studies may focus on the analysis of steroid conjugates, metabolites, solid phase fractions, analytical method development and acute/chronic toxicities in different matrices to pursue a more precise and holistic risk assessment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147452DOI Listing
April 2021

Atroposelective Synthesis of Axially Chiral 4-Aryl α-Carbolines via -Heterocyclic Carbene Catalysis.

Org Lett 2021 May 11. Epub 2021 May 11.

State Key Laboratory of Natural Medicines, Department of Organic Chemistry, China Pharmaceutical University, Nanjing 210009, P. R. China.

The first catalytic asymmetric construction of axially chiral 4-aryl α-carboline skeletons has been accomplished through an -heterocyclic carbene (NHC)-catalyzed atroposelective formal [3 + 3] annulation of 4-nitrophenyl 3-arylpropiolates with 2-sulfonamidoindolines. The synthetic utility of the title compounds has been demonstrated by the diverse late-stage structural modifications. Density functional theory calculations were also conducted to illuminate the key factors for controlling the origin of the enantioselectivity. This strategy not only provides an efficient pathway to access axially chiral α-carboline atropisomers but also offers a novel catalytic enantioselective mode for the construction of axially chiral heterobiaryls by using NHC-bound alkynyl acylazoliums.
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http://dx.doi.org/10.1021/acs.orglett.1c01221DOI Listing
May 2021

A Novel Clinical Nomogram for Predicting Cancer-Specific Survival in Adult Patients After Primary Surgery for Epithelial Ovarian Cancer: A Real-World Analysis Based on the Surveillance, Epidemiology, and End Results Database and External Validation in a Tertiary Center.

Front Oncol 2021 20;11:670644. Epub 2021 Apr 20.

Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, China.

Background: The present study aimed to construct and validate a nomogram that can be used to predict cancer-specific survival (CSS) in patients with epithelial ovarian cancer (EOC).

Methods: A total of 7,129 adult patients with EOC were extracted from the Surveillance, Epidemiology, and End Results database between 2010 and 2015. Patients were randomly divided into the training and validation cohorts (7:3). Cox regression was conducted to evaluate prognostic factors of CSS. The internal validation of the nomogram was performed using concordance index (C-index), AUC, calibration curves, and decision curve analyses (DCAs). Data from 53 adult EOC patients at Shengjing Hospital of China Medical University from 2008 to 2012 were collected for external verification. Kaplan-Meier curves were plotted to compare survival outcomes among risk subgroups.

Results: Age, grade, histological types, stage, residual lesion size, number of regional lymph nodes resected, number of positive lymph nodes, and chemotherapy were independent risk factors for CSS. Based on the above factors, we constructed a nomogram. The C-indices of the training cohort, internal validation cohort, and external verification group were 0.763, 0.750, and 0.920, respectively. The calibration curve indicated good agreement between the nomogram prediction and actual survival. AUC and DCA results indicated great clinical usefulness of the nomogram. The differences in the Kaplan-Meier curves among different risk subgroups were statistically significant.

Conclusions: We constructed a nomogram to predict CSS in adult patients with EOC after primary surgery, which can assist in counseling and guiding treatment decision making.
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http://dx.doi.org/10.3389/fonc.2021.670644DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093627PMC
April 2021

Anchoring Ir(iii) complex on macroscopic polymer substrate as highly durable photosensitizer for photocatalytic hydrogen evolution.

Chem Commun (Camb) 2021 Apr 26;57(32):3857-3860. Epub 2021 Mar 26.

CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. and University of Science and Technology of China, Hefei, 230026, China.

A novel photosensitizer (PS) was developed by anchoring an Ir(iii) complex on a macroscopic polymer substrate to prevent ligand dissociation of the PS, resulting in a long duration of over 730 h in photocatalytic hydrogen evolution. This study paves the way for designing new Ir(iii) PSs and exploring the inactivation mechanism of PSs.
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http://dx.doi.org/10.1039/d1cc00083gDOI Listing
April 2021

Transcriptomic dynamics changes related to anthocyanin accumulation in the fleshy roots of carmine radish ( L.) characterized using RNA-Seq.

PeerJ 2021 7;9:e10978. Epub 2021 Apr 7.

Research Center for Tourism Agriculture Development, Sichuan Tourism College, Chengdu, China.

Carmine radish is famous for containing a natural red pigment (red radish pigment). However, the expression of anthocyanin biosynthesis-related genes during the dynamic development stages of the fleshy roots in carmine radish has not been fully investigated. Here, based on HPLC quantification of anthocyanin levels from our previous study, young fleshy roots of the carmine radish "Hongxin 1" obtained at the dynamic development stages of fleshy roots (seedling stage (SS), initial expansion (IE), full expansion (FE), bolting stage (BS), initial flowering stage (IFS), full bloom stage (FBS) and podding stage (PS)) were used for RNA-Seq. Approximately 126 comodulated DEGs related to anthocyanin biosynthesis (common DEGs in the dynamic growth stages of fleshy roots in carmine radish) were identified, from which most DEGs appeared to be likely to participate in anthocyanin biosynthesis, including two transcription factors, and . In addition, some related proteins, e.g., and , were found as candidate contributors to the regulatory mechanism of anthocyanin synthesis in the fleshy roots of carmine radish. In addition, 11 putative DEGs related to anthocyanin synthesis were evaluated by qRT-PCR via the (2-ΔΔCT) method; the Pearson correlation analysis indicated excellent concordance between the RNA-Seq and qRT-PCR results. Furthermore, GO enrichment analysis showed that "anthocyanin-containing compound biosynthetic process" and "anthocyanin-containing compound metabolic process" were commonly overrepresented in the dynamic growth stages of fleshy roots after the initial expansion stage. Moreover, five significantly enriched pathways were identified among the DEGs in the dynamic growth stages of fleshy roots in carmine radish, namely, flavonoid biosynthesis, flavone and flavonol biosynthesis, diterpenoid biosynthesis, anthocyanin biosynthesis, and benzoxazinoid biosynthesis. In conclusion, these results will expand our understanding of the complex molecular mechanisms of anthocyanin biosynthesis in the fleshy roots of carmine radish and the putative candidate genes involved in this process.
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http://dx.doi.org/10.7717/peerj.10978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035900PMC
April 2021

Optimal pH shift of the NADH oxidase from Lactobacillus rhamnosus with a single mutation.

Biotechnol Lett 2021 Apr 12. Epub 2021 Apr 12.

School of Pharmacy, Jiangsu University, Zhenjiang, 212013, People's Republic of China.

Objective: To improve the activity of a water-forming NADH oxidase from Lactobacillus rhamnosus under neutral or alkaline pH for coupling NAD-dependent dehydrogenases with an alkaline optimal pH.

Results: The water-forming NADH oxidase from Lactobacillus rhamnosus was engineered by replacing the aspartic acid or glutamic acid with arginine on the surface. The mutant D251R improved the activity with a 112%, 111%, and 244% relative activity to the wild-type at pH 6.5, pH 7.0, and pH 7.5, respectively. Docking substrate into the D251R mutant reveals that the NADH is access to the substrate-binding site with a larger substrate loop due to the enhanced electrostatic repulsion between ARG-251 and ARG-243. In the D251R-NADH complex, the carboxyl of NADH additionally forms two hydrogen bonds (2.6 and 2.9 Å) with G154 due to the changed interaction of substrate and the residues in the catalytic sites, and the hydrogen bond with the oxygen of carbonyl in P295 is shortened from 2.9 to 2.0 Å, which could account for the enhanced specific activity.

Conclusions: The D251R mutant displayed higher catalytic activity than the wild-type in the pH range 6.5-7.5, and further insight into those shorter and newly formed hydrogen bonds in substrate docking analysis could account for the higher bind affinity and catalytic efficiency of D251R mutant.
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http://dx.doi.org/10.1007/s10529-021-03129-7DOI Listing
April 2021

Serum creatinine as a predictor of mortality in patients readmitted to the intensive care unit after cardiac surgery: a retrospective cohort study in China.

J Thorac Dis 2021 Mar;13(3):1728-1736

Department of Nursing, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Patients readmitted to the intensive care unit (ICU) after cardiac surgery have a high mortality rate. The relationship between renal function and in-hospital mortality in readmitted patients has not been well demonstrated.

Methods: We retrospectively evaluated cardiac surgery patients who were readmitted to the ICU at least once. Data on serum creatinine levels before surgery and on the day of ICU readmission were collected. The estimated glomerular filtration rate (eGFR) was calculated according to the creatinine-based Chronic Kidney Disease-Epidemiology Collaboration equation. We used logistic regression models and restricted cubic spline curves with four knots (5%, 35%, 65%, 95%) to investigate the relationship between renal function indicators and mortality.

Results: Of the 184 patients evaluated, 30 patients died during hospitalization, yielding a mortality rate of 16.30%. Cardiac dysfunction (n=84, 45.65%) and respiration disorder (n=51, 27.72%) were the most common reasons for ICU readmission. Creatinine [odds ratio (OR): 1.14, 95% confidence interval (CI): 1.07-1.25] and eGFR (OR: 0.95, 95% CI: 0.93-0.98) were independently associated with in-hospital mortality after adjusting for various confounders. Both creatinine level and eGFR had a linear association with in-hospital mortality (P for non-linearity ˃0.05).

Conclusion: Renal function is significantly associated with the in-hospital mortality of patients readmitted to the ICU after cardiac surgery, as evidenced by the independent correlation of both creatinine and eGFR with in-hospital mortality.
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http://dx.doi.org/10.21037/jtd-20-3205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024843PMC
March 2021

Potential targets identified in adenoid cystic carcinoma point out new directions for further research.

Am J Transl Res 2021 15;13(3):1085-1108. Epub 2021 Mar 15.

Department of Oral and Maxillofacial Surgery, Jinan Stomatological Hospital Jinan, China.

Adenoid cystic carcinoma (AdCC) of the head and neck originates from salivary glands, with high risks of recurrence and metastasis that account for the poor prognosis of patients. The purpose of this research was to identify key genes related to AdCC for further investigation of their diagnostic and prognostic significance. In our study, the AdCC sample datasets GSE36820, GSE59702 and GSE88804 from the Gene Expression Omnibus (GEO) database were used to explore the abnormal coexpression of genes in AdCC compared with their expression in normal tissue. A total of 115 DEGs were obtained by screening with GEO2R and FunRich software. According to functional annotation analysis using Enrichr, these DEGs were mainly enriched in the SOX2, AR, SMAD and MAPK signaling pathways. A protein-protein network of the DEGs was established by the Search Tool for the Retrieval of Interacting Genes (STRING) and annotated through the WEB-based Gene SeT AnaLysis Toolkit (WebGestalt) and was shown to be enriched with proteins involved in cardiac muscle cell proliferation and extracellular matrix organization. A Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that ITGA9, LAMB1 and BAMBI were associated with the PI3K-Akt and TGF-β pathways. Furthermore, 36 potential target miRNAs were identified by the OncomiR and miRNA Pathway Dictionary Database (miRPathDB). In conclusion, SLC22A3, FOXP2, Cdc42EP3, COL27A1, DUSP1 and HSPB8 played critical roles according to the enrichment analysis; ITGA9, LAMB1 and BAMBI were involved in significant pathways according to the KEGG analysis; ST3Gal4 is a pivotal component of the PPI network of all the DEGs obtained; SPARC, COL4A2 and PRELP were highly related to multiple malignancies in pan-cancer research; hsa-miR-29-3p, hsa-miR-132-3p and hsa-miR-708-5p were potential regulators in AdCC. The involved pathways, biological processes and miRNAs have been shown to play significant roles in the genesis, growth, invasion and metastasis of AdCC. In this study, these identified DEGs were considered to have a potential influence on AdCC but have not been studied in this disease. The analysis results promote our understanding of the molecular mechanisms and biological processes of AdCC, which might be useful for targeted therapy or diagnosis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014416PMC
March 2021

Factors influencing the short-term and long-term survival of hepatocellular carcinoma patients with portal vein tumor thrombosis who underwent chemoembolization.

Authors:
Ke-Li Chen Jian Gao

World J Gastroenterol 2021 Apr;27(13):1330-1340

Department of Gastroenterology and Hepatology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.

Background: The factors affecting the short-term and long-term prognosis of hepatocellular carcinoma (HCC) patients with portal vein tumor thrombosis (PVTT) receiving transarterial chemoembolization (TACE) are still unclear.

Aim: To clarify the predictors correlated with the short-term and long-term survival of HCC patients with PVTT who underwent TACE.

Methods: The medical records of 181 HCC patients with PVTT who underwent TACE at the Second Affiliated Hospital of Chongqing Medical University from January 2015 to July 2019 were retrospectively analyzed. We explored the short-term and long-term prognostic factors by comparing the preoperative indicators of patients who died and survived within 3 mo and 12 mo after TACE. Multivariate analyses were conducted using logistic regression. The area under the receiver operating characteristic curve (area under curve) was used to evaluate the predictive ability of the factors related to the short-term and long-term prognosis.

Results: The median survival time was 4.8 mo (range: 2.5-8.85 mo). The 3 mo, 6 mo, and 12 mo survival rates were 68.5%, 38.7%, and 15.5%, respectively. In multivariable analysis, total bilirubin, sex, and aspartate aminotransferase (AST) were closely linked to short-term survival. When AST ≥ 87 U/L and total bilirubin ≥ 16.15 µmol/L, the 3-mo survival rate after TACE was reduced significantly ( < 0.05). AST had the best predictive ability, followed by total bilirubin, while sex had the worst predictive ability for short-term survival area under curve: 0.763 (AST) 0.707 (total bilirubin) 0.554 (sex)]. The long-term survival outcome was significantly better in patients with a single lesion than in those with ≥ three lesions ( = 0.009). Patients with massive block HCC had a worse long-term survival than patients with nodular and diffuse HCC ( = 0.001).

Conclusion: AST, total bilirubin, and sex are independent factors associated with short-term survival. The number of tumors and the gross pathological type of tumor are related to the long-term outcome.
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http://dx.doi.org/10.3748/wjg.v27.i13.1330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015298PMC
April 2021

Metabolic Abnormalities in Patients with Chronic Disorders of Consciousness.

Aging Dis 2021 Apr 1;12(2):386-403. Epub 2021 Apr 1.

1Department of Neurology and Brain Medical Center, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.

The vegetative state (VS) and minimally conscious state (MCS) are two major types of chronic disorders of consciousness (DoC). The assessment of these two consciousness states generally relies on the Coma Recovery Scale-Revised (CRS-R) score, but a high misdiagnosis rate limits the generalized use of this score. To identify metabolites in human plasma that can accurately distinguish VS from MCS patients, comprehensive plasma metabolic profiles were obtained with targeted metabolomics analysis and untargeted and targeted lipidomics analysis. Univariate and multivariate analyses were used to assess the significance of differences. Compared with healthy controls (HCs), the DoC groups, Emerged from Minimally Conscious State (EMCS) group and Alzheimer's disease (AD) group had significantly different metabolic profiles. Purine metabolism pathway differed the most between the DoC (MCS and VS) and HC groups. In this pathway, adenosine, ADP, and AMP, which are the derived products of ATP degradation, were decreased in the MCS and VS groups compared to healthy controls. More importantly, we identified certain lipids for which the levels were enriched in the VS or MCS groups. Specifically, phosphatidylcholine, (38:5)-H (PC(38:5)-H), and arachidonic acid (AA) differed substantially between the VS and MCS groups and may be used to distinguish these two groups of patients. Together, our findings suggest that metabolic profiling is significantly altered in patients with chronic DoC.
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http://dx.doi.org/10.14336/AD.2020.0812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990357PMC
April 2021

Zanthoxylum bungeanum Seed Oil Elicits Autophagy and Apoptosis in Human Laryngeal Tumor Cells via PI3K/AKT/mTOR Signaling Pathway.

Anticancer Agents Med Chem 2021 Mar 31. Epub 2021 Mar 31.

School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, Shanxi Province. China.

Background: Zanthoxylum bungeanum seed oil (ZBSO) is a main extract of the edible drug Zanthoxylum bungeanum seeds. Recently reports proved that it has a significant cytotoxic effect on various cancer cells. However, systematic investigation on the roles of ZBSO in laryngeal carcinoma (LC) is rare.

Objective: To reveal the function of ZBSO on human laryngeal squamous carcinoma cells (Hep-2) and to elucidate its underlying mechanism.

Methods: In this study, the chemical composition analysis of ZBSO was done using Ultra Performance Liquid Chromatography (UPLC), and the anti-tumor effect of ZBSO on Hep-2 cells was evaluated by cell proliferation, apoptosis and cell cycle experiments. qRT-PCR, immunohistochemistry (IHC) and Western blotting were used for mechanistic investigation at the molecular level.

Results: The main compound of ZBSO was identified as polyunsaturated fatty acids. Furthermore, as compared with normal cells, significant inhibitory activities of ZBSO was observed on Hep-2 cells with dose- and time-dependency, which induced apoptosis, blocked cell cycle at the S phase, and inhibited cell proliferation. In addition, IHC results showed difference in the level of protein expression of ZBSO-induced autophagy-related markers. At last, Western blotting results indicated that ZBSO could inhibit the expression and phosphorylation levels of PI3K/AKT/mTOR protein.

Conclusions: The anti-LC effect of ZBSO might be intimately associated with the induction of autophagy and the inhibition of PI3K/AKT/mTOR signaling pathway. ZBSO may be a potential anti-laryngocarcinoma agent.
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http://dx.doi.org/10.2174/1871520621666210401103820DOI Listing
March 2021

Single-cell analysis reveals innate immunity dynamics in ankylosing spondylitis.

Clin Transl Med 2021 Mar;11(3):e369

State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, and Human Phenome Institute, Fudan University, Shanghai, China.

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http://dx.doi.org/10.1002/ctm2.369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982614PMC
March 2021

Streptomyces liangshanensis sp. nov., a novel actinomycete isolated from rhizosphere soil of Fagopyrum tataricum.

Arch Microbiol 2021 Mar 30. Epub 2021 Mar 30.

School of Life Science, Hunan University of Science and Technology, Xiangtan, 411201, People's Republic of China.

A novel actinomycete strain, designated strain QMT-12, was isolated from the rhizospheric soils of Fagopyrum tataricum and characterized using a polyphasic approach. Strain QMT-12 was found to have morphological features typical of the genus Streptomyces. The predominant fatty acids included C cis9 (35.9%), Summed feature 6 (C cis9, 12/C a or C anteiso/C c) (30.6%) and C (16.3%). The whole-cell sugars were arabinose and glucose. The whole-cell-wall amino acids included alanine, aspartate, glutamic acid, glycine and LL-diaminopimelic acid. The menaquinones were MK-9, MK-9(H), MK-9(H), MK-9(H) and MK-9(H). The diagnostic phospholipids consisted of diphosphatidyl glycerol, phosphatidylethanolamine, phosphatidyl methyl ethanolamine, phospholipids, phosphotidyl inositol, phosphotidylinositol mannosides, and phospholipids of unknown structure containing glucosamine. The full-length 16S rRNA gene sequence analysis showed that strain QMT-12 belonged to the genus Streptomyces and had 98.2, 98.1, 98.1 and ≤ 98.0% similarities to Streptomyces camponoticapitis 2H-TWYE14, Streptomyces scopuliridis NRRL B-24574, Streptomyces inhibens NEAU-D10 and other Streptomyces species with validly published and correct names, respectively. Phylogenetic analysis indicated that strain QMT-12 was closely related to Streptomyces inhibens NEAU-D10. However, the average nucleotide identity value and the digital DNA-DNA hybridization value between strain QMT-12 and S. inhibens NEAU-D10 were 85.0 and 22.3%, respectively, well below 95-96% and 70% cut-off point recommended for delineating species. Based on its phenotypic and genotypic characteristics, strain QMT-12 (= CICC 11056 = JCM 33963) represents the type strain of a novel species, for which the name Streptomyces liangshanensis sp. nov. is proposed.
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http://dx.doi.org/10.1007/s00203-021-02294-7DOI Listing
March 2021

Genome-wide identification and functional characterization of the PheE2F/DP gene family in Moso bamboo.

BMC Plant Biol 2021 Mar 29;21(1):158. Epub 2021 Mar 29.

International Center for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and Technology, State Forestry Administration, Beijing, China.

Background: E2F/DP proteins have been shown to regulate genes implicated in cell cycle control and DNA repair. However, to date, research into the potential role of the Moso bamboo E2F/DP family has been limited.

Results: Here, we identified 23 E2F/DPs in the Moso bamboo genome, including nine E2F genes, six DP genes, eight DEL genes and one gene with a partial E2F domain. An estimation of the divergence time of the paralogous gene pairs suggested that the E2F/DP family expansion primarily occurred through a whole-genome duplication event. A regulatory element and coexpression network analysis indicated that E2F/DP regulated the expression of cell cycle-related genes. A yeast two-hybrid assay and expression analysis based on transcriptome data and in situ hybridization indicated that the PheE2F-PheDP complex played important roles in winter Moso bamboo shoot growth. The qRT-PCR results showed that the PheE2F/DPs exhibited diverse expression patterns in response to drought and salt treatment and diurnal cycles.

Conclusion: Our findings provide novel insights into the Moso bamboo E2F/DP family and partial experimental evidence for further functional verification of the PheE2F/DPs.
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http://dx.doi.org/10.1186/s12870-021-02924-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008544PMC
March 2021

E3 Ubiquitin Ligase UBR5 Promotes the Metastasis of Pancreatic Cancer Destabilizing F-Actin Capping Protein CAPZA1.

Front Oncol 2021 12;11:634167. Epub 2021 Mar 12.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The ubiquitin-proteasome system (UPS) is a regulated mechanism of intracellular protein degradation and turnover, and its dysfunction is associated with various diseases including cancer. UBR5, an E3 ubiquitin ligase, is emerging as an important regulator of the UPS in cancers, but its role in pancreatic cancer is poorly understood. Here, we show that UBR5 is significantly upregulated in pancreatic cancer tissues. High UBR5 expression is correlated with increased lymph node metastasis and poor survival of patients. The loss-of-function and gain-of-function studies demonstrated that UBR5 substantially enhanced the migratory and invasive ability of pancreatic cancer cells. UBR5 knockdown also markedly inhibited cancer metastasis in the liver metastatic model of pancreatic cancer in nude mice, suggesting UBR5 as a potent metastatic promoter in pancreatic cancer. Furthermore, using co-immunoprecipitation combined with mass spectrometry analyses, CAPZA1, a member of F-actin capping protein α subunit family, was identified as a novel substrate of UBR5. UBR5 overexpression could promote the degradation of CAPZA1 via the UPS and induce the accumulation of F-actin, which has been described as an essential molecular event during the process of CAPZA1 deficiency-induced cancer cells migration and invasion. UBR5 knockdown significantly increased the intracellular level of CAPZA1 and CAPZA1 downregulation largely reversed the UBR5 knockdown-induced suppression of cell migration and invasion in pancreatic cancer cells. Collectively, our findings unveil UBR5 as a novel and critical regulator of pancreatic cancer metastasis and highlight the potential for UBR5-CAPZA1 axis as a therapeutic target for preventing metastasis in pancreatic cancer patients, especially in those with increased UBR5 expression.
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http://dx.doi.org/10.3389/fonc.2021.634167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994773PMC
March 2021

Comparative Study of Cytotoxicity, DNA Damage and Oxidative Stress Induced by Heavy Metals Cd(II), Hg(II) and Cr(III) in Yeast.

Curr Microbiol 2021 May 26;78(5):1856-1863. Epub 2021 Mar 26.

Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, Canterbury, 7647, New Zealand.

Wide range of applications of heavy metals and improperly discarded their castoffs possess serious threats to environment and human health. In this study, cytotoxicity, DNA damage and oxidative stress induced by Cd(II), Hg(II) and Cr(III) were comparatively studied in yeast Saccharomyces cerevisiae. Cd(II), Hg(II), and Cr(III) all produced strong cytotoxicity resulting in growth inhibition and cell mortality to varying degrees (Hg(II) > Cd(II) > Cr(III)). Hg(II) produced more oxidative stress. Cr(III) caused more serious DNA damage in vitro. Cd(II) also caused both obvious DNA damage and oxidative stress at higher concentration, but not as efficiently as Cd(II) and Hg(II). A further null mutation sensitivity assay showed that the relative sensitivity of rad1∆ to the metals was Cr(III) > Cd(II) > Hg(II), and that of trx1∆ to the metals was Hg(II) > Cd(II) > Cr(III). These data provide a clear evidence that the Cr(III) can cause significant DNA damage and potential genotoxicity; Hg(II) can strongly inhibit SOD activity, produce lipid peroxidation and cause serious membrane injury, suggesting these heavy metals can cause different toxic effects in different ways.
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http://dx.doi.org/10.1007/s00284-021-02454-4DOI Listing
May 2021

Does the Lingual-Based Mucoperiosteal Flap Reduce Postoperative Morbidity Compared With the Buccal-Based Mucoperiosteal Flap After the Surgical Removal of Impacted Third Molars? A Meta-analysis Review.

J Oral Maxillofac Surg 2021 Feb 24. Epub 2021 Feb 24.

Professor, Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, Shandong, China. Electronic address:

Purpose: The lingual-based mucoperiosteal flap, a novel flap, was unclear about the effects on the prognosis of surgery for impacted mandibular third molars. This study aimed to compare the lingual- and buccal-based mucoperiosteal flaps with respect to postoperative responses and complications.

Materials And Methods: A systematic review with a meta-analysis was designed and the PubMed, Cochrane Library, EMBASE, and Web of Science databases and Google Scholar from January 1, 2000 to April 30, 2020 were searched for randomized clinical trials. The predictor variable was buccal- or lingual-based flap in the surgery, and the outcome variables were pain, swelling, trismus, operative time, and wound dehiscence. Other study variables were sex and retention depth of impacted teeth. RevMan 5.3 software was used for data analysis. Mean differences or standardized mean differences and risk ratios were computed to assess associations between 2 variables, where statistical significance was set at P < .05.

Results: Seven publications met the inclusion criteria, contributing 370 subjects who had 590 teeth removed to sample. The lingual-based flap failed to significantly reduce postoperative discomfort. However, subgroup analysis revealed that subjects who underwent comma flap (a type of lingual-based flap) surgeries complained of milder pain than those who underwent buccal-based flap surgeries on day 1 (mean difference = -1.18, 95% confidence interval [CI] [-1.53, -0.83], P < .001) and day 7 (mean difference = -1.80, 95% CI [-2.13, -1.48], P < .001) after surgery. Significant differences were also observed on days 1, 3, and 7 regarding postoperative swelling and trismus (P < .01). In addition, the lingual-based flap was reported to cause a significantly lower rate of wound dehiscence (relative risk = 0.46, 95% CI [0.30, 0.69], P = .0002).

Conclusions: The lingual-based flap was associated with better primary wound closure in third molar removal. The comma flap, as a subtype, was preferable for relieving postoperative pain, swelling, and trismus over the buccal-based flap.
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http://dx.doi.org/10.1016/j.joms.2021.02.023DOI Listing
February 2021

Comparison between the effects of feeding copper sulphate-treated and untreated rapeseed cake containing high glucosinolates on rumen fermentation, nutrient digestion and nitrogen metabolism in steers.

J Anim Physiol Anim Nutr (Berl) 2021 Mar 25. Epub 2021 Mar 25.

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.

Two trials were carried out to study the effects of copper sulphate (CuSO ) on detoxifying glucosinolates (GLS) in rapeseed cake (RSC) and compare the effects of feeding CuSO -treated and untreated RSC on nutrient digestion and nitrogen (N) metabolism in steers. In Trial 1, different concentrations of CuSO solution (1.6 vs. 3.2 g CuSO ·5H O L ), soaking temperatures (25 vs. 60°C) and drying methods (air drying at 60°C vs. freeze drying) were allocated in a 2 × 2 × 2 factorial arrangement in vitro. In Trial 2, six steers and dietary inclusions of untreated RSC (control), CuSO -treated RSC and CuSO -added RSC were assigned in a replicated 3 × 3 Latin square design. CuSO treatment in vitro decreased the contents of GLS and thiocyanate (TC) in RSC (p < 0.001). The total amount of GLS and TC decreased by 62.7-68.5% for all treatments. The animal trial showed that CuSO -treated RSC inclusion decreased ruminal concentration of valerate (p < 0.01), whereas it did not affect ruminal pH, ammonia N and total volatile fatty acids. Compared with the control, feeding CuSO -treated or CuSO -added RSC had no effect on plasma concentrations of triiodothyronine and thyroxine, N excretion and N retention. CuSO -treated RSC tended to increase neutral detergent fibre digestibility (p = 0.072) and urinary excretion of urea (p = 0.056). Urinary excretion of purine derivatives (p = 0.076) and rumen microbial N supply (p = 0.084) tended to decrease when feeding CuSO -treated RSC versus control. TC was found to be the only metabolite of GLS in rumen fluid, plasma and urine. It was feasible to detoxify GLS in RSC using low CuSO at room temperature. However, feeding CuSO -treated or CuSO -added RSC had minor effects on rumen fermentation, nutrient digestion and N metabolism in steers. CuSO treatment on RSC for feeding steers seems to be unnecessary.
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http://dx.doi.org/10.1111/jpn.13519DOI Listing
March 2021

GFAP hyperpalmitoylation exacerbates astrogliosis and neurodegenerative pathology in PPT1-deficient mice.

Proc Natl Acad Sci U S A 2021 Mar;118(13)

Institute of Psychiatry and Neuroscience, Xinxiang Medical University, 453000 Xinxiang, China;

The homeostasis of protein palmitoylation and depalmitoylation is essential for proper physiological functions in various tissues, in particular the central nervous system (CNS). The dysfunction of PPT1 (PPT1-KI, infantile neuronal ceroid lipofuscinosis [INCL] mouse model), which catalyze the depalmitoylation process, results in serious neurodegeneration accompanied by severe astrogliosis in the brain. Endeavoring to determine critical factors that might account for the pathogenesis in CNS by palm-proteomics, glial fibrillary acidic protein (GFAP) was spotted, indicating that GFAP is probably palmitoylated. Questions concerning if GFAP is indeed palmitoylated in vivo and how palmitoylation of GFAP might participate in neural pathology remain unexplored and are waiting to be investigated. Here we show that GFAP is readily palmitoylated in vitro and in vivo; specifically, cysteine-291 is the unique palmitoylated residue in GFAP. Interestingly, it was found that palmitoylated GFAP promotes astrocyte proliferation in vitro. Furthermore, we showed that PPT1 depalmitoylates GFAP, and the level of palmitoylated GFAP is overwhelmingly up-regulated in PPT1-knockin mice, which lead us to speculate that the elevated level of palmitoylated GFAP might accelerate astrocyte proliferation in vivo and ultimately led to astrogliosis in INCL. Indeed, blocking palmitoylation by mutating cysteine-291 into alanine in GFAP attenuate astrogliosis, and remarkably, the concurrent neurodegenerative pathology in PPT1-knockin mice. Together, these findings demonstrate that hyperpalmitoylated GFAP plays critical roles in regulating the pathogenesis of astrogliosis and neurodegeneration in the CNS, and most importantly, pinpointing that cysteine-291 in GFAP might be a valuable pharmaceutical target for treating INCL and other potential neurodegenerative diseases.
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http://dx.doi.org/10.1073/pnas.2022261118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020761PMC
March 2021

Association of dietary calcium, magnesium, and vitamin D with type 2 diabetes among US adults: National health and nutrition examination survey 2007-2014-A cross-sectional study.

Food Sci Nutr 2021 Mar 27;9(3):1480-1490. Epub 2021 Jan 27.

Department of Food Engineering Faculty of Engineering University of Gezira Wad Medani Sudan.

Higher dietary intake of calcium (Ca), magnesium (Mg), and vitamin D has been associated with reduced risk of type 2 diabetes (T2DM), and a higher intracellular ratio of Ca to Mg leads to insulin resistance. Previous epidemiological studies did not examine the combined effects of dietary Ca, Mg, and vitamin D as well as ratio of Ca to Mg with T2DM. Therefore, we assessed the relationship between dietary intakes of Mg, Ca, and vitamin D (using 24-hr recalls) individually and in composite and T2DM in the National Health and Nutrition Examination Survey 2007-2014, which involved 20,480 adults (9,977 men and 10,503 women) with comprehensive information on related nutrients, and anthropometric, demographic, and biomarker variables using multivariable logistic regression. The results indicated that dietary calcium at Q3 (812 mg/day) was significantly linked with T2DM in women (OR: 1.30; 95% CI: 1.02, 1.65). Dietary vitamin D at Q3 (5.25 μg/day) significantly reduced the odds of T2DM by 21% in men (OR: 0.79; 95% CI: 0.64, 0.98). This is an interesting study that has important implications for dietary recommendations. It is concluded that US adults having dietary Ca below the RDA were associated with increased risk of T2DM in all population and women, while higher ratio of Ca to Mg was associated with increased risk of T2DM in all population and increased vitamin D intake is related to decreased risk of T2DM in men. Moreover, further research is needed to make more definitive nutritional recommendations.
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http://dx.doi.org/10.1002/fsn3.2118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958525PMC
March 2021

Complexation of β-lactoglobulin with gum arabic: Effect of heat treatment and enhanced encapsulation efficiency.

Food Sci Nutr 2021 Mar 18;9(3):1399-1409. Epub 2021 Jan 18.

School of Food Science and Biotechnology Zhejiang Gongshang University Hangzhou China.

Heat treatment is widely used in food industry. Proteins and polysaccharides as important natural polymers in food, under heat treatment, the interactions between them could mediate the conformation and functional properties of proteins. Thermally induced β-lactoglobulin-gum arabic complexes (β-Lg-GA) were fabricated, and the effect of heat treatment on physicochemical properties of the complexes was systematically investigated. The average particle size of β-Lg-GA complexes decreased with temperature increased, at 85°C, a smaller size of 273 nm was obtained. A saturated adsorption of GA was found when mass ratio of β-Lg/GA was <1:2. At pH = 4.2-7.0, electrostatic attraction between β-Lg and GA was low and a fairly constant turbidity was observed, the formed composite particles had good stability to the pH value. Through UV, fluorescence, and FTIR spectroscopy, it was found that formation of the nanoparticles relied on thermal denaturation and aggregation of protein, the electrostatic, hydrophobic, and hydrogen bonding interactions between β-Lg and GA were also important. Scanning electron microscope further indicated β-Lg and GA had good compatibility, and the complexes had a spherical core-shell structure at molecular level. In addition, these prepared natural nanoparticles by heat treatment show significantly higher encapsulation efficiency for (-)-epigallocatechin-3-gallate (EGCG) than that of unheated, thus could be used as a promising carrier for biologically active substances.
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http://dx.doi.org/10.1002/fsn3.2103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958567PMC
March 2021

Preparation of β-lactoglobulin/gum arabic complex nanoparticles for encapsulation and controlled release of EGCG in simulated gastrointestinal digestion model.

Food Chem 2021 Aug 8;354:129516. Epub 2021 Mar 8.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, People's Republic of China. Electronic address:

In this work, the β-lactoglobulin/gum arabic (β-Lg-GA) complexes were prepared to encapsulate epigallocatechin gallate (EGCG), forming β-Lg-GA-EGCG complex nanoparticles with an average particle size of 133 nm. The β-Lg-GA complexes exhibited excellent encapsulation efficiency (84.5%), and the antioxidant performance of EGCG in vitro was improved after encapsulation. It was recorded that 86% of EGCG could be released in simulated intestinal fluid after 3 h of digestion, much faster than that in simulated gastric fluid, indicating that the β-Lg-GA complexes were effective in enhancing EGCG stability, which was confirmed using SDS-PAGE and SEM. Further spectrum results demonstrated that various intramolecular interactions including electrostatic, hydrophobic and hydrogen bonding interactions contribute to the formation of β-Lg-GA-EGCG complex nanoparticles. Also, XRDexperiments indicated that EGCG was successfully encapsulated by β-Lg-GA complexes. Therefore, the β-Lg-GA complexes hold great potentials in the protective delivery of sensitive bioactives.
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http://dx.doi.org/10.1016/j.foodchem.2021.129516DOI Listing
August 2021

Insights on the mechanism of Fe doped ZnO for tightly-bound extracellular polymeric substances tribo-catalytic degradation: The role of hydration layers at the interface.

Chemosphere 2021 Mar 3;276:130170. Epub 2021 Mar 3.

Department of Chemistry, Dalian University of Technology, Dalian, 116024, PR China.

The control of interfacial microbial pollution is of great significance for water safety. Herein, the tribo-catalysis ability of zinc oxide (ZnO) has been investigated, which can realize the control of tightly-bound extracellular polymeric substances (T-EPS) in water under dark environment. The DFT calculation proves the Fe doping introduces the impurity level and decreases the work function from 5.071 eV to 5.045 eV, improves the charge separation of ZnO, and eventually enhances the catalytic reaction efficiency. Characterizing the catalytic reaction process by three-dimensional fluorescence (3D EEM) and fluorescence regional integration (FRI) method, it is found that the T-EPS solution can be degraded 75.8% by Fe-ZnO in 12 min, while ZnO can only degrade 32.2%. Combining with high-resolution scanning probe microscope (HR-SPM) and attenuated total reflection method (ATR-FTIR), hydration layers consist with hydroxyl layer (∼0.23 nm) and water molecular layer (∼0.27 nm) are observed at the interface between Fe-ZnO and T-EPS solution, and terminal hydroxyl group (OH) is considered to be the active site for the generation of radicals. This study provides an idea for exploring the mechanism of tribo-catalytic reaction and shows its application prospect in the field of microbial inhibition in water.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130170DOI Listing
March 2021

[Determination Method and Characteristics of Particle Size Multiplier of PM in Road Dust].

Huan Jing Ke Xue 2021 Apr;42(4):1642-1648

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.

The particle size multiplier is a valuable parameter for depicting the particle size distribution characteristics of road dust and calculating road dust emissions. In order to realize the localization of the particle size multiplier, the AP-42 and TRAKER methods were used for sampling on typical and different types of roads in Baoding in March 2019. Then, the particle size multiplier of road dust PM () was calculated using the correction formula, and the characteristics were analyzed. The results indicated:① The obtained separately by AP-42 and TRAKER were 0.21 g·VKT and 0.23 g·VKT on average, which correlated well, with a high correlation coefficient of 0.6. The PM emission factors calculated using the of the different methods were almost at the same value, indicating that TRAKER method based on a laser sensor could measure and calculate the and could be directly used to obtain the particle size multiplier or be converted using the fitting equation. ② The characteristics of the in Baoding were ranked as:Expressway
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http://dx.doi.org/10.13227/j.hjkx.202009051DOI Listing
April 2021

Subarachnoid hemorrhage after full endoscopic transforaminal lumbar interbody fusion: a case report.

Br J Neurosurg 2021 Mar 19:1-6. Epub 2021 Mar 19.

Department of Orthopaedics, Xuzhou Central Hospital, Xuzhou Clinical School of Xuzhou Medical University, Xuzhou Clinical College of Nanjing Medical University, Xuzhou, China.

Purpose: Intracranial hemorrhage such as subarachnoid hemorrhage (SAH) is a rare but severe complication of spinal surgery. Current case reports of open lumbar surgery are typically accompanied by intraoperative dural tears and cerebrospinal fluid (CSF) leakage. We report a case of non-aneurysmal SAH without CSF leakage after full endoscopic transforaminal lumbar interbody fusion (FE-TLIF).

Design: Case report and literature review.

Results: A 62-year-old male patient underwent FE-TLIF for L4/5 lumbar spinal stenosis. There was no intraoperative dural tear or postoperative CSF leakage. The patient reported neck pain immediately after the surgery. Around 12 h after the surgery, the patient complained of mild headaches. One day after the surgery, the patient reported severe headaches, accompanied by nausea and vomiting. CT showed a high-density shadow in part of the sulcus and cistern, suggesting SAH. No apparent neurological symptoms were present. The patient's condition improved after conservative treatment including bed rest, fluid infusions, and blood pressure control. Twelve days after the surgery, CT and MRA of the brain showed no hemorrhage and the patient was discharged.

Conclusion: This case was among the first that developed SAH without CSF leakage after FE-TLIF. Although the underlying pathologic mechanism is unknown, epidural hypertension may be a possible cause of the hemorrhage. Timely CT or magnetic resonance imaging (MRI) examinations may help to detect this complication and initiate early treatment.
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http://dx.doi.org/10.1080/02688697.2021.1902473DOI Listing
March 2021

The Electrolysis of Anti-Perovskite Li OHCl for Prelithiation of High-Energy-Density Batteries.

Angew Chem Int Ed Engl 2021 Mar 15. Epub 2021 Mar 15.

Beijing Advanced Innovation Center for Soft Matter Science and Engineering, State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing, 100029, China.

Anti-perovskite type Li OHCl was previously studied as a solid-state Li conductor. Here, we report that the Li OHCl can be electrolyzed at 3.3 V or 4.0 V, with the creation of O /HCl gases and the release of 2 equiv. Li via two different decomposition routes, depending on the acidity of electrolyte. In the electrolyte with trace acid, the Li OHCl is oxidized at a constant voltage of 3.3 V. In neutral electrolyte, the oxidization of Li OHCl starts at 4.0 V, but the produced HCl will increase the acidity of electrolyte and lead to a voltage drop to 3.3 V for the electrolysis of Li OHCl. The electrolysis of Li OHCl delivers a lithium releasing capacity as high as 810 mAh g , with an equivalent Li-deposition or Li-intercalation on anode, making it a promising candidate as a Li reservoir for prelithiation of anode. Using Li OHCl as the lithium source, silicon-carbon (Si@C) composite anode can be effectively prelithiated. The full cells composed of LiNi Mn Co O (NMC811) cathode and prelithiated Si@C anode exhibited increased capacities with the increment of prelithiation dosages.
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http://dx.doi.org/10.1002/anie.202102605DOI Listing
March 2021

Sodium Houttuyfonate Inhibits Bleomycin Induced Pulmonary Fibrosis in Mice.

Front Pharmacol 2021 25;12:596492. Epub 2021 Feb 25.

The Second Hospital of Dalian Medical University, Dalian, China.

Pulmonary fibrosis (PF) could severely disrupt the normal lung architecture and function with fatal consequences. Currently, there is no effective treatment for PF or idiopathic pulmonary fibrosis (IPF). The aim of this study was to investigate the effects of Sodium Houttuyfonate (SH) on bleomycin (BLM) induced PF mice model. Our results indicated that SH could attenuate BLM induced lung injury by reducing the inflammation, fibrogenesis and lung/body weight ratio. The proposed mechanisms for the protective effects of SH include: 1) improvement of pulmonary function in BLM mice, for instance, it can elevate the vital capacity (VC), increase the forced expiratory flow at 50% of forced vital capacity (FEF50) and improve other pulmonary function indices; 2) inhibition of collagen formation in BLM mice; 3) attenuation of the elevation of inflammatory cytokines, such as interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α), which are triggered by BLM administration; 4) reduction of the mRNA level and protein production of transforming growth factor-β1 (TGF-β1) in BLM mice. Furthermore, it was found that the protective effects of SH against BLM induced PF in mice was comparable to that of prednisone acetate (PA) tablets, a widely used drug for immunological diseases. Although Thunb has been widely used in China for lung infection and inflammation, the mechanism has not yet been fully elucidated. Our study provides the evidence that SH is an effective compound against pulmonary injury, irritation and fibrogenesis.
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http://dx.doi.org/10.3389/fphar.2021.596492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947865PMC
February 2021

Epirubicin-induced Kounis syndrome.

BMC Cardiovasc Disord 2021 Mar 12;21(1):133. Epub 2021 Mar 12.

Department of Cardiology, Peking University People's Hospital, Beijing, China.

Background: Kounis syndrome is an acute coronary syndrome that appears in the setting of anaphylactic reaction or hypersensitivity. Many drugs and environmental exposures have been identified as potential offenders, and diagnosis and treatment can be challenging.

Case Presentation: A 62-year-old man with recurrent bladder cancer underwent an intra-iliac artery epirubicin injection. After the injection, he developed chest pain and a systemic allergic reaction, with electrocardiographic alterations and elevated troponin-I levels. Emergent coronary angiography showed right coronary artery spasm and no stenosis of the other coronary arteries. This reaction was considered compatible with an allergic coronary vasospasm. A diagnosis of Kounis syndrome was made.

Conclusions: Kounis syndrome is common, but a prompt diagnosis is often not possible. This case is the first to suggest that an intraarterial epirubicin injection could potentially be one of its triggers. All physicians should be aware of the pathophysiology of this condition to better recognize it and start appropriate treatment; this will prevent aggravation of the vasospastic cardiac attacks and yield a better outcome.
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http://dx.doi.org/10.1186/s12872-021-01936-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953621PMC
March 2021

Gender Differences in the Association Between Serum Uric Acid and Arteriosclerotic Cardiovascular Risk Among Chinese Type 2 Diabetes Mellitus Patients.

Int J Gen Med 2021 1;14:687-695. Epub 2021 Mar 1.

Department of General Practice, Zhongshan Hospital of Fudan University, Shanghai, 200030, People's Republic of China.

Background And Objectives: Serum uric acid (sUA) level has been reported to be associated with arteriosclerotic cardiovascular risk, yet remains poorly defined in Chinese type 2 diabetes patients. The purpose of the current study was to evaluate gender differences in the association between sUA level and arteriosclerotic cardiovascular risk in Chinese type 2 diabetes patients.

Methods: The cross-sectional study was conducted in six community health service centers in Shanghai, China from December 2014 to December 2016. A stratified random sampling method was used to recruit participants. From a total of 3977 type 2 diabetic patients, 2537 were included for the analysis of the association between sUA level and arteriosclerotic cardiovascular risk. Clinical and biochemical data were obtained from participants. Arteriosclerotic cardiovascular risk was evaluated by the ten-year risk profile for arteriosclerotic cardiovascular disease. The associations between sUA level and arteriosclerotic cardiovascular risk were assessed via multiple logistic regression.

Results: Of the 2537 participants, the average sUA level was 317±77umol/L in men and 294±73 umol/L in women, and 54.8% (1391/2537) of participants had high ten-year risk of arteriosclerotic cardiovascular disease (ASCVD), which was ≥20%. High ten-year risk of ASCVD odds ratio was increased by 1.596 (95% CI, 1.113-2.289, for trend 0.004) comparing fourth to first quartiles of sUA in women. However, no significant association was found between sUA and high ten-year risk of ASCVD in men.

Conclusion: This community-based study indicated that sUA levels were independently associated with high ten-year risk of ASCVD in women with type 2 diabetes mellitus, but not in men.
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http://dx.doi.org/10.2147/IJGM.S300196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935449PMC
March 2021