Publications by authors named "Jian Deng"

171 Publications

Morphological and molecular identification of two new species (Ascomycota, Pleosporaceae) in section Radicina from China.

MycoKeys 2021 9;78:187-198. Epub 2021 Apr 9.

Institute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi, 530007, China Institute of Plant Protection, Guangxi Academy of Agricultural Sciences and Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests Nanning China.

The fungal genus was distributed widely and found in different habitats such as plant or indoor environment. During an investigation into this genus in China, two new species, and were respectively isolated from diseased leaves of and , which both belonged to Umbelliferae. Phylogenetically, they were determined as new species belonging in the section Radicina of based on the combined four gene fragments of ITS, , and . Morphologically, the two species were illustrated and compared with other relevant Alternaria species in section Radicina.
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http://dx.doi.org/10.3897/mycokeys.78.64853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052298PMC
April 2021

Spontaneous response of a self-organized fish school to a predator.

Bioinspir Biomim 2021 Apr 30. Epub 2021 Apr 30.

Department of Mechanics, Zhejiang University, 38 Zheda Road, Hangzhou, Hangzhou, 310027, CHINA.

While the collective movements of fish schools evading predators in nature are complex, they can be fundamentally represented by simplified mathematical models. Here we develop a numerical model, which considers self-propelled particles (SPP) subject to phenomenological behavioural rules and the hydrodynamic interactions between individuals. We introduce a predator in this model, to study the spontaneous response of a group of simulated fish to the threat. A self-organized fish school with a milling pattern is considered, which was expected to be efficient to evade the threat of predators. Four different attack tactics are adopted by the predator. We find that the simulated fish form transiently smaller structures as some prey individuals split from the main group, but eventually they will re-organize, sometimes into sub groups when the simulated predator approaches the fish school unidirectionally or take a reciprocating action. As the predator is programmed to target the centroid, the school ends in a gradually enlarging circle. For the fourth tactic, as the predator chases its nearest prey, the fish school's response varies with the predator's delay factor. Moreover, the average speed of the group and the distance between individuals have also been studied, both demonstrating that the fish school is able to respond spontaneously to the predator's invasion. We demonstrate that the currently adopted model can predict prey-predator interactions.
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http://dx.doi.org/10.1088/1748-3190/abfd7fDOI Listing
April 2021

Socio-economic status, resilience, and vulnerability of households under COVID-19: Case of village-level data in Sichuan province.

PLoS One 2021 29;16(4):e0249270. Epub 2021 Apr 29.

Center for Trans-Himalaya Studies, Leshan Normal University, Leshan, Sichuan, China.

This paper investigates economic impacts of COVID-19 on households based on differences in the socio-economic status (SES). We determine the household-level effects of the COVID-19 shock using income sources, types of industries, communities' resilience, household susceptibility, and relevant policy measures. For this purpose, we used primary data of 555 households collected through snowball sampling technique using an online survey questionnaire from different villages mostly located in Sichuan Province, China. Using step-wise binary logistic regression analysis, we estimated and validated the model. Results suggest the use of SES as a better measure for understanding the impacts of COVID-19 on different households. We find that households with low SES tend to depend more on farmland income and transfer payments from the government. Contrarily, high SES households focus more on business and local employment as sources of income generation. Poor households were less resilient and more likely to fall back into poverty due to COVID-19, while the opposite stands true for non-poor households with high SES. Based on the estimations, policies encouraging employment and businesses complemented with loans on lower interest rates are recommended, which may increase the SES, thus minimizing vulnerability and enhancing the households' resilience towards poverty alleviation and economic shocks.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0249270PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084142PMC
May 2021

SARS-CoV-2 ORF9b antagonizes type I and III interferons by targeting multiple components of the RIG-I/MDA-5-MAVS, TLR3-TRIF, and cGAS-STING signaling pathways.

J Med Virol 2021 Apr 29. Epub 2021 Apr 29.

Key Laboratory for Experimental Teratology of Ministry of Education and Advanced Medical Research Institute, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

The suppression of types I and III interferon (IFN) responses by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contributes to the pathogenesis of coronavirus disease 2019 (COVID-19). The strategy used by SARS-CoV-2 to evade antiviral immunity needs further investigation. Here, we reported that SARS-CoV-2 ORF9b inhibited types I and III IFN production by targeting multiple molecules of innate antiviral signaling pathways. SARS-CoV-2 ORF9b impaired the induction of types I and III IFNs by Sendai virus and poly (I:C). SARS-CoV-2 ORF9b inhibited the activation of types I and III IFNs induced by the components of cytosolic dsRNA-sensing pathways of RIG-I/MDA5-MAVS signaling, including RIG-I, MDA-5, MAVS, TBK1, and IKKε, rather than IRF3-5D, which is the active form of IRF3. SARS-CoV-2 ORF9b also suppressed the induction of types I and III IFNs by TRIF and STING, which are the adaptor protein of the endosome RNA-sensing pathway of TLR3-TRIF signaling and the adaptor protein of the cytosolic DNA-sensing pathway of cGAS-STING signaling, respectively. A mechanistic analysis revealed that the SARS-CoV-2 ORF9b protein interacted with RIG-I, MDA-5, MAVS, TRIF, STING, and TBK1 and impeded the phosphorylation and nuclear translocation of IRF3. In addition, SARS-CoV-2 ORF9b facilitated the replication of the vesicular stomatitis virus. Therefore, the results showed that SARS-CoV-2 ORF9b negatively regulates antiviral immunity and thus facilitates viral replication. This study contributes to our understanding of the molecular mechanism through which SARS-CoV-2 impairs antiviral immunity and provides an essential clue to the pathogenesis of COVID-19.
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http://dx.doi.org/10.1002/jmv.27050DOI Listing
April 2021

Effect of right-to-left shunt on youth benign paroxysmal positional vertigo: A prospective clinical study.

Clin Neurol Neurosurg 2021 Apr 20;205:106646. Epub 2021 Apr 20.

Department of Neurology, The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong 518020, China. Electronic address:

Objective: To evaluate the correlation between right to left shunt (RLS) and youth benign paroxysmal positional vertigo (YBPPV).

Patients And Methods: Patients with benign paroxysmal positional vertigo (BPPV) and normal control cases younger than 45 years were enrolled at 1:1 ratio. Contrast-enhanced transcranial doppler ultrasound was performed to determine the existence and shunt grading of RLS. Regression analysis was conducted by including other possible risk factors for YBPPV to evaluate whether RLS acts as an independent risk factor.

Results: A total of 174 cases were enrolled, with 87 cases in the YBPPV group and 87 cases in the normal control group. The proportion of existing RLS in the YBPPV group was significantly higher than in the normal control group (65.52% vs. 28.74%, P < 0.001). Regression analysis showed that RLS was an independent risk factor for YBPPV (odds ratio = 2.157, 95% credibility interval: 1.504~3.093, P<0.001). In the YBPPV group, the recurrence of BPPV within 180 days showed no statistical difference between cases with RLS and receiving anti-platelet treatment and cases with RLS and without anti-platelet treatment. In the YBPPV group, the recurrence of BPPV within 180 days in cases without RLS and anti-platelet treatment did not significantly differ from cases with RLS and without anti-platelet treatment and cases with RLS and receiving anti-platelet treatment.

Conclusion: In the present study, RLS resulted as an independent risk factor for YBPPV. This observation could generate hypotheses of the mechanism by which a RLS could induce YBPPV.
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http://dx.doi.org/10.1016/j.clineuro.2021.106646DOI Listing
April 2021

Formation Mechanism of Monodispersed Polysilsesquioxane Spheres in One-Step Sol-Gel Method.

Langmuir 2021 Apr 27. Epub 2021 Apr 27.

The State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.

Monodispersed polysilsesquioxane (PSQ) spheres with diameters from hundreds of nanometers to several microns have been successfully synthesized; however, the knowledge of their formation mechanism still lags behind. Herein, with methyltrimethoxysilane and 3-mercaptopropyl trimethoxysilane as model silicon sources, the formation process of PSQ spheres in the one-step sol-gel method was revealed for the first time by monitoring the time evolution of particle morphology, size, and size distribution via transmission electron microscopy and dynamic light scattering. A four-stage formation mechanism was proposed: rapid hydrolysis of organic silicon source and subsequent oligomer micelle nucleation, fast growing of nuclei particles and formation of their aggregates, followed by a further relatively fast growth of dispersed particles, and finally a slow growth to form monodispersed PSQ spheres. Due to the reversibility of hydrolysis and condensation reactions, thermodynamically unstable particles gradually transformed to hydrolytic monomers/oligomers and then regrew on the thermodynamically stable particles until the concentration of hydrolytic oligomers reached the dissolution equilibrium in the alkaline reaction solution. The variation of growth rate during the formation process and the effects of NHOH concentration on the yield and particle size were investigated to facilitate analyses and understanding of the formation mechanism.
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http://dx.doi.org/10.1021/acs.langmuir.1c00350DOI Listing
April 2021

Symmetry Breaking of Tail-Clamped Filaments in Stokes Flow.

Phys Rev Lett 2021 Mar;126(12):124501

Swedish e-Science Research Centre and Linné FLOW Centre, Department of Engineering Mechanics, KTH, SE-100 44 Stockholm, Sweden.

Symmetry breaking (SB) of fluid-structure interaction problems plays an important role in our understanding of animals' locomotive and sensing behaviors. In this Letter, we study the SB of flexible filaments clamped at one end and placed in a spanwise periodic array in Stokes flow. The equilibrium state of the filament along the streamwise direction loses stability and experiences two-dimensional and then three-dimensional SBs as the spanwise distance increases, or as the filament rigidity reduces. For slightly deformed filaments, the viscous and pressure forces are commensurate, while for extremely deformed filaments the viscous force becomes dominant.
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http://dx.doi.org/10.1103/PhysRevLett.126.124501DOI Listing
March 2021

The effect of fluoxetine on morning blood pressure surge in patients with ischemic stroke: a prospective preliminary clinical study.

Blood Press Monit 2021 Apr 7. Epub 2021 Apr 7.

Department of Neurology, The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, China.

Objective: Our study aimed to evaluate the effect of fluoxetine on morning blood pressure surge (MBPS) in patients with ischemic stroke.

Methods: A total of 30 patients with acute ischemic stroke, 15 in the control group and 15 in the treatment group, were enrolled. Two patients in the control group and one in the treatment group were lost during follow-up. The patients in the treatment group were treated with fluoxetine (20 mg per day for 3 months) based on the treatments in the control group. Baseline characteristics, Patient Health Questionnaire 9 (PHQ-9) items scale score, mean blood pressure and MBPS were evaluated before and after treatment, and the correlation between the improvements of PHQ-9 scale score and MBPS was also analyzed.

Results: The mean SBP and the systolic and diastolic MBPS in the treatment group were significantly lower than that in the control group after 3 months of treatment (P = 0.024, P = 0.022, P = 0.001, respectively). Besides, the improvement of PHQ-9 scale score was significantly higher in the treatment group than in the control group (P = 0.049). There was no significant correlation between the improvement of systolic or diastolic MBPS and the improvement of PHQ-9 scale score in the treatment group (P = 0.289 and P = 0.206, respectively).

Conclusion: Fluoxetine might improve MBPS in patients with ischemic stroke.
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http://dx.doi.org/10.1097/MBP.0000000000000538DOI Listing
April 2021

An evaluation of machine learning classifiers for next-generation, continuous-ethogram smart trackers.

Mov Ecol 2021 Mar 30;9(1):15. Epub 2021 Mar 30.

Centre for Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, Australia.

Background: Our understanding of movement patterns and behaviours of wildlife has advanced greatly through the use of improved tracking technologies, including application of accelerometry (ACC) across a wide range of taxa. However, most ACC studies either use intermittent sampling that hinders continuity or continuous data logging relying on tracker retrieval for data downloading which is not applicable for long term study. To allow long-term, fine-scale behavioural research, we evaluated a range of machine learning methods for their suitability for continuous on-board classification of ACC data into behaviour categories prior to data transmission.

Methods: We tested six supervised machine learning methods, including linear discriminant analysis (LDA), decision tree (DT), support vector machine (SVM), artificial neural network (ANN), random forest (RF) and extreme gradient boosting (XGBoost) to classify behaviour using ACC data from three bird species (white stork Ciconia ciconia, griffon vulture Gyps fulvus and common crane Grus grus) and two mammals (dairy cow Bos taurus and roe deer Capreolus capreolus).

Results: Using a range of quality criteria, SVM, ANN, RF and XGBoost performed well in determining behaviour from ACC data and their good performance appeared little affected when greatly reducing the number of input features for model training. On-board runtime and storage-requirement tests showed that notably ANN, RF and XGBoost would make suitable on-board classifiers.

Conclusions: Our identification of using feature reduction in combination with ANN, RF and XGBoost as suitable methods for on-board behavioural classification of continuous ACC data has considerable potential to benefit movement ecology and behavioural research, wildlife conservation and livestock husbandry.
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http://dx.doi.org/10.1186/s40462-021-00245-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011142PMC
March 2021

Downregulation of lncRNA TSLNC8 promotes melanoma resistance to BRAF inhibitor PLX4720 through binding with PP1α to re-activate MAPK signaling.

J Cancer Res Clin Oncol 2021 Mar 3;147(3):767-777. Epub 2021 Jan 3.

Dermatology and STD Department, Affiliated Hospital of Nantong University, Jiangsu, China.

Purpose: Approximately 60% of patients with melanoma harbor BRAF mutation and targeting BRAF offers enormous advance in the treatment of those patients. Unfortunately, the efficacy of the BRAF inhibitors is usually restricted by the onset of drug resistance. Therefore, better understanding of the adaptive drug resistance mechanisms is essential for the development of alternative therapeutic strategies, and offers more promising measures to promote the short duration of response to BRAF inhibitors.

Methods: The levels of tumor suppressive long noncoding RNA on chromosome 8p12 (TSLNC8) were evaluated by qPCR. The MTT assay, colony formation assay, apoptosis assay, and in vivo xenograft tumor model were performed to assess the functions of TSLNC8 on drug resistance. Western blotting, RNA pull-down, and RNA immunoprecipitation (RIP) assays were applied to investigate the mechanisms of TSLNC8 in melanoma.

Results: Herein, our findings demonstrate that TSLNC8 is significantly downregulated in BRAF inhibitor-resistant melanoma tissues and cells. Moreover, downregulation of TSLNC8 in BRAF inhibitor sensitive cells reduces the toxicity response to BRAF inhibitor PLX4720, and inhibits apoptosis of melanoma cells-treated with PLX4720. Further assay elucidates that TSLNC8 can bind with the catalytic subunit of protein phosphatase 1α (PP1α) to regulate its distribution, and Downregulation of TSLNC8 results in PP1α cytoplasmic accumulation, thus re-activating the MAPK signaling. Eventually, the overexpression of TSLNC8 in BRAF inhibitor PLX4720-resistant melanoma cells restores the sensitive to BRAF inhibitor.

Conclusion: Collectively, our research provides a compelling rationale for resistance to BRAF inhibitor in melanoma, and the patient might benefit from the combinatorial therapy of BRAF inhibitors and lncRNA TSLNC8.
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http://dx.doi.org/10.1007/s00432-020-03484-4DOI Listing
March 2021

Intradermal delivery of receptor-binding domain of SARS-CoV-2 spike protein with dissolvable microneedles to induce humoral and cellular responses in mice.

Bioeng Transl Med 2020 Dec 12:e10202. Epub 2020 Dec 12.

Department of Biomedical Engineering City University of Hong Kong Hong Kong SAR China.

The S1 subunit of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein contains an immunogenic receptor-binding domain (RBD), which is a promising candidate for the development of a potential vaccine. This study demonstrated that intradermal delivery of an S-RBD vaccine using a dissolvable microneedle skin patch can induce both significant B-cell and significant T-cell responses against S-RBD. Importantly, the outcomes were comparable to that of conventional bolus injection.
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http://dx.doi.org/10.1002/btm2.10202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744900PMC
December 2020

Electron-Accepting Micelles Deplete Reduced Nicotinamide Adenine Dinucleotide Phosphate and Impair Two Antioxidant Cascades for Ferroptosis-Induced Tumor Eradication.

ACS Nano 2020 11 6;14(11):14715-14730. Epub 2020 Nov 6.

School of Pharmaceutical Science & Technology, Tianjin Key Laboratory for Modern Drug Delivery & High Efficiency, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, China.

Ferroptotic antitumor therapy has been compromised by various intracellular antioxidants, particularly glutathione and thioredoxin. Both are cofactors of glutathione peroxide 4 (GPX4) that act against oxidative stress catalyzing the reduction of lipid peroxides. It was postulated that tailored polymer micelles could enhance ferroptotic antitumor efficacy diminishing glutathione and thioredoxin under hypoxia. The aim was to engineer hypoxia-responsive micelles for selective enhancement of ferroptotic cell death in solid tumor. The polymer contains hydrophilic poly(ethylene glycol) (PEG) that is linked by azobenzene linker with nitroimidazole-conjugated polypeptide. The tailored polymer could self-assemble into nanoscale micelles to encapsulate RAS-selective lethal small molecule 3, a covalent GPX4 inhibitor. Under hypoxia, the azobenzene moiety enabled PEG shedding and enhanced micelles uptake in 4T1 cells. Likewise, the nitroimidazole moiety was reduced by the overexpressed nitroreductase with reduced nicotinamide adenine dinucleotide phosphate (NADPH) as the cofactor, resulting in transient depletion of NADPH. This impaired both the glutathione and thioredoxin redox cycle, leading to diminished intracellular glutathione and thioredoxin. The selective potency of ferroptotic micelles in depleting NADPH, glutathione and thioredoxin was further verified in the 4T1 tumor xenograft mice model. This work highlights the role of hypoxia-responsive polymers in enhancing the potency of ferroptotic inducers against solid tumors without additional side effects to healthy organs.
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http://dx.doi.org/10.1021/acsnano.0c00764DOI Listing
November 2020

Determination of Dynamic Interfacial Tension during the Generation of Tiny Droplets in the Liquid-Liquid Jetting Flow Regime.

Langmuir 2020 Nov 4;36(45):13633-13641. Epub 2020 Nov 4.

State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.

Liquid-liquid dispersion coupled with droplet formation and mass transfer of surfactants is one of the most typical phenomena in many chemical processes. As in every aspect of this process, the interfacial tension variation caused by the unsaturated adsorption of surfactants on the droplet surface plays an important role. This article focuses on microdroplet formation and the dynamic interfacial behavior of surfactants in the jetting regime. In a capillary embedded step T-junction device, controllable preparation of monodisperse droplets is achieved, and a correlation for predicting droplet sizes is established. A method for measuring the dynamic interfacial tension is provided. Mass transfer coefficients are then calculated for Tween 20 during the droplet formation process by a semiempirical correlation. The results indicate that dynamic interfacial tensions are lower than those obtained when the surfactant is adsorbed to equilibrium. Based on the tip-streaming phenomenon, mass transfer coefficients for Tween 20 can reach up to ∼10 m/s, higher than those obtained in coaxial microfluidic devices. All the preliminary results shed light on the nature of droplet formation and will be of significance for application in industrial apparatuses.
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http://dx.doi.org/10.1021/acs.langmuir.0c02459DOI Listing
November 2020

Organic single crystals of cyano-substituted -phenylene vinylene derivatives as transistors with low surface trap density.

Chem Commun (Camb) 2020 Nov;56(89):13776-13779

State Key Laboratory of Luminescent Materials and Devices, Institute of Polymer Optoelectronic Materials and Devices, South China University of Technology, No. 381 Wushan Road, Tianhe, Guangzhou 510640, P. R. China.

We obtained two high-quality lamellar crystals of cayno-substituted p-phenylene vinylene derivatives and fabricated their transistors. The transistors demonstrated hysteresis-free, low subthreshold swing values of 0.15 and 0.10 V dec-1, and high mobilities of 0.56 and 2.73 cm2 V-1 s-1, respectively. More importantly, the low defect density of 1 per 9.22 × 103 and 1.82 × 104 molecules, respectively, confirmed their high crystal quality.
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http://dx.doi.org/10.1039/d0cc06552hDOI Listing
November 2020

Reaction Pathway and Selectivity Control of Tetraethyl Thiuram Disulfide Synthesis with NaHCO as a pH Regulator.

ACS Omega 2020 Sep 10;5(37):23736-23742. Epub 2020 Sep 10.

The State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.

The selectivity of a chemical reaction is related to the effective utilization of raw materials as well as the cleanliness and economy of the process. Herein, it has been first proposed to synthesize tetraethyl thiuram disulfide (TETD) with sodium bicarbonate as the pH regulator with a reaction selectivity of ∼100%. The existence of a reaction intermediate, a sodium salt of diethyl dithiocarbamoylsulfenic acid (NaEtDTCS), has been proved by experiments and theoretical calculations. The results indicate that TETD can not only be generated from NaEtDTCS oxidized by HO directly, but also from the conjugation of NaEtDTC and NaEtDTCS generated in the first stage of oxidation meanwhile. Accordingly, an oxidation reaction pathway has been proposed. The reaction selectivity with NaHCO or CO as the pH regulator has been compared, and the selectivity control mechanism is discussed. At relatively higher pH values with NaHCO as the pH regulator, peroxidation could be almost avoided.
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http://dx.doi.org/10.1021/acsomega.0c02707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513355PMC
September 2020

Gastrointestinal involvement of COVID-19 and potential faecal transmission of SARS-CoV-2.

J Zhejiang Univ Sci B 2020 Sept.;21(9):749-751

Department of Laboratory Medicine, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.

Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was found initially in Wuhan, China in early December 2019. The pandemic has spread to 216 countries and regions, infecting more than 23310 000 people and causing over 800 000 deaths globally by Aug. 24, 2020, according to World Health Organization (https://www.who.int/emergencies/diseases/ novel-coronavirus-2019). Fever, cough, and dyspnea are the three common symptoms of the condition, whereas the conventional transmission route for SARS-CoV-2 is through droplets entering the respiratory tract. To date, infection control measures for COVID-19 have been focusing on the involvement of the respiratory system. However, ignoring potential faecal transmission and the gastrointestinal involvement of SARS-CoV-2 may result in mistakes in attempts to control the pandemic.
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http://dx.doi.org/10.1631/jzus.B2000253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7495406PMC
September 2020

Efficient Organic Light-Emitting Transistors Based on High-Quality Ambipolar Single Crystals.

ACS Appl Mater Interfaces 2020 Sep 17;12(39):43976-43983. Epub 2020 Sep 17.

State Key Laboratory of Luminescent Materials and Devices, Institute of Polymer Optoelectronic Materials and Devices, South China University of Technology, No. 381 Wushan Road, Tianhe District, Guangzhou 510640, P. R. China.

A cyano-substituted styrene derivative is synthesized and successfully prepared to lamellate single crystals through precisely controlling the crystal growth conditions. The lamellate single crystals with regular edge and smooth surface display intrinsically ordered stacking and high quality, all of which are of importance for high optoelectronic performance. The single-component light-emitting transistors based on the lamellate crystals offer striking device performance in terms of record external quantum efficiency of 2.02%, exceeding the benchmark value in this field. Such organic light-emitting single crystals provide a versatile platform for designing and engineering their structures and optoelectronic properties toward light-emitting devices.
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http://dx.doi.org/10.1021/acsami.0c12842DOI Listing
September 2020

A seven-year surveillance study of the epidemiology, antifungal susceptibility, risk factors and mortality of candidaemia among paediatric and adult inpatients in a tertiary teaching hospital in China.

Antimicrob Resist Infect Control 2020 08 14;9(1):133. Epub 2020 Aug 14.

Department of Laboratory Medicine, the Affiliated Hospital of Southwest Medical University, 25 Taiping street, Luzhou, 646000, P.R. China.

Background: There are no current national estimates of the candidaemia burden in China, and epidemiological candidaemia data from the underdeveloped region of China are lacking.

Methods: A 7-year retrospective study was carried out to analyse the prevalence, species distribution, antifungal susceptibility, risk factors and inpatient mortality of candidaemia among paediatric and adult patients in a regional tertiary teaching hospital in China.

Results: During the seven-year study period, a total of 201 inpatients with candidaemia were identified. The median age of the patients was 65 years (range, 1 day to 92 years), and 114 of the patients (56.7%) were male. The mean annual incidence of candidaemia was 0.26 cases per 1000 admissions (0.42 cases per 1000 paediatric admissions vs 0.24 cases per 1000 adult admissions, P < 0.05). Candida albicans was the most common fungal species (81/201, 40.3%) in all patients, Candida glabrata was the most common fungal species (18/35, 51.4%) in paediatric patients. Most isolates were susceptible to flucytosine (99.0%) and amphotericin B (99.0%), and the activity of antifungal agents against Candida species was no significant difference in satisfaction between paediatric and adult patients (P > 0.05). The all-cause mortality rate was 20.4% (paediatric patients: 11.4% vs adult patients:22.3%, P > 0.05). Fewer univariate predictors of poor outcomes were identified for paediatric patients than for adult patients (4 vs 11 predictors). Respiratory dysfunction and septic shock were independent predictors of 30-day mortality for all patients.

Conclusions: The epidemiological data of candidaemia in paediatric and adult patients are only different in the distributions of Candida species and the mean annual incidence of candidaemia. Flucytosine and amphotericin B can be used as first-choice agents when no antifungal susceptibility test results are available.
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http://dx.doi.org/10.1186/s13756-020-00798-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7429891PMC
August 2020

SARS-CoV-2 infects human neural progenitor cells and brain organoids.

Cell Res 2020 10 4;30(10):928-931. Epub 2020 Aug 4.

School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 3/F, Laboratory Block, 21 Sassoon Road, Pokfulam, Hong Kong, China.

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http://dx.doi.org/10.1038/s41422-020-0390-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399356PMC
October 2020

Epidemiology, antifungal susceptibility, risk factors and mortality of invasive candidiasis in neonates and children in a tertiary teaching hospital in Southwest China.

Mycoses 2020 Jul 20. Epub 2020 Jul 20.

Department of Laboratory Medicine, Affiliated Hospital of Southwest Medical University, Luzhou, China.

Background: Invasive candidiasis (IC) is a major cause of morbidity and mortality in neonates and children; however, little information on the epidemiology of IC in paediatric patients in China is available.

Methods: A 7-year retrospective study was conducted to analyse the prevalence, species distributions, antifungal susceptibility, risk factors and mortality among paediatric inpatients with IC in a regional tertiary teaching hospital in Southwest China.

Results: A total of 86 paediatric inpatients with IC during the seven-year study period were identified, with a mean annual incidence of 1.04 cases per 1000 admissions and a neonatal incidence of 2.72 cases per 1000 admissions. The species distributions of Candida albicans and non-albicans Candida were 48.8% and 51.2%, respectively. The total resistance rates to fluconazole (FCA), itraconazole (ITR) and voriconazole (VRC) were 8.1%, 26.7% and 14.0%, respectively. Age, length of hospital stay, respiratory dysfunction, hospitalisation duration > 30 days and IC due to C albicans and Candida glabrata were associated with neonatal mortality (P < 0.05) according to univariate analyses. Respiratory dysfunction [odds ratio (OR), 50.03; 95% confidence interval (CI), 3.47-721.44; P = 0.004] was the only independent predictor of neonatal mortality. The overall mortality rate was 8.1%, and only neonatal IC patients died, with a neonatal mortality rate of 13.0%.

Conclusions: This report shows that the incidence and mortality of IC among neonatal patients warrants increased attention in Southwest China. Clinical interventions should be actively applied to reduce the incidence and mortality of neonatal infection. Fluconazole was a reasonable choice for the treatment of IC prior to species identification.
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http://dx.doi.org/10.1111/myc.13146DOI Listing
July 2020

Mining of epitopes on spike protein of SARS-CoV-2 from COVID-19 patients.

Cell Res 2020 08 1;30(8):702-704. Epub 2020 Jul 1.

CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, 518055, China.

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http://dx.doi.org/10.1038/s41422-020-0366-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327194PMC
August 2020

Increasing host cellular receptor-angiotensin-converting enzyme 2 expression by coronavirus may facilitate 2019-nCoV (or SARS-CoV-2) infection.

J Med Virol 2020 11 2;92(11):2693-2701. Epub 2020 Jul 2.

Advanced Medical Research Institute, Cheeloo College of Medicine, Shandong University, Jinan, China.

The ongoing outbreak of a new coronavirus (2019-nCoV, or severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) has caused an epidemic of the acute respiratory syndrome known as coronavirus disease (COVID-19) in humans. SARS-CoV-2 rapidly spread to multiple regions of China and multiple other countries, posing a serious threat to public health. The spike (S) proteins of SARS-CoV-1 and SARS-CoV-2 may use the same host cellular receptor, angiotensin-converting enzyme 2 (ACE2), for entering host cells. The affinity between ACE2 and the SARS-CoV-2 S protein is much higher than that of ACE2 binding to the SARS-CoV S protein, explaining why SARS-CoV-2 seems to be more readily transmitted from human to human. Here, we report that ACE2 can be significantly upregulated after infection of various viruses, including SARS-CoV-1 and SARS-CoV-2, or by the stimulation with inflammatory cytokines such as interferons. We propose that SARS-CoV-2 may positively induce its cellular entry receptor, ACE2, to accelerate its replication and spread; high inflammatory cytokine levels increase ACE2 expression and act as high-risk factors for developing COVID-19, and the infection of other viruses may increase the risk of SARS-CoV-2 infection. Therefore, drugs targeting ACE2 may be developed for the future emerging infectious diseases caused by this cluster of coronaviruses.
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http://dx.doi.org/10.1002/jmv.26139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7300907PMC
November 2020

Classification of breast density categories based on SE-Attention neural networks.

Comput Methods Programs Biomed 2020 Sep 30;193:105489. Epub 2020 Apr 30.

Department of Radiology, First Hospital of Shanxi Medical University, Taiyuan, China.. Electronic address:

Background And Objective: Breast density (BD) is an independent predictor of breast cancer risk factor. The automatic classification of BD has yet to resolve. In this paper, we propose an improved convolutional neural network (CNN) framework that integrates innovative SE-Attention mechanism to learn discriminative features, aiming for automatic BD classification in mammography.

Methods: A new benchmarking dataset was constructed from 18157 BD images, manually segmented into 4 levels based on Breast Imaging and Reporting Data System (BI-RADS): A (fatty), B (fibro-glandular), C (heterogeneously dense) and D (extremely dense). The proposed method consists of three main phases: (i) data enhancement and normalization of breast images (ii) SE-Attention training for feature re-calibration and fusion to better classify density and (iii) designing the auxiliary loss. We adopt an attention approach where SE-Attention mechanism is used to learn the density features, which is different from previous works.

Results: Experimental results demonstrate that the proposed framework obtains higher classification accuracy than the original network, such as Inception-V4, ResNeXt, DenseNet, increasing the performance from 89.97% to 92.17%, 89.64% to 91.57%, 89.20% to 91.79% respectively. Among them, improved Inception-V4 possesses the highest accuracy meanwhile DenseNet improves in the largest extent, both the original and improved methods are more effective than other state-of-the-art image descriptors regarding classification.

Conclusions: We insist that our method will help radiologists provide reliable BD diagnostic services at the expert level, allowing them to focus on patients who are really in need.
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http://dx.doi.org/10.1016/j.cmpb.2020.105489DOI Listing
September 2020

Effect of coagulation function on cerebral microbleeds in intracerebral hemorrhage.

Brain Behav 2020 06 19;10(6):e01634. Epub 2020 Apr 19.

Department of Neurology, Shenzhen People's Hospital, The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen, Guangdong, China.

Objective: Our study aimed to confirm whether coagulation function of patients presenting with intracerebral hemorrhage (ICH) was associated with onset of cerebral microbleeds (CMBs).

Methods: A total of 174 patients with basal ganglia ICH were divided into CMBs and non-CMBs groups. Indicators of coagulation function and other clinical data that included fibrinogen (FBI), prothrombin time (PT), activated partial thromboplastin time (APTT), and the international normalized ratio (INR) were compared by univariate and multivariate analysis between the two groups. A receiver operating characteristic (ROC) curve was plotted to determine the predictive value of coagulation function indicators for CMBs.

Results: Univariate analysis showed that APTT levels was significantly higher in the CMBs group than the non-CMBs group (30.20 ± 5.18 vs. 27.95 ± 4.19; p = .004), while there was no significant difference between PT, INR, and FBI. The proportion of male patients in the CMBs group was significantly higher than the non-CMBs group (76.58% vs. 52.38%, p = .001). Multifactor logistic regression analysis demonstrated that APTT and male gender were independent risk factors for CMBs in patients with ICH (OR 1.100, 95% CI: 1.026-1.180, p = .008; OR 2.957, 95% CI: 1.500-5.826, p = .002; respectively). ROC curve analysis indicated that the area under the curve of APTT and male gender for CMBs in patients with ICH was 0.641 and 0.621, respectively (p = .002 and .008; respectively).

Conclusion: APTT was an independent risk factor for CMBs in patients with ICH.
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http://dx.doi.org/10.1002/brb3.1634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303373PMC
June 2020

Deep cerebral venous blood flow monitoring by transcranial doppler ultrasonography in the treatment of cerebral venous sinus thrombosis: A case report.

J Clin Ultrasound 2021 Feb 11;49(2):154-158. Epub 2020 Apr 11.

Department of Neurology, Shenzhen People's Hospital, The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen, Guangdong, China.

We report the case of a patient in whom we used deep cerebral venous blood flow monitoring by transcranial Doppler ultrasonography to monitor the effect of anticoagulation therapy on cerebral venous sinus thrombosis. The blood flow velocity of deep cerebral veins increased in the early stage of cerebral venous sinus thrombosis, then gradually decreased to the normal level as the disease improved. Moreover, the recovery of the blood flow velocity of deep cerebral veins occurred earlier than the morphological recovery demonstrated by magnetic resonance venography.
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http://dx.doi.org/10.1002/jcu.22840DOI Listing
February 2021

Extra-Wide Lane Ambiguity Resolution and Validation for a Single Epoch Based on the Triple-Frequency BeiDou Navigation Satellite System.

Sensors (Basel) 2020 Mar 10;20(5). Epub 2020 Mar 10.

School of Remote Sensing and Geomatics Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China.

The ambiguity resolution (AR) and validation of the global navigation satellite system (GNSS) have been challenging tasks for some decades. Considering the reliability problem of extra-wide-lane (EWL) ambiguity in the triple-carrier ambiguity resolution (TCAR), a method for validating the reliability of the EWL ambiguity using a single epoch was proposed for the BeiDou Navigation Satellite System (BDS). For the initial EWL ambiguity, obtained using a rounding estimator with a geometry-free (GF) model, the double-difference ionospheric delay was first estimated to construct a relative positioning model with an initial fixed ambiguity. Second, based on the theory of gross error detection and the AR characteristics of EWL, the second-best ambiguity candidate was constructed. Finally, among the two sets of ambiguities, the one with the smaller posterior variance was taken as the reliable ambiguity. The study showed that, for a single epoch, when only one or two satellites had incorrect ambiguities, the AR success rate after ambiguity validation and correction could reach 100% for medium baselines. For long baselines, due to the increase of atmospheric error, the validation was affected to some extent. However, the AR success rates for two long baselines increased from 96.82% and 98.44% to 98.80% and 99.67%, respectively.
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http://dx.doi.org/10.3390/s20051534DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085751PMC
March 2020

Identification and Characterization of Causing Leaf Spot and Blight on .

Mycobiology 2020 29;48(1):70-74. Epub 2019 Nov 29.

Department of Plant Protection, College of Agriculture, Yangtze University, Jingzhou, China.

is a rare and endangered plant, as well as an endemic species in China. In July 2018, leaf spot and blight disease was observed on . in Yichang, Hubei, China. A fungus isolated from disease tissues was identified as based on morphology and sequence analyses of ITS and LSU regions. Phylogenetic analyses indicated that the species belongs to Dothioraceae (Dothideales). Morphologically, the species produced two distinct types of conidia from authentic media, both conidia were described here. Pathogenicity tests showed that the fungus is a pathogen causing leaf spots on . . This is the first report of leaf spot and blight disease on . caused by . in China.
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http://dx.doi.org/10.1080/12298093.2019.1690405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048172PMC
November 2019

Dynamics of two-dimensional flow around a circular cylinder with flexible filaments attached.

Phys Rev E 2019 Nov;100(5-1):053107

School of Aeronautics and Astronautics, Zhejiang University, Hangzhou 310027, People's Republic of China.

A direction adaptive approach for the reduction of drag and the suppression of lift fluctuation in flow passing a circular cylinder is developed. Flexible filaments are attached to the surface of the cylinder, and different configurations, including the number, lengths, and angles of attachment of the filaments, as well as their tension and bending features, are investigated. In this comprehensive numerical study, the configuration with two filaments 180^{o} apart is found to be optimal for drag reduction and lift fluctuation suppression and is adaptive to the direction of the incoming flow. A drag reduction of 10.8% and a lift fluctuation suppression of 34.6% can be achieved as one filament is attached to the rear stagnation point and the other to the front stagnation point. The hairy coating resembled by 12 evenly attached filaments is also considered. Though marked drag reduction has not been found for this configuration, we leave it an open question for future studies to explore various properties of the filaments in turbulent flow, whose interaction with the filaments would be significant.
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http://dx.doi.org/10.1103/PhysRevE.100.053107DOI Listing
November 2019

Is inferior vena cava compression an alternative for valsalva maneuver in contrast-enhanced transcranial doppler?.

Echocardiography 2020 02 1;37(2):331-336. Epub 2019 Dec 1.

Department of Neurology, Shenzhen People's Hospital, The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University, Shenzhen, China.

Objectives: To evaluate whether inferior vena cava compression (IVCC) can be an alternative for valsalva maneuver (VM) in contrast-enhanced transcranial doppler (c-TCD).

Methods: Patients diagnosed with ischemic stroke, transient ischemic attack, and migraine were enrolled in this study. C-TCD was conducted at resting state, after VM and IVCC to detect right to left shunt (RLS). Then, the RLS was compared to examine whether IVCC could be an alternative for VM in c-TCD.

Results: A total of 246 patients were enrolled in this study. Via Wilcoxon signed-rank test of paired data, the detection for RLS of c-TCD conducted after IVCC was superior to at resting state, but inferior to after VM (P < .001, P = .01, respectively); the detection for RLS of c-TCD conducted after IVCC was inferior to after VM for patients with good cooperation of VM, but was superior for patients with poor cooperation of VM (P < .001, P < .001, respectively); the detection for RLS of c-TCD conducted after IVCC and after VM showed no significant difference for patients with good cooperation of VM and without abdominal obesity, or with poor cooperation of VM and with abdominal obesity (P = .201, P = .157, respectively); the detection for RLS of c-TCD conducted after IVCC was superior to at resting state for patients with abdominal obesity (P < .001).

Conclusions: For patients with poor cooperation of VM, IVCC could be used as an effective supplement to increase the detection of RLS in c-TCD.
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http://dx.doi.org/10.1111/echo.14550DOI Listing
February 2020

Roles of the mammalian target of rapamycin (mTOR) signaling pathway in the repair of hyperoxia-induced acute lung injury.

Adv Clin Exp Med 2020 Jan;29(1):13-23

Neonatal Intensive Care Unit, Women and Children Health Institute Futian, University of South China, Shenzhen, China.

Background: Rapamycin inhibits the mammalian target of rapamycin (mTOR) activity and has been proven effective for the treatment of lung injury.

Objectives: The objective of this study was to investigate the roles of the mTOR pathway and its inhibitor rapamycin in the repair of hyperoxia-induced acute lung injury (ALI).

Material And Methods: Firstly, premature rat lung fibroblast L929 cells were cultured under different oxygen concentrations (40%, 60%, and 90%). At day 3, 7 and 14 after exposure, MTT assay and flow cytometry were used to evaluate the effect of oxygen stress on cell viability and apoptosis of L929 cells, respectively. Secondly, microscopy, MTT assay and flow cytometry was used to investigate the effect of 10 nM rapamycin on 90% O2 exposed L929 cells. We also used small interfering RNAs (siRNAs) to abrogate the expression of mTOR in 90% O2 exposed L929 cells, and then evaluated the apoptosis and cell viability using flow cytometry and the MTT assay, respectively. In addition, western blot was used to detect the protein expression of Bcl-2, p53, TGF-β and connective tissue growth factor (CTGF). A hyperoxia-induced lung injury model was established in Sprague Dawley (SD) rats in order to evaluate the histopathological changes in lung tissues and expression of the mTOR pathway and fibrosis related factors.

Results: Exposure to 40%, 60% or 90% oxygen all significantly inhibited the growth of L929 cells. Application of 10 nM rapamycin was found to effectively promote apoptosis of 90% O2 exposed L929 cells. In addition, mTOR siRNA promoted the apoptosis and inhibited the growth of L929 cells. Rapamycin inhibited the activation of the mTOR signaling pathway, down-regulated the expression of downstream proteins p70S6K and 4EBP1, reduced the collagen deposition and the production of fibrosis-inducing factors, including TGF-β and CTGF in hyperoxia-induced lung injury rats.

Conclusions: Rapamycin may be useful for the treatment of hyperoxia-induced acute lung injury (ALI) by inhibiting the activation of mTOR signaling pathway.
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http://dx.doi.org/10.17219/acem/76170DOI Listing
January 2020