Publications by authors named "Jialing Chen"

19 Publications

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Gasification biochar from horticultural waste: An exemplar of the circular economy in Singapore.

Sci Total Environ 2021 Mar 19;781:146573. Epub 2021 Mar 19.

Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117585, Singapore. Electronic address:

Organic waste, the predominant component of global solid waste, has never been higher, resulting in increased landfilling, incineration, and open dumping that releases greenhouse gases and toxins that contribute to global warming and environmental pollution. The need to create and adopt sustainable closed-loop systems for waste reduction and valorization is critical. Using organic waste as a feedstock, gasification and pyrolysis systems can produce biooil, syngas, and thermal energy, while reducing waste mass by as much as 85-95% through conversion into biochar, a valuable byproduct with myriad uses from soil conditioning to bioremediation and carbon sequestration. Here, we present a novel case study detailing the circular economy of gasification biochar in Singapore's Gardens by the Bay. Biochar produced from horticultural waste within the Gardens was tested as a partial peat moss substitute in growing lettuce, pak choi, and pansy, and found to be a viable substitute for peat moss. At low percentages of 20-30% gasification biochar, fresh weight yields for lettuce and pak choi were comparable to or exceeded those of plants grown in pure peat moss. The biochar was also analyzed as a potential additive to concrete, with a 2% biochar mortar compound found to be of suitable strength for non-structural functions, such as sidewalks, ditches, and other civil applications. These results demonstrate the global potential of circular economies based on local biochar creation and on-site use through the valorization of horticultural waste via gasification, generating clean, renewable heat or electricity, and producing a carbon-neutral to -negative byproduct in the form of biochar. They also indicate the potential of scaled-up pyrolysis or gasification systems for a circular economy in waste management.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146573DOI Listing
March 2021

High-Sensitive Detection of Small-Cell Lung Cancer Cells Based on Terminal Deoxynucleotidyl Transferase-Mediated Extension Polymerization Aptamer Probe.

ACS Biomater Sci Eng 2021 03 5;7(3):1169-1180. Epub 2021 Feb 5.

Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.

Small-cell lung cancer (SCLC) is characterized by early metastasis and high invasiveness, poor prognosis, and a low five-year survival rate. Therefore, the development of the effective detection of SCLC cells and imaging methods has potential significance for the prognosis and treatment of SCLC. We designed a terminal deoxynucleotidyl transferase (TdT)-mediated extension polymerization aptamer probe (denoted as TEPAP). Aptamer HCC03 was used as an element of recognizing SCLC, and it was extended as a long poly(T) tail at the 3'-hydroxyl terminus by TdT and then hybridized with short poly(A) labeled with 6-carboxyfluorescein (FAM) to construct TEPAP for the high-sensitivity detection of SCLC. The results showed that the probe could specifically recognize NCI-H446 cells. Compared with HCC03 labeled with FAM, TEPAP has demonstrated a higher fluorescence signal in recognizing NCI-H446 cells, and the fluorescence intensity of TEPAP recognizing the target cells was 10 times higher than that of nontarget cells. Flow cytometric analysis showed that the detection limit of this method was as low as 17 NCI-H446 cells in 200 μL of binding buffer. In the application of clinical cytology cell blocks, the sensitivity, specificity, and accuracy of TEPAP were 89.74, 94.44, and 91.23%, respectively. The high sensitivity and specificity of TEPAP in the application of clinical samples show that the proposed probe has great potential in the diagnosis of SCLC.
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http://dx.doi.org/10.1021/acsbiomaterials.0c01633DOI Listing
March 2021

Vitiligo-like depigmentation associated with Xuesaitong capsule treatment.

Australas J Dermatol 2020 Dec 18. Epub 2020 Dec 18.

Department of Dermatology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China.

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http://dx.doi.org/10.1111/ajd.13507DOI Listing
December 2020

Quality of internet videos related to exercise therapy of ankylosing spondylitis from mainland China : Content analysis.

Z Rheumatol 2020 Dec 1. Epub 2020 Dec 1.

Department of Computer Technology, Affiliated Hospital of Nantong University, Nantong, China.

Objective: The aim of this study is to assess the quality and reliability of online videos on ankylosing spondylitis (AS) exercises from the five most popular video websites in China.

Design: Cross-sectional and descriptive study.

Methods: We searched the video websites of Youku, Tencent, Tudou, IQiYi, and bilibili on February 15, 2020, using the keywords "Ankylosing spondylitis exercise" "Ankylosing spondylitis rehabilitation" and "Ankylosing spondylitis therapy" A total of 114 videos were included in the study and evaluated according to the Global Quality Scale (GQS) and modified DISCERN tool.

Results: According to the GQS, the videos were classified as high quality (12.3%, n = 14), intermediate quality (63.2%, n = 72), and low quality (24.6%, n = 28). Using the modified DISCERN tool, the videos were divided into useless (53.5%, n = 61), useful (35.1%, n = 40), and misleading (11.4%, n = 13).

Conclusion: The analysis shows that the quality and reliability of online videos related to exercise therapy for Ankylosing spondylitis (AS) should be improved and supervised in China. Hospitals, universities, and medical doctors should make more useful and high-quality videos to provide effective exercise guidance for AS patients.
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http://dx.doi.org/10.1007/s00393-020-00934-9DOI Listing
December 2020

Catalytic Performances of Cu/MCM-22 Zeolites with Different Cu Loadings in NH-SCR.

Nanomaterials (Basel) 2020 Oct 30;10(11). Epub 2020 Oct 30.

Key Laboratory of Hubei Province for Coal Conversion and New Carbon Materials, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, China.

The NH-SCR activities and hydrothermal stabilities of five Cu/MCM-22 zeolites with different Cu loadings ( = 2-10 wt%) prepared by incipient wetness impregnation method were systematically investigated. The physicochemical properties of Cu/MCM-22 zeolites were analyzed by XRD, nitrogen physisorption, ICP-AES, SEM, NH-TPD, UV-vis, H-TPR and XPS experiments. The Cu species existing in Cu/MCM-22 are mainly isolated Cu, CuO and unreducible copper species. The concentrations of both isolated Cu and CuO species in Cu/MCM-22 increase with Cu contents, but the increment of CuO species is more distinct, especially in high Cu loadings (>4 wt%). NH-SCR experimental results demonstrated that the activity of Cu/MCM-22 is sensitive to Cu content at low Cu loadings (≤4 wt%). When the Cu loading exceeds 4 wt%, the NH-SCR activity of Cu/MCM-22 is irrelevant to Cu content due to the severe pore blockage effects caused by aggregated CuO species. Among the five Cu/MCM-22 zeolites, 4Cu/MCM-22 with moderate Cu content has the best NH-SCR performance, which displays higher than 80% NO conversions in a wide temperature window (160-430 °C). Furthermore, the hydrothermal aging experiments (Cu/MCM-22 was treated at 750 °C for 10 h under 10% water vapor atmosphere) illustrated that all the Cu/MCM-22 zeolites exhibit high hydrothermal stability in NH-SCR reactions.
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http://dx.doi.org/10.3390/nano10112170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694057PMC
October 2020

Role of Slit2 upregulation in recurrent miscarriage through regulation of stromal decidualization.

Placenta 2021 Jan 9;103:1-9. Epub 2020 Oct 9.

Department of Pathology, Jinan University School of Medicine, Guangzhou, 510632, China. Electronic address:

Introduction: Knockout mouse model has shown a relationship between Slit2/Robo1 signalling and altered fertility. Altered expression by endometrial epithelium and trophoblast and is associated with the pathogenesis of pregnancy complications but few studies have investigated the expression of decidual Slit2 in miscarriage.

Methods: Expression profiles of Slit2 and Robo1 were measured in human endometrial tissues during the menstrual cycle phases (n = 30), in decidua tissues from recurrent miscarriage (n = 20) and healthy control (n = 20) at 6-8 weeks of gestation. The hormonal regulation of Slit2/Robo1 expression and the role of Slit2/Robo1 signalling in decidualization was investigated in vitro, along with its effects on β-catenin and MET expression.

Results: In human endometrium, Slit2 and Robo1 protein expression in stromal cells were decreased between the late-proliferative and early-secretory phase. In recurrent miscarriage patients, decidual expression Slit2 was increased and associated with lower expression of E-cadherin and higher level vimentin compared to controls. In vitro, the expression of Slit2 was downregulated by cAMP and progesterone in hESCs. Upregulation of Slit2 resulted in inhibition of cell decidualization and β-catenin translocation to nucleus.

Discussion: This study indicates a functional role for Slit2 in endometrial stromal cell decidualization and the pathogenesis of recurrent miscarriage. Aberrant Increase in Slit2 expression may impairs decidualization of endometrial stromal cells leading to recurrent in recurrent miscarriage.
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http://dx.doi.org/10.1016/j.placenta.2020.10.008DOI Listing
January 2021

The Application of Biomass-Based Catalytic Materials in the Synthesis of Cyclic Carbonates from CO and Epoxides.

Molecules 2020 Aug 10;25(16). Epub 2020 Aug 10.

Key Laboratory of Hubei Province for Coal Conversion and New Carbon Materials, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, China.

The synthesis of cyclic carbonates from carbon dioxide (CO) and epoxides is a 100% atom economical reaction and an attractive pathway for CO utilisation. Because CO is a thermodynamically stable molecule, the use of catalysts is mandatory in reducing the activation energy of the CO conversion. Considering environmental compatibility and the high-efficiency catalytic conversion of CO, there is the strong need to develop green catalysts. Biomass-based catalysts, a type of renewable resource, have attracted considerable attention due to their unique properties-non-toxic, low-cost, pollution-free, etc. In this review, recent advances in the development of biomass-based catalysts for the synthesis of cyclic carbonates by CO and epoxides coupling are summarized and discussed in detail. The effect of biomass-based catalysts, functional groups, reaction conditions, and co-catalysts on the catalytic efficiency and selectivity of synthesizing cyclic carbonates process is discussed. We intend to provide a comprehensive understanding of recent experimental and theoretical progress of CO and epoxides coupling reaction and pave the way for both CO conversion and biomass unitization.
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http://dx.doi.org/10.3390/molecules25163627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464904PMC
August 2020

Effects of exercise programmes on pain, disease activity and function in ankylosing spondylitis: A meta-analysis of randomized controlled trials.

Eur J Clin Invest 2020 Dec 11;50(12):e13352. Epub 2020 Aug 11.

Department of General Surgery, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.

Background: To evaluate the effects of exercise programmes on pain, function and disease activity in patients with ankylosing spondylitis (AS).

Materials And Methods: We searched PubMed, Embase, CNKI and Wanfang from inception to February 2020. Randomized controlled trials comparing exercises with nonexercise interventions in AS patients were applied. Studies that assessed the visual analogue scale (VAS) pain score, the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), the Bath Ankylosing Spondylitis Functional Index (BASFI), C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were included. Outcomes of home-based exercise intervention were also reported independently.

Results: Ten studies met the inclusion criteria in all, including 534 patients (278 exercise, 256 control). Compared with the nonexercise group, exercise group had statistically significant improvements in pain (weighted mean difference [WMD]: -1.02 [95% CI: -1.50 to -0.55]) (I : 31%, P <.0001), BASDAI (WMD: -0.85 [95% CI: -1.09 to -0.61]) (I : 20%, P <.00001), and BASFI (WMD: -0.66 [95% CI: -0.95 to -0.38]) (I : 0%, P <.00001), but not in CRP and ESR. What's more, home-based exercise programmes had positive impacts on BASFI, BASDAI and pain.

Conclusions: For patients with AS, exercise programmes improve pain, function and disease activity. To confirm the results, more well-designed randomized controlled trials with large number of patients are required.
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http://dx.doi.org/10.1111/eci.13352DOI Listing
December 2020

Schisandrin B attenuates renal fibrosis via miR-30e-mediated inhibition of EMT.

Toxicol Appl Pharmacol 2019 12 5;385:114769. Epub 2019 Nov 5.

Department of Pathology, School of Medicine, Jinan University, Guangzhou 510632, China. Electronic address:

Tubulointerstitial fibrosis (TIF) is the main pathologic feature of end-stage renal disease. Epithelial-mesenchymal transition (EMT) of proximal tubular cells (PTCs) is one of the most significant features of TIF. MicroRNAs play critical roles during EMT in TIF. However, whether miRNAs can be used as therapeutic targets in TIF therapy remains undetermined. We found that miR-30e, a member of the miR-30 family, is deregulated in TGF-β1-induced PTCs, TIF mice and human fibrotic kidney tissues. Moreover, transcription factors that induce EMT, such as snail, slug, and Zeb2, were direct targets of miR-30e. Using a cell-based miR-30e promoter luciferase reporter system, Schisandrin B (Sch B) was selected for the enhancement of miR-30e transcriptional activity. Our results indicate that Sch B can decrease the expression of snail, slug, and Zeb2, thereby attenuating the EMT of PTCs during TIF by upregulating miR-30e, both in vivo and in vitro. This study shows that miR-30e can serve as a therapeutic target in the treatment of patients with TIF and that Sch B may potentially be used in therapy against renal fibrosis.
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http://dx.doi.org/10.1016/j.taap.2019.114769DOI Listing
December 2019

Fatty infiltration of paraspinal muscles is associated with bone mineral density of the lumbar spine.

Arch Osteoporos 2019 10 15;14(1):99. Epub 2019 Oct 15.

Department of Radiology, Stony Brook Medicine, Stony Brook, NY, USA.

A total of 88 subjects were enrolled to investigate the relationship between paraspinal muscle fatty infiltration and lumbar bone mineral density (BMD) using chemical shift encoding-based water-fat MRI and quantitative computed tomography (QCT), respectively. A moderate inverse correlation between paraspinal muscle proton density fat fraction and lumbar QCT-BMD was found with age, sex, and BMI controlled.

Purpose: To investigate the relationship between paraspinal muscle fatty infiltration and lumbar bone mineral density (BMD).

Methods: A total of 88 subjects were enrolled in this study (52 females, 36 males; age, 46.6 ± 14.2 years old; BMI, 23.2 ± 3.49 kg/m). Proton density fat fractions (PDFF) of paraspinal muscles (erector spinae, multifidus, and psoas) were measured at L2/3, L3/4, and L4/5 levels using chemical shift encoding-based water-fat MRI. Quantitative computed tomography (QCT) was used to assess BMD of L1, L2, and L3. The differences in paraspinal muscle PDFF among subjects with normal bone density, osteopenia, and osteoporosis were tested using one-way ANOVA. The relationship between paraspinal muscle PDFF and QCT-BMD was analyzed using linear regression with age, sex, and BMI variables.

Results: PDFF of the erector spinae, multifidus, and psoas of subjects with normal bone density were all significantly less than those with osteopenia and those with osteoporosis (all p < 0.001). There was an inverse correlation between paraspinal muscle PDFF and BMD after controlling for age, sex, and BMI (standardized beta coefficient, - 0.21~- 0.29; all p < 0.05).

Conclusions: Paraspinal muscle fatty infiltration increased while lumbar BMD decreased after adjusting for age, sex, and BMI. Paraspinal muscles and vertebrae are interacting tissues. Paraspinal muscle fatty infiltration may be a marker of low lumbar BMD. Chemical shift imaging is an efficient and fast quantitative method and can be easily added to the clinical protocol to measure paraspinal muscle PDFF when the patient underwent the routine lumbar MRI with low-back pain.
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http://dx.doi.org/10.1007/s11657-019-0639-5DOI Listing
October 2019

Intravoxel incoherent motion MR imaging for differentiating malignant lesions in spine: A pilot study.

Eur J Radiol 2019 Nov 17;120:108672. Epub 2019 Sep 17.

Università degli Studi di Foggia, Scuola di Specializzazione di Area Medica, Department of Radiology, Foggia, Italy.

Purpose: To determine the diagnostic potential of Intravoxel Incoherent Motion (IVIM) MRI for differentiating malignant spinal tumours from acute vertebral compression fractures and tuberculous spondylitis, and to compare IVIM with diffusion-weighted imaging (DWI) and chemical shift imaging (CSI).

Methods: The Institutional Review Board approved this prospective study, and informed consent was obtained. IVIM MRI, DWI, and CSI at 1.5 T were performed in 25 patients with 12 acute compression fractures, 14 tuberculous spondylitis, and 18 malignant spinal tumours. The parameters of these techniques were assessed using the Kruskal-Wallis test. The diagnostic performance of the parameters was evaluated using receiver operating characteristic (ROC) analysis.

Results: ADC, SIR, D, D, and f values of malignant tumours were significantly different from those of acute compression fracture (for all, p < 0.05). The mean D and D values of malignant spinal tumours had significant differences compared with those of tuberculous spondylitis (for all, p < 0.05). However, no significant differences were observed in any quantitative parameters between the acute compression fracture and the tuberculous spondylitis (p > 0.05). D•f showed the highest AUC value of 0.980 (95%CI: 0.942-1.000) in differentiating acute compression fracture and malignant spinal tumours. D showed the highest AUC value of 0.877 (95%CI: 0.713-0.966) in differentiating tuberculous spondylitis and malignant spinal tumours.

Conclusions: IVIM MR imaging may be helpful for differentiating malignant spinal tumours from acute vertebral compression fractures and tuberculous spondylitis.
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http://dx.doi.org/10.1016/j.ejrad.2019.108672DOI Listing
November 2019

Reproducibility of quantitative susceptibility mapping in lumbar vertebra.

Quant Imaging Med Surg 2019 Apr;9(4):691-699

Department of Medical Imaging, The Third Affiliated Hospital of Southern Medical University (Academy of Orthopedics·Guangdong Province), Guangzhou 510630, China.

Background: To evaluate the reliability and reproducibility of quantitative susceptibility mapping (QSM) in the lumbar vertebra.

Methods: From May 2017 to September 2017, 61 subjects who underwent QSM MRI and quantitative computed tomography (QCT) were consecutively enrolled in this prospective study. QSM examination was performed two times with an interval of less than 1 week for each subject. For each data set, the QSM and QCT values on L1-L4 vertebral bodies were measured independently by two radiologists. The correlation coefficient between QSM and QCT values was calculated on L1-L4 vertebral bodies. The intraclass correlation coefficient (ICC) and Bland-Altman plots were used to evaluate the inter-observer reliability and the inter-scan reproducibility on QSM.

Results: A total of 61 subjects (mean age, 55.5±13.7 years) with 244 vertebral bodies were analyzed. Overall, QSM and QCT showed good correlation in the L1-L4 vertebral body, especially in the L3 (R=-0.75). QSM value showed excellent inter-observer reliability (ICC, 0.992, 95% CI: 0.985-0.996) with a mean difference of 0.35 and 95% limits of agreements of within -22.74 to 23.45 ppb, and very good inter-scan reproducibility (ICC, 0.932, 95% CI: 0.886-0.959) with a mean difference of -7.60 ppb and 95% limits of agreements of within of -92.85 to 77.62 ppb.

Conclusions: QSM in the lumbar vertebra is a reliable and reproducible technique for evaluating bone mineral density.
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http://dx.doi.org/10.21037/qims.2019.04.12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6511713PMC
April 2019

Novel positioning from obesity to cancer: FTO, an mA RNA demethylase, regulates tumour progression.

Authors:
JiaLing Chen Bin Du

J Cancer Res Clin Oncol 2019 Jan 21;145(1):19-29. Epub 2018 Nov 21.

Department of Pathology, School of Medicine, Jinan University, Guangzhou, China.

Purpose: The fat mass- and obesity-associated (FTO) gene on chromosome 16q12.2 shows an intimate association with obesity and body mass index. Recently, research into the FTO gene and its expression product has attracted widespread interest due to the identification of FTO as an N6-methyladenosine (m6A) demethylase. FTO primarily regulates the m6A levels of downstream targets via their 3' untranslated regions. FTO not only plays a critical role in obesity-related diseases but also is involved in the occurrence, development and prognosis of many types of cancer, such as acute myeloid leukaemia, glioblastoma and breast cancer. Currently, studies indicate that FTO is a crucial component of m6A modification, it regulates cancer stem cell function, and promotes the growth, self-renewal and metastasis of cancer cells. In this review, we summarized and analysed the data regarding the structural features and biological functions of FTO as well as its association with different cancers and possible molecular mechanisms.

Methods: We systematically reviewed the related literatures regarding FTO and its demethylation activity in many pathologic and physiological processes, especially in cancer-related diseases based on PubMed databases in this article.

Results: Mounting evidence indicated that FTO plays a critical role in occurrence, progression and treatment of various cancers, even acting as a cancer oncogene in acute myeloid leukaemia, research on which is no longer restricted to metabolic diseases such as obesity and diabetes.

Conclusion: Considering FTO's critical role in many diseases, FTO may become a new promising target for the diagnosis and treatment of various diseases in the near future, especially for specific types of cancers, such as acute myeloid leukaemia, glioblastoma and breast cancer.
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http://dx.doi.org/10.1007/s00432-018-2796-0DOI Listing
January 2019

Chemiluminescence immunoassay for sensing lipoprotein-associated phospholipase A2 in cardiovascular risk evaluation.

Clin Chim Acta 2019 Jan 7;488:143-149. Epub 2018 Nov 7.

Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen 518060, China. Electronic address:

Background: Lipoprotein-associated phospholipase A2 (Lp-PLA) is a novel inflammatory biomarker, which is useful as an adjunct identification tool for cardiovascular disease. However, the important limitation of the conventional enzyme-linked immunosorbent assay (PLAC ELISA) for Lp-PLA assay is its relatively low sensitivity and time consuming. A method to measure the Lp-PLA simply, rapidly and sensitively is essential for predicting cardiovascular events in clinic.

Methods: We took advantage of magnetic separation integrated with chemiluminescence to detect Lp-PLA. The concentration of Lp-PLA was measured through a one-step process by mixing antibody labelled magnetic beads, antigen and antibody at one time.

Results: Our method realized the sample to answer within 17 min. The detection limit and measurement range were 0.18 ng/ml and 0.18-1350 ng/ml, respectively. The specificity assay showed that no appreciable interference was observed for the substances of bilirubin, triglyceride, hemoglobin, rheumatoid factor and human anti-mouse antibody up to the concentrations of 40 mg/dl, 1000 mg/dl, 2000 mg/dl, 1500 IU/ml and 30 ng/ml, separately. We also tested 122 clinical samples using our method, presenting good overall correlations (R = 0.979) to the PLAC ELISA. It is worth mentioning that, our method was faster, had a wider range of measurement and higher sensitivity compared with the PLAC ELISA.

Conclusions: The Lp-PLA assay is straightforward, sensitive and precise, which is highly suitable to further explore the clinical performance of Lp-PLA in studies of cardiovascular risk management.
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http://dx.doi.org/10.1016/j.cca.2018.11.013DOI Listing
January 2019

A letter on a hand: Rotating together or separately?

Q J Exp Psychol (Hove) 2019 Mar 21;72(3):472-480. Epub 2018 Feb 21.

2 Department of Psychology, Oklahoma City University, Oklahoma City, OK, USA.

The motor system plays a role in some object mental rotation tasks, and researchers have reported that people may use a strategy of motor simulation to mentally rotate objects. In this study, we used images of a hand with a letter printed on the palm to directly determine whether a hand image can be automatically rotated during the deliberate mental rotation of an object and whether the hand and object are rotated in the same trajectory. A total of 41 participants were shown the stimuli and asked to decide whether the letters, which were upright or tilted at specific degrees, were normal or mirrored. The hand images in the background showed either a left or a right hand in the palm view, with fingers pointing upwards, medial, downwards, or lateral. Reaction times and error rates were measured to determine the speed and accuracy of mental rotation. A complex interaction between the hand posture and letter orientation revealed that the hand image was mentally rotated automatically, together with the deliberate mental rotation of the letter. The biomechanical constraints of the hand also influenced reaction times, suggesting the involvement of the motor system in the concomitant mental rotation of the hand image. Consistent with the motor simulation theory, the participants seemed to imagine the hand carrying the object in its movement. These behavioural data support the motor simulation theory and elucidate specific processes of mental rotation that have not been addressed by neuroimaging studies.
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http://dx.doi.org/10.1177/1747021818758264DOI Listing
March 2019

Development of pH-responsive polymer and citrate aqueous two-phase system for extractive bioconversion of cefprozil.

Talanta 2017 Nov 12;174:256-264. Epub 2017 May 12.

State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address:

A pH-responsive aqueous two-phase system (pH-ATPS) has been developed by sodium citrate and a recyclable pH-responsive polymer P that can response to the change of pH values. Phase separation mechanism is studied through Low field-NMR. All variables affecting the phase separation are evaluated. Phase characteristics (viscosity, density, interfacial tension) and phase separation kinetic are studied for understanding of separation process and operational parameters in applications. This pH-ATPS has the characters of low interfacial tension, high recovery leading efficient mass transfer and low cost. The proposed system can be used as a mild medium for extractive bioconversion with low cost. We applied this pH-ATPS in extractive bioconversion of cefprozil. Cefprozil is partitioned towards the polymer-rich phase while the substrates tended to be partitioned in the salt-rich phase. Extractive bioconversion of cefprozil in this pH-ATPS can improve yield of the enzymatic process and reduce the product hydrolysis in optimal conditions. The maximal conversion yield of cefprozil in the studied system is 91.0%.
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http://dx.doi.org/10.1016/j.talanta.2017.05.027DOI Listing
November 2017

A Morphological identification cell cytotoxicity assay using cytoplasm-localized fluorescent probe (CLFP) to distinguish living and dead cells.

Biochem Biophys Res Commun 2017 Jan 11;482(2):257-263. Epub 2016 Nov 11.

State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China. Electronic address:

Cell cytotoxicity assays include cell activity assays and morphological identification assays. Currently, all frequently used cytotoxicity assays belong to cell activity assays but suffer from detection limitations. Morphological identification of cell death remains as the gold standard, although the method is difficult to scale up. At present there is no generally accepted morphological identification based cell cytotoxicity assay. In this study, we applied previous developed cell cytoplasm-localized fluorescent probe (CLFP) to display cell morphologies. Under fluorescence microscopy, the fluorescence morphology and intensity of living cells are distinct from dead cells. Based on these characters we extracted the images of living cells from series of samples via computational analysis. Thus, a novel cell morphological identification cytotoxicity assay (CLFP assay) is developed. The performance of the CLFP assay was similar to cell activity assay (MTT assay), but the accuracy of the CLFP assay was superior when measuring the cytotoxicity of active compounds.
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http://dx.doi.org/10.1016/j.bbrc.2016.09.169DOI Listing
January 2017

Glutathione-capped Mn-doped ZnS quantum dots as a room-temperature phosphorescence sensor for the detection of Pb(2+) ions.

Spectrochim Acta A Mol Biomol Spectrosc 2016 07 10;164:98-102. Epub 2016 Apr 10.

State Key Laboratory of Marine Environmental Science of China (Xiamen University), College of Environment and Ecology, Xiamen University, Xiamen 361102, China; Zhangzhou Institute of Technology, Zhangzhou 363000, China. Electronic address:

The room-temperature phosphorescence (RTP) of glutathione-capped Mn-doped ZnS quantum dots (GSH-Mn-ZnS QDs) was effectively quenched by the addition of Pb(2+). A simple and sensitive RTP sensor for Pb(2+) detection based on the quenching effect was developed. Under the optimal conditions, good linear correlations were obtained for Pb(2+) over a concentration range from 1.0 to 100μg·L(-1), and the detection limit was 0.45μg·L(-1). The established method has been successfully applied for the determination of Pb(2+) in real water samples without complicated sample pretreatment with the recoveries in the range of 95.4%-104.0%.
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http://dx.doi.org/10.1016/j.saa.2016.04.014DOI Listing
July 2016

Theoretical insights into the mechanism of olefin elimination in the methanol-to-olefin process over HZSM-5, HMOR, HBEA, and HMCM-22 zeolites.

J Phys Chem A 2014 Oct 3;118(39):8901-10. Epub 2014 Mar 3.

State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences , P.O. Box 165, Taiyuan, Shanxi 030001, PR China.

The mechanism of olefin elimination in the process of methanol-to-olefins (MTO) over a series of zeolites like HZSM-5, HMOR, HBEA, and HMCM-22 was investigated by DFT-D calculations, which is a crucial step that controls the MTO product distribution. The results demonstrate that the manners of olefin elimination are related to the pore structure of zeolite catalyst and the interaction between proton transfer reagent (water or methanol) and zeolite acidic framework. The indirect spiro mechanism is preferable to the direct mechanism over HMOR, HBEA, and HMCM-22 zeolites with large pores, as suggested by the energy barrier of rate-determining step and the potential energy surface (PES), but is unfavorable over HZSM-5 with medium-sized pores due to the steric hindrance of spiro intermediates. Over various zeolites, water and methanol perform differently in proton transfer to form the spiro intermediates; over HMOR and HBEA with strong acidity, water is superior to methanol in promoting propene elimination, whereas over HMCM-22 with relatively weaker acidity, methanol is more favorable as a proton transfer reagent.
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http://dx.doi.org/10.1021/jp5005055DOI Listing
October 2014