Publications by authors named "Jialin Chen"

112 Publications

Regulation of osteogenic differentiation by the pro-inflammatory cytokines IL-1β and TNF-α: current conclusions and controversies.

Hum Cell 2022 May 6. Epub 2022 May 6.

School of Medicine, Southeast University, Nanjing, 210009, China.

Treatment of complex bone fracture diseases is still a complicated problem that is urged to be solved in orthopedics. In bone tissue engineering, the use of mesenchymal stromal/stem cells (MSCs) for tissue repair brings hope to the medical field of bone diseases. MSCs can differentiate into osteoblasts and promote bone regeneration. An increasing number of studies show that the inflammatory microenvironment affects the osteogenic differentiation of MSCs. It is shown that TNF-α and IL-1β play different roles in the osteogenic differentiation of MSCs via different signal pathways. The main factors that affect the role of TNF-α and IL-1β in osteogenic differentiation of MSCs include concentration and the source of stem cells (different species and different tissues). This review in-depth analyzes the roles of pro-inflammatory cytokines in the osteogenic differentiation of MSCs and reveals some current controversies to provide a reference of comprehensively understanding.
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http://dx.doi.org/10.1007/s13577-022-00711-7DOI Listing
May 2022

Multifunctional polyphenol-based silk hydrogel alleviates oxidative stress and enhances endogenous regeneration of osteochondral defects.

Mater Today Bio 2022 Mar 9;14:100251. Epub 2022 Apr 9.

School of Medicine, Southeast University, 210009, Nanjing, China.

In osteochondral defects, oxidative stress caused by elevated levels of reactive oxygen species (ROS) can disrupt the normal endogenous repair process. In this study, a multifunctional hydrogel composed of silk fibroin (SF) and tannic acid (TA), the FDA-approved ingredients, was developed to alleviate oxidative stress and enhance osteochondral regeneration. In this proposed hydrogel, SF first interacts with TA to form a hydrogen-bonded supramolecular structure, which is subsequently enzymatically crosslinked to form a stable hydrogel. Furthermore, TA had multiple phenolic hydroxyl groups that formed interactions with the therapeutic molecule E7 peptide for controlled drug delivery. In vitro investigations showed that SF-TA and SF-TA-E7 hydrogels exhibited a multitude of biological effects including scavenging of ROS, maintaining cell viability, and promoting the proliferation of bone marrow mesenchymal stem cells (BMSCs) against oxidative stress. The proteomic analysis indicated that SF-TA and SF-TA-E7 hydrogels suppressed oxidative stress, which in turn improved cell proliferation in multiple proliferation and apoptosis-related pathways. In rabbit osteochondral defect model, SF-TA and SF-TA-E7 hydrogels promoted enhanced regeneration of both cartilage and subchondral bone as compared to hydrogel without TA incorporation. These findings indicated that the multifunctional SF-TA hydrogel provided a microenvironment suitable for the endogenous regeneration of osteochondral defects.
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http://dx.doi.org/10.1016/j.mtbio.2022.100251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034395PMC
March 2022

Clinical Features of Myasthenia Gravis With Antibodies to MuSK Based on Age at Onset: A Multicenter Retrospective Study in China.

Front Neurol 2022 8;13:879261. Epub 2022 Apr 8.

Department of Neurology, Huashan Hospital, Fudan University, Shanghai, China.

Introduction: Antibodies to MuSK identify a rare subtype of myasthenia gravis (MuSK-MG). In western countries, the onset age of MuSK-MG peaks in the late 30's while it is unknown in Chinese population.

Methods: In this retrospective multicenter study, we screened 69 MuSK-MG patients from 2042 MG patients in five tertiary referral centers in China from October 2016 to October 2021 and summarized the clinical features and treatment outcomes. Then we subgrouped the patients into early-onset (<50 years old), late-onset (50-64 years old), and very-late-onset (≥65 years old) MG and compared the differences in weakness distribution, disease progression and treatment outcomes among three subgroups.

Results: The patients with MuSK-MG were female-dominant (55/69) and their mean age at onset was 44.70 ± 15.84 years old, with a broad range of 17-81 years old. At disease onset, 29/69 patients were classified as MGFA Type IIb and the frequency of bulbar and extraocular involvement was 53.6 and 69.6%, respectively. There was no difference in weakness distribution. Compared with early-onset MuSK-MG, very-late-onset patients had a higher proportion of limb muscle involvement (12/15 vs.16/40, = 0.022) 3 months after onset. Six months after onset, more patients with bulbar (14/15 vs. 26/39, = 0.044) and respiratory involvement (6/15 vs. 0/13, = 0.013) were seen in very-late-onset than in late-onset subgroup. The very-late-onset subgroup had the highest frequency of limb weakness (86.7%, < 0.001). One year after onset, very-late-onset patients demonstrated a higher frequency of respiratory involvement than early-onset patients (4/12 vs. 2/35, = 0.036). 39/64 patients reached MSE. Among 46 patients who received rituximab, very-late-onset patients started earlier than late-onset patients [6 (5.5-7.5) vs. 18 (12-65) months, = 0.039], but no difference in the time and rate to achieving MSE was identified.

Conclusion: MuSK-MG patients usually manifested as acute onset and predominant bulbar and respiratory involvement with female dominance. Very-late-onset patients displayed an early involvement of limb, bulbar and respiratory muscles in the disease course, which might prompt their earlier use of rituximab. The majority MuSK-MG patients can benefit from rituximab treatment regardless of age at onset.
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http://dx.doi.org/10.3389/fneur.2022.879261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9033288PMC
April 2022

Rescue of mis-splicing of a common mutant associated with sensorineural hearing loss by antisense oligonucleotides.

Mol Ther Nucleic Acids 2022 Jun 19;28:280-292. Epub 2022 Mar 19.

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.

A wide spectrum of mutations causes Pendred syndrome and enlarged vestibular aqueduct, both associated with sensorineural hearing loss (SNHL). A splice-site mutation, c.919-2A>G (A-2G), which is common in Asian populations, impairs the 3' splice site of intron 7, resulting in exon 8 skipping during pre-mRNA splicing and a subsequent frameshift that creates a premature termination codon in the following exon. Currently, there is no effective drug treatment for SHNL. For A-2G-triggered SNHL, molecules that correct mis-splicing of the mutant hold promise to treat the disease. Antisense oligonucleotides (ASOs) can promote exon inclusion when targeting specific splicing silencers. Here, we systematically screened a large number of ASOs in a minigene system and identified a few that markedly repressed exon 8 skipping. A lead ASO, which targets a heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 intronic splicing silencer (ISS) in intron 8, promoted efficient exon 8 inclusion in cultured peripheral blood mononuclear cells derived from two homozygous patients. In a partially humanized A-2G mouse model, two subcutaneous injections of the ASO at 160 mg/kg significantly rescued exon 8 splicing in the liver. Our results demonstrate that the ISS-targeting ASO has therapeutic potential to treat genetic hearing loss caused by the A-2G mutation in .
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http://dx.doi.org/10.1016/j.omtn.2022.03.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8987850PMC
June 2022

Therapeutic Effects of Batoclimab in Chinese Patients with Generalized Myasthenia Gravis: A Double-Blinded, Randomized, Placebo-Controlled Phase II Study.

Neurol Ther 2022 Jun 12;11(2):815-834. Epub 2022 Apr 12.

Huashan Rare Disease Center, Department of Neurology, Huashan Hospital, Fudan University, Shanghai, China.

Introduction: We investigated the safety and explore potential efficacy of batoclimab administered subcutaneously in Chinese patients with generalized myasthenia gravis (gMG).

Methods: A randomized, double-blinded, placebo-controlled, parallel phase II study was conducted. First, in the double-blinded treatment period, eligible patients received batoclimab (680 mg), batoclimab (340 mg), or placebo on days 1, 8, 15, 22, 29, and 36. In the open-label treatment period, patients received batoclimab (340 mg) on days 50, 64, and 78. In the follow-up period, patients were examined on days 92, 106, and 120. The primary endpoint was Myasthenia Gravis Activities of Daily Living (MG-ADL) score change on day 43 from baseline.

Results: In total, 30 eligible patients were enrolled, with 11, 10, and 9 patients in the batoclimab 680 mg, batoclimab 340 mg, and placebo groups, respectively. MG-ADL score changes from baseline to day 43 were -2.2 ± 0.9, -4.7 ± 0.6, and -4.4 ± 1.0 in the placebo, batoclimab 340 mg, and 680 mg groups, respectively. Similar changes were observed in Quantitative Myasthenia Gravis, Myasthenia Gravis Composite, and 15-item Myasthenia Gravis Quality of Life scores in the placebo, batoclimab 340 mg, and 680 mg groups, respectively. The proportion of patients with clinically significant improvement on day 43 was higher in the batoclimab groups. On day 120, all four scales in the placebo group had more significant improvement compared with the batoclimab groups, with total serum IgG levels reaching a plateau. No death or treatment-emergent adverse events (TEAEs) led to study discontinuation.

Conclusion: Batoclimab is effective and safe in Chinese patients with gMG.

Trial Registration: This study was registered at ClinicalTrials.gov (NCT04346888) on 15 April 2020, with the first patient enrolled on 23 July 2020.
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http://dx.doi.org/10.1007/s40120-022-00345-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095773PMC
June 2022

Ultrasonic Spray Pyrolysis Synthesis and Photoluminescence of LuAG:Ce Thin Films.

Materials (Basel) 2022 Mar 31;15(7). Epub 2022 Mar 31.

Key Laboratory for Anisotropy and Texture of Materials (Ministry of Education), School of Materials Science and Engineering, Northeastern University, Shenyang 110819, China.

LuAG:Ce (LuAlO:Ce) is one of the most important color converters in white lighting industry. Especially, LuAG:Ce film attracts more attention due to the outstanding advantages, such as the efficient heat dissipation, the saving of rare earth, and so on. Here, LuAG:Ce film on sapphire was successfully prepared by the ultrasonic spray pyrolysis process. The phase, microstructure and photoluminescence of LuAG:Ce films were investigated. LuAG:Ce films had a thickness of around 5 μm, which were well crystallized at 1000 °C in air atmosphere to form the typical garnet structure. Under the protection of CO atmosphere, increasing the annealing temperature greatly enhanced the photoluminescence performance. After annealing at 1500 °C for 5 h in CO atmosphere, 3.0 mol.% Ce doped LuAG:Ce film exhibited the highest emission and excitation intensity. The emission intensity of 3.0 mol.% Ce doped LuAG:Ce film annealed at 1500 °C in CO atmosphere increased up to five times, when compared with the best LuAG:Ce film annealed at 1000 °C in air atmosphere. The effects of Ce doping concentration on the photoluminescence were also examined. As the Ce doping concentration increased from 0.2 mol.% to 7.0 mol.%, the color of LuAG:Ce films changed from yellowish green to greenish yellow. When coupling the 3.0 mol.% Ce doped LuAG:Ce film with a 0.5 W 450 nm blue laser, the formed device successfully emitted white light.
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http://dx.doi.org/10.3390/ma15072577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8999763PMC
March 2022

Quasi-Continuous Network Structure Greatly Improved the Anti-Arc-Erosion Capability of Ag/YO Electrical Contacts.

Materials (Basel) 2022 Mar 26;15(7). Epub 2022 Mar 26.

Foshan Graduate School, Northeastern University, Foshan 528311, China.

Ag/YO has excellent potential to replace Ag/CdO as the environmentally friendly electrical contact material. Using spherical YO as the starting material, Ag/YO contacts with a quasi-continuous network structure were successfully fabricated by a low-energy ball milling treatment. The mean size of YO used ranged from 243 to 980 nm. Due to the differences in the size of YO, Ag/YO contacts had different primitive microstructures, thereby exhibiting distinctive anti-arc-erosion capabilities. Ag/YO contact prepared using 243 nm YO showed the best anti-arc-erosion capability and the most outstanding electrical performance measures, such as low contact resistance, less mass transfer, and no failure up to 10 cycle times. The quasi-continuous network structure formed in the micro-scale was responsible for the excellent electrical performance. The short distance between YO particles in the network promoted the cathode arc motion, and thus alleviated the localized erosion. The results obtained herein may inspire further attempts to design electrical contacts rationally.
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http://dx.doi.org/10.3390/ma15072450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8999512PMC
March 2022

ZNF703 promotes triple-negative breast cancer cells through cell-cycle signaling and associated with poor prognosis.

BMC Cancer 2022 Mar 2;22(1):226. Epub 2022 Mar 2.

Department of Pharmacology, School of Pharmacy, Fujian Provincial Key Laboratory of Natural Medicine Pharmacology, Fujian Medical University, University Town, 1 Xue Yuan Road, 350122, Fuzhou, China.

Background: The oncogenic drivers of triple-negative breast cancer (TNBC), which is characterized by worst prognosis compared with other subtypes, are poorly understood. Although next-generation sequencing technology has facilitated identifying potential targets, few of the findings have been translated into daily clinical practice. The present study is aimed to explore ZNF703 (Zinc finger 703) function and its underlying mechanism in TNBC.

Methods: ZNF703 expressions in tissue microarray were retrospectively examined by immunohistochemistry. The cell proliferation by SRB assay and colony formation assay, as well as cell cycle distribution by flow cytometry were assessed. The protein levels associated with possible underlying molecular mechanisms were evaluated by western blotting. Kaplan-Meier analysis was used to plot survival analysis.

Results: Our data suggest that ZNF703 expressed in 34.2% of triple-negative human breast tumors by immunohistochemistry. In vitro, ZNF703 knockdown had potent inhibitory effects on TNBC cell proliferation and cell cycle, with cyclin D1, CDK4, CDK6, and E2F1 downregulated, while Rb1 upregulated. Moreover, Kaplan-Meier analysis showed that high mRNA expression of ZNF703 was correlated to worse overall survival (HR for high expression was 3.04; 95% CI, 1.22 to 7.57, P = 0.017).

Conclusions: Taken together, the results identified that targeting ZNF703 contributed to the anti-proliferative effects in TNBC cells, due to induced G1-phase arrest. This study is the first to identify ZNF703 as a potentially important protein that is involved in TNBC progression.
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http://dx.doi.org/10.1186/s12885-022-09286-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8889678PMC
March 2022

DCE-MRI Performance in Triple Negative Breast Cancers: Comparison with Non-Triple Negative Breast Cancers.

Curr Med Imaging 2022 Feb 25. Epub 2022 Feb 25.

Department of Breast and Thyroid Surgery, The Second Affiliated Hospital of Chongqing Medical University, No.74, Linjiang Rd, Yuzhong Dist, Chongqing 404100, P.R. China.

Background: Triple negative breast cancers is considered to have the worst prognosis in breast cancer. Dynamic contrast enhanced magnetic resonance imaging has been widely used in the diagnosis of breast cancer because that is more sensitive to breast cancer. However, there are few reports about the MRI characteristics of triple negative breast cancers.

Objective: The aim of the study was to evaluate the imaging finding in triple negative breast cancers compared with non-TNBC and attempt to predict it.

Method: In total, 223 patients with a preoperative diagnosis of breast cancer were enrolled in the study. Dynamic contrast enhanced magnetic resonance imaging was performed before being diagnosed with breast cancer, and histopathological assessment was confirmed after biopsy or operation. The patients were divided into 2 groups based on immunohistochemical, namely the triple negative breast cancers or non-triple negative breast cancers.

Results: The 2 groups demonstrated significant differences regarding the tumor size, margin, outline, burr sign, enhancement, inverted nipple(P<0.05). A multivariate logistic regression analysis was performed to further validate the association of these features, however, only margin [odds ratio (OR), 0.038; 95% confidence interval (CI), 0.014-0.100; <0.001], outline [odds ratio (OR), 0.039; 95% confidence interval (CI), 0.008-0.200; <0.001], burr sign [odds ratio (OR), 2.786; 95% confidence interval (CI), 1.225-6.333; 0.014] and enhancement [odds ratio (OR), 0.131; 95% confidence interval (CI), 0.037-0.457; P=0.001] were associated with TNBC.

Conclusion: The results indicated that the specific dynamic contrast enhanced magnetic resonance imaging features can be possible predictors of pathological results, with a consequent prognostic value.
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http://dx.doi.org/10.2174/1573405618666220225090944DOI Listing
February 2022

Clinical features of gout in adult patients with type Ia glycogen storage disease: a single-centre retrospective study and a review of literature.

Arthritis Res Ther 2022 02 26;24(1):58. Epub 2022 Feb 26.

Department of family medicine & Division of General Internal Medicine, Department of medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, State Key Laboratory of Complex Severe and Rare Diseases (Peking Union Medical College Hospital), Beijing, China.

Background: This study aimed to explore the clinical features of gout in adult patients with glycogen storage disease type Ia (GSD Ia).

Methods: Ninety-five adult patients with GSD Ia admitted to Peking Union Medical College Hospital were retrospectively analysed. A clinical diagnosis of GSD Ia was confirmed in all patients through gene sequencing. All patients had hyperuricaemia; 31 patients complicated with gout were enrolled, and 64 adult GSD Ia patients with asymptomatic hyperuricaemia were selected as a control group during the same period. Clinical characteristics were analysed and compared between the two groups.

Results: Thirty-one of the 95 patients had complications of gout (median age, 25 years; 11 (35.5%) females). All 31 patients had hepatomegaly, abnormal liver function, fasting hypoglycaemia, hyperuricaemia, hyperlipaemia, and hyperlacticaemia. A protuberant abdomen, growth retardation, recurrent epistaxis, and diarrhoea were the most common clinical manifestations. Among these 31 patients, 10 patients (32.3%) had gout as the presenting manifestation and were diagnosed with GSD Ia at a median time of 5 years (range, 1-14) after the first gout flare. The median age of gout onset was 18 years (range, 10-29). Fifteen of the 31 GSD Ia-related gout patients were complicated with gouty tophi, which has an average incidence time of 2 years after the first gouty flare. The mean value of the maximum serum uric acid (SUA) was 800.5 μmol/L (range, 468-1068). The incidence of gout in adult GSD Ia patients was significantly associated with the initial age of regular treatment with raw corn starch, the proportion of urate-lowering therapy initiated during the asymptomatic hyperuricaemic stage, maximum SUA level, and mean cholesterol level.

Conclusions: Determination of GSD Ia should be performed for young-onset gout patients with an early occurrence of gouty tophi, especially in patients with hepatomegaly, recurrent hypoglycaemia, or growth retardation. Early detection and long-term regulatory management of hyperuricaemia, in addition to early raw corn starch and lifestyle intervention, should be emphasized for GSD Ia patients in order to maintain good metabolic control.

Trial Registration: Retrospectively registered.
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http://dx.doi.org/10.1186/s13075-021-02706-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881853PMC
February 2022

Knowledge Mapping of Immunotherapy for Hepatocellular Carcinoma: A Bibliometric Study.

Front Immunol 2022 31;13:815575. Epub 2022 Jan 31.

Hepatobiliary and Pancreatic Center, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Background: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors, and many patients are diagnosed with advanced disease. The treatment of advanced liver cancer has made significant strides in recent years, owing to the practice of immunotherapy drugs. Numerous studies have been published on immunotherapy for HCC; however, no relevant bibliometric study has been published. This study aims to gain a better understanding of the current situation and to identify potential new research directions by conducting a bibliometric analysis on immunotherapy for HCC.

Methods: We searched the Web of Science Core Collection (WoSCC) for articles related to immunotherapy for HCC. Three software (VOSviewer, CiteSpace, and python) were primarily used to assess the contribution and co-occurrence relationships of various countries/regions, institutes, journals, and, authors as well as to identify research hotspots and promising future trends in this research field.

Results: A total of 1,641 English articles published between 2011 and 2020 were collected, with the number of articles increasing nearly every year. The majority of publications originated from China ( = 893, 54.42%), followed by the United States and Japan. The Sun Yat-sen University contributed the most publications ( = 97, 5.91%). Nakatsura Tetsuya ( = 26) and Llovet JM ( = 366) were ranked first in the top ten authors and co-cited authors. was the most productive academic journal on immunotherapy for HCC [ = 46, 2.80%; impact factor (IF) 2020 = 6.9679]. Aggregation and identification of critical nodes in the co-cited network demonstrated a shift in the field of HCC immunotherapy. Initially, the hotspots were predominantly "glypican-3", "cytokine-induced killer cells", and "ny-eso-1", while the emphasis has shifted in recent years to "landscape", "camrelizumab", "combination therapy", and "immune score".

Conclusion: Increased attention has been paid to HCC with the advancement of immunotherapy. At the moment, the most active frontiers are focused on better understanding the immunological landscape of liver cancer, screening the population that can benefit from immunotherapy, and the clinical application of immune checkpoint inhibitors, particularly in combination with other therapeutic options (such as local therapy and targeted therapy).
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http://dx.doi.org/10.3389/fimmu.2022.815575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8841606PMC
March 2022

Separation and Characterization of Cellulose Fibers from Cannabis Bast Using Foamed Nickel by Cathodic Electro-Fenton Oxidation Strategy.

Polymers (Basel) 2022 Jan 19;14(3). Epub 2022 Jan 19.

College of Light Industry and Textile, Qiqihar University, Qiqihar 161000, China.

Degumming is the most important link in the textile industry. The main purpose of degumming is to effectively remove non-cellulose substances in plant bast fibers. In this research, we propose an electro-Fenton (EF) system with a nickel-foam (Ni-F) cathode in weak acid pH (EF/Ni-F) to degum cannabis fiber in EF while reducing the content of pollutants in degumming wastewater. FT-IR, XPS, XRD, SEM, and TG were employed to thoroughly understand the reaction characteristics to characterize chemical components, element qualities, the crystallinity, and the morphologies of degummed fibers. Additionally, physical and mechanical properties such as breaking strength, elongation at breaking, residual glue rate, whiteness, and diameter of degummed fibers were measured. Through testing, it was found that the fiber degummed by the EF method had higher breaking strength, lower residual tackiness, and higher whiteness than other methods. The antibacterial test was used to detect the effect of fiber on Staphylococcus aureus before and after degumming. EF could remove more colloidal components from cannabis than other methods, and the mechanical properties were also enhanced. The characteristics of the degummed fiber further confirmed the effectiveness of the new degumming method. Moreover, the antibacterial experiment found that the antibacterial property of the degummed fiber was enhanced. The colloidal components in the degumming wastewater were flocculated and precipitated. The upper liquid of the solution had low chromaticity, low COD value, and weak acid pH value, which can meet the discharge requirements. The above test proves that EF is an effective degumming method that is environmentally friendly, takes less time, and enhances antibacterial performance.
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http://dx.doi.org/10.3390/polym14030380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838538PMC
January 2022

Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.

Cell Chem Biol 2022 Feb 5. Epub 2022 Feb 5.

Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC 27708, USA; Department of Cancer Biology, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA; Department of Pathology, Duke University School of Medicine, Durham, NC 27710, USA. Electronic address:

Signal transduction pathways post-translationally regulating nucleotide metabolism remain largely unknown. Guanosine monophosphate reductase (GMPR) is a nucleotide metabolism enzyme that decreases GTP pools by converting GMP to IMP. We observed that phosphorylation of GMPR at Tyr267 is critical for its activity and found that this phosphorylation by ephrin receptor tyrosine kinase EPHA4 decreases GTP pools in cell protrusions and levels of GTP-bound RAC1. EPHs possess oncogenic and tumor-suppressor activities, although the mechanisms underlying switches between these two modes are poorly understood. We demonstrated that GMPR plays a key role in EPHA4-mediated RAC1 suppression. This supersedes GMPR-independent activation of RAC1 by EPHA4, resulting in a negative overall effect on melanoma cell invasion and tumorigenicity. Accordingly, EPHA4 levels increase during melanoma progression and inversely correlate with GMPR levels in individual melanoma tumors. Therefore, phosphorylation of GMPR at Tyr267 is a metabolic signal transduction switch controlling GTP biosynthesis and transformed phenotypes.
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http://dx.doi.org/10.1016/j.chembiol.2022.01.007DOI Listing
February 2022

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

J Clin Invest 2022 Apr;132(7)

Department of Neuroscience, Institute of Neurosurgery, and Department of Neurosurgery, The First Affiliated Hospital, State Key Laboratory of Cellular Stress Biology, School of Medicine.

Dysfunction of protein trafficking has been intensively associated with neurological diseases, including neurodegeneration, but whether and how protein transport contributes to oligodendrocyte (OL) maturation and myelin repair in white matter injury remains unclear. ER-to-Golgi trafficking of newly synthesized proteins is mediated by coat protein complex II (COPII). Here, we demonstrate that the COPII component Sec13 was essential for OL differentiation and postnatal myelination. Ablation of Sec13 in the OL lineage prevented OPC differentiation and inhibited myelination and remyelination after demyelinating injury in the central nervous system (CNS), while improving protein trafficking by tauroursodeoxycholic acid (TUDCA) or ectopic expression of COPII components accelerated myelination. COPII components were upregulated in OL lineage cells after demyelinating injury. Loss of Sec13 altered the secretome of OLs and inhibited the secretion of pleiotrophin (PTN), which was found to function as an autocrine factor to promote OL differentiation and myelin repair. These data suggest that Sec13-dependent protein transport is essential for OL differentiation and that Sec13-mediated PTN autocrine signaling is required for proper myelination and remyelination.
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http://dx.doi.org/10.1172/JCI155096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8970680PMC
April 2022

Advances in Regulatory Strategies of Differentiating Stem Cells towards Keratocytes.

Stem Cells Int 2022 31;2022:5403995. Epub 2022 Jan 31.

School of Medicine, Southeast University, 210009 Nanjing, China.

Corneal injury is a commonly encountered clinical problem which led to vision loss and impairment that affects millions of people worldwide. Currently, the available treatment in clinical practice is corneal transplantation, which is limited by the accessibility of donors. Corneal tissue engineering appears to be a promising alternative for corneal repair. However, current experimental strategies of corneal tissue engineering are insufficient due to inadequate differentiation of stem cell into keratocytes and thus cannot be applied in clinical practice. In this review, we aim to clarify the role and effectiveness of both biochemical factors, physical regulation, and the combination of both to induce stem cells to differentiate into keratocytes. We will also propose novel perspectives of differentiation strategy that may help to improve the efficiency of corneal tissue engineering.
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http://dx.doi.org/10.1155/2022/5403995DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8820938PMC
January 2022

Somatic Mutation Profiles Revealed by Next Generation Sequencing (NGS) in 39 Chinese Hepatocellular Carcinoma Patients.

Front Mol Biosci 2021 18;8:800679. Epub 2022 Jan 18.

Hepatobiliary and Pancreatic Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

The features and significance of somatic mutation profiles in hepatocellular carcinoma (HCC) have not been completely elucidated to date. In this study, 39 tumor specimens from HCC patients were collected for gene variation analysis by next-generation sequencing (NGS), and a correlation analysis between mutated genes and clinical characteristics was also conducted. The results were compared with genome data from cBioPortal database. Our study found that T > G/A > C transversions (Tv) and C > T/G > A transitions (Ti) were dominant. The sequence variations of TP53, MUC16, MUC12, MUC4 and others, and the copy number variations (CNVs) of FGF3, TERT, and SOX2 were found to be more frequent in our cohort than in cBioPortal datasets, and they were highly enriched in pathways in cancer and participated in complex biological regulatory processes. The TP53 mutation was the key mutation (76.9%, 30/39), and the most common amino acid alteration and mutation types were p.R249S (23.5%) and missense mutation (82.3%) in the TP53 variation. Furthermore, TP53 had more co-mutations with MUC17, NBPF10, and AHNAK2. However, there were no significant differences in clinical characteristics between HCC patients with mutant TP53 and wild-type TP53, and the overall survival rate between treatment precision medication guided by NGS and that empirical medication (logrank = 0.181). Therefore, the role of NGS in the guidance of personalized targeted therapy, solely based on NGS, may be limited. Multi-center, large sample, prospective studies are needed to further verify these results.
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http://dx.doi.org/10.3389/fmolb.2021.800679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8804344PMC
January 2022

Repeatability and clinical use of pupillary light reflex measurement using RAPDx® pupillometer.

Int Ophthalmol 2022 Jan 17. Epub 2022 Jan 17.

Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, 69# North Dongxia Road, Jinping District, Shantou, 515041, Guangdong, People's Republic of China.

Objective: To evaluate the repeatability of pupillary light reflex metrics measured by the RAPDx® dynamic pupillometer in healthy subjects and clinical application in patients with unilateral optic neuritis (ON).

Methods: Sixty eyes of 30 healthy volunteers were measured three times consecutively by the same technician. The amplitude of constriction (AC), the latency of constriction (LOC), the velocity of peak constriction (VC) of light-evoked pupillary constriction, RAPD score for amplitude and latency were measured using RAPDx® dynamic pupillometer. The repeatability of above metrics was assessed by the intraclass correlation coefficient (ICC) and coefficient of variation (Cov). Furthermore, pupillary light reflex measurements were performed in 48 eyes of 24 patients diagnosed with unilateral optic neuritis (ON). Interocular symmetry was evaluated both in the healthy subjects and the ON-involved patients.

Results: High repeatability of AC, LOC, and VC in healthy subjects was displayed, presenting with the ICC value over 0.80 and the Cov less than 8.00%. But the RAPD score for amplitude (ICC: 0.67) and RAPD score for latency (ICC: 0.65) showed only moderate repeatability. Furthermore, a slight declining trend was found in amplitude and peak velocity when continuous and multiple measurements in the healthy subjects. Good symmetry of the AC, LOC, and VC of pupillary light constriction between the two eyes was displayed in the healthy subjects (P > 0.05). By contrast, there was a distinct decrease of AC and VC (P < 0.01), and a mild increase of LOC (P < 0.01) in the ON-involved eye in direct pupillary light reflex.

Conclusions: Pupillary light reflex measured by the RAPDx® pupillometer achieved overall good repeatability and interocular symmetry in healthy subjects. The device also presented decent preliminary results in patients with unilateral ON, suggesting its potential value to be developed as a tool in optic nerve diseases.
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http://dx.doi.org/10.1007/s10792-022-02222-8DOI Listing
January 2022

Risk Factors, Prognostic Factors, and Nomogram for Distant Metastasis in Breast Cancer Patients Without Lymph Node Metastasis.

Front Endocrinol (Lausanne) 2021 24;12:771226. Epub 2021 Nov 24.

Department of Breast and Thyroid Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: Lymph node negative (N0) breast cancer can be found coexisting with distant metastasis (DM), which might consequently make clinicians underestimate the risk of relapse and insufficient treatment for this subpopulation.

Methods: The clinicopathological characteristics of N0 breast cancer patients from the Surveillance, Epidemiology, and End Results (SEER) database between January 2010 and December 2015 were retrospectively reviewed. Multivariate logistic and Cox analyses were used to identify independent risk factors in promoting DM and the 1-, 3-, and 5- year cancer-specific survival (CSS) in this subpopulation.

Result: Seven factors including age (<40 years), tumor size (>10 mm), race (Black), location (central), grade (poor differentiation), histology (invasive lobular carcinoma), and subtype (luminal B and Her-2 enriched) were associated with DM, and the area under curve (AUC) was 0.776 (95% CI: 0.763-0.790). Moreover, T1-3N0M1 patients with age >60 years at diagnosis, Black race, triple-negative breast cancer subtype, no surgery performed, and multiple DMs presented a worse 1-, 3-, and 5-year CSS. The areas under the ROC for 1-, 3-, and 5- year CSS in the training cohort were 0.772, 0.741, and 0.762, respectively, and 0.725, 0.695, and 0.699 in the validation cohort.

Conclusion: The clinicopathological characteristics associated with the risk of DM and the prognosis of female breast cancer patients without lymph node metastasis but with DM are determined. A novel nomogram for predicting 1-, 3-, 5- year CSS in T1-3N0M1 patients is also well established and validated, which could help clinicians better stratify patients who are at a high-risk level for receiving relatively aggressive management.
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http://dx.doi.org/10.3389/fendo.2021.771226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8653828PMC
February 2022

The application of human periodontal ligament stem cells and biomimetic silk scaffold for in situ tendon regeneration.

Stem Cell Res Ther 2021 12 4;12(1):596. Epub 2021 Dec 4.

School of Medicine, Southeast University, Nanjing, 210009, China.

Background: With the development of tissue engineering, enhanced tendon regeneration could be achieved by exploiting suitable cell types and biomaterials. The accessibility, robust cell amplification ability, superior tendon differentiation potential, and immunomodulatory effects of human periodontal ligament stem cells (hPDLSCs) indicate their potential as ideal seed cells for tendon tissue engineering. Nevertheless, there are currently no reports of using PDLSCs as seed cells. Previous studies have confirmed the potential of silk scaffold for tendon tissue engineering. However, the biomimetic silk scaffold with tendon extracellular matrix (ECM)-like structure has not been systematically studied for in situ tendon regeneration. Therefore, this study aims to evaluate the effects of hPDLSCs and biomimetic silk scaffold on in situ tendon regeneration.

Methods: Human PDLSCs were isolated from extracted wisdom teeth. The differentiation potential of hPDLSCs towards osteo-, chondro-, and adipo-lineage was examined by cultured in different inducing media. Aligned and random silk scaffolds were fabricated by the controlled directional freezing technique. Scaffolds were characterized including surface structure, water contact angle, swelling ratio, degradation speed and mechanical properties. The biocompatibility of silk scaffolds was evaluated by live/dead staining, SEM observation, cell proliferation determination and immunofluorescent staining of deposited collagen type I. Subsequently, hPDLSCs were seeded on the aligned silk scaffold and transplanted into the ruptured rat Achilles tendon. Scaffolds without cells served as control groups. After 4 weeks, histology evaluation was carried out and macrophage polarization was examined to check the repair effects and immunomodulatory effects.

Results: Human PDLSCs were successfully isolated, and their multi-differentiation potential was confirmed. Compared with random scaffold, aligned silk scaffold had more elongated and aligned pores and promoted the proliferation and ordered arrangement of hPDLSCs. After implantation into rat Achilles tendon defect, hPDLSCs seeded aligned silk scaffold enhanced tendon repair with more tendon-like tissue formation after 4 weeks, as compared to the scaffold-only groups. Higher expression of CD206 and lower expression of iNOS, IL-1β and TNF-α were found in the hPDLSCs seeded aligned silk scaffold group, which revealed its modulation effect of macrophage polarization from M1 to M2 phenotype.

Conclusions: In summary, this study demonstrates the efficacy of hPDLSCs as seed cells and aligned silk scaffold as a tendon-mimetic scaffold for enhanced tendon tissue engineering, which may have broad implications for future tendon tissue engineering and regenerative medicine researches.
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http://dx.doi.org/10.1186/s13287-021-02661-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8642874PMC
December 2021

[Effect of graphene oxide on the function of erythrocytes].

Sheng Wu Gong Cheng Xue Bao 2021 Nov;37(11):4047-4055

College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.

The biocompatibility of nanomaterials has attracted much attention. Graphene oxide (GO) is a nanomaterial widely used in biomedicine, but its toxicity can not be ignored. In this study, the effect of GO on the blood system (the hemolysis rate, the fragility of erythrocyte, and acetylcholinesterase activity) was systematically investigated. The results showed that the hemolysis rate of erythrocytes was lower than 8% when the GO concentration was below 100 μg/mL (P<0.01). GO at low concentration levels (<5 μg/mL) had no significant effect on the fragility of erythrocytes, but GO at high concentration (10 μg/mL) increased the fragility of erythrocytes (P=0.01). Moreover, GO increased the activity of acetylcholinesterase on erythrocytes. The concentration of 20 μg/mL graphene oxide with the size >5 μm (LGO) increased the activity of acetylcholinesterase by 42.67% (P<0.05). Then molecular dynamics simulation was used to study how GO interacted with acetylcholinesterase and increased its activity. The results showed that GO was attached to the cell membrane, thus may provide an electronegative environment that helps the hydrolysate to detach from the active sites more quickly so as to enhance the activity of acetylcholinesterase.
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http://dx.doi.org/10.13345/j.cjb.200794DOI Listing
November 2021

Construction of Prognostic Risk Model of 5-Methylcytosine-Related Long Non-Coding RNAs and Evaluation of the Characteristics of Tumor-Infiltrating Immune Cells in Breast Cancer.

Front Genet 2021 29;12:748279. Epub 2021 Oct 29.

Department of Breast Surgery, Quanzhou First Hospital of Fujian Medical University, Quanzhou, China.

The role of 5-methylcytosine-related long non-coding RNAs (m5C-lncRNAs) in breast cancer (BC) remains unclear. Here, we aimed to investigate the prognostic value, gene expression characteristics, and correlation between m5C-lncRNA risk model and tumor immune cell infiltration in BC. The expression matrix of m5C-lncRNAs in BC was obtained from The Cancer Genome Atlas database, and the lncRNAs were analyzed using differential expression analysis as well as univariate and multivariate Cox regression analysis to eventually obtain BC-specific m5C-lncRNAs. A risk model was developed based on three lncRNAs using multivariate Cox regression and the prognostic value, accuracy, as well as reliability were verified. Gene set enrichment analysis (GSEA) was used to analyze the Kyoto Encyclopedia of Genes and Genomes signaling pathway enrichment of the risk model. CIBERSORT algorithm and correlation analysis were used to explore the characteristics of the BC tumor-infiltrating immune cells. Finally, reverse transcription-quantitative polymerase chain reaction was performed to detect the expression level of three lncRNA in clinical samples. A total of 334 differential m5C-lncRNAs were identified, and three BC-specific m5C-lncRNAs were selected, namely AP005131.2, AL121832.2, and LINC01152. Based on these three lncRNAs, a highly reliable and specific risk model was constructed, which was proven to be closely related to the prognosis of patients with BC. Therefore, a nomogram based on the risk score was built to assist clinical decisions. GSEA revealed that the risk model was significantly enriched in metabolism-related pathways and was associated with tumor immune cell infiltration based on the analysis with the CIBERSORT algorithm. The efficient risk model based on m5C-lncRNAs associated with cancer metabolism and tumor immune cell infiltration could predict the survival prognosis of patients, and AP005131.2, AL121832.2, and LINC01152 could be novel biomarkers and therapeutic targets for BC.
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http://dx.doi.org/10.3389/fgene.2021.748279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585929PMC
October 2021

Regulation of local GTP availability controls RAC1 activity and cell invasion.

Nat Commun 2021 10 19;12(1):6091. Epub 2021 Oct 19.

Department of Cancer Biology, Wake Forest University Baptist Medical Center, Winston-Salem, NC, USA.

Physiological changes in GTP levels in live cells have never been considered a regulatory step of RAC1 activation because intracellular GTP concentration (determined by chromatography or mass spectrometry) was shown to be substantially higher than the in vitro RAC1 GTP dissociation constant (RAC1-GTP Kd). Here, by combining genetically encoded GTP biosensors and a RAC1 activity biosensor, we demonstrated that GTP levels fluctuating around RAC1-GTP Kd correlated with changes in RAC1 activity in live cells. Furthermore, RAC1 co-localized in protrusions of invading cells with several guanylate metabolism enzymes, including rate-limiting inosine monophosphate dehydrogenase 2 (IMPDH2), which was partially due to direct RAC1-IMPDH2 interaction. Substitution of endogenous IMPDH2 with IMPDH2 mutants incapable of binding RAC1 did not affect total intracellular GTP levels but suppressed RAC1 activity. Targeting IMPDH2 away from the plasma membrane did not alter total intracellular GTP pools but decreased GTP levels in cell protrusions, RAC1 activity, and cell invasion. These data provide a mechanism of regulation of RAC1 activity by local GTP pools in live cells.
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http://dx.doi.org/10.1038/s41467-021-26324-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8526568PMC
October 2021

Multiple targeted self-emulsifying compound RGO reveals obvious anti-tumor potential in hepatocellular carcinoma.

Mol Ther Oncolytics 2021 Sep 19;22:604-616. Epub 2021 Aug 19.

Key Laboratory of Molecular Oncology and Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Hepatocellular carcinoma (HCC) is a highly vascularized, inflammatory, and abnormally proliferating tumor. Monotherapy is often unable to effectively and comprehensively inhibit the progress of HCC. In present study, we selected ginsenoside Rg3, ganoderma lucidum polysaccharide (GLP), and oridonin as the combined therapy. These three plant monomers play important roles in anti-angiogenesis, immunological activation, and apoptosis promotion, respectively. However, the low solubility and poor bioavailability seriously hinder their clinical application. To resolve these problems, we constructed a new drug, Rg3, GLP, and oridonin self-microemulsifying drug delivery system (RGO-SMEDDS). We found that this drug effectively inhibits the progression of HCC by simultaneously targeting multiple signaling pathways. RGO-SMEDDS restored immune function by suppressing the production of immunosuppressive cytokine and M2-polarized macrophages, reduced angiogenesis by downregulation of vascular endothelial growth factor and its receptor, and retarded proliferation by inhibiting the epidermal growth factor receptor EGFR/AKT/epidermal growth factor receptor/protein kinase B/glycogen synthase kinase-3 (GSK3) signaling pathway. In addition, RGO-SMEDDS showed considerable safety in acute toxicity tests. Results from this study show that RGO-SMEDDS is a promising therapy for the treatment of HCC.
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http://dx.doi.org/10.1016/j.omto.2021.08.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449031PMC
September 2021

Carbon-Based Nanomaterials for Bone and Cartilage Regeneration: A Review.

ACS Biomater Sci Eng 2021 10 29;7(10):4718-4735. Epub 2021 Sep 29.

School of Medicine, Southeast University, 210009 Nanjing, China.

As the main load-bearing structure in the human body, bone and cartilage are susceptible to damage in sports and other activities. The repair and regeneration of bone and articular cartilage have been extensively studied in the past decades. Traditional approaches have been widely applied in clinical practice, but the effect varies from person to person and may cause side effects. With the rapid development of tissue engineering and regenerative medicine, various biomaterials show great potential in the regeneration of bone and cartilage. Carbon-based nanomaterials are solid materials with different structures and properties composed of allotropes of carbon, which are classified into zero-, one-, and two-dimensional ones. This Review systemically summarizes the different types of carbon-based nanomaterials, including zero-dimensional (fullerene, carbon dots, nanodiamonds), one-dimensional (carbon nanotubes), and two-dimensional (graphenic materials) as well as their applications in bone, cartilage, and osteochondral regeneration. Current limitations and future perspectives of carbon-based nanomaterials are also discussed.
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http://dx.doi.org/10.1021/acsbiomaterials.1c00759DOI Listing
October 2021

Microfluidic aqueous two-phase system-based nitrifying bacteria encapsulated colloidosomes for green and sustainable ammonium-nitrogen wastewater treatment.

Bioresour Technol 2021 Dec 24;342:126019. Epub 2021 Sep 24.

School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031, PR China. Electronic address:

A novel strategy was proposed for preparing micro-scale monodisperses nitrifying bacteria (NB) encapsulated [email protected] colloidosomes by exploiting capillary microfluidic device, as an attempt to treat ammonium-nitrogen wastewater in an environment-friendly, efficient and repeatable manner based on the aqueous two-phase (ATPS) system. By complying with the spatial confined urease mediate biomineralization reactions, ATPS droplets (Dextran in Polyethylene glycol) containing urease, NB regent and alginate were used as templates to prepare 500 μm [email protected] colloidosomes with 16.48 Mpa mechanical strength. The activity of NB encapsulated in the colloidosomes was high. The simulated wastewater treated with the colloidosomes achieved a high removal rate even at harsh temperature and pH value. In both simulated and real wastewater treatment, prolonged reuse times (216 h) with high removal rate (>90%, after being applied 72 h) were obtained by using [email protected] colloidosomes, as compared with that (96 h) by using general alginate microbeads.
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http://dx.doi.org/10.1016/j.biortech.2021.126019DOI Listing
December 2021

Development and validation of a population-based model for predicting the regional lymph node metastasis in adolescent differentiated thyroid carcinoma.

Oral Oncol 2021 10 24;121:105507. Epub 2021 Aug 24.

Department of Breast and Thyroid Surgery, The Second Affiliated Hospital of Chongqing Medical University, No.74, Linjiang Rd, Yuzhong Dist, Chongqing 404100, PR China. Electronic address:

Background: Adolescent differentiated thyroid carcinoma (DTC) is a rare type of thyroid cancer that represents a special entity of all endocrine-related cancer. This study aims to establish the first nomogram for predicting the regional (central and lateral) lymph node metastasis (LNM) in the adolescent population for better surgical management.

Method: We retrospectively reviewed the clinicopathology characteristics of adolescent patients with DTC in the Surveillance, Epidemiology, and End Results database between 2010 and 2015.

Results: A total of 1,930 adolescent patients between the ages of 10 and 24 years from the SEER database were enrolled in this study. Six predictive factors including age, race, histology, multifocality, extrathyroidal invasion (EI) and tumor size were identified to be significantly associated with the regional LNM via univariate and multivariate logistic regression analyses. These indicators were used to construct a nomogram for predicting the regional LNM in adolescent patients with DTC. Moreover, a satisfied predictive ability of the model was determined with a C-index of 0.794, supported by an internal validation group with a C-index of 0.776. The Decision Curve Analysis and calibration curve further conducted a great agreement in our model.

Conclusion: The first predictive model containing multiple factors has been successfully established with good discrimination for predicting the regional LNM in adolescent patients with DTC. This nomogram could effectively help surgeons to make better individualized surgical decision intraoperatively, especially in terms of whether cervical lymph node dissection (LND) is warranted.
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http://dx.doi.org/10.1016/j.oraloncology.2021.105507DOI Listing
October 2021

Preoperatively Predicting the Central Lymph Node Metastasis for Papillary Thyroid Cancer Patients With Hashimoto's Thyroiditis.

Front Endocrinol (Lausanne) 2021 22;12:713475. Epub 2021 Jul 22.

Department of Breast and Thyroid Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: The preoperative distinguishment of lymph nodes with reactive hyperplasia or tumor metastasis plays a pivotal role in guiding the surgical extension for papillary thyroid carcinoma (PTC) with Hashimoto's thyroiditis (HT), especially in terms of the central lymph node (CLN) dissection. We aim to identify the preparative risk factors for CLN metastasis in PTC patients concurrent with HT.

Materials And Methods: We retrospectively reviewed and analyzed the data including the basic information, preoperative sonographic characteristics, and thyroid function of consecutive PTC patients with HT in our medical center between Jan 2019 and Apr 2021. The Chi-square and Fisher's exact tests were used for comparison of qualitative variables among patients with or without CLN metastasis. Univariate and multivariate logistic regression analyses were used to determine the risk factors for CLN metastasis. The nomogram was constructed and further evaluated by two cohorts produced by 1,000 resampling bootstrap analysis.

Results: A total of 98 in 214 (45.8%) PTC patients were identified with CLN metastasis. In multivariate analysis, four variables including high serum thyroglobulin antibody (TgAb) level (>1,150 IU/ml), lower tumor location, irregular margin of CLN, and micro-calcification in the CLN were determined to be significantly associated with the CLN metastasis in PTC patients with HT. An individualized nomogram was consequently established with a favorable C-index of 0.815 and verified two internal validation cohorts.

Conclusions: Our results indicated that preoperatively sonographic characteristics of the tumor and lymph node condition combined with serum TgAb level can significantly predict the CLN in PTC patients with HT and the novel nomogram may further help surgeons to manage the CLN in this subpopulation.
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http://dx.doi.org/10.3389/fendo.2021.713475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339927PMC
February 2022

Correlation Between Electroretinogram and Visual Prognosis in Metallic Intraocular Foreign Body Injury.

Front Med (Lausanne) 2021 24;8:688305. Epub 2021 Jun 24.

Joint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, China.

This study aims to investigate the correlation between electroretinogram (ERG) and visual outcome in eyes with metallic intraocular foreign body (IOFB) injury. Cases with metallic IOFB injuries with preoperative ERG from January 2008 to May 2020 were reviewed retrospectively. Five ERG responses were recorded, including rod response, maximal response, oscillatory potentials, cone response, and 30-Hz flicker. The results were compared between the affected and the contralateral eyes. All patients received surgery to remove IOFBs. The correlation between amplitudes, implicit times, and grades of ERG with final best-corrected visual acuity (BCVA) was analyzed. A total of 33 eyes of 33 patients were included. The eyes with IOFB had generally delayed implicit time and reduced amplitude in all waves. The maximum change was found in oscillatory potentials S3 and N1 (0.42 ± 0.42 and 1.95 ± 1.97 of the fellow eyes, respectively, < 0.05). All amplitudes were negatively correlated with the final BCVA (rs: -0.676 to -0.459, all < 0.05). In contrast, all implicit times were positively correlated with final BCVA, although, some of them were not statistically significant (rs: 0.035 to 0.687). Among them, oscillatory potential P3 has the highest correlation coefficient (rs = 0.687, < 0.001). All grades of ERG waves were statistically correlated with the final BCVA (rs: -0.596 to -0.664, all < 0.001). ERG can be used to assess visual outcome in metallic IOFB injury after surgery. Oscillatory potentials provided the most significant responses.
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http://dx.doi.org/10.3389/fmed.2021.688305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265823PMC
June 2021
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