Publications by authors named "Jiaju Xu"

13 Publications

  • Page 1 of 1

Development of a four-gene prognostic model for clear cell renal cell carcinoma based on transcriptome analysis.

Genomics 2021 Apr 7;113(4):1816-1827. Epub 2021 Apr 7.

Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277 Jiefang Avenue, Wuhan 430022, China. Electronic address:

This study aimed to develop a prognostic model for clear cell renal cell carcinoma (ccRCC) based on transcriptome analysis. We screened Gene Expression Omnibus (GEO) database and the Cancer Genome Atlas (TCGA) database for gene expression data and clinical characteristics of ccRCC. After differentially expression analysis, we identified 533 key genes of the development of ccRCC. Next, a weighted gene co-expression network analysis (WGCNA) was executed to investigate the association between differentially expressed genes and clinical characteristics. Then, based on protein-protein interaction (PPI) network, least absolute shrinkage and selection operator (LASSO) regression and Cox regression, a four-gene (COL4A5, ABCB1, NR3C2 and PLG) prognostic model has been constructed in TCGA training cohort. Finally, we examined the predictive power of this model with survival analysis and receiver operating characteristic (ROC) curve both in training cohort and in validation cohorts. And we found this model was significantly correlated with infiltrating immune cells. The four-gene prognosis model could be a potential prediction tool with high accuracy and reliability for ccRCC patients.
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http://dx.doi.org/10.1016/j.ygeno.2021.04.005DOI Listing
April 2021

The Identification of Critical mA RNA Methylation Regulators as Malignant Prognosis Factors in Prostate Adenocarcinoma.

Front Genet 2020 4;11:602485. Epub 2020 Dec 4.

Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

RNA methylation accounts for over 60% of all RNA modifications, and N-methyladenosine (mA) is the most common modification on mRNA and lncRNA of human beings. It has been found that mA modification occurs in microRNA, circRNA, rRNA, and tRNA, etc. The mA modification plays an important role in regulating gene expression, and the abnormality of its regulatory mechanism refers to many human diseases, including cancers. Pitifully, as it stands there is a serious lack of knowledge of the extent to which the expression and function of mA RNA methylation can influence prostate cancer (PC). Herein, we systematically analyzed the expression levels of 35 mA RNA methylation regulators mentioned in literatures among prostate adenocarcinoma patients in the Cancer Genome Atlas (TCGA), finding that most of them expressed differently between cancer tissues and normal tissues with the significance of < 0.05. Utilizing consensus clustering, we divided PC patients into two subgroups based on the differentially expressed mA RNA methylation regulators with significantly different clinical outcomes. To appraise the discrepancy in total transcriptome between subgroups, the functional enrichment analysis was conducted for differential signaling pathways and cellular processes. Next, we selected five critical genes by the criteria that the regulators had a significant impact on prognosis of PC patients from TCGA through the last absolute shrinkage and selection operator (LASSO) Cox regression and obtained a risk score by weighted summation for prognosis prediction. The survival analysis curve and receiver operating characteristic (ROC) curve showed that this signature could excellently predict the prognosis of PC patients. The univariate and multivariate Cox regression analyses proved the independent prognostic value of the signature. In summary, our effort revealed the significance of mA RNA methylation regulators in prostate cancer and determined a mA gene expression classifier that well predicted the prognosis of prostate cancer.
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http://dx.doi.org/10.3389/fgene.2020.602485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746824PMC
December 2020

Protective effects of catalpol on mitochondria of hepatocytes in cholestatic liver injury.

Mol Med Rep 2020 Sep 13;22(3):2424-2432. Epub 2020 Jul 13.

Department of Pediatrics, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China.

Cholestasis, which is caused by the obstruction of bile flow, can lead to rapid organ injury, cell apoptosis and necrosis of hepatocytes, and may eventually develop into fibrosis and cirrhosis. Oxidative stress and mitochondrial dysfunction are the key pathogenic signs of hepatic cholestasis. Catalpol has pharmacological activities, including antioxidative and anti‑inflammatory effects, and may relieve mitochondrial damage and restore mitochondrial membrane potential. However, the potential roles and mechanisms of catalpol in cholestasis‑induced liver injury are not clear. In the present study, liver function‑related indexes were measured in the serum of mice by commercial kits. In addition, levels of serum inflammatory factors were detected by ELISA. Hematoxylin and eosin staining was performed to observe histopathological changes, and mitochondrial membrane potential was detected using JC‑1 staining. Mitochondrial adenosine triphosphate (ATP), reactive oxygen species (ROS) and malondialdehyde levels were determined using a luciferase reporter kit, flow cytometry and a thiobarbituric acid reactive substance assay kit, respectively. Western blotting was performed to detect the expression levels of apoptosis‑related proteins in liver tissues. The findings revealed that catalpol reduced liver damage caused by cholestasis, improved the mitochondrial membrane potential, and increased the ATP content and glutathione content of cholestasis model mice. Moreover, catalpol also reduced the ROS level, inhibited lipid peroxidation, and regulated oxidative stress and apoptotic protein expression. Thus, the present study preliminarily confirmed that catalpol can reduce liver injury in a mouse model of cholestasis through inhibiting oxidative stress and enhancing mitochondrial membrane potential.
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http://dx.doi.org/10.3892/mmr.2020.11337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7411478PMC
September 2020

Thermodynamics and kinetics guided probe design for uniformly sensitive and specific DNA hybridization without optimization.

Nat Commun 2019 10 14;10(1):4675. Epub 2019 Oct 14.

Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.

Sensitive and specific DNA hybridization is essential for nucleic acid chemistry. Competitive composition of probe and blocker has been the most adopted probe design for its relatively high sensitivity and specificity. However, the sensitivity and specificity were inversely correlated over the length and concentration of the blocker strand, making the optimization process cumbersome. Herein, we construct a theoretical model for competitive DNA hybridization, which disclose that both the thermodynamics and kinetics contribute to the inverse correlation. Guided by this, we invent the 4-way Strand Exchange LEd Competitive DNA Testing (SELECT) system, which breaks up the inverse correlation. Using SELECT, we identified 16 hot-pot mutations in human genome under uniform conditions, without optimization at all. The specificities were all above 140. As a demonstration of the clinical practicability, we develop probe systems that detect mutations in human genomic DNA extracted from ovarian cancer patients with a detection limit of 0.1%.
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http://dx.doi.org/10.1038/s41467-019-12593-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6791858PMC
October 2019

Combined microwave ablation and antiangiogenic therapy to increase local efficacy.

Minim Invasive Ther Allied Technol 2020 Apr 15;29(2):107-113. Epub 2019 Apr 15.

Department of Oncology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.

We aim to evaluate the efficacy, safety and survival time of microwave ablation (MWA) with adjuvant antiangiogenic therapy-endostatin in animal models. A total of 40 rabbits successfully implanted with VX2 tumors were randomly assigned to four experimental groups: Group A underwent only microwave ablation of the tumors; Group B received only antiangiogenic drugs endostatin; Group C received endostatin immediately after MWA; Group D followed up without treatment. Two months post-treatment, tumor sizes of Group A and Group C were reduced to 1.936 ± 0.373 cm and 1.592 ± 0.382 cm, respectively. However, tumors grew to 15.091 ± 1.735 cm and 47.825 ± 7.664 cm in Group B and the control group. Three months post-treatment, tumor sizes in Group A and Group C maintained as 1.395 ± 0.394 cm and 1.482 ± 0.305 cm, significantly smaller than Group B (35.277 ± 6.019 cm). All animals in the control group died, while four (40%) survived in Group B (Endo Group). The numbers of survivals in Groups A and C were seven (70%) and eight (80%), respectively. The lowest metastasis rate (2/10, 20%) was observed in Group C (combination therapy). The combination of MWA and antiangiogenic therapy triggered a significant reduction in the growth rate and metastases of tumors and may potentially improve survivals.
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http://dx.doi.org/10.1080/13645706.2019.1601632DOI Listing
April 2020

Branch-Migration Based Fluorescent Probe for Highly Sensitive Detection of Mercury.

Anal Chem 2018 10 25;90(20):11764-11769. Epub 2018 Sep 25.

Centre of Reproductive Medicine/Family Planning Research Institute, Tongji Medical College , Huazhong University of Science and Technology , Wuhan , Hubei 430030 , P.R. China.

Detection of heavy metals is of great importance for food safety and environmental analysis. Among various heavy metal ions, mercury ion is one of the most prevalent species. The methods for detection of mercury were numerous, and the T-Hg-T based assay was promising due to its simplicity and compatibility. However, traditional T-Hg-T based methods mainly relied on multiple T-Hg-T to produce enough conformational changes for further detection, which greatly restrained the limit of detection. Hence, we established a branch-migration based fluorescent probe and found that single T-Hg-T could produce strong signals. The sensing mechanism of our method in different reaction modes was explored, and the detection limits were determined to be 18.4 and 14.7 nM in first-order reaction mode and mixed reaction mode, respectively. Moreover, coupled with Endonuclease IV assisted signal amplification, the detection limit could be 1.2 nM, lower than most DNA based fluorometric assays. For practicability, the specificity of our assay toward different interfering ions was investigated and detection of Hg in deionized water and lake water was also achieved with similar recoveries compared to those of atomic fluorescence spectrometry, which demonstrated the practicability of our method in real samples. Definitely, the proposed branch migration probe would be a promising substitution for current DNA probes based on recognition of multiple T-Hg-T and we anticipate it to be widely adopted in food and environmental analysis.
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http://dx.doi.org/10.1021/acs.analchem.8b03547DOI Listing
October 2018

Effects of prophylactic oral ibuprofen on the closure rate of patent ductus arteriosus in premature infants.

Medicine (Baltimore) 2018 Sep;97(37):e12206

Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai, Shangdong Province, China.

The aim of this study was to investigate the effects of prophylactic oral ibuprofen on the closure rate of patent ductus arteriosus (PDA).This was a retrospective study and data on infants born before 36 weeks were collected. The prophylactic group was treated with ibuprofen (10, 5, and 5 mg/kg) from days 1 to 3 after birth, respectively. The conventional group was treated with the same dose of ibuprofen from days 4 to 6 once they were echocardiographically confirmed with PDA on day 3 after birth. The placebo group was treated with 5% glucose.The closure rate of PDA in the prophylactic group significantly increased on day 7 compared with the placebo group (P = .02), but showed no difference compared with the conventional group (P = .12). Serum NT-proBNP in the prophylactic and conventional groups decreased compared with the placebo group (P = .03 vs P = .07).Prophylactic oral ibuprofen can increase the closure rate of PDA in premature infants; however, it showed no significant advantages compared with conventional treatment. Serum NT-proBNP can be used to observe PDA treatment responses in premature infants.
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http://dx.doi.org/10.1097/MD.0000000000012206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155941PMC
September 2018

Endonuclease IV based competitive DNA probe assay for differentiation of low-abundance point mutations by discriminating stable single-base mismatches.

Chem Commun (Camb) 2017 Aug;53(68):9422-9425

Family Planning Research Institute/Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China.

We disclosed the unique discrimination property of Endo IV toward stable single-base mismatches located at the second nucleotide 3' to the AP site. Coupled with thermodynamic differentiation and competitive blocker strands, a highly sensitive and specific detection system was established with discrimination factors of 510-1079 for G:X mismatches and LODs of 0.003-0.005% for KRAS G12A, KRAS G12V and KRAS G12S mutations.
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http://dx.doi.org/10.1039/c7cc04816eDOI Listing
August 2017

Polypyrrole decorated BiOI nanosheets: Efficient photocatalytic activity for treating diverse contaminants and the critical role of bifunctional polypyrrole.

J Colloid Interface Sci 2017 Nov 19;505:719-727. Epub 2017 Jun 19.

Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences, Beijing 100083, China. Electronic address:

A conducting polymer polypyrrole (Ppy) was first employed to decorate BiOI for fabricating an organic-inorganic hybridized Ppy-BiOI nanocomposite photocatalyst via a facile in situ precipitation strategy at room temperature. The composite and intimate interface was confirmed by FTIR, XPS, SEM, HRTEM and TEM-mapping. In comparison with pristine BiOI, the Ppy-BiOI hybrids present significantly enhanced photocatalytic activity for degradation of Rhodamine B (RhB) under visible light (λ>420nm). Particularly, the Ppy-BiOI composite exhibits an universal photocatalytic performance for removing diverse industrial pollutants and antibiotics, including bisphenol A, 2,4-dichlorophenol, tetracycline hydrochloride and chlortetracycline hydrochloride. The enhanced photocatalytic activity of Ppy-BiOI composite is found attributable to the bifunctional role that Ppy takes. Ppy-BiOI composite has an enhanced specific surface area, which benefits adsorption and generation of more active sites. Notably, high separation and transfer of the photogenerated charge carriers was achieved on the interface between Ppy and BiOI, and the photogenerated hole transfer action of Ppy is demonstrated. Therefore, synergistic effect of adsorption-enrichment and photocatalytic degradation is realized. Our work may offer a guideline to manipulate high-performance Bi-based composite photocatalyst by coupling conducting polymers.
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http://dx.doi.org/10.1016/j.jcis.2017.06.054DOI Listing
November 2017

Ultrasound-Induced Organogel Formation Followed by Thin Film Fabrication via Simple Doctor Blading Technique for Field-Effect Transistor Applications.

ACS Appl Mater Interfaces 2016 Jul 13;8(29):18991-7. Epub 2016 Jul 13.

Department of Chemistry, South University of Science and Technology of China , Shenzhen, P. R. China.

We demonstrate doctor blading technique to fabricate high performance transistors made up of printed small molecular materials. In this regard, we synthesize a new soluble phthalocyanine, tetra-n-butyl peripheral substituted copper(II) phthalocaynine (CuBuPc), that can easily undergo gel formation upon ultrasonic irradiation, leading to the formation of three-dimensional (3D) network composed of one-dimensional (1D) nanofibers structure. Finally, taking the advantage of thixotropic nature of the CuBuPc organogel, we use the doctor blade processing technique that limits the material wastage for the fabrication of transistor devices. Due to the ultrasound induced stronger π-π interaction, the transistor fabricated by doctor blading based on CuBuPc organogel exhibits significant increase in charge carrier mobility in comparison with other solution process techniques, thus paving a way for a simple and economically viable preparation of electronic circuits.
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http://dx.doi.org/10.1021/acsami.6b04817DOI Listing
July 2016

miR-15a/16 are upreuglated in the serum of neonatal sepsis patients and inhibit the LPS-induced inflammatory pathway.

Int J Clin Exp Med 2015 15;8(4):5683-90. Epub 2015 Apr 15.

Department of Pediatrics, Yantai Yuhuangding Hospital of Qingdao University Yantai 264000, Shandong, China.

Infection in neonates, particular the neonatal sepsis continues to be a global problem with significant morbidity and mortality. The diagnosis of neonatal sepsis is complicated by nonspecific clinical symptomatology, a high-false negative rate, and a delay in obtaining blood culture results. MicroRNAs (miRNAs) have recently been used as finger prints for sepsis, and have been validated to be potential sepsis biomarker recently. In the present study, we investigated the level of several miRNAs, such as miR-15a, miR-16, miR-15b, and miR-223, which have been identified as a biomarker in adult sepsis, in neonatal sepsis patients, and then we analyzed the association of miR-15a/16 with the patient prognosis. Results demonstrated that the level of miR-15a/16 was up-regulated in neonatal sepsis patients than in normal neonatal subjects; however, no statistical difference was disclosed in the miR-15b and miR-223 level between two groups. And the ROC analysis indicated the miR-15a and miR-16 were potent fingerprints for diagnosing neonate sepsis. In order to explore the miR-15a/16 function on the lipopolysaccharide (LPS)-induced inflammatory pathway, the mice macrophage RAW264.7 cells were transiently transfected with miR-15a/16 mimics. And it was demonstrated that the miR-15a/16 transfection down-regulated the Toll-like receptor 4 (TLR4) and Interleukin-1 receptor-associated kinase 1 (IRAK-1) transcription level with a statistical difference in the LPS treated cells. And the suppression capability of miR-15a/16 on the expression of TLR-4 and IRAK-1 were evaluated by western blot. Thus, in present study, we identified miR-15a/16 as potential biomarker for the diagnosis and prognosis of neonatal sepsis, and the upregulated miR-15a/16 downregulated the LPS-induced inflammatory pathway.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4483976PMC
July 2015

Benzyl alcohol-based synthesis of oxide nanoparticles: the perspective of S(N)1 reaction mechanism.

Dalton Trans 2013 Jul 21;42(26):9777-84. Epub 2013 May 21.

Department of Physics and Materials Science, City University of Hong Kong, Hong Kong SAR, P. R. China.

In this paper, we describe a class of benzyl alcohol-based reactions for the synthesis of a series of inorganic oxide nanoparticles. Benzyl alcohol served as both the solvent and the reagent to interact with various metal chlorides for the synthesis of a series of metal oxides and compound oxides. Typical metal(IV) oxides, like TiO2, metal(III) oxides, like Fe2O3, and metal(II) oxides, like ZnO, with uniform size and special dispersibility, have been prepared through these reactions. The reaction can be illustrated from the perspective of S(N)1 reaction mechanism attributed to the easier formation of benzyl carbocation and unique reactivity of benzyloxy group with nucleophilic agents. By adjusting the experimental conditions, it was found that this type of reaction is available for the synthesis of many inorganic oxides.
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http://dx.doi.org/10.1039/c3dt50680kDOI Listing
July 2013

E1 reaction-induced synthesis of hydrophilic oxide nanoparticles in a non-hydrophilic solvent.

Nanoscale 2012 Oct;4(20):6284-8

Department of Physics and Materials Science, City University of Hong Kong, Hong Kong SAR, China.

In this paper, tert-amyl alcohol was employed to directly react with metal chlorides for the preparation of oxide nanoparticles. Some typical metal oxide or hydroxides with different morphologies, such as TiO(2) nanoparticles, TiO(2) nanorods, FeOOH nanowires, Fe(2)O(3) nanoparticles, and SnO(2) nanoparticles, can be easily fabricated through such simple chemical reactions. E1 reaction was found to play the leading role in the synthesis of metal oxides attributed to better stability of tertiary carbocations in tert-amyl alcohol and the strong interaction of metal chlorides with hydroxyl groups that results in the easy dissociation of carbon-oxygen bonds in tert-amyl alcohol. S(N)1 reaction can also occur in certain reactions due to nucleophilic substitution of chloride ions for hydroxyl groups. As-prepared metal oxides show good compatibility with an aqueous system while they were synthesized in a non-hydrophilic solvent probably attributed to the specific E1 reaction mechanism involving the generation of water, and can be directly incorporated into an aqueous soluble polymer, such as PVA, to exhibit many promising applications.
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http://dx.doi.org/10.1039/c2nr32255bDOI Listing
October 2012