Publications by authors named "Jiahui Liu"

134 Publications

Docosahexaenoic Acid in the Inhibition of Tumor Cell Growth in Preclinical Models of Ovarian Cancer.

Nutr Cancer 2021 Jul 21:1-15. Epub 2021 Jul 21.

Department of Experimental Oncology, University of Alberta, Edmonton, Alberta, Canada.

There is a strong rationale for investigating nutritional interventions with docosahexaenoic acid DHA) in cancer prevention and therapy; however, the effects of DHA on ovarian cancer (OC) have not been well studied. Here, we investigated if DHA alone and in combination with carboplatin reduces OC cell growth in vitro. In vivo, we used a high-grade serous OC patient-derived xenograft (PDX) mouse model to investigate if DHA affects OC growth and enhances the anticancer actions of carboplatin. We showed synergistic cell killing by DHA and carboplatin in DHA-resistant Kuramochi and SKOV3 OC cells, which corresponded with increased DHA incorporation into whole-cell membrane phospholipids ( < 0.05). In vivo, feeding mice a diet supplemented with 3.9% (w/w of fat) DHA resulted in a significant reduction in PDX growth with and without carboplatin ( < 0.05). This reduction in tumor growth was accompanied by an increased tumor necrotic region ( < 0.05) and improved survival. Plasma membranes in tumors and livers excised from mice fed a DHA diet had ∼ twofold increase in DHA incorporation as compared with mice fed a control diet. Our findings indicate that DHA supplementation reduces cancer cell growth and enhances the efficacy of carboplatin in preclinical models of OC through increased apoptosis and necrosis.Supplemental data for this article is available online at https://doi.org/10.1080/01635581.2021.1952453.
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http://dx.doi.org/10.1080/01635581.2021.1952453DOI Listing
July 2021

131I-radioisotope modified in PEGylation metal organic frameworks for sensitization in refractory differentiated thyroid cancer treatment.

J Biomater Appl 2021 Jul 13:8853282211029822. Epub 2021 Jul 13.

Department of Pharmacy, Harbin Medical University Cancer Hospital, Harbin, China.

Radiation therapy for cancer can lead to off-target toxicity and can be ineffective against refractory differentiated thyroid cancer. The nanoscale metal organic frameworks (NMOFs) have shown great potential in cancer diagnostic and treatment due to their advantages in the aspect of structural diversities, high intrinsic biodegradability and drug-loading capacities. Here, we provide that intratumoral injection, in mouse of refractory differentiated thyroid cancer.In this work, we used the therapeutic 131I radioisotope modified Zr-MOF ([email protected]) with aim to enable long-term relief of tumour therapy, which has successfully eliminated tumour at ralatively low radioactivity doses. Polyethylene glycol (PEG) was coated into Zr-MOF and, as a result, circulation time was significantly improved by intratumoral injection. These findings therefore suggest that nanoparticles could be used in vivo combined therapy. On injection, while it is a highly effective drug for radioisotope, Zr-MOF with attenuation ability could apply for a radio-sensitizer to enhance inner radiotherapy (RT). The local therapy, which uses only biocompatible components, might enable new strategies for local tumour treatments. These could be further combined with systemic therapeutic responses for the inhibition of refractory differentiated thyroid cancer and the prevention of tumour recurrence in patients.
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http://dx.doi.org/10.1177/08853282211029822DOI Listing
July 2021

Two-Dimensional MoO Nanosheet Composite Hydrogels with High Transmittance and Excellent Photothermal Property for Near-Infrared Responsive Actuators and Microvalves.

ACS Appl Mater Interfaces 2021 Jul 11;13(28):33404-33416. Epub 2021 Jul 11.

School of Materials Science and Engineering, Zhengzhou University, 100 Kexue Road, Zhengzhou 450001, Henan, P. R. China.

Stimulus-responsive intelligent hydrogel actuators have highly promising applications in the fields of soft robotics, smart manipulators, and flexible devices. Near-infrared (NIR) light is considered an ideal method to trigger the response behavior remotely and precisely. In order to realize the excellent optical transmittance and photothermal property of NIR-responsive hydrogels at the same time, two-dimensional nonlayered MoO nanosheets (2D-MoO) with excellent photothermal efficiency (62% under an NIR light irradiation of 808 nm), splendid chemistry stability, and low preparation cost are used as photothermal agents and incorporated into the poly(-isopropylacrylamide) (PNIPAM) hydrogel network, forming the 2D-MoO/Laponite/PNIPAM ternary nanocomposite hydrogel (TN hydrogel). It is remarkable that compared with the GO and MXene hydrogels with the same agent content (1.0 mg mL) and thickness (1 mm) whose transmittance values are only ∼5% at 600 nm, the TN hydrogel shows a similar NIR-responsive temperature, but much higher optical transmittance (∼53%). Besides, of the three hydrogels with similar transmittance, the TN hydrogel shows a much higher NIR-responsive temperature. The TN hydrogel with a low loading of 2D-MoO (1.5 mg mL) can produce a significant temperature increase of ∼30 °C after the application of 0.8 W cm NIR light irradiation for 15 s. Impressively, the TN hydrogel exhibits excellent anti-fatigue property, keeping a fast response and temperature rise behavior even after 50 times of heating-cooling cycles. The flexibly controllable and reversible deformation is realized by a well-designed bilayer structure even in harsh environments. The transparent and asymmetric bilayer hydrogel is further used as a soft manipulator to capture objects visually and accurately. The NIR light-controlled microvalve based on this composite hydrogel is also demonstrated. This work provides a novel kind of transparent hybrid NIR response hydrogel for the further development of smart, programmable, reversible hydrogel-based actuators and soft robotics.
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http://dx.doi.org/10.1021/acsami.1c04110DOI Listing
July 2021

High-resolution magnetic sensors in ferrite/piezoelectric heterostructure with giant magnetodielectric effect at zero bias field.

Rev Sci Instrum 2021 Apr;92(4):045006

Physics Department, Oakland University, Rochester, Michigan 48309, USA.

A dielectric AC magnetic sensor in layered ferrites/piezoelectric composites was fabricated and developed, whereby its high magnetodielectric (MDE) effects, the typical magnetic-sensing parameters, were systematically characterized at zero bias. Polycrystalline ferrites were synthesized by the solid-state sintering technique with a composition of NiZnTbFeO, and the desired spinel structure and soft magnetic properties were confirmed by x-ray diffraction and VSM, respectively. The field-induced charge order insulating state in piezoelectric ceramics accounts for the suppressed permittivity, which enables the possibility of a highly sensitive magnetic sensor at zero bias field. Experimental results exhibit that a small variation in H as low as 100 mOe can be clearly distinguished with a favorable nonlinearity of 2.24%. Meanwhile, the output stability of the presented sensor under 2h of constant and continuous excitation was tested within a favorable fluctuating tolerance range of 6.14-6.28 nF, and the estimated uncertainty of ∼0.063 038 nF was verified by statistical analysis. The presented ferrite/piezoelectric magnetic sensors exhibiting a high MDE response without the requirement for an external magnetic bias are of importance for use in bio-magnetic field detection due to metrics of miniaturization, high sensitivity, and favorable stabilities.
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http://dx.doi.org/10.1063/5.0035059DOI Listing
April 2021

Multiplex amplification of target genes of periodontal pathogens in continuous flow PCR microfluidic chip.

Lab Chip 2021 Jun 29. Epub 2021 Jun 29.

Oono Joint Research laboratory, Graduate school of Engineering, Osaka University, Osaka 565-0871, Japan.

Porphyromonas gingivalis (P.g), Treponema denticola (T.d), and Tannerella forsythia (T.f) are believed to be the major periodontal pathogens that cause gingivitis, which affects 50-90% of adults worldwide. Microfluidic chips based on continuous flow PCR (CF-PCR) are an ideal alternative to a traditional thermal cycler, because it can effectively reduce the time needed for temperature transformation. Herein, we explored multi-PCR of P.g, T.d and T.f using a CF-PCR microfluidic chip for the first time. Through a series of experiments, we obtained two optimal combinations of primers that are suitable for performing multi-PCR on these three periodontal pathogens, with amplicon sizes of (197 bp, 316 bp, 226 bp) and (197 bp, 316 bp, 641 bp), respectively. The results also demonstrated that by using multi-PCR, the amplification time can be reduced to as short as 3'48'' for the short-sized amplicons, while for T.f (641 bp), the minimum time required was 8'25''. This work provides an effective way to simultaneously amplify the target genes of P.g, T.d and T.f within a short time, and may promote CF-PCR as a practical tool for point-of-care testing of gingivitis.
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http://dx.doi.org/10.1039/d1lc00457cDOI Listing
June 2021

Transcriptome-Wide Analysis to Identify the Inflammatory Role of lncRNA in Experimental Ischemic Stroke.

J Inflamm Res 2021 22;14:2667-2680. Epub 2021 Jun 22.

Neurosurgery Center, Department of Cerebrovascular Surgery, The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China on Diagnosis and Treatment of Cerebrovascular Disease, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, The Neurosurgery Institute of Guangdong Province, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, People's Republic of China.

Background: Ischemic stroke is one of the leading causes of mortality and disability worldwide. Following stroke, there is secondary neuroinflammation that promotes further injury. Identifying the long non-coding RNA (lncRNA) involved in neuroinflammation after cerebral ischemic stroke will promote the discovery of potential therapeutic targets.

Methods: We identified differentially expressed genes from genome-wide RNA-seq profiles of mice with focal ischemia using Gene Ontology Term Enrichment, Kyoto Encyclopedia of Genes and Genomes, and Gene Set Enrichment analyses. Immune cell infiltration deconvolution, protein-protein interaction network construction, and co-expression network analyses were also used to screen lncRNAs. In further experiments, lncRNA knockdown animal models were developed by intraventricular injection of the antisense oligonucleotide before performing middle cerebral artery occlusion (MCAO). An enzyme-linked immunosorbent assay was performed to measure the level of cytokines. Hematoxylin-eosin staining and immunohistochemical staining were used to observe the changes in morphology.

Results: Enrichment analysis revealed that differential mRNAs induced neuroinflammation after MCAO. Immune deconvolution showed that the proportion of microglia gradually increased while monocytes decreased within 24 h. We identified six hub lncRNAs (, , , , , and ) that were highly correlated with activated-microglia mRNAs (cor > 0.8). We found that Neat1 had the highest correlation coefficient with pro-inflammatory factor mRNA levels. In vivo experiments demonstrated that had abnormally high expression after MCAO. Knockdown of could significantly alleviate brain damage by reducing the number of activated microglia and reducing their release of proinflammatory cytokines.

Conclusion: We identified inflammation-associated lncRNA as crucial, which means it is a potential target for ischemic stroke treatment.
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http://dx.doi.org/10.2147/JIR.S315281DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235937PMC
June 2021

Associations between remnant lipoprotein cholesterol and central systolic blood pressure in a Chinese community-based population: a cross-sectional study.

Lipids Health Dis 2021 Jun 26;20(1):60. Epub 2021 Jun 26.

Department of Cardiology, Peking University First Hospital, No. 8 Xishiku Street, Xicheng District, Beijing, 100034, China.

Background: The lipid profile is reportedly related to peripheral blood pressure or pulse wave velocity. However, no studies have investigated the associations between lipid parameters, especially remnant lipoprotein cholesterol (RLP-C), and central systolic blood pressure (cSBP).

Methods: This study used baseline data of a community-based cohort in Beijing, China. Participants who had been treated with anti-hypertensive or lipid-lowering agents were excluded. RLP-C is equal to total cholesterol (TC) minus the sum of low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C). An Omron HEM-9000AI device was used to measure non-invasive cSBP. The associations between blood lipid profile and non-invasive cSBP were evaluated using multivariable regression models.

Results: The 5173 included participants were 55.0 ± 8.5 years old; 35.7% (1845) of participants were men. Increased cSBP was significantly associated with increased TC, LDL-C, non-high-density lipoprotein cholesterol (non-HDL-C), triglyceride (TG), and RLP-C but with decreased HDL-C, even after adjusting for possible covariates. When simultaneously entering individual pairs of RLP-C and other blood lipid parameters into the multivariable regression model, RLP-C remained significantly associated with cSBP, even after adjusting for other lipids. Compared with participants who had RLP-C levels in the first quartile (Q1), cSBP for those with RLP-C in Q4 was increased to 4.57 (95% confidence interval [CI]: 3.08-6.06) mmHg after adjusting for LDL-C, 4.50 (95%CI: 2.98-6.02) mmHg after adjusting for TC, 3.91 (95%CI: 1.92-5.89) mmHg after adjusting for TG, 5.15 (95%CI: 3.67-6.63) mmHg after adjusting for HDL-C, and 4.10 (95%CI: 2.36-5.84) mmHg after adjusting for non-HDL-C.

Conclusions: Increased blood RLP-C level was significantly associated with higher cSBP in a Chinese population, independently of other lipids, which indicates its importance in individual cardiovascular risk assessment.
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http://dx.doi.org/10.1186/s12944-021-01490-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235613PMC
June 2021

Vehicle routing problem of contactless joint distribution service during COVID-19 pandemic.

Transp Res Interdiscip Perspect 2020 Nov 1;8:100233. Epub 2020 Oct 1.

School of Traffic and Transportation Engineering, Central South University, Changsha 410075, China.

In order to prevent the further spread of the COVID-19 virus, enclosed management of gated communities is necessary. The implementation of contactless food distribution for closed gated communities is an urgent issue. This paper proposes a contactless joint distribution service to avoid contact between couriers. Then a multi-vehicle multi-trip routing problem for contactless joint distribution service is proposed, and a mathematical programming model for this problem is established. The goal of the model is to increase residents' satisfaction with food distribution services. To solve this model, a PEABCTS algorithm is developed, which is the enhanced artificial bee colony algorithm embedded with a tabu search operator, using a progressive method to form a solution of multi-vehicle distribution routings. Finally, a variety of numerical simulations were carried out for statistical research. Compared with the two distribution services of supportive supply and on-demand supply, the proposed contactless joint distribution service can not only improve residents' satisfaction with the distribution service but also reduce the contact frequency between couriers. In addition, compared with various algorithms, it is found that the PEABCTS algorithm has better performance.
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http://dx.doi.org/10.1016/j.trip.2020.100233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528749PMC
November 2020

High-dose statin pretreatment decreases periprocedural myocardial infarction and cardiovascular events in East Asian patients undergoing percutaneous coronary intervention: A meta-analysis of fifteen randomized controlled trials.

Medicine (Baltimore) 2021 Jun;100(25):e26278

Department of Cardiology, Institute of Cardiovascular Disease, Peking University First Hospital, Beijing, China.

Background: Numerous studies have shown that high-dose statin pretreatment may reduce the risk of periprocedural myocardial infarction (PMI) and short-term major adverse cardiac events (MACE) in western people undergoing percutaneous coronary intervention (PCI). However, the effects in East Asian patients are still controversial. The objective was to evaluate the effects of short-term high-dose statin (all types) pretreatment compared with the control (low-dose or no statin) on the reduction of the rate of MACE and PMI in East Asian patients.

Methods: PubMed/Medline, EMBASE, and the Cochrane Central Register of Controlled Trials were systematically searched for randomized controlled trials (RCTs) in East Asian patients up to December 2019, in which short-term high-dose statin pretreatment was compared with control for patients undergoing PCI. The primary outcome measure was the incidence of MACE at 30 days. The secondary outcome measure was the incidence of PMI. The meta-analysis was performed with the fixed-effect model or random-effects model according to the heterogeneity. The meta-analysis was performed using RevMan 5.3 software (Cochrane Collaboration).

Results: Fifteen RCTs that enrolled 4313 East Asian patients were identified. High-dose statin pretreatment was associated with a 54% relative reduction in 30-day MACE (OR, 0.46; 95% CI, 0.31-0.67; P < .001) and a 50% relative reduction in PMI (OR, 0.50; 95% CI, 0.34-0.76; P = .001).

Conclusions: High-dose statin pretreatment can significantly reduce 30-day MACE and PMI for East Asian patients undergoing PCI.
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http://dx.doi.org/10.1097/MD.0000000000026278DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238325PMC
June 2021

Catalytically Active CoFeO Nanoflowers for Augmented Sonodynamic and Chemodynamic Combination Therapy with Elicitation of Robust Immune Response.

ACS Nano 2021 Jun 18. Epub 2021 Jun 18.

State Key Laboratory of Silkworm Genome Biology, School of Materials and Energy, Southwest University, Chongqing 400715, China.

A hypoxic and acidic tumor microenvironment (TME) plays a significant role in cancer development through complex cellular signaling networks, and it is thus challenging to completely eradicate tumors monotherapy. Here, PEGylated CoFeO nanoflowers (CFP) with multiple enzymatic activities, serving as bioreactors responsive to TME cues, were synthesized a typical solvothermal method for augmented sonodynamic therapy (SDT) and chemodynamic therapy (CDT) with elicitation of robust immune response. The CFP occupying multivalent elements (Co, Fe) exhibited strong Fenton-like and catalase-like activity. In another aspect, CFP itself is a brand-new sonosensitizer for high-performance SDT based on ultrasound-triggered electron (e)/hole (h) pair separation from the energy band with promptness and high efficiency. With efficient enrichment in tumorous tissue as revealed by magnetic resonance imaging, CPF could generate OH for CDT relying on Fenton-like reactions. Moreover, catalase-mimicking CFP could react with endogenous HO to generate molecular oxygen, and high O level may promote the production of O for SDT. What's more, the reactive oxygen species obtained from combined SDT/CDT could efficiently trigger immunogenic cell death through a synergistic therapy based on the elicitation of antitumor immunity with the aid of an immune checkpoint blockade for the sake of suppressing primary and distant tumors as well as lung metastasis. Taken together, this paradigm delivers useful insights for developing in-coming nanocomposites based on cobalt ferrite for cancer theranostics.
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http://dx.doi.org/10.1021/acsnano.1c03128DOI Listing
June 2021

Niujiaodihuang Detoxify Decoction inhibits ferroptosis by enhancing glutathione synthesis in acute liver failure models.

J Ethnopharmacol 2021 Jun 12;279:114305. Epub 2021 Jun 12.

School of Pharmacy, Guangdong Medical University, Dongguan, 524023, China. Electronic address:

Ethnopharmacological Relevance: Niujiaodihuang Detoxify Decoction (NDD) is an integrated traditional Chinese medicine prescription that has been used as a therapeutic agent for the treatment of acute liver failure (ALF). However, the mechanisms underlying its action remain unclear.

Aim Of The Study: To determine the protective effect of NDD on D-galactosamine/lipopolysaccharide (D-GalN/LPS)-induced ALF and explore the underlying mechanisms.

Materials And Methods: We characterized the NDD fingerprint by HPLC and established D-GalN/LPS-induced ALF models in Sprague-Dawley rats and LO2 cells. Next, we measured the protective and antiferroptotic effects of NDD in vivo and in vitro. To further investigate the molecular mechanisms underlying the effects of NDD, we performed metabolomic analysis of the liver tissue using LC-MS/MS.

Results: Results of serum biochemical analysis, liver histopathology, and cell viability showed that NDD effectively relieved the liver injury. It reduced the accumulation of labile iron and alleviated lipid peroxidation by enhancing GPX4 activity. The mitochondrial morphology indicated that NDD exerted its hepatoprotective effect through an antiferroptotic activity. Metabolomic analysis showed that NDD treatment increased the levels of cysteine, decreased those of glutamate, and ameliorated the D-GalN/LPS-induced reduction in the levels of glutathione (GSH). The results for intracellular levels of reduced (GSH) and oxidized (GSSG) glutathione were consistent with those of metabolomic analysis.

Conclusion: Our findings indicate that NDD exerts hepatoprotective activity by evoking the reprogramming of GSH metabolism, and thereby, inhibiting ferroptosis.
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http://dx.doi.org/10.1016/j.jep.2021.114305DOI Listing
June 2021

Programmable Color in a Free-Standing Photonic Microgel Film with Ultra-Fast Response.

ACS Appl Mater Interfaces 2021 Jun 20;13(21):25563-25570. Epub 2021 May 20.

Center for Bioengineering and Biotechnology, College of Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, China.

In this work, a free-standing microgel film with programmable and angle-independent structural color is prepared via a simple but effective method. Dried poly(styrene--isopropylacrylamide-acrylic acid) (pStNIPAAmAA) microgels were stabilized by inter-microgel crosslinking, and thus, only microgels were used to build the optical hydrogel. The free-standing microgel film displayed tunable structural color by the swelling/deswelling of the microgels under external stimuli, such as temperature, pH, ionic strength, and organic solvent. Moreover, the structural color of the film is angle-independent for the disordered microgel arrays. It is worth noting that programmable color stripes which have the panther chameleon's ability to change skin color are successfully fabricated by patterning microgels with different thermoresponsivities. More importantly, the microgel film has an ultrafast response to temperature (1.41 s from 20 to 40 °C) and pH (2.24 s from pH 8.3 to pH 2.0), much faster than that of most optical materials reported in previous studies.
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http://dx.doi.org/10.1021/acsami.1c07099DOI Listing
June 2021

Evaluating the efficacy and safety of immune checkpoint inhibitors by detecting the exposure-response: An inductive review.

Int Immunopharmacol 2021 Apr 30;97:107703. Epub 2021 Apr 30.

Department of Pharmacy, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China. Electronic address:

Immune checkpoint inhibitors (ICIs) have been demonstrated an effective treatment in multiple tumor type, which restore the immune response to against cancer cell. Currently, approved ICIs include anti-cytotoxic T-lymphocyte antigen-4 (CTLA-4); anti-programmed cell death 1 (PD-1) and anti-programmed cell death ligand 1 (PD-L1) monoclonal antibodies (mAbs). In most these drugs, unique pharmacokinetic (PK) and pharmacodynamics (PD) have shown significant influence on clinical outcomes, which occurred by target-mediated drug concentration and time-varying drug clearance. An exposure-response (E-R) relationship has been used to describe the safety and efficacy of ICIs, and shown a plateaued E-R and time dependent changes in exposure. Using an enzyme linked immunosorbent assay (ELISA) or LC-MS/MS method to measure the peak concentration, trough concentration or area under the curve (AUC) of ICIs to assess the drug exposure. There are lots of covariates that have an influence on exposure, such as sex, clearance, body weight and tumor burden. In this review, we pooled data from studies of concentration or other pharmacokinetics parameter of mAbs to assess E-R in efficacy and safety.
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http://dx.doi.org/10.1016/j.intimp.2021.107703DOI Listing
April 2021

Corrigendum: Combination of Tanshinone IIA and Cisplatin Inhibits Esophageal Cancer by Downregulating NF-κB/COX-2/VEGF Pathway.

Front Oncol 2021 6;11:670798. Epub 2021 Apr 6.

Department of Traditional Chinese Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

[This corrects the article DOI: 10.3389/fonc.2020.01756/full.].
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http://dx.doi.org/10.3389/fonc.2021.670798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8057108PMC
April 2021

Bone marrow stem cells secretome accelerates simulated birth trauma-induced stress urinary incontinence recovery in rats.

Aging (Albany NY) 2021 03 31;13(7):10517-10534. Epub 2021 Mar 31.

Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.

Stress urinary incontinence (SUI) is defined as involuntary urine leakage during physical activities that increase the intra-abdominal pressure on the bladder. We studied bone marrow stem cell (BMSC) secretome-induced activation of anterior vaginal wall (AVW) fibroblasts and its ability to accelerate SUI recovery following vaginal distention (VD) in a rat model of birth trauma using BMSC-conditioned medium (BMSC-CM) and concentrated conditioned medium (CCM). BMSC-CM enhanced the proliferation, migration, and collagen synthesizing abilities of fibroblasts. Differentially expressed genes in BMSC-CM-induced fibroblasts were mainly enriched for cell adhesion, extracellular fibril organization and angiogenesis. Treatment with the JAK2 inhibitor AG490 reversed BMSC-CM-induced activation of the JAK2/STAT4 pathway. Periurethral injection with BMSC-CCM markedly enhanced the abdominal leak point pressure (LPP) in rats after VD. Histological analysis revealed increased numbers of fibroblasts, improved collagen fibers arrangement and elevated collagens content in the AVW of rats receiving BMSC-CCM. These findings suggest the BMSC secretome activates AVW fibroblasts and contributes to the functional and anatomic recovery of simulated birth trauma-induced SUI in rats.
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http://dx.doi.org/10.18632/aging.202812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064190PMC
March 2021

Embryonic protein NODAL regulates the breast tumor microenvironment by reprogramming cancer-derived secretomes.

Neoplasia 2021 04 27;23(4):375-390. Epub 2021 Mar 27.

Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada; Department of Oncology, University of Alberta, Edmonton, AB, Canada. Electronic address:

The tumor microenvironment (TME) is an important mediator of breast cancer progression. Cancer-associated fibroblasts constitute a major component of the TME and may originate from tissue-associated fibroblasts or infiltrating mesenchymal stromal cells (MSCs). The mechanisms by which cancer cells activate fibroblasts and recruit MSCs to the TME are largely unknown, but likely include deposition of a pro-tumorigenic secretome. The secreted embryonic protein NODAL is clinically associated with breast cancer stage and promotes tumor growth, metastasis, and vascularization. Herein, we show that NODAL expression correlates with the presence of activated fibroblasts in human triple-negative breast cancers and that it directly induces Cancer-associated fibroblasts phenotypes. We further show that NODAL reprograms cancer cell secretomes by simultaneously altering levels of chemokines (e.g., CXCL1), cytokines (e.g., IL-6) and growth factors (e.g., PDGFRA), leading to alterations in MSC chemotaxis. We therefore demonstrate a hitherto unappreciated mechanism underlying the dynamic regulation of the TME.
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http://dx.doi.org/10.1016/j.neo.2021.02.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041663PMC
April 2021

New slow release mixture of (E)-β-farnesene with methyl salicylate to enhance aphid biocontrol efficacy in wheat ecosystem.

Pest Manag Sci 2021 Jul 25;77(7):3341-3348. Epub 2021 Apr 25.

College of Plant Protection, Shandong Agricultural University, Taian, China.

Semiochemical use is a promising way to reduce damage from pests by improving natural control in agro-ecosystems. The aphid alarm pheromone (E)-β-farnesene (EβF) and herbivore-induced methyl salicylate (MeSA) are two volatile cues to induce changes in aphid behavior with functional significance. Because of limitations related to the volatility and oxidization of EβF and MeSA under natural conditions, slow-release and antioxidant techniques should be developed and optimized before application. Here, a slow-release alginate bead of EβF mixed with MeSA was first designed and manufactured. We hypothesized that a mixture of these two semiochemicals could be effective in controlling Sitobion miscanthi in wheat crops. Both MeSA and EβF in alginate beads were released stably and continuously for at least 15 days in the laboratory, whereas EβF in paraffin oil and pure MeSA were released for only 2 and 7 days, respectively. In 2018 field experiments, EβF and MeSA alone or in association significantly decreased the abundance of alate and apterous aphids. An increased abundance of mummified aphids enhanced by higher parasitism rates was observed when using EβF and MeSA in association, with a significant reduction of apterous abundance, more so than EβF or MeSA alone. In 2019, plots treated with a mixture of EβF and MeSA showed significantly decreased abundance of alate and apterous aphids with higher parasitism rates compared with the control. The new slow-release alginate bead containing a mixture of EβF with MeSA could be the most efficient formulation to control S. miscanthi population by attracting parasitoids in the wheat agro-ecosystem. © 2021 Society of Chemical Industry. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/ps.6378DOI Listing
July 2021

Resolvin D1 ameliorates Inflammation-Mediated Blood-Brain Barrier Disruption After Subarachnoid Hemorrhage in rats by Modulating A20 and NLRP3 Inflammasome.

Front Pharmacol 2020 3;11:610734. Epub 2021 Feb 3.

Neurosurgery Center, Department of Cerebrovascular Surgery, The National Key Clinical Specialty, Engineering Technology Research Center of Education Ministry of China on Diagnosis and Treatment of Cerebrovascular Disease, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, The Neurosurgery Institute of Guangdong Province, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Inflammation is typically related to dysfunction of the blood-brain barrier (BBB) that leads to early brain injury (EBI) after subarachnoid hemorrhage (SAH). Resolvin D1 (RVD1), a lipid mediator derived from docosahexaenoic acid, possesses anti-inflammatory and neuroprotective properties. This study investigated the effects and mechanisms of RVD1 in SAH. A Sprague-Dawley rat model of SAH was established through endovascular perforation. RVD1was injected through the femoral vein at 1 and 12 h after SAH induction. To further explore the potential neuroprotective mechanism, a formyl peptide receptor two antagonist (WRW4) was intracerebroventricularly administered 1 h after SAH induction. The expression of endogenous RVD1 was decreased whereas A20 and NLRP3 levels were increased after SAH. An exogenous RVD1 administration increased RVD1 concentration in brain tissue, and improved neurological function, neuroinflammation, BBB disruption, and brain edema. RVD1 treatment upregulated the expression of A20, occludin, claudin-5, and zona occludens-1, as well as downregulated nuclear factor-κBp65, NLRP3, matrix metallopeptidase 9, and intercellular cell adhesion molecule-1 expression. Furthermore, RVD1 inhibited microglial activation and neutrophil infiltration and promoted neutrophil apoptosis. However, the neuroprotective effects of RVD1 were abolished by WRW4. In summary, our findings reveal that RVD1 provides beneficial effects against inflammation-triggered BBB dysfunction after SAH by modulating A20 and NLRP3 inflammasome.
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http://dx.doi.org/10.3389/fphar.2020.610734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957930PMC
February 2021

Weak Light-Stimulated Synaptic Hybrid Phototransistors Based on Islandlike Perovskite Films Prepared by Spin Coating.

ACS Appl Mater Interfaces 2021 Mar 10;13(11):13362-13371. Epub 2021 Mar 10.

National & Local United Engineering Laboratory of Flat Panel Display Technology, Fuzhou University, Fuzhou 350108, P. R. China.

An artificial synaptic device that can provide color discrimination, image storage, and image recognition is highly required to mimic the human vision for biological robots. All-inorganic halide perovskites have attracted extensive attention for the reason of their high stability and favorable photoelectric properties. In this study, a light-stimulated synaptic phototransistor based on a CsPbBr/organic semiconductor hybrid film is reported. The fabricated CsPbBr film exhibits an island structure, which reduces the hysteresis effectively and at the same time achieves a high specific detectivity of up to 2 × 10 Jones. The decay of the photocurrent can be delayed by changing the gate bias, which is essential for achieving high-performance light-stimulated synaptic devices. Due to the outstanding detectivity of the device, the obvious synaptic functions can be observed when triggered by a light signal with a power of 1.6 nW that is much weaker than previous most perovskite-based hybrid synaptic phototransistors under a low operating voltage of -1 V. The electrical power consumption of the device could be as low as 0.076 pJ when the power of light spike was 7.36 nW. Taking into account this characterization, with changing of light intensity or wavelength, the contrast of the image was enlarged, which can further promote the image recognition accuracy. More significantly, this CsPbBr/TIPS hybrid film can be fabricated by facile and low-cost solution processes. This study indicates the great potential of solution-processed perovskite-based light-stimulated synapses for future artificial visual systems.
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http://dx.doi.org/10.1021/acsami.0c22604DOI Listing
March 2021

Acetabular development and fate of inverted limbus in rabbits: Experimental observation from an animal model.

J Orthop Res 2021 Feb 12. Epub 2021 Feb 12.

Department of Pediatric Orthopedics, Shengjing Hospital of China Medical University, Shenyang, China.

Using an animal model, we aimed to investigate the effects of an inverted limbus on acetabular development following closed reduction of developmental dysplasia of the hip (DDH). We interpositioned the menisci of 5-week-old rabbits (n = 40) into the hip joints to simulate limbus inversion following closed reduction for DDH. The acetabular index (AI) on anteroposterior pelvic radiographs and magnetic resonance imaging were used to evaluate acetabular development. Animals were euthanized at 4 and 8 weeks after surgery. Histological sections of the acetabular cartilage were stained and scored in accordance with the modified Mankin system. Scanning electron microscopy and transmission electron microscopy were used to examine the ultrastructure of the acetabular cartilage. Terminal deoxynucleotidyl transferase dUTP nick end-labeling staining was used to evaluate chondrocyte apoptosis. Immunohistochemistry and Western blot analyses were used to examine the expression of type X collagen (Col-X) and matrix metalloproteinase 13 (MMP-13) in the acetabular cartilage. AI values increased over a period and were higher in the experimental group than in the sham group. In the experimental group, the acetabular surface had become rough and had split in some cases. Chondrocytes within the acetabular cartilage had become hypertrophic, gradually forming clusters, and taking on an apoptotic appearance. Col-X and MMP-13 expression also increased with time. Our findings suggest that residual limbus inversion following closed reduction for DDH can cause progressive dysplasia of the acetabulum, apoptosis of acetabular chondrocytes, accelerated cartilage degeneration, and even early-stage osteoarthritis.
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http://dx.doi.org/10.1002/jor.25005DOI Listing
February 2021

The relation between plasma miR-126 levels and cerebral collateral circulation in patients with intracranial arterial stenosis.

Neurol Neurochir Pol 2021 9;55(3):281-288. Epub 2021 Feb 9.

Department of Neurology, Baotou Central Hospital, Inner Mongolia, China.

Objective: This study aimed to investigate the correlation between the circulating miR-126 regulation pathway and the cerebral collateral circulation (CCC), and to test whether miR-126 could serve as a potential biomarker for CCC formation in patients with intracranial arterial stenosis or occlusion.

Material And Methods: This single-centre cross-sectional study enrolled patients who underwent cerebral angiography with severe stenosis (≥70%) or occlusion in at least one major intracranial artery. Collateral degree was graded according to the ASITN/SIR classification. The patients were divided into a good CCC group (grade 3-4) or a poor CCC group (grade 0-2). We investigated the plasma levels of miR-126, VEGF, Spred-1 and PIK3R2 by using qRT-PCR, ELISA and Western blot methods, respectively. In addition, we assessed the correlations of plasma miR-126 with VEGF, Spred-1, PIK3R2 and ASITN/SIR grade using the Spearman correlation test and investigated its predictive power for CCC status by using the receiver operating characteristic curve.

Results: A total of 68 patients were enrolled (44 with good CCC and 24 with poor CCC). Data showed that plasma miR-126 and VEGF were significantly higher in the good CCC group than in the poor CCC group. Plasma Spred-1 and PIK3R2 level were lower in the good CCC group than in the poor CCC group. In addition, miR-126 and VEGF were positively correlated with ASITN/SIR (miR-126: R = 0.595, P < 0.01; VEGF: R = 0.595, P < 0.01), whereas Spred-1 and PIK3R2 were negatively correlated with ASITN/SIR (Spred-1: R = -0.817, P < 0.01; PIK3R2: R = -0.513, P=0.01). However, the area under the curve of miR-126 level for CCC status was only 0.328 (95% CI: 0.158-0.498; p = 0.067).

Conclusions: Plasma miR-126 level may be related to better CCC formation, one of the mechanisms that may be explained by upregulation of VEGF and reduction of Spred-1 and PIK3R2 protein expression. However, miR-126 might not be an independent predictor for CCC, given its low predictive value.
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http://dx.doi.org/10.5603/PJNNS.a2021.0019DOI Listing
July 2021

The complete chloroplast genome of and phylogenic analysis of Lamiales.

Mitochondrial DNA B Resour 2020 Oct 12;5(3):3561-3562. Epub 2020 Oct 12.

School of Life Sciences, Henan University, Kaifeng, China.

is a national rare and Endangered tree in the Oleaceae family, that has high commercial value owing to its slow growth, interlaced roots, intricate tree shape, and easy to shape. Here, we determined the complete chloroplast (cp) genome sequence for using genome skimming sequencing. The cp genome was 155,698 bp and consisted of a large single copy (LSC) region (86,498 bp), a small single copy (SSC) region (17,803 bp) and two inverted repeats (IRs) (25,694 bp). It encodes 131 genes, including 88 protein-coding genes, 8 rRNAs and 35 tRNAs. Phylogenetic analysis indicates that was relatively closely related to compared to other species in the Oleaceae family.
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http://dx.doi.org/10.1080/23802359.2020.1829128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782264PMC
October 2020

Rupture Risk Assessment for Cerebral Aneurysm Using Interpretable Machine Learning on Multidimensional Data.

Front Neurol 2020 23;11:570181. Epub 2020 Dec 23.

National Key Clinical Specialty/Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Neurosurgery Institute, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

Assessment of cerebral aneurysm rupture risk is an important task, but it remains challenging. Recent works applying machine learning to rupture risk evaluation presented positive results. Yet they were based on limited aspects of data, and lack of interpretability may limit their use in clinical setting. We aimed to develop interpretable machine learning models on multidimensional data for aneurysm rupture risk assessment. Three hundred seventy-four aneurysms were included in the study. Demographic, medical history, lifestyle behaviors, lipid profile, and morphologies were collected for each patient. Prediction models were derived using machine learning methods (support vector machine, artificial neural network, and XGBoost) and conventional logistic regression. The derived models were compared with the PHASES score method. The Shapley Additive Explanations (SHAP) analysis was applied to improve the interpretability of the best machine learning model and reveal the reasoning behind the predictions made by the model. The best machine learning model (XGBoost) achieved an area under the receiver operating characteristic curve of 0.882 [95% confidence interval (CI) = 0.838-0.927], significantly better than the logistic regression model (0.779; 95% CI = 0.729-0.829; = 0.002) and the PHASES score method (0.758; 95% CI = 0.713-0.800; = 0.001). Location, size ratio, and triglyceride level were the three most important features in predicting rupture. Two typical cases were analyzed to demonstrate the interpretability of the model. This study demonstrated the potential of using machine learning for aneurysm rupture risk assessment. Machine learning models performed better than conventional statistical model and the PHASES score method. The SHAP analysis can improve the interpretability of machine learning models and facilitate their use in a clinical setting.
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http://dx.doi.org/10.3389/fneur.2020.570181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785850PMC
December 2020

Molecular characterization and expression dynamics of three key genes in the PI3K-AKT pathway reveal its involvement in the immunotoxicological responses of the giant river prawn Macrobrachium rosenbergii to acute ammonia and nitrite stress.

Ecotoxicol Environ Saf 2021 Jan 11;208:111767. Epub 2020 Dec 11.

College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong Province, PR China; Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Guangdong Ocean University, Zhanjiang, Guangdong Province, PR China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Province, PR China. Electronic address:

Ammonia nitrogen and nitrite are two common forms of environmental toxicants for aquatic organisms including crustaceans. The PI3K-AKT pathway is an important intracellular signaling pathway related to cellular stress response, but involvement of this pathway in the immunotoxicological response of decapod crustaceans to aquatic toxicants such as ammonia nitrogen and nitrite still remains enigmatic. In this study, based on transcriptome mining and molecular cloning techniques, three key genes (named as MrPI3K, MrAKT and MrFoxO) in the PI3K-AKT signaling pathway were identified from the giant river prawn Macrobrachium rosenbergii. Sequence homology and phylogenetic analysis revealed that all the three genes harbored signature sequences of corresponding protein families, and shared high levels of similarities with their respective homologs from other species. MrPI3K, MrAKT and MrFoxO all displayed ubiquitous tissue distribution profiles, but their expression levels varied to a great extend among different tissues and between sexes. Following exposure to nitrite (20 mg/L nitrite-N) or ammonia (25 mg/L total ammonia-N) stresses for 24 h and 48 h, the three genes all responded by altering their expression levels at different time points, but they didn't show uniform expression patterns following these stresses, indicating the diversified roles of these genes in different tissues and the complexity of this signaling pathway. Remarkably, MrPI3K and MrAKT were induced only in the hemocytes and intestine, respectively, indicating their specific roles in these organs. Our study demonstrated the potential utility of these genes as biomarkers of acute ammonia or nitrite toxicity in prawns, and also provided evidence that the PI3K-AKT pathway is involved in the immunotoxicological responses to nitrite and ammonia stress in M. rosenbergii.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111767DOI Listing
January 2021

Solid-State Fermentation of to Optimize Extraction Process of Isoliquiritigenin from .

Evid Based Complement Alternat Med 2020 28;2020:8927858. Epub 2020 Nov 28.

Center for Life Science and Enviromental Science, Harbin University of Commerce, Harbin 150076, China.

We successfully extracted isoliquiritigenin from via fermentation with and ultrasonic-assisted extraction. In brief, we used fermentation to culture powder, and we optimized some key parameters such as reaction conditions of pH, inoculation concentration of , fermentation time, and solid-liquid ratio. Based on a single-factor experiment, we utilized the response surface methodology (RSM) approach to optimize this extraction procedure. Using the RSM approach, optimized conditions of pH = 3.694, the solid-liquid ratio = 1 : 2.155, and the inoculation concentration of  = 1466745 were selected. Optimized conditions resulted in an extraction efficiency of 1.525 mg/g. These results showed that the extraction of isoliquiritigenin was most affected by pH and then the time of fermentation and the solid-liquid ratio. Overall, the developed extraction technique yielded 5 times the amount of isoliquiritigenin when compared to traditional methods.
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http://dx.doi.org/10.1155/2020/8927858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7737459PMC
November 2020

Predictors of favorable outcomes for vertebrobasilar artery occlusion after endovascular therapy within 24 hours of symptom onset.

Clin Neurol Neurosurg 2021 02 8;201:106422. Epub 2020 Dec 8.

Department of Neurology, Baotou Central Hospital, Baotou, Inner Mongolia, China. Electronic address:

Objective: The aim of the present study was to describe our results of endovascular therapy (EVT) for vertebrobasilar artery occlusion (VBAO) within 24 h of symptom onset, and to evaluate prognostic factors associated with favorable outcomes.

Methods: The present study enrolled patients who underwent EVT for acute ischemic stroke (AIS) caused by VBAO. Inclusion criteria for EVT to treat VBAO were as follows: (1) computed tomography angiography (CTA) or magnetic resonance angiography (MRA) confirmed acute VBAO; (2) baseline National Institutes of Health Stroke Scale (NIHSS) score ≥2; (3) premorbid modified Rankin scale (mRS) score ≤2; (4) onset or last known time to puncture within 24 h; and (5) posterior-circulation Acute Stroke Prognosis Early CT score (pc-ASPECTS) ≥6. Favorable outcomes were defined as mRS scores of 0-3 at three months following EVT. The associations among baseline parameters, procedural parameters, and favorable outcomes were assessed.

Results: A total of 67 patients were recruited in this study, of which 40 patients (59.7 %) had favorable outcomes. Of the 50 patients with a late-window (6-24 h), 29 patients (58 %) had favorable outcomes. Univariate analysis revealed significant associations of the following parameters with favorable outcomes in the enrolled patients: age, sex, smoking status, baseline NIHSS score, baseline Glasgow coma scale (GCS) score, Pons-midbrain index (PMI), and intracranial atherosclerosis (ICAS). Multivariate logistic regression indicated that only age (OR 0.914, 95 % CI: 0.849 to 0.984; p = 0.017), baseline GCS score (OR 1.234, 95 % CI: 1.061-1.435; p = 0.006), and PMI (OR 0.448, 95 % CI: 0.252 to 0.798; p = 0.006) were independently associated with favorable outcomes at three months following EVT. After adjustments for confounding factors in patients with a late-window, only age (OR 0.879, 95 % CI: 0.799 to 0.967; p = 0.008) was associated with favorable outcomes.

Conclusions: Younger age, lower PMI, and higher GCS scores in patients with VBAO-induced AIS were associated with more favorable outcomes. In late-window (6-24 h) patients, younger age associated to favorable outcomes, and lower NIHSS scores and lower PMI each also had a tendency to associated with favorable outcomes.
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http://dx.doi.org/10.1016/j.clineuro.2020.106422DOI Listing
February 2021

Assessment of atmospheric pollutant emissions with maritime energy strategies using bayesian simulations and time series forecasting.

Environ Pollut 2021 Feb 24;270:116068. Epub 2020 Nov 24.

School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore. Electronic address:

With increasingly stringent regulations on emission criteria and environment pollution concerns, marine fuel oils (particularly heavy fuel oils) that are commonly used today for powering ships will no longer be allowed in the future. Various maritime energy strategies are now needed for the long-term upgrade that might span decades, and quantitative predictions are necessary to assess the outcomes of their implementation for decision support purpose. To address the technical need, a novel approach is developed in this study that can incorporate the strategic implementation of fuel choices and quantify their adequacy in meeting future environmental pollution legislations for ship emissions. The core algorithm in this approach is based on probabilistic simulations with a large sample size of ship movement in the designated port area, derived using a Bayesian ship traffic generator from existing real activity data. Its usefulness with scenario modelling is demonstrated with application examples at five major ports, namely the Ports of Shanghai, Singapore, Tokyo, Long Beach, and Hamburg, for assessment at Years 2020, 2030, and 2050 with three economic scenarios. The included fuel choices in the application examples are comprehensive, including heavy fuel oils, distillates, low sulphur fuel oils, ultra-low sulphur fuel oils, liquefied natural gas, hydrogen, biofuel, methanol, and electricity (battery). Various features are fine-tuned to reflect micro-level changes on the fuel choices, terminal location, and/or ship technology. Future atmospheric pollutant emissions with various maritime energy strategies implemented at these ports are then discussed comprehensively in details to demonstrate the usefulness of the approach.
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http://dx.doi.org/10.1016/j.envpol.2020.116068DOI Listing
February 2021

Optimal vehicle size and driving condition for extended-range electric vehicles in China: A life cycle perspective.

PLoS One 2020 20;15(11):e0241967. Epub 2020 Nov 20.

School of Automobile, Chang'an University, Xi'an, China.

Many researchers use life cycle assessment methodology to investigate the energy and environmental impacts of energy-saving and new energy vehicles. However, in the context of China, the life cycle energy-saving and emission-reduction effects of extended-range electric vehicles (EREVs), and the optimal applicable vehicle size and driving conditions for EREVs have been rarely studied. In this study, based on the life cycle assessment theory, the resource consumption, energy exhaustion, and environmental impact of EREVs were comprehensively analyzed. In addition, a differential evaluation model of ecological benefits was established for comparing EREVs with other vehicles with different power sources. Finally, scenario analysis was performed in terms of different vehicle sizes and driving conditions. The results have shown that EREV has great advantages in reducing mineral resource consumption and fossil energy consumption. The consumption of mineral resources of EREV is 14.68% lower than that of HEV, and the consumption of fossil energy is 34.72% lower than that of ICEV. In terms of environmental impact, EREV lies in the middle position. The scenario analysis has revealed that, for EREV in China, the optimal vehicle size is the passenger car and the optimal driving condition is the suburban condition. This work helps to understand the environmental performance of EREVs in China and may provide a decision-making reference for the government.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0241967PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7679024PMC
December 2020

Sorafenib and CuB exert synergistic antitumor effects against hepatocellular carcinoma cells via inhibition of STAT3 phosphorylation.

FEBS Open Bio 2021 01 27;11(1):133-145. Epub 2020 Nov 27.

Cancer Center, The General Hospital of Western Theater Command, Chengdu, China.

Sorafenib, the first-line agent for treatment of advanced hepatocellular carcinoma (HCC), improves median overall survival by approximately 3 months. In the present study, we investigated whether sorafenib combined with cucurbitacin B (CuB), a natural tetracyclic triterpenoid isolated from Cucurbitaceae, exerts enhanced antitumor effects against HCC. Cell viability and colony formation ability were detected by cell-counting kit-8 and colony formation assays. Cell cycle and apoptosis were analyzed by flow cytometry. Protein expression was detected by western blotting. HepG2 xenografts in nude mice were used to evaluate in vivo antitumor effects. We report that sorafenib and CuB exhibited synergistic effects on cellular proliferation inhibition and cell apoptosis induction, but not on cell cycle arrest. Furthermore, combination treatment enhanced levels of cleaved caspase 3 and cleaved caspase 9, but suppressed phosphorylation of STAT3. Epidermal growth factor, a potent stimulator of signal transducer and activator of transcription-3 (STAT3), promoted cell viability and colony formation ability, whereas combination treatment exerted inhibitory effects on epidermal growth factor-induced STAT3 phosphorylation. Finally, HepG2 xenograft mice cotreated with sorafenib and CuB exhibited reduced tumor progression without notable weight loss. In conclusion, sorafenib and CuB exert synergistic antitumor effects through a pathway that may involve STAT3 phosphorylation, and this may represent a promising therapeutic approach for treatment of HCC.
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http://dx.doi.org/10.1002/2211-5463.13035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780105PMC
January 2021

T2 mapping of the acetabular cartilage in infants and children with developmental dysplasia of the hip.

Acta Radiol 2020 Oct 27:284185120966684. Epub 2020 Oct 27.

Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, Liaoning Province, PR China.

Background: T2 mapping is useful for evaluating the cartilage matrix.

Purpose: To determine the variations in the acetabular cartilage T2 relaxation values between healthy individuals and those with developmental dysplasia of the hip (DDH).

Material And Methods: Thirty-three patients with unilateral DDH underwent 3-T magnetic resonance imaging (MRI) between January 2018 and February 2019. Fifteen volunteers (30 hips) were enrolled as controls. T2 values were measured with the T2 mapping sequence in all layers and were equally divided into three layers (deep, middle, and superficial) with equal thickness. We calculated the mean T2 relaxation values for the full thickness, deep, middle, and superficial layers and compared the values between the different groups. In addition, the inter- and intra-observer agreements were calculated.

Results: The T2 relaxation values in the DDH arm were significantly lower in the middle, superficial, and full thickness layers compared with those of the volunteers and contralateral hips. The T2 relaxation values of the deep layers showed no significant difference between the different groups. The acetabular cartilage T2 relaxation values increased from the deep layer to the superficial layer in the control and contralateral groups. Both inter- and intra-observer agreements were good.

Conclusion: MRI T2 mapping may help to diagnose developmental disorders of the acetabular cartilage matrix in infants and children with DDH. Abnormal acetabular cartilage T2 relaxation values may be due to the extraordinary stress load of the femoral head.
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http://dx.doi.org/10.1177/0284185120966684DOI Listing
October 2020