Publications by authors named "Jiahuan Wang"

10 Publications

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Galectin-3/adiponectin as a new biological indicator for assessing the risk of type 2 diabetes: a cross-sectional study in a community population.

Aging (Albany NY) 2021 06 7;13(11):15433-15443. Epub 2021 Jun 7.

Department of Endocrinology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, People's Republic of China.

Objective: This study aimed to explore the association between the risk of newly diagnosed type 2 diabetes and galectin-3 and adiponectin and to investigate whether their joint action shows a favorable diabetes assessment performance.

Methods: We conducted a community-based study in 135 newly diagnosed patients with type 2 diabetes and 270 age- and sex-matched nondiabetic patients. Odds ratios and 95% confidence intervals were determined using logistic regression analysis. Receiver operating characteristic curve, decision curve analysis and calibration plot were used to explore their efficacy and clinical utility for models.

Results: High quartiles of galectin-3/adiponectin (quartile 4 vs 1: OR 2.43 [95% CIs: 1.21-5.00]) showed the strongest correlation with an increased risk of type 2 diabetes in the total population, which was consistent in the older population (age≥50 years old) in adjustment models. The combination + lipids + galectin-3/adiponectin model (AUC = 0.72 [95% CIs: 0.66-0.77]) displayed better diabetes assessment performance than the other two models.

Conclusions: High galectin-3 and low adiponectin levels were associated with the high risk of diabetes, and their joint action was a superior promising factor for evaluating diabetes risk. The diabetes discriminative strength of galectin-3/adiponectin was better in the older population than the younger.
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http://dx.doi.org/10.18632/aging.203101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221326PMC
June 2021

Smartphone as a simple device for visual and on-site detection of fluoride in groundwater.

J Hazard Mater 2021 06 19;411:125182. Epub 2021 Jan 19.

Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, PR China.

Developing a portable device for visual and on-site detection of fluoride in groundwater is highly anticipated. In this paper, 2-(tert-butyl-diphenylsilanyloxy)-5-nitro-1H-benzoimidazole (1) has been rationally designed via a silanization reaction for self-calibration detection of fluoride, and the detection limit was calculated as 0.11 μM. The contact of 1 with fluoride would induce the cleavage of Si-O bond and trigger the emergence of excited state intramolecular proton transfer (ESIPT) process, and then the enol-like emission at 437 nm decreased accompanying with the increase of keto-like tautomerism emission at 550 nm. More importantly, considering the demand of field detection for fluoride in groundwater and combining the function of smartphone to obtain the chroma of photos. The chroma value of the fluorescence color changes from blue to yellow could be conveniently determined through a color recognizer application installed in smartphone. The device can accurately reflect the concentration of fluoride by analyzing the chroma value. The test in actual water samples confirmed that the simple device based on smartphone could be used efficiently for visual, on-site and accurate detection of fluoride in groundwater.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125182DOI Listing
June 2021

The Mechanism of Layer Stacked Clamping (LSC) for Polishing Ultra-Thin Sapphire Wafer.

Micromachines (Basel) 2020 Aug 6;11(8). Epub 2020 Aug 6.

Ultra-Precision Machining Centre, College of Mechanical Engineering, Zhejiang University of Technology, Hangzhou 310014, China.

Double-sides polishing technology has the advantages of high flatness and parallelism, and high polishing efficiency. It is the preferred polishing method for the preparation of ultra-thin sapphire wafer. However, the clamping method is a fundamental problem which is currently difficult to solve. In this paper, a layer stacked clamping (LSC) method of ultra-thin sapphire wafer which was used on double-sides processing was proposed and the clamping mechanism of layer stacked clamping (LSC) was studied. Based on the rough surface contact model of fractal theory, combining the theory of van der Waals force and capillary force, the adhesion model of the rough surfaces was constructed, and the reliability of the model was verified through experiments. Research has found that after displacement between the two surfaces the main force of the adhesion force is capillary force. The capillary force decreases with the increasing of surface roughness, droplet volume, and contact angle. For an ultra-thin sapphire wafer with a diameter of 50.8 mm and a thickness of 0.17 mm, more than 1.4 N of normal adhesion force can be generated through the LSC method. Through the double-sides polishing experiment using the LSC method, an ultra-thin sapphire wafer with an average surface roughness () of 1.52 nm and a flatness (PV) of 0.968 μm was obtained.
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http://dx.doi.org/10.3390/mi11080759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464148PMC
August 2020

Expression Analysis and Functional Characterization of Family Genes Involved in Very-Long-Chain Alkanes Biosynthesis in .

Front Plant Sci 2019 1;10:1389. Epub 2019 Nov 1.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China.

Cuticular wax accumulation and composition affects drought resistance in plants. plants subjected to water deficit and polyethylene glycol treatments resulted in a significant increase in total wax load, in which very-long-chain (VLC) alkanes were more sensitive to these treatments than other wax compounds, implying that VLC alkanes biosynthesis plays a more important role in drought resistance in . () has been reported to encode a core enzyme involved in VLC alkanes biosynthesis in Arabidopsis (), but few corresponding genes are investigated in . Here, we identified eight homologous genes in , namely to , and then analyzed their sequences feature, expression patterns, stress induction, and biochemical activities. These genes had similar protein structure to other reported and -like genes, but displayed closer phylogenetic relationship to the rice genes. They were further found to exhibit various tissue expression patterns after being induced by abiotic stresses. Among them, gene showed extremely high expression in leaves. Heterologous introduction of into the Arabidopsis mutant rescued VLC alkanes biosynthesis. These results indicate that genes are likely to be involved in VLC alkanes biosynthesis of . Taken together, seems to play an explicit and predominant role in VLC alkanes biosynthesis in leaf. Our work provides important clues for further characterizing function of homologous genes in and also an option to improve drought resistance of cereal crops.
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http://dx.doi.org/10.3389/fpls.2019.01389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6838206PMC
November 2019

TaCER1-1A is involved in cuticular wax alkane biosynthesis in hexaploid wheat and responds to plant abiotic stresses.

Plant Cell Environ 2019 11 13;42(11):3077-3091. Epub 2019 Aug 13.

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Yangling, 712100, China.

To protect above-ground plant organs from excessive water loss, their surfaces are coated by waxes. The genes involved in wax formation have been investigated in detail in Arabidopsis but scarcely in crop species. Here, we aimed to isolate and characterize a CER1 enzyme responsible for formation of the very long-chain alkanes present in high concentrations especially during late stages of wheat development. On the basis of comparative wax and transcriptome analyses of various wheat organs, we selected TaCER1-1A as a primary candidate and demonstrated that it was located to the endoplasmic reticulum, the subcellular compartment for wax biosynthesis. A wheat nullisomic-tetrasomic substitution line lacking TaCER1-1A had significantly reduced amounts of C alkane, whereas rice plants overexpressing TaCER1-1A showed substantial increases of C -C alkanes relative to wild type control. Similarly, heterologous expression of TaCER1-1A in Arabidopsis wild type and the cer1 mutant resulted in increased levels of unbranched alkanes, iso-branched alkanes and alkenes. Finally, the expression of TaCER1-1A was found activated by abiotic stresses and abscisic acid treatment, resulting in increased production of alkanes in wheat. Taken together, our results demonstrate that TaCER1-1A plays an important role in wheat wax alkane biosynthesis and involved in responding to drought and other environmental stresses.
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http://dx.doi.org/10.1111/pce.13614DOI Listing
November 2019

Non-Destructive Determination of Alkylresorcinol (ARs) Content on Wheat Seed Surfaces and Prediction of ARs Content in Whole-Grain Flour.

Molecules 2019 Apr 4;24(7). Epub 2019 Apr 4.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A & F University, Yangling 712100, China.

Alkylresorcinols (ARs) are beneficial for human health and can be used as biomarkers for whole-grain cereal intake. In previous studies, ARs content in whole-grain flour was determined by milling the seeds into powder, thus destroying their structure. In this paper, we adopted a non-destructive extraction approach. Chloroform and ethyl acetate extraction methods were carried out to extract lipids from the seed surface and whole-grain flour of 36 wheat varieties in China, respectively. GC-MS analysis identified chemical compounds in which ARs were the major compounds for all the samples. The average total content of ARs (624.223 µg/g) in whole grains was much higher than that on seed surfaces (4.934 µg/g), with a positive correlation (r = 0.863, < 0.001) between these two parameters. The results suggested that the total ARs content on the seed surface can be used to predict their content in whole-grain flour. Without affecting the seed germination rate and damaging seed structure, we demonstrate that a non-destructive extraction approach is an appropriate and useful method, facilitating the development of rapid screening methods suitable for use in wheat breeding.
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http://dx.doi.org/10.3390/molecules24071329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479818PMC
April 2019

Knowledge, attitude, and practice of healthcare professionals toward clinically applying graduated compression stockings: results of a Chinese web-based survey.

J Thromb Thrombolysis 2019 Jan;47(1):102-108

Department of Orthopedics, China-Japan Friendship Hospital, Beijing, China.

This study had three objectives: (1) to investigate healthcare professionals' knowledge, attitude, and practice toward clinically applying graduated compression stockings, (2) to analyze the influencing factors, and (3) to provide data to support departments that develop health policies. A self-administered web-based survey was completed by 1,444 healthcare professionals, including physicians and nurses from 15tertiary hospitals and four secondary hospitals from 10 provinces in China. Reliability analysis and exploratory factor analysis were used to evaluate the researcher-designed questionnaire's reliability and validity. The formal questionnaire, which included demographic data (eight items), knowledge (ten items), attitudes (four items), and clinical practice patterns (six items), was distributed among healthcare professionals. The relationships and mechanisms among the variables were explored using descriptive statistical analysis, Pearson's correlation coefficients, and multiple linear regression analysis. Of the 1,444 respondents, 31.2% had good knowledge of clinically applying graduated compression stockings, 83.5% had a positive attitude toward clinically applying them, and 30.4% of respondents exhibited normative behavior when applying them. The multiple linear regression analysis indicated that training was an important factor influencing the knowledge, attitude, and practice toward clinically applying graduated compression stockings. The healthcare professionals' attitude toward clinically applying graduated compression stockings was positive, but the related knowledge was poor, and the code of behavior was deficient. Medical institutions should improve training for clinically applying graduated compression stockings among healthcare professionals and standardize the use of graduated compression stockings to facilitate the prevention of hospital-acquired venous thromboembolism.
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http://dx.doi.org/10.1007/s11239-018-1749-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336747PMC
January 2019

Two transcription factors TaPpm1 and TaPpb1 co-regulate anthocyanin biosynthesis in purple pericarps of wheat.

J Exp Bot 2018 04;69(10):2555-2567

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China.

Purple pericarps of bread wheat (Triticum aestivum L.) are a useful source of dietary anthocyanins. Previous mapping results indicated that the purple pericarp trait is controlled by two complementary genes located on chromosomes 7D and 2A. However, the identity of the genes and the mechanisms by which they regulate the trait are unknown. In this study, two transcription factors were characterised as anthocyanin activators in purple pericarps: TaPpm1 (purple pericarp-MYB 1) and TaPpb1 (purple pericarp-bHLH 1). Three non-functional variants were detected in the coding sequence of TaPpm1 from non-purple seed lines, in which the function of TaPpm1 was destroyed either by insertion-induced frame shifts or truncated peptides. There were six 261-bp tandem repeats in the promoter region of TaPpb1 in the purple-grained varieties, while there was only one repeat unit present in the non-purple varieties. Furthermore, using yeast two-hybrid, dual luciferase, yeast one-hybrid, and transient assays, we were able to demonstrate that the interaction of TaPpm1 and TaPpb1 co-regulates the synthesis of anthocyanin. Overall, our results provide a better understanding of the molecular basis of anthocyanin synthesis in the wheat pericarp and indicate the existence of an integrated regulatory mechanism that controls production.
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http://dx.doi.org/10.1093/jxb/ery101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920291PMC
April 2018

Using a Kinect sensor to acquire biological motion: Toolbox and evaluation.

Behav Res Methods 2018 04;50(2):518-529

Department of Psychology and Behavioral Sciences, Xixi Campus, Zhejiang University, Hang Zhou, People's Republic of China, 310028.

Biological motion (BM) is the movement of animate entities, which conveys rich social information. To obtain pure BM, researchers nowadays predominantly use point-light displays (PLDs), which depict BM through a set of light points (e.g., 12 points) placed at distinct joints of a moving human body. Most prevalent BM stimuli are created by state-of-the-art motion capture systems. Although these stimuli are highly precise, the motion capture system is expensive and bulky, and its process of constructing a PLD-based BM is time-consuming and complex. These factors impede the investigation of BM mechanisms. In this study, we propose a free Kinect-based biological motion capture (KBC) toolbox based on the Kinect Sensor 2.0 in C++. The KBC toolbox aims to help researchers acquire PLD-based BM in an easy, low-cost, and user-friendly way. We conducted three experiments to examine whether KBC-generated BM can genuinely reflect the processing characteristics of BM: (1) Is BM from this source processed globally in vision? (2) Does its BM (e.g., from the feet) retain detailed local information? and (3) Does the BM convey emotional information? We obtained positive results in response to all three questions. Therefore, we think that the KBC toolbox can be useful in generating BM for future research.
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http://dx.doi.org/10.3758/s13428-017-0883-9DOI Listing
April 2018

Developmental Changes in Composition and Morphology of Cuticular Waxes on Leaves and Spikes of Glossy and Glaucous Wheat (Triticum aestivum L.).

PLoS One 2015 27;10(10):e0141239. Epub 2015 Oct 27.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China.

The glossy varieties (A14 and Jing 2001) and glaucous varieties (Fanmai 5 and Shanken 99) of wheat (Triticum aestivum L.) were selected for evaluation of developmental changes in the composition and morphology of cuticular waxes on leaves and spikes. The results provide us with two different wax development patterns between leaf and spike. The general accumulation trend of the total wax load on leaf and spike surfaces is first to increase and then decrease during the development growth period, but these changes were caused by different compound classes between leaf and spike. Developmental changes of leaf waxes were mainly the result of variations in composition of alcohols and alkanes. In addition, diketones were the third important contributor to the leaf wax changes in the glaucous varieties. Alkanes and diketones were the two major compound classes that caused the developmental changes of spike waxes. For leaf waxes, β- and OH-β-diketones were first detected in flag leaves from 200-day-old plants, and the amounts of β- and OH-β-diketones were significantly higher in glaucous varieties compared with glossy varieties. In spike waxes, β-diketone existed in all varieties, but OH-β-diketone was detectable only in the glaucous varieties. Unexpectedly, the glaucous variety Fanmai 5 yielded large amounts of OH-β-diketone. There was a significant shift in the chain length distribution of alkanes between early stage leaf and flag leaf. Unlike C28 alcohol being the dominant chain length in leaf waxes, the dominant alcohol chain length of spikes was C24 or C26 depending on varieties. Epicuticular wax crystals on wheat leaf and glume were comprised of platelets and tubules, and the crystal morphology changed constantly throughout plant growth, especially the abaxial leaf crystals. Moreover, our results suggested that platelets and tubules on glume surfaces could be formed rapidly within a few days.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0141239PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624236PMC
June 2016
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