Publications by authors named "Jiafei Yang"

4 Publications

  • Page 1 of 1

The relationship between abnormal Core binding factor-β expression in human cartilage and osteoarthritis.

BMC Musculoskelet Disord 2021 Feb 11;22(1):174. Epub 2021 Feb 11.

Department of Orthopaedics, The Affiliated Hospital of Guizhou Medical University, Guiyang, China.

Background: This study aimed to investigate the effect of abnormal Core binding factor-β expression on proliferation, differentiation and apoptosis of chondrocytes, and elucidate the relationship between Core binding factor-β and osteoarthritis-related markers and degenerative joint disease.

Methods: Cartilage tissues, from healthy subjects and patients with osteoarthritis, were collected for histology and expression of Core binding factor-β, MMP-13, IL-1β, COMP, and YKL-40. Human articular chondrocytes were cultured in vitro, and a viral vector was constructed to regulate cellular Core binding factor-β expression. Cellular proliferation and apoptosis were observed, and osteoarthritis-related inflammatory factor expression and cartilage metabolite synthesis assayed.

Results: Human osteoarthritis lesions had disordered cartilage structure and cellular arrangement, and increased emptying of cartilage lacunae. Normal cell counts were significantly reduced, cartilage extracellular matrix was obviously damaged, and type II collagen expression was significantly decreased. Core binding factor-β was highly expressed in the osteoarthritis cartilage (p < 0.001), and MMP-13, IL-1β, COMP and YKL-40 expression were greater than found in normal cartilage (p < 0.001). Cellular proliferation in the Core binding factor-β high-expression group was reduced and the total apoptosis rate was increased (p < 0.05), while the opposite was found in the Core binding factor-β inhibition group (p < 0.01). Compared with normal chondrocytes, high Core binding factor-β expression (Osteoarthritis and CBFB/pCDH groups) was associated with significantly increased MMP13, IL-1β, COMP and YKL-40 protein expression (p < 0.01), while Core binding factor-β inhibition (CBFB/pLKO.1 group) was associated with significantly decreased COMP, MMP13, IL-1β and YKL-40 expression in osteoarthritis cells (p < 0.001).

Conclusions: Abnormal Core binding factor-β expression might play an upstream regulatory role in mediating abnormal chondrocyte apoptosis and the inflammatory response. On inhibiting Core binding factor-β expression, a delay in cartilage degeneration was expected.

Trial Registration: The study was registered for clinical trials in ChiCTR: ChiCTR1800017066 (Reg. Date-2018/7/10).
View Article and Find Full Text PDF

Download full-text PDF

Source Listing
February 2021

Association of Age and Size of Carotid Artery Intraplaque Hemorrhage and Minor Fibrous Cap Disruption: A High Resolution Magnetic Resonance Imaging Study.

J Atheroscler Thromb 2018 Dec 17;25(12):1222-1230. Epub 2018 Apr 17.

Center for Biomedical Imaging Research, Department of Biomedical Engineering, Tsinghua University School of Medicine.

Aim: To investigate the association between the volumes of different aging intraplaque hemorrhage (IPH) and minor fibrous cap disruption (MFCD) in carotid arteries.

Methods: Patients with cerebrovascular symptoms and carotid atherosclerotic plaques determined by ultrasound were recruited and underwent multi-contrast magnetic resonance (MR) vessel wall imaging for carotid arteries. Carotid plaques with IPH on MR imaging were included in the analysis. The age (fresh or recent) and the volume of IPH for each plaque were evaluated.

Results: In total, 41 carotid plaques in 37 patients (mean age 70.2±11.0 years old; 32 males) were eligible for statistical analysis. The absolute volume of fresh IPH in plaques with MFCD was significantly larger than that in plaques without MFCD (109.83±75.49 mm vs. 30.54±20.62 mm, P=0.002). Logistic regression showed that the absolute volume of fresh IPH was significantly associated with MFCD before (odds ratio [OR], 1.735; 95% confidence interval [CI], 1.127-2.670; P=0.012) and after adjusting for confounding factors (OR, 1.823; 95% CI, 1.076-3.090; P=0.026). There was no significant association between recent IPH volume and MFCD (P>0.05).

Conclusion: The volume of fresh IPH is independently associated with MFCD in carotid plaques, suggesting that integrity of fibrous cap may change with different age and size of IPH.
View Article and Find Full Text PDF

Download full-text PDF

Source Listing
December 2018

Kalman Filtered Bio Heat Transfer Model Based Self-adaptive Hybrid Magnetic Resonance Thermometry.

IEEE Trans Med Imaging 2017 01 18;36(1):194-202. Epub 2016 Aug 18.

To develop a self-adaptive and fast thermometry method by combining the original hybrid magnetic resonance thermometry method and the bio heat transfer equation (BHTE) model. The proposed Kalman filtered Bio Heat Transfer Model Based Self-adaptive Hybrid Magnetic Resonance Thermometry, abbreviated as KalBHT hybrid method, introduced the BHTE model to synthesize a window on the regularization term of the hybrid algorithm, which leads to a self-adaptive regularization both spatially and temporally with change of temperature. Further, to decrease the sensitivity to accuracy of the BHTE model, Kalman filter is utilized to update the window at each iteration time. To investigate the effect of the proposed model, computer heating simulation, phantom microwave heating experiment and dynamic in-vivo model validation of liver and thoracic tumor were conducted in this study. The heating simulation indicates that the KalBHT hybrid algorithm achieves more accurate results without adjusting λ to a proper value in comparison to the hybrid algorithm. The results of the phantom heating experiment illustrate that the proposed model is able to follow temperature changes in the presence of motion and the temperature estimated also shows less noise in the background and surrounding the hot spot. The dynamic in-vivo model validation with heating simulation demonstrates that the proposed model has a higher convergence rate, more robustness to susceptibility problem surrounding the hot spot and more accuracy of temperature estimation. In the healthy liver experiment with heating simulation, the RMSE of the hot spot of the proposed model is reduced to about 50% compared to the RMSE of the original hybrid model and the convergence time becomes only about one fifth of the hybrid model. The proposed model is able to improve the accuracy of the original hybrid algorithm and accelerate the convergence rate of MR temperature estimation.
View Article and Find Full Text PDF

Download full-text PDF

Source Listing
January 2017

Fast temperature estimation from undersampled k-space with fully-sampled center for MR guided microwave ablation.

Magn Reson Imaging 2016 Oct 20;34(8):1171-80. Epub 2016 May 20.

Key Laboratory of Particle and Radiation Imaging, Ministry of Education, Medical Physics and Engineering Institute, Department of Engineering Physics, Tsinghua University, Beijing, China. Electronic address:

Purpose: This study aims to accelerate MR temperature imaging using the proton resonance frequency (PRF) shift method for real time temperature monitoring during thermal ablation.

Materials And Methods: The proposed method estimates temperature changes from undersampled k-space with a fully sampled center. This proposed algorithm is based on the hybrid multi-baseline and referenceless treatment image model and can be seen as an extension of the conventional k-space-based hybrid thermometry. The parameters of hybrid model are acquired by utilizing information from low resolution images which are obtained from fully-sampled centers of k-space. Registration is used to correct temperature errors due to the displacement of the subject. Phantom heating simulations, motion simulations, phantom heating and in-vivo experiments were performed to investigate the efficiency of the proposed method. SPIRiT and the conventional k-space estimation reconstruction thermometry were implemented for comparison using the same sampling pattern.

Results: The phantom heating simulations showed that the proposed method results in lower RMSEs than the conventional k-space hybrid thermometry and SPIRiT at various reduction factors tested. The motion simulations indicated the robustness of the proposed method to displacement of the subject. Phantom heating experiment further demonstrated the ability of the method to reconstruct temperature maps with less computation time and higher accuracy (RMSEs lower than 0.4°C) at a net reduction factor of 3.5 in the presence of large noise caused by a microwave needle. In-vivo experiments validated the feasibility of the proposed method to estimate temperature changes from undersampled k-space (net reduction factor 4.3) in presence of respiratory motion and complicated anatomical structure, while reducing computation time as much as 10-fold compared with the conventional k-space method.

Conclusion: The proposed method accelerates the PRF-shift MR thermometry and provides more accurate temperature maps in presence of motion with relatively short computation time, which may make real time imaging for MR-guided microwave ablation possible.
View Article and Find Full Text PDF

Download full-text PDF

Source Listing
October 2016