Publications by authors named "Jiadong Li"

38 Publications

An l-rhamnose-binding lectin from Nile tilapia (Oreochromis niloticus) possesses agglutination activity and regulates inflammation, phagocytosis and respiratory burst of monocytes/macrophages.

Dev Comp Immunol 2021 Sep 11;126:104256. Epub 2021 Sep 11.

Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangdong Provincial Engineering Technology Research Center for Environmentally-Friendly Aquaculture, School of Life Sciences, South China Normal University, Guangzhou, 510631, PR China. Electronic address:

Rhamnose-binding lectins (RBLs), a Ca-independent lectin family, are widely present in vertebrates and invertebrates, which involve in the innate immune response. However, the functional characterization and related regulation mechanisms of RBLs remain unclear in teleost fish. In this study, an l-rhamnose-binding lectin-like (OnRBL-L) was identified and functionally characterized from Nile tilapia (Oreochromis niloticus). The open reading frame of OnRBL-L is 678 bp encoding 225 aa. The sequence of OnRBL-L has relatively conservative characteristic peptide motifs, including YGR, DPC, and KYL-motif. Expression analysis showed that OnRBL-L was abundantly distributed in intestine tissue, and widely existed in all detected tissues. Meanwhile, the expression of OnRBL-L increased significantly in vivo (liver, spleen, head kidney, intestine, gills and peripheral blood) and in vitro (monocytes/macrophages) following challenges with two important tilapia pathogenic bacteria Streptococcus agalactiae and Aeromonas hydrophila. In addition, the recombinant OnRBL-L was found to bind and agglutinate S. agalactiae and A. hydrophila. Furthermore, OnRBL-L could participate in non-specific cellular immune defense, including reducing the expression of pro-inflammatory factors (IL-6、IL-8 and TNF-α), and enhancement of the phagocytosis and respiratory burst of MO/MФ. Overall, our results provide new insights into the understanding of RBL as an important pattern recognition molecule and regulator in non-specific cell immunity in an early vertebrate.
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http://dx.doi.org/10.1016/j.dci.2021.104256DOI Listing
September 2021

Gaussian fitting algorithm with multi-geometric parameters for rotated elliptical beam profiling using pixel ion chamber.

Med Phys 2021 Sep 11;48(9):4799-4811. Epub 2021 Aug 11.

State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Hubei, China.

Purpose: A high-precision rotated elliptical beam profiling method based on pixel ion chamber is proposed in this paper. This method aims to improve the accuracy by modeling the transverse profile of rotated beam as an ellipse with additional correlation coefficient and eliminating the fitting error due to the volume averaging effect of pixel ion chamber.

Methods: In pencil beam scanning (PBS) proton therapy systems, the transverse beam profile model is generally represented as a standard Gaussian distribution. Considering the elliptical spots, two-dimensional (2D) joint Gaussian distribution characterized with the correlation coefficient ρ is adopted in this study. Gaussian-type particle distribution with white noise was generated and processed in MATLAB to simulate the secondary particle collection in the pixel ion chamber. The simulated pixel ion chamber is a commercially available ion chamber which consists of 12 × 12 small square pixels (3.75 × 3.75 mm ) with a 0.05 mm interval. The simulated signals were preprocessed by filtering with the noise threshold and extracting the maximum simply connected domain (MSCD) of the signal. Then, five geometric parameters that identify the transverse beam profiles were fitted under different signal-to-noise ratio (SNR) conditions: the center of the beam (x , y ), the spot size (σ , σ ), and the rotation angle θ formed between the major axes of elliptical spot and the x axes of the ion chamber. First, the simulated signals were preprocessed by filtering with the noise threshold and extracting the MSCD of the signal. Second, a rectification curve of systematic error in fitted spot size versus the prescribed spot size was used to predict the systematic error due to the volume averaging effect. Finally, the effects of fitting errors on therapeutic dose were evaluated in terms of gamma index and relative dose difference.

Results: When the SNR is not less than 20 dB, the relative fitting error of spot size and the absolute fitting error of angle θ are less than 1% and 6.1°, respectively. The fitting error of beam center increases with spot size and will not exceed 0.22 mm when spot size reaches up to 12 mm. At a SNR equal to 20 dB, neither cold nor hot spots were presented in dose distribution calculated with the fitted spot parameters.

Conclusion: The improved Gaussian fitting algorithm performs well when SNR is not less than 20 dB. This method can effectively distinguish the nominal beam and rotated elliptical beam. An ideal systematic error curve can be predicted and used to correct the fitted spot size, thus eliminating the systematic error due to the volume averaging effect of the pixel ion chamber. The fitting error of spot size cannot be fully corrected, but it is negligible and shows little effect on the overall therapeutic dose.
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http://dx.doi.org/10.1002/mp.15140DOI Listing
September 2021

Enhanced Secretory Expression and Surface Display Level of Bombyx mori Acetylcholinesterase 2 by Pichia pastoris Based on Codon Optimization Strategy for Pesticides Setection.

Appl Biochem Biotechnol 2021 Jun 23. Epub 2021 Jun 23.

Guangdong Provincial Key Laboratory of Food Quality and Safety, National-Local Joint Engineering Research Center for Processing and Safety Control of Livestock and Poultry Products, College of Food Science, South China Agricultural University, Guangzhou, 510642, People's Republic of China.

The cholinesterase-based spectrophotometric assay, also called enzyme inhibition method, is a good choice for rapid detection of organophosphate pesticides (OPs) and carbamate pesticides (CPs). Obviously, the cholinesterase is the core reagent in enzyme inhibition method. In our previous work, a recombinant acetylcholinesterase 2 from Bombyx mori (rBmAChE2) was expressed in yeast successfully and exhibited great sensitivity. However, the yield of rBmAChE2 is not desirable. In this study, a codon optimization strategy was employed to enhance the yield of rBmAChE2 in Pichia pastoris GS115. Results showed that by replacing 6 key rare codons and increasing the percentage of bases G and C up to 46.85%, codon adaptation index (CAI) of Bombyx mori acetylcholinesterase 2 (bmace2) gene was improved from 0.70 to 0.81. After being transformed into Pichia pastoris GS115 via electroporation, the expression transformant can produce 139.7 U/mL secretory codon-optimized rBmAChE2 (opt-rBmAChE2) in the culture supernatant, 3.62 times higher than that of strain bearing the wild-type bmace2 gene. Meanwhile, opt-rBmAChE2 displayed on the yeast surface was up to 2280.02 U/g, 2.8 times higher than wild-type displayed rBmAChE2. In addition, either secretory or surface-displayed opt-rBmAChE2 maintained the similar sensitivities to the wild-type rBmAChE2 for tested inhibitors. Furthermore, the detection limits of the opt-rBmAChE2-based enzyme inhibition method for 10 kinds of OPs or CPs (0.01-2.69 mg/kg) were lower than most of the indexes present in current standard method (GB/T 5009.199-2003) or the maximum residue limits (GB 2763-2019) in China. The results might contribute to the utilization of rBmAChE2 for pesticide residue screening detection in practice.
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http://dx.doi.org/10.1007/s12010-021-03597-7DOI Listing
June 2021

Correction: An electronic enzyme-linked immunosorbent assay platform for protein analysis based on magnetic beads and AlGaN/GaN high electron mobility transistors.

Analyst 2021 Jun;146(13):4400

Key Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, People's Republic of China. and School of Nano Technology and Nano Bionics, University of Science and Technology of China, Hefei, 230026, People's Republic of China.

Correction for 'An electronic enzyme-linked immunosorbent assay platform for protein analysis based on magnetic beads and AlGaN/GaN high electron mobility transistors' by Jin Wang et al., Analyst, 2020, 145, 2725-2730, DOI: 10.1039/C9AN01809C.
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http://dx.doi.org/10.1039/d1an90048jDOI Listing
June 2021

An enhanced open sandwich immunoassay by molecular evolution for noncompetitive detection of Alternaria mycotoxin tenuazonic acid.

Food Chem 2021 Nov 13;361:130103. Epub 2021 May 13.

Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China. Electronic address:

Open sandwich enzyme-linked immunosorbent assay (OS-ELISA), a novel noncompetitive immunoassay format, has shown great potential in rapid detection for small molecules compared with traditional competitive format. Here, an enhanced OS-ELISA towards the mycotoxin tenuazonic acid (TeA) was developed for the first time based on heavy chain variable region (V) and light chain variable region (V) from the hybridoma cells (3F10) producing anti-TeA monoclonal antibody (mAb). The established OS-ELISA exhibited a limit of detection of 0.08 ng/mL, and was 13 times more sensitive than mAb-based indirect competitive ELISA (ic-ELISA). The proposed assay was also applied to detect TeA contents in juice, flour and tomato ketchup samples with satisfactory recoveries of 87.6%-111.3%. Finally, the great accuracy of the established OS-ELISA method was validated by the standard ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS).
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http://dx.doi.org/10.1016/j.foodchem.2021.130103DOI Listing
November 2021

Preparation of a Bombyx mori acetylcholinesterase enzyme reagent through chaperone protein disulfide isomerase co-expression strategy in Pichia pastoris for detection of pesticides.

Enzyme Microb Technol 2021 Mar 31;144:109741. Epub 2020 Dec 31.

Guangdong Provincial Key Laboratory of Food Quality and Safety, National-Local Joint Engineering Research Center for Processing and Safety Control of Livestock and Poultry Products, College of Food Science, South China Agricultural University, Guangzhou, 510642, PR China. Electronic address:

The cholinesterase-based spectrophotometric methods for detection of organophosphate pesticides (OPs) and carbamate pesticides (CPs) have been proposed as a good choice for their high efficiency, simplicity and low cost. The enzyme, as a core reagent, is of great importance for the developed method. In this study, a protein disulfide isomerase (PDI) co-expression strategy in Pichia pastoris was employed to enhance the yield of recombinant Bombyx mori acetylcholinesterase 2 (rBmAChE2). Subsequently, the prepared enzyme reagent was used to detect the pesticides in real samples. The results showed that the co-expression of rBmAChE2 with PDI increased the enzyme activity of the supernatant and the yield of purified rBmAChE2 up to 60 U/mL and 6 mg/L respectively, both almost 5-fold higher than those of original recombinant strain. In addition, 5 g/L gelatin reagent could help to preserve nearly 90% of the rBmAChE2 activity for 90 days in 4°C and the limits of detections (LODs) of the rBmAChE2-based assay for 20 kinds of OPs or CPs ranged from 0.010 to 2.725 mg/kg, which were lower than most of indexes present in current Chinese National Standard (GB/T 5009.199-2003) or the maximum residue limits (GB 2763-2019). Furthermore, the detection results of 23 vegetable samples were verified by the ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method, which indicated that the rBmAChE2-based assay in this work is suitable for pesticide residues rapid detection.
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http://dx.doi.org/10.1016/j.enzmictec.2020.109741DOI Listing
March 2021

Recoverable peroxidase-like [email protected] nanozyme with enhanced antibacterial ability.

Chem Eng J 2021 Mar 6;408:127240. Epub 2020 Oct 6.

State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

Antibacterial agents with enzyme-like properties and bacteria-binding ability have provided an alternative method to efficiently disinfect drug-resistance microorganism. Herein, a [email protected] nanozyme with defect-rich rough surface was constructed by a simple hydrothermal method and photodeposition of Ag nanoparticles. The nanozyme exhibited good antibacterial performance against (~69.4%) by the generated ROS and released Ag, while the nanozyme could further achieve an excellent synergistic disinfection (~100%) by combining with the near-infrared photothermal property of [email protected] The antibacterial mechanism study showed that the antibacterial process was determined by the collaborative work of peroxidase-like activity, photothermal effect and leakage of Ag. The defect-rich rough surface of MoS layers facilitated the capture of bacteria, which enhanced the accurate and rapid attack of •OH and Ag to the membrane of with the assistance of local hyperthermia. This method showed broad-spectrum antibacterial performance against Gram-negative bacteria, Gram-positive bacteria, drug-resistant bacteria and fungal bacteria. Meanwhile, the magnetism of FeO was used to recycle the nanozyme. This work showed great potential of engineered nanozymes for efficient disinfection treatment.
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http://dx.doi.org/10.1016/j.cej.2020.127240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7536174PMC
March 2021

Higher expression of miR-150-5p promotes tumorigenesis by suppressing LKB1 in non-small cell lung cancer.

Pathol Res Pract 2020 Nov 1;216(11):153145. Epub 2020 Aug 1.

Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai 200444, China. Electronic address:

Lung cancer is one of the most malignant tumors that can form in the human. MicroRNAs (MiRNAs) play significant role in tumor progression. Human lung cancer tissues and cell lines were used to determine miR-150-5p respectively, and Liver Kinase B1 (LKB1) expression using quantitative real-time PCR (qRT-PCR). The data analysis website Kaplan-Meier Plotter (database obtained from The Cancer Genome Atlas) was used to perform a survival analysis with LKB1 levels. Using the appropriate assays, the function of miR-150-5p was also detected in cellular proliferation, migration and cell apoptosis as well as cell cycle. Results revealed that miR-150-5p was upregulated in non-small cell lung cancer (NSCLC) tissue and cell lines. In NSCLC, miR-150-5p promoted cellular proliferation and migration, but decreased cellular apoptosis. Conversely, miR-150-5p inhibition suppressed cellular growth. These results further revealed a network of cellular signaling for miR-150-5p to target LKB1. Ectopic expression of LKB1 can mitigate the tumor-promoting function of miR-150-5p. Collectively, these results indicated that miR-150-5p may promote lung cancer by inhibiting the suppressor gene LKB1 in NSCLC.
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http://dx.doi.org/10.1016/j.prp.2020.153145DOI Listing
November 2020

A Novel Approach to Improve the Microstructure and Mechanical Properties of Al-Mg-Si Aluminum Alloys during Twin-Roll Casting.

Materials (Basel) 2020 Apr 6;13(7). Epub 2020 Apr 6.

Key Laboratory of Electromagnetic Processing of Materials, Ministry of Education, Northeastern University, Shenyang 110819, China.

The main purpose of this present study was to investigate the different processing conditions on the microstructure, segregation behavior of alloying elements, and mechanical properties of Al-Mg-Si alloy twin-roll cast slab prepared using a novel twin-roll casting technology. The simulation of temperature field, distribution of alloying elements, tensile properties, hardness, and conductivity were examined by a Leica optical microscope, scanning electron microscopy, energy dispersion spectroscopy, electron probe microanalysis, and tensile tests. The results indicated that when the traditional twin-roll casting method was used to produce aluminum alloy strip, there are obvious centerline segregation defects due to the deep crystallization front depth and symmetrical solidification characteristics. When the forced-cooling technology was applied in the twin-roll casting process, by virtue of the changing of crystallization front depth and crystallization front shape, the segregation defects are obviously suppressed. Suggesting that this method can significantly improve the uniformity of alloying elements in the thickness direction of the twin-roll cast slab, ultimately improve the mechanical properties of AA6022 aluminum alloy.
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http://dx.doi.org/10.3390/ma13071713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178634PMC
April 2020

An electronic enzyme-linked immunosorbent assay platform for protein analysis based on magnetic beads and AlGaN/GaN high electron mobility transistors.

Analyst 2020 Apr 20;145(7):2725-2730. Epub 2020 Feb 20.

Key Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, People's Republic of China.

AlGaN/GaN high electron mobility transistor (HEMT) biosensors have attracted attention due to their high sensitivity, stability, and fast response characteristics. Some related studies have been explored but a Debye screening problem exists in physiological solutions hindering the detection of bio-macromolecules. Herein, a novel fast analytical platform for electronic enzyme-linked immunosorbent assay (e-ELISA) is proposed based on AlGaN/GaN HEMT with magnetic beads (MBs); MB-based e-ELISA decouples the modified area from the sensing surface to simplify the assay. Combining the advantages of e-ELISA and MBs, the resulting analytical platform presents a sensing capability beyond the Debye-screening limit and a novel ability to be reused. This platform offers a fast response toward prostate specific antigen (PSA) and the lowest concentration of detection is 1 fg mL. Compared with conventional AlGaN/GaN HEMT biosensors, it shows higher sensitivity (3.73 μA dec) in a linear range (1 fg mL to 1 pg mL), which is within the constraints of emergency care applications. The platform's high sensitivity and fast repeatability endow it with great potential for early and rapid diagnosis.
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http://dx.doi.org/10.1039/c9an01809cDOI Listing
April 2020

High-throughput long paired-end sequencing of a Fosmid library by PacBio.

Plant Methods 2019 26;15:142. Epub 2019 Nov 26.

1College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 China.

Background: Large insert paired-end sequencing technologies are important tools for assembling genomes, delineating associated breakpoints and detecting structural rearrangements. To facilitate the comprehensive detection of inter- and intra-chromosomal structural rearrangements or variants (SVs) and complex genome assembly with long repeats and segmental duplications, we developed a new method based on single-molecule real-time synthesis sequencing technology for generating long paired-end sequences of large insert DNA libraries.

Results: A Fosmid vector, pHZAUFOS3, was developed with the following new features: (1) two 18-bp non-palindromic ISceI sites flank the cloning site, and another two sites are present in the skeleton of the vector, allowing long DNA inserts (and the long paired-ends in this paper) to be recovered as single fragments and the vector (~ 8 kb) to be fragmented into 2-3 kb fragments by I-SceI digestion and therefore was effectively removed from the long paired-ends (5-10 kb); (2) the (Cm) resistance gene and replicon (V), necessary for colony growth, are located near the two sides of the cloning site, helping to increase the proportion of the paired-end fragments to single-end fragments in the paired-end libraries. Paired-end libraries were constructed by ligating the size-selected, mechanically sheared pooled Fosmid DNA fragments to the (Amp) resistance gene fragment and screening the colonies with Cm and Amp. We tested this method on yeast and Yugu1. Fosmid-size paired-ends with an average length longer than 2 kb for each end were generated. The N50 scaffold lengths of the de novo assemblies of the yeast and Yugu1 genomes were significantly improved. Five large and five small structural rearrangements or assembly errors spanning tens of bp to tens of kb were identified in Yugu1 including deletions, inversions, duplications and translocations.

Conclusions: We developed a new method for long paired-end sequencing of large insert libraries, which can efficiently improve the quality of de novo genome assembly and identify large and small structural rearrangements or assembly errors.
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http://dx.doi.org/10.1186/s13007-019-0525-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6878638PMC
November 2019

Effects of the Quenching Rate on the Microstructure, Mechanical Properties and Paint Bake-Hardening Response of Al-Mg-Si Automotive Sheets.

Materials (Basel) 2019 Oct 31;12(21). Epub 2019 Oct 31.

State Key Laboratory of Rolling and Automation, Northeastern University, Shenyang 110819, China.

The quenching rate of Al-Mg-Si alloys during solution treatment is an important parameter for the automotive industry. In this work, the effect of the different quenching rates on the microstructures, mechanical properties, and paint bake-hardening response of Al-Mg-Si sheets was studied. Large dimples form on the fracture surface of a sample at a quenching rate of 0.01 °C/s. When the quenching rate increased to 58.9 °C/s, the dimples became smaller. The recrystallized grains and textures were slightly affected by quenching rates beyond 1.9 °C/s. Thus, higher values of the samples were achieved with slower quenching rates. Furthermore, only the Al(FeMn)SiCr insoluble phases were observed in samples with a rapid quenching rate. Sufficient solute atoms and vacancies resulted in the improvement of the precipitation kinetics and paint bake-hardening capacity for Al-Mg-Si sheets at rapid rates. With a decrease in the quenching rate, the formation of the rod-like coarse β' phases consumed many solute atoms and vacancies, leading to the deterioration of the paint bake-hardening capacity. This study provides a critical reference on quenching rates for industrial practices, so that good mechanical properties can be achieved using precision control of the quenching process.
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http://dx.doi.org/10.3390/ma12213587DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6861895PMC
October 2019

Plasmonic Ag decorated graphitic carbon nitride sheets with enhanced visible-light response for photocatalytic water disinfection and organic pollutant removal.

Chemosphere 2020 Mar 24;242:125201. Epub 2019 Oct 24.

State Key Laboratory of Urban Water Resource and Environment School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, China. Electronic address:

Photocatalytic disinfection with high performance is thought to be a promising way for water purification. Herein, plasmonic Ag doped urea-derived graphitic carbon nitride (g-CN) composites were fabricated via in-situ photo-deposition at room temperature as the visible-light photocatalyst. Scan electron microscopy and transmission electron microscopy images showed the uniform dispersion of Ag nanoparticles on the surface of g-CN sheet, which facilitated the synergistic effect of antibacterial performance from Ag and photocatalytic property from Ag/g-CN composites. Photocatalytic water disinfection against Escherichia coli with visible light was performed to demonstrate the improved photocatalytic property with assistance of Ag. The 3-Ag/g-CN exhibited the best bactericidal performance by inactivating all bacteria within 120 min with damaged cell membranes of Escherichia coli observed by scan electron microscopy and transmission electron microscopy images. Photoluminescence spectra, steady-state surface photovoltage spectra, photocurrent response, and electrochemical impedance spectra results revealed that Ag nanoparticles inhibited the recombination of photo-generated e and h pairs and further reinforced the photocatalytic performance of g-CN. Scavenger experiments indicated that h produced on valence band of g-CN dominated the photocatalytic disinfection process against Escherichia coli. This work further proved Ag/g-CN showed great potential in photocatalytic water disinfection under visible-light irradiation.
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http://dx.doi.org/10.1016/j.chemosphere.2019.125201DOI Listing
March 2020

Confinement-Enhanced Rapid Interlayer Diffusion within Graphene-Supported Anisotropic ReSe Electrodes.

ACS Appl Mater Interfaces 2019 Aug 14;11(34):31147-31154. Epub 2019 Aug 14.

Department of Chemical and Biological Engineering, William Mong Institute of Nano Science and Technology and Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction , The Hong Kong University of Science and Technology , Clear Water Bay, Kowloon 999077 , Hong Kong.

To enhance interlayer lithium diffusion, we engineer electrodes consisting of epitaxially grown ReSe nanosheets by chemical vapor deposition, supported on three-dimensional (3D) graphene foam, taking advantage of its weak van der Waals coupling and anisotropic crystal structure. We further demonstrate its excellent performance as the anode for lithium-ion battery and catalyst for hydrogen evolution reaction (HER). Density functional theory calculation reveals that ReSe exhibits a low energy barrier for lithium (Li) interlayer diffusion because of negligible interlayer coupling and anisotropic structure with low symmetry that creates additional adsorption sites and leads to a reduced diffusion barrier. Benefitting from these properties, the 3D ReSe/graphene foam electrode displays excellent cycling and rate performance with 99.6% capacity retention after 350 cycles and a capacity of 327 mA h g at the current density of 1000 mA g. Additionally, it has exhibited a high activity for HER, in which an exchange current density of 277.8 μA cm is obtained and only an overpotential of 106 mV is required to achieve a current density of -10 mA cm. Our work provides a fundamental understanding of the interlayer diffusion of Li in transition-metal dichalcogenide (TMD) materials and acts as a new tool for designing a TMD-based catalyst.
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http://dx.doi.org/10.1021/acsami.9b08157DOI Listing
August 2019

Self-Powered Intracellular Drug Delivery by a Biomechanical Energy-Driven Triboelectric Nanogenerator.

Adv Mater 2019 Mar 5;31(12):e1807795. Epub 2019 Feb 5.

Chinese Academy of Sciences (CAS) Center for Excellence in Nanoscience, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing, 100083, P. R. China.

Nondestructive, high-efficiency, and on-demand intracellular drug/biomacromolecule delivery for therapeutic purposes remains a great challenge. Herein, a biomechanical-energy-powered triboelectric nanogenerator (TENG)-driven electroporation system is developed for intracellular drug delivery with high efficiency and minimal cell damage in vitro and in vivo. In the integrated system, a self-powered TENG as a stable voltage pulse source triggers the increase of plasma membrane potential and membrane permeability. Cooperatively, the silicon nanoneedle-array electrode minimizes cellular damage during electroporation via enhancing the localized electrical field at the nanoneedle-cell interface and also decreases plasma membrane fluidity for the enhancement of molecular influx. The integrated system achieves efficient delivery of exogenous materials (small molecules, macromolecules, and siRNA) into different types of cells, including hard-to-transfect primary cells, with delivery efficiency up to 90% and cell viability over 94%. Through simple finger friction or hand slapping of the wearable TENGs, it successfully realizes a transdermal biomolecule delivery with an over threefold depth enhancement in mice. This integrated and self-powered system for active electroporation drug delivery shows great prospect for self-tuning drug delivery and wearable medicine.
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http://dx.doi.org/10.1002/adma.201807795DOI Listing
March 2019

Effects of the Mg/Si Ratio on Microstructure, Mechanical Properties, and Precipitation Behavior of Al⁻Mg⁻Si⁻1.0 wt %-Zn Alloys.

Materials (Basel) 2018 Dec 19;11(12). Epub 2018 Dec 19.

State Key Laboratory of Rolling and Automation, Northeastern University, Shenyang 110819, China.

Aluminum alloys are widely used as first-choice materials for lightweight automotive applications. It is important that an alloy have a balance between strength and formability. In this study, the alloys were melted, cast, hot rolled, and cold rolled into 1 mm-thick sheets. The microstructure, mechanical properties, and precipitation behavior of Al⁻Mg⁻Si⁻1.0 wt %-Zn alloys with Mg/Si ratios of 0.5, 1, and 2 after solution treatment were studied using optical and electron microscopy, a tensile test, the Vickers hardness test, and differential scanning calorimetry. The results showed that a high density and number of Al⁻Fe⁻Si particles were observed in the matrix, thus causing the formation of more homogeneous and smaller recrystallized grains after treatment with the solution. In addition, a higher volume fraction of cube and P-types texture components formed during solution treatment. Also, a high value and excellent deep drawability were achieved in the medium-Mg/Si-ratio alloy. The formation of denser strengthening precipitates led to a better paint-bake hardening effect in comparison with the other two alloys. Furthermore, the precipitation kinetics were enhanced by the addition of Si, and the addition of Zn did not alter the precipitation sequence of the Al⁻Mg⁻Si alloy. The dual-phase strengthening effect was not achieved in the studied alloys during paint-bake treatment at 175 °C.
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http://dx.doi.org/10.3390/ma11122591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316410PMC
December 2018

Electrical detection of trace zinc ions with an extended gate-AlGaN/GaN high electron mobility sensor.

Analyst 2019 Jan;144(2):663-668

The College of Nuclear Technology and Automation Engineering, Chengdu University of Technology, Chengdu 610059, People's Republic of China.

In this report, we have developed a high sensitivity zinc ion (Zn2+) detection method based on a Schiff base functionalized extended gate (EG)-AlGaN/GaN high electron mobility (HEMT) sensor. The complexation reaction between the Schiff base and the zinc ions would cause surface potential change on the extended gate, and achieve the purpose of zinc ion detection. Compared with conventional methods, the Schiff base functionalized EG-AlGaN/GaN high electron mobility sensor showed a rapid response (less than 10 seconds) and the limit of detection (LOD) was 1 fM. At the same time, the real-time detection of zinc ion concentration ranging from 1 fM to 1 μM showed good linearity (R2 = 0.9962). These results indicated that it provides a promising real-time detection method for trace-free zinc ion trace detection.
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http://dx.doi.org/10.1039/c8an01770kDOI Listing
January 2019

Participation of xCT in melanoma cell proliferation in vitro and tumorigenesis in vivo.

Oncogenesis 2018 Nov 14;7(11):86. Epub 2018 Nov 14.

Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, 08901, USA.

Our research group demonstrated that riluzole, an inhibitor of glutamatergic signaling reduced melanoma cell proliferation in vitro and tumor progression in vivo. The underlying mechanisms of riluzole are largely unknown. Microarray analyses on two human melanoma cell lines revealed that riluzole stimulates expression of the cystine-glutamate amino acid antiporter, xCT (SLC7A11). Western immunoblot analysis from cultured human melanoma or normal melanocytic cells showed that xCT was significantly overexpressed in most melanomas, but not normal cells. Studies using human tumor biopsy samples demonstrated that overexpression of xCT was correlated with cancer stage and progression. To further investigate if xCT is involved in melanoma cell growth, we derived several stable clones through transfection of exogenous xCT to melanoma cells that originally showed very low expression of xCT. The elevated xCT expression promoted cell proliferation in vitro and inversely, these melanoma clones showed a dose-dependent decrease in cell proliferation in response to riluzole treatment. Xenograft studies showed that these clones formed very aggressive tumors at a higher rate compared to vector controls. Conversely, treatment of xenograft-bearing animals with riluzole down-regulated xCT expression suggesting that xCT is a molecular target of riluzole. Furthermore, protein lysates from tumor biopsies of patients that participated in a riluzole monotherapy phase II clinical trial showed a reduction in xCT levels in post-treatment specimens from patients with stable disease. Taken together, our results show that xCT may be utilized as a marker to monitor patients undergoing riluzole-based chemotherapies.
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http://dx.doi.org/10.1038/s41389-018-0098-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6234219PMC
November 2018

Guided Evolution of Recombinant Acetylcholinesterase II by Homology Modeling to Change Pesticide Sensitivity.

Int J Mol Sci 2018 Oct 27;19(11). Epub 2018 Oct 27.

College of Food Science, South China Agricultural University, Guangzhou 510642, China.

docking; bottleneck; molecular dynamic simulation; side chain.
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http://dx.doi.org/10.3390/ijms19113366DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274772PMC
October 2018

Molecular gated-AlGaN/GaN high electron mobility transistor for pH detection.

Analyst 2018 Jun;143(12):2784-2789

i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, People's Republic of China.

A molecular gated-AlGaN/GaN high electron mobility transistor has been developed for pH detection. The sensing surface of the sensor was modified with 3-aminopropyltriethoxysilane to provide amphoteric amine groups, which would play the role of receptors for pH detection. On modification with 3-aminopropyltriethoxysilane, the transistor exhibits good chemical stability in hydrochloric acid solution and is sensitive for pH detection. Thus, our molecular gated-AlGaN/GaN high electron mobility transistor acheived good electrical performances such as chemical stability (remained stable in hydrochloric acid solution), good sensitivity (37.17 μA/pH) and low hysteresis. The results indicate a promising future for high-quality sensors for pH detection.
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http://dx.doi.org/10.1039/c8an00032hDOI Listing
June 2018

A phase II trial of riluzole, an antagonist of metabotropic glutamate receptor 1 (GRM1) signaling, in patients with advanced melanoma.

Pigment Cell Melanoma Res 2018 07 10;31(4):534-540. Epub 2018 Apr 10.

Rutgers Cancer Institute of New Jersey/Robert Wood Johnson Medical School, New Brunswick, NJ, USA.

Studies demonstrate that GRM, expressed by >60% of human melanomas, may be a therapeutic target. We performed a phase II trial of 100 mg PO bid of riluzole, an inhibitor of GRM1 signaling, in patients with advanced melanoma with the primary endpoint of response rate. Thirteen patients with GRM1-positive tumors were enrolled. No objective responses were observed, and accrual was stopped. Stable disease was noted in six (46%) patients, with one patient on study for 42 weeks. Riluzole was well tolerated, with fatigue (62%) as the most common adverse event. Downregulation of MAPK and PI3K/AKT was noted in 33% of paired tumor biopsies. Hypothesis-generating correlative studies suggested that downregulation of angiogenic markers and increased leukocytes at the active edge of tumor correlate with clinical benefit. Pharmacokinetic analysis showed interpatient variability consistent with prior riluzole studies. Future investigations should interrogate mechanisms of biologic activity and advance the development of agents with improved bioavailability.
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http://dx.doi.org/10.1111/pcmr.12694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013351PMC
July 2018

[Strategies to improve the folding and modification of recombinant proteins: a review].

Sheng Wu Gong Cheng Xue Bao 2017 Apr;33(4):591-600

National-Local Joint Engineering Research Center for Processing and Safety Control of Livestock and Poultry Products (Guangdong), Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, Guangdong, China.

Gene expression technology has made great progress with the continuous developments and researches of molecular biology. Though many systems to produce recombinant proteins have been studied, none of them is available so far to satisfy the needs completely. With the increasing demands of bioactive peptides and protein drugs, expression quantity and correct posttranslational folding and modifications are also needed under the circumstance which can make proteins more close to native conformation and higher activity and stability. Based on our previous work, we summarized the factors affecting the folding and modifications of recombinant proteins correctly from five aspects, including expression system and hosts, secretory expression, coexpression, fusion expression, and the culture conditions, as well as improvement strategies.
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http://dx.doi.org/10.13345/j.cjb.160327DOI Listing
April 2017

Hierarchical NiCoO Micro- and Nanostructures with Tunable Morphologies as Anode Materials for Lithium- and Sodium-Ion Batteries.

ACS Appl Mater Interfaces 2017 May 4;9(19):16194-16201. Epub 2017 May 4.

Department of Chemical and Biomolecular Engineering, The Hong Kong University of Science and Technology , Kowloon, Hong Kong, P.R. China.

NiCoO microrods with open structures are successfully synthesized using a solvothermal method. Compared with those of dense microspheres, the one-dimensional (1D) porous microrods show much higher capacities and stability for both Li- and Na-ion batteries due to the 1D open structure facilitating fast ion transport and buffering volumetric change during charge/discharge. This work demonstrates that the electrochemical performance of NiCoO is highly dependent on morphologies of the active material.
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http://dx.doi.org/10.1021/acsami.7b02175DOI Listing
May 2017

Targeting Glutamatergic Signaling and the PI3 Kinase Pathway to Halt Melanoma Progression.

Transl Oncol 2015 Feb;8(1):1-9

Department of Surgery, Division of Surgical Oncology, Cancer Institute of New Jersey, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ, USA. Electronic address:

Our group has previously reported that the majority of human melanomas (>60%) express the metabotropic glutamate receptor 1 (GRM1) and that the glutamate release inhibitor riluzole, a drug currently used to treat amyotrophic lateral sclerosis, can induce apoptosis in GRM1-expressing melanoma cells. Our group previously reported that in vitro riluzole treatment reduces cell growth in three-dimensional (3D) soft agar colony assays by 80% in cells with wildtype phosphoinositide 3-kinase (PI3K) pathway activation. However, melanoma cell lines harboring constitutive activating mutations of the PI3K pathway (PTEN and NRAS mutations) showed only a 35% to 40% decrease in colony formation in soft agar in the presence of riluzole. In this study, we have continued our preclinical studies of riluzole and its effect on melanoma cells alone and in combination with inhibitors of the PI3 kinase pathway: the AKT inhibitor, API-2, and the mammalian target of rapamycin (mTOR) inhibitor, rapamycin. We modeled these combinatorial therapies on various melanoma cell lines in 3D and 2D systems and in vivo. Riluzole combined with mTOR inhibition is more effective at halting melanoma anchorage-independent growth and xenograft tumor progression than either agent alone. PI3K signaling changes associated with this combinatorial treatment shows that 3D (nanoculture) modeling of cell signaling more closely resembles in vivo signaling than monolayer models. Riluzole combined with mTOR inhibition is effective at halting tumor cell progression independent of BRAF mutational status. This makes this combinatorial therapy a potentially viable alternative for metastatic melanoma patients who are BRAF WT and are therefore ineligible for vemurafenib therapy.
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http://dx.doi.org/10.1016/j.tranon.2014.11.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350641PMC
February 2015

Investigation of halide-induced aggregation of Au nanoparticles into spongelike gold.

Langmuir 2014 Mar 3;30(10):2648-59. Epub 2014 Mar 3.

Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences , Suzhou 215163, PR China.

We present a facile method for fabricating spongelike Au structures by halide-induced aggregation and fusion of gold nanoparticles (AuNPs). Halide ions (F(-), Cl(-), Br(-), and I(-)) showed distinctly different effects on the synthesized AuNPs, which were characterized by localized surface plasmon resonance (LSPR) and dynamic light scattering measurements. A noticeable red-shift in the LSPR peak was found after Br(-) and I(-) ion treatment, which indicates the adsorption of halide atoms or ions on the AuNPs. The surface potential of AuNPs varied by treatment with different types of halides; this finding indicates that different halide ions have different effects on the AuNPs. Br(-) and I(-) ions showed strong affinity toward the AuNPs. The different affinities of halide ions toward the AuNPs play an important role in controlling the formation process of spongelike gold. Citrate ions adsorbed on AuNPs were displaced by halide ions to different extents. Such displacement determined the aggregation and fusion behaviors of the AuNPs and eventually the formation of different spongelike structures.
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http://dx.doi.org/10.1021/la4046447DOI Listing
March 2014

Activation of the glutamate receptor GRM1 enhances angiogenic signaling to drive melanoma progression.

Cancer Res 2014 May 3;74(9):2499-509. Epub 2014 Feb 3.

Authors' Affiliations: Division of Surgical Oncology, Department of Surgery; Division of Medical Oncology, Department of Medicine, Rutgers Robert Wood Johnson Medical School; Susan Lehman Cullman Laboratory for Cancer Research, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway; and Rutgers Cancer Institute of New Jersey, New Brunswick, New Jersey.

Glutamate-triggered signal transduction is thought to contribute widely to cancer pathogenesis. In melanoma, overexpression of the metabotropic glutamate receptor (GRM)-1 occurs frequently and its ectopic expression in melanocytes is sufficient for neoplastic transformation. Clinical evaluation of the GRM1 signaling inhibitor riluzole in patients with advanced melanoma has demonstrated tumor regressions that are associated with a suppression of the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) pathways. Together, these results prompted us to investigate the downstream consequences of GRM1 signaling and its disruption in more detail. We found that melanoma cells with enhanced GRM1 expression generated larger tumors in vivo marked by more abundant blood vessels. Media conditioned by these cells in vitro contained relatively higher concentrations of interleukin-8 and VEGF due to GRM1-mediated activation of the AKT-mTOR-HIF1 pathway. In clinical specimens from patients receiving riluzole, we confirmed an inhibition of MAPK and PI3K/AKT activation in posttreatment as compared with pretreatment tumor specimens, which exhibited a decreased density of blood vessels. Together, our results demonstrate that GRM1 activation triggers proangiogenic signaling in melanoma, offering a mechanistic rationale to design treatment strategies for the most suitable combinatorial use of GRM1 inhibitors in patients.
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http://dx.doi.org/10.1158/0008-5472.CAN-13-1531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4008638PMC
May 2014

Fabrication and characterization of single electron tunneling device on Au/SiO2 nanocomposite films.

J Nanosci Nanotechnol 2013 Feb;13(2):878-82

School of Science, National University of Defense Technology, Changsha 410073, China.

Amino-functionalized mesoporous silica thin films (AF-MSTFs) have been directly dip-coated on silica wafers by co-condensation of tetraethoxysilane (TEOS) and 3-aminopropyltriethoxysilane (APTES) in the presence of Brij56 (C16H33(OCH2CH2)10OH) under acidic condition. Using the AF-MSTFs as templates, gold nanoparticles (GNPs) are well formed within the mesopores. The transmission electron microscope images and X-ray diffraction patterns demonstrate that the MSTFs are consistent with well ordered mesostructures and the GNPs are well crystallized in the templates. A single electron tunneling device based on self-assembled GNPs of the Au/SiO2 nanocomposite film has been fabricated by using nanolithographic definitions. The device shows Coulomb stair-cases at 77 K. The data are interpreted as single electron tunneling through the GNP which served as a Coulomb island between the source and drain electrodes. The charging energy is estimated to be much larger than the thermal energy at 77 K. The Coulomb island size calculated from the experimental data is -3.8 nm, which is consistent with the size of the GNPs self-assembled in the MSTFs.
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http://dx.doi.org/10.1166/jnn.2013.6070DOI Listing
February 2013

Identification of somatic mutations in human prostate cancer by RNA-Seq.

Gene 2013 May 19;519(2):343-7. Epub 2013 Feb 19.

Department of Urinary Surgery, Fengxian Branch of Shanghai Sixth People's Hospital, Shanghai, 201499, China.

RNA-Seq is a recently developed tool to characterize transcriptomes using a massively parallel sequencing technique. In spite of its broad usage in gene expression profiling, RNA-Seq can also be used to discover single nucleotide variants in transcribed regions. Here we report the result of an analysis of transcriptome sequencing data of 5 human prostate cancer tissues. A total of 116 disruptive mutations (frameshift indels or nonsynonymous nucleotide substitutions) in 92 genes are revealed with high confidence. Among these genes, several candidates are of particular interest. For example, a frameshift insertion/deletion (indel) is found in the coding region of TNFSF10, which disrupts the intact open reading frame and undermines the ability of TNFSF10 to induce apoptosis, in consequence promoting abnormal tumor progression. In summary, our findings demonstrate the use of RNA-Seq in somatic mutation screening, and provide a list of candidate genes which can be used in prostate cancer diagnosis and treatment.
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http://dx.doi.org/10.1016/j.gene.2013.01.046DOI Listing
May 2013

Macrophages play a key role in early blood brain barrier reformation after hypothermic brain injury.

Neurosci Lett 2011 Sep 18;501(3):148-51. Epub 2011 Jul 18.

Department of Pediatrics, The Cancer Institute of New Jersey, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, USA.

The inflammatory response following traumatic injury to the central nervous system (CNS) includes the infiltration of large numbers of macrophages. This response has been implicated in both ongoing tissue damage as well as recovery following CNS injury. We investigated the role of invading macrophages on one important aspect of tissue repair in the brain, the reformation of the blood brain barrier (BBB). We used liposomal clodronate to deplete monocytes and tissue macrophages. This method led to a marked reduction in the accumulation of F4/80-expressing cells at sites of hypothermic brain injury in a murine model. The integrity of the blood brain barrier over time following injury was assessed by permeability of fluorescent labeled albumin. The reduction in macrophages at the injury site was accompanied by a delay in early reformation of the blood brain barrier. In control animals the permeability of the BBB to FITC-labeled albumin returned to normal levels by seven days post-injury. In macrophage-depleted mice leakage of albumin was still observed at seven days post-injury. These results suggest that macrophages play an important role in early post-traumatic reformation of the BBB.
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http://dx.doi.org/10.1016/j.neulet.2011.06.062DOI Listing
September 2011

Age-dependent epigenetic control of differentiation inhibitors is critical for remyelination efficiency.

Nat Neurosci 2008 Sep;11(9):1024-34

Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854, USA.

The efficiency of remyelination decreases with age, but the molecular mechanisms responsible for this decline remain only partially understood. In this study, we show that remyelination is regulated by age-dependent epigenetic control of gene expression. In demyelinated young brains, new myelin synthesis is preceded by downregulation of oligodendrocyte differentiation inhibitors and neural stem cell markers, and this is associated with recruitment of histone deacetylases (HDACs) to promoter regions. In demyelinated old brains, HDAC recruitment is inefficient, and this allows the accumulation of transcriptional inhibitors and prevents the subsequent surge in myelin gene expression. Defective remyelination can be recapitulated in vivo in mice receiving systemic administration of pharmacological HDAC inhibitors during cuprizone treatment and is consistent with in vitro results showing defective differentiation of oligodendrocyte progenitors after silencing specific HDAC isoforms. Thus, we suggest that inefficient epigenetic modulation of the oligodendrocyte differentiation program contributes to the age-dependent decline in remyelination efficiency.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2656679PMC
http://dx.doi.org/10.1038/nn.2172DOI Listing
September 2008
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