Publications by authors named "Jiacheng Xu"

18 Publications

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Author Correction: Identification and characterization of Piwi-interacting RNAs in human placentas of preeclampsia.

Sci Rep 2021 Sep 14;11(1):18652. Epub 2021 Sep 14.

Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

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http://dx.doi.org/10.1038/s41598-021-98274-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440604PMC
September 2021

Identification and characterization of Piwi-interacting RNAs in human placentas of preeclampsia.

Sci Rep 2021 08 3;11(1):15766. Epub 2021 Aug 3.

Department of Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Preeclampsia is a common disease of pregnancy that poses a serious threat to the safety of pregnant women and the fetus; however, the etiology of preeclampsia is inconclusive. Piwi-interacting RNAs (piRNAs) are novel non-coding RNAs that are present at high levels in germ cells and are associated with spermatogenesis. Emerging evidence demonstrated that piRNA is expressed in a variety of human tissues and is closely associated with tumorigenesis. However, changes in the piRNA expression profile in the placenta have not been investigated. In this study, we used small RNA sequencing to evaluate the differences in piRNA expression profiles between preeclampsia and control patients and potential functions. Differential expression analysis found 41 up-regulated and 36 down-regulated piRNAs in preeclamptic samples. In addition, the functional enrichment analysis of piRNAs target genes indicated that they were related to the extracellular matrix (ECM) formation and tissue-specific. Finally, we examined the expression pattern of the PIWL family proteins in the placenta, and PIWL3 and PIWIL4 were the primary subtypes in the human placenta. In summary, this study first summarized the changes in the expression pattern of piRNA in preeclampsia and provided new clues for the regulatory role of piRNA in the human placenta.
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http://dx.doi.org/10.1038/s41598-021-95307-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333249PMC
August 2021

Adsorption desulfurization performance of PdO/[email protected] oxide hybrid aerogel: Influence of graphene oxide.

J Hazard Mater 2022 Jan 20;421:126680. Epub 2021 Jul 20.

Laboratory of Industrial Catalysis, College of Chemical Engineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou 310014, China.

Preparation of PdO/[email protected] oxide (GO) hybrid aerogels were carried out sol-gel method combined with atmospheric drying technology to study their adsorption performance for thiophenics and compared with PdO/SiO. Scanning electron microscope (SEM), N adsorption-desorption isotherms, X-ray diffraction (XRD) analysis, X-ray photoelectron spectroscopy (XPS), and fourier transformation infrared spectroscopy (FT-IR) for samples were performed. The adsorption performance of PdO/[email protected] for thiophene were better than that of PdO/SiO, attributed to that incorporation of GO increased the specific surface area and the Pd incorporation rate, where Pd ions acted as the π-complexation and sulfur-metal (SM) bond adsorption active centers, as well as GO adsorbed thiophene by the π-π stacking effect. The adsorption capacities of PdO/[email protected] for thiophene (TH), benzothiophene (BT) and dibenzothiophene (DBT) were 8.89, 9.3 and 12.6 mg-S/g, respectively. The addition of GO in aerogels could improve the inhibition effect of toluene, cyclohexene and pyridine while decreased the inhibition effect of MTBE and HO for the adsorption of thiophene, due to the π-π stacking effect and the hydrophobicity of GO, respectively. The adsorption process was spontaneous and exothermic, be well fitted by the apparent second-order kinetic model and dominated by chemical interaction. Pd/[email protected] had a good solvent elution regeneration performance.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126680DOI Listing
January 2022

LOXL1 exerts oncogenesis and stimulates angiogenesis through the LOXL1-FBLN5/αvβ3 integrin/FAK-MAPK axis in ICC.

Mol Ther Nucleic Acids 2021 Mar 9;23:797-810. Epub 2021 Jan 9.

Department of Biliary-Pancreatic Surgery, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200120, China.

Aberrant expression of lysyl oxidase-like 1 (LOXL1) reportedly leads to fibrous diseases. Recent studies have revealed its role in cancers. In this study, we observed an elevated level of LOXL1 in the tissues and sera of patients with intrahepatic cholangiocarcinoma (ICC) compared with levels in nontumor tissues and sera of unaffected individuals. Overexpression of LOXL1 in RBE and 9810 cell lines promoted cell proliferation, colony formation, and metastasis and and induced angiogenesis. In contrast, depletion of LOXL1 showed the opposite effects. We further showed that LOXL1 interacted with fibulin 5 (FBLN5), which regulates angiogenesis, through binding to the αvβ3 integrin in an arginine-glycine-aspartic (Arg-Gly-Asp) domain-dependent mechanism and enhanced the focal adhesion kinase (FAK)-mitogen-activated protein kinase (MAPK) signaling pathway inside vascular endothelial cells. Our findings shed light on the molecular mechanism underlying LOXL1 regulation of angiogenesis in ICC development and indicate that the LOXL1-FBLN5/αvβ3 integrin/FAK-MAPK axis might be the critical pathological link leading to angiogenesis in ICC.
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http://dx.doi.org/10.1016/j.omtn.2021.01.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868718PMC
March 2021

AGEs-RAGE axis causes endothelial-to-mesenchymal transition in early calcific aortic valve disease via TGF-β1 and BMPR2 signaling.

Exp Gerontol 2020 11 7;141:111088. Epub 2020 Sep 7.

Department of Cardiac Surgery, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China; Department of Clinical Medicine, Fujian Medical University, Fuzhou 350100, China. Electronic address:

Recent studies reported that advanced glycation end products (AGEs) and endothelial-to-mesenchymal transition (EndMT) were involved in the calcific aortic valve disease (CAVD). However, the roles of AGEs in EndMT in the development of CAVD have not been elucidated. In this study, six-week-old male Apoe mice were divided into four groups based on the following feeding periods: 0, 2, 4, and 6 months. The latter three groups were further divided into three subgroups corresponding to the following diet treatments: normal diet, high-fat diet + normal saline injection, and high-fat diet + aminoguanidine injection. Weight gain was monitored weekly. Finally, heart echocardiographic assessments were performed, and serum lipid levels, the protein expression and the histological changes in the aortic valves were determined. Results showed that the AGE inhibitor aminoguanidine alleviated the transaortic valve velocity and decreased the total cholesterol and low-density lipoprotein cholesterol levels. Calcification and carboxymethyl-lysine deposition were firstly detected around the aortic valve surfaces, whereas aminoguanidine injection attenuated their accumulation. In the early stage, HFD-activated AGEs-RAGE signaling resulted in the alpha-smooth muscle actin (α-SMA) upregulation and the vascular endothelium cadherin (VE-cadherin) downregulation on the valve endothelial layer. The activation resulted in early the transforming growth factor-β1 (TGF-β1) and the alkaline phosphatase (ALP) upregulation, and simultaneously reduced the bone morphogenetic protein receptor type II (BMPR2) expression. However, aminoguanidine restricted these proteins changes by inhibiting the interaction of AGEs and RAGE. In addition, immunofluorescence images showed obvious double-positive staining of ALP and α-SMA on the valve surfaces, revealing the contribution of EndMT to the early calcification. Therefore, this study demonstrates that activation of the AGEs-RAGE axis induced EndMT, promoting the progression of the aortic valve calcification in the initial stage via the counteraction of BMPR2 and TGF-β1 signaling.
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http://dx.doi.org/10.1016/j.exger.2020.111088DOI Listing
November 2020

Predicting the Outcome of Transcatheter Arterial Embolization Therapy for Unresectable Hepatocellular Carcinoma Based on Radiomics of Preoperative Multiparameter MRI.

J Magn Reson Imaging 2020 10 31;52(4):1083-1090. Epub 2020 Mar 31.

Department of Medical Imaging, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Beijing, China.

Background: In unresectable hepatocellular carcinoma (HCC), methods to predict patients at increased risk of progression are required.

Purpose: To investigate the feasibility of radiomics model in predicting early progression of unresectable HCC after transcatheter arterial chemoembolization (TACE) therapy using preoperative multiparametric magnetic resonance imaging (MP-MRI).

Study Type: Retrospective.

Population: A total of 84 patients with BCLC B stage HCC from one medical center. According to the modified response evaluation criteria in solid tumors, patients who progressed at 6 months after TACE therapy were assigned as the progressive disease (PD) group (n = 32). Patients whose MRI was performed on four devices were divided into a training cohort (n = 67). Patients whose MRI was performed on other than the previous four devices were used as the testing set (n = 17).

Field Strength/sequence: 3.0T, 1.5T axial T -weighted imaging (T WI), diffusion-weighted imaging (DWI, b = 0, 500 s/mm ), and apparent diffusion coefficient (ADC) ASSESSMENT: PD was confirmed via imaging studies with MRI. Risk factors, including age, alpha fetoprotein (AFP), size, and radiomic-related features of PD were assessed. In addition, the discrimination ability of each radiomics signature was tested on an independent testing set.

Statistical Tests: The area under the receiver-operator characteristic (ROC) curve (AUC) was used to evaluate the predictive accuracy of the radiomic signature in both the training and testing sets. The results indicated that the MP-MRI model achieved the greatest benefit.

Results: In the testing set, the model based on DWI features presented an AUC of (b = 0, 0.786; b = 500, 0.729), followed by T WI features (0.729) and ADC (0.714). The AUC of the MP-MRI signature was increased to 0.800 compared to any single MRI signature. The multivariate logistic analysis identified the radiomics signature as independent parameters of PD, while clinical information such as age, AFP, size, etc., had no significance in the PD group.

Data Conclusion: Preoperative MP-MRI has the potential to predict the outcome of TACE therapy for unresectable HCC. In addition, these image features may be complementary to the current staging systems of HCC patients.

Level Of Evidence: 2.

Technical Efficacy Stage: 3. J. Magn. Reson. Imaging 2020;52:1083-1090.
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http://dx.doi.org/10.1002/jmri.27143DOI Listing
October 2020

Detecting self-paced walking intention based on fNIRS technology for the development of BCI.

Med Biol Eng Comput 2020 May 21;58(5):933-941. Epub 2020 Feb 21.

Key Laboratory of Robotics and System of Jiangsu Province School of Mechanical and Electric Engineering, Soochow University, Suzhou, China.

Since more and more elderly people suffer from lower extremity movement problems, it is of great social significance to assist persons with motor dysfunction to walk independently again and reduce the burden on caregivers. The self-paced walking intention, which could increase the ability of self-control on the start and stop of motion, was studied by applying brain-computer interface (BCI) technology, a novel research field. The cerebral hemoglobin signal, which was obtained from 30 subjects by applying functional near-infrared spectroscopy (fNIRS) technology, was processed to detect self-paced walking intention in this paper. Teager-Kaiser energy was extracted at each sampling point for five sub-bands (0.0095~0.021 Hz, 0.021~0.052 Hz, 0.052~0.145 Hz, 0.145~0.6 Hz, and 0.6~2.0 Hz). Gradient boosting decision tree (GBDT) was then utilized to establish the detecting model in real-time. The proposed method had a good performance to detect the walking intention and passed the pseudo-online test with a true positive rate of 100% (80/80), a false positive rate of 2.91% (4822/165171), and a detection latency of 0.39 ± 1.06 s. GBDT method had an area under the curve value of 0.944 and was 0.125 (p < 0.001) higher than linear discriminant analysis (LDA). The results reflected that it is feasible to decode self-paced walking intention by applying fNIRS technology. This study lays a foundation for applying fNIRS-based BCI technology to control walking assistive devices practically. Graphical abstract Graphical representation of the detecting process for pseudo-online test. The lower figure is a partial enlargement of the upper figure. In the lower figure, the blue line represents the probability of walking predicted by GBDT without smoothing and the orange-red line represents the smoothed probability. The dark-red ellipse shows the effect of the smoothing-threshold method.
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http://dx.doi.org/10.1007/s11517-020-02140-wDOI Listing
May 2020

A Between-Subject fNIRS-BCI Study on Detecting Self-Regulated Intention During Walking.

IEEE Trans Neural Syst Rehabil Eng 2020 02 10;28(2):531-540. Epub 2020 Jan 10.

Objective: Most BCI (brain-computer interface) studies have focused on detecting motion intention from a resting state. However, the dynamic regulation of two motion states, which usually happens in real life, is rarely studied. Besides, popular within-subject methods also require an extensive and time-consuming learning stage when testing on a new subject. This paper proposed a method to discriminate dynamic gait- adjustment intention with strong adaptability for different subjects.

Methods: Cerebral hemoglobin signals obtained from 30 subjects were studied to decode gait-adjustment intention. Cerebral hemoglobin information was recorded by using fNIRS (functional near infrared spectroscopy) technology. Mathematical morphology filtering was applied to remove zero drift and EWM (Entropy Weight Method) was used to calculate the average hemoglobin values over Regions of Interest (ROIs). The gradient boosting decision tree (GBDT) was utilized to detect the onset of self-regulated intention. A 2-layer-GA-SVM (Genetic Algorithm-Support Vector Machine) model based on stacking algorithm was further proposed to identify the four types of self-regulated intention (speed increase, speed reduction, step increase, and step reduction).

Results: It was found that GBDT had a good performance to detect the onset intention with an average AUC (Area Under Curve) of 0.894. The 2-layer-GA-SVM model boosted the average ACC (accuracy) of four types of intention from 70.6% to 84.4% ( p = 0.005 ) from the single GA-SVM model. Furthermore, the proposed method passed pseudo-online test with the average results as following: AUC = 0.883, TPR (True Positive Rate) = 97.5%, FPR (False Positive Rate) = 0.11%, and LAY (Detection Latency) = -0.52 ± 2.57 seconds for the recognition of gait-adjustment intention; ACC = 80% for the recognition of adjusted gait.

Conclusion: The results indicate that it is feasible to decode dynamic gait-adjustment intentions from a motion state for different subjects based on fNIRS technology. It has a potential to realize the practical application of fNIRS-based brain-computer interface technology in controlling walking-assistive devices.
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http://dx.doi.org/10.1109/TNSRE.2020.2965628DOI Listing
February 2020

Teaching "Imaginary Objects" Symbolic Play to Young Children with Autism.

J Autism Dev Disord 2019 Oct;49(10):4109-4122

Chongqing Key Laboratory of Psychological Diagnosis and Education Technology for Children with Special Needs, School of Education, Chongqing Normal University, No. 37 Middle Route of University Town, Shapingba District, Chongqing, 401331, China.

Symbolic play skills are important in language acquisition and child development. Children with autism spectrum disorder (ASD) often have difficulties demonstrating such play behaviors. Imaginary objects symbolic play refers to play behavior in which children perform play actions without actual objects. Three boys with ASD (3-7 years) participated in this study. A multiple-probe across three participants and two settings design was employed to evaluate the effects of intraverbal training on the acquisition and generalization of imaginary objects symbolic play. Results indicated that all children acquired and maintained target imaginary objects play activities. Generalization to untaught activities occurred in one child. All three children' symbolic play emerged or increased in free play after the instruction.
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http://dx.doi.org/10.1007/s10803-019-04123-9DOI Listing
October 2019

Pilot Study on Gait Classification Using fNIRS Signals.

Comput Intell Neurosci 2018 17;2018:7403471. Epub 2018 Oct 17.

Key Laboratory of Robotics and System of Jiangsu Province, School of Mechanical and Electric Engineering, Soochow University, Suzhou, China.

Rehabilitation training is essential for motor dysfunction patients, and the training through their subjective motion intention, comparing to passive training, is more conducive to rehabilitation. This study proposes a method to identify motion intention of different walking states under the normal environment, by using the functional near-infrared spectroscopy (fNIRS) technology. Twenty-two healthy subjects were recruited to walk with three different gaits (including small-step with low-speed, small-step with midspeed, midstep with low-speed). The wavelet packet decomposition was used to find out the main characteristic channels in different motion states, and these channels with links in frequency and space were combined to define as feature vectors. According to different permutations and combinations of all feature vectors, a library for support vector machines (libSVM) was used to achieve the best recognition model. Finally, the accuracy rate of these three walking states was 78.79%. This study implemented the classification of different states' motion intention by using the fNIRS technology. It laid a foundation to apply the classified motion intention of different states timely, to help severe motor dysfunction patients control a walking-assistive device for rehabilitation training, so as to help them restore independent walking abilities and reduce the economic burdens on society.
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http://dx.doi.org/10.1155/2018/7403471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207899PMC
December 2018

Inhibition of C-X-C motif chemokine 10 reduces graft loss mediated by memory CD8 T cells in a rat cardiac re-transplant model.

Exp Ther Med 2018 Feb 1;15(2):1560-1567. Epub 2017 Dec 1.

Organ Transplantation Institute, Medical College, Xiamen University, Xiamen, Fujian 361005, P.R. China.

The interaction of chemokine (C-X-C motif) ligand 10 (CXCL10) with its receptor (CXCR3) is a critical process in recruiting donor reactive T cells to a graft and alloantigen-specific memory T (Tm) cells exert a principal function in promoting graft dysfunction during accelerated cardiac rejection. However, whether CXCL10 chemokine exerts any effects on acute accelerated rejection mediated by CD8 Tm cells in a re-transplant model has remained elusive. The present study established a cardiac transplant model by advanced microsurgery technology and improved organ storage. A novel rat model of cardiac re-transplantation was established at 40 days following primary heart transplant. The experiment included two parts, and when models were established, the rats were divided into two groups: Primary cardiac transplant (HTx) and re-transplantation without treatment (HRTx). In part 1, recipients from part 2, including re-transplantation without treatment (HRTx+NS) and re-transplantation treated with anti-CXCL10 antibodies (500 µg every other day by intraperitoneal injection; HRTx+CXCL10 Abs group). The graft survival time was observed and graft infiltration by inflammatory cells was assessed via histology of cardiac graft sections; in addition, the gene expression and the serum concentration of CXCL10 in each group was assessed. Indexes such as rejection-associated cytokines were assayed by reverse-transcription quantitative PCR and ELISA kits, and flow cytometry of splenocytes was used to detect Tm cells in the re-transplantation groups. The results demonstrated that level of CXCL10 was significantly increased and the graft mean survival time was shortened accompanied with aggravated lymphocyte cell infiltration in the HRTx group when compared that in the HTx group; in addition, the serum levels and mRNA expression of interleukin (IL)-2 and interferon (IFN)-γ were increased, while transforming growth factor (TGF)-β was decreased in the HRTx group. Furthermore, neutralization of CXCL10 prolonged the graft mean survival time and delayed accelerated rejection. Compared with that in the HRTx+NS group, serum levels and graft tissue mRNA expression of IFN-γ and IL-2 were decreased in the HRTx+CXCL10 Abs group, while TGF-β mRNA was significantly increased but the serum concentration was not significantly affected. In addition, there was no difference in IL-10 between the two groups, while delayed accelerated rejection paralleled with inflammatory cell infiltration decreased and the proliferation and differentiation of CD8 Tm cells in secondary lymphoid organs were reduced in the HRTx+CXCL10 Abs group vs. those in the HRTx+NS group. The present study demonstrated that CXCL10 had a crucial role in cardiac transplantation and re-transplantation, and that treatment with CXCL10 antibodies delays accelerated acute rejection mediated by Tm cells in a rat model of cardiac re-transplantation.
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http://dx.doi.org/10.3892/etm.2017.5585DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776636PMC
February 2018

The assessment of femoral shaft morphology in the sagittal plane in Chinese patients with osteoarthritis-a radiographic analysis.

J Orthop Surg Res 2017 Aug 30;12(1):127. Epub 2017 Aug 30.

Department of Sports Medicine and Adult Reconstructive Surgery, Drum Tower Hospital, School of Medicine, Nanjing University, 321 Zhongshan Road, Nanjing, Jiangsu, 210008, China.

Background: The purpose of this study was to analyze femoral shaft sagittal parameters in Chinese osteoarthritis (OA) patients undergoing total knee arthroplasty (TKA) and identify whether the parameters in the coronal plane could be predictors of those in the sagittal plane.

Methods: Standard long-standing anteroposterior and femoral lateral radiographs of 50 lower limbs in 50 Chinese OA patients were included. Sagittal femoral bowing angle (sFBA), angle between femoral distal anterior cortex axis and sagittal mechanical axis (DACSMA), angle between femoral distal anterior cortex axis and sagittal distal anatomic axis (DACSDAA), and angle between femoral sagittal mechanical axis and sagittal distal anatomic axis (SMADAA) were measured. Then the relationship between femoral shaft parameters in the sagittal and coronal planes were identified, including coronal femoral bowing angle (cFBA), valgus angle, hip-knee-ankle angle (HKA), length of femur (LF), femoral offset, femoral neck stem angle (FNS), and mechanical lateral distal femoral angle (mLDFA). A two-sided Pearson correlation coefficient was obtained to identify the correlations between parameters in the coronal and sagittal planes. P values <0.05 were considered statistically significant.

Results: The mean sFBA was 15.08° ± 3.79°, the mean DACSMA was 1.35° ± 2.70°, the mean DACSDAA was -2.66° ± 2.05°, and the mean SMADAA was 4.01° ± 2.55°. No correlation between parameters in the coronal and sagittal planes was found.

Conclusions: In this study, the discreteness of DACSMA, DACSDAA, and SMADAA in Chinese OA patients is high and this may affect the position of femoral prosthesis after TKA using the conventional intramedullary device. No parameters in the coronal plane are found correlated with those in the sagittal plane.

Trial Registration: Researchregistry2337.
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http://dx.doi.org/10.1186/s13018-017-0626-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577760PMC
August 2017

Can patients with femoral neck fracture benefit from preoperative thromboprophylaxis?: A prospective randomized controlled trial.

Medicine (Baltimore) 2017 Jul;96(29):e7604

Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University Laboratory for Bone and Joint Diseases, Model Animal Research Center, Nanjing University Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital Affiliated with the Medical School of Nanjing University, Nanjing, Jiangsu Department of Orthopedics, The first Affiliated Hospital of Nantong University, Nantong NingBo No.6 Hospital, Ningbo, P.R. China.

Background: The effectiveness of preoperative thromboprophylaxis remains obscure in patients with femoral neck fracture. The purpose of the current study was to investigate whether these patients benefit from preoperative thromboprophylaxis.

Methods: In this prospective, randomized controlled trial, a total of 80 patients with femoral neck fracture were randomly assigned to receive either rivaroxaban or conservative treatment before surgery. For all patients, color Doppler ultrasound of both lower extremities was performed immediately after admission. The primary efficacy outcome was venous thromboembolism (VTE) defined as deep vein thrombosis (DVT) or pulmonary embolism (PE). The primary safety outcome was major bleeding.

Result: Compared with conservative treatment, rivaroxaban could significantly reduce the incidence of DVT from 19.5% (8/41) to 2.6% (1/39) (P = .016). Preoperatively, there were a total of 9 occurrences of DVT including 8 DVT in the conservative treatment group and 1 in the oral rivaroxaban group. All cases of DVT were asymptomatic, with 8 of them diagnosed as isolated muscular calf vein thromboses. There were no differences between the 2 groups in terms of the overall incidence of major bleeding.

Conclusion: Thromboprophylaxis with rivaroxaban prior to surgery can effectively reduce the risk of preoperative DVT for patients with femoral neck fracture without increasing the risk of bleeding.
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http://dx.doi.org/10.1097/MD.0000000000007604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5521947PMC
July 2017

Bioluminescence Sensing of γ-Glutamyltranspeptidase Activity In Vitro and In Vivo.

Anal Chem 2017 07 20;89(13):7017-7021. Epub 2017 Jun 20.

CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , Hefei, Anhui 230026, China.

γ-Glutamyltranspeptidase (GGT) is an important tumor biomarker but using a bioluminescence (BL) probe to real time monitor its activity has not been reported. Herein, we rationally designed two GGT-cleavable BL probes Glu-AmLH (1) and Glu-p-aminobenzyloxycarbonyl-AmLH (2), and successfully applied them for sensing GGT activity with high sensitivity and excellent selectivity both in vitro and in vivo. The results indicated that, although 2 had lower background BL signal than 1, GGT had higher catalytic efficiency for 1 than 2, and 1 was superior to 2 for sensing GGT activity in living cells and tumors. We envision that our probe 1 could be widely applied for the diagnosis of important GGT-related diseases in animal models in the near future.
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http://dx.doi.org/10.1021/acs.analchem.7b00567DOI Listing
July 2017

A High Capacity, Good Safety and Low Cost NaFeSiO-Based Cathode for Rechargeable Sodium-Ion Battery.

ACS Appl Mater Interfaces 2017 Jul 27;9(27):22369-22377. Epub 2017 Jun 27.

State Key Laboratory of Silicon Materials, Key Laboratory of Novel Materials for Information Technology of Zhejiang Province and School of Materials Science & Engineering, Zhejiang University , Hangzhou, Zhejiang 310027, P. R. China.

Rechargeable sodium-ion batteries (SIBs) are receiving intense interest because the resource abundance of sodium and its lithium-like chemistry make them low cost alternatives to the prevailing lithium-ion batteries in large-scale energy storage devices. Two typical classes of materials including transition metal oxides and polyanion compounds have been under intensive investigation as cathodes for SIBs; however, they are still limited to poor stability or low capacity of the state-of-art. Herein, we report a low cost carbon-coated NaFeSiO with simultaneous high capacity and good stability, owing to the highly pure Na-rich triclinic phase and the carbon-incorporated three-dimensional network morphology. The present carbon-coated NaFeSiO demonstrates the highest reversible capacity of 181.0 mAh g to date with multielectron redox reaction that occurred among various polyanion-based SIBs cathodes, which achieves a close-to-100% initial Coulombic efficiency and a stable cycling with 88% capacity retention up to 100 cycles. In addition, such an electrode shows excellent stability either charged at a high voltage of 4.5 V or heated up to 800 °C. The present work might open up the possibility for developing high capacity, good safety and low cost polyanion-based cathodes for rechargeable SIBs.
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http://dx.doi.org/10.1021/acsami.7b02385DOI Listing
July 2017

Alkaline Phosphatase-Triggered Simultaneous Hydrogelation and Chemiluminescence.

J Am Chem Soc 2017 01 11;139(3):1041-1044. Epub 2017 Jan 11.

CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.

Chemiluminescence (CL) has a higher signal-to-noise ratio than fluorescence, but the use of CL to track an enzyme-instructed self-assembly (EISA) process has not been reported. In this work, by coincubation of the hydrogelator precursor Fmoc-Phe-Phe-Tyr(HPO)-OH (1P) and the CL agent AMPPD (2) with alkaline phosphatase (ALP), we employed CL to directly characterize and image the simultaneous EISA process of 1P. Hydrogelation processes of 1P with and without 2 and the CL properties of 2 with and without 1P under ALP catalysis were systematically studied. The results indicated that 2 is an ideal CL indicator for ALP-triggered hydrogelation of 1P. Using an IVIS optical imaging system, we obtained time-course CL images of 2 to track the simultaneous hydrogelation process of 1P in the same solution. We envision that our CL method could be employed to track more biological EISA events in the near future.
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http://dx.doi.org/10.1021/jacs.6b11041DOI Listing
January 2017

Combination of C-X-C motif chemokine 9 and C-X-C motif chemokine 10 antibodies with FTY720 prolongs the survival of cardiac retransplantation allografts in a mouse model.

Exp Ther Med 2015 Mar 22;9(3):1006-1012. Epub 2015 Jan 22.

Organ Transplantation Institute, Medical College, Xiamen University, Xiamen, Fujian 361005, P.R. China.

The upregulation of chemokine genes and the subsequent T-lymphocyte recruitment to the graft are early events in the development of acute cardiac transplant rejection or cardiac allograft vasculopathy. In the present study, a combined immunosuppressive regimen of C-X-C motif chemokine 9 (CXCL9) antibody (Ab), CXCL10 Ab and FTY720 was used in order to reduce the infiltration of memory T lymphocytes and prolong graft survival in a retransplantation murine model. BALB/c donor hearts were transplanted heterotopically into C57BL/6 mice at day 28 after skin transplantation. The mice were divided into four groups: i) Control (normal saline), ii) CXCL9 Ab and CXCL10 Ab [150 μg; once daily (qd); intraperitoneal (ip)], iii) FTY720 (0.2 mg/day; qd; ip) and iv) combined (2 mg/kg/day; qd; ip). Measurements of the median survival time of the cardiac grafts, histological examination, reverse transcription-quantitative polymerase chain reaction analysis, enzyme-linked immunosorbent assay and a mixed lymphocyte reaction were performed. The median graft survival time of the combined group was prolonged (9.3 days) compared with that of the control group (3.5 days) (P<0.001). Histological examination revealed that the combined treatment group graft rejection pathological score was 0.50, while the control group score was 3.62 (P<0.001). In addition, the gene expression level of interleukin (IL)-2 was significantly lower and the levels of IL-10 and transforming growth factor-β (TGF-β) were significantly higher in the combined group compared with those in the control group (P<0.001). Furthermore, the serum concentration levels of IL-2 and interferon-γ (IFN-γ) were significantly lower (P<0.001) and the concentration of IL-10 was significantly higher (P<0.05) in the combined group compared with those in the control group. In the mixed lymphocyte reaction, T-cell proliferation was found to be significantly lower in the combined treatment group than that in the control group (P<0.001). In conclusion, treatment with CXCL9 Ab and CXCL10 Ab or FTY720 reduced the graft infiltration of inflammatory cells, inhibited T-cell proliferation and prolonged graft survival. The combined treatment regimen of CXCL9 Ab, CXCL10 Ab and FTY720 was found to significantly reduce the infiltration of inflammatory cells in the graft and prolong graft survival.
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http://dx.doi.org/10.3892/etm.2015.2204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4316950PMC
March 2015

Age of onset of blindness affects brain anatomical networks constructed using diffusion tensor tractography.

Cereb Cortex 2013 Mar 27;23(3):542-51. Epub 2012 Feb 27.

LIAMA Center for Computational Medicine, National Laboratory of Pattern Recognition, Institute of Automation, The Chinese Academy of Sciences, Beijing 100190, China.

Studying blindness with various onset ages may elucidate the ways that unimodal sensory deprivation at different periods of development shape the human brain. In order to determine the effect of the onset age on brain anatomical networks, we extended a previous study of 17 early blind (EB) subjects with an additional 97 subjects with various onset ages. We constructed binary anatomical networks of these subjects and sighted controls (SC) using diffusion tensor tractography and calculated the topological properties of the network. Based on onset age, the subjects were divided into congenitally blind (CB), EB, adolescent-blind (AB), and late-blind (LB) subgroups. The LB subjects demonstrated a greater connectivity density and a higher global efficiency, similar to the SC. The CB and EB subgroups showed large group differences from the other groups in their topological networks, specifically, a reduced connectivity density and a decreased global efficiency compared with the SC, especially in the frontal and occipital cortices. Additionally, significant correlations were found between age of onset and the topological properties of the anatomical network in the blind. Our results suggest that visual experience during an early period of development is critical for establishing an intact efficient anatomical network in the human brain.
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http://dx.doi.org/10.1093/cercor/bhs034DOI Listing
March 2013
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