Publications by authors named "Jia Wang"

2,499 Publications

  • Page 1 of 1

Correlation between Anxiety, Depression, and Sleep Quality in College Students.

Biomed Environ Sci 2022 Jul;35(7):648-651

School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan 430065, Hubei, China;Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan 430065, Hubei, China.

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http://dx.doi.org/10.3967/bes2022.084DOI Listing
July 2022

Combination of Hematoma Volume and Perihematoma Radiomics Analysis on Baseline CT Scan Predicts the Growth of Perihematomal Edema.

Clin Neuroradiol 2022 Aug 9. Epub 2022 Aug 9.

Department of Radiology, Northern Jiangsu People's Hospital, 225001, Yangzhou, China.

Purpose: The aim is to explore the potential value of CT-based radiomics in predicting perihematomal edema (PHE) volumes after acute intracerebral hemorrhage (ICH) from admission to 24 h.

Methods: A total of 231 patients newly diagnosed with acute ICH at two institutes were analyzed retrospectively. The patients were randomly divided into training (N = 117) and internal validation cohort (N = 45) from institute 1 with a ratio of 7:3. According to radiomics features extracted from baseline CT, the radiomics signatures were constructed. Multiple logistic regression analysis was used for clinical radiological factors and then the nomogram model was generated to predict the extent of PHE according to the optimal radiomics signature and the clinical radiological factors. The receiver operating characteristic (ROC) curve was used to evaluate the discrimination performance. The calibration curve and Hosmer-Lemeshow test were used to evaluate the consistency between the predicted and actual probability. The support vector regression (SVR) model was constructed to predict the overall value of follow-up PHE. The performance of the models was evaluated on the internal and independent validation cohorts.

Results: The perihematoma 5 mm radiomics signature (AUC: 0.875) showed good ability to discriminate the small relative PHE(rPHE) from large rPHE volumes, comparing to intrahematoma radiomics signature (AUC: 0.711) or perihematoma 10 mm radiomics signature (AUC: 0.692) on the training cohort. The AUC of the combined nomogram model was 0.922 for the training cohort, 0.945 and 0.902 for the internal and independent validation cohorts, respectively. The calibration curves and Hosmer-Lemeshow test of the nomogram model suggested that the predictive performance and actual outcome were in favorable agreement. The SVR model also predicted the overall value of follow-up rPHE (root mean squared error, 0.60 and 0.45; Pearson correlation coefficient, 0.73 and 0.68; P < 0.001).

Conclusion: Among patients with acute ICH, the established nomogram and SVR model with favorable performance can offer a noninvasive tool for the prediction of PHE after ICH.
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http://dx.doi.org/10.1007/s00062-022-01201-xDOI Listing
August 2022

Lipidomic analysis reveals the effect of passive smoking on facial skin surface lipid in females.

Chem Phys Lipids 2022 Aug 5:105228. Epub 2022 Aug 5.

Division of Public Health Service and Safety Assessment, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China; NMPA Key Laboratory for Monitoring and Evaluation of Cosmetics, Shanghai 200336, China. Electronic address:

Background: Smoking has toxic effects on the skin and can damage it. However, few studies have focused on the lipid profile changes of facial skin surface lipids (SSL) by passive smoking.

Method: A cross-sectional analytical study was conducted on middle-aged females volunteered from Henan, China to participate in the study. A total of 20 passive smoking females and 20 non-passive smoking females were recruited for this study. The components of skin surface lipids were measured by ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF-MS). Multivariate data analysis and enrichment analysis were used to investigate the differences in facial SSL between passive and non-passive smoking females.

Result: There were 1247 lipid entities identified in facial SSL between passive and non-passive smoking females. Significant differences in composition of facial SSL were observed between the two groups. After multivariate data analysis suggested, 28 significantly different lipids were identified and classified into four classes in SSL of the female cheeks. As well as 32 significantly different lipids were obtained in SSL of the female foreheads, which included three classes of lipids. Subsequent analysis revealed that the content of fatty acids (FA) in passive smoking females was significantly reduced and the content of glycerolipids (GL) and sphingolipids (SP) increased, compared with the control group.

Conclusion: These results indicated that an increase in GLs and SPs of facial lipids and a decrease in FAs in passive smoking females. These changes in lipids might be associated with oxidative stress and interference with signaling pathways by substances in smoke. And passive smoking affected facial SSL and changed the content and metabolism of skin lipids.
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http://dx.doi.org/10.1016/j.chemphyslip.2022.105228DOI Listing
August 2022

Integrated transcriptome and small RNA sequencing in revealing miRNA-mediated regulatory network of floral bud break in .

Front Plant Sci 2022 22;13:931454. Epub 2022 Jul 22.

Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, National Engineering Research Center for Floriculture, School of Landscape Architecture, Beijing Forestry University, Beijing, China.

MicroRNAs is one class of small non-coding RNAs that play important roles in plant growth and development. Though miRNAs and their target genes have been widely studied in many plant species, their functional roles in floral bud break and dormancy release in woody perennials is still unclear. In this study, we applied transcriptome and small RNA sequencing together to systematically explore the transcriptional and post-transcriptional regulation of floral bud break in . Through expression profiling, we identified a few candidate genes and miRNAs during different developmental stage transitions. In total, we characterized 1,553 DEGs associated with endodormancy release and 2,084 DEGs associated with bud flush. Additionally, we identified 48 known miRNAs and 53 novel miRNAs targeting genes enriched in biological processes such as floral organ morphogenesis and hormone signaling transudation. We further validated the regulatory relationship between differentially expressed miRNAs and their target genes combining computational prediction, degradome sequencing, and expression pattern analysis. Finally, we integrated weighted gene co-expression analysis and constructed miRNA-mRNA regulatory networks mediating floral bud flushing competency. In general, our study revealed the miRNA-mediated networks in modulating floral bud break in . The findings will contribute to the comprehensive understanding of miRNA-mediated regulatory mechanism governing floral bud break and dormancy cycling in wood perennials.
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http://dx.doi.org/10.3389/fpls.2022.931454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355595PMC
July 2022

Selection of internal reference gene for normalization of reverse transcription-quantitative polymerase chain reaction analysis in .

Front Vet Sci 2022 22;9:934907. Epub 2022 Jul 22.

College of Veterinary Medicine, Hunan Agricultural University, Changsha, China.

is the etiological agent of swine enzootic pneumonia (EP), which resulting in considerable economic losses in pig farming globally. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a major tool for gene expression studies. However, no internal reference genes for normalization of RT-qPCR data of have been reported. The aim of this study was to screen the most stable genes for RT-qPCR analysis in under different conditions. Therefore, a total of 13 candidate internal reference genes (, and ) of filtered according to the reported quantitative proteomic analysis and the internal reference gene frequently used in other bacteria were selected for RT-qPCR analysis. The mRNAs from different virulence strains (168, 168 L, J, NJ, and LH) at five different growth phases were extracted. The corresponding cycle threshold (Ct) values of the 25 reverse transcribed cDNAs using the 14 candidate genes were determined. Different internal reference genes or combinations were then screened for expression stability analysis using various statistical tools and algorithms, including geNorm, BestKeeper, and NormFinder software, to ensure the reliability of the analysis. Through further comprehensive evaluation of the RefFinder software, it is concluded that the gene was the most suitable internal reference gene for samples of the different virulence strains in different growth phases for , followed by , and .
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http://dx.doi.org/10.3389/fvets.2022.934907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355380PMC
July 2022

Flame Edge Detection Method Based on a Convolutional Neural Network.

ACS Omega 2022 Aug 22;7(30):26680-26686. Epub 2022 Jul 22.

Science and Technology on Electronic Test and Measurement Laboratory, North University of China, Taiyuan 030051, China.

In this study, an improved flame edge detector based on convolutional neural network (CNN) was proposed. The proposed method can generate edge graphs and extract edge graphs relatively effectively. Our network architecture was based on VGG16 primarily, the last two max-pooling operators and all full connection layers of the VGG16 network were deleted, and the rest was taken as the basic network. The images output by the five convolution layers were upsampled to the size of the input images and finally fused to the edge image. Error calculation and back propagation of the fusion image and label image are carried out to form a weakly supervised model. Using the open datasets BSDS500 to train the network, the ODS F-measure can reach 0.810. Various experiments were carried out on different flame and fire images, including butane-air flame, oxygen-ethanol flame, energetic material flame, and oxygen-acetylene premixed jet flame, and the infrared thermogram was also verified by our method. The results demonstrate the effectiveness and robustness of the proposed algorithm.
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http://dx.doi.org/10.1021/acsomega.2c02858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352261PMC
August 2022

Health Effects of Probiotics on Nonalcoholic Fatty Liver in the Life Cycle Based on Data Analysis.

Comput Math Methods Med 2022 29;2022:2123162. Epub 2022 Jul 29.

Department of Health Management, Aerospace Center Hospital, Beijing 10089, China.

Objective: To observe the effect of intestinal probiotics in the treatment of nonalcoholic fatty liver disease (NAFLD) and the effect on liver function and inflammatory factors.

Methods: 34 healthy rats were selected for the study and divided into 10 rats in the control group, 12 rats in the model group, and 12 rats in the treatment group according to the random number table method. The control group was given behavioral and lifestyle interventions, and the treatment group was given Bifidobacterium minus Black enteric capsules 5 g/(kg-d) by strong feeding method on the basis of the control group. The fatty liver index (FLI), liver ultrasound examination results, liver function, and inflammatory factor levels were compared among the three groups. After 8 weeks of treatment, there were statistically significant differences between the FLI values and liver ultrasound results of the three groups, and the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triacylglycerol (TG), and total cholesterol (TC) levels of the observation group were lower than those of the control group and the model group. The levels of serum high molecular weight lipocalin (HMW-APN) and interleukin 22 (IL-22) in the observation group were higher than those in the control group, and the levels of tumor necrosis factor- (TNF-) were lower than those in the control and model groups, and the differences were statistically significant ( < 0.05).

Conclusion: Intestinal probiotics can improve the clinical efficacy of patients with NAFLD, improve liver function, and alleviate the inflammatory response, in order to provide a theoretical basis for the clinical treatment of patients with NAFLD.
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http://dx.doi.org/10.1155/2022/2123162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355765PMC
August 2022

Clinical Value of TCCD for Evaluating the Prognosis of Patients with Severe Traumatic Brain Injury After Large Decompressive Craniectomy: A Retrospective Study.

Adv Ther 2022 Aug 7. Epub 2022 Aug 7.

Department of Ultrasound Diagnosis, Tangdu Hospital, Air Force Medical University, Xi'an, China.

Introduction: It is challenging to assess the prognosis of patients with severe traumatic brain injury (sTBI) after large decompressive craniectomy (DC). The aim of this study was to evaluate the clinical value of transcranial color-coded duplex sonography (TCCD) for assessing the prognosis of sTBI patients 6 months after large DC.

Methods: This was a retrospective observational study that consecutively enrolled 84 patients with sTBI who were followed up for prognosis until 6 months after large DC. The primary endpoint was the Glasgow Outcome Score (GOS). According to the GOS, patients were divided into an unfavorable prognosis group (GOS 1-3, n = 47) and a favorable prognosis group (GOS 4-5, n = 37).

Results: Significant between-group differences were found in age and hemodynamic parameters (systolic peak blood flow velocity, end-diastolic blood flow velocity, mean blood flow velocity, pulsatility index and resistance index) of the middle cerebral artery detected by TCCD (P < 0.05 for all). Subsequently, ridge regression was used to build a prognostic model for patients with large DC. Based on the cerebral hemodynamic parameters measured by TCCD and age, the mean (± standard deviation) area under the curve of the prognostic model in patients with sTBI after large DC was 0.76 ± 0.22. The sensitivity and specificity were 82.08% and 74.17%, respectively.

Conclusions: The cerebral hemodynamic parameters detected by TCCD, combined with age, may be used to predict the outcomes of patients with sTBI at 6 months after large DC. As a noninvasive method, TCCD has the potential to assess the prognosis of these patients.

Trial Registration: ChiCTR: ChiCTR1800019758. Registered 27 November 2018-retrospectively registered ( http://www.chictr.org.cn/index.aspx ).
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http://dx.doi.org/10.1007/s12325-022-02251-wDOI Listing
August 2022

The effect of arginine on inhibiting amyloid fibril derived from β-casein and the binding studies with multi-spectroscopic techniques.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jul 26;282:121681. Epub 2022 Jul 26.

Department of Natural Product Chemistry, Pharmacy College, Jilin University, Changchun, PR China. Electronic address:

In general, β-casein is a stable molecular chaperone. However, the fact that amyloid fibrils derived from β-casein has been reported in some cases, which were usually associated with some malignant breast diseases. As an important amino acid, arginine not only exhibits the significance in casein synthesis in mammary gland, but also has great potentiality in inhibiting the amyloid fibril formation. Therefore, the influence of arginine on the amyloid fibrils formed by β-casein and further molecular mechanism were studied firstly with multi-spectroscopic techniques in the present work. The results of Thioflavin T determination, particle size analysis, transmission electron microscope observation showed that arginine not only inhibited the aggregation of β-casein at the growth stage, but also depolymerized the mature amyloid fibrils at the saturation stage. The further fluorescence experiment results demonstrated that the complex was formed between β-casein and arginine. Besides, there was one binding site and 0.48 nm binding distance. The thermodynamic parameters like ΔG, ΔS, ΔH were all negative, showing their binding reaction was spontaneous, and hydrogen bond and van der Waals force were the possibly chief intermolecular forces. Furthermore, the synchronous fluorescence spectra showing that the conformation of β-casein was affected and its tyrosine residues were gradually buried inside the protein. Our research would provide new insights into the treatments for the breast amyloidosis.
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http://dx.doi.org/10.1016/j.saa.2022.121681DOI Listing
July 2022

CTPS1 suppresses proliferation and migration in colorectal cancer cells.

Cell Cycle 2022 Aug 1:1-12. Epub 2022 Aug 1.

Department of General Surgery, Hepatic-biliary-pancreatic Institute, Lanzhou University Second Hospital, Lanzhou, China.

Colorectal cancer (CRC) is now the third most prevalent tumor and one of the deadliest cancers worldwide, with an increasing prevalence every year. Therefore, we urgently need to understand the mechanisms regulating the progression of colorectal cancer and find potential diagnostic biomarkers. In this study, we performed an analysis using the TCGA and GEO databases to find a molecular biomarker for the diagnosis of CRC, namely CTPS1. The results of this analysis revealed that CTPS1 could promote tumor proliferation and metastasis. Furthermore, bioinformatics analysis revealed that CTPS1 promoted CRC progression through cell cycle and p53 pathways. Further investigation demonstrated that CTPS1 might be involved in the regulation of CCNB1, RRM2, GTSE1, CDK2 and CHEK2 genes. Moreover, PCR confirmed that CTPS1 regulated GTSE1 and CDK2 molecules. Then, western blot was used to verify that CTPS1 promoted the expression of GTSE1 and CDK2 by inhibiting the expression of p53. In summary, we identified an important diagnostic biomarker for CRC, namely CTPS1, and its importance was validated at the cellular level. These results suggest that CTPS1 could serve as a candidate biomarker for CRC and CTPS1 inhibitors may be a potential treatment for CRC.
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http://dx.doi.org/10.1080/15384101.2022.2105084DOI Listing
August 2022

LcERF19, an AP2/ERF transcription factor from , positively regulates geranial and neral biosynthesis.

Hortic Res 2022 22;9:uhac093. Epub 2022 Apr 22.

State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, China.

The APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factors (TFs) are involved in the regulation of specialized terpenoid biosynthesis. However, the AP2/ERF TFs in have not been characterized and their role in the biosynthesis of terpenoids is unknown. Here, 174 LcAP2/ERF TFs were identified in and categorized into four subfamilies: 27 AP2, 7 RAV, 1 Soloist, and 139 ERF. Transcriptomic and qRT-PCR assays both showed that the expression levels of were similar to that of terpene synthase in the pericarp, which is related to the synthesis of geranial and neral in . was further shown to encode a nuclear-localized protein and its expression was strongly induced by jasmonate. Yeast one-hybrid and dual-luciferase assays showed that LcERF19 associated with GCC box elements of the promoter and promoted its activity. Transient overexpression of in and overexpression of in tomato resulted in a significant increase in geranial and neral. Our findings show that LcERF19 enhances geranial and neral biosynthesis through activation of expression, which provides a strategy to improve the flavor of tomato and other fruits.
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http://dx.doi.org/10.1093/hr/uhac093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327096PMC
April 2022

[Plasma metabolomics in a deep vein thrombosis rat model based on ultra-high performance liquid chromatography-electrostatic field orbitrap high resolution mass spectrometry].

Se Pu 2022 Aug;40(8):736-745

School of Medicine, Shanxi Datong University, Datong 037009, China.

Deep vein thrombosis (DVT) is a venous thromboembolic disease characterized by high incidence, mortality, and sequelae. Therefore, the effective prevention of DVT has become a critical public health concern. However, due to its complexity, the pathophysiological mechanism of DVT remains unclear. Metabolomics can be employed to analyze disease characteristics and provide scientific evidence on the underlying mechanisms. In this study, an established left femoral vein ligation rat model of DVT (=10) was used and compared with sham surgery controls (=10). In the DVT group, rats were anesthetized using an intraperitoneal injection of 10% chloral hydrate (300 mg/kg), after which the hair was shaved and the groin disinfected. A 2-cm longitudinal incision was made along the midpoint of the left groin area, and then the left femoral vein was separated. The vein was partially ligated at its proximal end to shrink the blood vessel lumen to approximately half. Then, 0.4 mL of 10% hypertonic saline was slowly injected from the distal end of the left femoral vein. At the same time, the femoral vein turned dark red, which indicated the formation of thrombosis. Finally, the incision was sutured after verifying bleeding in the surrounding tissue. Keeping all other procedures the same as the DVT group, the vein in the control group was not ligated or stimulated using hyper-tonic saline. The abdominal aorta plasma from rats in each group was collected seven days later. Untargeted metabolomics analysis based on ultra-high performance liquid chromatography-electrostatic field orbitrap high resolution mass spectrometry (UHPLC-Orbitrap HRMS) was conducted to investigate the plasma metabolic profiles of the sham surgery control and DVT groups. Principal component analysis (PCA) and orthogonal to partial least squares discrimi-nant analysis (OPLS-DA) on metabolome data for multivariate statistical analysis were employed to assess differences in the metabolic profile between the two groups. The results revealed distinct profiles for the DVT and control groups. The selection criteria for the differential metabolites were the variable importance in the projection (VIP) values of OPLS-DA (VIP>1) and fold changes (FC) in the DVT group (FC≤0.5 or FC≥2, <0.05). The resulting 27 differential metabolites reflecting a metabolic disorder in the DVT group were selected and analyzed. Of these, the levels of 17 metabolites significantly increased in the DVT group, including trimethylamine -oxide (TMAO), 4-amino-2-methyl-1-naphthol, chenodeoxycholic acid, and 7-ketocholesterol, whereas the levels of 10 metabolites decreased, including 3-dehydroxycarnitine, phosphatidylcholine 22∶6/20∶2 (PC 22∶6/20∶2), diglyceride 18∶3/20∶4 (DG 18∶3/20∶4) and anserine. To identify the changes in the metabolic pathway reflected by these differential metabolites, a differential abundance (DA) analysis based on the Kyoto Encyclopedia of Genes and Genomes metabolic pathway was conducted. The results showed that the differences in the metabolic pathways between the DVT and control groups were mainly manifested in the primary bile acid biosynthesis, bile secretion, histidine metabolism, linoleic acid metabolism, glycerophospholipid metabolism, and -alanine metabolism pathways. Among them, the primary bile acid biosynthesis and bile secretion pathways were upregulated in the DVT group, whereas the glycerophospholipid metabolism, linoleic acid metabolism, and -alanine metabolism pathways were downregulated. The histidine metabolism pathway contained upregulated as well as downregulated metabolites, resulting in a DA score of 0. In conclusion, these results indicate that the plasma metabolic profiling of the DVT group was significantly altered, while the disordered metabolites and metabolic pathways could provide a reference to further understand the pathological mechanism of DVT and identify new drug targets.
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http://dx.doi.org/10.3724/SP.J.1123.2021.12024DOI Listing
August 2022

A Novel Vibration Piezoelectric Generator Based on Flexible Piezoelectric Film Composed of PZT and PI Layer.

Polymers (Basel) 2022 Jul 15;14(14). Epub 2022 Jul 15.

School of Mechanical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212100, China.

A novel piezoelectric generator based on soft piezoelectric film consisting of a polyimide (PI) sheet and lead zirconate titanate (PZT) is proposed to generate electric energy under the operating conditions of low-frequency and small-amplitude vibration. The theoretical model and working principle of the piezoelectric generator are discussed in detail. Using ANSYS software, a finite element analysis of the static and modal characteristics of the piezoelectric generator is carried out. Further, the output of the prepared piezoelectric generator is investigated by a home-made experimental platform. Results show that the transient excitation voltage of the generator increases with the increase in load resistance, and the continuous excitation voltage increases first and then remains almost stable. The maximum continuous power produced by the piezoelectric generator is about 4.82 mW. Furthermore, the continuous excitation voltage and power are in accordance with the simulation values when the load resistances are 20 kΩ and 25 kΩ, respectively.
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http://dx.doi.org/10.3390/polym14142871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319419PMC
July 2022

Association between urine cobalt and prevalence of kidney stones in Americans aged ≥ 20 years old.

Environ Sci Pollut Res Int 2022 Jul 26. Epub 2022 Jul 26.

Department of Urology, Institute of Urology, West China Hospital of Sichuan University, No. 37, Guoxue Alley, Chengdu, China.

To determine whether urine cobalt (Co) is associated with the prevalence of kidney stones, we conducted a cross-sectional study of participants (≥ 20 years) involved in the National Health and Nutrition Examination Survey (NHANES) between 2007 and 2018. The urine Co level was divided into four groups: 0.02-0.22, 0.22-0.36, 0.36-0.58, and 0.58-37.40 μg/L. The independent correlation between urine Co and prevalence of kidney stones was determined by logistic regression analyses. A total of 10,744 participants aged over 20 years that were not pregnant were eligible. Among them, 1041 participants reported ever having developed kidney stones. Patients with kidney stones developed significantly higher urine Co than the non-stone participants. The kidney stone patients were more likely to have been smoking ≥ 100 cigarettes in life; have hypertension, diabetes, and cancer; and engage in heavy activity. Multivariate logistic regression indicated a significantly positive relationship between the urine Co level and occurrence of kidney stones (OR 1.059, 95% CI 1.018-1.102, P = 0.00430). Moreover, the outcome remained unchanged after some sophisticated factors were adjusted (OR 1.059, 95% CI 1.001-1.120, P = 0.04635), and kidney stones were significantly related to a higher level of Co (OR (95% CI) = 0.22-0.36 μg/L: 1.111 (0.869, 1.421); 0.36-0.58 μg/L: 1.392 (1.095, 1.770); 0.58-37.40 μg/L: 1.712 (1.351, 2.170), and P for trend < 0.00001). So, urine Co concentration is positively associated with the prevalence of kidney stones. However, more high-quality prospective studies are needed to elucidate the causal correlation between Co level and kidney stones.
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http://dx.doi.org/10.1007/s11356-022-22200-yDOI Listing
July 2022

Production and structural characterization of the cytochrome P450 enzymes in carotene ring hydroxylation.

Methods Enzymol 2022 26;671:223-241. Epub 2022 Mar 26.

School of Life Sciences, Anhui University, Hefei, China. Electronic address:

The superfamily of cytochrome P450 (CYP) enzymes participate in diverse metabolic reactions. Although structural characterization of prokaryotic CYPs has been performed extensively, structural studies for eukaryotic, especially plant, CYPs remain scanty. Members of CYP family 97 are hydroxylases devoted to carotene ring hydroxylation. Specifically, members of the CYP97A and CYP97C subfamilies work synergistically to hydroxylate the β- and ɛ-rings of α-carotene to form lutein. The stereospecific reactions introduce an R-chiral center at the C-3 position of each cyclohexene ring. The Arabidopsis thaliana genome encodes CYP97A3, CYP97B3, and CYP97C1. Recently, we determined crystal structures of CYP97A3 in unliganded and retinaldehyde-bound forms and of CYP97C1 in complex with detergent octylthioglucoside. These structures showed how the active site favors pro-R-hydroxylation. This chapter summarizes the steps for production of CYP97A3, CYP97B3, and CYP97C1. The in vitro assays to test hydroxylase activity and ligand binding affinity are also described, including the steps for production of the redox partner. We also review the details for crystallization and structural determination of CYP97A3 and CYP97C1. Characterization of the active site by high-resolution structure analysis provides clues to substrate channeling, which awaits further investigation.
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http://dx.doi.org/10.1016/bs.mie.2022.03.002DOI Listing
July 2022

Secular Trends in the Burden of Multiple Myeloma From 1990 to 2019 and Its Projection Until 2044 in China.

Front Public Health 2022 8;10:938770. Epub 2022 Jul 8.

Clinical Research Service Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.

Objective: Multiple myeloma (MM) imposes a heavy burden in China. Understanding the secular trend of MM burden and projecting its future trend could facilitate appropriate public health planning and improve the management of MM.

Methods: Sex-specific incidence and mortality rates of MM in China from 1990 to 2019 were collected from the Global Burden of Disease 2019 study. The secular trend of MM burden was analyzed by joinpoint regression. Age-period-cohort model was used to analyze the effects of age, period, and birth cohort on MM burden and project future trends up to 2044.

Results: From 1990 to 2019, the age-standardized incidence and mortality rates of MM continued to increase in males. For females, the age-standardized rates were stable in MM incidence and decreased in MM mortality. Males had a higher disease burden of MM than females. Age effects were the most significant risk factor for MM incidence and mortality. Moreover, the risk of MM incidence and mortality increased with increasing time period but decreased with birth cohort in males and females. The age-standardized incidence and mortality rates of MM in China is predicted to be continuously increasing over the next 25 years.

Conclusion: The burden of MM in China is expected to continue to increase in the future, with significant sex difference. A comprehensive understanding of the risk characteristics and disease pattern of MM could help develop timely intervention measures to effectively reduce its burden.
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http://dx.doi.org/10.3389/fpubh.2022.938770DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304978PMC
July 2022

Male Predisposition in Cerebellar Mutism Syndrome: a Cohort Study.

Cerebellum 2022 Jul 23. Epub 2022 Jul 23.

Department of Imaging Center, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, China.

The aim of this study was to explore the association between sex and cerebellar mutism syndrome and to examine other potential risk factors. This ambispective cohort study examined 218 pediatric patients (132 boys) with a posterior fossa tumor who underwent tumor resection from July 2013 to March 2021. The patients' demographics and tumor characteristics were examined and statistically analyzed to explore the associations among the variables. Multivariable and subgroup analyses were conducted to validate the independent risk factors for cerebellar mutism syndrome (CMS). The male and female patients did not differ significantly in terms of age, tumor size, tumor location, tumor consistency, VP shunt placement before resection, extent of resection, or surgeon, as well as with respect to the presence of hydrocephalus or paraventricular edema. The overall incidence of CMS was 32.6%. The incidence of CMS was significantly higher in male patients than that in female patients (41.7% vs. 18.6%; P = 0.001). In the multivariable analysis, male sex (adjusted odds ratio [OR], 3.27; P = 0.001), solid tumor consistency (adjusted OR, 5.61; P = 0.001), midline location (adjusted OR, 3.78; P = 0.004), and hydrocephalus (adjusted OR, 2.56; P = 0.047) were independent risk factors for the CMS. Chi-square analysis revealed that solid tumor consistency and midline location were associated with medulloblastoma (P < 0.001). Male patients had a higher risk of developing CMS after a posterior fossa tumor resection. Midline location, solid tumor consistency, and hydrocephalus were independent risk factors for CMS.
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http://dx.doi.org/10.1007/s12311-022-01449-6DOI Listing
July 2022

Long-term changes in insula-mesolimbic structural and functional connectivity in obese patients after laparoscopic sleeve gastrectomy.

Clin Auton Res 2022 Jul 21. Epub 2022 Jul 21.

Center for Brain Imaging, School of Life Science and Technology, Xidian University, Xi'an, 710071, Shaanxi, China.

Objective: Brain imaging studies have shown insula-related functional and structural abnormalities in patients with obesity. Laparoscopic sleeve gastrectomy is currently an effective procedure for treating obesity, which promotes acute recovery of brain functional and structural abnormalities in obese patients. The aim of this study was to investigate the long-term impact of laparoscopic sleeve gastrectomy on insula-related structural and functional connectivity.

Methods: Diffusion tensor imaging and resting-state functional magnetic resonance imaging were employed to investigate laparoscopic sleeve gastrectomy-induced changes in insula-related structural connectivity and corresponding resting-state functional connectivity in 25 obese patients prior to (PreLSG) and 12 months post-surgery (PostLSG).

Results: Results showed significant increases in fractional anisotropy and axial diffusivity between the right insula and anterior cingulate cortex, and higher fractional anisotropy of left insula-putamen, left insula-caudate and anterior cingulate cortex-right posterior cingulate cortex/precuneus at PostLSG12 compared with PreLSG. There were significant negative correlations between axial diffusivity of right insula-anterior cingulate cortex and body mass index, and fractional anisotropy of right insula-anterior cingulate cortex with scores on external eating at PostLSG12. Anxiety and depressive status ratings were negatively correlated with fractional anisotropy of left insula-putamen at PostLSG12. In addition, there was a significant decrease in resting-state functional connectivity between left insula and left caudate.

Conclusions: These findings demonstrate long-term changes in insula-related structural and functional connectivity abnormalities promoted by laparoscopic sleeve gastrectomy, which highlight its strong association with long-term weight loss and improvement in eating behaviors.
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http://dx.doi.org/10.1007/s10286-022-00877-yDOI Listing
July 2022

Understanding the nonradical activation of peroxymonosulfate by different crystallographic MnO: The pivotal role of Mn content on the surface.

J Hazard Mater 2022 Jul 16;439:129613. Epub 2022 Jul 16.

Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address:

Manganese oxide-activated persulfate plays a critical role in water purification and in situ chemical oxidation processes, but the underlying mechanism needs to be further revealed. Herein, the detailed mechanism of MnO with various crystallographic structures (α-, β-, γ-, and δ-MnO) towards peroxymonosulfate (PMS) activation was investigated. PMS activated by tunnel structured α-, β-, and γ-MnO showed higher acetaminophen (ACE) removal than layer structured δ-MnO with the removal efficiency following an order of α-MnO (85%) ≈ γ-MnO (84%) > β-MnO (65%) > δ-MnO (31%). Integrated with chemical quenching experiments, electron paramagnetic resonance, Raman spectra, X-ray photoelectron spectroscopy, and Langmuir-Hinshelwood model on kinetic data, both surface-bound PMS complexes and direct oxidation by surface manganese species (Mn) were disclosed as the dominant oxidation mechanism for ACE degradation in α-, β-, and γ-MnO/PMS, which were rarely observed in previous reports. Moreover, the catalytic activity of α-, β-, and γ-MnO was positively correlated to the Mn content on the catalyst surface. Higher content of Mn would stimulate the generation of more oxygen vacancies, which was conducive to the adsorption of PMS and the formation of reactive complexes. Overall, this study might provide deeper insight into the nonradical activation mechanism of PMS over different crystallographic MnO.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129613DOI Listing
July 2022

The Validity of Virtual Courage for Trainees in High-Risk Occupations.

Psychol Res Behav Manag 2022 14;15:1783-1796. Epub 2022 Jul 14.

Department of Developmental Psychology of Armyman, School of Psychology, Army Medical University, Chongqing, People's Republic of China.

Background: Employees in high-risk occupations are exposed to tremendous work stress that hinders organizational effectiveness and personal mental health. Based on positive psychology, courage can be considered a protective factor that buffers the adverse effect of high-risk surroundings on employees. However, little is known about the way courage is simulated or evaluated in response to safety concerns. Virtual reality (VR) is an accessible tool for courage simulation due to its immersive qualities, presence and interactive features and may provide a promising pathway to achieve a scientific, accurate and ecologically valid evaluation of high-risk employees.

Methods: The sample consisted of 51 high-risk employees who were recruited voluntarily. Before and after experiencing the VR courage scenarios, the participants completed the VR features questionnaire, the Physical Courage at Work Scale (PCWS), the Courage Measure (CM), and the Positive and Negative Affect Scale (PANAS). During the process of watching the VR courage scenarios, the participants' heart rate and skin conductance at resting-state baseline and during virtual courage scenarios were recorded through HTC VIVE Pro Eye and BioGraph Infiniti 8.

Results: The results support the hypothesis and reveal that the interaction, immersion and presence scores of the scenarios were all significantly higher than the median 4 points. The score for the CM in the posttest was significantly higher than that in the pretest. The scared and afraid scores for the posttest were significantly higher than those for the pretest. The heart rate and skin conductance of each scenario showed an increase compared with the baseline. The Pearson's correlation between physiological indicators and the score of the PCWS was 0.28~0.54.

Conclusion: This study developed virtual courage for high-risk occupations based on well-established theory and VR technology. Experimental data revealed that the paradigm conformed to the requirements of VR features and was able to activate fear and evoke the quality of courage. Thus, the virtual courage paradigms have good validity in simulating scenarios for high-risk employees, which might accelerate organizational effectiveness while buffering working stress.
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http://dx.doi.org/10.2147/PRBM.S371653DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9292066PMC
July 2022

In vivo study of dual functionalized mussel-derived bioactive peptides promoting 3D-printed porous Ti6Al4V scaffolds for repair of rabbit femoral defects.

J Biomater Appl 2022 Jul 20:8853282221117209. Epub 2022 Jul 20.

599923Changzhou Second People's Hospital of Nanjing Medical University, Changzhou, China.

The 3D printed porous titanium alloy scaffolds are beneficial to enhance angiogenesis, osteoblast adhesion, and promote osseointegration. However, titanium alloys are biologically inert, which makes the bond between the implant and bone tissue weak and prone to loosening. Inspired by the natural biological marine mussels, we designed four-claw-shaped mussel-derived bioactive peptides for the decoration of porous titanium alloy scaffolds: adhesion peptide-DOPA, anchoring peptide-RGD and osteogenic-inducing peptide-BMP-2. And the bifunctionalization of 3D-printed porous titanium alloy scaffolds was evaluated in vivo in a rabbit model of bone defect with excellent promotion of osseointegration and mechanical stability. Our results show that the in vivo osseointegration ability of the modified 3D printed porous titanium alloy test piece is significantly improved, and the bifunctional polypeptide coating group E has the strongest osseointegration ability. In conclusion, our experimental design partially solves the problems of stress shielding effect and biological inertness, and provides a convenient and feasible method for the clinical application of titanium alloy implants in biomedical implant materials.
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http://dx.doi.org/10.1177/08853282221117209DOI Listing
July 2022

Extracellular vesicles carrying proinflammatory factors may spread atherosclerosis to remote locations.

Cell Mol Life Sci 2022 Jul 18;79(8):430. Epub 2022 Jul 18.

Department of Neurology, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, Jiangsu, China.

Most cells involved in atherosclerosis release extracellular vesicles (EVs), which can carry bioactive substances to downstream tissues via circulation. We hypothesized that EVs derived from atherosclerotic plaques could promote atherogenesis in remote locations, a mechanism that mimics the blood metastasis of cancer. Ldlr gene knockout (Ldlr KO) rats were fed on a high cholesterol diet and underwent partial carotid ligation to induce local atherosclerosis. EVs were separated from carotid artery tissues and downstream blood of carotid ligation by centrifugation. MiRNA sequencing and qPCR were then performed to detect miRNA differences in EVs from rats with and without induced carotid atherosclerosis. Biochemical analyses demonstrated that EVs derived from atherosclerosis could increase the expression of ICAM-1, VCAM-1, and E-selectin in endothelial cells in vitro. EVs derived from atherosclerosis contained a higher level of miR-23a-3p than those derived from controls. MiR-23a-3p could promote endothelial inflammation by targeting Dusp5 and maintaining ERK1/2 phosphorylation in vitro. Inhibiting EV release could attenuate atherogenesis and reduce macrophage infiltration in vivo. Intravenously administrating atherosclerotic plaque-derived EVs could induce intimal inflammation, arterial wall thickening and lumen narrowing in the carotids of Ldlr KO rats, while simultaneous injection of miR-23a-3p antagomir could reverse this reaction. The results suggested that EVs may transfer atherosclerosis to remote locations by carrying proinflammatory factors, particularly miR-23a-3p.
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http://dx.doi.org/10.1007/s00018-022-04464-2DOI Listing
July 2022

Sports-related sudden cardiac death attributable to myocarditis: A systematic review and meta-analysis.

Can J Cardiol 2022 Jul 15. Epub 2022 Jul 15.

SportsCardiologyBC, University of British Columbia, Vancouver, British Columbia, Canada; Division of Cardiology, University of British Columbia, Vancouver, British Columbia, Canada. Electronic address:

Background: The incidence of sports-related sudden cardiac death (SrSCD) attributable to myocarditis is unknown. With the known association between SARS-CoV-2 (COVID-19) and myocarditis, an understanding of pre-pandemic rates of SrSCD due to myocarditis will be important in assessing a change of risk in the future. The objective was to ascertain the incidence of SrSCD or aborted sudden cardiac death (SCD) attributable to myocarditis in the general population.

Methods: A literature search through PubMed/Medline and Ovid/EMBASE was completed. Studies of SrSCD with autopsy data or clear cause aborted SrSCD were included. SrSCD was defined as SCD which occurred within 1 hour of exercise. Data were abstracted by two independent reviewers using the MOOSE guidelines. Risk assessment was performed using the Joanna Briggs Institute Critical Appraisal Checklist for Prevalence Studies. Random effects models were used to report the incidence and 95% confidence intervals (CIs). The primary outcome was the incidence of SrSCD attributable to myocarditis, and the secondary outcome was SrSCD overall.

Results: Fifteen studies were included comprising 347,092,437 person-years (PY). There were 1955 SrSCD or aborted SrSCD overall with an incidence of 0.93 (95% CI: 0.47 to 1.82) per 100,000 PY. Fifty-three SrSCD were attributed to myocarditis with an incidence of 0.047 (95% CI: 0.018 to 0.123) per 100,000 PY or 1 death attributable to myocarditis in 2.13 million PY.

Conclusions: In this meta-analysis, the overall incidence of SrSCD is low. Furthermore, SrSCD attributed to myocarditis is exceedingly rare.
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http://dx.doi.org/10.1016/j.cjca.2022.07.006DOI Listing
July 2022

Alterations in functional connectivity and interactions in resting-state networks in female patients with functional constipation.

Neurol Sci 2022 Jul 15. Epub 2022 Jul 15.

State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Air Force Medical University, Xi'an, 710032, Shaanxi, China.

BACKGROUND : Patients with functional constipation (FCon) have been reported with brain functional and structural abnormalities. However, no studies have been performed to investigate the differences in resting-state networks (RSNs) and changes in functional connectivity (FC) between RSNs in patients with FCon. Thus, the current study aimed to identify abnormal FC within and interaction between RSNs in patients with FCon to reveal the underlying neural mechanism.

Methods: Functional MRI with independent component analysis was applied to investigate alterations in FC within and functional network connectivity (FNC) between RSNs including default mode- (DMN), basal ganglia- (BGN), salience- (SN), and left and right control executive-networks (LCEN/RCEN) in 39 female patients with FCon and 36 female healthy controls (HC). Patient Assessment of Constipation Quality of Life Scale (PAC-QOL) and Patient Assessment of Constipation Symptom Scale (PAC-SYM) were used to assess the constipation symptoms.

Results: FCon patients had changed regional FC between different networks contributing to the abnormal FNC among RSNs compared with HC. Patients with greater stool syndromes had increased FNC of BGN-SN and DMN-LCEN, and patients with greater worries/concerns and PAC-QOL total score had reduced FNC of SN-RCEN. The greater strength changes in FC in prefrontal and parietal cortices were associated with higher negative emotion scores and greater rectal symptoms, respectively.

Conclusion: The findings suggested that FCon patients had altered FC within and interactions between RSNs and the brain FC changes were associated with constipation symptoms and altered emotions.
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http://dx.doi.org/10.1007/s10072-022-06275-6DOI Listing
July 2022

Janus kinase inhibitors in patients with ankylosing spondylitis: Great news, but some concerns on cardiovascular disease risk still exist. Author's reply.

Eur J Intern Med 2022 Jul 12. Epub 2022 Jul 12.

Department of Rheumatology, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China. Electronic address:

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http://dx.doi.org/10.1016/j.ejim.2022.07.004DOI Listing
July 2022

EIS biosensor based on a novel Myoviridae bacteriophage SEP37 for rapid and specific detection of Salmonella in food matrixes.

Food Res Int 2022 Aug 17;158:111479. Epub 2022 Jun 17.

Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China; College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

Recently, using bacteriophages as new molecular probes in reliable platforms for the detection of bacterial pathogens has attracted more and more increasing attentions. In this paper, a novel isolated Myoviridae bacteriophage SEP37 was covalently immobilized onto gold nanoparticles (AuNPs) modified gold disk electrode (GDE) surfaces using cysteamine (Cys) as a crosslinker. Substrates of GDE-AuNPs-Cys-Phage SEP37 and specific capture of Salmonella cells had been characterized using scanning electron microscopy (SEM) separately. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to study the electrochemical response of the biosensor interface manufacturing and bacterial capture process. Under the optimal experimental conditions, this phage-based EIS biosensor was able to detect Salmonella with a wide linear range from 2 × 10 to 2 × 10 colony forming unit (CFU)/mL within 30 min in spiked lake water and lettuce samples, with a limit of detection (LOD) of 17 CFU/mL. The detection linear range of spiked chicken samples was 2 × 10 to 2 × 10 CFU/mL, with a LOD of 1.3 × 10 CFU/mL. In combination with a pre-enrichment process for 3.5 h, this assay could reach a LOD of 1 CFU/mL in chicken breast meat samples. Besides, this phage-based EIS biosensor provided good reproducibility and stability. This phage-based EIS biosensor opens a new opportunity for the detection of pathogenic bacteria using the inherent selectivity of bacteriophage recognition.
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http://dx.doi.org/10.1016/j.foodres.2022.111479DOI Listing
August 2022

Application of Nano Drug Delivery System (NDDS) in Cancer Therapy: A Review.

Recent Pat Anticancer Drug Discov 2022 Jul 13. Epub 2022 Jul 13.

Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, P. R. China.

Background: Cancer is the biggest killer that threatens human health. Poor bioavailability and strong drug resistance of cancer drugs are common defects. In recent years, drug delivery therapy based on nanotechnology has become a focused research area, and nano drug delivery system has been widely studied in cancer treatment.

Objective: Based on the articles and patents published on the application of nano drug delivery systems in cancer treatment in the past five years, this paper summarizes the types of nano drug delivery systems and their advantages and limitations in cancer treatment, in order to provide reference for future anticancer research of nano drug delivery systems.

Methods: This perspective summarizes the types of nano drug delivery systems and their advantages and limitations in cancer treatment in recent five years, and proposes the development direction of nano drug delivery systems in the future.

Results: Based on the review of articles and patents, we found that the nano drug delivery system is mainly divided into encapsulated nano drug delivery system and covalently bound nano-prodrug delivery system. Its advantages in cancer treatment are mainly reflected in enhancing drug stability, improving bioavailability, reducing toxicity and better application in cancer diagnosis. However, nano drug delivery system is a new field of science, some have high toxicity and low bioavailability, the off-target phenomenon often occurs, and most studies are just focused in the early stage, its mechanism of action, clinical efficacy and patient tolerance, toxicity of treatment remains to be further investigated.

Conclusion: This perspective systematically summarizes the types of nano drug delivery systems and their advantages and limitations in cancer treatment based on the published articles and patents obtained in the last five years. Future research on nano drug delivery system should consider its potential risks, and design stable and efficient nano drug delivery system to treat cancer by changing nanomaterials or functionalizing nanomaterials.
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http://dx.doi.org/10.2174/1574892817666220713150521DOI Listing
July 2022

Effect of Acute Cold Exposure on Energy Metabolism and Activity of Brown Adipose Tissue in Humans: A Systematic Review and Meta-Analysis.

Front Physiol 2022 28;13:917084. Epub 2022 Jun 28.

Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China.

The benefit of cold exposure for humans against obesity has brought the energy metabolism and activity of brown adipose tissue (BAT) induced by cold into focus. But the results are inconsistent. This review is aimed to systematically explore the effect of cold exposure on the activity of BAT and energy metabolism in humans. We searched relevant papers that were published from 1990 to 2021 and were cited in PubMed Central, Web of science, Embase and Cochrane Library databases to conduct this systematic review and meta-analysis. Energy metabolism, BAT volume, BAT activity and non-esterified fatty acids (NEFA) data reported in eligible researches were extracted. Meta-analysis was applied to combine the mean difference or standard mean difference with their 95% confidence intervals (95%CI). RevMan 5.3 software was used for meta-analysis and evaluating the risk of bias. Stata 16.0 was used for evaluating the publication bias. Ten randomized controlled trials were included in meta-analysis. Compared with human exposed in room temperature at 24°C, the energy expenditure (EE) was increased after acute cold exposure at 16∼19°C (Z = 7.58, < 0.05, mean different = 188.43kal/d, 95% CI = 139.73-237.13); BAT volume (Z = 2.62, < 0.05; standard mean different = 0.41, 95% CI = 0.10-0.73); BAT activity (Z = 2.05, = 0.04, standard mean difference = 1.61, 95% CI = 0.07-3.14) and the intake of BAT NEFA (Z = 2.85, < 0.05; standard mean different = 0.53, 95% CI = 0.17-0.90) also increased. Acute cold exposure could improve the energy expenditure and BAT activity in adults, which is beneficial for human against obesity.
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http://dx.doi.org/10.3389/fphys.2022.917084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273773PMC
June 2022

High-Dimensional Immune Profiling by Mass Cytometry Revealed the Circulating Immune Cell Landscape in Patients With Intracranial Aneurysm.

Front Immunol 2022 27;13:922000. Epub 2022 Jun 27.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: Increasing evidence supports a critical role of chronic inflammation in intracranial aneurysm (IA). Understanding how the immunological alterations in IA provides opportunities for targeted treatment. However, there is a lack of comprehensive and detailed characterization of the changes in circulating immune cells in IA.

Objective: To perform a comprehensive and detailed characterization of the changes in circulating immune cells in patients with IA.

Methods: Peripheral blood mononuclear cell samples from IA patients (n = 26) and age-and sex-matched healthy controls (HCs, n = 20) were analyzed using high dimensional mass cytometry, and the frequency and phenotype of immune cell subtypes were assessed.

Results: We identified 28 cell clusters and found that the immune signature of IA consists of cluster changes. IA patients exhibited dysfunction of immunity, with dysregulation of CD4 T-cell clusters, increased B cells and monocytes, and decreased CD8 T cells, DNT cells, and DPT cells. Moreover, compared with findings in HC, IA was associated with enhanced lymphocyte and monocyte immune activation, with a higher expression of HLA-DR, CXCR3, and CX3CR1. In addition, the expression of TLR4, p-STAT3, and the exhaustion marker PD1 was increased in T cells, B cells, and NK cells in IA patients.

Conclusions: Our data provide an overview of the circulating immune cell landscape of IA patients, and reveal that the dysfunction of circulating immunity may play a potential role in the development of IA.
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http://dx.doi.org/10.3389/fimmu.2022.922000DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9271834PMC
June 2022

Potential ferroptosis-related diagnostic and prognostic biomarkers in laryngeal cancer.

Eur Arch Otorhinolaryngol 2022 Jul 12. Epub 2022 Jul 12.

Department of Otolaryngology, The First Hospital of China Medical University, Shenyang, 110001, Liaoning, China.

Purpose: Laryngeal cancer (LC) is a common malignant tumor of the head and neck. However, the relationship between ferroptosis and LC is still unclear. The aim of this study was to identify potential ferroptosis-related biomarkers for diagnosis and prognosis in LC.

Methods: We screened differentially expressed genes (DEGs) related to ferroptosis in LC from the TCGA and FerrDb database. DEGs were identified and enrichment by GO/KEGG, GSEA, GSVA analysis. PPI analysis was performed using String and Cytoscape, then hub genes were extracted. Furthermore, ROC analysis, pan-cancer analysis, gene mutation analysis, immune infiltration correlation analysis and clinical correlation analysis of hub genes were performed.

Results: A total of 59 DEGs were screened, which were more significantly enriched in biological processes and involved in HIF-1 signaling pathway, serotonergic synapse and ferroptosis. A total of 29 significant gene set pathways of LC data were performed by GSEA analysis. The GSVA analysis obtained 53 significant differential gene set pathways. The top 20 genes were identified by PPI. ROC curves revealed four of the top20 genes had a good performance, which were CA9 (AUC = 0.930), MAPK3 (AUC = 0.915), MUC1 (AUC = 0.945), and NOX4 (AUC = 0.933). Subsequent analysis found that CDKN2A has the highest mutation frequency in the top 20 gene, and IFNG had a significant correlation with age, tumor stage, degree of tumor differentiation and lymphatic clearance surgery.

Conclusion: Our study identified key genes closely related to ferroptosis in LC, which still need more studies to explore the mechanisms involved and may become effective clinical diagnostic and prognostic biomarkers.
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http://dx.doi.org/10.1007/s00405-022-07433-4DOI Listing
July 2022
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