Publications by authors named "Jia Jiang"

139 Publications

Hospital ethical climate associated with the professional quality of life among nurses during the early stage of COVID-19 pandemic in Wuhan, China: A cross-sectional study.

Int J Nurs Sci 2021 Jul 12;8(3):310-317. Epub 2021 May 12.

Xiangya School of Nursing, Central South University, Changsha, Hunan, China.

Objectives: To describe the professional quality of life and explore its associated factors among nurses coming from other areas of China to assist with the anti-epidemic fight in Wuhan and especially examine whether the hospital ethical climate was independently associated with nurses' professional quality of life.

Methods: A cross-sectional online survey was conducted from March 2020 to April 2020. The nurses working in Wuhan from the other parts of China were the target population. The Professional Quality of Life Scale version 5, the Hospital Ethical Climate Survey, and a basic information sheet were used to collect data. Descriptive statistics, -test, ANOVA, Pearson correlation, and multiple linear regression analysis were used to analyze the data.

Results: In total, 236 nurses participated in this study, and 219 valid questionnaires were analyzed. The average age of the participants was 31.2 ± 5.0 years. Most nurses were female (176/219; 80.4%) and married (145/219; 66.2%). In term of professional quality of life, nurses reported moderate (129/219; 58.9%) to high (90/219; 41.1%) levels of compassion satisfaction, low (119/219; 54.3%) to moderate (100/219; 45.7%) levels of burnout, and low (67/219; 36.0%) to high (10/219; 4.6%) levels of secondary traumatic stress. Regarding hospital ethical climate, nurses reported moderately high hospital ethical climates with an average score of 4.46. After controlling for socio-demographic characteristics, the multiple linear regression models showed that the hospital ethical climate subscale of "relationship with physicians" was independently associated with the compassion satisfaction ( = 0.533,  < 0.01) and burnout ( = -0.237,  < 0.05); the hospital ethical climate subscale of "relationship with peers" ( = -0.191,  < 0.01) was independently associated with the secondary traumatic stress.

Conclusions: During the early stage of the pandemic, nurses demonstrated moderate to high level of compassion satisfaction, low to moderate level of burnout, and all nurses experienced secondary traumatic stress. Nurses perceived a high level of hospital ethical climate, and the perceived hospital ethical climate played an important role in promoting nurses' professional quality of life during a life-threatening infectious disease pandemic.
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http://dx.doi.org/10.1016/j.ijnss.2021.05.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283712PMC
July 2021

Acceleration of ligamentization and osseointegration processes after anterior cruciate ligament reconstruction with autologous tissue-engineered polyethylene terephthalate graft.

Ann Transl Med 2021 May;9(9):770

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Background: Despite the advantages of excellent mechanical properties for rapid return to sports and early rehabilitation after anterior cruciate ligament (ACL) reconstruction with polyethylene terephthalate (PET) artificial ligament, the graft failure rate during long-term follow-up is relatively high due to poor graft-host incorporation. The purpose of the present study was to investigate the effect of autologous tissue-engineered PET (ATE-PET) grafts on osseointegration and ligamentization after ACL reconstruction.

Methods: Forty-eight New Zealand white rabbits were randomly divided into PET group (n=24) and ATE-PET group (n=24). In the ATE-PET group, the rabbits initially underwent subcutaneous implantation of the PET ligament. Two weeks later, unilateral ipsilateral ACL reconstruction was performed using an ATE-PET graft. In the PET group, the rabbits underwent ACL reconstruction using PET grafts as controls. Macroscopic observation, micro-computed tomography, histological and immunofluorescent staining, and biomechanical tests were conducted to evaluate the effects at 4 and 12 weeks postoperatively.

Results: The ATE-PET graft was highly pre-vascularized with myofibroblast aggregation after two weeks of subcutaneous implantation. With regard to the intraosseous part of the graft, the ATE-PET group had significantly higher bone mineral density and bone volume/total volume ratio at 12 weeks. Histologically, the width of the interface between the graft and bone was smaller. Regarding the intra-articular part, thicker tissue coverage with a glossy appearance was observed in the ATE-PET group at 12 weeks on macroscopic observation. Histological staining also showed more collagen fibers grew in the grafts with fewer inflammatory reactions of the ATE-PET group at both 4 and 12 weeks. Immunofluorescently, both α-SMA-positive vessels and α-SMA-positive myofibroblasts were found to be significantly greater around the graft in the ATE-PET group at 4 weeks and markedly declined at 12 weeks. Moreover, the ATE-PET group presented significantly greater failure load and stiffness than the PET group at 12 weeks (53.7±5.4 42.5±4.5 N, P<0.01; 12.9±3.0 9.8±1.3 N/mm, P=0.04).

Conclusions: The ATE-PET artificial ligament with pre-vascularization and myofibroblast aggregation could effectively accelerate intra-articular graft ligamentization and intraosseous graft osseointegration, thus enhancing the biomechanical properties after ACL reconstruction in a rabbit model.
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http://dx.doi.org/10.21037/atm-20-8048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246152PMC
May 2021

Effects of hydroxyethyl starch and gelatin on the risk of acute kidney injury following orthotopic liver transplantation: A multicenter retrospective comparative clinical study.

Open Med (Wars) 2021 23;16(1):322-331. Epub 2021 Feb 23.

Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Gongtinan Road, Chaoyang District, Beijing, 100020, China.

Objectives: This multicenter retrospective study aimed to compare the effects of HES and gelatin (GEL) on the risk of post-OLT AKI.

Method: A total of 1,672 patients undergoing OLT were enrolled from major transplant centers in China between 2005 and 2013. These patients were divided into three groups: GEL, hydroxyethyl starch (HES), and GEL + HES group.

Results: There was no significant difference in the incidence of post-OLT AKI among the GEL, HES, and GEL + HES groups. The GEL + HES group had a lower incidence of stage II post-OLT AKI than the other two groups. Compared with patients receiving GEL, patients receiving HES did not harbor an increased risk of AKI. Our results showed that MELD score (adjusted odds ratio [OR], 1.579; 95% confidence interval [CI], 1.123-2.219; = 0.009) and preoperative anemia (adjusted OR, 1.533; 95% CI, 1.212-1.939; 0.001) were independent risk factors for post-OLT AKI, and normal preoperative Scr level (vs abnormal; adjusted OR, 0.402; 95% CI, 0.222-0.729; = 0.003) was independent protective factors for post-OLT AKI.

Conclusion: This large-scale multicenter retrospective study found that the intraoperative use of HES did not increase the overall incidence of post-OLT AKI in patients when compared with GEL, and whether to increase the risk of post-OLT AKI needs to be further explored.
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http://dx.doi.org/10.1515/med-2020-0183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256391PMC
February 2021

A new, unusually dark, typhlocybine leafhopper (Hemiptera, Cicadellidae, Typhlocybinae, Erythroneurini) from China.

Zookeys 2021 4;1042:35-40. Epub 2021 Jun 4.

School of Karst Science, Guizhou Normal University/ State Engineering Technology Institute for Karst Desertification Control, Guiyang, Guizhou 550001, China Guizhou Normal University Guiyang China.

An unusually dark typhlocybine leafhopper (Cicadellidae, Typhlocybinae, Erythroneurini) from Guizhou Province, China, is described as a new genus and species, , and Detailed morphological descriptions and illustrations of the new species are provided.
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http://dx.doi.org/10.3897/zookeys.1042.63593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195945PMC
June 2021

Transcriptome analysis reveals Vernalization is independent of cold acclimation in Arabidopsis.

BMC Genomics 2021 Jun 21;22(1):462. Epub 2021 Jun 21.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

Background: Through vernalization, plants achieve flowering competence by sensing prolonged cold exposure (constant exposure approximately 2-5 °C). During this process, plants initiate defense responses to endure cold conditions. Here, we conducted transcriptome analysis of Arabidopsis plants subjected to prolonged cold exposure (6 weeks) to explore the physiological dynamics of vernalization and uncover the relationship between vernalization and cold stress.

Results: Time-lag initiation of the two pathways and weighted gene co-expression network analysis (WGCNA) revealed that vernalization is independent of cold acclimation. Moreover, WGCNA revealed three major networks involving ethylene and jasmonic acid response, cold acclimation, and chromatin modification in response to prolonged cold exposure. Finally, throughout vernalization, the cold stress response is regulated via an alternative splicing-mediated mechanism.

Conclusion: These findings illustrate a comprehensive picture of cold stress- and vernalization-mediated global changes in Arabidopsis.
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http://dx.doi.org/10.1186/s12864-021-07763-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218483PMC
June 2021

Design BH3 domain fusion protein as targeting pro-apoptotic self-assembling nanoparticles.

Biomed Pharmacother 2021 Jun 18;141:111825. Epub 2021 Jun 18.

Department of Biopharmaceutics, West China School of Pharmacy, Sichuan University, No. 17, Renmin Road, Chengdu 610041, Sichuan Province, PR China; Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy Sichuan University, Chengdu 610041 PR China. Electronic address:

Cancer is a serious global health issue, and apoptosis is a logical and practical cancer therapeutic strategy. Apoptosis responses to internal and external signals. Both BH3 domain in the pro-apoptotic proteins and truncated BH3 domain can stimulate cell apoptosis. However, the faults of peptides in systemic administration restrict the applications of truncated BH3 domain. Ferritin, as an attractive nanoparticle with the capacity of self-assemble to unique hollow spherical structure, could display truncated BH3 domain an N-terminal. Thus, in this study, we designed a pro-apoptosis self-assembling protein nanoparticle by BH3 domain fusion at N-terminal of ferritin. We evaluated the size, cytotoxicity and pro-apoptosis effect of these nanoparticles. The results showed that RGD-BH3-HFn, BH3-HFn and HFn had uniformly spherical structure with sizes at 26.08 ± 0.11 nm, 22.07 ± 0.67 nm, and 16.81 ± 0.88 nm, respectively; RGD-BH3-HFn has stronger cytotoxicity against tumor cells than BH3-HFn and HFn. The total apoptosis ratios (including necrosis) of C6 cells induced by RGD-BH3-HFn, BH3-HFn, and HFn proteins were 15.24%, 10.13% and 2.14%, respectively; those of bEnd.3 cells were 15.47%, 7.33% and 1.70%, respectively; while the total apoptosis rate (including necrosis) of MCF-7 cells were 3.24%, 4.9% and - 1.68%, respectively. The results suggested self-assembling RGD-BH3-HFn could target to C6 cells and bEnd.3 cells, and enhance tumor cells apoptosis, its apoptosis effect against C6 cells was 7.11-fold that of HFn, and apoptosis effect against bEnd.3 cells was 9.08-fold that of HFn. These results indicated BH3 domain can be designed as targeting pro-apoptotic nanoparticles.
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http://dx.doi.org/10.1016/j.biopha.2021.111825DOI Listing
June 2021

Arginine methylation of METTL14 promotes RNA N-methyladenosine modification and endoderm differentiation of mouse embryonic stem cells.

Nat Commun 2021 06 18;12(1):3780. Epub 2021 Jun 18.

Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, P.R. China.

RNA N-methyladenosine (mA), the most abundant internal modification of mRNAs, plays key roles in human development and health. Post-translational methylation of proteins is often critical for the dynamic regulation of enzymatic activity. However, the role of methylation of the core methyltransferase METTL3/METTL14 in mA regulation remains elusive. We find by mass spectrometry that METTL14 arginine 255 (R255) is methylated (R255me). Global mRNA mA levels are greatly decreased in METTL14 R255K mutant mouse embryonic stem cells (mESCs). We further find that R255me greatly enhances the interaction of METTL3/METTL14 with WTAP and promotes the binding of the complex to substrate RNA. We show that protein arginine N-methyltransferases 1 (PRMT1) interacts with and methylates METTL14 at R255, and consistent with this, loss of PRMT1 reduces mRNA mA modification globally. Lastly, we find that loss of R255me preferentially affects endoderm differentiation in mESCs. Collectively, our findings show that arginine methylation of METTL14 stabilizes the binding of the mA methyltransferase complex to its substrate RNA, thereby promoting global mA modification and mESC endoderm differentiation. This work highlights the crosstalk between protein methylation and RNA methylation in gene expression.
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http://dx.doi.org/10.1038/s41467-021-24035-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213825PMC
June 2021

Two new species of the genus Dworakowska from China (Hemiptera: Cicadellidae: Typhlocybinae).

Biodivers Data J 2021 3;9:e68043. Epub 2021 Jun 3.

School of Karst Science, Guizhou Normal University / State Key Laboratory Cultivation Base for Guizhou Karst Mountain Ecology Environment of China, Guiyang, 550001, China School of Karst Science, Guizhou Normal University / State Key Laboratory Cultivation Base for Guizhou Karst Mountain Ecology Environment of China Guiyang, 550001 China.

Background: The leafhopper genus Dworakowska, 1970 includes 33 species and is widely distributed in China, Korea, South Korea, Japan, Nepal, India, Thailand and Vietnam.

New Information: Two new species found at Bijie City and Shibing County, Guizhou Province, China are described and illustrated, and A key to distinguish the Chinese species of the genus is given.
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http://dx.doi.org/10.3897/BDJ.9.e68043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192415PMC
June 2021

[Essence of acupoints and meridians based on the studies of myofascial trigger points].

Zhongguo Zhen Jiu 2021 Jun;41(6):633-40

Department of Sports and Rehabilitation, Shanghai University of Sport, Shanghai 200438.

Based on the modern anatomy and physiology, the referred pain of myofascial trigger points of each muscle is integrated; compared with the twelve meridians as well as conception vessel and governor vessel, the similarity of their position and running course is observed. With the current research progress of myofascial trigger points and fasciology, based on the running course of referred pain of trigger points, combined with fascia mechanics, nerve and vascular, the location of acupoints and meridians, as well as the relationship between acupoints and meridians, are discussed.
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http://dx.doi.org/10.13703/j.0255-2930.20200327-k0001DOI Listing
June 2021

The Effect of Antiosteoporosis Therapy With Risedronate on Rotator Cuff Healing in an Osteoporotic Rat Model.

Am J Sports Med 2021 Jul 17;49(8):2074-2084. Epub 2021 May 17.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Background: Osteoporosis increases the revision rate of rotator cuff repair (RCR). Weak fixation might not be the only cause of high RCR failure rates. The biological mechanism associated with tendon-to-bone healing after RCR in osteoporosis should be investigated.

Hypothesis: (1) Osteoporosis would impair rotator cuff healing through the high osteoclastic activity at the repaired interface. (2) Risedronate would promote rotator cuff healing by reducing osteoclastic activity at the repaired interface.

Study Design: Controlled laboratory study.

Methods: A total of 84 female Sprague Dawley rats were randomly treated using ovariectomy or sham surgeries to establish osteoporotic and nonosteoporotic rat models. After confirming osteoporosis, a chronic rotator cuff tear model was created and RCR was performed. Postoperatively, osteoporotic rats were randomly divided into osteoporosis (OP) and osteoporosis with risedronate administration (OP+RIS) groups. Nonosteoporotic rats were used as the control (CON) group. Osteoclastic activity was measured at 1 and 3 weeks after RCR, and histologic analysis of the tendon-to-bone interface, bone morphometric evaluation, and biomechanical tests were performed at 4 and 8 weeks.

Results: At the early healing stages of 1 and 3 weeks after RCR, the OP group showed the highest osteoclast density at the repaired interface. Compared with the OP group, risedronate administration significantly decreased osteoclast density in the OP+RIS group. At 8 weeks, histologic scores were greater in the OP+RIS group than in the OP group but still lower than in the CON group. Histologic scores at 8 weeks were negatively correlated with osteoclast density at the early healing stage. Additionally, the OP+RIS group showed better bone morphometric parameters and biomechanical properties than did the OP group.

Conclusion: Osteoporosis impaired rotator cuff healing, which might be related to the high osteoclast density at the repaired interface at the early healing stage. Postoperative risedronate administration decreased osteoclast density and enhanced rotator cuff healing in osteoporotic rats, although the effect was inferior to that in nonosteoporotic rats.

Clinical Relevance: Postoperative risedronate administration can be considered a potential therapy to enhance rotator cuff healing in patients with postmenopausal osteoporosis. However, this needs to be verified in a clinical setting.
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http://dx.doi.org/10.1177/03635465211011748DOI Listing
July 2021

Mitogenome and phylogenetic analysis of typhlocybine leafhoppers (Hemiptera: Cicadellidae).

Sci Rep 2021 May 12;11(1):10053. Epub 2021 May 12.

School of Karst Science, State Key Laboratory Cultivation Base for Guizhou Karst Mountain Ecology Environment of China, Guizhou Normal University, Guiyang, 550001, Guizhou, China.

Mitogenomes have been widely used to estimate phylogenetic relationships among insects and provide data useful for augmenting traditional morphological characters in delimiting species. Here, complete mitogenome sequences of two closely related typhlocybine leafhoppers, Cassianeura cassiae (Ahmed, 1970) and C. bimaculata Dworakowska, 1984, were obtained and found to be 15,423 bp and 14,597 bp in length, respectively. The gene order was found to be similar to other published leafhopper mitogenomes, but the control region of C. bimaculata is the shortest among known leafhoppers and lacks tandem repeats. Phylogenetic analysis of 13 protein-coding genes (PCGs), the first and second codons of 13 PCGs, 13 PCGs and two rRNAs formed three well-supported tree topologies. The topologies of phylogenetic trees inferred from three datasets were almost identical, which was consistent with previous molecular phylogenies of this group. Comparative morphological study of the ovipositors revealed several characters potentially useful for diagnosing genera and resolving their phylogenetic relationships. Phylogenetic analysis of these and other morphological characters yielded a tree that is mostly consistent with the tree obtained from analysis of mitogenome sequences. In both molecular and morphological phylogenenies, Typhlocybini and Zyginellini clustered into one clade, but neither was recovered as monophyletic.
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http://dx.doi.org/10.1038/s41598-021-89525-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115509PMC
May 2021

Impact of intrauterine fetal resuscitation with oxygen on oxidative stress in the developing rat brain.

Sci Rep 2021 May 7;11(1):9798. Epub 2021 May 7.

Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO, 63110, USA.

Use of maternal oxygen for intrauterine resuscitation is contentious because of the lack of evidence for its efficacy and the possibility of fetal harm through oxidative stress. Because the developing brain is rich in lipids and low in antioxidants, it remains vulnerable to oxidative stress. Here, we tested this hypothesis in a term pregnant rat model with oxytocin-induced fetal distress followed by treatment with either room air or 100% oxygen for 6 h. Fetal brains from both sexes were subjected to assays for biomarkers of oxidative stress (4-hydroxynonenal, protein carbonyl, or 8-hydroxy-2'-deoxyguanosine), expression of genes mediating oxidative stress, and mitochondrial oxidative phosphorylation. Contrary to our hypothesis, maternal hyperoxia was not associated with increased biomarkers of oxidative stress in the fetal brain. However, there was significant upregulation of the expression of select genes mediating oxidative stress, of which some were male-specific. These observations, however, were not accompanied by changes in the expression of proteins from the mitochondrial electron transport chain. In summary, maternal hyperoxia in the setting of acute uteroplacental ischemia-hypoxia does not appear to cause oxidative damage to the developing brain.
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http://dx.doi.org/10.1038/s41598-021-89299-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105387PMC
May 2021

CT imaging of gold nanoparticle-labeled exosomes in a myocardial infarction mouse model.

Ann Transl Med 2021 Mar;9(6):504

Research Laboratory for Functional Nanomaterial, National Engineering Research Center for Nanotechnology, Shanghai, China.

Background: Acute myocardial infarction (MI) is the primary factor leading to cardiovascular diseases, which are the main causes of morbidity and mortality in developed countries. Mesenchymal stem cell (MSC)-derived exosomes have been reported to improve heart function after MI; however, the molecular mechanisms responsible for this are unknown. imaging can reveal the trafficking process and biodistribution of exosomes, which may provide an insight into the communication mechanisms and pharmacokinetics of exosomes.

Methods: Glucose modified gold nanoparticles were used to label MSC-derived exosomes, aimed at minimizing membrane damage and maintaining the integrity of the exosomes. After labeling, the exosomes were visualized by computed tomography (CT) imaging to determine the biodistribution at 4 and 24 h after injection into a MI mouse model.

Results: MSC-derived exosomes were successfully labeled by glucose modified gold nanoparticles and CT imaging of these labeled exosomes indicated that MSC-Exo remained in the MI area for up to 24 h after intramyocardial injection. Additionally, few MSC-Exo were observed in some other organs, particularly the liver, spleen, and kidney.

Conclusions: A gentle method was used for loading GNPs into exosomes, and their successful labeling without causing aggregation was verified. CT imaging revealed the retention of MSC-Exo in the MI area, indicating their usefulness for improving heart function after infarction.
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http://dx.doi.org/10.21037/atm-21-981DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039714PMC
March 2021

Incomplete Rotator Cable Did Not Cause Rotator Cuff Dysfunction in Case of Rotator Cuff Tear: A Biomechanical Study of the Relationship Between Rotator Cable Integrity and Rotator Cuff Function.

Arthroscopy 2021 Apr 1. Epub 2021 Apr 1.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China. Electronic address:

Purpose: This study seeks to evaluate the biomechanical relationship between the severity of rotator cable tears and the function of the rotator cuff.

Methods: Twelve cadaveric shoulders with intact rotator cuff, existing rotator cable, and a critical shoulder angle below 35° were included. For each shoulder, a posterosuperior rotator cuff tear (PSRCT) (model 2) in the crescent area was formed. Then anterior insertion detached (model 3), anterior insertion detached together with the middle cable tear (model 4), and the whole rotator cable tear (model 5) were subsequently created. The rotator cuff that lay above the humeral head rotation center was detached as a global tear control (model 6), along with the primitive status as the intact control (model 1). Glenohumeral abduction was initiated by simulating deltoid and remaining rotator cuff force. Functioning of the remaining rotator cuff was evaluated using the middle deltoid force (MDF), as required for abduction.

Results: No statistically significant differences in peak MDF values were seen among the 4 PSRCT statuses (44.10 ± 7.30 N [model 2], P = .96; 45.50 ± 9.55 N [model 3], P = .86; 45.90 ± 3.53 N [model 4], P = 0.30; 44.20 ± 8.19 N [model 5], P = .80) and intact control status (39.79 ± 7.65 N [model 1]). However, significant differences in peak MDF values were found among the 4 PSRCT statuses and the global tear control status (54.53 ± 7.46 N [model 6], P < .01).

Conclusion: The PSRCT, regardless of the severity of the rotator cable tear, does not induce functionally significant biomechanical impairment. Tear extension involving all rotator cuff tissue above the geometric rotation center of the humeral head results in obvious functional impairment.

Clinical Relevance: For PSRCT, the remaining rotator cuff tissue above the geometric rotation center may contribute to the preservation of shoulder function in RCT patients.
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http://dx.doi.org/10.1016/j.arthro.2021.03.051DOI Listing
April 2021

Combinatorial Synthesis of Novel 3/5(3,5)-(Di)nitro/chloropaeonol Carbonyl Hydrazone Derivatives as Nematicidal Agents.

Comb Chem High Throughput Screen 2021 Mar 24. Epub 2021 Mar 24.

Laboratory of Pesticidal Design & Synthesis, Department of Plant Protection, College of Horticultrue and Plant Protection, Henan University of Science and Technology, Luoyang 471023. China.

Background: Developing the high-efficiency and low-risk small-molecule green-nematocide is the key of effective control of the nematodes. Paeonol, is a naturally occurring phenolic compound, isolated from the root bark of Paeonia suffruticosa and the whole plant of Cynanchum paniculatum. Due to its crucial phenolic ketone skeleton, modern biological science research has indicated that paeonol has a wide range of biological activities. The structural modification of paeonol into paeonol carbonyl hydrazone derivatives is a potential approach for the development of novel nematodes, which showed more toxicity than paeonol. However, there are no reports on the nematicidal activity of paeonol carbonyl hydrazone derivatives to control Heterodera glycines.

Results: We always endeavor to discover and develop biorational natural products-based pesticidal agents, 4 significant intermediates and 21 novel 3/5(3,5)-(di)nitro/chloropaeonol carbonyl hydrazone derivatives were prepared, and their structures well characterized by 1H NMR, HRMS, MS, and mp. Due to the steric hindrance, the substituents on the C=N double bond of all hydrazine compounds adopted E configuration. Results of nematicidal activity revealed that, among all compounds, especially 5-nitropaeonol (5) and 3,5-dinitropaeonol (7) displayed the most potent nematicidal activity H. glycines in vivo with LC50 values of 0.0323 and 0.0367 mg/mL, respectively.

Conclusion: It suggested that for the 3/5(3,5)-(di)nitro/chloropaeonol carbonyl hydrazone derivatives, a nitro group introduced at C5 position of 1 was necessary for obtaining the potent compound as nematicidal agents. These preliminary results will pave the way for further modification of paeonol in the development of potential new nematicides.
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http://dx.doi.org/10.2174/1386207324666210324145627DOI Listing
March 2021

Macroporous 3D Scaffold with Self-Fitting Capability for Effectively Repairing Massive Rotator Cuff Tear.

ACS Biomater Sci Eng 2021 03 23;7(3):904-915. Epub 2020 Apr 23.

Department of Sports Medicine, Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, No. 600 Yishan Road, Shanghai 200233, PR China.

The postoperative retear rate of direct repair of massive rotator cuff tear has risen up to 40% because of the dissatisfied tendon-to-bone healing and poor regenerative potential of remnant rotator cuff tissue. A biological scaffold that connects the remnant rotator cuff tissue and bone might be a promising substitute. In the present study, we have developed a macroporous three-dimensional scaffold poly(ester-urethane)urea (PEUU), with self-fitting capability employing thermally induced phase separation (TIPS) technique. The scaffold provides oriented connected macropores for cells migration, and promoted tendon-to-bone healing on the basis of surgical repair. The scaffolds were characterized by scanning electron microscopy, stress-strain test and cell biocompatibility study. In vitro studies exhibited that PEUU scaffold with suitable elastic mechanical properties can better support proliferation and migration of rabbit bone mesenchymal stem cells (RBMSCs). After three months postreconstruction of massive rotator cuff tear in a rabbit model using PEUU scaffold, there was complete regeneration of rotator cuff with physical tendon-to-bone interface and continuous tendon tissue, as observed from histological analysis. Further, biomechanical testing demonstrated that rotator cuff induced by PEUU scaffold had no significant difference as compared to normal rotator cuff. This macroporous, mechanically matched scaffold is potentially suitable for the application in massive rotator cuff repair. In conclusion, this study demonstrates the high efficiency of the macroporous 3D scaffold with self-fitting capability in facilitating rotator cuff regeneration.
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http://dx.doi.org/10.1021/acsbiomaterials.0c00193DOI Listing
March 2021

Cervical erector spinae plane block as a perioperative analgesia method for shoulder arthroscopy: a case series.

J Anesth 2021 06 8;35(3):446-450. Epub 2021 Mar 8.

Department of Anesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.

Shoulder arthroscopy, a common intervention for severe rotator cuff injuries, is associated with severe postoperative pain. Upon performing cervical erector spinae plane (ESP) blocks at the C7 TP (tip or posterior tip) or the posterior tip of the C6 TP posterior tubercle in six patients undergoing shoulder arthroscopy, sensory block was detectable in congruent cervico-thoracic dermatomes. Effective intraoperative and postoperative analgesia were consistently obtained for all six patients. This preliminary study illustrated that the cervical ESP block can be considered a potential simple regional anesthesia method for providing analgesia during shoulder arthroscopy with low risks of diaphragmatic paresis, upper extremity motor paresis, nerve injury and persistent hypotension.
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http://dx.doi.org/10.1007/s00540-021-02907-xDOI Listing
June 2021

Synthesis of novel 3/5(3,5)-(di)nitropaeonol hydrazone derivatives as nematicidal agents.

J Asian Nat Prod Res 2021 Feb 8:1-10. Epub 2021 Feb 8.

Laboratory of Pesticidal Design & Synthesis, Department of Plant Protection, College of Forestry, Henan University of Science and Technology, Luoyang 471023, China.

Eighteen novel 3/5(3,5)-(di)nitropaeonol hydrazone derivatives were prepared, and their structures well characterized by H NMR, HRMS, and mp. Due to the steric hindrance, the substituents on the C = N double bond of all hydrazine compounds (except  = 4/1 for , , , and  = 3/2 for , ) adopted configuration. Among all compounds, four compounds , , , and exhibited potent nematicidal activity than their precursor paeonol, especially 5-nitropaeonol () and 3,5-dinitropaeonol () displayed the most potent nematicidal activity with LC values of 32.3307 and 36.7074 mg/L, respectively.
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http://dx.doi.org/10.1080/10286020.2021.1874941DOI Listing
February 2021

Controllable Synthesis of Biomimetic Hydroxyapatite Nanorods with High Osteogenic Bioactivity.

ACS Biomater Sci Eng 2020 01 6;6(1):320-328. Epub 2019 Dec 6.

The Key Laboratory for Ultrafine Materials of Ministry of Education, Engineering Research Centre for Biomedical Materials of Ministry of Education, State Key Laboratory of Bioreactor Engineering, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.

The development of biodegradable materials with high osteogenic bioactivity is important for achieving rapid bone regeneration. Although hydroxyapatite (HAp) has been applied as a biomaterial for bone engineering due to its good osteoconductivity, conventional synthetic HAp nanomaterials still lack sufficient osteogenesis, likely due to their high crystallinity and uncontrollable architecture. A design of HAp nanoparticles mimicking bone features may create good microenvironments that promote osteogenesis for rapid bone regeneration. In this study, HAp nanoparticles with a comparatively less crystalline structure and nanorod shapes mimicking biological HAp nanocrystals of natural bone were fabricated using a simple chemical precipitation approach with mild temperature control in the absence of any organic solvents. Transmission electron microscopy (TEM) indicated that HAp nanorods with aspect ratios from 2.0 to 4.4 were synthesized by adjusting the reaction time as well as the reaction temperature. Fourier transform infrared spectroscopy and X-ray diffraction experiments displayed that HAp nanorods prepared at 30 °C (HAp-30 with an aspect ratio of 2.9) had a low crystalline structure and B-type CO substitution similar to those of natural HAp originating from bone tissue. The energy-dispersive spectroscopy (EDS) results showed that the Ca/P ratio of HAp-30 was 1.66 ± 0.13. An in vitro biological evaluation against rat bone marrow-derived mesenchymal stem cells indicated that the resulting HAp nanorods had excellent biocompatibility (with an ∼80-fold increase in IC50 compared to that of conventional HAp nanoparticles). Interestingly, the alkaline phosphatase (ALP), alizarin red S, and immunofluorescence staining results all showed that stem cells display an obvious osteogenesis dependence on the HAp nanostructure. Specifically, HAp nanorods with a moderate aspect ratio had the optimal osteogenic capacity (e.g., HAp-30 offered a 2.8-fold increase in ALP expression and a 4-fold increase in OCN expression relative to that provided by irregular HAp at day 14). It is expected that HAp nanorods with controllable architectures and size have potential as a kind of new bioactive bone filler for bone defect repair.
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http://dx.doi.org/10.1021/acsbiomaterials.9b00914DOI Listing
January 2020

Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction.

ACS Biomater Sci Eng 2020 10 24;6(10):5700-5710. Epub 2020 Sep 24.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.

Anterior cruciate ligament (ACL) reconstruction with allografts is limited by high immunogenicity, poor cellularization, and delayed tendon-bone healing. Decellularized tendons (DAs) have been used as bioscaffolds to reconstruct ligaments with variable success. In the study, four kinds of decellularized allogeneic hamstring tendons were prepared and their microstructure and cytocompatibility were examined in vitro. The results showed that decellularized allografts neutralized by 5% calcium bicarbonate had typical reticular and porous microstructures with optical cytocompatibility. Tissue-engineering decellularized allografts (TEDAs) were prepared with the selected decellularized allografts and tendon stem/progenitor cells and used for ACL reconstruction in a rabbit model. Histological staining showed that the TEDAs promoted cellular infiltration and new vessel formation significantly and improved tendon-bone healing moderately compared to decellularized allografts. Better macroscopic scores and biomechanical results were observed in TEDA groups, but there were no significant differences between DA and TEDA groups at months 1, 2, and 3 postoperatively. Immunohistochemical data showed that the tissue-engineering decellularized allografts enhanced the expression of collagen I at each timepoint and collagen III at months 1 and 2. ELISA analysis showed that the tissue-engineering decellularized allografts reduced the secretion of IgE and IL-1β within 1 month and promoted the secretion of IL-2, IL-4, IL-10, and IL-17 after 1 month. The results showed that tissue-engineering decellularized allografts strengthened intra-articular graft remodeling significantly and provided moderate improvements in tendon-bone healing by creating more suitable immune responses than decellularized allografts. The study revealed that tissue-engineering decellularized allografts as a promising option for ACL reconstruction could achieve more favorable outcomes.
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http://dx.doi.org/10.1021/acsbiomaterials.0c00269DOI Listing
October 2020

What is the impact of human umbilical cord mesenchymal stem cell transplantation on clinical treatment?

Stem Cell Res Ther 2020 12 1;11(1):519. Epub 2020 Dec 1.

Anhui Key Laboratory, Department of Pharmacy, Yijishan Hospital Affiliated to Wannan Medical College, Wuhu, China.

Background: Human umbilical cord mesenchymal stem cells (HUC-MSCs) present in the umbilical cord tissue are self-renewing and multipotent. They can renew themselves continuously and, under certain conditions, differentiate into one or more cell types constituting human tissues and organs. HUC-MSCs differentiate, among others, into osteoblasts, chondrocytes, and adipocytes and have the ability to secrete cytokines. The possibility of noninvasive harvesting and low immunogenicity of HUC-MSCs give them a unique advantage in clinical applications. In recent years, HUC-MSCs have been widely used in clinical practice, and some progress has been made in their use for therapeutic purposes.

Main Body: This article describes two aspects of the clinical therapeutic effects of HUC-MSCs. On the one hand, it explains the benefits and mechanisms of HUC-MSC treatment in various diseases. On the other hand, it summarizes the results of basic research on HUC-MSCs related to clinical applications. The first part of this review highlights several functions of HUC-MSCs that are critical for their therapeutic properties: differentiation into terminal cells, immune regulation, paracrine effects, anti-inflammatory effects, anti-fibrotic effects, and regulating non-coding RNA. These characteristics of HUC-MSCs are discussed in the context of diabetes and its complications, liver disease, systemic lupus erythematosus, arthritis, brain injury and cerebrovascular diseases, heart diseases, spinal cord injury, respiratory diseases, viral infections, and other diseases. The second part emphasizes the need to establish an HUC-MSC cell bank, discusses tumorigenicity of HUC-MSCs and the characteristics of different in vitro generations of these cells in the treatment of diseases, and provides technical and theoretical support for the clinical applications of HUC-MSCs.

Conclusion: HUC-MSCs can treat a variety of diseases clinically and have achieved good therapeutic effects, and the development of HUC-MSC assistive technology has laid the foundation for its clinical application.
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http://dx.doi.org/10.1186/s13287-020-02011-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705855PMC
December 2020

A Secondary Injury of the Anterolateral Structure Plays a Minor Role in Anterior and Anterolateral Instability of Anterior Cruciate Ligament-Deficient Knees in the Case of Functional Iliotibial Band.

Arthroscopy 2021 04 20;37(4):1182-1191. Epub 2020 Nov 20.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China. Electronic address:

Purpose: To analyze the contribution of a secondary anterolateral structure (ALS) deficiency to knee instability based on anterior cruciate ligament (ACL) deficiency, in the condition of a functional iliotibial band (ITB).

Methods: Nine freshly-frozen cadaveric knees were sectioned sequentially to create ACL deficiency and ACL-ALS deficiency, using intact knees before sectioning as controls. When ITB was tensioned with 30 N, 4 separate aspects of knee instability were tested as follows: anterior translation in 90 N anterior load, isolated internal rotation in 5 N·m internal rotational torque from 0° to 90° in 15° increments, and anterolateral translation and internal rotation during a simulated pivot-shift test at 0°, 15°, 30°, and 45°. The contribution of ACL deficiency alone and additional ALS deficiency to knee instability were evaluated.

Results: The addition of an ALS lesion produced no significant exacerbation of either anterior translational or pivot shift instability in ACL-deficient knees. Additional ALS deficiency in an ACL-deficient knee resulted in a significant increase in isolated internal rotation from 45° to 90° (P = .001 at 45° and P < .001 in other cases). After sequentially sectioning, the contribution to instability of additional ALS deficiency to the entire instability in ACL-ALS-deficient knees was significantly smaller than that of ACL deficiency alone during anterior load and pivot-shift test (P < .001 in all cases), but significantly contributed more to isolated internal rotational instability at 60° (P = .011) and 90° (P = .015).

Conclusions: When ITB was tensioned, ALS played a minor role in controlling both anterior or pivot shift stability in ACL-deficient knees but a major role in restraining isolated internal rotation from 45° to 90°.

Clinical Relevance: In the condition of functional ITB, concomitant ALS injury might not exacerbate anterior and pivot-shift instability after ACL rupture, while affecting isolated internal rotation stability at higher flexion.
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http://dx.doi.org/10.1016/j.arthro.2020.10.038DOI Listing
April 2021

Radiographic Reference Points Do Not Ensure Anatomic Femoral Fixation Sites in Medial Patellofemoral Ligament Reconstruction: A Quantified Anatomic Localization Method Based on the Saddle Sulcus.

Am J Sports Med 2021 02 11;49(2):435-441. Epub 2020 Nov 11.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Background: Medial patellofemoral ligament (MPFL) reconstruction is one of the main treatments for lateral patellar translation. Based on intraoperative true lateral radiographs, the accepted methods for femoral MPFL tunnel location are potentially inaccurate. Direct assessment of anatomic characteristics during surgery through palpation of the anatomic landmarks involving the saddle sulcus might help eliminate tunnel malposition.

Hypothesis: The saddle sulcus is a reliable osseous landmark where the MPFL attaches for tunnel placement.

Study Design: Descriptive laboratory study.

Methods: A total of 9 fresh-frozen unpaired human cadaveric knees were dissected; MPFL insertion point and relative osseous structures were marked. Three-dimensional images and transformed true lateral radiographs were obtained for analysis; 3 previously reported radiographic reference points for MPFL femoral tunnel placement were determined on all images and compared with the anatomic insertion.

Results: A saddle sulcus consistently existed where the MPFL was attached, located at 11.7 ± 5.9 mm from the apex of the adductor tubercle (AT) to the medial epicondyle (ME), 62.8% of the average distance between the apexes of the AT and ME, and 5.6 ± 2.8 mm perpendicular-posterior to the border connecting the AT and ME. The reported radiographic reference points were located at average distances of 6.2 ± 3.2 mm (Schöttle method), 5.9 ± 2.3 mm (Redfern method), and 7.3 ± 6.6 mm (Fujino method) from the saddle sulcus center on the true lateral radiographs.

Conclusion: The saddle sulcus was a reliable landmark where the MPFL was anatomically attached, located approximately 12 mm from the AT to the ME (approximately 60% along a line from the AT to the ME) and 6 mm perpendicular-posterior to the border connecting the apexes of the AT and ME. Additionally, the saddle sulcus position presented variability on the femoral aspect of different knees. All of the average direct distances from the sulcus to the reference radiographic points exceeded 5 mm, and tunnel localizations on a true lateral radiograph were inaccurate.

Clinical Relevance: This study demonstrates the potential precise position of the saddle sulcus, according to the ME and AT, as a reliable anatomic landmark for MPFL femoral tunnel location. Radiographic reference points were not accurate during MPFL reconstruction. Direct palpation of the landmarks might be effective for femoral MPFL tunnel placement.
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http://dx.doi.org/10.1177/0363546520972420DOI Listing
February 2021

Design, synthesis and antibacterial evaluation of novel oxazolidinone derivatives nitrogen-containing fused heterocyclic moiety.

Bioorg Med Chem Lett 2021 01 2;32:127660. Epub 2020 Nov 2.

School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang 110016, China. Electronic address:

A series of novel oxazolidinone derivatives with nitrogen-containing fused heterocyclic moiety were designed and synthesized in this article. Their antibacterial activities were measured against S. aureus, MRSA and MSSA by MIC assay. Most of them exhibited potent activity against Gram-positive pathogens comparable to Linezolid and Radezolid. Compound 3b, which exhibited significant antibacterial activity with MIC values ranging 0.5-1.0 μg/mL, might be a promising drug candidate for further investigation.
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http://dx.doi.org/10.1016/j.bmcl.2020.127660DOI Listing
January 2021

Chemokine CXCL14 acts as a potential genetic target for liver fibrosis.

Int Immunopharmacol 2020 Dec 8;89(Pt A):107067. Epub 2020 Oct 8.

The Key Laboratory of Anti-inflammatory and Immune Medicines, Ministry of Education, Anhui Province Key Laboratory of Major Autoimmune Diseases, School of Pharmacy, Institute for Liver Disease of Anhui Medical University, Hefei, Anhui Province, China. Electronic address:

There are multiple causes of liver fibrosis, common ones include ethanol, toxins, and cholestasis. However, whether these different etiologies lead to the same pathological outcomes contain common genetic targets or signaling pathways, the current research has not attracted widespread attention. GSE40041 and GSE55747 were downloaded from the Gene Expression Omnibus (GEO) database. GSE40041 and GSE55747 represent the differential expression profiles in the liver of mice with bile duct ligation (BDL) and carbon tetrachloride (CCl4) induced liver fibrosis models, respectively. By using GEO2R, 701 differential expression genes (DEGs) in GSE40041 and 6540 DEGs in GSE55747 were identified. 260 co-DEGs were shared and extracted for gene ontology (GO) analysis. Through GO analysis, it was found that the regulation of cell migration in biological processes (BPs) was closely related to the pathogenesis of liver fibrosis, and the genes involved in this process include a key gene, chemokine (C-X-C motif) ligand 14 (CXCL14). Subsequently, further bioinformatic analysis showed that CXCL14 may be regulated by miR-122 to participate in the progression of liver fibrosis. Then real-time PCR and western blotting were performed to validate the expression of CXCL14 in liver tissue after liver fibrosis caused by different etiologies (ethanol, CCl4). The expression of CXCL4 in liver fibrosis induced by BDL was verified in another GEO dataset. Basically consistent with our bioinformatics results, our experimental results showed that the expression of CXCL14 was most significantly increased in alcoholic liver fibrosis model, followed by CCl4-induced liver fibrosis, which was also significantly increased in the BDL-induced model. Thus, CXCL14 can act as a common potential genetic target for different liver fibrosis diseases.
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http://dx.doi.org/10.1016/j.intimp.2020.107067DOI Listing
December 2020

Electrodeposition of calcium phosphate onto polyethylene terephthalate artificial ligament enhances graft-bone integration .

Bioact Mater 2021 Mar 29;6(3):783-793. Epub 2020 Sep 29.

Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.

It is a big challenge to develop a polyethylene terephthalate (PET) artificial ligament with excellent osteogenetic activity to enhance graft-bone integration for ligament reconstruction. Herein, we evaluated the effect of biomineralization (BM) and electrodeposition (ED) method for depositing calcium-phosphate (CaP) on the PET artificial ligament and . Scanning electron microscopy and energy-dispersive X-Ray spectrometer mapping analysis revealed that the ED-CaP had more uniform particles and element distribution (Ca, P and O), and thermogravimetric analysis showed there were more CaP on the PET/ED-CaP than the PET/BM-CaP scaffold. Moreover, the hydrophilicity of PET scaffolds was significantly improved after CaP deposition. study showed that CaP coating via BM or ED method could improve the attachment and proliferation of MC3T3-E1 cells, and ED-CaP coating significantly increased osteogenic differentiation of the cells, in which the Wnt/β-catenin signaling pathway might be involved. In addition, radiological, histological and immunohistochemical results of study in a rabbit anterior cruciate ligament (ACL) reconstruction model demonstrated that the PET/BM-CaP and PET/ED-CaP scaffolds significantly improved graft-bone integration process compared to the PET scaffold. More importantly, larger areas of new bone ingrowth and the formation of fibrocartilage tissue were observed at 12 weeks in the PET/ED-CaP group, and the biomechanical tests showed increased ultimate failure load and stiffness in PET/ED-CaP group compared to PET/BM-CaP and PET group. Therefore, ED of CaP is an effective strategy for the modification of PET artificial ligament and can enhance graft-bone integration both and .
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http://dx.doi.org/10.1016/j.bioactmat.2020.08.037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7527997PMC
March 2021

Intravoxel Incoherent Motion Magnetic Resonance Imaging for Assessing Parotid Gland Tumors: Correlation and Comparison with Arterial Spin Labeling Imaging.

Korean J Radiol 2021 02 10;22(2):243-252. Epub 2020 Sep 10.

Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Objective: To compare and correlate the findings of intravoxel incoherent motion (IVIM) magnetic resonance (MR) imaging and arterial spin labeling (ASL) imaging in characterizing parotid gland tumors.

Materials And Methods: We retrospectively reviewed 56 patients with parotid gland tumors evaluated by MR imaging. The true diffusion coefficient (D), pseudo-diffusion coefficient (D*), and fraction of perfusion (f) values of IVIM imaging and tumor-to-parotid gland signal intensity ratio (SIR) on ASL imaging were calculated. Spearman rank correlation coefficient, chi-squared, Mann-Whitney U, and Kruskal-Wallis tests with the post-hoc Dunn-Bonferroni method and receiver operating characteristic curve assessments were used for statistical analysis.

Results: Malignant parotid gland tumors showed significantly lower D than benign tumors ( = 0.019). Within subgroup analyses, pleomorphic adenomas (PAs) showed significantly higher D than malignant tumors (MTs) and Warthin's tumors (WTs) ( < 0.001). The D* of WTs was significantly higher than that of PAs ( = 0.031). The f and SIR on ASL imaging of WTs were significantly higher than those of MTs and PAs ( < 0.05). Significantly positive correlation was found between SIR on ASL imaging and f ( = 0.446, = 0.001). In comparison with f, SIR on ASL imaging showed a higher area under curve (0.853 vs. 0.891) in discriminating MTs from WTs, although the difference was not significant ( = 0.720).

Conclusion: IVIM and ASL imaging could help differentiate parotid gland tumors. SIR on ASL imaging showed a significantly positive correlation with f. ASL imaging might hold potential to improve the ability to discriminate MTs from WTs.
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http://dx.doi.org/10.3348/kjr.2020.0290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817638PMC
February 2021

Design, synthesis and biological evaluation of thieno[3,2-d]pyrimidine derivatives containing aroyl hydrazone or aryl hydrazide moieties for PI3K and mTOR dual inhibition.

Bioorg Chem 2020 11 28;104:104197. Epub 2020 Aug 28.

Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang 110016, China. Electronic address:

Recently, PI3K and mTOR have been regarded as promising targets for cancer treatment. Herein, we designed and synthesized four series of novel thieno[3,2-d]pyrimidine derivatives that containing aroyl hydrazone or aryl hydrazide moieties. These derivatives act as PI3K/mTOR dual inhibitors, suggesting that they can be used as cancer therapeutic agents. All compounds were tested for anti-proliferative activity against four cancer cell lines. The structure-activity relationship (SAR) studies were conducted by varying the moieties at the C-6 and C-2 positions of the thieno[3,2-d]pyrimidine core. It indicated that aryl hydrazide at C-6 position and 2-aminopyrimidine at C-2 position are optimal fragments. Compound 18b showed the most potent in vitro activity (PI3Kα IC = 0.46 nM, mTOR IC = 12 nM), as well as good inhibition against PC-3 (human prostate cancer), HCT-116 (human colorectal cancer), A549 (human lung adenocarcinoma) and MDA-MB-231 (human breast cancer) cell lines. Furthermore, Annexin-V and propidium iodide (PI) double staining confirmed that 18b induces apoptosis in cytotoxic HCT-116 cells. Moreover, the influence of 18b on cell cycle distribution was assessed on the HCT-116 cell line, and a cell cycle arrest was observed at the G/S phases.
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http://dx.doi.org/10.1016/j.bioorg.2020.104197DOI Listing
November 2020

Correction: Chondroitin sulfate-polydopamine modified polyethylene terephthalate with extracellular matrix-mimetic immunoregulatory functions for osseointegration.

J Mater Chem B 2020 Sep;8(36):8476-8477

Department of Sports Medicine, Shanghai Jiaotong University Affiliated Sixth People's Hospital, No. 600 Yishan Road, Shanghai 200233, China.

Correction for 'Chondroitin sulfate-polydopamine modified polyethylene terephthalate with extracellular matrix-mimetic immunoregulatory functions for osseointegration' by Ya-Min Li et al., J. Mater. Chem. B, 2019, 7, 7756-7770, DOI: 10.1039/C9TB01984G.
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http://dx.doi.org/10.1039/d0tb90152kDOI Listing
September 2020

Two splice variants of the DsMEK1 mitogen-activated protein kinase kinase (MAPKK) are involved in salt stress regulation in in different ways.

Biotechnol Biofuels 2020 19;13:147. Epub 2020 Aug 19.

Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610065 China.

Background: can produce glycerol under salt stress, and this production can quickly adapt to changes in external salt concentration. Notably, glycerol is an ideal energy source. In recent years, it has been reported that the mitogen-activated protein kinase cascade pathway plays an important role in regulating salt stress, and in DtMAPK can regulate glycerol synthesis under salt stress. Therefore, it is highly important to study the relationship between the MAPK cascade pathway and salt stress in and modify it to increase the production of glycerol.

Results: In our study, we identified and analysed the alternative splicing of DsMEK1 (DsMEK1-X1, DsMEK1-X2) from the unicellular green alga . DsMEK1-X1 and DsMEK1-X2 were both localized in the cytoplasm. qRT-PCR assays showed that DsMEK1-X2 was induced by salt stress. Overexpression of DsMEK1-X2 revealed a higher increase rate of glycerol production compared to the control and DsMEK1-X1-oe under salt stress. Under salt stress, the expression of DsGPDH2/3/5/6 increased in DsMEK1-X2-oe strains compared to the control. This finding indicated that DsMEK1-X2 was involved in the regulation of DsGPDH expression and glycerol overexpression under salt stress. Overexpression of DsMEK1-X1 increased the proline content and reduced the MDA content under salt stress, and DsMEK1-X1 was able to regulate oxidative stress; thus, we hypothesized that DsMEK1-X1 could reduce oxidative damage under salt stress. Yeast two-hybrid analysis showed that DsMEK1-X2 could interact with DsMAPKKK1/2/3/9/10/17 and DsMAPK1; however, DsMEK1-X1 interacted with neither upstream MAPKKK nor downstream MAPK. DsMEK1-X2-oe transgenic lines increased the expression of DsMAPKKK1/3/10/17 and DsMAPK1, and DsMEK1-X2-RNAi lines decreased the expression of DsMAPKKK2/10/17. DsMEK1-X1-oe transgenic lines did not exhibit increased gene expression, except for DsMAPKKK9.

Conclusion: Our findings demonstrate that DsMEK1-X1 and DsMEK1-X2 can respond to salt stress by two different pathways. The DsMEK1-X1 response to salt stress reduces oxidative damage; however, the DsMAPKKK1/2/3/9/10/17-DsMEK1-X2-DsMAPK1 cascade is involved in the regulation of DsGPDH expression and thus glycerol synthesis under salt stress.
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http://dx.doi.org/10.1186/s13068-020-01786-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7439689PMC
August 2020
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