Publications by authors named "Jia Chen"

1,570 Publications

  • Page 1 of 1

Monoporous Microsphere as a Dynamically Movable Drug Carrier for Osteoporotic Bone Remodeling.

Adv Healthc Mater 2022 Aug 10:e2201242. Epub 2022 Aug 10.

Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.

To repair systematically osteoporotic bone defects, it is significant to take effort on both the diminishment of osteoporosis and the enhancement of bone regeneration. Herein, a specifically monoporous microsphere carrier encapsulating dosage-sensitive and short half-time parathyroid hormone (PTH) has been constructed to tackle the issue. Compared with conventional microsphere carriers involving compact, porous, and mesoporous microspheres, the monoporous microsphere is desirable to achieve precisely in-situ delivery and to minimize topical accumulation. Our findings show that the PTH loaded inside MPMs can be gradually released from the single hole of MPMs to improve the initial drug concentration. Also, the MPMs can self-shift with the daily movement of experimental animals to effectively reduce the topical aggregation of released drugs in vitro. In vivo evaluation further confirms that the implant of MPMs-PTH plays a dual role in stimulating the regenerative repair of the cranial defect and relieving osteoporosis in the whole body. Consequently, our current work develops a dynamically movable drug delivery system to achieve precisely in-situ delivery, minimize topical accumulation, and systematically repair osteoporotic bone defects. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adhm.202201242DOI Listing
August 2022

Clinical Significance of Serum Biomarkers in Stage IV Non-Small-Cell Lung Cancer Treated with PD-1 Inhibitors: LIPI Score, NLR, dNLR, LMR, and PAB.

Dis Markers 2022 30;2022:7137357. Epub 2022 Jul 30.

Department of Medical Oncology, The Affiliated Tumor Hospital of Nantong University & Nantong Tumor Hospital, Nantong, China.

Background: To assess the prognostic value of pretreatment serum biomarkers in stage IV non-small-cell lung cancer (NSCLC) patients treated with PD-1 (programmed cell death protein 1) inhibitors and their value as a predictor of benefit.

Methods: We performed a retrospective study including patients with stage IV NSCLC who were treated with anti-PD-1 drugs in first or advanced lines of therapy in the Affiliated Tumor Hospital of Nantong University. Serum biomarkers such as NLR, dNLR, LMR, PAB, ALB, and LIPI scores were calculated and analyzed in detail.

Results: A total of 85 patients with stage IV NSCLC treated with PD-1 inhibitors in the first or advanced lines of therapy were included in this subject. According to the tumor response of PD-1-based treatment, ORR was 42.4% (36/85) and DCR was 68.2% (58/85). The median OS and PFS were 20.0 months and 7.0 months, respectively. The ROC curves showed that the serum biomarkers of NLR, dNLR, LDH, LMR, PAB, and ALB were significantly associated with overall survival and helped to determine the cut-off value. The multivariate Cox proportional hazard analyses for stage IV NSCLC patients treated with PD-1 inhibitors indicated that dNLR ( < 0.001) and ALB ( = 0.033) were independent prognostic indicators of PFS, while liver metastasis ( = 0.01), NLR ( = 0.01), dNLR ( = 0.001), and LMR ( = 0.006) were independent prognostic indicators of OS. Moreover, patients of the good LIPI group showed prolonged PFS and OS than those with intermediate/poor LIPI score ( < 0.001 and = 0.006, respectively).

Conclusions: Pretreatment dNLR is an independent prognostic indicator of both PFS and OS in stage IV NSCLC patients treated with PD-1 inhibitors. Pretreatment LIPI, combining dNLR > 3 and LDH>ULN, was correlated with worse outcome for stage IV NSCLC patients treated with ICI. High NLR, high dNLR, low LMR, and low ALB at baseline might be useful as an early predictive biomarker of benefit.
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http://dx.doi.org/10.1155/2022/7137357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357262PMC
August 2022

The role of PPARγ in intermittent hypoxia-related human umbilical vein endothelial cell injury.

Sleep Breath 2022 Aug 9. Epub 2022 Aug 9.

Department of Respiratory and Critical Care Medicine, Fujian Provincial Sleep-Disordered Breathing Clinic Center; Institute of Respiratory Disease, the First Affiliated Hospital of Fujian Medical University, Fujian Medical University, No 20, Chazhong road, Taijiang district, Fujian, 350005, Fuzhou, China.

Purpose: Patients with obstructive sleep apnoea (OSA) have a high incidence of vascular endothelial injury. The most important pathophysiological feature of OSA is chronic intermittent hypoxia (CIH). This study aimed to investigate the mechanisms of CIH-related vascular endothelial injury.

Methods: IH exposure was applied to human umbilical vein endothelial cells (HUVECs). After modeling, cell viability, the expression levels of peroxisome proliferator activated receptor γ (PPARγ), apoptosis-associated proteins and mitochondrial division fusion proteins, and the levels of reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were assessed via Cell Counting Kit-8 (CCK-8), western blotting, fluorescent microscope, and flow cytometry, respectively. Rosiglitazone (PPARγ agonist), tempo (the mitochondrial-specific antioxidant), and tempo combined with PPARγ interfering RNA were used to treat HUVECs, respectively.

Results: After IH exposure, cell viability and levels of MMP decreased, cell apoptosis and ROS levels increased, and the expression levels of PPARγ decreased. Both tempo and rosiglitazone pretreatment ameliorated cell apoptosis and improved cell viability. In addition, mitochondrial function became better after tempo pretreatment. PPARγ interference reversed the protective effects of tempo on IH-related mitochondrial function injury and cell injury.

Conclusions: PPARγ regulated the apoptosis and cell viability of IH-treated HUVECs by altering mitochondrial function. This finding clarifies the mechanism of CIH-related vascular endothelial injury.
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http://dx.doi.org/10.1007/s11325-022-02696-xDOI Listing
August 2022

N-acetylcysteine as prophylactic therapy for transplant-associated thrombotic microangiopathy: a randomized, placebo-controlled trial.

Transplant Cell Ther 2022 Aug 5. Epub 2022 Aug 5.

National clinical research center for hematologic diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China; Institute of Blood and Marrow Transplantation, Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China; Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, Suzhou, China; State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China. Electronic address:

Background: Transplant-associated thrombotic microangiopathy (TA-TMA) is a life-threatening complication for patients undergoing hematopoietic stem cell transplantation (HSCT). N-acetylcysteine (NAC) has recently been considered as a potential treatment for patients with thrombotic thrombocytopenic purpura.

Objectives: To assess the value of NAC for the prevention of TA-TMA, we conducted a prospective study at the First Affiliated Hospital of Soochow University.

Study Design: This open-label, randomized placebo-controlled trial included 160 patients who were scheduled to receive allogeneic HSCT. Participants were randomly assigned 1:1 to either oral NAC (50 mg/kg daily from 9 days before HSCT to 30 days after HSCT) or placebo treatment. The primary outcome was the incidence of TA-TMA. Overall survival (OS) and event-free survival (EFS) were assessed between NAC and placebo cohorts.

Results: The incidence of TA-TMA in the NAC cohort was 9.1% (95% confidence interval (CI), 0.02-0.162), compared with 23% (95% CI, 0.132-0.328) in placebo cohort, with a rate ratio of 0.34 (95% CI, 0.123-0.911; p=0.039). The median time to onset of TA-TMA was 60 (interquartile range [IQR] 42-129) and 36 (IQR 30.5-51) days in the NAC and control groups, respectively (p=0.063). The 2-year rates of OS in the NAC and placebo groups were 75.4% (95% CI, 2.865-7.353) and 63.0% (95% CI, 0.508-0.735), respectively, with a hazard ratio (HR) of 0.622 (95% CI, 0.334-1.155; p=0.132). The EFS rate was 25.8% in NAC patients and 8.1% in placebo patients, with an HR of 0.254 (95% CI, 0.094-0.692; p=0.024). The median time of EFS was 60 and 38 days in NAC and placebo cohorts.

Conclusion: Our findings suggest that NAC may be a potential treatment to reduce TA-TMA incidence.
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http://dx.doi.org/10.1016/j.jtct.2022.07.029DOI Listing
August 2022

Dipole Switching by Intramolecular Electron Transfer in Single-Molecule Magnetic Complex [MnO(OCR)(HO)].

J Phys Chem A 2022 Aug 8. Epub 2022 Aug 8.

The M2QM Center and the Quantum Theory Project, Department of Physics, University of Florida, Gainesville, Florida 32611, United States.

We study intramolecular electron transfer in the single-molecule magnetic complex [MnO(OCR) (HO)] for R = -H, -CH, -CHCl, -CH, and -CHF ligands as a mechanism for switching of the molecular dipole moment. Energetics is obtained using the density functional theory (DFT) with onsite Coulomb energy correction (DFT + ). Lattice distortions are found to be critical for localizing an extra electron on one of the easy sites on the outer ring in which localized states can be stabilized. We find that the lowest-energy path for charge transfer is for the electron to go through the center via superexchange-mediated tunneling. The energy barrier for such a path ranges from 0.4 to 54 meV depending on the ligands and the isomeric form of the complex. The electric field strength needed to move the charge from one end to the other, thus reversing the dipole moment, is 0.010.04 V/Å.
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http://dx.doi.org/10.1021/acs.jpca.2c02585DOI Listing
August 2022

Evaluation of the Effect of Integrated Care on Preoperative Anxiety in Day Surgery Patients Based on the MVPA Framework.

Comput Math Methods Med 2022 27;2022:2154599. Epub 2022 Jul 27.

Hunan Provincial People's Hospital Nursing Department, Changsha, Hunan 410003, China.

Objective: To investigate the effect of receiving comprehensive care on preoperative anxiety in patients admitted to the operating room at different times.

Methods: A generative model was applied to a patient's preoperative anxiety aid diagnostic model and used to address preoperative anxiety due to the patient. Group ICA first constructed a whole-brain dynamic functional connectivity network, and an initial dataset was constructed by Kendall's ranking correlation coefficient method; then, an expanded dataset was constructed by a conditional deep convolutional generative adversarial network (CDCGAN), and finally, a dynamic classification study of patients with preoperative anxiety and healthy subjects was conducted by feature selection and discriminant models in turn. Seventy patients who entered the operating theatre at different times were retrospectively analyzed. Children who entered the operating theatre after 14:00 were included in the control group ( = 35), and those who entered between 12:00 were included in the observation group ( = 35). Patients in both groups were given comprehensive care, and preoperative anxiety was compared between the two groups.

Results: The earlier the patients entered the operating room, the lower the preoperative anxiety.

Conclusion: Comprehensive care could reduce the patients' preoperative anxiety, keep their emotions stable, and improve their compliance with treatment.
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http://dx.doi.org/10.1155/2022/2154599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348959PMC
August 2022

Distinct non-clock-like signatures of the basal cell carcinomas from three sisters with a lethal Gorlin-Goltz syndrome.

BMC Med Genomics 2022 08 5;15(1):172. Epub 2022 Aug 5.

Department of Dermatology, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Background: Gorlin-Goltz syndrome (GS) is an inherited disease characterized by predisposition to basal cell carcinomas (BCCs) and various developmental defects, whose numerous disease-causing PTCH1 mutations have been identified in the hedgehog (Hh) signaling pathway.

Methods: In this study, whole exome sequencing was used to screen for both somatic and germline deleterious mutations in three sisters with a lethal GS. The mutations we found were confirmed by subcloning and Sanger sequencing of the genomic DNA. RNA-seq was performed to profile gene expression in paired BCCs samples and the expression levels for selected genes were validated by quantitative PCR.

Results: The clinical and histopathologic features were analyzed for the proband in the three-generation GS family. We identified the insertion mutation PTCH1 c.1341dupA (p. L448Tfs*49), which segregated with BCC phenotype and contributed to the death of two in four patients from a Chinese family with GS. Compared with adjacent non-cancerous tissues (ANCT), four second-hit mutations were found in four of the six pairs of BCC from three patients. Of note, somatic genomic alterations in all six BCC samples were mainly clustered into non-clock-like Signature 7 (ultraviolet mutagenesis) and 11 (related to certain alkylating agents). Both RNA-seq and quantitative RT-PCR confirmed that the mRNA levels of PTCH1 and its effector GLI1 were markedly upregulated in six pairs of BCC samples versus ANCT.

Conclusions: The distinct non-clock-like signatures of BCCs indicated that GS was not a life-threatening illness. The main reasons for untimely death of GS patients were PTCH1 mutation, exposure to intense ultraviolet radiationand the poor economic conditions.
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http://dx.doi.org/10.1186/s12920-022-01324-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354412PMC
August 2022

Corrigendum to "Hormone sensitive lipase ablation promotes bone regeneration" [Biochim. Biophys. Acta Mol. Basis Dis. Volume 1868, Issue 9, 1 September 2022, 166449].

Biochim Biophys Acta Mol Basis Dis 2022 Aug 1;1868(11):166506. Epub 2022 Aug 1.

Division of Endocrinology, Gerontology, and Metabolism, Stanford University, Stanford, CA, USA; Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Stanford Diabetes Research Center, Stanford, CA, USA. Electronic address:

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http://dx.doi.org/10.1016/j.bbadis.2022.166506DOI Listing
August 2022

FERONIA is involved in the phototropin 1-mediated blue light phototropic growth in Arabidopsis.

J Integr Plant Biol 2022 Aug 4. Epub 2022 Aug 4.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, and Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, Hunan University, Changsha, 410082, China.

Plant shoot phototropism is triggered by the formation of a light-driven auxin gradient leading to bending growth. The blue light receptor phototropin 1 (phot1) senses light direction, but how this leads to auxin gradient formation and growth regulation remains poorly understood. Previous studies have suggested phot1's role for regulated apoplastic acidification, but its relation to phototropin and hypocotyl phototropism is unclear. Herein, we show that blue light can cause phot1 to interact with and phosphorylate FERONIA (FER), a known cell growth regulator, and trigger downstream phototropic bending growth in Arabidopsis hypocotyls. fer mutants showed defects in phototropic growth, similar to phot1/2 mutant. FER also interacts with and phosphorylates phytochrome kinase substrates (PKSs), the phot1 downstream substrates. The phot1-FER pathway acts upstream of apoplastic acidification and the auxin gradient formation in hypocotyl under lateral blue light, both of which are critical for phototropic bending growth in hypocotyls. Our study highlights a pivotal role of FER in the phot1-mediated phototropic cell growth regulation in plants. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/jipb.13336DOI Listing
August 2022

Global Trends in Nursing-Related Research on COVID-19: A Bibliometric Analysis.

Front Public Health 2022 18;10:933555. Epub 2022 Jul 18.

Xiangya Nursing School, Central South University, Changsha, China.

Background: Coronavirus disease 2019 (COVID-19) has significantly impacted healthcare, especially the nursing field. This study aims to explore the current status and hot topics of nursing-related research on COVID-19 using bibliometric analysis.

Methods: Between 2019 and 2022, publications regarding nursing and COVID-19 were retrieved from the Web of Science core collection. We conducted an advanced search using the following search query string: TS = ("Novel coronavirus 2019" or "Coronavirus disease 2019" or "COVID 19" or "2019-nCOV" or "SARS-CoV-2" or "coronavirus-2") and TS = ("nursing" or "nurse" or "nursing-care" or "midwife"). Bibliometric parameters were extracted, and Microsoft Excel 2010 and VOSviewer were utilized to identify the largest contributors, including prolific authors, institutions, countries, and journals. VOSviewer and CiteSpace were used to analyze the knowledge network, collaborative maps, hotspots, and trends in this field.

Results: A total of 5,267 papers were published between 2020 and 2022. The findings are as follows: the USA, China, and the UK are the top three prolific countries; the University of Toronto, the Harvard Medical School, the Johns Hopkins University, and the Huazhong University of Science & Technology are the top four most productive institutions; Gravenstein, Stefan, and White, Elizabeth M. from Brown University (USA) are the most prolific authors; The International Journal of Environmental Research and Public Health is the most productive journal; "COVID-19," "SARS-CoV-2," "nurse," "mental health," "nursing home," "nursing education," "telemedicine," "vaccine-related issues" are the central topics in the past 2 years.

Conclusion: Nursing-related research on COVID-19 has gained considerable attention worldwide. In 2020, the major hot topics included "SARS-CoV-2," "knowledge," "information teaching," "mental health," "psychological problems," and "nursing home." In 2021 and 2022, researchers were also interested in topics such as "nursing students," "telemedicine," and "vaccine-related issues," which require further investigation.
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http://dx.doi.org/10.3389/fpubh.2022.933555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339968PMC
August 2022

α-FeO based nanotherapeutics for near-infrared/dihydroartemisinin dual-augmented chemodynamic antibacterial therapy.

Acta Biomater 2022 Jul 30. Epub 2022 Jul 30.

Guangdong Key Laboratory for Research and Development of Natural Drugs, School of Pharmacy, Guangdong Medical University, Zhanjiang 524023, China; The Marine Biomedical Research Institute of Guangdong, Zhanjiang 524023, China. Electronic address:

Due to the negligible bacterial resistance, chemodynamic therapy (CDT) is a promising treatment for bacterial infection. However, it is severely impeded by the constant body temperature, shortage of Fe(Ⅱ) ions and insufficient HO level in infected tissue. To enhance the therapeutic efficiency of CDT, improved strategies are urgently needed to tackle these problems. Herein, we exploited an infection microenvironment-responsive nanotherapeutics for near-infrared (NIR)/dihydroartemisinin (DHA) dual-augmented antibacterial CDT. The convenient encapsulation of DHA-loaded α-FeO nanorods with metal-polyphenol networks (MPN) led to the generation of an antibacterial nanoagent [email protected]@MPN (FDM). Afterwards, its photothermal and peroxidase-like activities were intensively studied. Furthermore, the bactericidal efficacy of FDM was evaluated through both in vitro and in vivo antibacterial assays. Firstly, FDM showed both satisfactory photothermal and NIR/DHA dual-augmented peroxidase-like activities. Besides, it exhibited a pH-responsive release behavior of both Fe(Ⅱ) ions and DHA. Moreover, it presented tannic acid-mediated bacterial adhesion effect. In vitro experiments demonstrated that FDM could achieve a satisfactory efficiency against both planktonic bacteria and biofilms. In vivo assays illustrated both the extraordinary synergistic antibacterial effect and efficient anti-inflammatory ability of FDM. The outcomes indicated that the exploited antibacterial agent could offer new insight on developing intelligent nanotherapeutics for clinical use in the future. STATEMENT OF SIGNIFICANCE: The antibacterial efficiency of chemodynamic therapy (CDT) is seriously limited by the constant body temperature, shortage of Fe(Ⅱ) ions and insufficient HO level at the mildly acidic inflammatory microenvironment. To address these issues, we have developed a pH-responsive nanoagent ([email protected]@MPN) for near-infrared (NIR)/dihydroartemisinin (DHA) dual-augmented CDT. Through the NIR-induced photothermal effect of exterior Fe(Ⅲ)/tannic acid complex, the increased local temperature led to a photothermal enhanced CDT. Besides, a continuous supply of Fe(Ⅱ) ions could be achieved by tannic acid-mediated Fe(Ⅲ) reduction. Moreover, DHA was adopted as a substitute for HO to initiate DHA-mediated CDT. Both in vitro and in vivo assays demonstrated its outstanding bactericidal efficiency. Therefore, the developed nanotherapeutics could be a promising candidate for clinical trials.
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http://dx.doi.org/10.1016/j.actbio.2022.07.047DOI Listing
July 2022

Application of Embedded Smart Wearable Device Monitoring in Joint Cartilage Injury and Rehabilitation Training.

J Healthc Eng 2022 7;2022:4420870. Epub 2022 Jan 7.

Operating Room, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China.

Joint injuries cause varying degrees of damage to joint cartilage. The purpose of this paper is to study the application of embedded smart wearable device monitoring in articular cartilage injury and rehabilitation training. This paper studies what an embedded system is and what a smart wearable device is and also introduces the rehabilitation training method of articular cartilage injury. We cited an embedded matching cost algorithm and an improved AD-Census. The joint cartilage damage and rehabilitation training are monitored. Finally, we introduced the types of smart wearable devices and different types of application fields. The results of this paper show that, after an articular cartilage injury, the joint function significantly recovers using the staged exercise rehabilitation training based on embedded smart wearable device monitoring. We concluded that, from 2013 to 2020, smart wearable devices are very promising in the medical field. In 2020, the value will reach 20 million US dollars.
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http://dx.doi.org/10.1155/2022/4420870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338741PMC
August 2022

Electrochemical Quantitation of the Glycosylation Level of Serum Neurofilament Light Chain for the Diagnosis of Neurodegeneration: An Interface-Solution Dual-Path Amplification Strategy.

Anal Chem 2022 Aug 1. Epub 2022 Aug 1.

Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P. R. China.

Serum neurofilament light chain (NFL), a potential general biomarker for neurodegenerative diseases, is not specific enough to differentiate neurodegenerative diseases from other brain diseases such as cerebral thrombosis (CT). According to the importance of glycosylation in neurodegenerative pathogenesis, the NFL glycosylation level (oNFL/tNFL), defined as the ratio of glycosylated NFL (oNFL) to total NFL (tNFL), may be a more effective index. The major challenge in serum oNFL/tNFL detection is the ultra-low abundance of both NFL forms. In this paper, we achieved a convenient one-step electrochemical quantitation of oNFL/tNFL based on an interface-solution dual-path amplification strategy. Two amplified electrochemical signals─the reduction of Cu from adsorbed porous nanoparticles on the sensor interface and the reduction of O from horseradish peroxidase-catalyzed HO disproportionation in solution─were adopted to quantify tNFL and oNFL, respectively. The electrochemical sensor displayed good sensitivity, selectivity, and reproducibility. The dynamic range is 1-25 pg mL for tNFL and 0.25-25 pg mL for oNFL, respectively. By analyzing the clinic serum samples, for the first time, our work provided the abundance of oNFL in human serum and revealed that the oNFL/tNFL is effective not only in differentiating three kinds of brain damage patients from healthy people but also in differentiating neurodegeneration from non-neurodegeneration CT patients. As a general biomarker, the oNFL/tNFL is more specific than NFL, which is hoped to be a new and valid indicator for the diagnosis, progression, prediction, and treatment evaluation of neurodegenerative diseases.
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http://dx.doi.org/10.1021/acs.analchem.2c02753DOI Listing
August 2022

Mass Transfer Printing of Metal-Halide Perovskite Films and Nanostructures.

Adv Mater 2022 Jul 30:e2203529. Epub 2022 Jul 30.

CAS Key Laboratory of Strongly-coupled Quantum Matter Physics, Department of Physics, University of Science and Technology of China, Hefei, Anhui, 230026, China.

Most methods of depositing perovskite films cannot meet the diverse requirements of real applications such as depositing films on various types of substrates, making patterns with different bandgaps for full-color display. Here, a robust mass transfer method of perovskite films and nanostructures is reported, meeting those requirements, by using an ultrathin branched polyethylenimine as interfacial chemical bonding layers. The transfer-printed perovskite films exhibit comparable morphology, composition, optoelectronic properties, and device performances with the counterparts made by optimized spin-coating methods. The perovskite light-emitting diodes (PeLEDs) using the transfer-printed films show decent external quantum efficiencies of 10.5% and 6.7% for red (680 nm) and sky-blue (493 nm) emissions, which are similar to the devices made by spin-coating. This robust transfer printing method also enables the the preparation of perovskite micropatterns with a high resolution up to 1270 pixels per inch. Horizontally aligned red and sky-blue perovskite microstripes are further obtained through multiple printing processes for white PeLEDs. This work demonstrates a feasible strategy for making perovskite films or micropatterns on various substrates for real applications in full-color display, white LEDs, lasing, etc.
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http://dx.doi.org/10.1002/adma.202203529DOI Listing
July 2022

Fabrication and characterization of oleogels and temperature-responsive water-in-oil emulsions based on candelilla (Euphorbia cerifera) wax.

Food Chem 2022 Jul 13;397:133677. Epub 2022 Jul 13.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address:

Developing novel fats with zero trans and low saturated fatty acids represents a research hotspot in the colloid field today. Herein, natural candelilla (Euphorbia cerifera) wax was used as an oleogelator to construct oleogel systems, and can make strong oleogels at low concentrations (3 wt%). These oleogels were further employed as continuous phases to fabricate surfactant-free W/O emulsions with excellent stability (at least 30 days). Microstructural observation confirmed that the stability of emulsions was attributed to the interface and bulk phase crystallization of wax. All oleogels and emulsions were pseudoplastic fluids whose gel properties could be tuned via regulating oleogelator concentration. Water content also influenced the emulsion rigidity, denoting the droplets acted as "active fillers". Additionally, the emulsions displayed a temperature-responsive property, beneficial in mimicking the "fat-like" melt-in-the-mouth effect. These findings greatly enrich the formulation of surfactant-free W/O emulsions, providing technical support for the development of novel fats.
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http://dx.doi.org/10.1016/j.foodchem.2022.133677DOI Listing
July 2022

Identification evidence unraveled by strict proteomics rules toward forensic samples.

Electrophoresis 2022 Jul 30. Epub 2022 Jul 30.

State Key Laboratory of Toxicology and Medical Countermeasures, and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, 100850, P. R. China.

Snake venom is a complex mixture of proteins and peptides secreted by venomous snakes from their poison glands. While proteomics for snake venom composition, interspecific differences, and developmental evolution has been developed for a decade, current diagnosis or identification techniques of snake venom in clinical intoxication and forensic science applications are mainly dependent on morphological and immunoassay. It could be expected the proteomics techniques directly offer great help. This work applied a bottom-up proteomics method to identify proteins' types and species attribution in suspected snake venom samples using ultra-high-performance liquid chromatography-quadrupole-electrostatic field Orbitrap tandem mass spectrometry technique, and cytotoxicity assay was amended to provide direct evidence of toxicity. Toward the suspicious samples seized in the security control, sample pretreatment (in sol and in-gel digestion) and data acquisition (non-target and targeted screening) modes complemented and validated each other. We have implemented two consequent approaches in identifying the species source of proteins in the samples via the points of venom proteomics and strict forensic identification. Firstly, we completed a workflow consisting of a proteomics database match toward an entire Swiss-Prot (date 20181122) database and a result-directed specific taxonomy database. The latter was a helpful hint to compare master protein kinds and reveal the insufficiency of specific venom proteomics characterization rules. Secondly, we suggested strict rules for protein identification to meet the requirements of forensic science on improved identification correctness, i.e., (1) Peptide spectrum matches confidence, peptide confidence, and protein confidence were both high (with the false-discovery ratio less than 1%); (2) the number of unique peptides was more than or equal to two in one protein, and (3) within unique peptides which at least 75% of the ∆m/z of the matched y and b ions were less than 5 ppm. We identified these samples as cobra venom containing ten highly abundant proteins (P00597, P82463, P60770, Q9YGI4, P62375, P49123, P80245, P60302, P01442, and P60304) from two snake venom protein families (acid phospholipase A2 and three-finger toxins), and the most abundant proteins were cytotoxins. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/elps.202200051DOI Listing
July 2022

Human placental microRNAs dysregulated by cadmium exposure predict neurobehavioral outcomes at birth.

Pediatr Res 2022 Jul 29. Epub 2022 Jul 29.

Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, USA.

Background: Prenatal cadmium (Cd) exposure has been implicated in both placental toxicity and adverse neurobehavioral outcomes. Placental microRNAs (miRNAs) may function to developmentally program adverse pregnancy and newborn health outcomes in response to gestational Cd exposure.

Methods: In a subset of the Rhode Island Child Health Study (RICHS, n = 115) and the New Hampshire Birth Cohort Study (NHBCS, = 281), we used small RNA sequencing and trace metal analysis to identify Cd-associated expression of placental miRNAs using negative binomial generalized linear models. We predicted mRNAs targeted by Cd-associated miRNAs and relate them to neurobehavioral outcomes at birth through the integration of transcriptomic data and summary scores from the NICU Network Neurobehavioral Scale (NNNS).

Results: Placental Cd concentrations are significantly associated with the expression level of five placental miRNAs in NHBCS, with similar effect sizes in RICHS. These miRNA target genes overrepresented in nervous system development, and their expression is correlated with NNNS metrics suggestive of atypical neurobehavioral outcomes at birth.

Conclusions: Gestational Cd exposure is associated with the expression of placental miRNAs. Predicted targets of these miRNAs are involved in nervous system development and may also regulate placental physiology, allowing their dysregulation to modify developmental programming of early life health outcomes.

Impact: This research aims to address the poor understanding of the molecular mechanisms governing adverse pregnancy and newborn health outcomes in response to Gestational cadmium (Cd) exposure. Our results outline a robust relationship between Cd-associated placental microRNA expression and NICU Network Neurobehavioral Scales (NNNS) at birth indicative of atypical neurobehavior. This study utilized healthy mother-infant cohorts to describe the role of Cd-associated dysregulation of placental microRNAs as a potential mechanism by which adverse neurobehavioral outcomes are developmentally programmed.
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http://dx.doi.org/10.1038/s41390-022-02201-wDOI Listing
July 2022

Characterization, Hypoglycemic Activity, and Antioxidant Activity of Methanol Extracts From : and Studies.

Front Nutr 2022 12;9:869749. Epub 2022 Jul 12.

Shijiazhuang Food Engineering Technology Research Center, School of Chemical Engineering, Shijiazhuang University, Shijiazhuang, China.

The dried fruit of is well-known as a spice as well as a Chinese traditional herb. This study aimed to identify the bioactive constituents in the powder of methanol extract from (PMEAT) and to evaluate the hypoglycemic and antioxidant effects of PMEAT, and . We identified 36 phytochemicals in PMEAT by employing HPLC-MS/MS. PMEAT solution was found to have potent α-glucosidase-inhibiting activity (IC, 0.145 mg/mL) , twice as strong as that of acarbose (IC, 0.273 mg/mL). To investigate the hypoglycemic activity of PMEAT , we studied the impact of low-dose PMEAT (the addition of 100 mg/kg PMEAT to the mice diet) and high-dose PMEAT (200 mg/kg PMEAT addition) treatments in STZ-induced diabetic mice. After 6 weeks of intervention, significantly decreased fasting blood glucose (FBG) ( < 0.05), significantly decreased area under the curve (AUC) of the oral glucose tolerance test ( < 0.05), significantly decreased HOMA-IR ( < 0.05), and significantly increased HOMA-β ( < 0.05) were observed in the high-dose PMEAT group. Moreover, we performed an antioxidant activity experiment . The results showed that PMEAT had a strong ability to scavenge DPPH (IC, 0.044 mg/mL) as well as ABTS free radicals (IC, 0.040 mg/mL). In an animal experiment conducted on oxidative damage mice model which was induced by D-glucose and a high-fat diet, we observed significantly increased dismutase (SOD) ( < 0.01), glutathione (GSH) ( < 0.01), and glutathione peroxidase (GSH-Px) ( < 0.01) and significantly reduced malondialdehyde (MDA) and 8-ISO-prostaglandin-PGF2α (8-ISO-PGF2α), after treatment with PMEAT for 90 days. In conclusion, this study reveals the therapeutic potential of for the treatment of diabetes and helps us discover new antioxidant candidates from natural sources.
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http://dx.doi.org/10.3389/fnut.2022.869749DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315379PMC
July 2022

MODECP: A Multi-Objective Based Approach for Solving Distributed Controller Placement Problem in Software Defined Network.

Sensors (Basel) 2022 Jul 22;22(15). Epub 2022 Jul 22.

The School Electronic and Information Engineering, Beijing Jiaotong University, Beijing 100044, China.

Software-Defined Network is an emerging networking paradigm that enables intelligent and flexible network management. Specifically, the design of the control plane is crucial. Therefore, in order to avoid a single point of failure, multiple controllers are deployed constantly in a distributed manner on the control plane. In this paper, we propose a controller placement approach based on multiple objectives (MODECP), including network delay, network security, load-balancing rate, and link occupancy. In the controller placement stage, an improved multi-objective differential evolution algorithm is proposed to search for controllers' positions and assign switches to controllers reasonably. Furthermore, an improved affinity propagation algorithm is proposed to obtain the number of controllers placed in the network partition stage, comprehensively considering the delay, node security, and load. Simulations are performed based on several topologies from Internet Topology Zoo. Extensive results show that the proposed algorithm can realize trade-offs among multiple objectives and improve network performance in delay, security, controller load, and link occupancy compared to the single-objective based approach. Moreover, compared with the genetic algorithm and random placement algorithm, the proposed algorithm performs better with low latency, high security, low load rate, and low link overhead.
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http://dx.doi.org/10.3390/s22155475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331998PMC
July 2022

Applications of Nano Hydroxyapatite as Adsorbents: A Review.

Nanomaterials (Basel) 2022 Jul 6;12(14). Epub 2022 Jul 6.

Faculty of Medicine, Sabaragamuwa University of Sri Lanka, Belihuloya 70140, Sri Lanka.

Nano hydroxyapatite (Ca(PO)(OH), HAp) has aroused widespread attention as a green and environmentally friendly adsorbent due to its outstanding ability in removing heavy metal ions, radio nuclides, organic pollutants and fluoride ions for wastewater treatment. The hexagonal crystal structure of HAp supports the adsorption mechanisms including ionic exchange reaction, surface complexation, the co-precipitation of new partially soluble phases and physical adsorption such as electrostatic interaction and hydrogen bonding. However, nano HAp has some drawbacks such as agglomeration and a significant pressure drop during filtration when used in powder form. Therefore, instead of using nano HAp alone, researchers have worked on modificationsand composites of nano HAp to overcome these issues and enhance the adsorption capacity. The modification of cationic doping and organic molecule grafting for nano HAp can promote the immobilization of ions and then increase adsorption capacity. Developing nano HAp composite with biopolymers such as gelatin, chitosan and chitin has proven to obtain a synergetic effect for improving the adsorption capacity of composites, in which nano HAp fixed and dispersed in polymers can playmuch more of a role for adsorption. This review summarizes the adsorption properties and adsorbent applications of nano HAp as well as the methods to enhance the adsorption capacity of nano HAp.
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http://dx.doi.org/10.3390/nano12142324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319406PMC
July 2022

Post-Translational Modifications in Atopic Dermatitis: Current Research and Clinical Relevance.

Front Cell Dev Biol 2022 7;10:942838. Epub 2022 Jul 7.

Shanghai Skin Disease Hospital of Tongji University, Shanghai, China.

Atopic dermatitis (AD) is a chronic and relapsing cutaneous disorder characterized by compromised immune system, excessive inflammation, and skin barrier disruption. Post-translational modifications (PTMs) are covalent and enzymatic modifications of proteins after their translation, which have been reported to play roles in inflammatory and allergic diseases. However, less attention has been paid to the effect of PTMs on AD. This review summarized the knowledge of six major classes (including phosphorylation, acetylation, ubiquitination, SUMOylation, glycosylation, o-glycosylation, and glycation) of PTMs in AD pathogenesis and discussed the opportunities for disease management.
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http://dx.doi.org/10.3389/fcell.2022.942838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301047PMC
July 2022

Current progress on innovative pest detection techniques for stored cereal grains and thereof powders.

Food Chem 2022 Jul 16;396:133706. Epub 2022 Jul 16.

College of Food Science, Southwest University, Chongqing 400715, People's Republic of China; Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, People's Republic of China. Electronic address:

For stored grains and their powders, pest infestation has always been a knotty problem and thus comprises a serious threat to global food security. Obviously, timely, rapid and accurate pest detection methods are of extreme importance to protect grains from pest mouth. In facing the defects of traditional methods, such as visual inspection, grain flotation and pest trap, diverse innovative approaches progressed fast alternatively, either targeting pest itself or diagnosing pest-induced changes. The former includes machine vision, metabolite analysis, pest-specific protein techniques, molecular techniques, bioacoustics analysis, conductive roller mill, low-field nuclear magnetic resonance spectroscopy and imaging, while the latter consists of thermal imaging, near-infrared spectroscopy and hyperspectral imaging, impact acoustics analysis, soft X-ray imaging and tomography. The principle, operation procedure, pros and cons and application scenarios were discussed for each method. The results herein hope to promote the technical revolution of pest inspection in stored cereal grains and their powders.
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http://dx.doi.org/10.1016/j.foodchem.2022.133706DOI Listing
July 2022

Upregulation of HIF-1α contributes to complement activation in transplantation-associated thrombotic microangiopathy.

Br J Haematol 2022 Jul 21. Epub 2022 Jul 21.

National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Transplantation-associated thrombotic microangiopathy (TA-TMA) is a severe complication of haematopoietic stem cell transplantation (HSCT). Complement activation is involved in the development of TA-TMA. However, the underlying mechanism is unclear. Therefore, 21 samples of TA-TMA and 1:1 matched controls were measured for hypoxia-inducible factor-1α (HIF-1α) and complement protein. The mechanism was investigated both in vitro and in vivo. In this study, we found that levels of HIF-1α were significantly higher in TA-TMA patients than that in non-TA-TMA controls. Upregulation of HIF-1α induced an increase in membrane-bound complement C3 and dysfunction of human umbilical vein endothelial cells (HUVECs) in vitro. Increasing HIF-1α in vivo led to C3 and C5b-9 deposition in the glomerular endothelial capillary complex, thrombocytopenia, anaemia, and increased serum lactate dehydrogenase (LDH) levels in wild-type (WT) but not in C3 mice subjected to HSCT. High platelet aggregation in peripheral blood and CD41-positive microthrombi in the kidney were also found in dimethyloxallyl glycine (DMOG)-treated mice, recapitulating the TA-TMA phenotype seen in patients. Comprehensive analysis, including DNA array, luciferase reporter assay, chromatin immunoprecipitation (ChIP)-seq, and quantitative polymerase chain reaction (PCR), revealed that HIF-1α interacted with the promoter of complement factor H (CFH) to inhibit its transcription. Decreased CFH led to complement activation in endothelial cells.
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http://dx.doi.org/10.1111/bjh.18377DOI Listing
July 2022

Discovery, preparation and characterization of lipid-lowering alkylphenol derivatives from Syzygium jambos fruit.

Food Chem 2022 Jul 13;396:133668. Epub 2022 Jul 13.

Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang 110169, PR China. Electronic address:

The chemical characteristics and hypolipidemic effects of alkylphenols in the fruit of Syzygium jambos were investigated in this study. Three cardanols (1-3; 1 as a new compound) and three alkylresorcinols (4-6) were isolated and identified from S. jambos fruit. Cardanols 1 and 2 (10-40 μM) suppressed lipids accumulation and reduced triglyceride content in oleic acid-overloaded HepG2 cells via the activation of AMPK/PPARα signaling pathways. Furthermore, the biological distribution of cardanols after an oral intake in mice was investigated. Compound 2 was detected in mice plasma, feces, and adipose tissues after a single oral intake (80 mg/kg body weight). In addition, an alkylphenols-enriched S. jambos fruit extract containing two bioactive compounds (95.9 and 198.6 μg/mg of compounds 1 and 2, respectively) was prepared. Findings from the current study highlight the potential usage of cardanols as well as S. jambos fruit for the management of dyslipidemia.
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http://dx.doi.org/10.1016/j.foodchem.2022.133668DOI Listing
July 2022

Epithelial Cell Polarity During Midgut Development.

Front Cell Dev Biol 2022 30;10:886773. Epub 2022 Jun 30.

Gurdon Institute and the Department of Genetics, University of Cambridge, Cambridge, United Kingdom.

The adult midgut epithelium is derived from a group of stem cells called adult midgut precursors (AMPs) that are specified during the migration of the endoderm in early embryogenesis. AMPs are maintained and expanded in AMP nests that lie on the basal side of the larval midgut throughout the larval development. During metamorphosis, the larval midgut undergoes histolysis and programmed cell death, while the central cells in the AMP nests form the future adult midgut and the peripheral cells form the transient pupal midgut. Here we review what is known about how cells polarise in the embryonic, larval, pupal and adult midgut, and discuss the open questions about the mechanisms that control the changes in cell arrangements, cell shape and cell polarity during midgut development.
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http://dx.doi.org/10.3389/fcell.2022.886773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281564PMC
June 2022

Langerhans cell histiocytosis in adolescent patients: a single-centre retrospective study.

Orphanet J Rare Dis 2022 07 15;17(1):268. Epub 2022 Jul 15.

Department of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Langerhans cell histiocytosis (LCH) is a myeloid dendritic cell disorder frequently affecting children more than adults. The presentation of LCH varies with age, however, the clinical characteristics and genetic profiles of adolescent LCH remain elusive. To address the knowledge gap, we performed a single-centre retrospective study of 36 adolescent LCH patients aged between 14 and 17 years at Peking Union Medical College Hospital.

Results: At the time of diagnosis, 10 patients were classified as unifocal single system LCH (27.8%), 2 patients had pulmonary single system LCH (5.6%), 5 patients had multifocal single system LCH with bone involvement (13.9%), and 19 patients had multisystem LCH (52.8%). The most prevalent involvement in multisystem patients was the pituitary gland (78.9%), followed by the bone (42.1%), lung (42.1%), and lymph nodes (42.1%). Eight (42.1%) patients had risk organ involvement. BRAF was detected in 50% of patients who underwent next generation sequencing, and BRAF was detected in one patient. Chemotherapies were the first line treatment in 24 patients. One patient died and thirteen patients relapsed during the follow-up. The estimated 5-year OS rate and EFS rate were 94.7% and 59.0%, respectively.

Conclusions: In this study, we report a large series of adolescent LCH patients. The clinical characteristics of adolescent LCH patients may be close to adult LCH. Compared with pediatric cases, adolescent LCH tends to have more pituitary lesions and pulmonary involvement, fewer skin and hematopoietic involvement, a higher frequency of BRAF deletion mutation, and a lower frequency of BRAF mutation.
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http://dx.doi.org/10.1186/s13023-022-02436-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288061PMC
July 2022

A novel heterozygous variant in PANX1 is associated with oocyte death and female infertility.

J Assist Reprod Genet 2022 Jul 14. Epub 2022 Jul 14.

JXHC Key Laboratory of Fertility Preservation, Jiangxi Maternal and Child Health Hospital, Nanchang, Jiangxi, 330006, People's Republic of China.

Purpose: Oocyte death is a severe clinical phenotype that causes female infertility and recurrent in vitro fertilization and intracytoplasmic sperm injection failure. We aimed to identify pathogenic variants in a female infertility patient with oocyte death phenotype.

Methods: Sanger sequencing was performed to screen PANX1 variants in the affected patient. Western blot analysis was used to check the effect of the variant on PANX1 glycosylation pattern in vitro.

Results: We identified a novel PANX1 variant (NM_015368.4 c.86G > A, (p. Arg29Gln)) associated with the phenotype of oocyte death in a non-consanguineous family. This variant displayed an autosomal dominant inheritance pattern with reduced penetrance. Western blot analysis confirmed that the missense mutation of PANX1 (c.86G > A) altered the glycosylation pattern in HeLa cells. Moreover, the mutation effects on the function of PANX1 were weaker than recently reported variants.

Conclusion: Our findings expand the inheritance pattern of PANX1 variants to an autosomal dominant mode with reduced penetrance and enrich the variational spectrum of PANX1. These results help us to better understand the genetic basis of female infertility with oocyte death.
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http://dx.doi.org/10.1007/s10815-022-02566-1DOI Listing
July 2022

Variation in placental microRNA expression associates with maternal family history of cardiovascular disease.

J Dev Orig Health Dis 2022 Jul 11:1-8. Epub 2022 Jul 11.

Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, USA.

In the United States, cardiovascular disease is the leading cause of death and the rate of maternal mortality remains among the highest of any industrialized nation. Maternal cardiometabolic health throughout gestation and postpartum is representative of placental health and physiology. Both proper placental functionality and placental microRNA expression are essential to successful pregnancy outcomes, and both are highly sensitive to genetic and environmental sources of variation. Placental pathologies, such as preeclampsia, are associated with maternal cardiovascular health but may also contribute to the developmental programming of chronic disease in offspring. However, the role of more subtle alterations to placental function and microRNA expression in this developmental programming remains poorly understood. We performed small RNA sequencing to investigate microRNA in placentae from the Rhode Island Child Health Study ( = 230). MicroRNA counts were modeled on maternal family history of cardiovascular disease using negative binomial generalized linear models. MicroRNAs were considered to be differentially expressed at a false discovery rate (FDR) less than 0.10. Parallel mRNA sequencing data and bioinformatic target prediction software were then used to identify potential mRNA targets of differentially expressed microRNAs. Nine differentially expressed microRNAs were identified (FDR < 0.1). Bioinformatic target prediction revealed 66 potential mRNA targets of these microRNAs, many of which are implicated in TGFβ signaling pathway but also in pathways involving cellular metabolism and immunomodulation. A robust association exists between familial cardiovascular disease and placental microRNA expression which may be implicated in both placental insufficiencies and the developmental programming of chronic disease.
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http://dx.doi.org/10.1017/S2040174422000319DOI Listing
July 2022

Mathematical Modelling and Hierarchical Encourage Particle Swarm Optimization Genetic Algorithm for Jet Pipe Servo Valve.

Comput Intell Neurosci 2022 1;2022:9155248. Epub 2022 Jul 1.

School of Electronic Engineering, Xi'an Shiyou University, No. 18, East Section of Electronic 2nd Road, Xi'an, Shannxi, China.

The jet pipe servo (JPS) valve is one key component, whose dynamic performance directly influences the aircraft's maneuverability. In this paper, a more accurate mathematical model and a novel multiobjective hierarchical encourage particle swarm optimization genetic algorithm (HEPGA) are proposed to improve the dynamic performance of the jet pipe servo valve. By optimizing the main structure parameters of the jet pipe servo valve, the adjustment and overshoot in the dynamic performance are reduced by 24.28% and 51.39%, respectively, compared with the prototype before optimization. To obtain a more accurate mathematical model, the computational fluid dynamics (CFD) is introduced to modify the analytical model considering the turbulent submerged free jet. Different from conventional numerical simulation, the dynamic mesh technique is used to analyze the flow field distribution by considering the force interaction of various parts of the jet pipe servo valve under actual working condition. Then, the HEPGA with better convergence is utilized because of the conflict of adjustment and overshoot. This proposed hybrid algorithm introduces the concept of staff welfare system to divide the population into elite individuals and excellent individuals of particle swarm optimization and general individuals of genetic algorithm. Meanwhile, the convergency performance of the HEPGA is evaluated through the Rosenbrock function by comparing with other particle swarm genetic hybrid methods. Subsequently, the experimental platform is constructed and the dynamic performance tests are conducted on the prototype after optimization. The experimental results verify the accuracy of the established mathematical model and the significant improvement of dynamic performance of the jet pipe servo valve.
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http://dx.doi.org/10.1155/2022/9155248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270145PMC
July 2022

Low expression of PRDM5 predicts poor prognosis of esophageal squamous cell carcinoma.

BMC Cancer 2022 Jul 7;22(1):745. Epub 2022 Jul 7.

Department of Radiation Oncology, Affiliated Tumor Hospital of Nantong University, Nantong Tumor Hospital, Nantong, Jiangsu, China.

Background: The role of the PRDM5 in esophageal squamous cell carcinoma (ESCC) has not been revealed. This study investigated the relationship between PRDM5 expression and survival outcome in esophageal squamous cell carcinoma and explored the mechanism in tumor development.

Methods: In present study, expression of PRDM5 mRNA in esophageal squamous cell carcinoma patients was conducted using the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database. The expression of PRDM5 was assessed by immunohistochemical staining. Kaplan-Meier curve and Cox regression analysis was performed to analyze the survival outcome and independent predictive factors. qRT-PCR and Methylation-specific PCR were performed to identify the mRNA level of PRDM5 and Methylation rate. Cibersort algorithm to analyze the relationship between PRDM5 expression and immune cell invasion. Western-blot was performed to confirm the expression of esophageal tumor tissues and adjacent tissues.

Results: The TCGA database and GEO database show that PRDM5 mRNA level in esophageal squamous cell carcinoma adjacent tissues was higher than that of cancer tissues, and ESCC patients with high expression of PRDM5 mRNA had better overall survival. Tissue microarray showed that the protein level of PRDM5 in the adjacent tissues of patients with ESCC was higher than that in cancer tissues, and the expression level of PRDM5 was significantly correlated with the grade of clinicopathological characteristics (P < 0.001). Patients with high expression of PRDM5 displayed a better OS and DFS. Cox regression analysis showed that PRDM5 was an independent risk factor and prognostic factor for ESCC patients (HR: 2.626, 95%CI: 1.824-3.781; P < 0.001). The protein level of PRDM5 matched with the transcriptional level, whereas the DNA methylation affected the transcriptional level. Cibersort showed that T cells CD4 memory resting, mast cells resting, eosinophils, M2 macrophages and mast cells activated were significantly positively correlated with PRDM5 expression (P < 0.05), while regulatory T cells, monocytes and dendritic cells negatively correlated with PRDM5 expression (P < 0.05).

Conclusion: PRDM5 can be used as a biomarker to predict the survival of ESCC patients. Furthermore, PRDM5 expression in ESCC cells may affect WNT/β-catenin signaling pathways, thus further affect the ESCC cell proliferation, migration, and invasion capacity.
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http://dx.doi.org/10.1186/s12885-022-09787-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9264607PMC
July 2022
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