Publications by authors named "Ji-Seok Kim"

39 Publications

Draft genome of Semisulcospira libertina, a species of freshwater snail.

Genomics Inform 2021 Sep 30;19(3):e32. Epub 2021 Sep 30.

Department of Orthopaedic Surgery, Gyeongsang National University Hospital, Jinju 52727, Korea.

Semisulcospira libertina, a species of freshwater snail, is widespread in East Asia. It is important as a food source. Additionally, it is a vector of clonorchiasis, paragonimiasis, metagonimiasis, and other parasites. Although S. libertina has ecological, commercial, and clinical importance, its whole-genome has not been reported yet. Here, we revealed the genome of S. libertina through de novo assembly. We assembled the whole-genome of S. libertina and determined its transcriptome for the first time using Illumina NovaSeq 6000 platform. According to the k-mer analysis, the genome size of S. libertina was estimated to be 3.04 Gb. Using RepeatMasker, a total of 53.68% of repeats were identified in the genome assembly. Genome data of S. libertina reported in this study will be useful for identification and conservation of S. libertina in East Asia.
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http://dx.doi.org/10.5808/gi.21039DOI Listing
September 2021

Two Types of Mouse Models for Sarcopenia Research: Senescence Acceleration and Genetic Modification Models.

J Bone Metab 2021 Aug 31;28(3):179-191. Epub 2021 Aug 31.

Department of Orthopaedic Surgery, Gyeongsang National University Hospital, Gyeongsang National University, Jinju, Korea.

Sarcopenia leads to loss of skeletal muscle mass, quality, and strength due to aging; it was recently given a disease code (International Classification of Diseases, Tenth Revision, Clinical Modification, M62.84). As a result, in recent years, sarcopenia-related research has increased. In addition, various studies seeking to prevent and treat sarcopenia by identifying the various mechanisms related to the reduction of skeletal muscle properties have been conducted. Previous studies have identified muscle synthesis and breakdown; investigating them has generated evidence for preventing and treating sarcopenia. Mouse models are still the most useful ones for determining mechanisms underlying sarcopenia through correlations and interventions involving specific genes and their phenotypes. Mouse models used to study sarcopenia often induce muscle atrophy by hindlimb unloading, denervation, or immobilization. Though it is less frequently used, the senescence-accelerated mouse can also be useful for sarcopenia research. Herein, we discuss cases where senescence-accelerated and genetically engineered mouse models were used in sarcopenia research and different perspectives to use them.
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http://dx.doi.org/10.11005/jbm.2021.28.3.179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441530PMC
August 2021

Validation of Dual Energy X-Ray Absorptiometry and Nuclear Magnetic Resonance in the Analysis of Body Composition in Mice.

J Bone Metab 2020 Nov 30;27(4):291-299. Epub 2020 Nov 30.

Department of Orthopaedic Surgery, Gyeongsang National University Hospital, Gyeongsang National University, Jinju, Korea.

Background: As an instrument for measuring body composition in experimental animals, dual energy X-ray absorptiometry (DXA) is ideal for accuracy, cost, and measurement efficiency. However, there is too little insight into the effectiveness of the various aspects of applying DXA to experimental animals. We investigated whether to compare and verify the precision and accuracy of DXA and nuclear magnetic resonance (NMR) animal body composition analyzers.

Methods: We used 30 Institution of Cancer Research mice in the study. First, in order to evaluate the reproducibility of DXA and NMR, we did repeated measurements by repositioning each mouse in anesthesia and euthanasia states. Subsequently, the accuracy of each device was evaluated by comparing the weight measured before the experiment, the weight of the tissue extracted from the mice after the experiment, and the measured DXA and NMR. In addition, when measuring the body composition of animals, we compared the time and the measurable body composition parameters and summarized the advantages and disadvantages of the 2 devices.

Results: Compared to NMR, DXA had the advantage of a fast measurement of bone composition and rapid image analysis. In addition, DXA showed a higher correlation (>95%) with fat mass, lean mass baseline than did NMR (>85%).

Conclusions: In conclusion, DXA was confirmed to have higher precision and measurement accuracy than did NMR. Therefore, DXA is an effective method for evaluating the body composition of experimental animals.
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http://dx.doi.org/10.11005/jbm.2020.27.4.291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746482PMC
November 2020

Study on the Pore Structure Characteristics of Ferronickel-Slag-Mixed Ternary-Blended Cement.

Materials (Basel) 2020 Oct 29;13(21). Epub 2020 Oct 29.

Department of Civil and Environmental Engineering, Hanyang University, Ansan 15588, Korea.

Pore structure development in Portland cement, fly ash, or/and ferronickel slag (FNS) was investigated using mercury intrusion porosimetry and X-ray CT tomography. The progress of hydration was observed using X-ray diffraction (XRD) analysis and compressive strength while durability of concrete was monitored by chloride penetration resistance and chloride profiles. Mercury intrusion porosimetry (MIP) results suggested that the blended cement had a higher porosity while lower critical pore size. The major reason to this increased porosity was the formation of meso and micro pores compared to ordinary Portland cement (OPC). In terms of chloride transport, replaced cement, especially ternary-blended cement had higher resistance to chloride transport and exhibited slightly lower development of compressive strength. X-ray CT tomography shows that the influence of pore structure of ternary-blended cement on the ionic transport was strongly related to the pore connectivity of cement matrix.
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http://dx.doi.org/10.3390/ma13214863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7662238PMC
October 2020

Microneedles with dual release pattern for improved immunological efficacy of Hepatitis B vaccine.

Int J Pharm 2020 Dec 16;591:119928. Epub 2020 Oct 16.

Department of BioNano Technology, Gachon BioNano Research Institute, Gachon University, Seongnam, Republic of Korea. Electronic address:

In this study, dissolving microneedles (DMNs) with dual-release pattern, capable of both bolus release and slow release, were prepared. These DMNs were used with a hepatitis B vaccine that requires multiple shots to achieve immunological efficacy comparable to that obtained when two separate shots are administered. Dissolving microneedles with HBsAg in PLA tips and CMC coating formulation together (HBsAg-PLA/CMC-DMNs) consist of polylactic acid (PLA) tips for slow release, a carboxy-methyl cellulose (CMC) coating formulation for bolus release, and a dissolving base of polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP) for dissolution in the skin. The in vitro release pattern of HBsAg from the CMC coating formulation and PLA tips was observed. Through an in vivo test, 1) the delivery efficiency of HBsAg-PLA/CMC-DMNs was observed, and 2) the immunological efficacy of this method was compared with the efficacy of two shots delivered by conventional intramuscular (IM) administration and two shots delivered by HBsAg-coated microneedle (CMNs) administration. HBsAg-PLA/CMC-DMNs punctured the skin successfully. The PVA/PVP base was completely dissolved within 10 min of insertion, resulting in the delivery of all microneedle tips into the skin. In the in vitro release experiment, all of the HBsAg in the CMC coating formulation was released within 20 min, and the HBsAg present in the PLA tips was gradually released over more than 55 days. The antibody titer of one shot of HBsAg-PLA/CMC-DMNs was the same as or higher than two shots delivered by conventional IM and CMN methods. DMNs with dual-release pattern can deliver two formulations simultaneously with a single shot, resulting in improved immunological efficacy of HBsAg that requires multiple doses. In addition, this dual-release MN system can be used for the delivery of other drugs that require multiple administrations.
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http://dx.doi.org/10.1016/j.ijpharm.2020.119928DOI Listing
December 2020

Effects of lifelong spontaneous exercise on the M1/M2 macrophage polarization ratio and gene expression in adipose tissue of super-aged mice.

Exp Gerontol 2020 11 12;141:111091. Epub 2020 Sep 12.

Department of Physical Education, Gyeongsang National University, Jinju, Republic of Korea. Electronic address:

In the adipose tissue (AT), an increase in the M1 macrophage (M1Ø)/M2 macrophage (M2Ø) polarization ratio can be a risk factor enhancing the inflammatory response during aging, as well as increasing the risk of chronic disease, thereby reducing lifespan, or at least reducing "healthy" lifespan. The purpose of this study was to analyze and compare the AT M1Ø/M2Ø polarization ratio at the final lifespan stage in aged and control animals performing lifelong spontaneous wheel running. Based on flow cytometric analysis, the AT ratio of macrophages revealed M2Ø polarization following lifelong spontaneous exercise (LSE) regardless of age. However, for Icam1 and Tnf, the qPCR analysis showed no difference in gene expressions in young mice; Arg1 expression was higher in Young-EXE (exercising) than in Young-CON (control) mice (p < .0001). In Old-EXE, Icam1 (p < .0001) and Tnf (p < .0001) expression were lower than in Old-CON; for Arg1, gene expression in Old-EXE was higher than in Old-CON (p < .0001). LSE prevents deterioration of physical fitness owing to aging, maintaining high M2Ø polarization levels in the AT. Additionally, LSE does not downregulate Icam1 and Tnf in the AT but appears to suppress the increased M1Ø polarization ratio attributed to aging by upregulating Arg1.
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http://dx.doi.org/10.1016/j.exger.2020.111091DOI Listing
November 2020

Genome-wide functional analysis of phosphatases in the pathogenic fungus Cryptococcus neoformans.

Nat Commun 2020 08 24;11(1):4212. Epub 2020 Aug 24.

Department of Life Science, College of Natural Science, Chung-Ang University, Seoul, 06974, Korea.

Phosphatases, together with kinases and transcription factors, are key components in cellular signalling networks. Here, we present a systematic functional analysis of the phosphatases in Cryptococcus neoformans, a fungal pathogen that causes life-threatening fungal meningoencephalitis. We analyse 230 signature-tagged mutant strains for 114 putative phosphatases under 30 distinct in vitro growth conditions, revealing at least one function for 60 of these proteins. Large-scale virulence and infectivity assays using insect and mouse models indicate roles in pathogenicity for 31 phosphatases involved in various processes such as thermotolerance, melanin and capsule production, stress responses, O-mannosylation, or retromer function. Notably, phosphatases Xpp1, Ssu72, Siw14, and Sit4 promote blood-brain barrier adhesion and crossing by C. neoformans. Together with our previous systematic studies of transcription factors and kinases, our results provide comprehensive insight into the pathobiological signalling circuitry of C. neoformans.
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http://dx.doi.org/10.1038/s41467-020-18028-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445287PMC
August 2020

Rodent Model of Muscular Atrophy for Sarcopenia Study.

J Bone Metab 2020 May 31;27(2):97-110. Epub 2020 May 31.

Department of Orthopaedic Surgery, Gyoengsang National University Hospital, Gyeongsang National University, Jinju, Korea.

The hallmark symptom of sarcopenia is the loss of muscle mass and strength without the loss of overall body weight. Sarcopenia patients are likely to have worse clinical outcomes and higher mortality than do healthy individuals. The sarcopenia population shows an annual increase of ~0.8% in the population after age 50, and the prevalence rate is rapidly increasing with the recent worldwide aging trend. Based on International Classification of Diseases, Tenth Revision, a global classification of disease published by the World Health Organization, issued the disease code (M62.84) given to sarcopenia in 2016. Therefore, it is expected that the study of sarcopenia will be further activated based on the classification of disease codes in the aging society. Several epidemiological studies and meta-analyses have looked at the correlation between the prevalence of sarcopenia and several environmental factors. In addition, studies using cell lines and rodents have been done to understand the biological mechanism of sarcopenia. Laboratory rodent models are widely applicable in sarcopenia studies because of the advantages of time savings, cost saving, and various analytical applications that could not be used for human subjects. The rodent models that can be applied to the sarcopenia research are diverse, but a simple and fast method that can cause atrophy or aging is preferred. Therefore, we will introduce various methods of inducing muscular atrophy in rodent models to be applied to the study of sarcopenia.
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http://dx.doi.org/10.11005/jbm.2020.27.2.97DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7297619PMC
May 2020

Characterization of Hepatitis B Surface Antigen Loaded Polylactic Acid-Based Microneedle and Its Dermal Safety Profile.

Pharmaceutics 2020 Jun 9;12(6). Epub 2020 Jun 9.

College of Pharmacy, Chungnam National University, Daejeon 34134, Korea.

A surge of interest in microneedle (MN) vaccines as a novel vaccination system has emerged. Before the clinical application of MN vaccine, an assessment of potential biological risks to skin and quality control of MN must be performed. Therefore, the present study aims to evaluate the physicochemical properties of MN and to evaluate the histological changes and inflammatory cell infiltrations after the application of MN with hepatitis B surface antigen (HBsAg). During in vitro and in vivo release testing, HBsAg MN released over 70% of HBsAg at 30 min. During the pyrogen test of HBsAg MN in rabbit, no rabbit showed an individual rise in temperature of 0.5 °C or more. MN with HBsAg produced the moderate immunization in mice. MN application did not alter the thickness of dermal and epidermal layers in mice. In addition, the topical applications of MN and MN for hepatitis B vaccine did not acutely induce the inflammation, allergic reaction, dermal toxicity and skin irritation. Thus, the MN system for the delivery of HBsAg could be the promising technology in the hepatitis B vaccination.
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http://dx.doi.org/10.3390/pharmaceutics12060531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7355901PMC
June 2020

Neural maturation enhanced by exercise-induced extracellular derivatives.

Sci Rep 2020 03 3;10(1):3893. Epub 2020 Mar 3.

Department of Physical Education, Gyeongsang National University, Jinju-daero, Jinju, 52828, Republic of Korea.

Physical activity has profound effects on neuronal progenitor cell growth, differentiation, and integration, but the mechanism for these effects is still ambiguous. Using a mouse model, we investigated the effects of two weeks of treadmill running on the dynamics of the size distribution and miRNA profiles of serum extracellular derivatives (EDs) using particle-sizing analysis and small RNA sequencing. We found that an increased average diameter of EDs in the running group compared with the sedentary group (p < 0.05), and 16 miRNAs were significantly altered (p < 0.05) in the running group. Furthermore, functional annotation analysis of differentially expressed miRNA-predicted target genes showed that many of these target genes are involved in the PI3K-Akt pathway. Exercise-induced serum EDs increased Neuro2A cell viability and Akt phosphorylation. We also found that expression levels of neuronal maturation markers such as Microtubule-Associated Protein 2 (MAP2ab) and Neuronal nuclei (NeuN) were increased (p < 0.05, respectively), and that inhibition of the PI3K-Akt pathway by LY294002 pre-treatment ameliorated their expression in Neuro2A cells. Finally, the administration of exercise-induced EDs for 3 days increased the Histone 3 phosphorylation and β-III tubulin expression in Ink/Arf null neural stem cells and progenitors (NSPCs) under each proliferation and differentiation condition. These results suggest that exercise-induced circulating EDs may mediate neuronal maturation during exercise.
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http://dx.doi.org/10.1038/s41598-020-60930-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054262PMC
March 2020

Associations Between Skeletal Muscle Mass, Grip Strength, and Physical and Cognitive Functions in Elderly Women: Effect of Exercise with Resistive Theraband.

J Exerc Nutrition Biochem 2019 Sep;23(3):50-55

Purpose: The purpose of this study was to identify the relationships between muscle mass, muscle strength, and physical and cognitive functions and to examine the effects of resistive Theraband® exercise on sarcopenia-associated variables in the older population.

Methods: A total of 28 elderly women (age: 69.90 ± 0.8 years) participated in this study, 15 of whom underwent elastic band exercise for 1 hour per day, twice per week for 8 weeks. The correlation analysis was conducted to identify the associations between body composition, skeletal muscle mass indices, grip strength, and physical and cognitive functions. All variables were assessed at baseline and post-exercise.

Results: Skeletal muscle mass was significantly associated with grip strength and physical function. Gait speed was positively correlated with grip strength and physical function, but not with cognitive function. Theraband® exercise significantly improved gait speed and physical function.

Conclusion: The present data suggest that skeletal muscle mass is highly correlated with grip strength and physical function. Eight weeks of resistive Theraband® exercise favorably affects sarcopenia by improving gait speed and mobility of elderly women.
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http://dx.doi.org/10.20463/jenb.2019.0023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823645PMC
September 2019

Skin immunization with third-generation hepatitis B surface antigen using microneedles.

Vaccine 2019 09 26;37(40):5954-5961. Epub 2019 Aug 26.

Department of BioNano Technology, Gachon BioNano Research Institute, Gachon University, Seongnam, Republic of Korea. Electronic address:

L-HBsAg is a third-generation hepatitis vaccine capable of inducing antibodies in non-responders and thus providing potentially therapeutic treatment. In this study, L-HBsAg was administered using microneedles (MN) without an adjuvant to induce intradermal (ID) immunization, and the efficacy of ID immunization was compared with that of intramuscular (IM) immunization that uses a conventional formulation with an adjuvant of aluminum hydroxide (L-HBsAg-AL-IM). The L-HBsAg was dip-coated onto 800-μm-long microneedles made of polylactic acid (PLA). Delivery efficiency and administration time were determined through in vitro experiments using porcine skin. The denaturation of the formulation against sterilization by gamma rays was observed. A storage test and a freeze-thaw cycle test of the microneedles with trehalose as a stabilizer (L-HBsAg-MN-Tre) were observed. An antibody titer of L-HBsAg-MN-Tre was compared with that of the conventional IM immunization of the L-HBsAg solution with aluminum hydroxide (L-HBsAg-AL-IM). The formulation containing L-HBsAg was located on the upper third of the microneedle tips. The formulation on the MN was dissolved and delivered within 30 min of insertion into porcine skin in vitro. Trehalose was selected as a stabilizer, and the stabilizing effect increased with the increase of trehalose content in the solidified formulation. L-HBsAg-MN with 15% of trehalose was stable for 7 days at 40 °C and showed increased stability compared to the conventional liquid formulations. L-HBsAg-MN-Tre showed improved stability during the freeze-thaw cycle. The antibody titer of L-HBsAg-MN-Tre at 28 days was higher than that of L-HBsAg-AL-IM. ID administration of L-HBsAg-MN-Tre showed better efficacy and improved thermal and freeze thaw stability compared to L-HBsAg-AL-IM. Therefore, L-HBsAg-MN-Tre administration showed the possibility of ID delivery of L-HBsAg without the use of an adjuvant for the efficacy, convenience, and safety of pediatric vaccination.
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http://dx.doi.org/10.1016/j.vaccine.2019.08.036DOI Listing
September 2019

Trends in exercise neuroscience: raising demand for brain fitness.

J Exerc Rehabil 2019 Apr 26;15(2):176-179. Epub 2019 Apr 26.

School of Exercise & Sport Science, College of Natural Sciences, University of Ulsan, Ulsan, Korea.

Physical exercise is increasingly recognized as an important component in the neuroscience related field. What is the targeting of exercise and what accounts for the exercise's benefits observed in neuroscience? Several types of exercise have been studied in various fields across physiological, psychological, and biochemical experiments of neuroscience. However, more clarity is needed to unveil optimal exercise conditions such as frequency, intensity, type, and time. In this review, we briefly highlight the positive effects of exercise on promoting brain function. Key areas relate to exercise neuroscience are as follow: structural level with synaptic plasticity and neurogenesis, functional level with behavioral development, and molecular level with possible mechanisms that involved in exercise-induced brain plasticity. Overall, we provide the importance of understanding the exercise neuroscience and highlight suggestions for future health research.
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http://dx.doi.org/10.12965/jer.1938046.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509468PMC
April 2019

Effects of resveratrol on laminar shear stress-induced mitochondrial biogenesis in human vascular endothelial cells.

J Exerc Nutrition Biochem 2019 Mar;23(1):7-12

Purpose: The purpose of the study was to determine the combined effects of resveratrol supplementation with high-flow LSS on mitochondrial biogenesis in human vascular endothelial cells.

Methods: Cultured human umbilical vein endothelial cells were treated with 20 μM of RSV. For the shear experiments, cells grown to a >90% confluence were exposed to physiological levels of LSS (5 to 20 dyne/cm2) for 12 to 36 hours using a cone and plate shear apparatus. Gene expressions were analyzed by western blotting.

Results: Depletion of mitochondrial integrity was directly associated with increase in endothelial activation/dysfunction. The expressions of mitochondrial biogenesis regulator genes, such as SIRT1, PGC-1α, and TFAM, and the mitochondrial contents were significantly increased after treatment with both resveratrol and high-flow LSS for 12 hours. However, supplementation of resveratrol to high-flow LSS for a prolonged duration had no synergistic effect on the levels of mitochondrial biogenesis regulator gene expressions and mitochondrial content compared to the LSS treatment alone.

Conclusion: The present study demonstrated that the supplementation of resveratrol to high-flow LSS has no synergistic effects on enhancing mitochondrial integrity in human vascular endothelial cells.
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http://dx.doi.org/10.20463/jenb.2019.0002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6477816PMC
March 2019

Neuropeptides Profile and Increased Innervation in Becker's Nevus.

Ann Dermatol 2019 Apr 28;31(2):154-163. Epub 2019 Feb 28.

Department of Dermatology, Dankook University College of Medicine, Cheonan, Korea.

Background: Melanocytes are derived from neural crest, and various pigmentary disorders may accompany abnormalities in nerve system or develop following dermatome, suggesting that melanocyte and pigmentation may be closely related to neural factors. There are reports of Becker's nevus (BN) showing linear and segmental configuration, suggesting the association of BN with nerve system. However, there are no studies regarding the expression of neuropeptides in BN.

Objective: We investigated the expression of neuropeptides and innervation in BN.

Methods: Polymerase chain reaction (PCR) array of 84 genes related to neuronal process was done. Among the genes with 10-fold or more increase in lesional, real-time PCR was performed for neuropeptide Y (NPY), galanin, neurotensin (NTS) and their receptors skin compared to normal skin. IHC stain was done to look for the expression of NPY, galanin, NTS and their receptors and the distribution of protein gene products (PGP) 9.5 immunoreactive nerve fibers.

Results: PCR array revealed that 16 out of 84 genes related to neuronal process were increased by 10-fold or more in lesional skin. In real-time PCR of NPY, galanin, NTS and their receptors, statistically significant increase of NPY1R (<0.05) and marginally significant increase of NPY2R, GAL2R, and NTS2R (<0.1) was verified in lesional skin. In immunohistochemistry, NPY, NPY1R NPY2R, and NTS2R were highly expressed in lesional skin and increased PGP 9.5 immunoreactive linear nerve fibers were found in the epidermis of BN.

Conclusion: NPY, galanin, NTS and their receptors and increased innervation may play a role in the pathogenesis of BN.
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http://dx.doi.org/10.5021/ad.2019.31.2.154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992669PMC
April 2019

A tearable dissolving microneedle system for shortening application time.

Expert Opin Drug Deliv 2019 03 22;16(3):199-206. Epub 2019 Feb 22.

a Department of BioNano Technology and Gachon BioNano Research Institute , Gachon University , Seongnam-si , South Korea.

Objectives: A tearable dissolving microneedle system (TD-MN system) was developed for shortening the time required to administer drugs into the skin through the dissolving microneedles.

Methods: TD-MN system consisted of an array of tearable dissolving microneedles (TD-MN) and micro-pillars. The microneedle tips had a female part to integrate with the micro-pillars. The micro-pillars exerted a vertical force to cause the separation of the tips from the base. The separation force exerted by six TD-MN arrays with different thicknesses of separation region was measured. The TD-MN system with trypan blue was inserted into porcine skin to observe the separation of the microneedle tips, and then calcein was added separately to observe drug diffusion into the skin.

Results: The thickness of the tearable region and the depth of the female part were a function of the concentration and volume of the molding solution. The separation force increased as the thickness of the tearable region increased. Nine tips were successfully separated from the base by applying a force of through the micro-pillars.

Conclusions: The TD-MN system could provide immediate administration of a drug, resulting in improved patient convenience as well as delivery of the correct drug dose.
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http://dx.doi.org/10.1080/17425247.2019.1583645DOI Listing
March 2019

Exercise training increases skeletal muscle strength independent of hypertrophy in older adults aged 75 years and older.

Geriatr Gerontol Int 2019 Mar 8;19(3):265-270. Epub 2019 Jan 8.

Department of Physical Education, Gyeongsang National University, Jinju, Korea.

Aim: We investigated whether exercise-mediated acquisition of muscle mass and strength would occur in a concurrent manner in older adults.

Methods: A total of 152 community-dwelling older adults (young-old aged 65-74 years, old-old aged >75 years) were allocated into either 8-week comprehensive exercise training or the control group. Participants (n = 136) completed all pre- and post-intervention testing visits (young-old n = 73, old-old n = 63). Older adults in exercise groups were subjected to a series of programmed elastic band and free exercises twice per week at three to five sets of 15-20 repetitions. Body composition, skeletal muscle mass, knee strength (extensors and flexors) and gait-related physical function were evaluated as main variables.

Results: As expected, muscular mass and knee strength (both extensors and flexors) were inversely correlated with age in the old-old group (all P < 0.001). However, knee extensor strength was the only lower limb component inversely correlated with age in the young-old group (P < 0.043). Knee extensor strength was significantly increased by exercise training in both the young-old and old-old groups (young old P < 0.042, old-old P < 0.011). Training-induced muscle hypertrophy was observed only in the young-old group (P < 0.025). the correlation of knee extensor strength against gait-related physical function was the greatest, followed by knee flexor strength and muscle mass.

Conclusions: The present results showed that age-associated strength decline of the knee extensor occurs earlier compared with the knee flexor during the aging process, and exercise training increases muscular strength without significant changes of muscle mass in older adults aged aged ≥75 years. Geriatr Gerontol Int 2019; 19: 265-270.
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http://dx.doi.org/10.1111/ggi.13597DOI Listing
March 2019

The effects of swimming training on arterial function, muscular strength, and cardiorespiratory capacity in postmenopausal women with stage 2 hypertension.

Menopause 2018 12;26(6):653-658

Department of Physical Education, Pusan National University, Busan, Korea.

Objective: Aging is associated with progressive decreases in arterial health as well as muscular strength and cardiorespiratory capacity. It is crucial to prevent or reduce the negative effects of aging on vasculature, muscular, and cardiorespiratory function by implementing appropriate lifestyle interventions, such as exercise training. We examined the effects of a swimming (SWM) regimen on arterial stiffness (pulse wave velocity [PWV]), blood pressure (BP), wave reflection (augmentation index [AIx]), muscle strength, and aerobic capacity in sedentary, postmenopausal women with stage 2 hypertension.

Methods: One hundred participants (∼74 years of age) were recruited from multiple general practice centers. Using a parallel experimental design, participants were randomly assigned to either SWM (n = 52) or nonexercising control group (n = 48) for 20 weeks. Participants in the SWM group trained 3 to 4 days/week. Participants' carotid to radial PWV, BP, AIx, muscular strength, and cardiorespiratory capacity were measured at baseline and after 20 weeks of their assigned intervention.

Results: There was a significant group × time interaction (P < 0.05) for carotid to radial PWV (Δ-1.2 m/s), AIx (Δ-4%), and systolic (Δ-9 mm Hg) and diastolic BP (Δ - 9 mm Hg), which significantly decreased (P < 0.05); and strength (Δ3 kg) and cardiorespiratory capacity (Δ4 mL/kg/min), which significantly increased (P < 0.05) following SWM compared to no changes in control.

Conclusions: SWM led to reductions in arterial stiffness, wave reflection, and BP while increasing strength and aerobic capacity in postmenopausal women with stage 2 hypertension. SWM may be an effective intervention in the prevention and treatment of age-related vascular complications and declines in muscle strength and cardiorespiratory capacity.
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http://dx.doi.org/10.1097/GME.0000000000001288DOI Listing
December 2018

Traumatic tibia shaft fractures caused by the impact of a golf ball: two case reports.

J Med Case Rep 2018 Aug 25;12(1):232. Epub 2018 Aug 25.

Department of Physical Education, Gyeongsang National University, Jinju, South Korea.

Background: As golf becomes increasingly popular, the number of injuries while playing golf also increases. We experienced two cases of traumatic tibia shaft fractures caused by the impact of a golf ball.

Case Presentation: A 48-year-old Korean man and a 43-year-old Korean man were diagnosed as having linear tibia shaft fractures on the right leg and left leg, respectively. Both of them were treated with closed intramedullary nails. Calculated impact power of the golf ball at the time of tibia fracture was 3372 and 5825 N, respectively. Radiologic and clinical complications such as nonunion and delayed union were not found up to the latest follow-up at 12 months postoperatively.

Conclusions: Players and watchers of golf must take precautionary measures before striking a golf ball. It is advisable that players watching a golfer hitting a golf ball should stand a long way behind or in front of the golfer striking the golf ball. The danger of such injury is on the rise because more and more people are enjoying golf nowadays. Warning players of such dangers is one preventive measure to avoid such injuries in the future.
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http://dx.doi.org/10.1186/s13256-018-1771-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6109352PMC
August 2018

Circulating metabolites of strawberry mediate reductions in vascular inflammation and endothelial dysfunction in db/db mice.

Int J Cardiol 2018 07 12;263:111-117. Epub 2018 Apr 12.

Department of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT 84112, USA. Electronic address:

Background: Cardiovascular disease is 2-4-fold more prevalent in patients with diabetes. Human studies support the cardiovascular benefits of strawberry consumption but the effects of strawberry on diabetic vasculature are unknown. We tested the hypothesis that dietary strawberry supplementation attenuates vascular inflammation and dysfunction in diabetic mice.

Methods: Seven-week-old diabetic db/db mice that consumed standard diet (db/db) or diet supplemented with 2.35% freeze-dried strawberry (db/db + SB) for ten weeks were compared to non-diabetic control mice (db/+). Indices of vascular inflammation and dysfunction were measured. Endothelial cells (ECs) were isolated from the vasculature to determine the influence of strawberry on them. The effect of metabolites of strawberry on endothelial inflammation was determined by incubating mouse aortic ECs (MAECs) with ±5% serum, obtained from strawberry fed mice (metabolites serum) or standard diet fed mice (control serum) ± 25 mM glucose and 100 μM palmitate.

Results: db/db mice exhibited an increased monocyte binding to vessel, elevated blood pressure, and reduced endothelial-dependent vasorelaxation compared with db/+ mice but each defect was attenuated in db/db + SB mice. The elevation of inflammatory molecules, NOX2 and inhibitor-κB kinase observed in ECs from db/db vs. db/+ mice was suppressed in db/db + SB mice. Glucose and palmitate increased endothelial inflammation in MAECs but were normalized by co-incubation with metabolites serum.

Conclusions: Dietary supplementation of strawberry attenuates indices of vascular inflammation and dysfunction in diabetic db/db mice. The effect of strawberry on vasculature is endothelial-dependent and possibly mediated through their circulating metabolites. Strawberry might complement conventional therapies to improve vascular complications in diabetics.
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http://dx.doi.org/10.1016/j.ijcard.2018.04.040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5951765PMC
July 2018

Clinico-Surgical Characteristics of Head and Neck Lipomas.

Dermatol Surg 2018 Jun;44(6):893-895

Department of Dermatology, College of Medicine, Dankook University, CheonAn, South Korea Department of Dermatology, Kyungpook National University School of Medicine, Daegu, South Korea Department of Dermatology, College of Medicine, Dankook University, CheonAn, South Korea.

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http://dx.doi.org/10.1097/DSS.0000000000001395DOI Listing
June 2018

Protective Effect of Topical Vitamin D3 against Photocarcinogenesis in a Murine Model.

Ann Dermatol 2016 Jun 25;28(3):304-13. Epub 2016 May 25.

Department of Dermatology, Dankook University College of Medicine, Cheonan, Korea.

Background: Although the incidence of non-melanoma skin cancer is increasing, there are no effective practical preventive measures other than avoiding sun exposure.

Objective: To elucidate the protective effect of topical application of biologically active vitamin D3 (calcitriol) on skin cancer development caused by exposure to ultraviolet (UV).

Methods: Groups of hairless mice were topically treated with either calcitriol or vehicle immediately after exposure to UVB and UVA three times weekly for the initial 20 weeks, and without UV exposure in the following 6 weeks. Tumor number was counted and biopsies were done for histopathologic analysis. The changes of cyclobutane pyrimidine dimer (CPD) were evaluated 1 hour and 11 hours after short term of UV exposure and application of calcitriol. For safety evaluation, blood test and body weights were evaluated at 23rd and 25th week.

Results: Total tumor count and number of tumors less than 3 mm in size tended to be fewer in calcitriol group, and tumors more than 3 mm in size showed significantly lower tumor formation rate in calcitriol group. Single application of calcitriol reduced CPD at 1 hour and 11 hours after UV exposure. Histopathologic analysis showed tumors with lower grade malignancy in calcitriol group which suggested a delay in tumor progression. However, serum levels of calcium and phosphate in calcitriol group were above normal range, and weight loss was found.

Conclusion: Topical calcitriol may suppress the formation and progression of UV-induced non-melanoma skin cancer by enhancing the repair mechanism of UV damage.
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http://dx.doi.org/10.5021/ad.2016.28.3.304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4884706PMC
June 2016

Treatment of Melasma with the Photoacoustic Twin Pulse Mode of Low-Fluence 1,064 nm Q-Switched Nd:YAG Laser.

Ann Dermatol 2016 Jun 25;28(3):290-6. Epub 2016 May 25.

Department of Dermatology, Dankook University College of Medicine, Cheonan, Korea.

Background: Low-fluence 1,064 nm Q-switched Nd:YAG laser has been widely used for the treatment of melasma. Although new Q-switched Nd:YAG lasers with photoacoustic twin pulse (PTP) mode have been recently developed for high-efficiency, there is limited information available for the new technique.

Objective: This study was designed to investigate the efficacy and adverse effects after few sessions of repeated low fluence 1,064 nm Q-switched Nd:YAG laser treatment with PTP mode in Asian women with melasma.

Methods: Twenty-two Korean women were treated with a total of five sessions of low-fluence PTP mode Nd:YAG laser treatment (Pastelle®) at 2 weeks interval. Responses to treatments were evaluated by using Melasma Area and Severity Index (MASI) scoring, colorimeter measurement, and the investigators' and patients' overall assessments. Adverse events were recorded at each visit.

Results: Investigators' and patients' overall assessment showed that 'significantly improved' was assessed by 13 (59.1%) and 19 of 22 patients (86.4%), respectively. MASI scores were significantly reduced by 20.4%. The lightness, measured by using a colorimeter, was significantly increased by 1.3 point. Notable adverse events were not observed.

Conclusion: After 5 sessions of laser therapy alone, about 60% of the subjects showed significant improvement. Few sessions of repeated laser toning treatment using the PTP mode is a safe and effective way to treat facial melasma.
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http://dx.doi.org/10.5021/ad.2016.28.3.290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4884704PMC
June 2016

Extranodal Natural Killer/T-Cell Lymphoma, Nasal Type Involving Skin Masquerading as Eczema.

Ann Dermatol 2016 Apr 31;28(2):275-7. Epub 2016 Mar 31.

Department of Dermatology, Dankook University Medical College, Cheonan, Korea.

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http://dx.doi.org/10.5021/ad.2016.28.2.275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4828405PMC
April 2016

Effect of Interleukin-10 and Laminar Shear Stress on Endothelial Nitric Oxide Synthase and Nitric Oxide in African American Human Umbilical Vein Endothelial Cells.

Ethn Dis 2015 Nov 5;25(4):413-8. Epub 2015 Nov 5.

2. Department of Kinesiology, Temple University ; 4.Cardiovascular Research Center, Temple University School of Medicine.

Background: African Americans have a predisposition to heightened systemic inflammation and a high prevalence of hypertension.

Objective: The purpose of this study was to evaluate the influence of interleukin-10 (IL-10) and laminar shear stress (LSS) on African American endothelial cells by measuring total endothelial nitric oxide synthase (eNOS) protein expression and its phosphorylated form (p-eNOS) at Serine 1177, and nitric oxide (NO) levels, in response to IL-10 incubation and high physiological levels of LSS, used as an in vitro mimetic for aerobic exercise training (AEXT).

Design: Human umbilical vein endothelial cells (HUVEC) from an African American donor were cultured. The experimental conditions included Static, Static with IL-10 Incubation, LSS at 20 dynes/cm², and LSS at 20 dynes/cm² with IL-10 Incubation. Western blotting was used to measure eNOS and p-eNOS protein expression in the cells. A modified Griess assay was used to measure NO metabolites in the cell culture media.

Results: There were significant increases in p-eNOS, eNOS, and NO in the LSS at 20 dynes/cm² and LSS at 20 dynes/cm² with IL-10 Incubation experimental conditions when compared to the Static experimental condition. There were no other statistically significant differences demonstrating that IL-10 did not have an additive effect on eNOS activity in our study.

Conclusion: The significant increases in p-eNOS, eNOS, and NO as a result of LSS in African American HUVECs suggest that AEXT may be a viable, nonpharmacologic method to improve vascular inflammation status and vasodilation, and thereby contribute to hypertension reduction in the African American population.
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http://dx.doi.org/10.18865/ed.25.4.413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671427PMC
November 2015

Concurrent Drug-Induced Linear Immunoglobulin A Dermatosis and Immunoglobulin A Nephropathy.

Ann Dermatol 2015 Jun 29;27(3):315-8. Epub 2015 May 29.

Department of Dermatology, Dankook University Medical College, Cheonan, Korea.

Diseases associated with immunoglobulin A (IgA) antibody include linear IgA dermatosis, IgA nephropathy, Celiac disease, Henoch-Schönlein purpura, etc. Although usually idiopathic, IgA antibody is occasionally induced by drugs (e.g., vancomycin, carbamazepine, ceftriaxone, and cyclosporine), malignancies, infections, and other causes. So far, only a few cases of IgA bullous dermatosis coexisting with IgA nephropathy have been reported. A 64-year-old female receiving intravenous ceftriaxone and metronidazole for liver abscess had purpuric macules and papules on her extremities. One week later, she had generalized edema and skin rash with bullae and was diagnosed with concurrent linear IgA dermatosis and IgA nephropathy. After steroid treatment, the skin lesion subsided within two weeks, and kidney function slowly returned to normal. As both diseases occurred after a common possible cause, we predict their pathogeneses are associated.
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http://dx.doi.org/10.5021/ad.2015.27.3.315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466286PMC
June 2015

Shear stress-induced mitochondrial biogenesis decreases the release of microparticles from endothelial cells.

Am J Physiol Heart Circ Physiol 2015 Aug 29;309(3):H425-33. Epub 2015 May 29.

Department of Kinesiology, Temple University, Philadelphia, Pennsylvania; Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania; and

The concept of enhancing structural integrity of mitochondria has emerged as a novel therapeutic option for cardiovascular disease. Flow-induced increase in laminar shear stress is a potent physiological stimulant associated with exercise, which exerts atheroprotective effects in the vasculature. However, the effect of laminar shear stress on mitochondrial remodeling within the vascular endothelium and its related functional consequences remain largely unknown. Using in vitro and in vivo complementary studies, here, we report that aerobic exercise alleviates the release of endothelial microparticles in prehypertensive individuals and that these salutary effects are, in part, mediated by shear stress-induced mitochondrial biogenesis. Circulating levels of total (CD31(+)/CD42a(-)) and activated (CD62E(+)) microparticles released by endothelial cells were significantly decreased (∼40% for both) after a 6-mo supervised aerobic exercise training program in individuals with prehypertension. In cultured human endothelial cells, laminar shear stress reduced the release of endothelial microparticles, which was accompanied by an increase in mitochondrial biogenesis through a sirtuin 1 (SIRT1)-dependent mechanism. Resveratrol, a SIRT1 activator, treatment showed similar effects. SIRT1 knockdown using small-interfering RNA completely abolished the protective effect of shear stress. Disruption of mitochondrial integrity by either antimycin A or peroxisome proliferator-activated receptor-γ coactivator-1α small-interfering RNA significantly increased the number of total, and activated, released endothelial microparticles, and shear stress restored these back to basal levels. Collectively, these data demonstrate a critical role of endothelial mitochondrial integrity in preserving endothelial homeostasis. Moreover, prolonged laminar shear stress, which is systemically elevated during aerobic exercise in the vessel wall, mitigates endothelial dysfunction by promoting mitochondrial biogenesis.
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http://dx.doi.org/10.1152/ajpheart.00438.2014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525091PMC
August 2015

Bioinformatic identification of connective tissue growth factor as an osteogenic protein within skeletal muscle.

Physiol Rep 2014 Dec 24;2(12). Epub 2014 Dec 24.

Cardiovascular Genomics Laboratory, Department of Kinesiology, College of Public Health, Temple University, Philadelphia, Pennsylvania Cardiovascular Research Center, School of Medicine, Temple UniversityPhiladelphia, Pennsylvania.

Aging is associated with increasing incidence of osteoporosis; a skeletal disorder characterized by compromised bone strength that may predispose patients to an increased risk of fracture. It is imperative to identify novel ways in which to attenuate such declines in the functional properties of bone. The purpose of this study was to identify, through in silico, in vitro, and in vivo approaches, a protein secreted from skeletal muscle that is putatively involved in bone formation. We performed a functional annotation bioinformatic analysis of human skeletal muscle-derived secretomes (n = 319) using DAVID software. Cross-referencing was conducted using OMIM, Unigene, UniProt, GEO, and CGAP databases. Signal peptides and transmembrane residues were analyzed using SignalP and TMHMM software. To further investigate functionality of the identified protein, L6 and C2C12 myotubes were grown for in vitro analysis. C2C12 myotubes were subjected to 16 h of glucose deprivation (GD) prior to analysis. In vivo experiments included analysis of 6-week calorie restricted (CR) rat muscle samples. Bioinformatic analysis yielded 15 genes of interest. GEO dataset analysis identified BMP5, COL1A2, CTGF, MGP, MMP2, and SPARC as potential targets for further processing. Following TMHMM and SignalP processing, CTGF was chosen as a candidate gene. CTGF expression level was increased during L6 myoblast differentiation (P < 0.01). C2C12 myotubes showed no change in response to GD. Rat soleus muscle samples exhibited an increase in CTGF expression (n = 16) in response to CR (35%) (P < 0.05). CTGF was identified as a skeletal muscle expressed protein through bioinformatic analysis of skeletal muscle-derived secretomes and in vitro/in vivo analysis. Future study is needed to determine the role of muscle-derived CTGF in bone formation and remodeling processes.
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http://dx.doi.org/10.14814/phy2.12255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332228PMC
December 2014

Intralesional 3% sodium tetradecyl sulfate for treatment of cutaneous Kaposi's sarcoma.

Yonsei Med J 2015 Jan;56(1):307-8

Department of Dermatology, College of Medicine, Dankook University, Cheonan, Korea.

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http://dx.doi.org/10.3349/ymj.2015.56.1.307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4276773PMC
January 2015
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