Publications by authors named "Ji-Hyun Bae"

43 Publications

Gender disparities in childhood obesity and household food insecurity.

Nutrition 2021 Feb 9;87-88:111190. Epub 2021 Feb 9.

Department of Food Science and Nutrition, Keimyung University, Daegu, Korea. Electronic address:

Objectives: Childhood obesity is a critical health issue. The etiology of childhood obesity is multifactorial, with age, gender, race/ethnicity, and socioeconomic status interacting to affect risk. Food insecurity is known to be associated with risk of childhood obesity, but the body of evidence regarding Koreans is lacking. This study investigated the association between childhood obesity and household food insecurity in Koreans. Other lifestyle and nutritional factors associated with obesity were also examined.

Methods: Using data from the Korean National Health and Nutrition Examination Survey, a cross-sectional study was conducted with 1527 boys and 1366 girls. A comparison of general characteristics and nutritional intake between the groups was made using Student's t tests, χ tests, and general linear models. The association between childhood obesity and food insecurity was estimated with logistic regression models, and presented with odds ratios and 95% confidence intervals either with or without covariates.

Results: Boys who were obese dined out less frequently and engaged less in regular exercise, but no differences in nutrition intake were observed between children who were and were not obese. Girls who were obese were less likely to have a caregiver and consumed a higher percentage of energy from protein. Boys experiencing household food insecurity were less likely to be obese (adjusted odds ratio, 0.25; 95% confidence interval, 0.06-0.99), but girls with food insecurity were at three times higher risk of obesity (adjusted odds ratio, 3.00; 95% confidence interval, 1.23-7.31).

Conclusions: Differential lifestyle factors are associated with obesity phenotypes in boys and girls. Food insecurity also showed a contrasting association with obesity risk by gender in young Koreans.
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http://dx.doi.org/10.1016/j.nut.2021.111190DOI Listing
February 2021

The effect of diet, exercise, and lifestyle intervention on childhood obesity: A network meta-analysis.

Clin Nutr 2020 Nov 11. Epub 2020 Nov 11.

Home Economics Education, Kyungpook National University, Daegu, South Korea. Electronic address:

Background & Aims: Trials investigating the efficacy of different interventions for overweight children are limited and controversial. Therefore, the aim of this study is to perform a network meta-analysis on the efficacy of various interventions for children with obesity (an average age of 6-12 years old).

Methods: We obtained the data of trials reporting pre-post obesity relevant outcomes (e.g. BMI, BMI z-score, percent body fat, or percent overweight) from the Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE (Ovid), PubMed, and Web of Science databases (completed before February 25, 2019) and included at least one pair of direct comparison groups. The mean difference of outcomes and their associated 95% CI were used to determine the efficacy. The P-score was calculated to illustrate the rank probability of various treatments for different outcomes using a network meta-analysis. Our meta-analysis included 24 studies that evaluated the interventions for childhood obesity.

Results: All 24 trials had no high risk of bias. Interventions such as exercise without parents (E w/o P); diet with parents (D w/P); and diet, exercise, and lifestyle with parents (D+E+L w/P) were significantly effective for children with obesity when compared with no intervention.

Conclusions: E w/o P exhibited the highest P-score, with the D w/P and D+E+L w/P ranks having P-scores of 0.7486 and 0.5464, respectively. Moreover, the results indicate that E w/o P, D w/P, and D+E+L w/P were significantly effective treatments for children with obesity when compared with no intervention.
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http://dx.doi.org/10.1016/j.clnu.2020.11.006DOI Listing
November 2020

Ledeb. Ameliorates Hyperglycemia and Hepatic Steatosis in Ovariectomized Rats Fed a High-Fat Diet.

Nutrients 2020 Jun 1;12(6). Epub 2020 Jun 1.

Division of Applied Food System, Major of Food and Nutrition, Seoul Women's University, Seoul 01797, Korea.

Estrogen deficiency is associated with obesity, dyslipidemia, and increased insulin resistance in postmenopausal women. An efficient therapeutic agent prevents or improves postmenopausal conditions induced by estrogen deficiency. Here, we investigated the effects of aqueous Ledeb. extract on glucose and lipid metabolism in ovariectomized rats fed a high-fat diet (HFD). Female Sprague-Dawley rats were sham-operated or ovariectomized, and 3 weeks later were assigned to the following groups: sham-operated + HFD (S); ovariectomized + HFD (OVX); and ovariectomized + HFD with 0.5% aqueous extract (OVX + 0.5A) groups. Ovariectomy significantly increased body weight and dietary intake relative to the S group. However, treatment did not significantly affect weight gain or dietary intake. Blood triacylglycerol, total cholesterol, and low-density lipoprotein cholesterol levels tended to decrease in the -supplemented group. Blood glucose levels were significantly lower in the OVX + 0.5A group than those in the OVX group. Blood adiponectin and insulin concentrations increased significantly after treatment in the ovariectomized group. supplementation tended to decrease liver weights and prevented lipid accumulation. These effects correlated with reduced hepatic expression of lipogenesis-related genes (fatty acid synthase, acetyl-coenzyme A carboxylase alpha, and 3-hydroxy-3-methylglutaryl-coenzyme A reductase). Therefore, may improve metabolic disorders in ovariectomized rats.
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http://dx.doi.org/10.3390/nu12061631DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7352636PMC
June 2020

Efficient and specific generation of knockout mice using CRISPR/Cas9 system.

Biochem Biophys Rep 2020 Jul 1;22:100752. Epub 2020 Apr 1.

Graduate School of International Agricultural Technology, Designed Animal and Transplantation Research Institute, Seoul National University, 1447 Pyeongchang-Ro, Daewha, Pyeongchang, Kangwon, 25354, Republic of Korea.

The CRISPR/Cas9 (SpCas9) system is now widely utilized to generate genome engineered mice; however, some studies raised issues related to off-target mutations with this system. Herein, we utilized the Cas9 (CjCas9) system to generate knockout mice. We designed sgRNAs targeting mouse or and microinjected into zygotes along with CjCas9 mRNA. We obtained newborn mice from the microinjected embryos and confirmed that 50% (Tyr) and 38.5% (Foxn1) of the newborn mice have biallelic mutation on the intended target sequences, indicating efficient genome targeting by CjCas9. In addition, we analyzed off-target mutations in founder mutant mice by targeted deep sequencing and whole genome sequencing. Both analyses revealed no off-target mutations at potential off-target sites predicted and no unexpected random mutations in analyzed founder animals. In conclusion, the CjCas9 system can be utilized to generate genome edited mice in a precise manner.
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http://dx.doi.org/10.1016/j.bbrep.2020.100752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118303PMC
July 2020

Effect of Paper-Based Cognitive Training in Early Stage of Alzheimer's Dementia.

Dement Neurocogn Disord 2019 Jun 3;18(2):62-68. Epub 2019 Jul 3.

Department of Neurology, Veterans Medical Research Institute, Veterans Health Service Medical Center, Seoul, Korea.

Background And Purpose: Cognitive training refers to a series of standardized tasks with inherent challenges that target specific cognitive domains. Positive outcome of cognitive training in persons with Alzheimer's disease has been reported. In this study, the objective was to design sets of cognitive training program, "Gipum-seo" which is combined cognitive training, consists of different levels of difficulty using predesigned paper-and-pencil exercises. Also, to evaluate the effects of the cognitive training on patients' with early stage of Alzheimer's disease.

Methods: The subjects for this study were forty participants who were diagnosed with early stage of Alzheimer's dementia. To test the efficacy of paper-based cognitive training programs to cognition, all patients were randomly grouped to either an intervention group (=20) or a control group (=20). The intervention group regularly received 24 sessions of paper-based cognitive training over a 12-week period. Neuropsychological examinations were conducted before and after this training period.

Results: After the 12 weeks, the intervention group showed a significant change in Korean version of the Mini-Mental State Examination (25.90±3.8), compared to the control group (23.7±2.8) (=0.042). The training group also showed a significant improvement in language, attention and executive function, as compared with controls.

Conclusions: Paper-based cognitive training might have beneficial effects on the general cognitive functions in the early stage of Alzheimer's dementia.
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http://dx.doi.org/10.12779/dnd.2019.18.2.62DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609531PMC
June 2019

Treatment of non-odontogenic orofacial pain using botulinum toxin-A: a retrospective case series study.

Maxillofac Plast Reconstr Surg 2018 Dec 6;40(1):21. Epub 2018 Aug 6.

3Department of Conservative Dentistry, Section of Dentistry, Seoul National University Bundang Hospital, 300 Gumi-dong, Bundang-gu, Seongnam city, Gyunggi-do South Korea.

Background: The purpose of this study was to evaluate the clinical outcomes of treatment of non-odontogenic atypical orofacial pain using botulinum toxin-A.

Methods: This study involved seven patients (seven females, mean age 65.1 years) who had non-odontogenic orofacial pain (neuropathic pain and atypical orofacial pain) and visited the Seoul National University Bundang Hospital between 2015 and 2017. All medication therapies were preceded by botulinum toxin-A injections, followed by injections in the insignificant effects of medication therapies. Five of the seven patients received intraoral injections in the gingival vestibule or mucosa, while the remaining two received extraoral injections in the masseter and temporal muscle areas.

Results: In five of the seven patients, pain after botulinum toxin-A injection was significantly reduced. Most of the patients who underwent surgery for dental implantation or facial nerve reconstruction recovered after injections. However, the pain did not disappear in two patients who reported experiencing persistent pain without any cause.

Conclusions: The use of botulinum toxin-A for the treatment of non-odontogenic neuropathic orofacial pain is clinically useful. It is more effective to administer botulinum toxin-A in combination with other medications and physical therapy to improve pain.
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http://dx.doi.org/10.1186/s40902-018-0159-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6093826PMC
December 2018

Zinc transporter SLC39A11 polymorphisms are associated with chronic gastritis in the Korean population: the possible effect on spicy food intake.

Nutr Res 2018 09 22;57:78-85. Epub 2018 Apr 22.

Department of Food Science and Nutrition, College of Natural Sciences, Keimyung University, Daegu, Republic of Korea. Electronic address:

Herein, we hypothesized that ZIP11 variants would be important risk factors for chronic gastritis and that there would be an interaction effect of the relationship between their variants and spicy food intake on the development of chronic gastritis. Participants in this cross-sectional study (n = 3882 + 252) were recruited from a cohort of the Korean Genome and Epidemiology Study in 2001. Age, sex, education, smoking and drinking status, exercise, stress, and income level of all participants were determined by a questionnaire. Demographic and anthropometric data were collected. Fasting blood samples were collected to determine the serum levels of glucose, insulin, total bilirubin, total cholesterol, high-density lipoprotein cholesterol, and triglycerides. The presence of chronic gastritis was defined as a confirmed diagnosis by a physician. Food consumption was determined using a semiquantitative food frequency questionnaire. We found 8 different single nucleotide polymorphisms (SNPs) that are significantly different between subjects without gastritis and those with gastritis. Of these 8 SNPs, 3 SNP (rs17183225 [C/T], rs17780814 [A/C], and rs17780820 [A/G]) are closely located in the intronic region of zinc transporter SLC39A11, commonly known as ZIP11, and show linkage disequilibrium (D' = 1.0). We also found that participants with (TCA + TCG) haplotype of ZIP11 at high levels of dietary intake of spicy foods show a significantly increasing tendency in the odds of having chronic gastritis when compared with those with CAA haplotype (odds ratio, 2.620; 95% confidence interval, 1.207-5.689). The data indicate positive associations between higher meal frequency and lower spicy food preference and gastritis. In conclusion, we found that zinc transporter gene ZIP11 is associated with chronic gastritis in the Korean population and it may interact with spicy food, which suggests ZIP11 as a therapeutic target for precision nutrition.
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http://dx.doi.org/10.1016/j.nutres.2018.04.014DOI Listing
September 2018

Presence of Dental Caries Is Associated with Food Insecurity and Frequency of Breakfast Consumption in Korean Children and Adolescents.

Prev Nutr Food Sci 2018 Jun 30;23(2):94-101. Epub 2018 Jun 30.

Department of Food Science and Nutrition, Keimyung University, Daegu 41566, Korea.

Dental caries remains one of the most common chronic diseases affecting children worldwide with a multi-factorial etiology. The objective of the study was to evaluate the association between socioeconomic status (SES), dietary intake, food insecurity (FI), and dental caries in Korean children and adolescents. The study utilized data from the 2-year Korean National Health and Nutrition Examination Survey (KNHANES) conducted with 1,559 Korean boys and 1,391 girls aged 2 to 18 years from 2012 to 2013. Fathers' education (=0.017), mothers' education (<0.001), and household income (=0.049) were all significantly associated with dental caries among Korean boys. As for dietary practices, both eating breakfast (<0.001) and frequency of eating out (<0.001) were strongly associated with dental caries (<0.001). Three models of FI were used and no differences were found regarding genders. In model 3, both food insecure male [odds ratio (OR)=1.682, 95% confidence interval (CI): 0.999~2.832] and female (OR=1.900, 95% CI: 1.094~3.299) subjects had higher odds of developing dental caries than food secure subjects after adjusting the confounding factors. The present study showed a strong association between FI mediated by SES and dental caries. Nutrition education programs targeting low-socioeconomic families are necessary as a tool to prevent dental caries in Korea.
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http://dx.doi.org/10.3746/pnf.2018.23.2.94DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6047872PMC
June 2018

Multiple sgRNAs with overlapping sequences enhance CRISPR/Cas9-mediated knock-in efficiency.

Exp Mol Med 2018 04 6;50(4):1-9. Epub 2018 Apr 6.

Graduate School of International Agricultural Technology, Seoul National University, 1447 Pyeongchang-Ro, Daewha, Pyeongchang, Kangwon, 25354, Korea.

The CRISPR/Cas9 system is widely applied in genome engineering due to its simplicity and versatility. Although this has revolutionized genome-editing technology, knockin animal generation via homology directed repair (HDR) is not as efficient as nonhomologous end-joining DNA-repair-dependent knockout. Although its double-strand break activity may vary, Cas9 derived from Streptococcus pyogenens allows robust design of single-guide RNAs (sgRNAs) within the target sequence; However, prescreening for different sgRNA activities delays the process of transgenic animal generation. To overcome this limitation, multiple sets of different sgRNAs were examined for their knockin efficiency. We discovered profound advantages associated with single-stranded oligo-donor-mediated HDR processes using overlapping sgRNAs (sharing at least five base pairs of the target sites) as compared with using non-overlapping sgRNAs for knock-in mouse generation. Studies utilizing cell lines revealed shorter sequence deletions near target mutations using overlapping sgRNAs as compared with those observed using non-overlapping sgRNAs, which may favor the HDR process. Using this simple method, we successfully generated several transgenic mouse lines harboring loxP insertions or single-nucleotide substitutions with a highly efficiency of 18-38%. Our results demonstrate a simple and efficient method for generating transgenic animals harboring foreign-sequence knockins or short-nucleotide substitutions by the use of overlapping sgRNAs.
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http://dx.doi.org/10.1038/s12276-018-0037-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5938013PMC
April 2018

The Melatonin Signaling Pathway in a Long-Term Memory In Vitro Study.

Molecules 2018 Mar 23;23(4). Epub 2018 Mar 23.

Department of Medical Genetics, Hanvit Institutute for Medical Genetics, School of Medicine, Keimyung University, Daegu 42601, Korea.

The activation of cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) via phosphorylation in the hippocampus is an important signaling mechanism for enhancing memory processing. Although melatonin is known to increase CREB expression in various animal models, the signaling mechanism between melatonin and CREB has been unknown in vitro. Thus, we confirmed the signaling pathway between the melatonin receptor 1 (MT1) and CREB using melatonin in HT-22 cells. Melatonin increased MT1 and gradually induced signals associated with long-term memory processing through phosphorylation of Raf, ERK, p90RSK, CREB, and BDNF expression. We also confirmed that the calcium, JNK, and AKT pathways were not involved in this signaling pathway by melatonin in HT-22 cells. Furthermore, we investigated whether melatonin regulated the expressions of CREB-BDNF associated with long-term memory processing in aged HT-22 cells. In conclusion, melatonin mediated the MT1-ERK-p90RSK-CREB-BDNF signaling pathway in the in vitro long-term memory processing model and increased the levels of p-CREB and BDNF expression in melatonin-treated cells compared to untreated HT-22 cells in the cellular aged state. Therefore, this paper suggests that melatonin induces CREB signaling pathways associated with long-term memory processing in vitro.
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http://dx.doi.org/10.3390/molecules23040737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017053PMC
March 2018

Systematic review and meta-analysis of omega-3-fatty acids in elderly patients with depression.

Nutr Res 2018 02 8;50:1-9. Epub 2017 Dec 8.

Department of Nursing, Keimyung University, Daegu, South Korea. Electronic address:

One of the typical symptoms of a psychological crisis is depression, an increasing concern in the elderly population. Although omega-3-polyunsaturated fatty acids (PUFAs) are reported to be promising nutrients for treating depression, currently, there are no systematic reviews or meta-analyses of randomized control trials that provide critical evidence regarding the potential benefits of omega-3 fatty acids in elderly patients with depression. This analysis was conducted to provide evidence for the clinical application of omega-3 fatty acids in the treatment of depressive symptoms of elderly subjects older than 65 years. Seven databases were searched from their inception date until September 2016. Following this search, 6 studies were selected, which included 4605 patients (mean age, 76.97 years; male-female ratio=3752:853; mean dose of omega 3 intake, 1.3 g/d). These results were divided into 2 categories: well-being mental health group and depressive group. In the well-being mental health group, the Hedges g was 0.12 (95% confidence interval, -0.05 to 0.29), which indicated no significant effect of n-3 PUFA supplementation on depressed mood compared with placebo. In the depressive group, the pooled Hedges g was -0.94 (95% CI, -1.37 to -0.50]) for the random-effects model, which indicated a large effect of n-3 PUFA supplementation on those with depressed mood compared with placebo. Although this review shows that omega-3 fatty acids are effective in the treatment of elderly depressed patients, the benefits of omega-3 fatty acid supplementation were significant only in the elderly patients with mild to moderate depression.
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http://dx.doi.org/10.1016/j.nutres.2017.10.013DOI Listing
February 2018

Neuronal Nitric Oxide Synthase Is a Novel Biomarker for the Interstitial Cells of Cajal in Stress-Induced Diarrhea-Dominant Irritable Bowel Syndrome.

Dig Dis Sci 2018 03 25;63(3):619-627. Epub 2018 Jan 25.

Graduate School of International Agricultural Technology, Seoul National University, 1447 Pyeongchang-Ro, Daewha, Pyeongchang, Gangwon, 25354, Republic of Korea.

Background: Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder involving changes in normal bowel movements. The pathophysiology of IBS is not clearly understood owing to the lack of identifiable pathological abnormalities and reliable biomarkers.

Aim: The aim of this study was to discover the novel and reliable biomarker for IBS.

Method: In this study, neonatal maternal separation (NMS) stress model was used for the IBS mouse model. Further assessment was conducted with whole gastrointestinal transit test, quantitative RT-PCR, histological examination, and western blot.

Results: Male pups developed symptoms similar to those of human IBS with diarrhea (IBS-D), such as low-grade inflammation, stool irregularity, and increased bowel motility. NMS stress influenced to the interstitial cells of Cajal (ICC) and induced altered bowel motility, resulting in IBS-D-like symptoms. In addition, we found neuronal nitric oxide synthase (nNOS) to be a novel biomarker for ICC under NMS stress. nNOS expression was only observed in the ICC of the submucosal plexus of IBS-D mice, and the inhibition of nNOS changed the phenotype from IBS-D to IBS with constipation.

Conclusion: Our study demonstrates that early-life stress can influence to ICC and modulate bowel activity and that nNOS might be used as a biomarker for ICC stimulation in IBS.
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http://dx.doi.org/10.1007/s10620-018-4933-7DOI Listing
March 2018

CD80CD86 deficiency disrupts regulatory CD4+FoxP3+T cell homoeostasis and induces autoimmune-like alopecia.

Exp Dermatol 2017 11 3;26(11):1053-1059. Epub 2017 Jul 3.

Designed Animal and Transplantation Research Institute, Greenbio Research and Technology, Seoul National University, Pyeongchang, Kangwon, Korea.

Alopecia areata (AA) is an autoimmune disease that results in spot baldness in humans. Adequate animal models for AA are currently lacking. The objective of this study was to elucidate the mechanism of autoimmune-like alopecia (ALA) in C57BL/6.CD80CD86-deficient (B6.CD80CD86 ) mice. Incidence and severity of alopecia were analysed in 58 B6.CD80CD86 mice using histological examination, flow cytometry, multiplex enzyme-linked immunosorbent assay, quantitative RT-PCR and CD25 inhibition test. Both male and female B6.CD80CD86 mice showed almost 100% incidence of hair loss at 40 weeks of age. Moreover, CD4+FoxP3+Treg (Treg) cell population in B6.CD80CD86 mice was significantly lower than in B6 mice, which presumably underlined autoimmune reaction. Histologically, B6.CD80CD86 mice showed CD4+ and CD8+ T-cell infiltration around terminal follicle region and exhibited hair follicle destruction in the anagen or catagen stage. Negative correlation between the number of CD4+FoxP3+ Tregs and ALA was confirmed by the CD25 depletion test in B6 mice, as follicle destruction was similar to that observed in B6.CD80CD86 animals. CD80CD86 deficiency disrupted CD4+FoxP3+ Treg homoeostasis and prompted the development of ALA. We demonstrated that B6.CD80CD86 mice might have several advantages as an ALA model, because they exhibited high incidence of disease phenotype and epipathogenesis similar to that observed in human AA.
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http://dx.doi.org/10.1111/exd.13371DOI Listing
November 2017

5-Bromo-2'-deoxyuridine induced effluvium via p53-mediated CD326-positive keratinocyte apoptosis in C57BL/6 mice.

J Dermatol 2017 Feb 20;44(2):180-185. Epub 2016 Aug 20.

Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang, Korea.

Anagen effluvium develops because of disturbances in the hair follicle cycle, leading to acute and severe hair loss in humans. The objective of this study was to establish a mouse model of anagen effluvium by 5-bromo-2'-deoxyuridine (BrdU) treatment, and evaluate the pathological changes and underlying mechanisms. We treated 9-10-day-old pups and 3-7-week-old C57BL/6 mice with BrdU. After successfully inducing hair loss in the neonatal pups, microscopic, immunohistochemical and flow cytometry analyses were conducted. BrdU induced early onset alopecia in neonates and caused epidermal thickening and hair shaft breakage. BrdU appeared to incorporate the CD326-positive keratinocyte layer and induced p53-related apoptosis. Keratinocyte apoptosis caused immune cell infiltration in the dermal region; M2 macrophages and neutrophils were dominant. The BrdU-induced hair loss was dose-dependent, and alopecia was visible at a dose range of 25-200 μg/g bodyweight. The BrdU-induced anagen effluvium mouse model is novel and easily established by administrating four simple BrdU injections to pups; these mice showed synchronized onset of alopecia symptoms with little individual variation. Moreover, this model showed an alopecia phenotype similar to that of human anagen effluvium with acute, severe and widespread hair loss.
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http://dx.doi.org/10.1111/1346-8138.13547DOI Listing
February 2017

Premature mammary gland involution with repeated corticosterone injection in interleukin 10-deficient mice.

Biosci Biotechnol Biochem 2016 Dec 3;80(12):2318-2324. Epub 2016 Aug 3.

b Graduate School of International Agricultural Technology , Seoul National University , Pyeongchang , Korea.

Recently, we found that maternal stress could induce premature mammary gland involution in interleukin 10 knock out (IL-10) mice. To elucidate correlation between stress, IL-10, and mammary gland involution, corticosterone was injected into the lactating wild type and IL-10-deficient mice and assessed mammary gland phenotype. Repetitive corticosterone injection developed premature mammary gland involution only in B6.IL-10 mice; moreover, it induced alopecia in nursing pups. Corticosterone injection induced several typical changes such as mammary gland epithelial cell apoptosis, macrophage infiltration, fat deposition in adipocyte, STAT3 phosphorylation, and upregulation of tyrosine hydroxylase gene in adrenal gland. Overall incidence of pup alopecia and mammary gland involution was relatively high in corticosterone than control B6.IL-10 group (57% vs. 20%). Our finding demonstrates that IL-10 is important for stress modulation, and B6.Il-10 with corticosterone has several advantage such as simple to establish, well-defined onset of mammary gland involution, high incidence, and inducing pup alopecia.
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http://dx.doi.org/10.1080/09168451.2016.1214556DOI Listing
December 2016

Instant noodles, processed food intake, and dietary pattern are associated with atopic dermatitis in an adult population (KNHANES 2009-2011).

Asia Pac J Clin Nutr 2016 ;25(3):602-13

Deptartment of Food Science and Nutrition, Keimyung University, Daegu, Korea. Email:

Background And Objectives: The incidence of atopic dermatitis (AD) is continuously increasing in industrialized countries, possibly due to dietary and lifestyle changes. However, the association between processed food intake and AD has not been studied in a large adult population.

Methods And Study Design: We investigated the association between dietary habits and AD in 17,497 adults in the 2009-2011 Korean National Health and Nutrition Examination Survey (KNHANES).

Results: We identified 4 dietary patterns using principal components analysis of a 63-item food frequency questionnaire: the "traditional dietary pattern", rich in rice and kimchi; the "processed food pattern", with more meat, instant noodles, soda, and processed foods; the "healthy dietary pattern", high in grains, vegetables, fruits, and seaweeds; and the "drinking dietary pattern", mainly drinking coffee and alcohol. Adjusted odds ratios (ORs) for AD were calculated according to dietary patterns after adjusting for potential confounders with incorporation of sample weights for the complex sample design. The "meat and processed food" pattern was associated with a significant 1.57 fold higher OR for atopic dermatitis than the low consumption group. Further analysis revealed that the increased atopic dermatitis was most closely associated with instant noodles. In contrast, the groups with high intake of rice and kimchi exhibited lower ORs, 0.38 and 0.43 folds, compared to the low intake group.

Conclusion: Consuming instant noodles, meat and processed foods was associated with increased prevalence of atopic dermatitis, whereas consuming rice and kimchi, and coffee was associated with decreased prevalence of atopic dermatitis.
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http://dx.doi.org/10.6133/apjcn.092015.23DOI Listing
September 2016

Vitamin D and atopic dermatitis: A systematic review and meta-analysis.

Nutrition 2016 Sep 18;32(9):913-20. Epub 2016 Feb 18.

Department of Food Science and Nutrition, Keimyung University, Daegu, South Korea. Electronic address:

Objectives: Despite the evidence supporting the use of vitamin D supplements for managing atopic dermatitis (AD), no meta-analysis providing definite conclusions in this field has been reported. The purpose of the present study was to conduct a systematic review and meta-analysis of all controlled studies of vitamin D for treating AD to elucidate the efficacy of vitamin D for alleviating the symptoms of AD.

Methods: Literature searches were conducted using Ovid-MEDLINE, EMBASE, Web of Science, Cochrane Library, and Korean and Chinese databases. Search terms used were "vitamin D", "atopic dermatitis", "randomized", "controlled trial", and "clinical trial". Random effects models were used to calculate the mean difference, with 95% confidence intervals to analyze the effects of vitamin D supplementation for severity of AD.

Results: Initial searches yielded 266 citations. Of these original results, nine met specific selection criteria. Four of the randomized controlled trials compared the efficacy of vitamin D with a placebo on severity of AD and were included in the meta-analysis. The vitamin D supplementation interventions showed a higher mean difference in severity of AD symptoms (mean difference = -5.81, 95% CI: -9.03 to -2.59, P = 0.0004, I(2) = 50%).

Conclusions: Vitamin D has a potentially significant role for improving the symptoms of AD. The results from this study suggest that vitamin D supplementation may help ameliorate the severity of AD, and can be considered as a safe and tolerable therapy. However, larger-scale studies over a longer duration of treatment are needed to confirm this conclusion.
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http://dx.doi.org/10.1016/j.nut.2016.01.023DOI Listing
September 2016

Fermented food intake is associated with a reduced likelihood of atopic dermatitis in an adult population (Korean National Health and Nutrition Examination Survey 2012-2013).

Nutr Res 2016 Feb 19;36(2):125-33. Epub 2015 Nov 19.

Department of Food Science and Nutrition, Keimyung University, Daegu, Republic of Korea. Electronic address:

The prevalence of atopic dermatitis (AD) has continuously increased throughout the world in every age group, and the recent increase in AD in Korean adults may be related to changes in nutrient intakes due to westernization of dietary patterns. We hypothesized that the prevalence of AD is associated with the different dietary patterns and fermented food intakes of the Korean adult population. We examined the hypothesis using 9763 adults 19 years or older using the 2012-2013 Korean National Health and Nutrition Examination Survey. We identified 4 dietary patterns in addition to that including fermented foods using principal component analysis on data obtained from a 116-item validated semiquantitative food frequency questionnaire: meat and processed foods; vegetables, fruits, legumes, seafood, and seaweed; rice and grains; and coffee, chocolate, and ice cream. Adjusted odds ratios (ORs) for AD were calculated according to dietary patterns after adjusting for potential confounders. High levels of consumption (>92 times/month) of fermented foods such as doenjang, chungkookjang, kimchi, fermented seafood, makgeolli, and beer were associated with a lower prevalence of AD (OR, 0.56; 95% confidence interval [CI], 0.37-0.84). In contrast, high levels of consumption of meat and processed foods were strongly associated with the prevalence of AD (OR, 2.42; 95% CI, 1.48-3.94). Interestingly, the consumption of coffee, chocolate, and ice cream was significantly negatively associated with the prevalence of AD (OR, 0.53; 95% CI, 0.34-0.82). In conclusion, the hypothesis was accepted. The results can be applied to nutrition education programs for the general population to decrease risk factors for AD.
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http://dx.doi.org/10.1016/j.nutres.2015.11.011DOI Listing
February 2016

Accuracy of residual apnea-hypopnea index obtained using the continuous positive airway pressure device: application of new version 2.0 scoring rules for respiratory events during sleep.

Sleep Breath 2015 Dec 25;19(4):1335-41. Epub 2015 Sep 25.

Sleep Disorders Center, Department of Neurology, Cheonan Hospital, Soonchunhyang University College of Medicine, Soonchunhyang 6 gil 31, Dongnam-gu, Cheonan, Chungnam, 330-721, South Korea.

Purpose: Continuous positive airway pressure (CPAP) devices can estimate apnea-hypopnea index (AHI) using respiratory event detection algorithms. In 2012, rules for manually scoring respiratory events during sleep were updated to version 2.0. The purpose of the present study was to compare residual AHI determined using the Sleepstyle HC608 CPAP device (HC) with those determined by the new manual scoring (NM) rules during CPAP titration in patients with obstructive sleep apnea (OSA).

Methods: Fifty-seven patients underwent CPAP titration with HC. Correlations were assessed between AHI determined by NM and HC. The AHI, the apnea index (AI), and the hypopnea index (HI) were evaluated separately.

Results: The mean AHI as assessed using diagnostic polysomnography (PSG) was 53.9 ± 22.4. During CPAP titration, respiratory events were effectively suppressed (HC-AHI, 4.2 ± 6.0; NM-AHI, 6.0 ± 5.8). Lower HI and AHI were obtained using HC compared to NM (HC-HI, 2.9 ± 3.6 and NM-HI, 5.2 ± 4.2, p < 0.001; HC-AHI, 4.2 ± 6.0 and NM-AHI, 6.0 ± 5.8, p < 0.001). Additionally, HC reported higher AI compared to NM (HC-AI, 1.3 ± 2.8; NM-AI, 0.9 ± 2.2, p = 0.002). NM-AI (ß = 1.017, p < 0.001), NM-HI (ß = -0.599, p < 0.001), and NM-arousal index (ß = -0.058, p = 0.042) were associated with greater differences between HC-AHI and NM-AHI in multivariate regression analysis.

Conclusions: Our findings indicate differences in scoring respiratory events between our CPAP device and new version 2.0 manual scoring and suggest that residual AHI values should be carefully interpreted.
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http://dx.doi.org/10.1007/s11325-015-1257-0DOI Listing
December 2015

Probiotics for weight loss: a systematic review and meta-analysis.

Nutr Res 2015 Jul 21;35(7):566-75. Epub 2015 May 21.

Department of Food Science and Nutrition, Keimyung University, Daegu, Korea. Electronic address:

The intestinal microbiota has been reported to be one of the potential determinants of obesity in recent human and animal studies. Probiotics may affect the gut microbiota to modulate obesity. This systematic review aims to summarize and critically evaluate the evidence from clinical trials that have tested the effectiveness of probiotics or foods containing probiotics as a treatment for weight loss. Literature searches of electronic databases such as PubMed, Cochrane Library, and EMBASE were conducted. Methodological quality was assessed using body weight and body mass index (BMI). Initial searches yielded 368 articles. Of these, only 9 met the selection criteria. Because of insufficient data, only 4 of the studies were randomized controlled trials (RCTs) that compared the therapeutic efficacy of probiotics with placebo. The meta-analysis of these data showed no significant effect of probiotics on body weight and BMI (body weight, n = 196; mean difference, -1.77; 95% confidence interval, -4.84 to 1.29; P = .26; BMI, n = 154; mean difference, 0.77; 95% confidence interval, -0.24 to 1.78; P = .14). However, the total number of RCTs included in the analysis, the total sample size, and the methodological quality of the primary studies were too low to draw definitive conclusions. Thus, more rigorously designed RCTs are necessary to examine the effect of probiotics on body weight in greater detail. Collectively, the RCTs examined in this meta-analysis indicated that probiotics have limited efficacy in terms of decreasing body weight and BMI and were not effective for weight loss.
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http://dx.doi.org/10.1016/j.nutres.2015.05.008DOI Listing
July 2015

Alveolar ridge preservation of an extraction socket using autogenous tooth bone graft material for implant site development: prospective case series.

J Adv Prosthodont 2014 Dec 17;6(6):521-7. Epub 2014 Dec 17.

Department of Periodontology, School of Dentistry, Seoul National University, Seoul, Republic of Korea.

This case series evaluated the clinical efficacy of autogenous tooth bone graft material (AutoBT) in alveolar ridge preservation of an extraction socket. Thirteen patients who received extraction socket graft using AutoBT followed by delayed implant placements from Nov. 2008 to Aug. 2010 were evaluated. A total of fifteen implants were placed. The primary and secondary stability of the placed implants were an average of 58 ISQ and 77.9 ISQ, respectively. The average amount of crestal bone loss around the implant was 0.05 mm during an average of 22.5 months (from 12 to 34 months) of functional loading. Newly formed tissues were evident from the 3-month specimen. Within the limitations of this case, autogenous tooth bone graft material can be a favorable bone substitute for extraction socket graft due to its good bone remodeling and osteoconductivity.
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http://dx.doi.org/10.4047/jap.2014.6.6.521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279052PMC
December 2014

Desensitizing toothpaste versus placebo for dentin hypersensitivity: a systematic review and meta-analysis.

J Clin Periodontol 2015 Feb 9;42(2):131-41. Epub 2015 Jan 9.

Department of Conservative Dentistry, Section of Dentistry, Seoul National University Bundang Hospital, Seongnam, Korea.

Aim: The aim is to assess the effect of desensitizing toothpaste on dentin hypersensitivity.

Methods: We searched PubMed, CENTRAL, and Embase on December 20, 2013.

Results: Out of the 626 articles searched, a total of 31 randomized controlled clinical trials were included. The Standardized mean differences (SMD) for potassium-containing toothpaste (n = 8) was -1.28 (95% Confidence interval (CI) -2.05 to -0.51; I(2) = 93%); Stannous fluoride- (n = 6) was -1.37 (95% CI, -2.30 to -0.44; I(2) = 95%); Potassium and stannous fluoride- (n = 3) was -2.50 (95% CI, -4.10 to -0.91; I(2) = 95%); Calcium sodium phosphosilicate- (n = 4) was -2.36 (95% CI, -3.72 to -1.00; I(2) = 92%); Arginine- (n = 8) was -3.25 (95% CI, -3.87 to -2.63; I(2) = 86%). The desensitizing effect was favoured in the intervention group treated with potassium-, stannous fluoride-, potassium and stannous fluoride-, calcium sodium phosphosilicate-, and arginine-containing toothpaste compared to placebo. Whereas, strontium-containing toothpaste (SMD, 0.05; 95% CI, -0.34 to 0.44; I(2) = 64%) was found to have no statistically significant desensitizing effect in the meta-analysis of four studies.

Conclusions: The study reports that there is sufficient evidence to support the use of potassium-, stannous fluoride-, potassium and stannous fluoride-, calcium sodium phosphosilicate-, and arginine-containing desensitizing toothpastes for dentin hypersensitivity, but not the use of strontium-containing desensitizing toothpaste.
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http://dx.doi.org/10.1111/jcpe.12347DOI Listing
February 2015

Chemical regulation of signaling pathways to programmed necrosis.

Arch Pharm Res 2014 Jun 10;37(6):689-97. Epub 2014 Apr 10.

College of Pharmacy, Keimyung University, 1000 Sindang-dong, Dalseo-gu, Daegu, 704-701, South Korea.

Necroptosis is an active and well-orchestrated necrosis, distinctive from apoptosis in microscopic structure, and biochemical and molecular features. Unlike apoptosis-undergoing cells, which are removed by macrophage or neighboring cells, necrotic cell death releases danger signals and provokes inflammation, and further a severe damage to neighbor tissue. A regulated necrosis, termed as necroptosis or programmed necrosis, is emerging as a new paradigm of cell death that can be activated when apoptotic machinery is genetically or pathogenically defective. It plays biological significances in pathogenesis of a variety of inflammatory diseases as well as in a beneficial innate immune defense mechanism. This review highlights the identification of hits against necroptosis, and comprehensive approaches to discovery of small molecules that regulate necroptotic cell death. Also, the signaling molecular mechanism of necroptosis and future clinical uses of necroptosis inhibitor will be described in brief.
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http://dx.doi.org/10.1007/s12272-014-0385-6DOI Listing
June 2014

Guided bone regeneration using autogenous tooth bone graft in implant therapy: case series.

Implant Dent 2014 Apr;23(2):138-43

*Professor, Department of Oral and Maxillofacial Surgery, Seoul National University Bundang Hospital, Seongnam, Republic of Korea. †Professor, Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Republic of Korea. ‡Assistant Professor, Department of Conservative Dentistry, Seoul National University Bundang Hospital, Seongnam, Republic of Korea. §CTO, R&D Director, Korea Tooth Bank, Republic of Korea. ‖Assistant Professor, Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Republic of Korea. ¶Fellow, Department of Oral and Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Republic of Korea.

Recently, techniques have been reported that involve the preparation of extracted teeth from patients used as particulated bone graft materials for bone graft purposes. For implant placement and bone graft, autogenous teeth bone graft materials were used in 15 patients, and clinically excellent results were obtained. In histological examination, favorable bony healing by osteoconduction was observed.
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http://dx.doi.org/10.1097/ID.0000000000000046DOI Listing
April 2014

Effect of loading time on marginal bone loss around hydroxyapatite-coated implants.

J Korean Assoc Oral Maxillofac Surg 2013 Aug 23;39(4):161-7. Epub 2013 Aug 23.

Department of Conservative Dentistry, Section of Dentistry, Seoul National University Bundang Hospital, Seongnam, Korea.

Objectives: The objective of this study is compare the rate of marginal bone resorption around hydroxyapatite-coated implants given different loading times in order to evaluate their stability.

Materials And Methods: The study was conducted retrospectively for one year, targeting 41 patients whose treatment areas were the posterior maxilla and the mandible. Osstem TS III HA (Osstem Implant Co., Busan, Korea) and Zimmer TSV-HA (Zimmer Dental, Carlsbad, CA, USA), which employ the new hydroxyapatite coating technique, were used. The patients were divided into two groups - immediate and delayed loading - and the bone level at the time of loading commencement and after one year of loading was measured using periapical radiography. Differences between the groups were evaluated using Mann-Whitney (α=0.05).

Results: For all patients as a single group, the survival rate of the implants was 100%, and the mean marginal bone loss was 0.26±0.59 mm. In comparison of the differences by loading, mean marginal bone loss of 0.32±0.69 mm was recorded for the immediate loading group whereas the delayed loading group had mean marginal bone loss of 0.16±0.42 mm. However, the difference was not significant (P>0.05).

Conclusion: Within the limited observation period of one year, predictable survival rates can be expected when using immediately loaded hydroxyapatite-coated implants.
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http://dx.doi.org/10.5125/jkaoms.2013.39.4.161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858130PMC
August 2013

Retrospective clinical study on sinus bone graft and tapered-body implant placement.

J Korean Assoc Oral Maxillofac Surg 2013 Apr 23;39(2):77-84. Epub 2013 Apr 23.

Department of Conservative Dentistry, Seoul National University Bundang Hospital, Seongnam, Korea.

Objectives: This study evaluated implant success rate, survival rate, marginal bone resorption of implants, and material resorption of sinus bone graft in cases wherein tapered body implants were installed.

Materials And Methods: From September 2003 to January 2006, 20 patients from Seoul National University Bundong Hospital, with a mean age of 54.7 years, were considered. The mean follow-up period was 19 months. This study covered 50 implants; 14 implants were placed in the maxillary premolar area, and 36 in the maxillary molar area; 24 sinuses were included.

Results: The success rate was 92%, and the survival rate was 96.0%. The mean amount of sinus augmentation was 12.35±3.27 mm. The bone graft resorption rate one year after surgery was 0.97±0.84 mm; that for the immediate implantation group was 0.91±0.86 mm, and that for the delayed implantation group was 1.16±0.77 mm. However, the difference was not statistically significant. The mean marginal bone resorption one year after restoration was 0.17±0.27 mm (immediate group: 0.12±0.23 mm; delayed group 0.40±0.33 mm); statistically significant difference was observed between the two groups.

Conclusion: Tapered body implant can be available in the maxillary posterior edentulous ridge which sinus bone graft is necessary.
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http://dx.doi.org/10.5125/jkaoms.2013.39.2.77DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3858150PMC
April 2013

One-Year Prospective Study of 7-mm-Long Implants in the Mandible: Installation Technique and Crown/Implant Ratio of 1.5 or Less.

J Oral Implantol 2015 Apr 3;41(2):e30-5. Epub 2013 Dec 3.

1  Department of Oral and Maxillofacial Surgery, Seoul National University Bundang Hospital, Gyeonggi-do, Korea.

This study prospectively evaluated the clinical prognoses of short implants (7-mm long) in the mandible. We investigated the clinical prognosis of short implants in 20 patients (46 implants) according to the installation technique (submerged or nonsubmerged), installation depth (5.5-mm depth, 7-mm depth), and crown/implant ratio. We investigated the marginal bone loss and peri-implant soft-tissue index 12 months after the final prosthetic delivery. Twelve months after prosthetic delivery, no statistically significant differences were observed in bone loss in relation to the type of installation technique, installation depth, or crown/implant ratio. The plaque index and pocket depth indexes were not influenced by the installation technique, installation depth, or crown/implant ratio. We observed marginal bone loss of 3.3 mm in 1 implant from the nonsubmerged group. The total 1-year success rate was 97.83%. Based on this 1-year success rate, short implants had a good clinical prognosis, regardless of the installation technique, installation depth, or crown/implant ratio.
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http://dx.doi.org/10.1563/AAID-JOI-D-13-00162DOI Listing
April 2015

Cortisone induces insulin resistance in C2C12 myotubes through activation of 11beta-hydroxysteroid dehydrogenase 1 and autocrinal regulation.

Cell Biochem Funct 2014 Apr 7;32(3):249-57. Epub 2013 Oct 7.

College of Pharmacy, Keimyung University, Daegu, South Korea.

The enzyme 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) is known to catalyse inactive glucocorticoids into active forms, and its dysregulation in adipose and muscle tissues has been implicated in the development of metabolic syndrome. To delineate the molecular mechanism by which active cortisol has an antagonizing effect against insulin, we optimized the metabolic production of cortisol and its biological functions in myotubes (C2C12). Myotubes supplemented with cortisone actively catalysed its conversion into cortisol, which in turn abolished phosphorylation of Akt in response to insulin treatment. This led to diminished uptake of insulin-induced glucose. This was corroborated by the application of 11β-HSD1 inhibitor glycyrrhetinic acid and a glucocorticoid receptor antagonist RU-486, which reversed completely the antagonizing effects of cortisol on insulin action. Therefore, development of specific inhibitors targeting 11β-HSD1 might be a promising way to improve impaired insulin-stimulated glucose uptake.
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http://dx.doi.org/10.1002/cbf.3008DOI Listing
April 2014

Estrogen-like activity of aqueous extract from Agrimonia pilosa Ledeb. in MCF-7 cells.

BMC Complement Altern Med 2012 Dec 21;12:260. Epub 2012 Dec 21.

Functional Food & Nutrition Division, Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration, Suwon, Republic of Korea.

Background: Postmenopausal women experience estrogen deficiency-related menopausal symptoms (e.g., hot flashes and mood swings) and a dramatic increase in the incidence of chronic diseases. Although estrogen-replacement therapy (ERT) can reduce mortality from cardiovascular disease and improve osteoporosis and menopausal symptoms, its side effects have limited recent use. This study investigated the estrogen-like activity of aqueous extract from Agrimonia pilosa Ledeb.

Methods: The estrogenic activity of A. pilosa was investigated by using several in vitro assays. The binding activity of A. pilosa on estrogen receptors was examined using a fluorescence polarization-based competitive binding assay. The proliferative activity of A. pilosa was also examined using MCF-7 cells. Furthermore, the effect of A. pilosa on the expression of 3 estrogen-dependent genes was assessed.

Results: Using liquid chromatography-mass spectrometry, the 3 major peaks of A. pilosa aqueous extract were identified as apigenin-hexose, luteolin-glucuronide, and apigenin-glucuronide. The aqueous extract induced the proliferation of estrogen receptor-positive MCF-7 cells (p < 0.05). A. pilosa-stimulated proliferation was blocked on adding the estrogen antagonist ICI 182,780. Moreover, A. pilosa treatment increased the mRNA expression of the estrogen-responsive genes pS2 and PR (p < 0.05).

Conclusions: These results suggest A. pilosa can be used to improve estrogen deficiency-related menopausal symptoms or to treat diseases in postmenopausal women.
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http://dx.doi.org/10.1186/1472-6882-12-260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3575283PMC
December 2012

Analysis of the cytokine profiles of the synovial fluid in a normal temporomandibular joint: preliminary study.

J Craniomaxillofac Surg 2012 Dec 16;40(8):e337-41. Epub 2012 Mar 16.

Department of Oral and Maxillofacial Surgery, Section of Dentistry, Seoul National University Bundang Hospital, Republic of Korea.

The purpose of this study was to compare the cytokine profiles of the synovial fluid from the temporomandibular joint (TMJ) spaces of normal individuals and temporomandibular disorder (TMD) patients. Thirty-four patients with planned orthognathic surgery did not present abnormalities of the TMJ on magnetic resonance images and radiographs and did not show the symptoms identified by the Research Diagnostic Criteria for TMD (RDC-TMD); as a result, they were assigned to the control group. Twenty-two patients who sought treatment for TMD during the same period were assigned to the TMD group. Synovial fluid was collected from superior TMJ spaces, and cytokine expression was analysed by an enzyme-linked immunosorbent assay (ELISA). Significant differences were tested using Fisher's exact test (p<0.05). Granulocyte Macrophage Colony stimulating Factor (GM-CSF), interferon (INF), interleukin (IL)-1β, IL-2, IL-6, IL-8, IL-10 and tumour necrosis factor (TNF)-α were detected in the TMD group, whereas no cytokines were detected in the control group. The most prevalent cytokines in the TMD group were IL-1β, IL-6 and GM-CSF. IL-4 and IL-5 were not detected in either the TMD group or in the control group. None of the cytokines that were detected in patients with TMD were found in the articular spaces of normal individuals.
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http://dx.doi.org/10.1016/j.jcms.2012.02.002DOI Listing
December 2012