Publications by authors named "Ji Young Kim"

1,134 Publications

  • Page 1 of 1

Nephrin expression in human epidermal keratinocytes and its implication in poor wound closure.

FASEB J 2022 Jul;36(7):e22424

Department of Dermatology and Cutaneous Biology Research Institute, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea.

Nephrin is a type-1 transmembrane protein and a component of the slit diaphragm renal-filtration barrier. It has several functions in actin remodeling and cell-cell adhesion. Nephrin is principally located in the kidney glomerulus, but several studies have reported that nephrin is found in the pancreas, brain, and placenta. However, nephrin expression and its role in human skin have not yet been reported. First, using single-cell RNA sequencing, immunohistochemistry, and immuno-electron microscopy, nephrin expression was confirmed in human-skin epidermal keratinocytes. Nephrin expression colocalized with the expression of zonula occludens-1 in keratinocytes and was closely related to keratinocyte cell density, proliferation, and migration. High glucose treatment decreased nephrin expression and compromised keratinocyte cell migration without yes-associated protein nuclear entry. This reduced cell migration under high glucose conditions was improved in nephrin-overexpressing keratinocytes. Nephrin was highly expressed on the margins of re-epithelized epidermis based on in vivo mice and ex vivo human skin wound models. The results demonstrate that nephrin is expressed in human-skin keratinocytes and functions in cell adhesion, proliferation, and migration. In conclusion, this study suggests that nephrin may have a variety of physiological roles in human skin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.202100455RRDOI Listing
July 2022

[email protected] Core-Shell Structured Hybrid Anode Materials for Lithium-Ion Batteries.

Nanomaterials (Basel) 2022 Jun 10;12(12). Epub 2022 Jun 10.

Department of Energy & Materials Engineering, Dongguk University, Seoul 04620, Korea.

We explore a phase engineering strategy to improve the electrochemical performance of transition metal sulfides (TMSs) in anode materials for lithium-ion batteries (LIBs). A one-pot hydrothermal approach has been employed to synthesize MoS nanostructures. MoS and MoO phases can be readily controlled by straightforward calcination in the (200-300) °C temperature range. An optimized temperature of 250 °C yields a phase-engineered [email protected] hybrid, while 200 and 300 °C produce single MoS and MoO phases. When tested in LIBs anode, the optimized [email protected] hybrid outperforms the pristine MoS and MoO counterparts. With above 99% Coulombic efficiency (CE), the hybrid anode retains its capacity of 564 mAh g after 100 cycles, and maintains a capacity of 278 mAh g at 700 mA g current density. These favorable characteristics are attributed to the formation of MoO passivation surface layer on MoS and reactive interfaces between the two phases, which facilitate the Li-ion insertion/extraction, successively improving [email protected] anode performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nano12122008DOI Listing
June 2022

Pushing the Boundaries of Multicomponent Alloy Nanostructures: Hybrid Approach of Liquid Phase Separation and Selective Leaching Processes.

Acc Chem Res 2022 Jun 17. Epub 2022 Jun 17.

Department of Materials Science and Engineering, Research Institute of Advanced Materials & Institute of Engineering Research, Seoul National University, Seoul 08826, Republic of Korea.

ConspectusAlloying, or mixing of multiple metallic elements, is the classical way of novel materials development since the Bronze age. Increased numbers of principal elements expand the compositional space for alloy design vastly, leading to nearly endless possibilities of unexpected and unique materials properties. In contrast to bulk alloying processes represented by casting of molten metal mixtures, the fabrication of multicomponent alloy (MCA) nanostructures such as nanoparticles and nanofoams with more than three elements is often challenging, and a few methodologies for directly synthesizing alloy nanostructures up to denary systems have been suggested recently. However, forming alloy nanoparticles inside another metal matrix, instead of inside aqueous media in wet-chemical synthesis, is a fairly well understood strategy in terms of physical metallurgy. Extracting those alloy nanophases from the matrix could provide an alternative way to fabricate novel MCA nanostructures.In this Account, we describe a hybrid approach of metallurgical bottom-up and chemical top-down processes for fabricating MCA nanostructures including nanoparticles and nanofoams. The former utilizes a liquid-state phase separation process that resembles "oil and water" but occurs at the nanoscale due to thermodynamic mixing relations among alloying elements and a rapid quenching process. Thermodynamic prediction of the immiscible boundary in a temperature-composition space (miscibility gap) plays a key role in designing precursor alloys for MCA nanostructures. Selective leaching, the chemical top-down process for extracting the alloy nanostructures from the precursors, uses the chemical reactivity difference between the embedded nanostructures and the matrix phase against a certain chemical solution. We discuss here that the precise control of alloy composition and cooling rate based on thermodynamic assessments enables researchers to prepare phase-separating precursor alloys for fabricating both nanoparticles and nanofoams with a broad size range from a few nanometers to a few hundred nanometers. Depending on the alloy systems, the atomic structure of alloy nanostructures could be controlled from fully amorphous to nanocrystalline and even to quasicrystalline structure. We demonstrate how the different sizes of alloy nanostructures fabricated by a single hybrid procedure can be effectively exploited for investigating size-dependent physical properties. The future and potential research directions for this hybrid approach are also briefly discussed. This unique approach for fabricating nanosized alloys provides an extended methodology to discover novel metallic nanomaterials with promising properties in diverse compositional spaces of MCA systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.accounts.2c00143DOI Listing
June 2022

Topology Optimized Prelithiated SiO Anode Materials for Lithium-Ion Batteries.

Small 2022 Jun 9:e2202209. Epub 2022 Jun 9.

Department of Energy Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul, 04763, Republic of Korea.

Silicon monoxide (SiO)-based materials have great potential as high-capacity anode materials for lithium-ion batteries. However, they suffer from a low initial coulombic efficiency (ICE) and poor cycle stability, which prevent their successful implementation into commercial lithium-ion batteries. Despite considerable efforts in recent decades, their low ICE and poor cycle stability cannot be resolved at the same time. Here, it is demonstrated that the topological optimization of the prelithiated SiO materials is highly effective in improving both ICE and capacity retention. Laser-assisted atom probe tomography combined with thermogravimetry and differential scanning calorimetry reveals that two exothermic reactions related to microstructural evolution are key in optimizing the domain size of the Si active phase and Li SiO buffer phase, and their topological arrangements in prelithiated SiO materials. The optimized prelithiated SiO, heat-treated at 650 °C, shows higher capacity retention of 73.4% and lower thickness changes of 68% after 300 cycles than those treated at other temperatures, with high ICE of ≈90% and reversible capacity of 1164 mAh g . Such excellent electrochemical properties of the prelithiated SiO electrode originate from its optimized topological arrangement of active Si phase and Li SiO inactive buffer phase.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202202209DOI Listing
June 2022

Stratifin (SFN) regulates lung cancer progression via nucleating the Vps34-BECN1-TRAF6 complex for autophagy induction.

Clin Transl Med 2022 Jun;12(6):e896

Department of Immunology and Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/ctm2.896DOI Listing
June 2022

Potential Therapeutic Role of Bone Morphogenic Protein 7 (BMP7) in the Pathogenesis of Graves' Orbitopathy.

Invest Ophthalmol Vis Sci 2022 06;63(6)

Department of Ophthalmology, Severance Hospital, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea.

Purpose: We investigated a role of bone morphogenic protein 7 (BMP7), a member of the TGF-β superfamily on pathogenic mechanism of Graves' orbitopathy (GO). The therapeutic effects of BMP7 on inflammation and fibrosis were evaluated in cultured Graves' orbital fibroblasts.

Methods: Expression of BMP7 was compared in cultured orbital tissue explants from GO (n = 12) and normal control (n = 12) subjects using real-time PCR. Orbital fibroblasts were cultured from orbital connective tissues obtained from GO (n = 3) and normal control patients (n = 3). Cells were pretreated with recombinant human BMP7 (rhBMP7) before stimulation with TGF-β, IL-1β, and TNF-α. Fibrosis-related proteins and inflammatory cytokines were analyzed by Western blotting. The activation of signaling molecules in inflammation and fibrosis was also analyzed.

Results: The expressions of BMP7 mRNA were lower in GO orbital tissues than control. Fibrosis-related proteins, fibronectin, collagen 1α, and α-SMA induced by TGF-β were suppressed by treating rhBMP7, and rhBMP7 upregulated TGF-β induced SMAD1/5/8 protein expression, whereas downregulated SMAD2/3. Increased pro-inflammatory molecules, IL-6, IL-8, and intercellular adhesion molecule-1 (ICAM-1) by IL-1β or TNF-α were blocked by rhBMP7 treatment, and the expression of phosphorylated NFκB and Akt was suppressed by rhBMP7 treatment.

Conclusions: BMP7 transcript levels were downregulated in Graves' orbital tissues. Exogenous BMP7 treatment showed inhibitory effects on the production of profibrotic proteins and proinflammatory cytokines in orbital fibroblasts. Our results provide a molecular basis of BMP7 as a new potential therapeutic agent through the opposing mechanism of profibrotic TGF-β/SMAD signaling and proinflammatory cytokine production.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1167/iovs.63.6.7DOI Listing
June 2022

Clinical outcomes of gastrointestinal bleeding management during anticoagulation therapy.

PLoS One 2022 3;17(6):e0269262. Epub 2022 Jun 3.

Department of Neurology, Inje University Seoul Paik Hospital, Seoul, Republic of Korea.

Background: Acute gastrointestinal (GI) bleeding is not an uncommon complication of oral anticoagulation (OAC) therapy that requires medication cessation. However, drug cessation may cause fatal stroke or systemic embolization in patients at high thromboembolic risk. Here we sought to find an appropriate anticoagulation cessation strategy in cases of GI bleeding during OAC therapy.

Methods: This single-center retrospective cohort analysis was performed between 2010 and 2018. Patients were enrolled if the following three consecutive conditions were met: 1) electrocardiography electrocardiography-proven atrial fibrillation; 2) OAC therapy; and 3) GI bleeding. We divided the drug cessation strategy into the continuation and discontinuation groups. During 1-year follow-up, the rates of major thromboembolic and rebleeding events were calculated.

Results: One hundred and forty-six patients (continuation [n = 54] vs. discontinuation [n = 92] group) were enrolled. Patients in the discontinuation group were more likely to be older (69.8 ± 9.0 yrs vs. 74.9 ± 8.9 yrs, p = 0.001), while patients in the continuation group were more likely to have undergone cardiac valve surgery (51.9% vs. 20.7%, p<0.001). The presence of a mechanical mitral valve was a determinant of continuation strategy (38.9% vs. 7.5%, p<0.001). However, the mean CHA₂DS₂-VASc (3.4±1.3 vs. 4.1±1.6, p = 0.010) and Glasgow-Blatchford (8.0±2.4 vs. 8.9±2.5, p = 0.037) scores were higher in the discontinuation group. Two major embolic strokes occurred in each group (3.7% vs. 2.2%, p = 0.585). Four of 54 (7.4%) and five of 92 (5.4%) patients had rebleeding events during follow-up (p = 0.632). One embolic event in the continuation group and one rebleeding event in the discontinuation group were fatal. The Glasgow-Blatchford score was a predictor of 1-year rebleeding events (odds ratio [OR], 1.36; 95% confidence interval [CI], 0.68-2.20; p = 0.028). The high CHA₂DS₂-VASc score showed a strong trend (OR, 1.71; 95% CI, 0.92-3.20; p = 0.089) in 1-year thromboembolic events.

Conclusion: No single risk factor or drug cessation strategy was attributed to adverse clinical events after GI bleeding. The risk of future thrombotic or rebleeding events should be individualized and controlled for based on a pre-existing stratification system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0269262PLOS
June 2022

Ultrafine Diesel Exhaust Particles Induce Apoptosis of Oligodendrocytes by Increasing Intracellular Reactive Oxygen Species through NADPH Oxidase Activation.

Antioxidants (Basel) 2022 May 23;11(5). Epub 2022 May 23.

Department of Anatomy, College of Medicine, Chungbuk National University, Cheongju 28644, Korea.

Diesel exhaust particles (DEPs) are a main contributor to air pollution. Ultrafine DEPs can cause neurodegenerative diseases by increasing intracellular reactive oxygen species (ROS). Compared with other cells in the brain, oligodendrocytes responsible for myelination are more susceptible to oxidative stress. However, the mechanisms underlying ROS generation in oligodendrocytes and the susceptibility of oligodendrocytes to ROS by ultrafine DEPs remain unclear. Herein, we examined the effects of excessive ROS generated by NOX2, an isoform of the NADPH oxidase family, after exposure to ultrafine DEPs (200 μg/mL) on the survival of two types of oligodendrocytes-oligodendrocyte precursor cells (OPCs) and mature oligodendrocytes (mOLs)--isolated from the brain of neonatal rats. In addition, mice were exposed to ultrafine DEP suspension (20 μL, 0.4 mg/mL) via the nasal route for 1 week, after which the expression of NOX2 and cleaved caspase-3 was examined in the white matter of the cerebellum. Exposure to DEPs significantly increased NOX2 expression and ROS generation in OPCs and mOLs. OPCs and mOLs clearly exhibited viability reduction, and a significant change in p53, Bax, Bcl-2, and cleaved caspase-3 expression, after DEP exposure. In contrast, treatment with berberine (BBR), an NOX2 inhibitor, significantly mitigated these effects. In mice exposed to DEP, the presence of NOX2-positive and cleaved caspase-3-positive oligodendrocytes was demonstrated in the cerebellar white matter; NOX2 and cleaved caspase-3 expression in the cerebellum lysates was significantly increased. BBR treatment returned expression of these proteins to control levels. These results demonstrate that the susceptibility of OPCs and mOLs to ultrafine DEPs is, at least in part, caused by excessive ROS produced by NOX2 and the sequential changes in the expression of p53, Bax, Bcl-2, and cleaved caspase-3. Overall, NOX2 inhibitor enhances the survival of two types of oligodendrocytes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/antiox11051031DOI Listing
May 2022

Predictors of end-of-life care stress, calling, and resilience on end-of-life care performance: a descriptive correlational study.

BMC Palliat Care 2022 May 17;21(1):77. Epub 2022 May 17.

Department of Nursing, Eulji University, Gyeonggi, Republic of Korea.

Background: Prolonging the end-of-life process means that the duration of health care work increases and the management of death is delegated to health care providers by patients' families. Thus, it is important to explore measures to enhance the quality of end-of-life care by identifying the predictors thereof. End-of-life care should be people-centred, relieving serious health-related suffering, be it physical, psychological, social, or spiritual. Nurses who provide end-of-life care usually spend the most time with dying patients, administering care to help patients who wish to die with dignity; therefore, end-of-life nursing care is highly significant.

Methods: This study was conducted on nurses of 500-bed or larger university hospitals in city D and province C in South Korea from 20 August to 10 September 2020 using a structured questionnaire. A total of 213 nurses with a minimum clinical career of one year and at least one EOLC experience participated. The final analysis sample consisted of 206 nurses. Descriptive analysis, Pearson's correlation coefficients, ANOVA, t-test, and multiple-regression analysis were used to analyse the data.

Results: This study found that end-of-life care performance was significantly positively correlated with end-of-life care stress [r = .253, p < .001], sense of calling [r = .424, p < .001], and resilience [r = .397, p < .001]. End-of-life care stress [β = .185, p = .003], sense of calling [β = .259, p < .001], resilience [β = .252, p < .001], and working in a hospice ward [β = .140, p = .041] or intensive care unit [β = .218, p = .008], as opposed to the emergency department, were identified as predictors of end-of-life care performance. These factors explained 28.3% of the variance in the end-of-life care performance in this study.

Conclusions: Boosting the sense of calling and resilience among nurses providing palliative care can improve overall end-of-life care performances. Subsequent studies should develop and evaluate interventions and programs that could improve these factors to ensure a positive change in health care and enhance the quality of end-of-life care in hospitals.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12904-022-00961-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110935PMC
May 2022

Complete Genome Sequence of sp. Strain CMM7, Isolated from a Marine Green Alga, (Schmidt) Silva.

Microbiol Resour Announc 2022 Jun 16;11(6):e0015322. Epub 2022 May 16.

Department of Oral Physiology, College of Dentistry, Dankook University, Cheonan, Choongnam, Republic of Korea.

Here, we report the genome sequence of sp. strain CMM7, which was isolated from a marine green alga, (Schmidt) Silva, in Jeju Island, South Korea. The genome is complete and consists of 4,421,981 bp, with a GC content of 37.5%, 3,942 predicted protein-coding sequences, and 49 RNA genes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/mra.00153-22DOI Listing
June 2022

Association between Physical Activity and Telomere Length in Women with Breast Cancer: A Systematic Review.

J Clin Med 2022 Apr 30;11(9). Epub 2022 Apr 30.

Department of Convergence Medicine, Wonju College of Medicine, Yonsei University, Wonju 26426, Korea.

The association between physical activity and telomere length (TL) has been continuously reported. However, the interplay of physical activity and TL among women with breast cancer has not been elucidated. Thus, the purpose of this systematic review was to synthesize the evidence for the association of physical activity with TL in women with breast cancer. Systematic searches were conducted to identify quantified studies using MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, Web of Science, and Clinical Trials.gov. Five studies were included in this systematic review. Three of the five studies reported that physical activity has a significant relationship in delaying TL shortening, but others observed no association between physical activity and TL in breast cancer survivors. Although the heterogeneous studies acted as limitations in drawing clear conclusions, physical activity strategies show encouraging impacts in delaying TL shortening. To understand the effects of physical activity on TL shortening in breast cancer survivors, further studies are needed considering the tissue site, treatments for breast cancer, DNA extraction methods, and tools for measuring physical activity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/jcm11092527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099544PMC
April 2022

Spatial epitranscriptomics reveals A-to-I editome specific to cancer stem cell microniches.

Nat Commun 2022 05 9;13(1):2540. Epub 2022 May 9.

Cancer Research Institute, Seoul National University, Seoul, 03080, Republic of Korea.

Epitranscriptomic features, such as single-base RNA editing, are sources of transcript diversity in cancer, but little is understood in terms of their spatial context in the tumour microenvironment. Here, we introduce spatial-histopathological examination-linked epitranscriptomics converged to transcriptomics with sequencing (Select-seq), which isolates regions of interest from immunofluorescence-stained tissue and obtains transcriptomic and epitranscriptomic data. With Select-seq, we analyse the cancer stem cell-like microniches in relation to the tumour microenvironment of triple-negative breast cancer patients. We identify alternative splice variants, perform complementarity-determining region analysis of infiltrating T cells and B cells, and assess adenosine-to-inosine base editing in tumour tissue sections. Especially, in triple-negative breast cancer microniches, adenosine-to-inosine editome specific to different microniche groups is identified.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-022-30299-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085828PMC
May 2022

Radiological clues for diagnosing intra-abdominal seminoma in undescended testis on computed tomography.

Radiol Case Rep 2022 Jun 26;17(6):2273-2276. Epub 2022 Apr 26.

Department of Radiology, Sanggye Paik Hospital Inje University College of Medicine, 1342 Dongil-ro, Nowon-gu, Seoul 01757, Repubilic of Korea.

We report a case of intra-abdominal seminoma in an undescended testis, focusing on the radiological clues for diagnosis on computed tomography. A 49-year-old man visited our hospital with a palpable abdominal mass and underwent abdominopelvic computed tomography. Computed tomography demonstrated an ovoid, mildly enhanced, well-defined mass measuring 21 × 16 × 9 cm in the small bowel mesentery mimicking a mass of mesenteric origin. However, a vascular structure was observed in the left posterior aspect of the mass. The vascular structure originated from the inferior posterior part of the mass and ran cranially. The artery subsequently united to the aorta, and the vein united to the left renal vein. We identified the artery and vein as the testicular artery and vein, respectively. We also noted the absence of a left spermatic cord in the left inguinal canal. Therefore, we concluded that the mass originated from the left undescended testis. The patient underwent surgery, and the mass was removed with the testicular vessels; the resected testicular vein was mostly filled with thrombus. On pathological examination, the mass was confirmed to be a seminoma in the undescended testis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.radcr.2022.03.071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062139PMC
June 2022

Relationship Between Breast and Axillary Pathologic Complete Response According to Clinical Nodal Stage: A Nationwide Study From Korean Breast Cancer Society.

J Breast Cancer 2022 Apr;25(2):94-105

Division of Breast Surgery, Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

Purpose: We evaluated the relationship between breast pathologic complete response (BpCR) and axillary pathologic complete response (ApCR) after neoadjuvant chemotherapy (NACT) according to nodal burden at presentation. As the indications for NACT have expanded, clinicians have started clinical trials for the omission of surgery from the treatment plan in patients with excellent responses to NACT. However, the appropriate indications for axillary surgery omission after excellent NACT response remain unclear.

Methods: Data were collected from patients in the Korean Breast Cancer Society Registry who underwent NACT followed by surgery between 2010 and 2020. We analyzed pathologic axillary nodal positivity after NACT according to BpCR stratified by tumor subtype in patients with cT1-3/N0-2 disease at diagnosis.

Results: A total of 6,597 patients were identified. Regarding cT stage, 528 (9.5%), 3,778 (67.8%), and 1,268 (22.7%) patients had cT1, cT2, and cT3 disease, respectively. Regarding cN stage, 1,539 (27.7%), 2,976 (53.6%), and 1,036 (18.7%) patients had cN0, cN1, and cN2 disease, respectively. BpCR occurred in 21.6% (n = 1,427) of patients, while ApCR and pathologic complete response (ypCR) occurred in 59.7% (n = 3,929) and ypCR 19.4% (n = 1,285) of patients, respectively. The distribution of biologic subtypes included 2,329 (39.3%) patients with hormone receptor (HR)-positive/human epidermal growth factor receptor 2 (HER2)-negative disease, 1,122 (18.9%) with HR-positive/HER2-positive disease, 405 (6.8%) with HR-negative/HER2-positive disease, and 2,072 (35.0%) with triple-negative breast cancer . Among the patients with BpCR, 89.6% (1,122/1,252) had ApCR. Of those with cN0 disease, most (99.0%, 301/304) showed ApCR. Among patients with cN1-2 disease, 86.6% (821/948) had ApCR.

Conclusion: BpCR was highly correlated with ApCR after NACT. In patients with cN0 and BpCR, the risk of missing axillary nodal metastasis was low after NACT. Further research on axillary surgery omission in patients with cN0 disease is needed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4048/jbc.2022.25.e17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065358PMC
April 2022

A novel HSP90 inhibitor SL-145 suppresses metastatic triple-negative breast cancer without triggering the heat shock response.

Oncogene 2022 06 2;41(23):3289-3297. Epub 2022 May 2.

Division of Medical Oncology, Department of Internal Medicine, Korea University College of Medicine, Korea University, Seoul, 02841, Republic of Korea.

Despite recent advances, there remains a significant unmet need for the development of new targeted therapies for triple-negative breast cancer (TNBC). Although the heat shock protein HSP90 is a promising target, previous inhibitors have had issues during development including undesirable induction of the heat shock response (HSR) and off-target effects leading to toxicity. SL-145 is a novel, rationally-designed C-terminal HSP90 inhibitor that induces apoptosis in TNBC cells via the suppression of oncogenic AKT, MEK/ERK, and JAK2/STAT3 signaling and does not trigger the HSR, in contrast to other inhibitors. In an orthotopic allograft model incorporating breast cancer stem cell-enriched TNBC tumors, SL-145 potently suppressed tumor growth, angiogenesis, and metastases concomitant with dysregulation of the JAK2/STAT3 signaling pathway. Our findings highlight the potential of SL-145 in suppressing metastatic TNBC independent of the HSR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41388-022-02269-yDOI Listing
June 2022

USP15 negatively regulates lung cancer progression through the TRAF6-BECN1 signaling axis for autophagy induction.

Cell Death Dis 2022 Apr 14;13(4):348. Epub 2022 Apr 14.

Department of Immunology, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea.

TNF receptor-associated factor 6 (TRAF6)-BECN1 signaling axis plays a pivotal role in autophagy induction through ubiquitination of BECN1, thereby inducing lung cancer migration and invasion in response to toll-like receptor 4 (TLR4) stimulation. Herein, we provide novel molecular and cellular mechanisms involved in the negative effect of ubiquitin-specific peptidase 15 (USP15) on lung cancer progression. Clinical data of the TCGA and primary non-small cell lung cancer (NSCLC) patients (n = 41) revealed that the expression of USP15 was significantly downregulated in lung cancer patients. Importantly, USP15-knockout (USP15KO) A549 and USP15KO H1299 lung cancer cells generated with CRISPR-Cas9 gene-editing technology showed increases in cancer migration and invasion with enhanced autophagy induction in response to TLR4 stimulation. In addition, biochemical studies revealed that USP15 interacted with BECN1, but not with TRAF6, and induced deubiquitination of BECN1, thereby attenuating autophagy induction. Notably, in primary NSCLC patients (n = 4) with low expression of USP15, 10 genes (CCNE1, MMP9, SFN, UBE2C, CCR2, FAM83A, ETV4, MYO7A, MMP11, and GSDMB) known to promote lung cancer progression were significantly upregulated, whereas 10 tumor suppressor genes (FMO2, ZBTB16, FCN3, TCF21, SFTPA1B, HPGD, SOSTDC1, TMEM100, GDF10, and WIF1) were downregulated, providing clinical relevance of the functional role of USP15 in lung cancer progression. Taken together, our data demonstrate that USP15 can negatively regulate the TRAF6-BECN1 signaling axis for autophagy induction. Thus, USP15 is implicated in lung cancer progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41419-022-04808-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9010460PMC
April 2022

PAX2, PAX8, and PR are correlated with ovarian seromucinous borderline tumor with endometriosis.

J Ovarian Res 2022 Apr 6;15(1):41. Epub 2022 Apr 6.

Department of Pathology, Yongin Severance Hospital, Yonsei University College of Medicine, 363, Dongbaekjukjeon-daero, Giheung-gu, Yongin-si, 16995, Gyeonggi-do, Korea.

Background: Ovarian "seromucinous carcinoma" has been recently removed in 2020 5 Edition of WHO classification of Female Genital Tumors and is considered as a subtype of endometrioid carcinoma with mucinous differentiation, while "seromucinous borderline tumor" remains and exists as a distinct entity. Both diseases may be considered as no more same lineage. However, ovarian seromucinous borderline tumor (SMBT) is also one of the endometriosis-related neoplasm of ovary similar to endometrioid tumor, featuring that about 50% of ovarian SMBTs combine endometriosis. The present study was aimed to investigate whether the ovarian SMBTs are different in clinical features and molecular patterns, according to the presence of combined endometriosis.

Results: There were no statistical differences in clinical findings between two groups. There was also no significant difference in pregnancy outcomes and recurrence between two groups. In immunohistochemical patterns, there was a statistically significant difference in PAX2 and PAX8 expression between in ovarian SMBT with or without endometriosis (P = 0.016, P < 0.001). Only a few cases of ovarian SMBT with endometriosis showed expression of PAX2 and conversely, most of the cases showed expression of PAX8. PR positivity was more prominent in ovarian SMBT with endometriosis than without endometriosis (P = 0.018), although there was no difference in positive ER expression. There were no statistical differences in WT1, CK20 and CDX2 expressions between two groups.

Conclusions: Ovarian SMBT with endometriosis did not clinically differ from that without endometriosis. However, the molecular patterns were different between two groups and ovarian SMBT with endometriosis is close to endometrioid tumor types unlike SMBT without endometriosis. Further, a direct comparison study between seromucinous borderline tumor and endometrioid borderline tumor is needed with a gene profiling study to prove their relationship.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13048-022-00975-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8985320PMC
April 2022

Additional Excision Biopsy in Patients With Atypical Ductal Hyperplasia at Ultrasound-guided Vacuum-assisted Breast Biopsy.

Anticancer Res 2022 04;42(4):2159-2165

The W Clinic, Seoul, Republic of Korea

Background/aim: We conducted this single-center, retrospective study to identify predictors of upgrading to malignancy and to discuss the necessity of additional excision biopsy in patients who were diagnosed with atypical ductal hyperplasia (ADH) at ultrasound (US)-guided vacuum-assisted breast biopsy (VABB) based on our 18-year, single-center experience.

Patients And Methods: The current study was conducted in a total of 12,160 patients who were evaluated at our medical institution during an 18-year period between January of 2003 and December of 2020. We included the patients who were diagnosed with ADH at US-guided VABB using the Mammotome (Devicor Medical Products, Inc., Cincinnati, OH, USA). We therefore included a total of 114 patients (n=114) with ADH in the current study.

Results: Of 114 eligible patients, 36 underwent additional excision biopsy and the remaining 78 did not. Of these 36 patients, 15 were found to have an upgrading to malignancy at a rate of upgrading of 41.7%. These include 7 cases (46.6%) of low-grade ductal carcinoma in situ (DCIS), 3 cases (20.0%) of intermediate grade DCIS, 1 case (6.7%) of microinvasive DCIS, 3 cases (20.0%) of multifocal lobular carcinoma in situ, and 1 case (6.7%) of mucinous carcinoma. Finally, only suspicious microcalcification on mammography was a significant predictor of upgrading to malignancy (p=0.023).

Conclusion: An additional excision biopsy is recommended to reduce the rate of upgrading to malignancy in patients who were diagnosed with ADH through a US-guided VABB.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21873/anticanres.15698DOI Listing
April 2022

Dual Beneficial Effects of α-Spinasterol Isolated from on Glucose Uptake in Skeletal Muscle Cells and Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells.

Plants (Basel) 2022 Feb 28;11(5). Epub 2022 Feb 28.

Cooperative-Center of Natural Product Central Bank for Biological Evaluation, College of Korean Medicine, Gachon University, Seongnam 13120, Korea.

Herein, we determined whether α-Spinasterol, a stigmastane-type phytosterol isolated from , potentially impacts glucose uptake and glucose-stimulated insulin secretion in skeletal muscle cells and pancreatic β-cells, respectively. We observed that and its fractions enhanced glucose uptake, with no toxic effects on C2C12 cells, with the -hexane fraction exhibiting the most potent effect. α-Spinasterol, isolated from the -hexane fraction, enhanced glucose uptake with no toxic effects on C2C12 cells. Additionally, α-Spinasterol increased the expression of associated proteins, including insulin receptor substrate-1, AMP-activated protein kinase, and glucose transporter type 4, as determined by Western blotting. Furthermore, α-Spinasterol enhanced insulin secretion in response to high glucose concentrations, with no toxic effects on INS-1 cells; this effect was superior to that demonstrated by gliclazide (positive control), commonly prescribed to treat type 2 diabetes (T2D). α-Spinasterol enhanced the expression of associated proteins, including insulin receptor substrate-2, peroxisome proliferator-activated receptor γ, and pancreatic and duodenal homeobox 1, as determined using Western blotting. The insulin secretory effect of α-Spinasterol was enhanced by a K channel blocker and L-type Ca channel agonist and was suppressed by a K channel activator and L-type Ca channel blocker. α-Spinasterol isolated from may improve hyperglycemia by improving glucose uptake into skeletal muscle cells and enhancing insulin secretion in pancreatic β-cells. Accordingly, α-Spinasterol could be a potential candidate for anti-T2D therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/plants11050658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912510PMC
February 2022

Adverse Pregnancy Outcomes and Maternal Chronic Diseases in the Future: A Cross-Sectional Study Using KoGES-HEXA Data.

J Clin Med 2022 Mar 7;11(5). Epub 2022 Mar 7.

Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea.

Adverse pregnancy outcomes (APOs) are associated with an increased risk of chronic diseases, including cardiovascular disease (CVD) and metabolic syndrome (MS), in the future. We designed a large-scale cohort study to evaluate the influence of APOs (preeclampsia, gestational diabetes mellitus (GDM), stillbirth, macrosomia, and low birth weight) on the incidence of chronic diseases, body measurements, and serum biochemistry in the future and investigate whether combinations of APOs had additive effects on chronic diseases. We used health examinee data from the Korean Genome and Epidemiology Study (KoGES-HEXA) and extracted data of parous women ( = 30,174; mean age, 53.02 years) for the analysis. Women with APOs were more frequently diagnosed with chronic diseases and had a family history of chronic diseases compared with women without APOs. Composite APOs were associated with an increased risk of hypertension, diabetes mellitus, hyperlipidemia, angina pectoris, stroke, and MS (adjusted odds ratio: 1.093, 1.379, 1.269, 1.351, 1.414, and 1.104, respectively) after adjustment for family history and social behaviors. Preeclampsia and GDM were associated with an increased risk of some chronic diseases; however, the combination of preeclampsia and GDM did not have an additive effect on the risk. APOs moderately influenced the future development of maternal CVD and metabolic derangements, independent of family history and social behaviors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/jcm11051457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911450PMC
March 2022

Correction to: Stable Zn Metal Anodes with Limited Zn-Doping in MgF Interphase for Fast and Uniformly Ionic Flux.

Nanomicro Lett 2022 Mar 4;14(1):70. Epub 2022 Mar 4.

Energy Storage Research Center, Korea Institute of Science and Technology, Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul, 02792, Republic of Korea.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s40820-022-00814-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894508PMC
March 2022

The investigation of the relation between expansion strategy and outcomes of two-stage expander-implant breast reconstruction.

Gland Surg 2022 Jan;11(1):1-11

Department of Surgery, Ajou University School of Medicine, Suwon, Korea.

Background: Numerous risk factors for the complications of two-stage, immediate implant-based breast reconstruction have been identified, although few studies have directly examined the impact of breast size and expansion protocols on the surgical outcomes of breast reconstruction. This study aimed to evaluate the impact of breast size, expansion velocity, and volume-related variables on postoperative complications of breast reconstruction.

Methods: The cohort involved patients who underwent immediate breast expander reconstruction at a single center between 2017 and 2019. The breast size was classified into three categories according to the weight of the mastectomy specimen as small (<300 g), medium (≥300 g, ≤500 g), or large (>500 g). Multifactorial logistic regressions were used to assess the impact of variables, and receiver operating characteristic (ROC) curve analysis was used to determine the optimal cut-off value for predicting the complication event.

Results: Of the 174 breasts (168 patients), 51 (29.3%), 66 (37.9%), and 57 (32.6%) breasts were classified as small, medium, and large, respectively. The rate of infection (P=0.014) and expander/implant failure (P=0.007) significantly differed according to breast size, with the rate being the highest in large breasts. Multivariate logistic regression analysis showed that body mass index (BMI) [odds ratio (OR): 1.25; P=0.003], nipple-sparing mastectomy (OR: 2.82; P=0.036), sentinel biopsy (OR: 5.10; P=0.016), final expansion volume (OR: 0.99; P=0.022), and expansion velocity (OR: 0.703; P=0.024) were significant independent predictors of any complication. In the ROC analysis, breast weight >696 g could predict the possibility of revision surgery, with a sensitivity of 42.9% and specificity of 81.8%.

Conclusions: The final expansion volume and expansion velocity have a significant negative relationship with overall complications in breast reconstruction. A standard expansion protocol needs to be established to ensure the success of two-stage breast reconstruction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/gs-21-515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8825526PMC
January 2022

Biomarker LEPRE1 induces pelitinib-specific drug responsiveness by regulating ABCG2 expression and tumor transition states in human leukemia and lung cancer.

Sci Rep 2022 02 21;12(1):2928. Epub 2022 Feb 21.

Genome Data Integration Centre, Syntekabio Inc., Daejeon, 34025, Republic of Korea.

Biomarkers for treatment sensitivity or drug resistance used in precision medicine include prognostic and predictive molecules, critical factors in selecting appropriate treatment protocols and improving survival rates. However, identification of accurate biomarkers remain challenging due to the high risk of false-positive findings and lack of functional validation results for each biomarker. Here, we discovered a mechanical correlation between leucine proline-enriched proteoglycan 1 (LEPRE1) and pelitinib drug sensitivity using in silico statistical methods and confirmed the correlation in acute myeloid leukemia (AML) and A549 lung cancer cells. We determined that high LEPRE1 levels induce protein kinase B activation, overexpression of ATP-binding cassette superfamily G member 2 (ABCG2) and E-cadherin, and cell colonization, resulting in a cancer stem cell-like phenotype. Sensitivity to pelitinib increases in LEPRE1-overexpressing cells due to the reversing effect of ABCG2 upregulation. LEPRE1 silencing induces pelitinib resistance and promotes epithelial-to-mesenchymal transition through actin rearrangement via a series of Src/ERK/cofilin cascades. The in silico results identified a mechanistic relationship between LEPRE1 and pelitinib drug sensitivity, confirmed in two cancer types. This study demonstrates the potential of LEPRE1 as a biomarker in cancer through in-silico prediction and in vitro experiments supporting the clinical development of personalized medicine strategies based on bioinformatics findings.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-022-06621-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861100PMC
February 2022

Relationship between type D personality, symptoms, cancer stigma, and quality of life among patients with lung cancer.

Eur J Oncol Nurs 2022 Apr 1;57:102098. Epub 2022 Feb 1.

Department of Internal Medicine and Research Center for Pulmonary Disorders, Jeonbuk National University Medical School, Jeonju, South Korea. Electronic address:

Purpose: This study aimed to investigate the influence of type D personality on quality of life in patients with lung cancer.

Methods: A correlational, cross-sectional research design was used. A convenience sample of 136 patients with lung cancer were recruited from an outpatient pulmonology clinic. Data collection was performed using a structured questionnaire between July and August 2019. Data analyses included descriptive statistics, an independent t-test, a one-way ANOVA, the χ test, an ANCOVA, Pearson's correlation coefficients, and hierarchical regression analysis, which were performed using the SPSS WIN 25.0 program.

Results: Type D personality was identified in 18.4% of the participants. Patients with type D personality had poorer quality of life and experienced more cancer stigma and more severe symptoms. Type D personality had the strongest association with quality of life among patients with lung cancer, followed by cancer stigma and symptoms. Poor quality of life was associated with non-married status and higher Eastern Cooperative Oncology Group grade.

Conclusions: Type D personality, stigma, symptoms, and demographic and clinical factors should be considered when assessing quality of life in patients with lung cancer. Interventions that reflect these factors, including type D personality, may help enhance quality of life for patients with lung cancer in oncology nursing practice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejon.2022.102098DOI Listing
April 2022

Positron emission tomography imaging of system xc in immune cells for assessment of disease activity in mice and patients with inflammatory bowel disease.

J Nucl Med 2022 Jan 27. Epub 2022 Jan 27.

Asan Medical Center, University of Ulsan College of Medicine, Korea, Republic of.

We aimed to explore whether the imaging of antiporter system xC of immune cells with (4)-4-(3-[F-fluoropropyl)-L-glutamate (F-FSPG) positron emission tomography (PET) can assess inflammatory bowel disease (IBD) activity in murine models and patients (NCT03546868). : F-FSPG PET imaging was performed to assess IBD activity in mice with dextran sulfate sodium-induced and adoptive T-cell transfer-induced IBD and a cohort of 20 patients at a tertiary care center in South Korea. Immunohistochemical analysis of system xC and cell surface markers was also studied. : Mice with experimental IBD showed increased intestinal F-FSPG uptake and xCT expression in CD11c+, F4/80+, and CD3+ cells in the lamina propria, increases positively associated with clinical and pathological disease activity. F-FSPG PET studies in patients, most of whom were clinically in remission or had mildly active IBD, showed that PET imaging was sufficiently accurate in diagnosing endoscopically active IBD and remission in patients and bowel segments. F-FSPG PET correctly identified all nine patients with superficial or deep ulcers. Quantitative intestinal F-FSPG uptake was strongly associated with endoscopic indices of IBD activity. The number of CD68+xCT+ and CD3+xCT+ cells in 22 bowel segments from patients with ulcerative colitis and the number of CD68+xCT+ cells in seven bowel segments from patients with Crohn's disease showed a significant positive association with endoscopic indices of IBD activity. : The assessment of system xC in immune cells may provide diagnostic information on the immune responses responsible for chronic active inflammation in IBD. F-FSPG PET imaging of system xC activity may noninvasively assess the IBD activity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2967/jnumed.121.263289DOI Listing
January 2022

Effects of diesel exhaust particles and urban particles on brain endothelial cells.

Toxicol Res 2022 Jan 13;38(1):91-98. Epub 2021 Oct 13.

Department of Preventive Medicine, College of Medicine, Chungbuk National University, Cheongju, 28644 Republic of Korea.

Exposure to diesel exhaust particles (DEPs) and urban particles (UPs) increases the incidence of degenerative brain diseases as well as respiratory diseases. However, there is limited evidence on the mechanism of neurotoxicity on exposure to these particles. In the present study, the damage to blood-brain barrier (BBB) function by DEP or UP exposure was evaluated in bEnd.3 cells, which are derived from the brain tissue of Balb/c mice. It was demonstrated that DEP and UP exposure may induce oxidative stress via increasing reactive oxygen species (ROS) and decreasing total antioxidant capacity (TAC) level in bEnd.3 cells. In addition, cells exposed to DEP and UP demonstrated a resistance value of about 50% each compared to the value noted prior to exposure; additionally, Claudin-5 and ZO-1 expression levels were significantly decreased compared to the corresponding levels in the control. It was inferred that DEP or UP exposure diminishes the expression of tight junction proteins in endothelial cells through ROS generation, thereby enhancing endothelial membrane permeability. This study showed that DEPs or UPs induced cell permeability and oxidative stress by increasing ROS generation in bEnd.3 cells. This suggests the possibility that exposure to DEPs or UPs may compromise the integrity of the BBB and induce adverse effects in the CNS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s43188-021-00110-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748579PMC
January 2022

Stable Zn Metal Anodes with Limited Zn-Doping in MgF Interphase for Fast and Uniformly Ionic Flux.

Nanomicro Lett 2022 Jan 22;14(1):46. Epub 2022 Jan 22.

Energy Storage Research Center, Korea Institute of Science and Technology, Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul, 02792, Republic of Korea.

The practical applications of aqueous Zn metal batteries are currently restricted by the inherent drawbacks of Zn such as the hydrogen evolution reaction, sluggish kinetics, and dendrite formation. To address these problems, herein, a limitedly Zn-doped MgF interphase comprising an upper region of pure, porous MgF and a lower region of gradient Zn-doped MgF is achieved via radio frequency sputtering technique. The porous MgF region is a polar insulator whose high corrosion resistance facilitates the de-solvation of the solvated Zn ions and suppression of hydrogen evolution, resulting in Zn metal electrodes with a low interfacial resistance. The Zn-doped MgF region facilitates fast transfer kinetics and homogeneous deposition of Zn ions owing to the interfacial polarization between the Zn dopant and MgF matrix, and the high concentration of the Zn dopant on the surface of the metal substrate as fine nuclei. Consequently, a symmetric cell incorporating the proposed Zn metal exhibits low overpotentials of ~ 27.2 and ~ 99.7 mV without Zn dendrites over 250 to 8000 cycles at current densities of 1.0 and 10.0 mA cm, respectively. The developed Zn/MnO full cell exhibits superior capacity retentions of 97.5% and 84.0% with average Coulombic efficiencies of 99.96% after 1000 and 3000 cycles, respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s40820-021-00788-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783935PMC
January 2022

The Influence of Parent Media Use, Parent Attitude on Media, and Parenting Style on Children's Media Use.

Children (Basel) 2022 Jan 1;9(1). Epub 2022 Jan 1.

Communication & Media Research Center, Ewha Womans University, Seoul 03760, Korea.

Parents play a vital role in mediating children's media use, especially at a young age. We examined the link between the media use of younger children and the media use, attitude toward media, and parenting styles of parents. One thousand and twenty parents of children between 4 and 6 years of age completed a questionnaire on their media use, positive and negative attitudes on media, parenting styles, and the media use of their children. Multigroup structural equation modeling was used to analyze the data. The results showed that there was a significant positive relation between the parent's media time and the child's daytime and nighttime media use. Additionally, the parent's positive attitude toward media use was positively related to the child's daytime media use, but not the child's nighttime media use, while the parent's negative attitude toward media was not associated with the child's daytime and nighttime media use. Further, among the seven parenting styles, material rewards and autonomy were positively associated with the child's daytime media use. Discipline was negatively related to the child's nighttime media use, whereas material rewards were positively associated with the child's nighttime media use. Collectively, the parent's positive attitude toward media use was the strongest predictor of the child's daytime media use, and material rewards were the strongest predictor of the child's nighttime media use. These results can be of significant use to inform policymakers, researchers, and parents regarding the development of parental guidelines on children's media use.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/children9010037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8774813PMC
January 2022

Enantiomer-dependent immunological response to chiral nanoparticles.

Nature 2022 01 19;601(7893):366-373. Epub 2022 Jan 19.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.

Chirality is a unifying structural metric of biological and abiological forms of matter. Over the past decade, considerable clarity has been achieved in understanding the chemistry and physics of chiral inorganic nanoparticles; however, little is known about their effects on complex biochemical networks. Intermolecular interactions of biological molecules and inorganic nanoparticles show some commonalities, but these structures differ in scale, in geometry and in the dynamics of chiral shapes, which can both impede and strengthen their mirror-asymmetric complexes. Here we show that achiral and left- and right-handed gold biomimetic nanoparticles show different in vitro and in vivo immune responses. We use irradiation with circularly polarized light (CPL) to synthesize nanoparticles with controllable nanometre-scale chirality and optical anisotropy factors (g-factors) of up to 0.4. We find that binding of nanoparticles to two proteins from the family of adhesion G-protein-coupled receptors (AGPCRs)-namely cluster-of-differentiation 97 (CD97) and epidermal-growth-factor-like-module receptor 1 (EMR1)-results in the opening of mechanosensitive potassium-efflux channels, the production of immune signalling complexes known as inflammasomes, and the maturation of mouse bone-marrow-derived dendritic cells. Both in vivo and in vitro immune responses depend monotonically on the g-factors of the nanoparticles, indicating that nanoscale chirality can be used to regulate the maturation of immune cells. Finally, left-handed nanoparticles show substantially higher (1,258-fold) efficiency compared with their right-handed counterparts as adjuvants for vaccination against the H9N2 influenza virus, opening a path to the use of nanoscale chirality in immunology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41586-021-04243-2DOI Listing
January 2022

Multifactorial engineering of biomimetic membranes for batteries with multiple high-performance parameters.

Nat Commun 2022 01 12;13(1):278. Epub 2022 Jan 12.

Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.

Lithium-sulfur (Li-S) batteries have a high specific capacity, but lithium polysulfide (LPS) diffusion and lithium dendrite growth drastically reduce their cycle life. High discharge rates also necessitate their resilience to high temperature. Here we show that biomimetic self-assembled membranes from aramid nanofibers (ANFs) address these challenges. Replicating the fibrous structure of cartilage, multifactorial engineering of ion-selective mechanical, and thermal properties becomes possible. LPS adsorption on ANF surface creates a layer of negative charge on nanoscale pores blocking LPS transport. The batteries using cartilage-like bioinspired ANF membranes exhibited a close-to-theoretical-maximum capacity of 1268 mAh g, up to 3500+ cycle life, and up to 3C discharge rates. Essential for safety, the high thermal resilience of ANFs enables operation at temperatures up to 80 °C. The simplicity of synthesis and recyclability of ANFs open the door for engineering high-performance materials for numerous energy technologies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-27861-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755825PMC
January 2022
-->