Publications by authors named "Ji Yoon Kim"

145 Publications

Kinetics of the neutrophil-lymphocyte ratio during PD-1 inhibition as a prognostic factor in advanced hepatocellular carcinoma.

Liver Int 2021 May 9. Epub 2021 May 9.

Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Liver Center, Seoul, Republic of Korea.

Background & Aims: Programmed death-1 (PD-1) inhibitors have improved survival outcomes and produced durable responses in advanced hepatocellular carcinoma (HCC) for some patients. Here, we evaluated the relationship between the baseline and kinetics of the neutrophil-lymphocyte ratio (NLR) and clinical outcomes in nivolumab-treated HCC patients.

Methods: All consecutive HCC patients treated with nivolumab between July 2017 and June 2020 were screened for the eligibility. The NLRs were calculated before and at 2, 4, and 6 weeks after treatment. Survival outcomes were compared based on the baseline and kinetics of NLR. We additionally analyzed the association of the baseline and dynamic changes in the NLR with hyperprogression (HPD).

Results: Among the 194 included cases, most patients were male (82.0%) and had a Child-Pugh class A disease (70.6%). Patients with a baseline NLR ≥3 (hazard ratio [HR] 2.46; 95% CI 1.63-3.71) had a poorer overall survival than patients with baseline NLR <3. During the treatment, the NLR increased rapidly in patients developing HPD and only a ΔNLR at 4 weeks was predictive of HPD. The risk of HPD increased by 20% for every 20% increase in the ΔNLR at 4 weeks. Accordingly, an NLR increase at 4 weeks (HR 1.79; 95% CI 1.19-2.68) was associated with an increased risk of death, especially among patients with a baseline NLR ≥3.

Conclusions: The baseline and on-treatment kinetics for the NLR are effective prognostic indicators in nivolumab-treated patients with HCC. This may help to guide patient selection and on-treatment strategies for immunotherapies in advanced HCC.
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http://dx.doi.org/10.1111/liv.14932DOI Listing
May 2021

Clinical Characteristics and Treatment Outcomes of Childhood Acute Promyelocytic Leukemia in Korea: a Nationwide Multicenter Retrospective Study by Korean Pediatric Oncology Study Group.

Cancer Res Treat 2021 Apr 20. Epub 2021 Apr 20.

Department of Pediatrics, Kosin University of Medicine, Busan, Korea.

Purpose: Acute promyelocytic leukemia (APL) is a rare disease in children and there are some different characteristics between children and adult. We aimed to evaluate incidence, clinical characteristics and treatment outcomes of pediatric APL in Korea.

Materials And Methods: Seventy-nine pediatric APL patients diagnosed from January 2009 to December 2016 in 16 tertiary medical centers in Korea were reviewed retrospectively.

Results: Of 801 acute myeloid leukemia(AML) children, 79 (9.9%) were diagnosed with APL. The median age at diagnosis was 10.6 years (range, 1.3-18.0). Male and female ratio was 1:0.93. Thirty patients (38.0%) had WBC count greater than 10x109/L at diagnosis. All patients received induction therapy consisting of all-trans retinoic acid (ATRA) and chemotherapy. Five patients (6.6%) died during induction chemotherapy and 66 (86.8%) patients achieved complete remission (CR) after induction chemotherapy. The causes of death were 3 intracranial hemorrhage (ICH), 1 cerebral infarction, and 1 sepsis. Five patients (7.1%) suffered a relapse during or after maintenance chemotherapy. The estimated 4-year event-free survival rate (EFS) and overall survival rates (OS) were 82.1 ± 4.4%, 89.7 ± 5.1% respectively. The 4-yr OS was significantly higher in patients with initial WBC <10x109/L than in those with initial WBC ≥10x109/L (p=0.02).

Conclusion: This study showed that the CR rates and survival outcomes in Korean pediatric APL patients were relatively good. The initial WBC count was the most important prognostic factor and most causes of death were related to serious bleeding in the early stage of treatment.
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http://dx.doi.org/10.4143/crt.2021.313DOI Listing
April 2021

Responses of phytoplankton community structure and association to variability in environmental drivers in a tropical coastal lagoon.

Sci Total Environ 2021 Apr 1;783:146873. Epub 2021 Apr 1.

Wetland Research and Training Centre, Chilika Development Authority, Balugaon 752030, Odisha, India. Electronic address:

Spatial and seasonal heterogeneity in phytoplankton communities are governed by many biotic and abiotic drivers. However, the identification of long-term spatial and temporal trends in abiotic drivers, and their interdependencies with the phytoplankton communities' structure is understudied in tropical brackish coastal lagoons. We examined phytoplankton communities' spatiotemporal dynamics from a 5-year dataset (n = 780) collected from 13 sampling stations in Chilika Lagoon, India, where the salinity gradient defined the spatial patterns in environmental variables. Generalized additive models showed a declining trend in phytoplankton biomass, pH, and dissolved PO in the lagoon. Hierarchical modelling of species communities revealed that salinity (44.48 ± 28.19%), water temperature (4.37 ± 5.65%), and season (4.27 ± 0.96%) accounted for maximum variation in the phytoplankton composition. Bacillariophyta (Indicator Value (IV): 0.74) and Dinophyta (IV: 0.72) emerged as top indicators for polyhaline regime whereas, Cyanophyta (IV: 0.81), Euglenophyta (IV: 0.79), and Chlorophyta (IV: 0.75) were strong indicators for oligohaline regime. The responses of Dinophyta and Chrysophyta to environmental drivers were much more complex as random effects accounted for ~70-75% variation in their abundances. Prorocentrum minimum (IV: 0.52), Gonyaulax sp. (IV: 0.52), and Alexandrium sp. (IV: 0.51) were potential indicators of P-limitation. Diploneis weissflogii (IV: 0.43), a marine diatom, emerged as a potential indicator of N-limitation. Hierarchical modelling revealed the positive association between Cyanophyta, Chlorophyta, and Euglenophyta whereas, Dinophyta and Chrysophyta showed a negative association with Cyanophyta, Chlorophyta, and Euglenophyta. Landsat 8-Operational Land Imager satellite models predicted the highest and lowest Cyanophyta abundances in northern and southern sectors, respectively, which were in accordance with the near-coincident field-based measurements from the lagoon. This study highlighted the dynamics of phytoplankton communities and their relationships with environmental drivers by separating the signals of habitat filtering and biotic interactions in a monsoon-regulated tropical coastal lagoon.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146873DOI Listing
April 2021

Korean Red ginseng prevents endothelial senescence by downregulating the HO-1/NF-κB/miRNA-155-5p/eNOS pathway.

J Ginseng Res 2021 Mar 14;45(2):344-353. Epub 2020 Aug 14.

Department of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 24341, South Korea.

Background: Korean Red ginseng extract (KRGE) has beneficial effects on the cardiovascular system by improving endothelial cell function. However, its pharmacological effect on endothelial cell senescence has not been clearly elucidated. Therefore, we examined the effect and molecular mechanism of KRGE on the senescence of human umbilical vein endothelial cells (HUVECs).

Methods: HUVECs were grown in normal or KRGE-supplemented medium. Furthermore, they were transfected with heme oxygenase-1 (HO-1) gene or treated with its inhibitor, a NF-B inhibitor, and a miR-155-5p mimic or inhibitor. Senescence-associated characteristics of endothelial cells were determined by biochemical and immunohistochemical analyses.

Results: Treatment of HUVECs with KRGE resulted in delayed onset and progression of senescence-associated characteristics, such as increased lysosomal acidic β-galactosidase and decreased telomerase activity, angiogenic dysfunction, and abnormal cell morphology. KRGE preserved the levels of anti-senescent factors, such as eNOS-derived NO, MnSOD, and cyclins D and A: however, it decreased the levels of senescence-promoting factors, such as ROS, activated NF-B, endothelial cell inflammation, and p21 expression. The beneficial effects of KRGE were due to the induction of HO-1 and the inhibition of NF-B-dependent biogenesis of miR-155-5p that led to the downregulation of eNOS. Moreover, treatment with inhibitors of HO-1, NF-B, and miR-155-5p abolished the anti-senescence effects of KRGE.

Conclusion: KRGE delayed or prevented HUVEC senescence through a signaling cascade involving the induction of HO-1, the inhibition of NF-B-dependent miR-155-5p biogenesis, and the maintenance of the eNOS/NO axis activity, suggesting that it may protect against vascular diseases associated with endothelial senescence.
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http://dx.doi.org/10.1016/j.jgr.2020.08.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020293PMC
March 2021

Lipid Emulsion Enhances Vasoconstriction Induced by Dexmedetomidine in the Isolated Endothelium-Intact Aorta.

Int J Mol Sci 2021 Mar 24;22(7). Epub 2021 Mar 24.

Institute of Health Sciences, Gyeongsang National University, Jinju-si 52727, Korea.

This study aimed to examine the effect of lipid emulsion (LE) on the vasoconstriction induced by dexmedetomidine (DMT) in the isolated rat aorta and elucidate the associated cellular mechanism. The effect of LE, N-nitro-L-arginine methyl ester (L-NAME), and methyl-β-cyclodextrin (MβCD) on the DMT-induced contraction was examined. We investigated the effect of LE on the DMT-induced cyclic guanosine monophosphate (cGMP) formation and DMT concentration. The effect of DMT, LE, 4-Amino-3-(4-chlorophenyl)-1-(t-butyl)-1H-pyrazolo[3,4-d]pyrimidine,4-Amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2), and rauwolscine on the phosphorylation of endothelial nitric oxide synthase (eNOS), caveolin-1, and Src kinase was examined in the human umbilical vein endothelial cells. L-NAME, MβCD, and LE (1%, standardized mean difference (SMD): 2.517) increased the DMT-induced contraction in the endothelium-intact rat aorta. LE (1%) decreased the DMT (10 M) concentration (SMD: -6.795) and DMT-induced cGMP formation (SMD: -2.132). LE (1%) reversed the DMT-induced eNOS (Ser1177 and Thr496) phosphorylation. PP2 inhibited caveolin-1 and eNOS phosphorylation induced by DMT. DMT increased the Src kinase phosphorylation. Thus, LE (1%) enhanced the DMT-induced contraction by inhibition of NO synthesis, which may be caused by the decreased DMT concentration. DMT-induced NO synthesis may be caused by the increased eNOS (Ser1177) phosphorylation and decreased eNOS (Thr495) phosphorylation potentially mediated by Src kinase-induced caveolin-1 phosphorylation.
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http://dx.doi.org/10.3390/ijms22073309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038020PMC
March 2021

Long-Term Interannual and Seasonal Links between the Nutrient Regime, Sestonic Chlorophyll and Dominant Bluegreen Algae under the Varying Intensity of Monsoon Precipitation in a Drinking Water Reservoir.

Int J Environ Res Public Health 2021 03 11;18(6). Epub 2021 Mar 11.

Department of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Korea.

Long-term variations in reservoir water chemistry could provide essential data in making sustainable water quality management decisions. Here, we analyzed the spatiotemporal variabilities of nutrients, sestonic chlorophyll-a (CHL-a), nutrient enrichment, dominant algal species, and overall chemical water health of the third-largest drinking water reservoir in South Korea during 2000-2020. Our results distinctly explained the strong influence of monsoon rainfall on spatial and annual water chemistry variations. We observed a consistent increase in the chemical oxygen demand alluding to organic matter pollutants, while a steady declining trend in the sestonic CHL-a. The long-term total phosphorus (TP) level showed a steady reduction from the riverine zone to the lacustrine area. However, a higher total coliform bacteria (TCB) was observed at the water intake tower sites. TP displayed a strong link to algal CHL-a and ambient nitrogen phosphorus ratios, suggesting a robust phosphorus-limitation state. The severe phosphorus-limitation was also corroborated by the findings of trophic state index deviation. The high and low flow dynamics exhibited the strong influence of intensive rainfall carrying many nutrients and sediments and flushing out the sestonic CHL-a. Successive eutrophic conditions prevailed along with dominating blue-green algae species (Microcystis and Anabaena). We observed a strong positive correlation (r = 0.62) between water temperature and CHL-a and between total suspended solids and TP (r = 0.65). The multi-metric water pollution index characterized the overall water quality as 'good' at all the study sites. In conclusion, the long-term spatiotemporal variabilities of the ecological functions based on the nutrient-CHL-a empirical models are regulated mainly by the intensive monsoon precipitation. The drinking water could become hazardous under the recurrent eutrophication events and chemical degradations due to uncontrolled and untreated inflow of sewage and wastewater treatment plant effluents. Therefore, we strongly advocate stringent criteria to mitigate phosphorus and organic pollutant influx for sustainable management of Daecheong Reservoir.
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http://dx.doi.org/10.3390/ijerph18062871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7998934PMC
March 2021

Oscillatory shear stress promotes angiogenic effects in arteriovenous malformations endothelial cells.

Mol Med 2021 03 31;27(1):31. Epub 2021 Mar 31.

Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, 130 Dongdeok-ro, Jung-gu, Daegu, 41944, Korea.

Background: Vascular endothelial cells (ECs) are subject to continuous shear stress due to blood circulation. Mechanical stress due to high shear flow can also cause arteriovenous malformation (AVM) when ECs respond hyper-sensitively to shear flow. This study was conducted to test the hypothesis that angiogenesis could be promoted in response to mechanical stress via regulation of pro-angiogenic factors in AVM cells.

Methods: ECs were extracted from the tissue samples from six AVM patients and six normal patients. Shear stress at 7 dynes/cm2 were applied for 24 h. Before and after application of shear stress to each group, RT-PCR was performed to access the expression levels of angiopoietin2(AGP2), aquaporin1(AQP1) and TGFβR1. Immunofluorescences was also performed to evaluate the level of protein expressions.

Results: In both normal and AVM tissues, AGP2 and TGFβR1 under the shear stress showed increased expression in the ECs compared to the non-sheared samples. When AVMs and normal arterial vasculature were compared, the expression levels of both AGP2 and TGFβR1 in AVMs were higher when compared to normal arterial vasculature with or without shear stress. Immunofluorescence-based protein analysis also confirmed shear-induced AGP2 and TGFβR1 in both samples of normal and AVM patients.

Conclusions: AVMs exhibited higher sensitivity to shear stress by producing higher expressions of some marked genes and proteins that regulate the endothelial functions upon exposure to shear stress. While the physiological mechanism for AVMs remain elusive, our study shows the plausibility of physical stress imposed by the shearing flow can cause the occurrence of AVMs.
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http://dx.doi.org/10.1186/s10020-021-00291-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011119PMC
March 2021

Current site planning of medium to large solar power systems accelerates the loss of the remaining semi-natural and agricultural habitats.

Sci Total Environ 2021 Mar 16;779:146475. Epub 2021 Mar 16.

Center for Climate Change Adaptation, National Institute for Environmental Studies, Tsukuba 305-8506, Japan.

The global transition to renewable energy sources has accelerated to mitigate the effects of global climate change. Sudden increases in solar power facilities have caused the physical destruction of wildlife habitats, thereby resulting in the decline of biodiversity and ecosystem functions. However, previous assessments have been based on the environmental impact of large solar photovoltaics (PVs). The impact of medium-sized PV facilities (0.5-10 MW), which can alter small habitat patches through the accumulation of installations has not been assessed. Here, we quantified the amount of habitat loss directly related to the construction of PV facilities with different size classes and estimated their siting attributes using construction patterns in Japan and South Korea. We identified that a comparable amount of natural and semi-natural habitats were lost due to the recent installation of medium solar facilities (approximately 66.36 and 85.73% of the overall loss in Japan and South Korea, respectively). Compared to large solar PVs, medium PV installations resulted in a higher area loss of semi-natural habitats, including secondary/planted forests, secondary/artificial grasslands, and agricultural lands. The siting attributes of medium and large solar PV facilities indicated a preference for cost-based site selection rather than prioritizing habitat protection for biodiversity conservation. Moreover, even conservation areas were developed when economic and topological conditions were suitable for energy production. Our simulations indicate that increasing the construction of PVs in urban areas could help reduce the loss of natural and semi-natural habitats. To improve the renewable energy share while mitigating the impacts on biodiversity, our results stress the need for a proactive assessment to enforce sustainable site-selection criteria for solar PVs in renewable energy initiatives. The revised criteria should consider the cumulative impacts of varied size classes of solar power facilities, including medium PVs, and the diverse aspects of the ecological value of natural habitats.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146475DOI Listing
March 2021

Assessment of potential infectivity of human norovirus in the traditional Korean salted clam product "Jogaejeotgal" by floating electrode-dielectric barrier discharge plasma.

Food Res Int 2021 Mar 18;141:110107. Epub 2021 Jan 18.

Department of Seafood and Aquaculture Science/Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Republic of Korea. Electronic address:

This study investigated the antiviral effects of floating electrode-dielectric barrier discharge (FE-DBD) plasma treatment (1.1 kV, 43 kHz, N 1.5 m/s, 5-30 min) against human norovirus (HuNoV) GII.4 in Jogaejeotgal Infectivity was assessed using real-time quantitative-PCR (RT-qPCR) following treatment of samples with propidium monoazide (PMA) and sodium lauroyl sarcosinate (Sarkosyl). This study also investigated the effects of FE-DBD plasma treatment on Jogaejeotgal quality (assessed using pH value and Hunter colors). Following inoculation, the average titers of HuNoV GII.4 in Jogaejeotgal significantly (P < 0.05) decreased with increases in the FE-DBD plasma treatment time in both the non-PMA-treated and PMA + Sarkosyl-treated samples; in the non-PMA and PMA + Sarkosyl treated Jogaejeotgal, HuNoV GII.4 titers (log copy number/µL) were to: 3.16 and 2.95 (5 min), 2.90 and 2.48 (10 min), 2.82 and 2.40 (15 min), 2.58 and 2.26 (20 min), 2.48 and 2.06 (25 min), and 2.23 and 1.91 (30 min), respectively. The average titers of HuNoV demonstrated significant (P < 0.05) reductions of 0.35 log (55.3%) in PMA + Sarkosyl-treated samples compared with the non-PMA treated samples following exposure to 5-30 min of FE-DBD plasma. Reductions of >1-log for HuNoV in PMA + Sarkosyl- treated Jogaejeotgal required treatments of FE-DBD of 5-30 min. Using the first order kinetic model (R = 0.95), GII.4 decimal reduction time (D-value) resulting from FE-DBD plasma was 23.75 min. The pH and Hunter colors ("L", "a", and "b") were not significantly different (P > 0.05) between the untreated and FE-DBD plasma-treated Jogaejeotgal. Based on these results, the PMA + Sarkosyl/RT-qPCR method could be assessing HuNoV viability following 5-30 min treatment of FE-DBD plasma. Furthermore, may be an optimal treatment for Jogaejeotgal without altering the food quality (color and pH).
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http://dx.doi.org/10.1016/j.foodres.2021.110107DOI Listing
March 2021

Incidence and prevalence of immune thrombocytopenia under the copayment waiver policy for pediatric patients in Korea: Data from the National Health Claims Database.

Lupus 2021 Apr 16;30(4):655-660. Epub 2021 Feb 16.

Department of Pediatrics, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, Republic of Korea.

The purpose of this study was to investigate the epidemiology of immune thrombocytopenia (ITP) under the copayment waiver policy for pediatric patients in Korea. The data were collected from the National Health Insurance Claims Database of Korea. ITP was identified based on the diagnostic code D69.38 from the Korean Standard Classification of Diseases. Patients between one and 18 years old, who had at least one health insurance claim for ITP as a final diagnosis, from 1 January 2016, to 31 December 2017, were analyzed. Prevalent cases were defined as patients who used, at least one time, any medical services coded as D69.38. Incident cases were defined as patients who did not use D69.38 coded medical services during the prior year and were newly registered in 2017. The prevalence and incidence of ITP were 24.53 and 13.39 per 100,000 persons. The peak rates were observed in 1-year-old patients. The gender-specific prevalence of ITP was significantly higher in one-year-old males than females. According to the change-point analysis, we found that the prevalence and incidence diminished rapidly at the ages of four and three, respectively. This Korean population-based epidemiological study of ITP provided meaningful insights into the current epidemiology of ITP and demonstrated the implications of interpreting epidemiologic studies to reflect age categorizing and health care system characteristics.
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http://dx.doi.org/10.1177/0961203321995247DOI Listing
April 2021

Treatment Outcomes of Pediatric Acute Myeloid Leukemia in the Yeungnam Region: A Multicenter Retrospective Study of the Study Alliance of Yeungnam Pediatric Hematology-Oncology (SAYPH).

Children (Basel) 2021 Feb 5;8(2). Epub 2021 Feb 5.

Department of Pediatrics, Pusan National University Children's Hospital, Pusan National University, School of Medicine, Yangsan 50612, Korea.

Acute myeloid leukemia (AML) is the second most common pediatric leukemia, with a survival rate of 70%. In this retrospective study, we evaluated the treatment outcomes of pediatric AML among 144 patients diagnosed between 2000 and 2013. After induction, 80.6% of patients achieved complete remission (CR). The 5-year overall survival (OS) and event-free survival (EFS) rates were 58.8 ± 4.2% and 49.8 ± 4.2%, respectively. Based on the response to induction therapy, the 5-year OS was 66.9 ± 5.7% in patients with CR ( < 0.001). Ninety-nine patients with CR after induction therapy were examined, and their 5-year OS and EFS were 66.4 ± 4.9% and 56.3 ± 5.1%, respectively. The 5-year OS rates according to treatment were 59.9 ± 7.4% in the chemotherapy group and 72.3 ± 6.3% in the hematopoietic stem cell transplantation (HSCT) group ( = 0.089). The EFS was 50.1 ± 7.4% in the chemotherapy group and 61.7 ± 6.9% in the HSCT group ( = 0.098). OS and EFS according to cytogenetics were insignificant. Our findings confirmed that the response to induction treatment was important for survival and HSCT had no significant survival benefits compared with those of chemotherapy. Moreover, many early induction deaths under the age of 2 years were observed.
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http://dx.doi.org/10.3390/children8020109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915354PMC
February 2021

Virucidal Effects of Dielectric Barrier Discharge Plasma on Human Norovirus Infectivity in Fresh Oysters ().

Foods 2020 Nov 25;9(12). Epub 2020 Nov 25.

Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Korea.

This study investigates the effects of dielectric barrier discharge (DBD) plasma treatment (1.1 kV, 43 kHz, N 1.5 L/min, 10~60 min) on human norovirus (HuNoV) GII.4 infectivity in fresh oysters. HuNoV viability in oysters was assessed by using propidium monoazide (PMA) as a nucleic acid intercalating dye before performing a real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Additionally, the impact of the DBD plasma treatment on pH and Hunter colors was assessed. When DBD plasma was treated for 60 min, the HuNoV genomic titer reduction without PMA pretreatment was negligible (<1 log copy number/µL), whereas when PMA treatment was used, HuNoV titer was reduced to >1 log copy number/µL in just 30 min. D and D-value of HuNoV infectivity were calculated as 36.5 and 73.0 min of the DBD plasma treatment, respectively, using the first-order kinetics model (R = 0.98). The pH and Hunter colors were not significantly different ( > 0.05) between the untreated and DBD-plasma-treated oysters. The results suggest that PMA/RT-qPCR could help distinguish HuNoV infectivity without negatively affecting oyster quality following >30 min treatment with DBD plasma. Moreover, the inactivation kinetics of nonthermal DBD plasma against HuNoV in fresh oysters might provide basic information for oyster processing and distribution.
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http://dx.doi.org/10.3390/foods9121731DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760321PMC
November 2020

Effects of radioactive iodine treatment on cardiovascular disease in thyroid cancer patients: a nationwide cohort study.

Ann Transl Med 2020 Oct;8(19):1235

Division of Endocrinology and Metabolism, Department of Internal Medicine, Korea University College of Medicine, Seoul, South Korea.

Background: Radioactive iodine (RAI) treatment is a standard treatment in differentiated thyroid cancer (TC). However, its adverse effects on cardiovascular diseases (CVDs) have not been clearly elucidated.

Methods: In this retrospective cohort study based on the Korean National Health Insurance Service-National Health Screening Cohort (2002-2015), we analyzed 4,845 patients with TC with a median follow-up of 66 months. We evaluated and compared the risk of CVD between patients treated with and without RAI therapy. The primary CVD outcome was defined as a composite of ischemic stroke (IS), ischemic heart disease (IHD), hemorrhagic stroke (HS), or heart failure (HF).

Results: Overall, 2,533 patients (52.3%) received RAI treatment with a median cumulative dosage of 103 mCi [interquartile range (IQR), 40-162 mCi]. The incidence of the primary CVD outcome in patients who did not receive RAI therapy and those who did was 17.32 [95% confidence interval (CI), 15.07-19.90] and 13.96 (95% CI, 12.17-16.01) per 1,000 person-years, respectively, indicating an adjusted hazard ratio (HR) of 0.87 (95% CI, 0.71-1.07) after multivariate adjustments for variable confounding factors. The risks of IS (HR, 0.83; 95% CI, 0.51-1.34), IHD (HR, 0.90; 95% CI, 0.71-1.13), HS (HR 1.01; 95% CI, 0.49-2.09), and HF (HR 0.89; 95% CI, 0.49-1.63) were comparable between the patients who received RAI therapy and those who did not. There was no cumulative dose-dependent risk for CVD in TC patients who received RAI treatment.

Conclusions: RAI treatment is a prevalent and crucial treatment for TC, and has been used in more than half of TC patients in Korea from 2004 to 2015. This study found no significant between-group difference for the CVD risk in patients with TC who received RAI treatment and those who did not, giving further evidence to allay concerns related to the adverse effects of RAI.
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http://dx.doi.org/10.21037/atm-20-5222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607121PMC
October 2020

New Bioink Derived from Neonatal Chicken Bone Marrow Cells and Its 3D-Bioprinted Niche for Osteogenic Stimulators.

ACS Appl Mater Interfaces 2020 Nov 21;12(44):49386-49397. Epub 2020 Oct 21.

College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 16419, Korea.

This study examined whether neonatal chicken bone marrow cells (cBMCs) could support the osteogenesis of human stromal cells in a three-dimensional (3D) extracellular bioprinting niche. The majority (>95%) of 4-day-old cBMCs subcultured 5 times were positive for osteochondrogenesis-related genes (, , , aggrecan, , osterix, , osteocalcin, , and osteopontin) and their related proteins (Sox9, collagen type I, and collagen type II). LC-MS/MS analysis demonstrated that cBMC-conditioned medium (c-medium) contained proteins related to bone regeneration, such as periostin and members of the TGF-β family. Next, a significant increase in osteogenesis was detected in three human adipose tissue-derived stromal cell (hASC) lines, after exposure to c-medium concentrates in 2D culture ( < 0.05). To evaluate biological function in a 3D environment, we employed the cBMC-derived bioactive components as a cell-supporting biomaterial in collagen bioink, which was printed to construct a 3D hASC-laden scaffold for observing osteogenesis. Complete osteogenesis was detected in vitro. Moreover, after transplantation of the hASC-laden structure into rats, prominent bone formation was observed compared with that in control rats receiving scaffold-free hASC transplantation. These results demonstrated that substance(s) secreted by chick bone marrow cells clearly activated the osteogenesis of hASCs in 2D- or 3D-niches.
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http://dx.doi.org/10.1021/acsami.0c13905DOI Listing
November 2020

Prediction of Risk Factors after Spine Surgery in Patients Aged >75 Years Using the Modified Frailty Index.

J Korean Neurosurg Soc 2020 Nov 9;63(6):827-833. Epub 2020 Sep 9.

Department of Anesthesiology, Pain and Critical Care Medicine, Yeouido St. Mary's Hospital, College of Medicine, Catholic University of Korea, Seoul, Korea.

Objective: Spine surgery is associated with higher morbidity and mortality rates in elderly patients. The modified Frailty Index (mFI) is an evaluation tool to determine the frailty of an individual and how preoperative status may impact postoperative survival and outcomes. This study aimed to determine the usefulness of mFI in predicting postoperative complications in patients aged ≥75 years undergoing surgery with instrumentation.

Methods: We retrospectively reviewed the perioperative course of 137 patients who underwent thoracolumbar-instrumentation spine surgery between 2011 and 2016. The preoperative risk factors were the 11 variables of the mFI, as well as body mass index (kg/cm2), preoperative hemoglobin, platelet, albumin, creatinine, anesthesia time, operation time, estimated blood loss, and transfusion amount. The 60-day occurrences of complication rates were used for outcome assessment.

Results: Major complications after spinal instrumentation surgery occurred in 34 of 138 patients (24.6%). The mean mFI score was 0.18±0.12. When we divided patients into a pre-frail group (mFI, 0.09-0.18; n=94) and a frail group (mFI ≥0.27; n=44), only the rate of sepsis was statistically higher in the frail group than in the pre-frail group. There were significantly more major complications in patients with low albumin levels or in patients with infection or who had experienced trauma. The mFI was a more useful predictor of postoperative complications than the American Society of Anesthesiologists physical status score.

Conclusion: The mFI can successfully predict postoperative morbidity and mortality in patients aged ≥75 years undergoing spine surgery. The mFI improves perioperative risk stratification that provides important information to assist in the preoperative counselling of patients and their families.
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http://dx.doi.org/10.3340/jkns.2020.0019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671779PMC
November 2020

Circulating miRNAs Associated with Dysregulated Vascular and Trophoblast Function as Target-Based Diagnostic Biomarkers for Preeclampsia.

Cells 2020 08 31;9(9). Epub 2020 Aug 31.

Departments of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea.

Preeclampsia (PE) is a pregnancy-specific disorder associated with hypertension and proteinuria. Since there is no proven method to treat PE, early prediction and accurate diagnosis are essential for appropriate management of the disease. Thus, reliable biomarkers for diagnosing PE need to be identified and evaluated. We analyzed serum-soluble factors and miRNAs in 92 patients with PE and an equal number of healthy controls to identify new useful biomarkers for PE. Serum miR-31-5p, miR-155-5p, and miR-214-3p levels were significantly elevated in these patients and highly correlated with clinical symptoms of hypertension and proteinuria, whereas the miR-1290-3p level was decreased. The increased miRNAs were upregulated in an NF-κB-dependent manner and suppressed endothelial nitric oxide synthase (eNOS) and placental growth factor (PlGF) expression. The level of each miRNA had greater than 90% diagnostic accuracy, which was further increased by analyzing its ratio relative to that of miR-1290-3p. Taken together, the ratios of miR-31-5p, miR-155-5p, or miR-214-3p to miR-1290-3p may serve as reliable diagnostic or prognostic tools for PE, thereby providing evidence that these miRNAs are promising mechanism-based targets for designing therapeutic and preventive strategies for the clinical management of PE.
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http://dx.doi.org/10.3390/cells9092003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565195PMC
August 2020

Epidemiological Study of Hereditary Hemolytic Anemia in the Korean Pediatric Population during 1997-2016: a Nationwide Retrospective Cohort Study.

J Korean Med Sci 2020 Aug 24;35(33):e279. Epub 2020 Aug 24.

Department of Pediatrics, Korea University Ansan Hospital, Korea University College of Medicine, Ansan, Korea.

Background: Hereditary hemolytic anemia (HHA) is a rare disease characterized by premature red blood cell (RBC) destruction due to intrinsic RBC defects. The RBC Disorder Working Party of the Korean Society of Hematology established and updated the standard operating procedure for making an accurate diagnosis of HHA since 2007. The aim of this study was to investigate a nationwide epidemiology of Korean HHA.

Methods: We collected the data of a newly diagnosed pediatric HHA cohort (2007-2016) and compared this cohort's characteristics with those of a previously surveyed pediatric HHA cohort (1997-2006) in Korea. Each participant's information was retrospectively collected by a questionnaire survey.

Results: A total of 369 children with HHA from 38 hospitals distributed in 16 of 17 districts of Korea were investigated. RBC membranopathies, hemoglobinopathies, RBC enzymopathies, and unknown etiologies accounted for 263 (71.3%), 59 (16.0%), 23 (6.2%), and 24 (6.5%) of the cases, respectively. Compared to the cohort from the previous decade, the proportions of hemoglobinopathies and RBC enzymopathies significantly increased ( < 0.001 and = 0.008, respectively). Twenty-three of the 59 hemoglobinopathy patients had immigrant mothers, mostly from South-East Asia.

Conclusion: In Korea, thalassemia traits have increased over the past 10 years, reflecting both increased awareness of this disease and increased international marriages. The enhanced recognition of RBC enzymopathies is due to advances in diagnostic technique; however, 6.5% of HHA patients still do not have a clear diagnosis. It is necessary to improve accessibility of diagnosing HHA.
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http://dx.doi.org/10.3346/jkms.2020.35.e279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445306PMC
August 2020

NF-κB-dependent miR-31/155 biogenesis is essential for TNF-α-induced impairment of endothelial progenitor cell function.

Exp Mol Med 2020 08 7;52(8):1298-1309. Epub 2020 Aug 7.

Department of Molecular and Cellular Biochemistry, Kangwon National University School of Medicine, Chuncheon, Gangwon-do, 24341, South Korea.

Endothelial progenitor cell (EPC) dysfunction impairs vascular function and remodeling in inflammation-associated diseases, including preeclampsia. However, the underlying mechanism of this inflammation-induced dysfunction remains unclear. In the present study, we found increases in TNF-α and miR-31/155 levels and reduced numbers of circulating EPCs in patients with preeclampsia. Patient-derived mononuclear cells (MNCs) cultured in autologous serum had decreased endothelial nitric oxide synthase (eNOS) expression, nitric oxide production, and differentiation into EPCs with angiogenic potential, and these effects were inhibited by a TNF-α-neutralizing antibody and miR-31/155 inhibitors. Moreover, TNF-α treatment of normal MNCs increased miR-31/155 biogenesis, decreased eNOS expression, reduced EPC differentiation, and impaired angiogenic potential. The TNF-α-induced impairment of EPC differentiation and function was rescued by NF-κB p65 knockdown or miR-31/155 inhibitors. In addition, treatment of MNCs with synthetic miR-31/155 or an eNOS inhibitor mimicked the inhibitory effects of TNF-α on eNOS expression and EPC functions. Moreover, transplantation of EPCs that had been differentiated from TNF-α-treated MNCs decreased neovascularization and blood perfusion in ischemic mouse hindlimbs compared with those of normally differentiated EPCs. These findings suggest that NF-κB activation is required for TNF-α-induced impairment of EPC mobilization, differentiation, and function via miR-31/155 biogenesis and eNOS downregulation. Our data provide a new role for NF-κB-dependent miR-31/155 in EPC dysfunction under the pathogenic conditions of inflammation-associated vascular diseases, including preeclampsia.
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http://dx.doi.org/10.1038/s12276-020-0478-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080610PMC
August 2020

The Efficiency of Atmospheric Dielectric Barrier Discharge Plasma against and on Dried Laver ().

Foods 2020 Jul 28;9(8). Epub 2020 Jul 28.

Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Korea.

This study investigated the effects of atmospheric dielectric barrier discharge (DBD) plasma (1.1 kV, 43 kHz, 5-30 min, N: 1.5 L/m) on the reduction of and on dried laver. The reductions of and by 5, 10, 20, and 30 min of DBD plasma were 0.56 and 0.24, 0.61 and 0.66, 0.76 and 1.24, and 1.02 and 1.38 log CFU/g, respectively. The D-value of and was predicted as 29.80 and 20.53 min, respectively, using the Weibull model for (R = 0.95) and first-order kinetics for (R = 0.94). After DBD plasma 5-30 min treatment, there was no change in pH (6.20-6.21) and this value was higher than the untreated dried laver (6.08). All sensory scores in DBD plasma-treated laver were determined as >6 points. The 30 min of DBD plasma is regarded as a novel intervention for the control of potential hazardous bacteria in dried laver.
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http://dx.doi.org/10.3390/foods9081013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466277PMC
July 2020

Diplopia developed by cervical traction after cervical spine surgery.

Yeungnam Univ J Med 2021 Apr 29;38(2):152-156. Epub 2020 Jul 29.

Department of Neurosurgery, School of Medicine, Kyungpook National University, Kyungpook National University Chilgok Hospital, Daegu, Korea.

Diplopia is a rare complication of spine surgery. The abducens nerve is one of the cranial nerves most commonly related to diplopia caused by traction injury. We report a case of a 71-year-old woman who presented with diplopia developing from abducens nerve palsy after C1-C2 fixation and fusion due to atlantoaxial subluxation with cord compression. As soon as we discovered the symptoms, we suspected excessive traction by the instrument and subsequently performed reoperation. Subsequently, the patient's symptoms improved. In other reported cases we reviewed, most were transient. However, we thought that our rapid response also helped the patient's fast recovery in this case. The mechanisms by which postoperative diplopia develops vary and, thus, remain unclear. We should pay attention to the fact that the condition is sometimes an indicator of an underlying, life-threatening condition. Therefore, all patients with postoperative diplopia should undergo thorough ophthalmological and neurological evaluations as well as careful observation by a multidisciplinary team.
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http://dx.doi.org/10.12701/yujm.2020.00241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8016621PMC
April 2021

Development and Usability Evaluation of a Facebook-Based Intervention Program for Childhood Cancer Patients: Mixed Methods Study.

J Med Internet Res 2020 07 28;22(7):e18779. Epub 2020 Jul 28.

School of Nursing (retired), University of Maryland Baltimore, Baltimore, MD, United States.

Background: Childhood cancers previously considered to be incurable now have 5-year survival rates up to 84%. Nevertheless, these patients remain at risk of morbidity and mortality from therapy-related complications. Thus, patient education and self-management strategies for promoting a healthy lifestyle are of tantamount importance for improving short- and long-term health outcomes. A Facebook-based "Healthy Teens for Soaam" (a Korean term for childhood cancers) program was developed to help improve knowledge and self-management practices of teens with cancer related to their disease and treatment.

Objective: The two-fold purpose of this usability study was (1) to describe the process of developing an 8-week Facebook-based intervention program for teens with cancer, and (2) to evaluate its usability to refine the program.

Methods: Multiple phases and methods were employed to develop and evaluate the usability of the program. Study phases included: (1) needs assessment through focus group interviews and qualitative content analysis, (2) development of module content, (3) expert review and feedback on module content, (4) Facebook-based program development, (5) usability evaluation by heuristic evaluation, (6) usability evaluation by targeted end-user testing, and (7) modification and final version of the program. Usability of the final version was confirmed through feedback loops of these phases.

Results: Based on 6 focus group discussion sessions, it was determined that teens with cancer were interested in seeing stories of successful childhood cancer cases and self-management after discharge, and preferred multimedia content over text. Therefore, each Facebook module was redesigned to include multimedia materials such as relevant video clips tailored for teens. Usability assessed by heuristic evaluation and user testing revealed several critical usability issues, which were then revised. Potential end users tested the final program and perceived it to be usable and useful for teens with cancer.

Conclusions: To our knowledge, "Healthy Teens for Soaam" is the first Facebook-based intervention program for teens with cancer. We actively worked with current childhood cancer patients and survivors to develop and improve this program, achieved good usability, and met the expressed needs and preferences of target end users. This 8-week Facebook-based educational program for teens with cancer, developed as the first step of an upcoming intervention study, will be useful for improving knowledge and self-management strategies of teens.
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http://dx.doi.org/10.2196/18779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7420636PMC
July 2020

Responses of lake macrophyte species and functional traits to climate and land use changes.

Sci Total Environ 2020 Sep 23;736:139628. Epub 2020 May 23.

Department of Environmental Science, Toho University, Funabashi 274-8510, Japan; Center for Climate Change Adaptation, National Institute for Environmental Studies, Tsukuba 305-8506, Japan.

Aquatic plants are essential components in the regulation of microhabitat complexity and physico-chemical parameters in lake ecosystems. Increased eutrophication, land use change, modification of hydrological regimes, and expansion of invasive species are expected to impact aquatic plant community composition; however, historical pathways and response patterns are not well understood at the national scale. We analyzed temporal changes in aquatic plant communities in Japan from the early 1900s to the 2000s using field survey records from 248 lakes. Relationships of species associations with climate, land use, and lake characteristics were described using a joint species distribution model. The mean variation attributable to lake characteristics was 25.4%, followed by climate (14.0%), and land use (10.5%). Among the 13 functional traits used in our analysis, sexual and pollination traits showed marked responses to precipitation and land use. Hypohydrophily increased with precipitation, whereas monoecious aquatic plants increased in lakes surrounded by urbanized area. The relative ratio of floating to submerged plants has increased over time. Our results provide insight into long-term changes in aquatic plant communities and identify functional traits sensitive to environmental change.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139628DOI Listing
September 2020

The influence of surrounding land cover on wetland habitat conditions: a case study of inland wetlands in South Korea.

PeerJ 2020 18;8:e9101. Epub 2020 May 18.

Department of Integrated Biological Sciences, Pusan National University, Busan, South Korea.

Wetland ecosystems have been globally degraded and lost due to rapid urbanization and climate change. An assessment of national scale inventory, including wetland types and conditions, is urgently required to understand the big picture of endangered wetlands, such as where they are and how they look like. We analyzed the spatial patterns of each inland wetland type (brackish wetland was included) in South Korea and the relative importance of land cover categories on wetland conditions. The wetlands were grouped into four dominant types (riverine, lake, mountain, and human-made) according to their topography. Riverine wetlands constituted the largest area (71.3%). The relative ratio of wetlands in a well-conserved condition (i.e., "A" rank) was highest in riverine wetlands (23.8%), followed by mountain wetlands (22.1%). The higher proportion of grasslands was related to a better condition ranking, but the increasing bareland area had a negative impact on wetland conditions. We also found that wetlands located near wetland protected areas tend to be in a better condition compared to remote sites. Our results further support the importance of the condition of surrounding areas for wetland conservation.
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http://dx.doi.org/10.7717/peerj.9101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241414PMC
May 2020

Combined treatment of surgery and sclerotherapy for sinus pericranii.

Arch Craniofac Surg 2020 Apr 20;21(2):109-113. Epub 2020 Apr 20.

Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, Daegu, Korea.

Sinus pericranii is a rare vascular anomaly characterized by abnormal venous communication between the inner and outer regions of the cranial cavity. Here, we report a case of sinus pericranii and venous malformations in the right periorbital region of a 2-year-old girl. Radiologic findings showed venous malformations in the right parietal region communicating with the superior sagittal sinus in the intracranial region. There were notable improvements following surgical resection for the abnormal venous lesions and several sclerotherapies. Presence of a bluish and pulsating mass on the scalp, which showed bruit on auscultation, may indicate sinus pericranii, which should be included in the differential diagnosis.
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http://dx.doi.org/10.7181/acfs.2019.00521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206457PMC
April 2020

Incidence and Prevalence of Juvenile Systemic Lupus Erythematosus in Korea: Data From the 2017 National Health Claims Database.

J Rheumatol 2021 02 1;48(2):258-261. Epub 2020 May 1.

J.Y. Kim, MD, PhD Department of Pediatrics, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.

Objective: The purpose of the present study was to investigate the prevalence and incidence of juvenile systemic lupus erythematosus (JSLE) in Korea.

Methods: The data were collected from the National Health Insurance Claims Database of Korea. JSLE was identified using the diagnostic code M32 from the Korean Standard Classification of Diseases. Patients between 5 and 18 years old, who had at least 1 claim for JSLE from January 1, 2016, to December 31, 2017, as final diagnosis, were analyzed in the study. For prevalent cases, patients who used, at least 1 time, any type of medical services with a diagnostic code of M32 were selected. For incident cases, patients who did not use medical services with the M32 code 1 year prior and who were newly registered in 2017 were defined. Change-point analysis was used to find the age at which changes in prevalence and incidence occurred.

Results: The prevalence of JSLE was 6.92 per 100,000 persons and the incidence of JSLE was 2.76 per 100,000 person-years in patients between 5 and 18 years old. The prevalence and incidence of JSLE were higher in females than in males. According to the change-point analysis, we found that the incidence and prevalence of female patients increased rapidly at the ages of 14 and 15 years, respectively.

Conclusion: This Korean population-based epidemiological study of JSLE showed similar epidemiologic profiles to Asian population in other studies. The distribution of age, ethnicity, and pubertal status are important factors that influence population estimates of JSLE incidence and prevalence.
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http://dx.doi.org/10.3899/jrheum.191186DOI Listing
February 2021

Comparisons of food security, dietary behaviors and nutrient intakes between adult North Korean Refugees in South Korea and South Koreans.

Nutr Res Pract 2020 Apr 3;14(2):134-142. Epub 2020 Mar 3.

Department of Internal Medicine, College of Medicine, Korea University, 73, Inchon-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.

Background/objectives: North Korean refugees (NKRs) in South Korea are a unique population as they must adapt in a new country with similar cultural traits but different social, political, and economic systems, but little research has been conducted on diet and nutrition in this population. This study examined food security, dietary behaviors, and nutrient intakes among adult NKRs living in South Korea and compared them to those of South Koreans.

Subjects/methods: The subjects were 139 adult NKRs (25 men, 114 women) living in the Seoul metropolitan area, and 417 age- and sex-matched South Korean controls (SKCs; 75 men, 342 women) selected from the Korea National Health and Nutrition Examination Survey (KNHANES). Food security and dietary behaviors (meal skipping, eating-out, meals with family, nutrition education and counseling, and nutrition label knowledge and utilization) were obtained using self-administered questionnaires. Nutrient intakes were assessed by 24-hr recall. The statistical analysis was performed using IBM SPSS ver. 23.0.

Results: In South Korea, food security had improved over the previous 12 months, but remained significantly poorer for NKR women than SKC women. Meal skipping was three times more frequent than for SKCs and eating-out was rare. Average energy intake was 1,509 kcal for NKR men and 1,344 kcal for NKR women, which was lower than those of SKCs (2,412 kcal and 1,789 kcal, respectively). Significantly more NKRs (men 24.0%, women 21.9%) showed simultaneously deficient intake in energy, calcium, iron, vitamin A, and riboflavin than SKCs (men 2.7% ( = 0.003), women 7.0% ( < 0.001)). NKR women had a significantly higher index of nutrient quality (INQ) for some nutrients than SK women.

Conclusions: This study reports significant differences in food security, dietary behaviors, and nutrient intakes between NKRs and SKCs. Generally, NKRs reported lower intakes despite improved food security, but relatively good INQs across nutrients. Further research is needed to understand processes of food choice and consumption among NKRs to provide appropriate support aimed at improving diets.
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http://dx.doi.org/10.4162/nrp.2020.14.2.134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7075740PMC
April 2020

Lipofundin MCT/LCT Inhibits Levcromakalim-Induced Vasodilation by Inhibiting Endothelial Nitric Oxide Release.

Int J Mol Sci 2020 Mar 4;21(5). Epub 2020 Mar 4.

Department of Anesthesiology and Pain Medicine, Gyeongsang National University College of Medicine, Gyeongsang National University Hospital, 15 Jinju-daero 816 Beon-gil, Jinju-si 52727, Korea.

The goal of this study was to examine the effect of lipid emulsion on the vasodilation induced by ATP-sensitive potassium (K) channels in isolated rat aortae and the underlying mechanism. The effects of Intralipid, containing 100% long-chain fatty acids, and Lipofundin MCT/LCT, containing 50% long-chain fatty acids plus 50% medium-chain fatty acids, on the vasodilation induced by levcromakalim in endothelium-intact aorta with or without N-nitro-L-arginine methyl ester (L-NAME) and in endothelium-denuded aorta were examined. The effects of L-arginine, L-NAME, glibenclamide, and Lipofundin MCT/LCT, alone or combined, on the levcromakalim-induced vasodilation were examined. Lipofundin MCT/LCT inhibited the levcromakalim-induced vasodilation of isolated endothelium-intact aortae, whereas Intralipid did not. In addition, Lipofundin MCT/LCT had no effect on the levcromakalim-induced vasodilation of endothelium-denuded rat aortae and endothelium-intact aortae with L-NAME. L-arginine and Lipofundin MCT/LCT produced more levcromakalim-induced vasodilation than Lipofundin MCT/LCT alone. Glibenclamide inhibited levcromakalim-induced vasodilation. Levcromakalim did not significantly alter endothelial nitric oxide synthase phosphorylation, whereas Lipofundin MCT/LCT decreased cyclic guanosine monophosphate. Lipofundin MCT/LCT did not significantly alter levcromakalim-induced membrane hyperpolarization. Taken together, these results suggest that Lipofundin MCT/LCT inhibits the vasodilation induced by levcromakalim by inhibiting basally released endothelial nitric oxide, which seems to occur through medium-chain fatty acids.
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http://dx.doi.org/10.3390/ijms21051763DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084418PMC
March 2020

Treatment Outcome in Pediatric Acute Lymphoblastic Leukemia With Hyperleukocytosis in the Yeungnam Region of Korea: A Multicenter Retrospective Study.

J Pediatr Hematol Oncol 2020 05;42(4):275-280

Department of Pediatrics, Pusan National University School of Medicine, Yangsan.

Acute lymphoblastic leukemia (ALL) with hyperleukocytosis at diagnosis is associated with early morbidity and mortality due to complications of leukostasis. Of 535 pediatric ALL patients (January 2004 to December 2016 from the Yeungnam region of Korea), 72 (13.5%) patients with an initial white blood cell (WBC) count of ≥100×10/L were included in this study, of whom 38 patients had extreme hyperleukocytosis (WBC>200×10/L) at diagnosis. Fourteen patients (19.4%) had ≥1 early respiratory and neurologic complications during induction therapy. Relapse occurred in 8 patients (24.2%) with extreme hyperleukocytosis and in 1 patient (3.0%) with an initial WBC count of 100 to 200×10/L (P=0.012). Estimated 10-year event-free survival rate (EFS) and overall survival rate were 78.3%±8.4% and 82.6%±7.7%, respectively. The 10-year EFS was significantly lower in patients with an initial WBC count of >200×10/L than in those with an initial WBC count of 100 to 200×10/L (65.7%±13.4% vs. 91.2%±7.9%; P=0.011). The 10-year EFS and overall survival rate did not differ significantly between patients with extreme hyperleukocytosis who received hematopoietic stem cell transplantation and those who received chemotherapy. In conclusion, pediatric ALL with hyperleukocytosis can lead to early complications and mortality. Patients with initial extreme hyperleukocytosis showed significantly poorer prognosis than those with WBC counts of 100 to 200×10/L.
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http://dx.doi.org/10.1097/MPH.0000000000001771DOI Listing
May 2020

Synergistic Effects of Mild Heating and Dielectric Barrier Discharge Plasma on the Reduction of in Red Pepper Powder.

Foods 2020 Feb 11;9(2). Epub 2020 Feb 11.

Department of Seafood and Aquaculture Science/Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Korea.

The synergistic efficacy of a combined treatment of mild heat (MH) and dielectric barrier discharge (DBD) plasma in Bacillus cereus-contaminated red pepper powder was tested. A cocktail of three strains of B. cereus (NCCP 10623, NCCP 14579, ATCC 11778) was inoculated onto red pepper powder and then treated with MH (60 °C for 5-20 min) and DBD plasma (5-20 min). Treatment with MH and DBD plasma alone for 5-20 min resulted in reductions of 0.23-1.43 and 0.12-0.96 log CFU/g, respectively. Combined treatment with MH and DBD plasma was the most effective at reducing B. cereus counts on red pepper powder, and resulted in log-reductions of ≥ 6.0 log CFU/g. The largest synergistic values (4.24-4.42 log) against B. cereus in red pepper powder were obtained by the combination of 20 min MH and 5-15 min DBD plasma. The values of Hunter color ''L'', ''a'', and ''b'', were not significantly different from those of the untreated sample and that with the combination of MH (60 °C for 5-20 min) and DBD plasma (5-20 min). Also, no significant (p > 0.05) differences in pH values between samples were observed. Therefore, these results suggest that the combination of MH treatment and DBD plasma can be potentially utilized in the food industry to effectively inactivate B. cereus without incurring quality deterioration of red pepper powder.
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http://dx.doi.org/10.3390/foods9020171DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073615PMC
February 2020

Korean parents' perceptions of the challenges and needs on school re-entry during or after childhood and adolescent cancer: a multi-institutional survey by Korean Society of Pediatric Hematology and Oncology.

Clin Exp Pediatr 2020 Apr 14;63(4):141-145. Epub 2019 Nov 14.

Department of Pediatrics, Dankook University College of Medicine, Cheonan, Korea.

Background: For children and adolescents with cancer, going back to school is a key milestone in returning to "normal life."

Purpose: To identify the support vital for a successful transition, we evaluated the parents' needs and the challenges they face when their children return to school.

Methods: This multi-institutional study was conducted by the Korean Society of Pediatric Hematology and Oncology. The written survey comprised 24 questions and was completed by 210 parents without an interviewer.

Results: Most parents (165 of 206) reported that their children experienced difficulties with physical status (n=60), peer relationships (n=30), academic performance (n=27), emotional/behavioral issues (n=11), and relationships with teachers (n=4) on reentering school. Parents wanted to be kept informed about and remain involved in their children's school lives and reported good parent-teacher communication (88 of 209, 42.1%). Parents reported that 83.1% and 44.9% of teachers and peers, respectively, displayed an adequate understanding of their children's condition. Most parents (197 of 208) answered that a special program is necessary to facilitate return to school after cancer therapy that offers emotional support (n=85), facilitates social adaptation (n=61), and provides tutoring to accelerate catch up (n=56), and continued health care by hospital outreach and school personnel (n=50).

Conclusion: In addition to scholastic aptitude-oriented programs, emotional and psychosocial support is necessary for a successful return to school. Pediatric oncologists should actively improve oncology practices to better integrate individualized school plans and educate peers and teachers to improve health literacy to aid them in understanding the needs of children with cancer.
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http://dx.doi.org/10.3345/kjp.2019.00696DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7170787PMC
April 2020