Publications by authors named "Ji Yang"

717 Publications

Monoterpene-flavonoid conjugates from Sarcandra glabra and their autophagy modulating activities.

Bioorg Chem 2021 Mar 18;112:104830. Epub 2021 Mar 18.

State Key Laboratory of Quality Research in Chinese Medicine, Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Disease, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau. Electronic address:

Fourteen new monoterpene-flavonoid conjugates including four monoterpene-conjugated chalcones (glabratins A-D, 1-4), seven monoterpene-conjugated dihydrochalcones (glabratins E-K, 5-11), and three monoterpene-conjugated flavanones (glabratins L-N, 12-14), together with four known analogues (15-18) were isolated from the aerial parts of Sarcandra glabra. The structures and the absolute configurations of these compounds were elucidated by the spectroscopic data, single-crystal X-ray diffraction, and electronic circular dichroism (ECD) calculations. Compounds 1, 4-6, 9-14, and 18 showed obvious cell autophagy-inducing activities at 25 μM in HEK293 cells. Furthermore, the bioassay results also showed that 18 induced cell autophagy in a dose dependent manner. Our findings revealed a rare class of monoterpene-flavonoid conjugates in nature and firstly reported their autophagy-inducing activities.
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http://dx.doi.org/10.1016/j.bioorg.2021.104830DOI Listing
March 2021

Lutonarin from Barley Seedlings Inhibits the Lipopolysacchride-Stimulated Inflammatory Response of RAW 264.7 Macrophages by Suppressing Nuclear Factor-κB Signaling.

Molecules 2021 Mar 12;26(6). Epub 2021 Mar 12.

Division of Crop Foundation, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Wanju 55365, Korea.

Extracts from barley seedlings (BS) have known antioxidant and anti-inflammatory activities. The flavonoid lutonarin (LN) is a component of BS extract and has several known bioactivities. Here, we evaluated LN anti-inflammatory efficacy against lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Lutonarin was isolated from BS by methanol extraction and characterized by ultra-performance liquid chromatography and quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). Lutonarin did not reduce the viability or enhance the apoptosis rate of RAW 264.7 macrophages at concentrations up to 150 µM. Concentrations within 20-60 µM dose-dependently suppressed the LPS-induced expression, phosphorylation, and nuclear translocation of the inflammatory transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Furthermore, LN suppressed the LPS-induced upregulation of proinflammatory cytokines interleukin (IL)-6 and tumor necrosis factor (TNF)-α and of the inflammatory enzyme cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Lutonarin may be a safe and effective therapeutic agent for alleviation of pathological inflammation.
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http://dx.doi.org/10.3390/molecules26061571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999162PMC
March 2021

DRER: Deep Learning-Based Driver's Real Emotion Recognizer.

Sensors (Basel) 2021 Mar 19;21(6). Epub 2021 Mar 19.

Department of Automobile and IT Convergence, Kookmin University, 77, Jeongneung-ro, Seongbuk-gu, Seoul 02707, Korea.

In intelligent vehicles, it is essential to monitor the driver's condition; however, recognizing the driver's emotional state is one of the most challenging and important tasks. Most previous studies focused on facial expression recognition to monitor the driver's emotional state. However, while driving, many factors are preventing the drivers from revealing the emotions on their faces. To address this problem, we propose a deep learning-based driver's real emotion recognizer (DRER), which is a deep learning-based algorithm to recognize the drivers' real emotions that cannot be completely identified based on their facial expressions. The proposed algorithm comprises of two models: (i) facial expression recognition model, which refers to the state-of-the-art convolutional neural network structure; and (ii) sensor fusion emotion recognition model, which fuses the recognized state of facial expressions with electrodermal activity, a bio-physiological signal representing electrical characteristics of the skin, in recognizing even the driver's real emotional state. Hence, we categorized the driver's emotion and conducted human-in-the-loop experiments to acquire the data. Experimental results show that the proposed fusing approach achieves 114% increase in accuracy compared to using only the facial expressions and 146% increase in accuracy compare to using only the electrodermal activity. In conclusion, our proposed method achieves 86.8% recognition accuracy in recognizing the driver's induced emotion while driving situation.
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http://dx.doi.org/10.3390/s21062166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003797PMC
March 2021

Evodiamine Inhibits Growth and -Induced Inflammation.

Int J Mol Sci 2021 Mar 25;22(7). Epub 2021 Mar 25.

Department of Clinical Laboratory Science, Semyung University, Jecheon 27136, Korea.

() classified as a class I carcinogen by the World Health Organization (WHO) plays an important role in the progression of chronic gastritis and the development of gastric cancer. A major bioactive component of , evodiamine, has been known for its anti-bacterial effect and anti-cancer effects. However, the inhibitory effect of evodiamine against is not yet known and the inhibitory mechanisms of evodiamine against gastric cancer cells are yet to be elucidated concretely. In this study, therefore, anti-bacterial effect of evodiamine on growth and its inhibitory mechanisms as well as anti-inflammatory effects and its mechanisms of evodiamine on -induced inflammation were investigated in vitr. Results of this study showed the growth of the reference strains and clinical isolates were inhibited by evodiamine. It was considered one of the inhibitory mechanisms that evodiamine downregulated both gene expressions of replication and transcription machineries of . Treatment of evodiamine also induced downregulation of urease and diminished translocation of cytotoxin-associated antigen A (CagA) and vacuolating cytotoxin A (VacA) proteins into gastric adenocarcinoma (AGS) cells. This may be resulted from the reduction of CagA and VacA expressions as well as the type IV secretion system (T4SS) components and secretion system subunit protein A (SecA) protein which are involved in translocation of CagA and VacA into host cells, respectively. In particular, evodiamine inhibited the activation of signaling proteins such as the nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) and the mitogen-activated protein kinase (MAPK) pathway induced by infection. It consequently might contribute to reduction of interleukin (IL)-8 production in AGS cells. Collectively, these results suggest anti-bacterial and anti-inflammatory effects of evodiamine against .
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http://dx.doi.org/10.3390/ijms22073385DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8036659PMC
March 2021

Clay-hydrochar composites mitigated CH and NO emissions from paddy soil: A whole rice growth period investigation.

Sci Total Environ 2021 Mar 18;780:146532. Epub 2021 Mar 18.

Key Laboratory of Agro-Environment in Downstream of Yangtze Plain and Key Laboratory for Crop and Animal Integrated Farming of Ministry of Agriculture and Rural Affairs, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

With the favorable microporous structure and excellent adsorption capacity, clay-hydrochar composites (CHCs) serve as promising materials to mitigate greenhouse gas emissions (GHG) from the paddy fields. Three clays were co-pyrolyzed with hydrochar derived from poplar sawdust to obtain CHCs, which were applied to the paddy fields to investigate the effects on methane (CH) and nitrous oxide (NO) emissions. Three CHCs were labeled as bentonite-hydrochar composite (BTHC), montmorillonite-hydrochar composite (MTHC), and kaolinite-hydrochar composite (KTHC), respectively. The effects of these three CHCs on GHG emissions were determined by monitoring the dynamic CH and NO emissions in the paddy soil column ecosystem during the rice-growing season. The results showed that compared with the control group, three CHCs significantly mitigated CH and NO emissions by 21.4%-47.5% and 5.2%-36.8%, respectively. Furthermore, the fluorescent components result displayed CHCs increased humic-like content by 29.62%-59.72%. A structural equation model was used to assess the hypothesis mitigation mechanism, which exemplified that GHG emissions negatively correlated with pmoA and nosZ genes, possibly resulting in the CH and NO mitigation. Among the three CHCs, the KTHC amendment mitigated the CH and NO emissions by 47.5% and 36.8%, respectively, which was superior to BTHC and MTHC. Hence, it was recommended for application to the field. Overall, this study demonstrates the mitigating effects of CHCs on GHG emissions for the first time, and the reduced CH and NO emissions could contribute to increased soil C and N retention for better agricultural nutrients management.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146532DOI Listing
March 2021

Reversible -Acetyl Migration within the Sialic Acid Side Chain and Its Influence on Protein Recognition.

ACS Chem Biol 2021 Mar 26. Epub 2021 Mar 26.

Department of Chemistry, University of California, Davis, California 95616, United States.

-Acetylation is a common naturally occurring modification of carbohydrates and is especially widespread in sialic acids, a family of nine-carbon acidic monosaccharides. -Acetyl migration within the exocyclic glycerol-like side chain of mono--acetylated sialic acid reported previously was from the C7- to C9-hydroxyl group with or without an 8--acetyl intermediate, which resulted in an equilibrium that favors the formation of the 9--acetyl sialic acid. Herein, we provide direct experimental evidence demonstrating that -acetyl migration is bidirectional, and the rate of equilibration is influenced predominantly by the pH of the sample. While the -acetyl group on sialic acids and sialoglycans is stable under mildly acidic conditions (pH < 5, the rate of -acetyl migration is extremely low), reversible -acetyl migration is observed readily at neutral pH and becomes more significant when the pH increases to slightly basic. Sialoglycan microarray studies showed that esterase-inactivated porcine torovirus hemagglutinin-esterase bound strongly to sialoglycans containing a more stable 9--acetylated sialic acid analog, but these compounds were less resistant to periodate oxidation treatment compared to their 9--acetyl counterparts. Together with prior studies, the results support the possible influence of sialic acid -acetylation and -acetyl migration to host-microbe interactions and potential application of the more stable synthetic -acetyl mimics.
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http://dx.doi.org/10.1021/acschembio.0c00998DOI Listing
March 2021

The oncogene Mct-1 promotes progression of hepatocellular carcinoma via enhancement of Yap-mediated cell proliferation.

Cell Death Discov 2021 Mar 22;7(1):57. Epub 2021 Mar 22.

Hepatobiliary Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.

Malignant T-cell-amplified sequence 1 (Mct-1) has been reported as an oncogene in multiple malignant diseases. However, the function of Mct-1 in hepatocellular carcinoma (HCC) and the molecular mechanisms underlying tumor progression have not been explored. In this study, Mct-1 expression levels in HCC tissues and cells were detected by quantitative real-time PCR and western blotting. Mct-1 shRNAs and overexpression vector were transfected into HCC cells to downregulate or upregulate Mct-1 expression. In vitro and in vivo assays were performed to investigate the function of Mct-1 in cell proliferation and apoptosis. RNA sequencing analysis (RNA-seq) was performed to explore differences in gene expression when silenced Mct-1 expression. Mct-1 was upregulated in HCC specimens and cell lines, and higher expression of Mct-1 was predictive of poor survival. Overexpression of Mct-1 was shown to promote cell proliferation and repress cell apoptosis both in vitro and in vivo. The results of RNA-seq indicated that knockdown of Mct-1 suppressed Yap expression, while the results of the luciferase assay also revealed that Mct-1 increases the activity of the Yap promoter. Restoration of Yap expression in Mct-1 knockdown cells partially recovered the promotion of cell proliferation and inhibition of apoptosis. Collectively, these results indicate that Mct-1 acts as a tumor promoter gene in HCC progression by up-regulating Yap expression and, thus, could serve a novel potential diagnostic and prognostic biomarker for HCC.
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http://dx.doi.org/10.1038/s41420-021-00413-3DOI Listing
March 2021

Exposure to SARS-CoV-2 generates T-cell memory in the absence of a detectable viral infection.

Nat Commun 2021 03 19;12(1):1724. Epub 2021 Mar 19.

State Key Laboratory of Respiratory Disease & National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.

T-cell immunity is important for recovery from COVID-19 and provides heightened immunity for re-infection. However, little is known about the SARS-CoV-2-specific T-cell immunity in virus-exposed individuals. Here we report virus-specific CD4 and CD8 T-cell memory in recovered COVID-19 patients and close contacts. We also demonstrate the size and quality of the memory T-cell pool of COVID-19 patients are larger and better than those of close contacts. However, the proliferation capacity, size and quality of T-cell responses in close contacts are readily distinguishable from healthy donors, suggesting close contacts are able to gain T-cell immunity against SARS-CoV-2 despite lacking a detectable infection. Additionally, asymptomatic and symptomatic COVID-19 patients contain similar levels of SARS-CoV-2-specific T-cell memory. Overall, this study demonstrates the versatility and potential of memory T cells from COVID-19 patients and close contacts, which may be important for host protection.
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http://dx.doi.org/10.1038/s41467-021-22036-zDOI Listing
March 2021

Dysregulation of miR-23b-5p promotes cell proliferation via targeting FOXM1 in hepatocellular carcinoma.

Cell Death Discov 2021 Mar 15;7(1):47. Epub 2021 Mar 15.

Hepatobiliary Center, The First Affiliated Hospital of Nanjing Medical University; Key Laboratory of Liver Transplantation, Chinese Academy of Medical Sciences, NHC Key Laboratory of Liver Transplantation, Nanjing, China.

Growing evidence demonstrates that MicroRNAs (miRNAs) play an essential role in contributing to tumor development and progression. However, the underlying role and mechanisms of miR-23b-5p in hepatocellular carcinoma (HCC) formation remain unclear. Our study showed that miR-23b-5p was downregulated in the HCC tissues and cell lines, and lower expression of miR-23b-5p was associated with more severe tumor size and poorer survival. Gain- or loss-of-function assays demonstrated that miR-23b-5p induced G0/G1 cell cycle arrest and inhibited cell proliferation both in vitro and in vivo. qRT-PCR, western blot and luciferase assays verified that Mammalian transcription factor Forkhead Box M1 (FOXM1), upregulated in HCC specimens, was negatively correlated with miR-23b-5p expression and acted as a direct downstream target of miR-23b-5p. In addition, miR-23b-5p could regulate cyclin D1 and c-MYC expression by directly targeting FOXM1. Further study revealed that restoration of FOXM1 neutralized the cell cycle arrest and cell proliferation inhibition caused by miR-23b-5p. Taken together, our findings suggest that miR-23b-5p acted as a tumor suppressor role in HCC progression by targeting FOXM1 and may serve as a potential novel biomarker for HCC diagnosis and prognosis.
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http://dx.doi.org/10.1038/s41420-021-00440-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960996PMC
March 2021

Linderaggrenolides A-N, Oxygen-Conjugated Sesquiterpenoid Dimers from the Roots of .

ACS Omega 2021 Mar 16;6(8):5898-5909. Epub 2021 Feb 16.

State Key Laboratory of Quality Research in Chinese Medicine, Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Disease, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau 999078, People's Republic of China.

Linderaggrenolides A-N (), 14 new lindenane sesquiterpenoid dimers with oxygen bridges were isolated from the roots of . Their structures were elucidated on the basis of comprehensive spectroscopic data analysis, with the absolute configurations established by empirical approaches, electronic circular dichroism calculations, and X-ray crystallography. Compounds and were found to exhibit significant transforming growth factor-β inhibitory activity, with IC values of 25.91 and 21.52 μM, respectively.
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http://dx.doi.org/10.1021/acsomega.0c06349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931382PMC
March 2021

Neoagarooligosaccharide Protects against Hepatic Fibrosis via Inhibition of TGF-β/Smad Signaling Pathway.

Int J Mol Sci 2021 Feb 18;22(4). Epub 2021 Feb 18.

College of Pharmacy, Chosun University, Seoseok-dong, Gwangju 61452, Korea.

Hepatic fibrosis occurs when liver tissue becomes scarred from repetitive liver injury and inflammatory responses; it can progress to cirrhosis and eventually to hepatocellular carcinoma. Previously, we reported that neoagarooligosaccharides (NAOs), produced by the hydrolysis of agar by β-agarases, have hepatoprotective effects against acetaminophen overdose-induced acute liver injury. However, the effect of NAOs on chronic liver injury, including hepatic fibrosis, has not yet been elucidated. Therefore, we examined whether NAOs protect against fibrogenesis in vitro and in vivo. NAOs ameliorated PAI-1, α-SMA, CTGF and fibronectin protein expression and decreased mRNA levels of fibrogenic genes in TGF-β-treated LX-2 cells. Furthermore, downstream of TGF-β, the Smad signaling pathway was inhibited by NAOs in LX-2 cells. Treatment with NAOs diminished the severity of hepatic injury, as evidenced by reduction in serum alanine aminotransferase and aspartate aminotransferase levels, in carbon tetrachloride (CCl)-induced liver fibrosis mouse models. Moreover, NAOs markedly blocked histopathological changes and collagen accumulation, as shown by H&E and Sirius red staining, respectively. Finally, NAOs antagonized the CCl-induced upregulation of the protein and mRNA levels of fibrogenic genes in the liver. In conclusion, our findings suggest that NAOs may be a promising candidate for the prevention and treatment of chronic liver injury via inhibition of the TGF-β/Smad signaling pathway.
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http://dx.doi.org/10.3390/ijms22042041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922480PMC
February 2021

Knowledge-Guided "Community Network" Analysis Reveals the Functional Modules and Candidate Targets in Non-Small-Cell Lung Cancer.

Cells 2021 Feb 16;10(2). Epub 2021 Feb 16.

Center for Systems Biology, Department of Bioinformatics, School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China.

Non-small-cell lung cancer (NSCLC) represents a heterogeneous group of malignancies that are the leading cause of cancer-related death worldwide. Although many NSCLC-related genes and pathways have been identified, there remains an urgent need to mechanistically understand how these genes and pathways drive NSCLC. Here, we propose a knowledge-guided and network-based integration method, called the node and edge Prioritization-based Community Analysis, to identify functional modules and their candidate targets in NSCLC. The protein-protein interaction network was prioritized by performing a random walk with restart algorithm based on NSCLC seed genes and the integrating edge weights, and then a "community network" was constructed by combining Girvan-Newman and Label Propagation algorithms. This systems biology analysis revealed that the -mediated network in the largest community provides a modular biomarker, the second community serves as a drug regulatory module, and the two are connected by some contextual signaling motifs. Moreover, integrating structural information into the signaling network suggested novel protein-protein interactions with therapeutic significance, such as interactions between and , , and . This study provides new mechanistic insights into the landscape of cellular functions in the context of modular networks and will help in developing therapeutic targets for NSCLC.
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http://dx.doi.org/10.3390/cells10020402DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7919838PMC
February 2021

Prevalence and diagnosis experience of osteoporosis in postmenopausal women over 50: Focusing on socioeconomic factors.

PLoS One 2021 2;16(3):e0248020. Epub 2021 Mar 2.

Department of Orthopedics, Pusan National University Yangsan Hospital, Yangsan, Republic of Korea.

Osteoporosis is the most common disease of the musculoskeletal system in old age. Therefore, research on osteoporosis risk factors is actively being conducted. However, whether socioeconomic inequality is associated with the prevalence and diagnosis experience of osteoporosis remains largely unexplored. This study aims to investigate whether socioeconomic inequality can be a risk factor for osteoporosis in postmenopausal women. Cross-sectional data of 1,477 postmenopausal women aged over 50 obtained from the Korea National Health and Nutrition Examination Survey V-2 were analyzed. Univariate analyses were performed to calculate the prevalence of osteoporosis and the rate of osteoporosis diagnosis experience according to the risk factor categories. Multivariate logistic regression analysis was performed to identify the independent variables' associations with osteoporosis prevalence and diagnosis experience. The prevalence of osteoporosis was 34.8%, while the diagnosis experience rate was 22.1%. The higher the age, the higher the probability of osteoporosis presence and diagnosis experience. The lowest household income level was associated with a 1.63 times higher risk of osteoporosis. On the contrary, this factor was not significant for diagnosis experience. These results were similar for the 50-59 and 60-69 age groups. Among postmenopausal women, those who are older and have low socioeconomic levels are at a high risk of developing osteoporosis. Moreover, the lower the socioeconomic level, the lower the awareness of osteoporosis. Therefore, there is a need to develop more proactive preventive measures in postmenopausal women with low socioeconomic levels.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0248020PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924764PMC
March 2021

Effect of temperature and pH on the gelation, rheology, texture, and structural properties of whey protein and sugar gels based on Maillard reaction.

J Food Sci 2021 Mar 1. Epub 2021 Mar 1.

College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu, 225127, China.

This study aimed to determine the effect of initial pH and temperature on whey protein gel formation via the Maillard reaction, including changes in gel structure, rheological and texture properties. The color changes in the whey protein and glucose gels were not significant with increasing heat temperature. High temperature and alkaline conditions promoted exposure to hydrophobic groups such as -SH, which accelerated protein aggregation and gel formation. Moreover, the increased particle size and additional hydrophobic groups contributed to higher elastic modulus (G') in the whey protein gel. Fluorescence measurements revealed that more tryptophan on the protein surface decreased with increasing temperature, which indicated that exposure to tryptophan could increase the hydrophobicity of the protein gels. Whey proteins formed stronger, gummier, more elastic, and more cohesive gels at 70 ℃ under initial pH 9 conditions, which also increased with the addition of fructose.
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http://dx.doi.org/10.1111/1750-3841.15659DOI Listing
March 2021

Correlates of psychological resilience and risk: Prospective associations of self-reported and relative resilience with Connor-Davidson resilience scale, heart rate variability, and mental health indices.

Brain Behav 2021 Feb 27:e02091. Epub 2021 Feb 27.

Department of Preventive Medicine, Yonsei University College of Medicine, Seoul, South Korea.

Background: There are several ways to determine psychological resilience. However, the correlation between each measurement is not clear. We explored associations of baseline relative "resilience" and risk with later self-reported trait resilience and other biological/mental health indices.

Methods: We utilized baseline and follow-up survey data from 500 participants aged 30-64 in the community cohort. Baseline "relative" resilience was defined by: (a) negative life events (NLEs) in the six months before baseline and (b) depressive symptoms at baseline, yielding four groups of individuals: i) "Unexposed and well," "Vulnerable (depression)," "Reactive (depression)," and "Resilient." "Trait" resilience at follow-up was self-reported using the Connor-Davidson Resilience Scale (CD-RISC). Associations between relative resilience at baseline, CD-RISC, and heart rate variability (HRV) indices at follow-up were assessed with generalized linear regression models after adjustments. Associations between baseline resilience and subsequent loneliness/depression indices were also evaluated.

Results: Overall trait resilience and its subfactors at follow-up showed strong negative associations with "Reactive" at baseline (adj-β for total CD-RISC score: -11.204 (men), -9.472 (women)). However, resilience at baseline was not associated with later HRV, which was compared with the significant positive association observed between CD-RISC and HRV at the same follow-up time point. The "Reactive" exhibited significantly increased depressive symptoms at follow-up. The overall distribution pattern of CD-RISC subfactors differed by baseline resilience status by sex.

Conclusions: The "relative" resilience based on the absence of depression despite prior adversity seems to be highly related with trait resilience at follow-up but not with HRV. The sub-factor pattern of CD-RISC was different by sex.
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http://dx.doi.org/10.1002/brb3.2091DOI Listing
February 2021

Non-classical cardenolides from Calotropis gigantea exhibit anticancer effect as HIF-1 inhibitors.

Bioorg Chem 2021 Apr 18;109:104740. Epub 2021 Feb 18.

State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau 999078, People's Republic of China; Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Disease (Macau University of Science and Technology), Macau 999078, People's Republic of China. Electronic address:

Six new non-classical cardenolides (1-6), and seventeen known ones (7-23) were isolated from Calotropis gigantea. All cardenolides showed inhibitory effect on hypoxia inducible factor-1 (HIF-1) transcriptional activity with IC of 8.85 nM-16.69 µM except 5 and 7. The novel 19-dihydrocalotoxin (1) exhibited a comparable HIF-1 inhibitory activity (IC of 139.57 nM) to digoxin (IC of 145.77 nM), a well-studied HIF-1 inhibitor, and 11, 12, 14, 16 and 19 presented 1.4-15.4 folds stronger HIF-1 inhibition than digoxin. 1 and 11 showed a dose-dependent inhibition on HIF-1α protein, which led to their HIF-1 suppressing effects. Compared with LO2 and H9c2 normal cell lines, both 1 and 11 showed selective cytotoxicity against various cancer cell lines including HCT116, HeLa, HepG2, A549, MCF-7, A2780 and MDA-MB-231. Moreover, a comprehensive structure-activity relationship was concluded for these non-classical cardenolides as HIF-1 inhibitors, which may shed some light on the rational design and development of cardenolide-based anticancer drugs.
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http://dx.doi.org/10.1016/j.bioorg.2021.104740DOI Listing
April 2021

A novel host of MCR-5 belonging to Enterobacter spp. isolated from hospital sewage water.

Environ Microbiol Rep 2021 Apr 17;13(2):234-237. Epub 2021 Feb 17.

Shenzhen Institute of Respiratory Diseases, Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, the First Affiliated Hospital (Shenzhen People's Hospital), Southern University of Science and Technology, Shenzhen, China.

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http://dx.doi.org/10.1111/1758-2229.12937DOI Listing
April 2021

Mapping ticks and tick-borne pathogens in China.

Nat Commun 2021 02 17;12(1):1075. Epub 2021 Feb 17.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, P.R. China.

Understanding ecological niches of major tick species and prevalent tick-borne pathogens is crucial for efficient surveillance and control of tick-borne diseases. Here we provide an up-to-date review on the spatial distributions of ticks and tick-borne pathogens in China. We map at the county level 124 tick species, 103 tick-borne agents, and human cases infected with 29 species (subspecies) of tick-borne pathogens that were reported in China during 1950-2018. Haemaphysalis longicornis is found to harbor the highest variety of tick-borne agents, followed by Ixodes persulcatus, Dermacentor nutalli and Rhipicephalus microplus. Using a machine learning algorithm, we assess ecoclimatic and socioenvironmental drivers for the distributions of 19 predominant vector ticks and two tick-borne pathogens associated with the highest disease burden. The model-predicted suitable habitats for the 19 tick species are 14‒476% larger in size than the geographic areas where these species were detected, indicating severe under-detection. Tick species harboring pathogens of imminent threats to public health should be prioritized for more active field surveillance.
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http://dx.doi.org/10.1038/s41467-021-21375-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889899PMC
February 2021

Network-Based Target Prioritization and Drug Candidate Identification for Multiple Sclerosis: From Analyzing "Omics Data" to Druggability Simulations.

ACS Chem Neurosci 2021 Mar 10;12(5):917-929. Epub 2021 Feb 10.

Center for Systems Biology, Department of Bioinformatics, School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China.

Multiple sclerosis (MS) is the most common chronic inflammatory demyelinating disease of the central nervous system. While the drugs currently available for MS provide symptomatic benefit, there is no curative treatment. The emergence of large-scale multiomics data and network theory provide new opportunities for drug discovery in MS, as these are promising strategies for developing novel drugs. In this study, we proposed a computational framework that combined biomolecular network modeling and structural dynamics analysis to facilitate the discovery of new drugs with potential activity in MS. First, we developed a new shortest path-based algorithm that prioritized differentially expressed genes using a newly topological and functional exploration of protein-protein interaction network. Then, pathway enrichment analysis and an assessment of target druggability suggested that TNF-α-induced protein 3 (), which is involved in NF-κ B signaling, could be a potential therapeutic target for MS. Finally, druggability simulations and mutation enrichment analysis of the TNFAIP3 dimer presented two druggable sites. Follow-up pharmacophore model-based virtual screening of the two sites yielded 30 hit compounds with low energy scores. In summary, this novel method based on analyzing "omics data" and performing druggability simulations, is a systematic approach that unravels disease mechanisms and links them to the chemical space to develop treatments and can be applied to other complex diseases.
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http://dx.doi.org/10.1021/acschemneuro.1c00011DOI Listing
March 2021

Alkaline brain pH shift in rodent lithium-pilocarpine model of epilepsy with chronic seizures.

Brain Res 2021 May 5;1758:147345. Epub 2021 Feb 5.

Harvard Medical School, Boston, MA, 02115, USA; Division of Newborn Medicine, Dept. Pediatrics, Boston Children's Hospital, Boston, MA, 02115, USA. Electronic address:

Brain pH is thought to be important in epilepsy. The regulation of brain pH is, however, still poorly understood in animal models of chronic seizures (SZ) as well as in patients with intractable epilepsy. We used chemical exchange saturation transfer (CEST) MRI to noninvasively determine if the pH is alkaline shifted in a rodent model of the mesial temporal lobe (MTL) epilepsy with chronic SZ. Taking advantage of its high spatial resolution, we determined the pH values in specific brain regions believed to be important in this model produced by lithium-pilocarpine injection. All animals developed status epilepticus within 90 min after the lithium-pilocarpine administration, but one animal died within 24 hrs. All the surviving animals developed chronic SZ during the first 2 months. After SZ developed, brain pH was determined in the pilocarpine and control groups (n = 8 each). Epileptiform activity was documented in six pilocarpine rats with scalp EEG. The brain pH was estimated using two methods based on magnetization transfer asymmetry and amide proton transfer ratio. The pH was alkaline shifted in the pilocarpine rats (one outlier excluded) compared to the controls in the hippocampus (7.29 vs 7.17, t-test, p < 0.03) and the piriform cortex (7.34 vs. 7.06, p < 0.005), marginally more alkaline in the thalamus (7.13 vs. 7.01, p < 0.05), but not in the cerebral cortex (7.18 vs. 7.08, p > 0.05). Normalizing the brain pH may lead to an effective non-surgical method for treating intractable epilepsy as it is known that SZ can be eliminated by lowering the pH.
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http://dx.doi.org/10.1016/j.brainres.2021.147345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987840PMC
May 2021

The HS1 Strain Renders Vegetable Plants Resistant and Tolerant against Pathogen Infection and High Salinity Stress.

Plant Pathol J 2021 Feb;37(1):72-78

Department of Molecular Genetics, Dong-A University, Busan 4915, Korea.

Various management systems are being broadly employed to minimize crop yield loss resulting from abiotic and biotic stresses. Here we introduce a HS1 strain as a potent candidate for managing manifold stresses on vegetable plants. Considering 16S rDNA sequence and biochemical characteristics, the strain is closely related to . The HS1's soil-drench confers disease resistance on tomato and paprika plants against infection with and , respectively. Root and shoot growths are also increased in HS1-treated cabbage, cucumber, and tomato plants, compared with those in mock-treated plants, after application of high salinity solution. Moreover, the pretreatment of HS1 on cabbage plants inhibits the degradation of chloroplast pigments caused by high salinity stresses, whereas the inhibitory effect is not observed in cucumber plants. These findings suggest that HS1 stain inhibits disease development and confers tolerance to salinity stress on vegetable plants.
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http://dx.doi.org/10.5423/PPJ.NT.12.2020.0219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847759PMC
February 2021

Probing Single-Atom Catalysts and Catalytic Reaction Processes by Shell-Isolated Nanoparticle-Enhanced Raman Spectroscopy.

Angew Chem Int Ed Engl 2021 Apr 17;60(17):9306-9310. Epub 2021 Mar 17.

College of Materials, State Key Laboratory of Physical Chemistry of Solid Surfaces,iChEM, College of Chemistry and Chemical Engineering, Fujian Key Laboratory of Advanced Materials, College of Energy, Xiamen University, Xiamen, 361005, China.

Developing advanced characterization techniques for single-atom catalysts (SACs) is of great significance to identify their structural and catalytic properties. Raman spectroscopy can provide molecular structure information, and thus, the technique is a promising tool for catalysis. However, its application in SACs remains a great challenge because of its low sensitivity. We develop a highly sensitive strategy that achieves the characterization of the structure of SACs and in situ monitoring of the catalytic reaction processes on them by shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS) for the first time. Using the strategy, Pd SACs on different supports were identified by Raman spectroscopy and the nucleation process of Pd species from single atoms to nanoparticles was revealed. Moreover, the catalytic reaction processes of the hydrogenation of nitro compounds on Pd SACs were monitored in situ, and molecular insights were obtained to uncover the unique catalytic properties of SACs. This work provides a new spectroscopic tool for the in situ study of SACs, especially at solid-liquid interfaces.
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http://dx.doi.org/10.1002/anie.202100198DOI Listing
April 2021

Interspecific Variation in the Unsaturation Level of Seed Oils Were Associated With the Expression Pattern Shifts of Duplicated Desaturase Genes and the Potential Role of Other Regulatory Genes.

Front Plant Sci 2020 13;11:616338. Epub 2021 Jan 13.

Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Eco-Chongming (IEC), Fudan University, Shanghai, China.

Seed oils are of great economic importance both for human consumption and industrial applications. The nutritional quality and industrial value of seed oils are mostly determined by their fatty acid profiles, especially the relative proportions of unsaturated fatty acids. Tree peony seed oils have recently been recognized as novel edible oils enriched in α-linolenic acid (ALA). However, congeneric species, such as and , showed marked variation in the relative proportions of different unsaturated fatty acids. By comparing the dynamics of fatty acid accumulation and the time-course gene expression patterns between and , we identified genes that were differentially expressed between two species in developing seeds, and showed congruent patterns of variation between expression levels and phenotypes. In addition to the well-known desaturase and acyltransferase genes associated with fatty acid desaturation, among them were some genes that were conservatively co-expressed with the desaturation pathway genes across phylogenetically distant ALA-rich species, including and . Go enrichment analysis revealed that these genes were mainly involved in transcriptional regulation, protein post-translational modification and hormone biosynthesis and response, suggesting that the fatty acid synthesis and desaturation pathway might be subject to multiple levels of regulation.
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http://dx.doi.org/10.3389/fpls.2020.616338DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838364PMC
January 2021

The effect of baseline serum uric acid on chronic kidney disease in normotensive, normoglycemic, and non-obese individuals: A health checkup cohort study.

PLoS One 2021 27;16(1):e0244106. Epub 2021 Jan 27.

Division of Nephrology, Department of Internal Medicine, Korea University Anam Hospital, Korea University College of Medicine, Seoul, Republic of Korea.

Introduction: The independent role of serum uric acid (SUA) on kidney disease is controversial due to its association with metabolic syndrome. The objective of this study was to investigate the association of baseline SUA with development of chronic kidney disease and eGFR decline in normotensive, normoglycemic and non-obese individuals during follow up period.

Materials And Methods: We included non-hypertensitive, non-diabetic, and non-obese 13,133 adults with estimated glomerular filtration rate (eGFR) ≥ 60ml/min/1.73m2 who had a voluntary health check-up during 2004-2017.

Results: SUA was positively related to adjusted means of systolic blood pressure (SBP), triglyceride, body mass index, and body fat percent. SUA was inversely associated with high density lipoprotein HDL (P for trend ≤0.001). SUA was an independent risk factor for the development of diabetes, hypertension, and obesity. During 45.0 [24.0-76.0] months of median follow up, the highest quartiles of SUA showed significant risks of 30% eGFR decline compared than the lowest quartile (RR:3.701; 95% CI: 1.504-9.108). The highest quartile had a 2.2 fold (95% CI: 1.182-4.177) increase in risk for incident chronic kidney disease (CKD).

Conclusions: SUA is an independent risk factor for the development of diabetes, hypertension, and obesity in the healthy population. High SUA is associated with increased risk of CKD development and eGFR decline in participants with intact renal function.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0244106PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840038PMC
January 2021

A genome-wide investigation of the effect of farming and human-mediated introduction on the ubiquitous seaweed Undaria pinnatifida.

Nat Ecol Evol 2021 03 25;5(3):360-368. Epub 2021 Jan 25.

Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea.

Human activity is an important driver of ecological and evolutionary change on our planet. In particular, domestication and biological introductions have important and long-lasting effects on species' genomic architecture and diversity. However, genome-wide analysis of independent domestication and introduction events within a single species has not previously been performed. The Pacific kelp Undaria pinnatifida provides such an opportunity because it has been cultivated in its native range in Northeast Asia but also introduced to four other continents in the past 50 years. Here we present the results of a genome-wide analysis of natural, cultivated and introduced populations of U. pinnatifida to elucidate human-driven evolutionary change. We demonstrate that these three categories of origin can be distinguished at the genome level, reflecting the combined influence of neutral (demography and migration) and non-neutral (selection) processes.
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http://dx.doi.org/10.1038/s41559-020-01378-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7929912PMC
March 2021

Discrepancies in periodontitis classification among dental practitioners with different educational backgrounds.

BMC Oral Health 2021 Jan 22;21(1):39. Epub 2021 Jan 22.

Department of Periodontics, School of Dentistry, Loma Linda University, Loma Linda, CA, USA.

Background: The 2018 classification of periodontal disease characterizes the disease with a multidimensional staging and grading system. The purpose of this multicenter study was to examine variations in periodontitis classification among dental practitioners with different postgraduate educational backgrounds at the University of Maryland School of Dentistry and the Loma Linda University School of Dentistry using the 2018 classification.

Methods: This cross-sectional observational study included two cohorts: dental practitioners with periodontal backgrounds (n = 31) and those with other educational backgrounds (n = 33). The survey instrument contained three periodontitis cases presented with the guideline of the 2018 classification and a questionnaire including closed and open-ended questions. The participants were asked to review each case and to fill out the questionnaire independently. Fisher's exact test was conducted to examine the differences in responses between the two cohorts. Polychoric correlations were calculated to examine the relation between the level of familiarity with the 2018 classification and the accuracy of the classification.

Results: The distribution of item responses was significantly different between the two cohorts regarding only one item, grading for Case 1 (p = 0.01). No significant differences in accuracy between the two cohorts were observed except for two items, grading in Case 1 (p = 0.03) and staging in Case 3 (p = 0.04). There were no significant differences in risk factor identification for each case among the two cohorts (p = 1.00, Case 1; p = 0.22, Case 2). Staging in Case 3 ([Formula: see text] = 0.52) and risk factor identification in Case 2 ([Formula: see text]= 0.32) were significantly correlated with familiarity with the 2018 classification.

Conclusion: A fair level of agreement in periodontitis classification was observed among dental practitioners with different educational backgrounds when the 2018 classification was used. The periodontal cohort showed better agreement levels and partially better accuracy. Risk factor identification for periodontal disease was difficult regardless of the educational background.
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http://dx.doi.org/10.1186/s12903-020-01371-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821642PMC
January 2021

The impact of writing on academic performance for medical students.

BMC Med Educ 2021 Jan 19;21(1):61. Epub 2021 Jan 19.

Department of Psychology, Seoul National University, Seoul, South Korea.

Background: Writing is a useful learning activity that promotes higher-order thinking, but there are limited studies that prove its effectiveness. In previous research, researchers tested the effect of summary writing on students' comprehension and found no significant difference from that of re-studying texts. The purpose of this study, therefore, is to expand previous findings and investigate the effect of two types of writing tasks on medical students' academic performance, specifically in the transfer of knowledge.

Methods: An experiment was conducted with 139 medical students from Seoul National University College of Medicine. They were randomly assigned to three study conditions: self-study (SS), expository writing (EW), and argumentative writing (AW) group. Each group studied the given material by the method they were assigned, and they were tested on their comprehension and transfer of knowledge using rote-memory type items and transfer type items respectively.

Results: The results showed that the two writing groups displayed better performance than the SS group in transfer type items, while there was no difference in scores between the EW and AW group. However, the three groups showed no significant difference in their scores for rote-memory type items. Also, there was a positive correlation between the writing scores and transfer type item scores in the AW group.

Conclusions: This study provides empirical evidence for writing to be adopted in medical education for greater educational benefits. Our findings indicate that writing can enhance learning and higher-order thinking, which are critical for medical students.
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http://dx.doi.org/10.1186/s12909-021-02485-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814462PMC
January 2021

SO Resisting Pd-doped Pr Ce MnO Perovskites for Efficient Denitration at Low Temperature.

Chem Asian J 2021 Mar 28;16(5):530-537. Epub 2021 Jan 28.

School of Resources and Environmental Engineering, State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, P. R. China.

H -SCR is served as the promising technology for the controlling of NO emission, and the Pd-based derivative catalyst exhibited high NO reduction performance. Effectively regulating the electronic configuration of the active component is favorable to the rational optimization of noble Pd. In this work, a series of Pr Ce Mn Pd O @Ni were successfully synthesized and exhibited superior NO conversion efficiency at low temperatures. 92.7 % conversion efficiency was achieved at 200 °C over Pr Ce Mn Pd O @Ni in the presence of 4 % O with a GHSV of 32000 h . Meanwhile, the outstanding performance was obtained in the resistance to SO (200 ppm) and H O (8 %). Deduced from the results of XRD, Raman, XPS, and H -TPR, the modification of d orbit states in palladium was confirmed originating from the incorporation in the B site of Pr Ce Mn Pd O . The existence of higher valence (Pd and Pd ) than the bivalence in Pr Ce Mn Pd O catalyst was evidenced by XPS analysis. Our research provides a new sight into the H -SCR through the higher utilization of Pd.
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http://dx.doi.org/10.1002/asia.202001426DOI Listing
March 2021

Efficient Palladium-Catalyzed Carbonylation of 1,3-Dienes: Selective Synthesis of Adipates and Other Aliphatic Diesters.

Angew Chem Int Ed Engl 2021 Apr 3;60(17):9527-9533. Epub 2021 Mar 3.

Leibniz-Institut für Katalyse e. V., Albert-Einstein-Straße 29a, 18059, Rostock, Germany.

The dicarbonylation of 1,3-butadiene to adipic acid derivatives offers the potential for a more cost-efficient and environmentally benign industrial process. However, the complex reaction network of regioisomeric carbonylation and isomerization pathways, make a selective and direct transformation particularly difficult. Here, we report surprising solvent effects on this palladium-catalysed process in the presence of 1,2-bis-di-tert-butylphosphin-oxylene (dtbpx) ligands, which allow adipate diester formation from 1,3-butadiene, carbon monoxide, and methanol with 97 % selectivity and 100 % atom-economy under scalable conditions. Under optimal conditions a variety of di- and triesters from 1,2- and 1,3-dienes can be obtained in good to excellent yields.
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http://dx.doi.org/10.1002/anie.202015329DOI Listing
April 2021

Cross-sectional analysis of the association between serum uric acid levels and handgrip strength among Chinese adults over 45 years of age.

Ann Transl Med 2020 Dec;8(23):1562

Zhejiang Provincial Key Laboratory for Diagnosis and Treatment of Aging and Physic-chemical Injury Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Background: Sarcopenia is the decline in muscle strength and mass attributed to aging. The pathogenesis of sarcopenia may be triggered by oxidative stress; uric acid (UA) has strong antioxidant properties. This study aimed to examine if the serum UA level is associated with handgrip strength (HGS), which is a useful indicator of sarcopenia among Chinese participants aged over 45.

Methods: Our study included 992 eligible participants (583 males and 409 females). Based on serum UA quartiles and gender, the participants were divided into 8 groups. HGS was measured in kilograms using an electronic dynamometer. Face-to-face visits and fasting blood analyses were performed to determine the serum UA levels and various covariates. Univariate analysis of variance (ANOVA) and covariance (ANCOVA) was conducted to analyze the linear or quadratic trend between the UA levels and grip strength.

Results: Participants were grouped according to UA quartiles by gender. In both genders, ANOVA showed an inverted J-shaped association between serum UA levels and HGS (P for quadratic trend =0.004 in men, P for quadratic trend =0.003 in women). After adjusting for potential confounders, the association between the UA quartiles and HGS was unchanged, irrespective of gender.

Conclusions: The results suggest that a specific range of serum UA levels may be associated with better HGS among Chinese adults aged over 45.
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http://dx.doi.org/10.21037/atm-20-2813aDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791196PMC
December 2020