Publications by authors named "Ji Li"

1,492 Publications

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Overexpression of PLK1 relieved the myocardial ischemia-reperfusion injury of rats through inducing the mitophagy and regulating the p-AMPK/FUNDC1 axis.

Bioengineered 2021 Dec;12(1):2676-2687

Department of Cardiovascular Internal Medicine, The Second Affiliated Hospital of Guizhou Medical University, Kaili City, Guizhou Province, China.

Myocardial cell injury caused by myocardial ischemia and reperfusion is one of the main causes of the occurrence and development of heart disease. Recent study has shown that inducing mitophagy of cardiomyocytes is a crucial method to alleviate ischemia-reperfusion injury. While, Polo-like kinase 1 (PLK1) can induce the mitophagy of breast cancer cells. Moreover, PLK1 was able to promote the expression of p-AMPK and FUNDC1, which are the protective factors for myocardium. Therefore, the mouse model of ischemia/reperfusion was established and the effect of PLK1 on ischemia reperfusion induced myocardial damage was investigated. The PLK1 was overexpressed in H9c2 cells and rat model of ischemia/reperfusion. Ischemia reperfusion inhibited the expression of PLK1. While overexpression of PLK1 relieved the myocardial infarction and myocardium apoptosis through inducing mitophagy in rats model of ischemia reperfusion. In vitro, the H9c2 cells overexpressing the PLK1 were treated with the hypoxia and reoxygenation and the apoptosis, survival rate and expression of mitophagy-related proteins of H9c2 cells were detected using the flow cytometry, CCK-8 assay and western blotting. The results reveled that overexpression of PLK1 alleviated the hypoxia and reoxygenation induced apoptosis of H9c2 cells and promoted the expression of mitophagy-related proteins. In addition, enhanced PLK1 expression promoted the expression of p-AMPK and FUNDC1 in H9c2 cells. However, the inhibition of FUNDC1 abolished the positive effect of PLK1 on H9c2 cells mentioned above. In conclusion, PLK1 alleviated the ischemia reperfusion induced myocardial damage by inducing the mitophagy in a p-AMPK/FUNDC1 signaling dependent pathway.
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http://dx.doi.org/10.1080/21655979.2021.1938500DOI Listing
December 2021

Lung Ultrasound Score as a Predictor of Mortality in Patients With COVID-19.

Front Cardiovasc Med 2021 25;8:633539. Epub 2021 May 25.

Department of Ultrasound Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Lung injury is a common condition among hospitalized patients with coronavirus disease 2019 (COVID-19). However, whether lung ultrasound (LUS) score predicts all-cause mortality in patients with COVID-19 is unknown. The aim of the present study was to explore the predictive value of lung ultrasound score for mortality in patients with COVID-19. Patients with COVID-19 who underwent lung ultrasound were prospectively enrolled from three hospitals in Wuhan, China between February 2020 and March 2020. Demographic, clinical, and laboratory data were collected from digital patient records. Lung ultrasound scores were analyzed offline by two observers. Primary outcome was in-hospital mortality. Of the 402 patients, 318 (79.1%) had abnormal lung ultrasound. Compared with survivors ( = 360), non-survivors ( = 42) presented with more B2 lines, pleural line abnormalities, pulmonary consolidation, and pleural effusion (all < 0.05). Moreover, non-survivors had higher global and anterolateral lung ultrasound score than survivors. In the receiver operating characteristic analysis, areas under the curve were 0.936 and 0.913 for global and anterolateral lung ultrasound score, respectively. A cutoff value of 15 for global lung ultrasound score had a sensitivity of 92.9% and specificity of 85.3%, and 9 for anterolateral score had a sensitivity of 88.1% and specificity of 83.3% for prediction of death. Kaplan-Meier analysis showed that both global and anterolateral scores were strong predictors of death (both < 0.001). Multivariate Cox regression analysis showed that global lung ultrasound score was an independent predictor (hazard ratio, 1.08; 95% confidence interval, 1.01-1.16; = 0.03) of death together with age, male sex, C-reactive protein, and creatine kinase-myocardial band. Lung ultrasound score as a semiquantitative tool can be easily measured by bedside lung ultrasound. It is a powerful predictor of in-hospital mortality and may play a crucial role in risk stratification of patients with COVID-19.
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http://dx.doi.org/10.3389/fcvm.2021.633539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185027PMC
May 2021

Willingness-to-Pay and Benefit-Cost Analysis of Botulinum Toxin for the Treatment of Rosacea in China: Findings from a Web-Based Survey.

Patient Prefer Adherence 2021 3;15:1197-1205. Epub 2021 Jun 3.

Department of Dermatology, Xiangya Hospital, Central South University, Changsha, People's Republic of China.

Purpose: Botulinum toxin (BTX) is a new treatment approach primarily aimed at relieving flushing and erythema for rosacea, but it is expensive and lacks economic benefit evaluation studies.This study aimed to investigate willingness-to-pay (WTP) of BTX treatment and conduct benefit-cost analysis (BCA) to assess if BTX treatment for rosacea is recommendable from a viewpoint of economics in China.

Methods: WTP of BTX treatment in rosacea and information of sociodemographic and clinical characteristics were inquired via an online questionnaire among the Chinese rosacea patients. The WTP was inquired by photos of three cases with different severities of rosacea before and after BTX treatment. The benefit-cost ratio (BCR) was calculated by dividing WTP by cost. Factors associated with WTP were identified using logistic regression models.

Results: The average costs of BTX treatment were USD410.09. The mean WTP for Case 1, Case 2, and Case 3 was USD295.53, 307.91, and 311.78, respectively (p<0.05 for Case 1 vs Case 3). 44.31% to 47.52% of Chinese rosacea patients were willing to pay for the BTX treatment. The BCRs were 0.72, 0.75, and 0.76 for Case 1, Case 2, and Case 3, respectively. A positive correlation between WTP and visiting frequency in the past year (OR=1.181-1.200, p=0.015-0.032, for Cases 1 and 2) or Dermatology Life Quality Index (DLQI) score (OR=2.022-2.266, all p<0.01) was observed, but duration (OR=0.521-0.564, p<0.05, for Cases 1 and 2) of rosacea was negatively correlated with WTP.

Conclusion: For rosacea patients with poor quality of life, and those with high visiting frequency, BTX should be regarded as a recommendable new treatment in China.
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http://dx.doi.org/10.2147/PPA.S311322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185460PMC
June 2021

Pathological complete response to neoadjuvant ceritinib of a crizotinib-resistant, stage IIIB non-small cell lung cancer with ALK rearrangement: A case report.

Thorac Cancer 2021 Jun 9. Epub 2021 Jun 9.

Department of Thoracic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

The treatment of stage IIIB non-small cell lung cancer (NSCLC) is complicated, the best strategy is chosen individually and surgery is usually not recommended. A 50-year-old female was diagnosed with locally advanced lung adenocarcinoma (stage IIIB, T2bN3M0). Fluorescence in situ hybridization (FISH) analysis revealed an ALK rearrangement. Crizotinib was administered and progression was seen after five months. The patient then received ceritinib with a palliative intent, which led to downstaging (IIIA[N2]) with a radiological and metabolic response. Right lower lobe lobectomy was performed at 12 months post-surgery, and the patient is still disease-free according to the last computed tomography (CT) scan. The unintended downstaging from ceritinib provided a chance for resection in our patient who had ALK-positive stage IIIB NSCLC after the failure of first-line crizotinib, indicating potential usage of ceritinib in the neoadjuvant setting. Future perspective trials are warranted to investigate the role of ceritinib in earlier stages as a primary drug.
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http://dx.doi.org/10.1111/1759-7714.14045DOI Listing
June 2021

NAD deficit, protein acetylation and muscle aging.

Aging (Albany NY) 2021 Jun 7. Epub 2021 Jun 7.

Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN 55905, USA.

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http://dx.doi.org/10.18632/aging.203177DOI Listing
June 2021

Downregulation of lncRNA XR_429159.1 Linked to Brain Metastasis in Patients With Limited-Stage Small-Cell Lung Cancer.

Front Oncol 2021 17;11:603271. Epub 2021 May 17.

Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, China.

The purpose of this study was to identify aberrant long non-coding RNAs (lncRNAs) and explore the predictive value of lncRNA expression patterns on the risk of brain metastases (BMs) in patients with limited-stage small-cell lung cancer (SCLC). We executed an array of lncRNA and mRNA chip assays to examine the expression in peripheral blood mononuclear cells obtained from SCLC patients with BMs and compared the expression patterns against those from patients without BMs to identify lncRNAs associated with BMs. Validation was performed against clinical data to further confirm the relationship between lncRNAs and BM. Kaplan-Meier analysis was applied to estimate the cumulative incidence of BMs, and differences between the groups were analyzed using the log-rank test. The expression of 67 lncRNAs (27 upregulated and 40 downregulated) and 47 mRNAs (20 upregulated and 27 downregulated) was significantly different between the BM and non-BM groups (fold change ≥ 2.0, -value ≤ 0.05), based on the lncRNA and mRNA chip assays. Four lncRNAs were verified by quantitative real-time polymerase chain reaction (qRT-PCR) to confirm the accuracy of the microarray data, and the results of 11 patient pairs (11 patients with BM and 11 patients without BM) revealed that low LncRNA XR_429159.1 expression was a high-risk factor for BM. Further clinical data showed that the incidence of BM among 25 patients with low-level LncRNA XR_429159.1 expression was 31% at 1 year, compared with 14.3% among the 18 patients with high-level LncRNA XR_429159.1 expression ( = 0.035). Our present study identified the low-level expression of lncRNA XR_429159.1 as a high-risk factor among BM in patients with limited-stage SCLC. LncRNA XR_429159.1 is a critical molecule that regulates SCLC metastasis, involved in the neuroepithelial transforming gene 1 (NET1) pathway, and serum levels of this lncRNA are significantly associated with the risk of BM.
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http://dx.doi.org/10.3389/fonc.2021.603271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166247PMC
May 2021

Aberrant modulations of static functional connectivity and dynamic functional network connectivity in chronic migraine.

Quant Imaging Med Surg 2021 Jun;11(6):2253-2264

Department of Integrated Traditional and Western Medicine, Huashan Hospital, Fudan University, Shanghai, China.

Background: Chronic migraine (CM) is a common and disabling neurological disorder that affects 1-2% of the global population. The aim of the present study was to identify the functional characteristics of the CM brain using static functional connectivity (s-FC), static functional network connectivity (s-FNC), and dynamic functional network connectivity (d-FNC) analyses.

Methods: In the present study, 17 CM patients and 20 sex- and age-matched healthy controls (HCs) underwent resting-state functional magnetic resonance imaging. We utilized independent component (IC) analysis to identify 13 ICs. These 13 ICs were then classified into the following 6 resting-state networks (RSNs): the default mode network (DMN), executive control network (ECN), dorsal attention network, auditory network (AN), visual network (VN), and cerebellum network. Subsequently, s-FC, s-FNC, and d-FNC analyses of 13 ICs were employed for between-group comparisons. Three temporal metrics (fraction of time spent, mean dwell time, and number of transitions), which were derived from the state-transition vector, were calculated for group comparisons. In addition, correlation analyses were performed between these dynamic metrics and clinical characteristics [mean visual analog scale (VAS) scores, days with headache per month, days with migraine pain feature per month, and disease duration].

Results: In the comparison of s-FC of 13 ICs within RSNs between the CM and HC groups, increased connectivity was observed in the left angular gyrus (Angular_L) of the ECN (IC 2) and the right superior parietal gyrus (Parietal_Sup_R) of the AN (IC 5), and reduced connectivity was found in the left superior frontal gyrus (Frontal_Sup_2_L) of the AN (IC 5) and DMN (IC 19), the right calcarine sulcus (Calcarine_R) of the VN (IC 7), and the left precuneus (Precuneus_L) of the DMN (IC 17) in CM patients. In the comparison of the d-FNC of 13 IC pairs within RSNs between the two groups, the CM group exhibited significantly decreased connections between the DMN (IC 11) and AN (IC 5), and increased connections between the ECN (IC 2, IC 4) and DMN (IC 19), ECN (IC 4) and AN (IC 5), and ECN (IC 4) and VN (IC 13) in state 1. However, no significant differences in s-FNC were observed between the two groups during the s-FNC analysis. Between-group comparisons of three dynamic metrics between the CM and HC groups showed a longer fraction of time spent and mean dwell time in state 2 for CM patients. Furthermore, from the correlation analyses between these metrics and clinical characteristics, we observed a significant positive correlation between the number of transitions and mean VAS scores.

Conclusions: Our findings suggest that functional features of the CM brain may fluctuate over time instead of remaining static, and provide further evidence that migraine chronification may be related to abnormal pattern connectivity between sensory and cognitive brain networks.
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http://dx.doi.org/10.21037/qims-20-588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107335PMC
June 2021

Variations in Gut Microbiome are Associated with Prognosis of Hypertriglyceridemia-Associated Acute Pancreatitis.

Biomolecules 2021 May 6;11(5). Epub 2021 May 6.

Department of Gastroenterology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.

Hypertriglyceridemia-associated acute pancreatitis (HTGAP) is linked with increased severity and morbidity. Intestinal flora plays an important role in the progression of acute pancreatitis (AP). However, pathogenetic association between gut microbiota and HTGAP remains unknown. In this study, we enrolled 30 HTGAP patients and 30 patients with AP that is evoked by other causes. The V3-V4 regions of 16S rRNA sequences of the gut microbiota were analyzed. Clinical characteristics, microbial diversity, taxonomic profile, microbiome composition, microbiological phenotype, and functional pathways were compared between the two groups. Our results showed that the HTGAP group had a higher proportion of severe AP (46.7% vs. 20.0%), organ failure (56.7% vs. 30.0%), and a longer hospital stay (18.0 days vs. 6.5 days). HTGAP group also had poorer microbial diversity, higher abundances of and , but lower abundances of , , and as compared with non-HTGAP group. Correlation analysis revealed that gut bacterial taxonomic and functional changes were linked with local and systemic complications, ICU admission, and mortality. This study revealed that alterations of gut microbiota were associated with disease severity and poor prognosis in HTGAP patients, indicating a potential pathophysiological link between gut microbiota and hypertriglyceridemia related acute pancreatitis.
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http://dx.doi.org/10.3390/biom11050695DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148198PMC
May 2021

Charge-switchable nanoparticles enhance Cancer immunotherapy based on mitochondrial dynamic regulation and immunogenic cell death induction.

J Control Release 2021 May 29;335:320-332. Epub 2021 May 29.

Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Shenyang, 110016, PR China. Electronic address:

Cancer immunotherapy has emerged as a promising option for various malignant tumors therapy. Unfortunately, the existence of an immunosuppressive tumor microenvironment (ITM) and the absence of an effective delivery strategy limit its further application. To reverse the ITM and exploit a favorable delivery system for cancer immunotherapy, twin-like charge-switchable nanoparticles (shMFN1-NPs + DOX-NPs, termed as MIX-NPs) were developed to selectively target tumor-associated macrophages (TAMs) and cancer cells, respectively. The shMFN1-NPs (150 nm) and DOX-NPs (160 nm) both had uniform spherical-shaped structures and showed favorable tumor tissue accumulation. Based on the pH-responsive core-shell separation, the nanoparticles obtained an excellent balance between the circulation time and cellular uptake. Mitochondrial dynamics are involved in macrophage polarization by regulating a novel signaling network, involving the modulation from fusion (M2-TAMs) to mitochondrial fission (M1-TAMs). M2-TAMs targeting nanoparticles shMFN1-NPs were fabricated to deliver shMFN1 for repolarization of TAMs from the M2 to M1 phenotype by inhibiting mitochondrial fusion. Moreover, DOX-NPs effectively triggered the immunogenic cell death (ICD) of cancer cells, and the succeeding maturation of dendritic cells (DCs) promoted the infiltration and activation of CD8 T cells. MIX-NPs displayed the strongest antitumor efficacy (TIR = 83%) in the subcutaneous 4T1 tumor model. MIX-NPs suppressed the myeloid-derived suppressor cells (MDSCs) and regulatory T lymphocytes (Tregs) to further remodel the ITM. Taken together, our developed drug delivery strategy reversed the ITM and activated the antitumor immune response, providing a profound prospective treatment strategy in cancer immunotherapy.
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http://dx.doi.org/10.1016/j.jconrel.2021.05.036DOI Listing
May 2021

Massive hemothorax caused by intercostal artery pseudoaneurysm:a case report.

J Cardiothorac Surg 2021 May 31;16(1):156. Epub 2021 May 31.

Department of Cardiothoracic Surgery, The First People's Hospital of Neijiang, No. 1866, West Section of Hanan Avenue, Shizhong District, Neijiang, 641000, Sichuan, China.

Background: Intercostal artery pseudoaneurysm is rare and at the risk of rupture. The aetiology is always reported to be iatrogenic and traumatic injury. Embolisation is the most common therapeutic method. Here, we report a case of spontaneous intercostal artery pseudoaneurysm and cured by combining covered stent grafting and surgical management.

Case Presentation: A 60-year-old man complained of acute right back pain for 5 h. Computed tomography showed right massive hemothorax and a giant mass with distinct feeding vessel originated from the thoracic aorta within the right hemithorax. Thoracocentesis was performed, and then a covered stent was positioned across the origin of the feeding vessel. The patient was diagnosed with intercostal artery pseudoaneurysm. Finally, we successfully resected the pseudoaneurysm and ligated the proximal part of the artery. Histologic examination have proved the diagnosis. The postoperative course was uneventful, and the patient was discharged on postoperative day 10. There is no recurrence reported during follow-up.

Conclusions: Spontaneous intercostal artery pseudoaneurysm is extremly rare. Delayed hemothorax due to rupture of the pseudoaneurysm may occur years after the formation. Early diagnosis is important and a combined treatment of endovascular intervention and surgical management is feasible, especially for the case of ruptured large tumour-like mass presentation of the pseudoaneurysm.
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http://dx.doi.org/10.1186/s13019-021-01548-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166138PMC
May 2021

Comparative analysis of latex transcriptomes reveals the potential mechanisms underlying rubber molecular weight variations between the Hevea brasiliensis clones RRIM600 and Reyan7-33-97.

BMC Plant Biol 2021 May 29;21(1):244. Epub 2021 May 29.

Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs; State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, P. R. China.

Background: The processabilities and mechanical properties of natural rubber depend greatly on its molecular weight (MW) and molecular weight distribution (MWD). However, the mechanisms underlying the regulation of molecular weight during rubber biosynthesis remain unclear.

Results: In the present study, we determined the MW and particle size of latex from 1-year-old virgin trees and 30-year-old regularly tapped trees of the Hevea clones Reyan7-33-97 and RRIM600. The results showed that both the MW and the particle size of latex varied between these two clones and increased with tree age. Latex from RRIM600 trees had a smaller average particle size than that from Reyan7-33-97 trees of the same age. In 1-year-old trees, the Reyan7-33-97 latex displayed a slightly higher MW than that of RRIM600, whereas in 30-year-old trees, the RRIM600 latex had a significantly higher MW than the Reyan7-33-97 latex. Comparative analysis of the transcriptome profiles indicated that the average rubber particle size is negatively correlated with the expression levels of rubber particle associated proteins, and that the high-MW traits of latex are closely correlated with the enhanced expression of isopentenyl pyrophosphate (IPP) monomer-generating pathway genes and downstream allylic diphosphate (APP) initiator-consuming non-rubber pathways. By bioinformatics analysis, we further identified a group of transcription factors that potentially regulate the biosynthesis of IPP.

Conclusions: Altogether, our results revealed the potential regulatory mechanisms involving gene expression variations in IPP-generating pathways and the non-rubber isoprenoid pathways, which affect the ratios and contents of IPP and APP initiators, resulting in significant rubber MW variations among same-aged trees of the Hevea clones Reyan7-33-97 and RRIM600. Our findings provide a better understanding of rubber biosynthesis and lay the foundation for genetic improvement of rubber quality in H. brasiliensis.
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http://dx.doi.org/10.1186/s12870-021-03022-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164328PMC
May 2021

Green synthesis of gold nanoparticles from and its anticancer effect on liver cancer.

Drug Deliv 2021 Dec;28(1):985-994

Department of Clinical Laboratory, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

A novel gold nanoparticle (Do-AuNP) was successfully synthesized from water extracts of traditional Chinese medicine (DO) without using any extra chemicals regents. The physicochemical properties of Do-AuNPs were analyzed by transmission electron microscopy, dynamic light scattering, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, and atomic force microscopy. The amount of DO extract on the AuNPs was about 13%. In order to evaluate the anti-tumor efficiency and biosafety, the inhibitory rate of HepG2 cells and survival rate of L02 cells were performed , and the immunohistochemical analysis of H&E, Ki-67, and TUNEL staining were conducted . Our results demonstrated that Do-AuNP had better anti-tumor efficiency compared with DO extraction alone without increasing toxicity and . The present study provides useful information for Do-AuNP as a new nanomedicine for liver cancer.
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http://dx.doi.org/10.1080/10717544.2021.1921079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171258PMC
December 2021

[Mechanisms Summary and Potential Analysis of EPS as a Flame Retardant].

Huan Jing Ke Xue 2021 Jun;42(6):2583-2594

Sino-Dutch R & D Centre for Future Wastewater Treatment Technologies, Key Laboratory of Urban Stormwater System and Water Environment, Ministry of Education, Beijing University of Civil Engineering and Architecture, Beijing 100044, China.

High value-added extracellular polymer substance (EPS) extracted from excess sludge can effectively promote resource recovery from wastewater. EPS can replace traditional alginate in the food, medicine, textile, printing and dyeing, papermaking, and household chemicals industries. Moreover, its unique performance as a flame retardant has shown attractive potential for aircraft including space shuttles. This is due to the complicated chemical structure and composition of EPS, the excellent compatibility, adhesion, and other advantages of which could yield environmental-friendly flame-retardants. Therefore, a systematic analysis and summary on the mechanisms of EPS as flame retardants is of significance for future application. On the basis of the advantages and disadvantages of other fire-resistant materials on the market, the characteristics and application potential of EPS are analyzed and summarized. Second, the possible fire-resistant mechanisms of phosphorus and alginate-like substance (ALE) in EPS are revealed, and the synergistic flame-retardant effects of extracellular-proteins are also elucidated. Based on this, the flame-retardant characteristics of EPS are comprehensively evaluated and compared with other fire-resistant materials. To further improving the performance of EPS as a flame-retardant material, some modification strategies are proposed, such as increasing their phosphorus content, purifying and enhancing the content of ALE in EPS, and optimizing the modification methods of EPS on their substrates.
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http://dx.doi.org/10.13227/j.hjkx.202010178DOI Listing
June 2021

Performance improvement in p-Type WSfield-effect transistors with 1T phase contacts.

Nanotechnology 2021 Jun 4;32(34). Epub 2021 Jun 4.

State Key Laboratory of ASIC and System, School of Microelectronics, Fudan University, Shanghai 200433, People's Republic of China.

The non-ideal contact between the metal electrode and semiconducting channel has become one of the major bottlenecks degrading the performance of field-effect transistors (FETs) based on two-dimensional (2D) materials. The formation of the Schottky barrier prohibiting the carrier injection as well as the Fermi level pinning effect have a strong impact on the device performance. In this work, we fabricated a 2D WSFET device with engineered metallic 1T phase at the source/drain region by using Li intercalation method. As compared to the device with conventional 2H-WSchannel, the engineered FET with a 2H-WS/1T-WSjunction has exhibited greatly improved performance such as over 10 times higher carrier mobility and steeper subthreshold slope. Such results have demonstrated the boosted carrier injection into the channel over the engineered metal contact as well as the reduced tunnel barrier width between the 2H/1T-WSjunction. This can be attractive for the large-scale integration of the 2D devices towards high-performance and high-reproducibility nanoelectronics applications.
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http://dx.doi.org/10.1088/1361-6528/ac037dDOI Listing
June 2021

Comparison of delta-shaped anastomosis and Billroth I reconstruction after laparoscopic distal gastrectomy for gastric cancer.

J Gastrointest Oncol 2021 Apr;12(2):259-267

Department of Gastrointestinal Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: This study intends to compare the short-term effects and long-term survival of gastric cancer patients who underwent delta-shaped anastomosis (DA) and Billroth I reconstructions after laparoscopic distal gastrectomy.

Methods: We retrospectively collected data from 257 patients with gastric cancer who underwent laparoscopic distal gastrectomy between January 2013 and December 2017. Patients were classified into 2 groups according to the reconstruction method used: the DA group (n=91) and the Billroth I group (n=166). The clinical data, short-term efficacy, and long-term results were compared between the 2 groups.

Results: The operation time (P<0.001) and the post-operative length of hospital stay (P<0.001) were shorter in the DA group than in the Billroth I group. The time to the first oral intake of a soft diet after surgery was earlier in the DA group than in the Billroth I group (P=0.014). Kaplan-Meier (log-rank test) analysis showed no significant difference in the 5-year survival rates between the 2 groups for patients at the same pathological stage. Multivariate analysis showed that abnormal carcinoembryonic antigen (CEA) (P=0.006), chemotherapy (P<0.001), T stage (P<0.001), and N stage (P<0.001) were independent prognostic factors for survival.

Conclusions: DA and Billroth I are feasible and safe reconstruction methods of the digestive tract after gastric cancer. DA is the recommended reconstruction method for laparoscopic distal gastrectomy.
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http://dx.doi.org/10.21037/jgo-21-109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107602PMC
April 2021

Management of Follow-Up With Preterm Infants During the Outbreak in China.

Front Pediatr 2021 29;9:637275. Epub 2021 Apr 29.

Department of Pediatrics, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.

Coronavirus disease 2019 (COVID-19) swept Wuhan in January 2020. Other cities in China also suffered during the pandemic. Routine medical services were conducted in the Neonatal Intensive Unit (NICU) as usual, but the follow-up after discharge was seriously affected. To investigate the feasibility and effectiveness of a combination of online and face-to-face follow-up for preterm infants during the COVID-19 epidemic and to explore a follow-up pattern that can provide follow-up services while maximizing the protection of preterm infants and soothing the fear of their parents. Preterm infants ( = 35) whose first follow-up appointment was scheduled from February 1 to April 30, 2020, and preterm infants ( = 43) in the NICU follow-up group who were discharged from January 1, 2018, to January 31, 2020, who had a second or later routine follow-up appointment scheduled from February 1 to April 30, 2020, were enrolled. We provided a combination of online and face-to-face follow-up for preterm infants surveyed with the Wenjuanxing platform before and after the online follow-up and compared the first-time follow-up rate between the outbreak and the same period of the previous year. Feeding and oral medicine and supplements were the most concerning problems of the parents of preterm infants. The anxiety level of the family was significantly decreased after online follow-up ( < 0.05). A total of 96.8% of parents were satisfied or very satisfied with online follow-up, and 95.2% of parents thought that online follow-up had answered all their questions. Only 35.5% of parents thought online follow-up could replace face-to-face follow-up. The combination of online and face-to-face follow-up alleviated the anxiety of the parents during the outbreak and achieved a similar first-time follow-up rate as the same period in 2019.
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http://dx.doi.org/10.3389/fped.2021.637275DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116626PMC
April 2021

Molecular Mechanism of Expression Changes of Immunological Indexes of PD-1/sPD-L1 after Radiotherapy in Nonsmall Cell Lung Cancer.

Biomed Res Int 2021 20;2021:8811751. Epub 2021 Apr 20.

Department of Radiation Oncology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang 110042, China.

It is aimed at investigating the changes of serum soluble programmed death-ligand 1 (sPD-L1) expression level in nonsmall cell lung cancer (NSCLC) before and after radiotherapy, the correlation of PD-L1, PD-1, and proteins of Akt (protein kinase B), mTOR, and HIF-1, and the molecular mechanism of the PD-1/PD-L1 pathway in the development of NSCLS. A total of 126 NSCLC patients receiving radiotherapy in Liaoning Cancer Hospital from September 2018 to September 2019 were selected as the observation group, and another 58 healthy volunteers were selected as the control group. NSCLC patients were divided into group A (stage I-II, stereotactic radiotherapy) and group B (stage III, intensity-modulated radiation therapy) according to the cancer stage. The efficacy of radiotherapy was evaluated, and sPD-L1 expression was detected by ELISA. The immunohistochemical staining was adopted to detect protein expressions of Akt, mTOR, and HIF-1 in NSCLC tissues. The correlation between their expression and expression of PD-L1 and PD-1 was analyzed. The results showed that the overall response rate (ORR) of group A was 89.29%, the clinical benefit response (CBR) was 96.43%, the median survival time (MST) was 25 months, and the survival rate within three years was 72.56%. In group B, the ORR was 70.41%, the CBR was 97.96%, the MST was 18 months, and the survival rate within three years was 34.67%. Comparison of overall serum sPD-L1 expression in the control group, group A, and group B and between groups before radiotherapy was statistically significant ( < 0.01). After radiotherapy, serum sPD-L1 expression in group A and group B decreased compared with that before radiotherapy ( < 0.01). Among NSCLC patients, the positive expression rate of Akt, mTOR, and HIF-1 was 71.32%, 41.26%, and 80.65%, respectively. PD-L1 expression and Akt, mTOR, and HIF-1 expression showed a significant correlation. PD1 expression and Akt, mTOR, and HIF-1 expression also showed a significant correlation. It indicated that the expression level of sPD-L1 in NSCLC patients was higher than that in normal subjects, but the expression level of sPD-L1 was decreased after radiotherapy. PD-1/PD-L1 may play important roles in NSCLC procession through the Akt/mTOR and HIF-1 pathway.
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http://dx.doi.org/10.1155/2021/8811751DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079205PMC
May 2021

Effect of the gradual increase of NaSO on performance and microbial diversity of aerobic granular sludge.

J Environ Manage 2021 Aug 10;292:112696. Epub 2021 May 10.

Department of Civil and Environmental Engineering, Harbin Institute of Technology, Shenzhen, Shenzhen, 518055, China.

Aerobic granular sludge (AGS) is a promising technology in treating saline wastewater. The effects of sodium sulfate on contaminant removal performance and sludge characteristics of AGS were studied. The results showed that under the stress of sodium sulfate, AGS kept good removal performance of ammonia nitrogen (NH+ 4-N), chemical oxygen demand (COD), and total nitrogen (TN), with removal efficiency reaching 98.7%, 91.5% and 62.7%, respectively. When sodium sulfate reached 14700 mg/L, nitrite oxidizing bacteria (NOB) were inhibited and nitrite accumulation occurred, but it had little impact on total phosphorus (TP) removal. Under the stress of sodium sulfate, compactness and settling performance of AGS was enhanced. The microbial community greatly varied and the microbial diversity of aerobic granular sludge has decreased under the stress of sodium sulfate. The study reveals that AGS has great potential in application on treating saline wastewater.
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http://dx.doi.org/10.1016/j.jenvman.2021.112696DOI Listing
August 2021

Whole-Genome Sequence of Synthesized Allopolyploids in Reveals Insights into the Genome Evolution of Allopolyploidization.

Adv Sci (Weinh) 2021 05 15;8(9):2004222. Epub 2021 Feb 15.

National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China.

The importance of allopolyploidy in plant evolution has been widely recognized. The genetic changes triggered by allopolyploidy, however, are not yet fully understood due to inconsistent phenomena reported across diverse species. The construction of synthetic polyploids offers a controlled approach to systematically reveal genomic changes that occur during the process of polyploidy. This study reports the first fully sequenced synthetic allopolyploid constructed from a cross between and , with high-quality assembly. The two subgenomes are confidently partitioned and the -originated subgenome predominates over the -originated subgenome, retaining more sequences and showing higher homeologous gene expression. Most of the genomic changes emerge immediately after interspecific hybridization. Analysis of a series of genome sequences from several generations (S, S-S) of . × confirms that genomic changes occurred in the very first generations, subsequently slowing down as the process of diploidization is initiated. The duplicated genome of the allopolyploid with double genes from both parents broadens the genetic base of . ×, resulting in enhanced phenotypic plasticity. This study provides novel insights into plant polyploid genome evolution and demonstrates a promising strategy for the development of a wide array of novel plant species and varieties through artificial polyploidization.
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http://dx.doi.org/10.1002/advs.202004222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097326PMC
May 2021

Arg-Vasotocin Directly Activates Isotocin Receptors and Induces COX2 Expression in Ovoviviparous Guppies.

Front Endocrinol (Lausanne) 2021 23;12:617580. Epub 2021 Apr 23.

Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, China.

Oxytocin (OT) is a crucial regulator of reproductive behaviors, including parturition in mammals. Arg-vasopressin (AVP) is a nonapeptide homologous to Arg-vasotocin (AVT) in teleosts that has comparable affinity for the OT receptor. In the present study, ovoviviparous guppies () were used to study the effect of AVT on delivery mediated by the activation of prostaglandin (PG) biosynthesis isotocin (IT) receptors (ITRs). One copy each of and and two copies of were identified in guppies. The results of the affinity assay showed that various concentrations of AVT and IT (10, 10, and 10 mol/L) significantly activated (P < 0.05). experiments revealed significant upregulation (P < 0.05) of cyclooxygenase 2 (), which is the rate-limiting enzyme involved in PG biosynthesis, and by AVT and IT. Furthermore, dual hybridization detected positive signals for and at the same site, implying that ITR1 may regulate gene expression. Measurement of prostaglandin F (PGF) concentrations showed that AVT induced PGF synthesis (P < 0.05) and that the effect of IT was not significant. Finally, intraperitoneal administration of PGF significantly induced premature parturition of guppies. This study is the first to identify and characterize AVT and ITRs in guppies. The findings suggest that AVT promotes PG biosynthesis ITR and that PGF induces delivery behavior in ovoviviparous guppies.
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http://dx.doi.org/10.3389/fendo.2021.617580DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104081PMC
April 2021

[Endoscopic Hemostasis and Its Related Factors of Duodenal Hemorrhage].

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2021 Apr;43(2):222-229

Department of Gastroenterology,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.

Objective To analyze clinical characteristics and short-term efficacy of endoscopic hemostasis in acute duodenal hemorrhage. Methods A retrospective study was conducted for the patients who received endoscopy in the PUMC Hospital due to upper gastrointestinal bleeding and were confirmed to be on account of duodenal lesions for bleeding from January 2011 to December 2018.Clinical information of patients was collected,including demographics,comorbidities,and medication use.Endoscopic information included the origin of bleeding,the number and location of lesions,Forrest classes and size of ulcers,and endoscopic therapeutic methods.Factors that could be relative to the failure of endoscopic hemostasis or short-term recurrence of hemorrhage in these patients were analyzed. Results Among all the patients with duodenal hemorrhage,79.7%(102/128)were due to ulcers,14.1%(18/128)to tumors,3.9%(5/128)to vascular malformation,and 2.3%(3/128)to diverticulum.Fifty-three(41.4%)patients received endoscopic hemostasis,and six patients(4.7%)received surgery or interventional embolization after the endoscopic test.Among the patients receiving endoscopic hemostasis,5.7%(3/53),66.0%(35/53),and 28.3%(15/53)received injection therapy,mechanical therapy,and dual endoscopic therapy,respectively,and 94.3% of them were cured.However,10(18.9%)of them experienced recurrence of hemorrhage and 3 patients died during hospitalization.Only one patient suffered from perforation after the second endoscopic treatment.Lesions located on the posterior wall of bulb appeared to be a risk factor for the failure of endoscopic hemostasis(OR=31.333,95% CI=2.172-452.072,P=0.021).The lesion diameter≥1 cm was a risk factor of rebleeding after endoscopic therapy(OR=7.000,95% CI=1.381-35.478,P=0.023).Conclusions Peptic ulcers were always blamed and diverticulum could also be a common reason for duodenal hemorrhage,which was different from the etiological constitution of acute upper gastrointestinal hemorrhage.Lesions locating on the posterior wall of the duodenum had a higher potential to fail the endoscopic hemostasis.The lesion diameter≥1 cm was a predictive factor for short-term recurrence.Forrest classes of ulcers at duodenum did not significantly affect the endoscopic therapeutic efficacy or prognosis.
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http://dx.doi.org/10.3881/j.issn.1000-503X.13045DOI Listing
April 2021

Enhanced proteins and amino acids production based on ammonia nitrogen assimilation and sludge increment by the integration of bioadsorption with anaerobic-anoxic-oxic (AAO) process.

Chemosphere 2021 Apr 30;280:130721. Epub 2021 Apr 30.

Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environment and Civil Engineering, Jiangnan University, Wuxi, 214122, China; Jiangsu College of Water Treatment Technology and Material Collaborative Innovation Center, Suzhou, 215009, China. Electronic address:

Poor effect of contaminants removal efficiency and low organic matter content of activated sludge are common in wastewater treatment plants (WWTPs) in China due to the low-strength wastewater. An anaerobic-anoxic-oxic (AAO) and an adsorption/AAO (A/AAO) combined system were established simultaneously to conduct a comparative study for realizing the conversion of carbon source in influent and the enrichment and recovery of proteins and amino acids through the assimilation of ammonia nitrogen. The experimental results showed that 63.5% of the organic matter in influent was adsorbed and flocculated in adsorption process, and the removal rates of chemical oxygen demand, total nitrogen and total phosphorus in A/AAO process were 88.7%, 77.1%, and 93.0% respectively, which were remarkably better than those in AAO process owing to the addition of improved carbon source. Ammonia assimilation rate of A/AAO process was 26.7% higher than that of AAO process, which implied that the ammonia used to synthesize sludge protein was prominently increased. Furthermore, intracellular proteins and amino acids in A/AAO process were 20% higher than those of AAO process, and the quality was equivalent with fish meal or soybean meal as feed. In addition, the microbial community analysis based on 16S rDNA was conducted. Dechloromonas, Zoogloea, Nitrospira, and Flavobacterium were the main genera, and played important roles in nutrient removal and ammonia nitrogen assimilation. The integration of adsorption process was significant to low-strength wastewater treatment and the improvement of excess sludge quality, which is a prospective inspiration for the resource recovery-based wastewater treatment process.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130721DOI Listing
April 2021

Selectively Metalizable Stereolithography Resin for Three-Dimensional DC and High-Frequency Electronics via Hybrid Additive Manufacturing.

ACS Appl Mater Interfaces 2021 May 7;13(19):22891-22901. Epub 2021 May 7.

State Key Laboratory of Millimetre Waves, School of Information Science and Engineering, Southeast University, Nanjing 210096, P. R. China.

Direct manufacturing of customized end-use electronic products is becoming an emerging trend of additive manufacturing (AM). This highly demands the evolution of the conventional AM processes from simply building single-material parts to simultaneously delivering complex structures and end-use functionalities. In this work, we propose a novel hybrid additive manufacturing solution that combines stereolithography (SLA) three-dimensional (3D) printing and laser-activated electroless plating for the manufacture of 3D fully functional electronic products. With our newly developed functional SLA resin that can be 3D printed, laser-activated, and thereafter selectively metalized, high-resolution circuitry can be free-formly patterned on 3D structures. In virtue of high-performance electrical materials, this technology is capable of creating not only 3D direct-current (DC) electronics but also 3D high-frequency devices like microwave/millimeter-wave antennas, which cannot be fabricated via traditional printed circuit board (PCB) technology and not even by most AM processes. This study represents a significant advance in additive manufacturing technologies, and more importantly offers a unique opportunity for the mass customization of fully functional 3D electronic products, which shows great potentials in consumer electronics, communication engineering, and automobile and aerospace industries.
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http://dx.doi.org/10.1021/acsami.1c01199DOI Listing
May 2021

Clinical Outcomes and Prognostic Factors in Patients With Recurrent Patellar Lateral Dislocation Treated With Isolated Medial Patellofemoral Ligament Reconstruction: A Retrospective Single-Center Analysis.

Orthop J Sports Med 2021 Apr 12;9(4):2325967121995803. Epub 2021 Apr 12.

Medical School of Chinese PLA, Beijing, China.

Background: Medial patellofemoral ligament reconstruction (MPFLR) is widely used to treat patellofemoral instability. However, it is still unclear when a concomitant bony procedure is needed.

Purpose: To evaluate the therapeutic efficacy of isolated MPFLR for recurrent patellar lateral dislocation and to identify the prognostic factors for clinical outcomes.

Study Design: Case series; Level of evidence, 4.

Methods: A total of 237 patients who had undergone unilateral isolated MPFLR between January 2011 and December 2016 were included. Patellar tracking (J sign) and radiologic information including patellar height, trochlear dysplasia, tibial tubercle-trochlear groove distance, and patellar tilt was collected preoperatively, and the Kujala and International Knee Documentation Committee (IKDC) functional scores were used to assess postoperative recovery. Failure was defined as redislocation or patient-reported and clinically confirmed patellar instability. The influence of radiologic features and the J sign on knee functional scores was explored via subgroup analysis. Potential prognostic factors were explored using univariate and multivariate regression analyses, and risk factors for a positive J sign were evaluated using Spearman coefficient correlation analysis.

Results: The mean ± SD follow-up period was 70.67 ± 18.05 months (range, 36-108 months). From preoperatively to final follow-up, all patients had statistically and clinically significant improvements in the Kujala score (from 51.43 ± 6.87 to 88.52 ± 4.83; < .001) and IKDC score (from 49.78 ± 6.05 to 81.16 ± 4.20; < .001). Subgroup analysis indicated no significant difference in functional score improvements based on radiologic features or the J sign. Overall, 20 knees (8.4%) experienced postoperative failure: 11 with redislocation and 9 with patellar instability. A preoperative positive J sign was shown to be an independent prognostic factor for postoperative failure via logistic regression analyses: univariate (odds ratio, 3.340; 95% CI, 1.316-8.480; = .011) and multivariate (odds ratio, 3.038; 95% CI, 1.179-7.827; = .021). In addition, the J sign was closely associated with patellar height (, 0.159; = .014) and trochlear dysplasia (, 0.235; < .001).

Conclusion: Isolated MPFLR appears to be an effective strategy for recurrent patellar lateral dislocation, with significant functional improvement and a low failure rate. A preoperative positive J sign was identified as a potential prognostic risk factor for postoperative failure.
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http://dx.doi.org/10.1177/2325967121995803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044575PMC
April 2021

Health risks from groundwater arsenic on residents in northern China coal-rich region.

Sci Total Environ 2021 Jun 2;773:145003. Epub 2021 Feb 2.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address:

Shanxi Province of northern China is a typical mining concentration and arsenism area. Years of mining activities have resulted in serious regional groundwater problems in Shanxi. Therefore, it is of great significance to know the health risk of groundwater arsenic on residents under the background of mining activities. Kriging interpolation was used to illustrate the spatio-temporal dynamics of the health risks on groundwater arsenic based on a ten-year investigation. The groundwater arsenic concentrations decreased over time and the distribution of high arsenic concentrations shrank. High arsenic concentrations were mainly distributed in the northern and middle basin areas. The forecasted area of high risks in coal mining areas was 5623 km, which was larger than that in non-coal mining areas. The residents living around mining areas were more vulnerable to exposure to groundwater arsenic. Further, the output map outlines the high-risk zones in order to protect the safety of drinking water for residents. This study may be helpful for the policy-makers to adopt a lower limit for groundwater arsenic to the worst affected regions and groups.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145003DOI Listing
June 2021

Erratum: Chlorido-tetra-kis-(imidazole)-copper(II) chloride. Corrigendum.

Acta Crystallogr E Crystallogr Commun 2021 Apr 31;77(Pt 4):450-451. Epub 2021 Mar 31.

Department of Materials Science and Technology, Taishan University, Taian 271021, People's Republic of China.

[This corrects the article DOI: 10.1107/S1600536807044108.].
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http://dx.doi.org/10.1107/S2056989021002267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025866PMC
April 2021

Orchestrated cellular, biochemical, and biomechanical optimizations endow platelet-rich plasma-based engineered cartilage with structural and biomechanical recovery.

Bioact Mater 2021 Nov 10;6(11):3824-3838. Epub 2021 Apr 10.

Department of Orthopedics, Chinese PLA General Hospital, Haidian, Beijing, 100853, China.

Recently, biomaterials for cartilage regeneration has been intensively investigated. However, the development of scaffolds that capture regenerated cartilage with biomechanical and structural recovery has rarely been reported. To address this challenge, platelet-rich plasma (PRP)-based cartilage constructs with a well-orchestrated symphony of cellular, biochemical and biomechanical elements were prepared by simultaneously employing chondrogenic progenitor cells (CPCs) as a cell source, optimizing platelet concentration, and adding an enzyme-ion activator. It was shown that this triple-optimized PRP + CPC construct possessed increased biomechanical properties and suitable biochemical signals. The following study demonstrated that the triple-optimized PRP + CPC constructs generated cartilage-like tissue with higher expression levels of chondrogenic-specific markers, more deposition of cartilage-specific extracellular matrix (ECM), and greater biomechanical values than those of the other constructs. Twelve weeks after the construct was implanted in a cartilage defect , histological analysis, qPCR, and biomechanical tests collectively showed that the triple-optimized constructs yielded a more chondrocyte-like cell phenotype with a higher synthesis of Col-II and aggrecan. More importantly, the triple-optimized constructs facilitated cartilage regeneration with better biomechanical recovery than that of the other constructs. These results demonstrate the efficacy of the triple-optimization strategy and highlight the simplicity and potency of this PRP + CPC construct for cartilage regeneration.
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http://dx.doi.org/10.1016/j.bioactmat.2021.03.037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065202PMC
November 2021

Efficacy and safety of non-surgical short-wave radiofrequency treatment of mild-to-moderate erythematotelangiectatic rosacea: a prospective, open-label pilot study.

Arch Dermatol Res 2021 May 2. Epub 2021 May 2.

Department of Dermatology, Xiangya Hospital, Central South University, Xiangya Road, Kaifu District, Changsha, 410008, Hunan, China.

Although various treatments have been proposed for the management of rosacea, achieving complete remission of persistent erythema remains challenging. Short-wave radiofrequency (SWRF) treatment has been shown to repair skin barriers and reduce chronic inflammation. However, limited studies have evaluated the effectiveness of SWRF treatment for erythematotelangiectatic rosacea (ETR). A prospective, open-label pilot study using SWRF therapy was conducted on 30 patients with mild-to-moderate ETR. During the first stage, the patients underwent a single, full-face treatment and were evaluated before and after the session, as well as on the 7th and 15th day post-treatment. During the second stage, ten treatment sessions were administered, and the patients were evaluated before and after the tenth session, as well as 1 month after the treatment. Adverse events were recorded during each treatment session, and the patients were followed up for 3 months after the last session. Twenty-eight patients completed the entire trial. On the 7th day after the single treatment, the global score (total score of flushing, persistent erythema, and telangiectasia) of ETR improved from 5.23 ± 1.09 to 4.00 ± 0.76 relative to the baseline value (p < 0.05); moreover, the overall treatment satisfaction improved from 7.27 ± 0.89 to 4.90 ± 0.91 (p < 0.05). 1 month after the tenth treatment session, the global score improved from 5.30 ± 1.01 to 3.85 ± 0.93 (p < 0.05), and the overall treatment satisfaction improved from 7.13 ± 0.85 to 5.17 ± 1.19 (p < 0.05). During the 3 month follow-up period, there were two cases of recurrence. Therefore, this report indicates that SWRF might be an effective auxiliary treatment for mild-to-moderate ETR.
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http://dx.doi.org/10.1007/s00403-021-02225-yDOI Listing
May 2021

MicroRNA-29c Acting on FOS Plays a Significant Role in Nonalcoholic Steatohepatitis Through the Interleukin-17 Signaling Pathway.

Front Physiol 2021 1;12:597449. Epub 2021 Apr 1.

Department of Infectious Disease, The First Affiliated Hospital of Wenzhou Medical University, Zhejiang Provincial Key Laboratory for Accurate Diagnosis and Treatment of Chronic Liver Disease, Hepatology Institute of Wenzhou Medical University, Wenzhou, China.

Nonalcoholic fatty liver disease is the most common hepatic disease in western countries and is even more ubiquitous in Asian countries. Our study determined that TH17/Treg cells were imbalanced in animal models. Based on our interest in the mechanism underlying TH17/Treg cell imbalance in nonalcoholic fatty liver mice, we conducted a joint bioinformatics analysis to further investigate this process. Common gene sequencing analysis was based on one trial from one sequencing platform, where gene expression analysis and enrichment analysis were the only analyses performed. We compared different sequencing results from different trials performed using different sequencing platforms, and we utilized the intersection of these analytical results to perform joint analysis. We used a bioinformatics analysis method to perform enrichment analysis and map interaction network analysis and predict potential microRNA sites. Animal experiments were also designed to validate the results of the data analysis based on quantitative polymerase chain reaction (qPCR) and western blotting. Our results revealed 8 coexisting differentially expressed genes (DEGs) and 7 hinge genes. The identified DEGs may influence nonalcoholic steatosis hepatitis through the interleukin-17 pathway. We found that microRNA-29c interacts with FOS and IGFBP1. Polymerase chain reaction analyses revealed both FOS and microRNA-29c expression in NASH mice, and western blot analyses indicated the same trend with regard to FOS protein levels. Based on these results, we suggest that microRNA-29c acts on FOS via the interleukin-17 signaling pathway to regulate TH17/Treg cells in NASH patients.
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http://dx.doi.org/10.3389/fphys.2021.597449DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078210PMC
April 2021