Publications by authors named "Jeung-Hoon Lee"

188 Publications

Clinical outcomes in adult patients with plaque psoriasis treated with ustekinumab under real-world practice in Korea: A prospective, observational, multi-center, postmarketing surveillance study.

J Dermatol 2021 Feb 2. Epub 2021 Feb 2.

Department of Dermatology, Chonnam National University Hospital, Gwangju, Korea.

Postmarketing surveillance is conducted to establish drug safety and effectiveness under real-world practice. We aimed to validate the effectiveness and safety of ustekinumab in the treatment of adult Korean patients with plaque psoriasis under real-world practice. This was a prospective, observational, and multi-center study. Subjects aged 18 years or older who were treated with ustekinumab for plaque psoriasis were enrolled. We enrolled 977 patients; 654 (66.9%) were men, with mean body surface area (BSA, ± standard deviation) of 27.0 ± 18.3% and mean psoriasis area severity index (PASI) score of 18.1 ± 9.7. The effectiveness analysis was performed in 581 patients who had at least one follow-up assessment and met treatment criteria per local label and reimbursement guidelines. Of these patients, 287 had effectiveness data for visit 6 at 53.7 ± 2.1 weeks. At visit 6, 91.6% (263/287), 51.2% (147/287), and 9.4% (27/287) patients achieved PASI 75, 90, and 100 responses, respectively. Adverse events (AEs) occurred in 112 of the 977 (11.5%) patients with an incidence rate of 21.5 per 100 patient-years (PYs). Serious AEs occurred in eight (0.8%) patients with an incidence rate of 1.2 per 100 PYs. The estimated 1-year drug survival rate was 87.7%. The multiple logistic regression analysis showed that higher baseline PASI score and no prior biologic exposure were significant predictors for PASI 90 response at visit 6. Ustekinumab was effective and safe, and displayed a high survival rate in the treatment of adult Korean patients with plaque psoriasis in real-world practice.
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http://dx.doi.org/10.1111/1346-8138.15670DOI Listing
February 2021

Estimation of the Complex Dynamic Stiffness of Inflated Rubber Diaphragms in Pneumatic Springs Using Finite Element Method.

Sensors (Basel) 2020 Nov 25;20(23). Epub 2020 Nov 25.

School of Mechanical Engineering, Changwon National University, Uichang-gu, Changwon 51140, Korea.

The accurate modeling of the complex dynamic stiffness of inflated rubber diaphragms in pneumatic springs is necessary for an efficient design of vibration isolation tables for precision instruments, such as optical devices and nano-scale equipment. In addition to pressurized air, rubber diaphragms, essentially employed for the prevention of air leakage, make a significant contribution to the total complex stiffness. To reflect the effect of the dynamic stiffness of the inflated rubber diaphragm on the total complex stiffness during the initial design or design improvement stage, it is desirable to predict the complex stiffness of the inflated rubber diaphragm beforehand. In this paper, an estimation method for the complex stiffness of inflated rubber diaphragms using the commercial finite element method (e.g., ABAQUS) is proposed. The proposed method reflects their dynamic characteristics under the large static deformation by the Mooney-Rivlin and Morman's constitutive equations. The results of comparison with experimental results indicate that the predictions obtained by the proposed method are congruent with the experimental values of the diaphragm.
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http://dx.doi.org/10.3390/s20236728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7728140PMC
November 2020

Exome sequencing reveals novel candidate gene variants associated with clinical characteristics in alopecia areata patients.

J Dermatol Sci 2020 Sep 14;99(3):216-220. Epub 2020 Aug 14.

Department of Dermatology, Chungnam National University, Daejeon, South Korea. Electronic address:

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http://dx.doi.org/10.1016/j.jdermsci.2020.08.003DOI Listing
September 2020

Corrigendum to "Stem Cell Membrane-Coated Au-Ag-PDA Nanoparticle-Guided Photothermal Acne Therapy", Colloids and Surfaces B: Biointerfaces (2020) 192C/COLSUB_111145].

Colloids Surf B Biointerfaces 2020 Dec 11;196:111314. Epub 2020 Aug 11.

Department of Dermatology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.

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http://dx.doi.org/10.1016/j.colsurfb.2020.111314DOI Listing
December 2020

Stem Cell Membrane-Coated Au-Ag-PDA Nanoparticle-Guided Photothermal Acne Therapy.

Colloids Surf B Biointerfaces 2020 May 20;192:111145. Epub 2020 May 20.

Department of Dermatology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China. Electronic address:

The polydopamine coating on Au-Ag nanoparticles (Au-Ag-PDA) possess excellent photothermal conversion efficiency after absorbing near-infrared laser light. After the stem cell membrane (STCM) encapsulates Au-Ag-PDA (Au-Ag-PDA@STCM), the nanoparticles (NPs) exhibit less cytotoxicity, and further optimizing their efficiency in photothermal therapy. The photothermal activity of Au-Ag-PDA@STCM has not yet been reported. Therefore, in this study, the sebaceous gland cell line SZ95 and the golden hamsters were used to observe the photothermal effects of the Au-Ag-PDA@STCM. SZ95 cells were treated with various concen-trations of Au-Ag-PDA@STCM NPs. The photothermal effect on cell proliferation was analyzed after irradiating the cells with a 808 nm laser. After laser treatment of golden hamsters, the flank organs were observed at 4 different time points. Histological analysis was performed to observe tissue damage. The results suggest that Au-Ag-PDA@STCM NPs significantly inhibited the proliferation of sebaceous gland cells in vitro, and reduced the size of sebaceous glands and sebum secretion in vivo. Therefore, NPs can be used to treat acne by thermally injuring sebaceous gland cells.
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http://dx.doi.org/10.1016/j.colsurfb.2020.111145DOI Listing
May 2020

Efficacy and safety of guselkumab compared with placebo and adalimumab in Korean patients with moderate-to-severe psoriasis: analysis from the phase III, double-blind, placebo- and active-comparator-controlled VOYAGE 1/2 trials.

J Dermatolog Treat 2020 May 27:1-7. Epub 2020 May 27.

Medical Affairs, Janssen Korea Ltd., Seoul, Korea.

The phase 3 studies, VOYAGE 1 and 2, were conducted to assess guselkumab in the treatment of patients with moderate-to-severe psoriasis. To investigate the efficacy and safety of guselkumab in Korean patients. The Korean sub-population of VOYAGE 1 and 2 study patients were included in this analysis. Efficacy and safety were evaluated through Weeks 24 and 28, respectively. Of 126 randomized Korean patients, 30, 63, and 33 received placebo, guselkumab, and adalimumab, respectively. At Week 16, guselkumab was superior to placebo in achieving an Investigator's Global Assessment (IGA) score of 0 or 1 (cleared or minimal; 90.5 vs. 20.0%, <.001) and a Psoriasis Area and Severity Index (PASI) 90 response (71.4 vs. 3.3%, <.001). At week 24, a significantly higher proportion of guselkumab-treated patients achieved PASI 75 and IGA 0 (clear skin) responses compared to adalimumab-treated patients (PASI 75: 93.7 vs. 66.7%, <.001; IGA 0: 52.4 vs. 21.2%, =.004). Through Week 28, guselkumab and adalimumab showed comparable safety profiles. The efficacy and safety of guselkumab in Korean psoriasis patients through 28 weeks were consistent with findings for the overall VOYAGE 1 and 2 study population.
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http://dx.doi.org/10.1080/09546634.2020.1770174DOI Listing
May 2020

Deficiency of Crif1 in hair follicle stem cells retards hair growth cycle in adult mice.

PLoS One 2020 24;15(4):e0232206. Epub 2020 Apr 24.

Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Korea.

Hair growth is the cyclically regulated process that is characterized by growing phase (anagen), regression phase (catagen) and resting phase (telogen). Hair follicle stem cells (HFSCs) play pivotal role in the control of hair growth cycle. It has been notified that stem cells have the distinguished metabolic signature compared to differentiated cells, such as the preference to glycolysis rather than mitochondrial respiration. Crif1 is a mitochondrial protein that regulates the synthesis and insertion of oxidative phosphorylation (OXPHOS) polypeptides to inner membrane of mitochondria. Several studies demonstrate that tissue-specific knockout of Crif1 leads to mitochondrial dysfunction. In this study, we investigated the effect of mitochondrial dysfunction in terms of Crif1 deficiency on the hair growth cycle of adult mice. We created two kinds of inducible conditional knockout (icKO) mice. In epidermal specific icKO mice (Crif1 K14icKO), hair growth cycle was significantly retarded compared to wild type mice. Similarly, HFSC specific icKO mice (Crif1 K15icKO) showed significant retardation of hair growth cycle in depilation-induced anagen model. Interestingly, flow cytometry revealed that HFSC populations were maintained in Crif1 K15icKO mice. These results suggest that mitochondrial function in HFSCs is important for the progression of hair growth cycle, but not for maintenance of HFSCs.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0232206PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182249PMC
July 2020

Tumor Suppressive Function of NQO1 in Cutaneous Squamous Cell Carcinoma (SCC) Cells.

Biomed Res Int 2019 22;2019:2076579. Epub 2019 Nov 22.

Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.

Cutaneous squamous cell carcinoma (SCC) is a common cancer that significantly decreases the quality of life. It is known that external stimulus such as ultraviolet (UV) radiation induces cutaneous SCC via provoking oxidative stress. NAD(P)H dehydrogenase 1 (NQO1) is a ubiquitous flavoenzyme that functions as a guardian against oxidative stress. However, the effect of NQO1 on cutaneous SCC is not clearly elucidated. In this study, we investigated the effect of NQO1 on cutaneous SCC cells using the recombinant adenoviruses that can upregulate and/or downregulate NQO1 expression. Overexpression of NQO1 resulted in significant decrease of cell proliferation and colony forming activity of SCC lines (SCC12 and SCC13 cells). By contrast, knockdown of NQO1 increased the cell proliferation and colony forming activity. Accordingly, the levels of proliferation-related regulators, such as Cyclin D1, Cyclin E, PCNA, SOX2, and p63, were decreased by the overexpression of NQO1, while those were increased by knockdown of NQO1. In addition, NQO1 affected the invasion and migration of SCC cells in a very similar way, with the regulation of epithelial-mesenchymal transition- (EMT-) related molecules, including E-cadherin, N-cadherin, Vimentin, Snail, and Slug. Finally, the overexpression of NQO1 decreased the level of phosphorylated AKT, JNK, and p38 MAPK, while the knockdown of NQO1 increased the level of phosphorylated signaling molecules. Based on these data, NQO1 has tumor suppressive function in cutaneous SCC cells.
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http://dx.doi.org/10.1155/2019/2076579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893255PMC
May 2020

KLF4 suppresses the tumor activity of cutaneous squamous cell carcinoma (SCC) cells via the regulation of SMAD signaling and SOX2 expression.

Biochem Biophys Res Commun 2019 09 6;516(4):1110-1115. Epub 2019 Jul 6.

Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, South Korea; Department of Medical Science, School of Medicine, Chungnam National University, Daejeon, South Korea. Electronic address:

Kruppel-like factor 4 (KLF4) is a zinc-finger transcription factor that plays a role in terminal differentiation of epidermal keratinocytes. There are conflicting reports regarding the role of KLF4 in tumor development, with both the tumor suppressive and/or oncogenic properties depending on different conditions and cell types. In this study, we investigated the functional importance of KLF4 in cutaneous squamous cell carcinoma (SCC). Immunohistochemistry showed that KLF4 expression was relatively low in SCC lesion compared to normal epidermis. To examine the effects of KFL4, we transduced SCC lines (SCC12 and SCC13 cells) with the KLF4-expressing recombinant adenovirus. Overexpression of KLF4 significantly decreased cell proliferation and colony forming activity. In addition, overexpression of KLF4 markedly reduced invasive potential, along with the downregulation of epithelial-mesenchymal transition (EMT)-related molecules. In a mechanistic study, KLF4 inhibited SOX2, of which expression is critical for tumor initiation and growth of SCC. Further investigations indicated that SOX2 expression is induced by TGF-β/SMAD signaling, and that overexpression of KLF4 inhibited SMAD signaling via upregulation of SMAD7, an important inhibitory SMAD molecule. Based on these data, KLF4 plays a tumor suppressive role in cutaneous SCC cells.
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http://dx.doi.org/10.1016/j.bbrc.2019.07.011DOI Listing
September 2019

Antitumor Effect of Albendazole on Cutaneous Squamous Cell Carcinoma (SCC) Cells.

Biomed Res Int 2019 9;2019:3689517. Epub 2019 Jun 9.

Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Republic of Korea.

Drug repurposing and/or repositioning is an alternative method to develop new treatment for certain diseases. Albendazole was originally developed as an anthelmintic medication, and it has been used to treat a variety of parasitic infestations. In this study, we investigated the antitumor effect of albendazole and putative action mechanism. Results showed that albendazole dramatically decreased the cell viability of SCC cell lines (SCC12 and SCC13 cells). Albendazole increased apoptosis-related signals, including cleaved caspase-3 and PARP-1 in a dose-dependent fashion. The mechanistic study showed that albendazole induced endoplasmic reticulum (ER) stress, evidenced by increase of CHOP, ATF-4, caspase-4, and caspase-12. Pretreatment with ER stress inhibitor 4-PBA attenuated albendazole-induced apoptosis of SCC cells. In addition, albendazole decreased the colony-forming ability of SCC cells, together with inhibition of Wnt/-catenin signaling. These results indicate that albendazole shows an antitumor effect via regulation of ER stress and cancer stemness, suggesting that albendazole could be repositioned for cutaneous SCC treatment.
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http://dx.doi.org/10.1155/2019/3689517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590486PMC
December 2019

Tropomyosin-receptor kinase fused gene (TFG) regulates lipid production in human sebocytes.

Sci Rep 2019 04 29;9(1):6587. Epub 2019 Apr 29.

Department of Medical Science, School of Medicine, Chungnam National University, Daejeon, Korea.

The endoplasmic reticulum (ER) is an organelle in which important cellular events such as protein synthesis and lipid production occur. Although many lipid molecules are produced in the ER, the effect of ER-organizing proteins on lipid synthesis in sebocytes has not been completely elucidated. Tropomyosin-receptor kinase fused gene (TFG) is located in ER exit sites and participates in COPII-coated vesicle formation along with many scaffold proteins, such as Sec. 13 and Sec. 16. In this study, we investigated the putative role of TFG in lipid production in sebocytes using an immortalized human sebocyte line. During IGF-1-induced lipogenesis, the level of the TFG protein was increased in a time- and dose-dependent manner. When TFG was over-expressed using recombinant adenovirus, lipid production in sebocytes was increased along with an up-regulation of the expression of lipogenic regulators, such as PPAR-γ, SREBP-1 and SCD. Conversely, down-regulation of TFG using a microRNA (miR) decreased lipid production and the expression of lipogenic regulators. Based on these data, TFG is a novel regulator of lipid synthesis in sebocytes.
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http://dx.doi.org/10.1038/s41598-019-43209-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488642PMC
April 2019

Induction of alopecia areata in C3H/HeJ mice using polyinosinic-polycytidylic acid (poly[I:C]) and interferon-gamma.

Sci Rep 2018 08 21;8(1):12518. Epub 2018 Aug 21.

Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Korea.

Alopecia areata (AA) is a chronic, relapsing hair-loss disorder that is considered to be a T-cell-mediated autoimmune disease. Several animal models for AA have been created to investigate the pathophysiology and screen for effective therapeutic targets. As C3H/HeJ mice develop AA spontaneously in a low frequency, a novel animal model is needed to establish an AA-like condition faster and more conveniently. In this study, we present a novel non-invasive AA rodent model that avoids skin or lymph-node cell transfer. We simply injected C3H/HeJ mice subcutaneously with interferon-gamma (IFNγ) along with polyinosinic:polycytidylic acid (poly[I:C]), a synthetic dsRNA, to initiate innate immunity via inflammasome activation. Approximately 80% of the IFNγ and poly(I:C) co-injected mice showed patchy AA lesions after 8 weeks. None of the mice displayed hair loss in the IFNγ or poly(I:C) solely injection group. Immunohistochemical staining of the AA lesions revealed increased infiltration of CD4 and CD8 cells infiltration around the hair follicles. IFNγ and poly(I:C) increased the expression of NLRP3, IL-1β, CXCL9, CXCL10, and CXCL11 in mouse skin. Taken together, these findings indicate a shorter and more convenient means of AA animal model induction and demonstrate that inflammasome-activated innate immunity is important in AA pathogenesis.
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http://dx.doi.org/10.1038/s41598-018-30997-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6104095PMC
August 2018

Destructive frequency of oblate spheroidal air-balloon for suppression of propeller cavitation induced hull excitation.

J Acoust Soc Am 2018 Jul;144(1):186

System Dynamics Research, Korea Institute of Machinery and Materials, Yuseong-gu, Daejeon, 34103, South Korea.

The air-balloon can effectively neutralize hull excitations induced by the propeller cavitation. For the design, it is essential to derive the destructive frequency of an oblate spheroidal air-bubble, which is elaborated on in this paper. Beginning with the exact modal-series solution proposed by Yeh [Ann. Phys. 468, 53-61 (1964)], an approximated form of the scattered pressure is set up by assuming that the acoustic wavelength is much larger than the size of the balloon in the low frequency ranges. An algebraic formula for the destructive frequency can then be written as a function of the resonance frequency and a spatial variable. It is well known that the resonance frequency of a deformed bubble is higher than that of an ideal spherical one with the same volume. In addition to this, the current investigation puts an emphasis on the fact that asphericity induces a more severe shift of the destructive frequency than the resonance frequency, and that its effect needs to be reflected in the balloon design.
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http://dx.doi.org/10.1121/1.5044755DOI Listing
July 2018

Possible Role of Single Stranded DNA Binding Protein 3 on Skin Hydration by Regulating Epidermal Differentiation.

Ann Dermatol 2018 Aug 28;30(4):432-440. Epub 2018 Jun 28.

Department of Dermatology, College of Medicine, Chungnam National University, Daejeon, Korea.

Background: Skin hydration is a common problem both in elderly and young people as dry skin may cause irritation, dermatological disorders, and wrinkles. While both genetic and environmental factors seem to influence skin hydration, thorough genetic studies on skin hydration have not yet been conducted.

Objective: We used a genome-wide association study (GWAS) to explore the genetic elements underlying skin hydration by regulating epidermal differentiation and skin barrier function.

Methods: A GWAS was conducted to investigate the genetic factors influencing skin hydration in 100 Korean females along with molecular studies of genes in human epidermal keratinocytes for functional study in vitro.

Results: Among several single nucleotide polymorphisms identified in GWAS, we focused on Single Stranded DNA Binding Protein 3 (SSBP3) which is associated with DNA replication and DNA damage repair. To better understand the role of SSBP3 in skin cells, we introduced a calcium-induced differentiation keratinocyte culture system model and found that SSBP3 was upregulated in keratinocytes in a differentiation dependent manner. When SSBP3 was overexpressed using a recombinant adenovirus, the expression of differentiation-related genes such as loricrin and involucrin was markedly increased.

Conclusion: Taken together, our results suggest that genetic variants in the intronic region of could be determinants in skin hydration of Korean females. represents a new candidate gene to evaluate the molecular basis of the hydration ability in individuals.
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http://dx.doi.org/10.5021/ad.2018.30.4.432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6029969PMC
August 2018

Three Streams for the Mechanism of Hair Graying.

Ann Dermatol 2018 Aug 28;30(4):397-401. Epub 2018 Jun 28.

Department of Anatomy, Chungnam National University College of Medicine, Daejeon, Korea.

Hair graying is an obvious sign of human aging. Although graying has been investigated extensively, the mechanism remains unclear. Here, we reviewed previous studies on the mechanism of graying and seek to offer some new insights. The traditional view is that hair graying is caused by exhaustion of the pigmentary potential of the melanocytes of hair bulbs. Melanocyte dysfunction may be attributable to the effects of toxic reactive oxygen species on melanocyte nuclei and mitochondria. A recent study suggests that bulge melanocyte stem cells (MSCs) are the key cells in play. Graying may be caused by defective MSC self-maintenance, not by any deficiency in bulbar melanocytes. Our previous study suggested that graying may be principally attributable to active hair growth. Active hair growth may produce oxidative or genotoxic stress in hair bulge. These internal stress may cause eventually depletion of MSC in the hair follicles. Taken together, hair graying may be caused by MSC depletion by genotoxic stress in the hair bulge. Hair graying may also be sometimes caused by dysfunction of the melanocytes by oxidative stress in the hair bulb. In addition, hair graying may be attributable to MSC depletion by active hair growth.
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http://dx.doi.org/10.5021/ad.2018.30.4.397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6029974PMC
August 2018

Induction of pigmentation by a small molecule tyrosine kinase inhibitor nilotinib.

Biochem Biophys Res Commun 2018 09 28;503(4):2271-2276. Epub 2018 Jun 28.

Department of Dermatology and Institute of Health Sciences, School of Medicine, Gyeongsang National University & Hospital, Jinju, South Korea. Electronic address:

Skin color is determined by the melanin pigments that are produced in melanocytes then transferred to surrounding keratinocytes. Despite the growing number of commercial products claiming the pigmentation-regulatory effects, there is still a demand for the development of new materials that are safe and more efficacious. We tried to screen the pigmentation-regulatory materials using a commercially available drugs, and found that nilotinib could induce pigmentation in melanoma cells. When HM3KO melanoma cells were treated with nilotinib, melanin content was increased together with increase of tyrosinase activity. Nilotinib increased the expression of pigmentation-related genes such as MITF, tyrosinase and TRP1. Consistent with these results, the protein level for MITF, tyrosinase, and TRP1 was significantly increased by nilotinib. To delineate the action mechanism of nilotinib, we investigated the effects of nilotinib on intracellular signaling. As a result, nilotinib decreased the phosphorylation of AKT, while increased the phosphorylation of CREB. The pretreatment of PKA inhibitor H89 markedly blocked the nilotinib-induced phosphorylation of CREB. In accordance with, pretreatment of H89 significantly inhibited the nilotinib-induced pigmentation, indicating that nilotinib induces pigmentation via the activation of PKA signaling. Together, our data suggest that nilotinib can be developed for the treatment of hypopigmentary disorder such as vitiligo.
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http://dx.doi.org/10.1016/j.bbrc.2018.06.148DOI Listing
September 2018

Isoginkgetin Inhibits Insulin-Like Growth Factor-1-Induced Sebum Production in Cultured Human Sebocytes.

Ann Dermatol 2018 Jun 23;30(3):394-396. Epub 2018 Apr 23.

Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.

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http://dx.doi.org/10.5021/ad.2018.30.3.394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5929972PMC
June 2018

β-Catenin Regulates the Expression of cAMP Response Element-Binding Protein 1 in Squamous Cell Carcinoma Cells.

Ann Dermatol 2018 Feb 26;30(1):119-122. Epub 2017 Dec 26.

Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.

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http://dx.doi.org/10.5021/ad.2018.30.1.119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762468PMC
February 2018

Sea-trial verification of ultrasonic antifouling control.

Biofouling 2018 01 12;34(1):98-110. Epub 2017 Dec 12.

b School of Mechanical Engineering , Changwon National University , Changwon, Gyeongnam , Republic of Korea.

An ultrasonic antifouling treatment was applied to a 96,000 m class drill-ship to verify its feasibility through a sea-trial. Soon after the hull cleaning had been performed, six ultrasonic projectors were evenly deployed around the starboard shell plate. Driven by a 23 kHz sinusoidal ultrasound in an intermittent manner, the projectors emitted a high-intensity sound reaching 214 dB at the source level causing cavitation around the adjacent water and eventually deterring the settlement of marine fouling organisms. Underwater photographs acquired after four months showed fairly clean slabs on the starboard side, but heavy fouling on the port side. This experiment revealed that ultrasound treatment is a promising method for inhibiting fouling accumulation, even for large-scale ship applications.
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http://dx.doi.org/10.1080/08927014.2017.1409347DOI Listing
January 2018

Corrigendum: The Effect of Micro-Spicule Containing Epidermal Growth Factor on Periocular Wrinkles.

Ann Dermatol 2017 Dec 30;29(6):828. Epub 2017 Oct 30.

Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.

[This corrects the article on p. 187 in vol. 29, PMID: 28392646.].
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http://dx.doi.org/10.5021/ad.2017.29.6.828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705381PMC
December 2017

Incidental Focal Acantholytic Dyskeratosis in a Patient with Discoid Lupus Erythematosus: A Possible Role for SPCA1 in the Pathogenesis of the Disease.

Ann Dermatol 2017 Oct 25;29(5):655-657. Epub 2017 Aug 25.

Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.

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http://dx.doi.org/10.5021/ad.2017.29.5.655DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597671PMC
October 2017

The Effect of FK 506 on the Reepithelialization of Superficial Skin Wound.

Ann Dermatol 2017 Oct 25;29(5):635-637. Epub 2017 Aug 25.

Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.

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http://dx.doi.org/10.5021/ad.2017.29.5.635DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597662PMC
October 2017

Possible role of tropomyosin-receptor kinase fused gene on skin collagen remodeling.

J Dermatol Sci 2017 Dec 24;88(3):375-377. Epub 2017 Aug 24.

Department of Dermatology, College of Medicine, Chungnam National University, Daejeon, Republic of Korea; Department of Biochemistry and Molecular Biology, School of Medicine, KyungHee University, Seoul, Republic of Korea; LG Household and Healthcare, Daejeon, Republic of Korea; Department of Dermatology, College of Medicine, Chungnam National University, Daejeon, Republic of Korea. Electronic address:

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http://dx.doi.org/10.1016/j.jdermsci.2017.08.010DOI Listing
December 2017

Scar Sarcoidosis Developed after Blepharoplasty in Acute Lymphoblastic Leukemia Patient.

Ann Dermatol 2017 Aug 21;29(4):511-513. Epub 2017 Jun 21.

Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.

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http://dx.doi.org/10.5021/ad.2017.29.4.511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5500727PMC
August 2017

Inhibitory effect of 5-iodotubercidin on pigmentation.

Biochem Biophys Res Commun 2017 09 4;490(4):1282-1286. Epub 2017 Jul 4.

Department of Dermatology and Institute of Health Sciences, School of Medicine, Gyeongsang National University & Hospital, Jinju, Republic of Korea. Electronic address:

Melanin pigments are the primary contributors for the skin color. They are produced in melanocytes and then transferred to keratinocytes, eventually giving various colors on skin surface. Although many depigmenting and/or skin-lightening agents have been developed, there is still a growing demand on materials for reducing pigmentation. We attempted to find materials for depigmentation and/or skin-lightening using the small molecule compounds commercially available, and found that 5-iodotubercidin had inhibitory potential on pigmentation. When HM3KO melanoma cells were treated with 5-iodotubercidin, pigmentation was dramatically reduced. The 5-iodotubercidin decreased the protein level for pigmentation-related molecules such as MITF, tyrosinase, and TRP1. In addition, 5-iodotubercidin decreased the phosphorylation of CREB, while increased the phosphorylation of AKT and ERK. These data suggest that 5-iodotubercidin inhibits melanogenesis via the regulation of intracellular signaling related with pigmentation. Finally, 5-iodotubercidin markedly inhibited the melanogenesis of zebrafish embryos, an in vivo evaluation model for pigmentation. Together, these data suggest that 5-iodotubercidin can be developed as a depigmenting and/or skin-lightening agent.
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http://dx.doi.org/10.1016/j.bbrc.2017.07.008DOI Listing
September 2017

Effects of Brn2 overexpression on eccrine sweat gland development in the mouse paw.

Biochem Biophys Res Commun 2017 08 23;490(3):901-905. Epub 2017 Jun 23.

Department of Anatomy, College of Medicine, Chungnam National University, Daejeon, South Korea. Electronic address:

Eccrine sweat glands regulate body temperature by secreting water and electrolytes. In humans, eccrine sweat glands are ubiquitous in the skin, except in the lips and external genitalia. In mice, eccrine sweat glands are present only in the paw pad. Brn2 is a protein belonging to a large family of transcription factors. A few studies have examined Brn2 in melanoma cells and epidermal keratinocytes. This study investigated changes in the skin in the K5-Brn2 transgenic mouse, which overexpresses Brn2 and contains the keratin 5 promotor. Interestingly, the volume of eccrine sweat glands was reduced markedly in the K5-Brn2 transgenic mouse compared with the wild-type, while the expression of aquaporin 5, important molecule in sweat secretion, was increased in each sweat gland cell, probably to compensate for the reduction in gland development. However, sweating response to a pilocarpine injection in the hind paw was significantly decreased in the K5-Brn2 transgenic mouse compared with the wild-type. The paw epidermis was thicker in the K5-Brn2 transgenic mouse compared with the wild-type. Taken together, eccrine sweat gland development and sweat secretion were suppressed markedly in the K5-Brn2 transgenic mouse. These results may be associated with dominant development of the epidermis by Brn2 overexpression in the paw skin.
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http://dx.doi.org/10.1016/j.bbrc.2017.06.138DOI Listing
August 2017

Low-Dose Systemic Methotrexate Therapy for Recalcitrant Alopecia Areata.

Ann Dermatol 2017 Jun 11;29(3):263-267. Epub 2017 May 11.

Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.

Background: Alopecia areata (AA) is an autoimmune skin disease difficult to manage and treat. The pathogenesis of AA features a T-cell-associated autoimmune process, and systemic immunosuppressive therapy is prescribed widely for AA.

Objective: To evaluate the efficacy and tolerance of systemic low-dose methotrexate (LD-MTX) therapy in treatment of recalcitrant AA multiplex.

Methods: In a retrospective, non-controlled study, we evaluated 29 patients with recalcitrant AA treated with LD-MTX and assessed the therapeutic response according to severity of disease, disease duration, cumulative dose of MTX, and drug safety.

Results: MTX was administered twice weekly, and the mean maximum weekly dose was 14.48 mg. The response was A5 (regrowth=100.0%) in 14 (48.3%) patients and A4 (regrowth of 75%~90%) in 12 (41.4%) patients. Three patients had poor response to LD-MTX treatment (A2: n=2 [6.9%], A1: n=1 [3.4%]). All three of the patients showing a poor response had disease durations exceeding 24 months. Relapse was observed in 31% of patients with more than 75% regrowth. Common side-effects were elevated liver enzyme levels and gastrointestinal discomfort.

Conclusion: LD-MTX appears to be an effective and well-tolerated treatment for recalcitrant AA multiplex.
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http://dx.doi.org/10.5021/ad.2017.29.3.263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438930PMC
June 2017

Treatment of Recalcitrant Pyoderma Gangrenosum with Ulcerative Colitis by Adalimumab Injection.

Ann Dermatol 2017 Apr 24;29(2):260-262. Epub 2017 Mar 24.

Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.

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http://dx.doi.org/10.5021/ad.2017.29.2.260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383766PMC
April 2017

Primary Cutaneous Aspergillosis after Tattoo Removal Using a 1,064-nm Q-Switched Nd:YAG Laser in an Immunocompetent Patient.

Ann Dermatol 2017 Apr 24;29(2):241-243. Epub 2017 Mar 24.

Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.

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http://dx.doi.org/10.5021/ad.2017.29.2.241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5383758PMC
April 2017