Publications by authors named "Jennifer A Doudna"

100Publications

DNA capture by a CRISPR-Cas9-guided adenine base editor.

Science 2020 07;369(6503):566-571

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

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http://dx.doi.org/10.1126/science.abb1390DOI Listing
July 2020

Engineering of monosized lipid-coated mesoporous silica nanoparticles for CRISPR delivery.

Acta Biomater 2020 Jul 21. Epub 2020 Jul 21.

Department of Chemical and Biological Engineering, University of New Mexico, Albuquerque, NM, United States; Advanced Materials Laboratory, Sandia National Laboratories, Albuquerque, NM, United States; Department of Internal Medicine, Molecular Medicine, University of New Mexico Health Science Center, Albuquerque, NM, United States. Electronic address:

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http://dx.doi.org/10.1016/j.actbio.2020.07.027DOI Listing
July 2020

CRISPR-CasΦ from huge phages is a hypercompact genome editor.

Science 2020 07;369(6501):333-337

Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.

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http://dx.doi.org/10.1126/science.abb1400DOI Listing
July 2020

A scoutRNA Is Required for Some Type V CRISPR-Cas Systems.

Mol Cell 2020 Aug 8;79(3):416-424.e5. Epub 2020 Jul 8.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Molecular Biophysics & Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Gladstone Institutes, University of California, San Francisco, San Francisco, CA 94114, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molcel.2020.06.022DOI Listing
August 2020

CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks.

Elife 2020 06 10;9. Epub 2020 Jun 10.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.

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http://dx.doi.org/10.7554/eLife.55143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286691PMC
June 2020

Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity.

Nat Biotechnol 2020 Jul 16;38(7):883-891. Epub 2020 Mar 16.

Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.

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http://dx.doi.org/10.1038/s41587-020-0453-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357821PMC
July 2020

Machine learning predicts new anti-CRISPR proteins.

Nucleic Acids Res 2020 05;48(9):4698-4708

Department of Computer and Information Sciences, Pakistan Institute of Engineering and Applied Sciences (PIEAS), PO Nilore, Islamabad, Pakistan.

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http://dx.doi.org/10.1093/nar/gkaa219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229843PMC
May 2020

Potent CRISPR-Cas9 inhibitors from genomes.

Proc Natl Acad Sci U S A 2020 03 10;117(12):6531-6539. Epub 2020 Mar 10.

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720;

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http://dx.doi.org/10.1073/pnas.1917668117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104187PMC
March 2020

Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling.

Proc Natl Acad Sci U S A 2020 03 2;117(11):5853-5860. Epub 2020 Mar 2.

Department of Bioengineering, Stanford University, Stanford, CA 94305;

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http://dx.doi.org/10.1073/pnas.1913445117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084090PMC
March 2020

The promise and challenge of therapeutic genome editing.

Nature 2020 02 12;578(7794):229-236. Epub 2020 Feb 12.

Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.

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http://dx.doi.org/10.1038/s41586-020-1978-5DOI Listing
February 2020

Knocking out barriers to engineered cell activity.

Science 2020 02 6;367(6481):976-977. Epub 2020 Feb 6.

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

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http://dx.doi.org/10.1126/science.aba9844DOI Listing
February 2020

Reply to Nathamgari et al.: Nanopore electroporation for intracellular delivery of biological macromolecules.

Proc Natl Acad Sci U S A 2019 11 29;116(46):22911. Epub 2019 Oct 29.

Department of Chemistry, University of California, Berkeley, CA 94720;

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http://dx.doi.org/10.1073/pnas.1912715116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859330PMC
November 2019

Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble.

Nat Commun 2019 07 5;10(1):3001. Epub 2019 Jul 5.

Department of Molecular and Cell Biology, University of California, Berkeley, CA, 94720, USA.

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http://dx.doi.org/10.1038/s41467-019-10780-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611850PMC
July 2019

Candidate Phyla Radiation Roizmanbacteria From Hot Springs Have Novel and Unexpectedly Abundant CRISPR-Cas Systems.

Front Microbiol 2019 3;10:928. Epub 2019 May 3.

Department of Earth and Planetary Sciences, University of California, Berkeley, Berkeley, CA, United States.

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http://dx.doi.org/10.3389/fmicb.2019.00928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509639PMC
May 2019

Deciphering Off-Target Effects in CRISPR-Cas9 through Accelerated Molecular Dynamics.

ACS Cent Sci 2019 Apr 7;5(4):651-662. Epub 2019 Mar 7.

Department of Bioengineering, Bourns College of Engineering, University of California Riverside, 900 University Avenue, Riverside, California 92521, United States.

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http://pubs.acs.org/doi/10.1021/acscentsci.9b00020
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http://dx.doi.org/10.1021/acscentsci.9b00020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487449PMC
April 2019

A Unified Resource for Tracking Anti-CRISPR Names.

CRISPR J 2018 10;1:304-305

15 Department of Microbiology and Immunology, Montana State University, Bozeman, Montana.

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http://dx.doi.org/10.1089/crispr.2018.0043DOI Listing
October 2018

Broad-spectrum enzymatic inhibition of CRISPR-Cas12a.

Nat Struct Mol Biol 2019 04 1;26(4):315-321. Epub 2019 Apr 1.

Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.

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http://dx.doi.org/10.1038/s41594-019-0208-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449189PMC
April 2019

Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules.

Proc Natl Acad Sci U S A 2019 04 28;116(16):7899-7904. Epub 2019 Mar 28.

Department of Chemistry, University of California, Berkeley, CA 94720;

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http://dx.doi.org/10.1073/pnas.1818553116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475394PMC
April 2019

Spacer Acquisition Rates Determine the Immunological Diversity of the Type II CRISPR-Cas Immune Response.

Cell Host Microbe 2019 02 29;25(2):242-249.e3. Epub 2019 Jan 29.

Laboratory of Bacteriology, The Rockefeller University, New York, NY 10065, USA; Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA. Electronic address:

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http://dx.doi.org/10.1016/j.chom.2018.12.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640137PMC
February 2019

A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.

Mol Cell 2019 02 29;73(4):727-737.e3. Epub 2019 Jan 29.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; MBIB Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Gladstone Institutes, San Francisco, CA 94158, USA. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S10972765183106
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http://dx.doi.org/10.1016/j.molcel.2018.12.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6386590PMC
February 2019

Temperature-Responsive Competitive Inhibition of CRISPR-Cas9.

Mol Cell 2019 02 27;73(3):601-610.e5. Epub 2018 Dec 27.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, Berkeley, CA 94704, USA; Howard Hughes Medical Institute, Berkeley; MBIB Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Gladstone Institutes, San Francisco, CA 94158, USA. Electronic address:

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https://linkinghub.elsevier.com/retrieve/pii/S10972765183098
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http://dx.doi.org/10.1016/j.molcel.2018.11.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480404PMC
February 2019

Systematic discovery of natural CRISPR-Cas12a inhibitors.

Science 2018 10 6;362(6411):236-239. Epub 2018 Sep 6.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.

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http://dx.doi.org/10.1126/science.aau5138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6185749PMC
October 2018

CRISPR-Cas guides the future of genetic engineering.

Science 2018 08;361(6405):866-869

Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.

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http://www.sciencemag.org/lookup/doi/10.1126/science.aat5011
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http://dx.doi.org/10.1126/science.aat5011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6455913PMC
August 2018

RNA Binding and HEPN-Nuclease Activation Are Decoupled in CRISPR-Cas13a.

Cell Rep 2018 07;24(4):1025-1036

Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA; Center for RNA Biology, University of Rochester, Rochester, NY 14642, USA. Electronic address:

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http://dx.doi.org/10.1016/j.celrep.2018.06.105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085867PMC
July 2018

Applications of CRISPR-Cas Enzymes in Cancer Therapeutics and Detection.

Trends Cancer 2018 07 13;4(7):499-512. Epub 2018 Jun 13.

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA; MBIB Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, CA 94720, USA. Electronic address:

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http://dx.doi.org/10.1016/j.trecan.2018.05.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299457PMC
July 2018

Programmable RNA recognition using a CRISPR-associated Argonaute.

Proc Natl Acad Sci U S A 2018 03 12;115(13):3368-3373. Epub 2018 Mar 12.

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720;

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http://dx.doi.org/10.1073/pnas.1717725115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5879674PMC
March 2018

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity.

Science 2018 04 15;360(6387):436-439. Epub 2018 Feb 15.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.

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http://dx.doi.org/10.1126/science.aar6245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628903PMC
April 2018

Spotlight: A Conversation with Laura Kiessling and Jennifer Doudna.

ACS Chem Biol 2018 Feb;13(2):290-295

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http://dx.doi.org/10.1021/acschembio.8b00108DOI Listing
February 2018

RNA-dependent RNA targeting by CRISPR-Cas9.

Elife 2018 01 5;7. Epub 2018 Jan 5.

Department of Molecular and Cell Biology, University of California, Berkeley, United States.

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http://dx.doi.org/10.7554/eLife.32724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796797PMC
January 2018

Widespread Translational Remodeling during Human Neuronal Differentiation.

Cell Rep 2017 Nov;21(7):2005-2016

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143, USA; Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address:

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http://dx.doi.org/10.1016/j.celrep.2017.10.095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5759054PMC
November 2017

A thermostable Cas9 with increased lifetime in human plasma.

Nat Commun 2017 11 10;8(1):1424. Epub 2017 Nov 10.

Department of Molecular and Cell Biology, University of California, Berkeley, CA, 94720, USA.

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http://dx.doi.org/10.1038/s41467-017-01408-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5681539PMC
November 2017

Genomes in Focus: Development and Applications of CRISPR-Cas9 Imaging Technologies.

Angew Chem Int Ed Engl 2018 04 28;57(16):4329-4337. Epub 2018 Feb 28.

Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA.

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http://dx.doi.org/10.1002/anie.201709201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014596PMC
April 2018

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.

Nature 2017 10 20;550(7676):407-410. Epub 2017 Sep 20.

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.

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http://dx.doi.org/10.1038/nature24268DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5918688PMC
October 2017

Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme.

Nat Struct Mol Biol 2017 Oct 11;24(10):825-833. Epub 2017 Sep 11.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.

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http://dx.doi.org/10.1038/nsmb.3466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5961731PMC
October 2017

A Broad-Spectrum Inhibitor of CRISPR-Cas9.

Cell 2017 Sep 24;170(6):1224-1233.e15. Epub 2017 Aug 24.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Biophysics Graduate Group, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA; MBIB Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address:

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http://dx.doi.org/10.1016/j.cell.2017.07.037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875921PMC
September 2017

A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9.

Sci Adv 2017 08 4;3(8):eaao0027. Epub 2017 Aug 4.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.

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http://dx.doi.org/10.1126/sciadv.aao0027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547770PMC
August 2017

Structures of the CRISPR genome integration complex.

Science 2017 09 20;357(6356):1113-1118. Epub 2017 Jul 20.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.

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http://dx.doi.org/10.1126/science.aao0679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748385PMC
September 2017

Disabling Cas9 by an anti-CRISPR DNA mimic.

Sci Adv 2017 07 12;3(7):e1701620. Epub 2017 Jul 12.

Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.

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http://dx.doi.org/10.1126/sciadv.1701620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507636PMC
July 2017

DNA recognition by an RNA-guided bacterial Argonaute.

PLoS One 2017 17;12(5):e0177097. Epub 2017 May 17.

Biophysics Graduate Group, University of California, Berkeley, California, United States of America.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0177097PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435312PMC
September 2017

RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes.

Mol Cell 2017 May;66(3):373-383.e3

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, CA 94720, USA; MBIB Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molcel.2017.04.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5999320PMC
May 2017

CRISPR-Cas9 Structures and Mechanisms.

Annu Rev Biophys 2017 05 30;46:505-529. Epub 2017 Mar 30.

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720; email: ,

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http://www.annualreviews.org/doi/10.1146/annurev-biophys-062
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http://dx.doi.org/10.1146/annurev-biophys-062215-010822DOI Listing
May 2017

RNA-based recognition and targeting: sowing the seeds of specificity.

Nat Rev Mol Cell Biol 2017 04 15;18(4):215-228. Epub 2017 Feb 15.

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.

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http://dx.doi.org/10.1038/nrm.2016.174DOI Listing
April 2017

RNA and DNA Targeting by a Reconstituted Thermus thermophilus Type III-A CRISPR-Cas System.

PLoS One 2017 23;12(1):e0170552. Epub 2017 Jan 23.

Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America.

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0170552PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5256923PMC
August 2017

Mutations in Cas9 Enhance the Rate of Acquisition of Viral Spacer Sequences during the CRISPR-Cas Immune Response.

Mol Cell 2017 Jan 22;65(1):168-175. Epub 2016 Dec 22.

Laboratory of Bacteriology, The Rockefeller University, New York, NY 10065, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molcel.2016.11.031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5218886PMC
January 2017

Cornerstones of CRISPR-Cas in drug discovery and therapy.

Nat Rev Drug Discov 2017 02 23;16(2):89-100. Epub 2016 Dec 23.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA.

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http://dx.doi.org/10.1038/nrd.2016.238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5459481PMC
February 2017

New CRISPR-Cas systems from uncultivated microbes.

Nature 2017 02 22;542(7640):237-241. Epub 2016 Dec 22.

Department of Earth and Planetary Sciences, University of California, Berkeley, California 94720, USA.

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http://dx.doi.org/10.1038/nature21059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5300952PMC
February 2017

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection.

Nature 2016 Oct 26;538(7624):270-273. Epub 2016 Sep 26.

Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.

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http://dx.doi.org/10.1038/nature19802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576363PMC
October 2016

Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9.

Nat Commun 2016 09 14;7:12778. Epub 2016 Sep 14.

Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

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http://dx.doi.org/10.1038/ncomms12778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5027287PMC
September 2016

Applications of CRISPR technologies in research and beyond.

Nat Biotechnol 2016 8;34(9):933-941. Epub 2016 Sep 8.

Howard Hughes Medical Institute, Innovative Genomics Initiative, Department of Molecular and Cell Biology, University of California, Berkeley, California, USA.

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http://dx.doi.org/10.1038/nbt.3659DOI Listing
September 2016

Protecting genome integrity during CRISPR immune adaptation.

Nat Struct Mol Biol 2016 Oct 5;23(10):876-883. Epub 2016 Sep 5.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.

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http://dx.doi.org/10.1038/nsmb.3289DOI Listing
October 2016

DNA Targeting by a Minimal CRISPR RNA-Guided Cascade.

Mol Cell 2016 09;63(5):840-51

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Molecular Biophysics and Integrative Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address:

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http://www.cell.com/molecular-cell/pdf/S1097-2765(16)30414-2
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http://dx.doi.org/10.1016/j.molcel.2016.07.027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5111854PMC
September 2016

Reconstitution of selective HIV-1 RNA packaging in vitro by membrane-bound Gag assemblies.

Elife 2016 06 25;5. Epub 2016 Jun 25.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.

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http://dx.doi.org/10.7554/eLife.14663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4946900PMC
June 2016

CRISPR Immunological Memory Requires a Host Factor for Specificity.

Mol Cell 2016 06 19;62(6):824-833. Epub 2016 May 19.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Initiative, University of California, Berkeley, Berkeley, CA 94720, USA; Center for RNA Systems Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Molecular Biophysics and Integrated Bioimaging, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molcel.2016.04.027DOI Listing
June 2016

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch.

Nat Biotechnol 2016 06 2;34(6):646-51. Epub 2016 May 2.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.

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http://dx.doi.org/10.1038/nbt.3528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900928PMC
June 2016

Nucleosome breathing and remodeling constrain CRISPR-Cas9 function.

Elife 2016 04 28;5. Epub 2016 Apr 28.

Center for Systems and Synthetic Biology, University of California, San Francisco, San Francisco, United States.

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http://dx.doi.org/10.7554/eLife.13450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880442PMC
April 2016

A bacterial Argonaute with noncanonical guide RNA specificity.

Proc Natl Acad Sci U S A 2016 Apr 30;113(15):4057-62. Epub 2016 Mar 30.

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720; Biophysics Graduate Group, University of California, Berkeley, CA 94720; Howard Hughes Medical Institute, University of California, Berkeley, CA 94720; Center for RNA Systems Biology, University of California, Berkeley, CA 94720; Innovative Genomics Initiative, University of California, Berkeley, CA 94720; Department of Chemistry, University of California, Berkeley, CA 94720

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http://dx.doi.org/10.1073/pnas.1524385113DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839417PMC
April 2016

Programmable RNA Tracking in Live Cells with CRISPR/Cas9.

Cell 2016 Apr 17;165(2):488-96. Epub 2016 Mar 17.

Department of Cellular and Molecular Medicine and Institute for Genomic Medicine, University of California, San Diego, La Jolla, CA 92037, USA; Materials Science and Engineering Graduate Program, University of California, San Diego, La Jolla, CA 92093, USA; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 1190777, Singapore. Electronic address:

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http://www.cell.com/cell/pdf/S0092-8674(16)30204-5.pdf
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http://dx.doi.org/10.1016/j.cell.2016.02.054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4826288PMC
April 2016

Chemical and Biophysical Modulation of Cas9 for Tunable Genome Engineering.

ACS Chem Biol 2016 Mar 9;11(3):681-8. Epub 2016 Feb 9.

Department of Molecular and Cell Biology, University of California, Berkeley , Berkeley, California 94720, United States.

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http://dx.doi.org/10.1021/acschembio.5b01019DOI Listing
March 2016

Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage.

Science 2016 Feb 14;351(6275):867-71. Epub 2016 Jan 14.

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA. California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA. Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA. Department of Chemistry, University of California, Berkeley, CA 94720, USA. Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

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http://dx.doi.org/10.1126/science.aad8282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5111852PMC
February 2016

Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering.

Cell 2016 Jan;164(1-2):29-44

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute HHMI, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Center for RNA Systems Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Initiative, University of California, Berkeley, Berkeley, CA 94720, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, Berkeley, CA 94720, USA. Electronic address:

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http://dx.doi.org/10.1016/j.cell.2015.12.035DOI Listing
January 2016

Tunable protein synthesis by transcript isoforms in human cells.

Elife 2016 Jan 6;5. Epub 2016 Jan 6.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States.

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http://dx.doi.org/10.7554/eLife.10921DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764583PMC
January 2016

Genome editing: the end of the beginning.

Genome Biol 2015 Dec 23;16:292. Epub 2015 Dec 23.

Department of Biomedical Engineering, Duke University, Durham, North Carolina, 27708, USA.

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http://dx.doi.org/10.1186/s13059-015-0860-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4699356PMC
December 2015

Analog sensitive chemical inhibition of the DEAD-box protein DDX3.

Protein Sci 2016 Mar 26;25(3):638-49. Epub 2015 Dec 26.

Department of Molecular and Cell Biology, University of California, Berkeley, California.

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http://dx.doi.org/10.1002/pro.2857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815421PMC
March 2016

Get in LINE: Competition for Newly Minted Retrotransposon Proteins at the Ribosome.

Mol Cell 2015 Dec;60(5):712-714

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Initiative, University of California, Berkeley, Berkeley, CA 94720, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molcel.2015.11.014DOI Listing
December 2015

Autoinhibitory Interdomain Interactions and Subfamily-specific Extensions Redefine the Catalytic Core of the Human DEAD-box Protein DDX3.

J Biol Chem 2016 Jan 23;291(5):2412-21. Epub 2015 Nov 23.

From the Department of Molecular and Cell Biology, Howard Hughes Medical Institute, Department of Chemistry, and Innovative Genomics Initiative, University of California, Berkeley, California 94720, the Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, and

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http://dx.doi.org/10.1074/jbc.M115.700625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4732223PMC
January 2016

Dynamics of CRISPR-Cas9 genome interrogation in living cells.

Science 2015 Nov;350(6262):823-6

Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. Transcriptional Imaging Consortium, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. Li Ka Shing Biomedical and Health Sciences Center, University of California, Berkeley, CA, USA.

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http://dx.doi.org/10.1126/science.aac6572DOI Listing
November 2015

Single-Stranded DNA Cleavage by Divergent CRISPR-Cas9 Enzymes.

Mol Cell 2015 Nov;60(3):398-407

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Center for RNA Systems Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Innovative Genomics Initiative, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molcel.2015.10.030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4636735PMC
November 2015

Conformational control of DNA target cleavage by CRISPR-Cas9.

Nature 2015 Nov 28;527(7576):110-3. Epub 2015 Oct 28.

Department of Chemistry, University of California, Berkeley, California 94720, USA.

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http://dx.doi.org/10.1038/nature15544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859810PMC
November 2015

Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System.

Cell 2015 Nov;163(4):854-65

Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA. Electronic address:

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http://dx.doi.org/10.1016/j.cell.2015.10.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4636941PMC
November 2015

Foreign DNA capture during CRISPR-Cas adaptive immunity.

Nature 2015 Nov 21;527(7579):535-8. Epub 2015 Oct 21.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA.

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http://dx.doi.org/10.1038/nature15760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4662619PMC
November 2015

Ancient Origin of cGAS-STING Reveals Mechanism of Universal 2',3' cGAMP Signaling.

Mol Cell 2015 Sep 20;59(6):891-903. Epub 2015 Aug 20.

Department of Molecular & Cell Biology, University of California, Berkeley, CA 94720, USA; Cancer Research Laboratory, University of California, Berkeley, CA 94720, USA; Howard Hughes Medical Institute (HHMI), University of California, Berkeley, CA 94720, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molcel.2015.07.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575873PMC
September 2015

Generation of knock-in primary human T cells using Cas9 ribonucleoproteins.

Proc Natl Acad Sci U S A 2015 Aug 27;112(33):10437-42. Epub 2015 Jul 27.

Diabetes Center, University of California, San Francisco, CA 94143; Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, CA 94143; Innovative Genomics Initiative, University of California, Berkeley, CA 94720;

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http://dx.doi.org/10.1073/pnas.1512503112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547290PMC
August 2015

STRUCTURAL BIOLOGY. A Cas9-guide RNA complex preorganized for target DNA recognition.

Science 2015 Jun;348(6242):1477-81

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA. Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA. California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA. Department of Chemistry, University of California, Berkeley, CA 94720, USA. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Innovative Genomics Initiative, University of California, Berkeley, CA 94720, USA.

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http://dx.doi.org/10.1126/science.aab1452DOI Listing
June 2015

Expanding the Biologist's Toolkit with CRISPR-Cas9.

Mol Cell 2015 May;58(4):568-74

Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Initiative, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molcel.2015.02.032DOI Listing
May 2015

Structural biology. Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning.

Science 2015 May 2;348(6234):581-5. Epub 2015 Apr 2.

Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA. California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA. Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA. Department of Chemistry, University of California, Berkeley, CA 94720, USA. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

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http://dx.doi.org/10.1126/science.aaa4535DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582657PMC
May 2015

Biotechnology. A prudent path forward for genomic engineering and germline gene modification.

Science 2015 Apr 19;348(6230):36-8. Epub 2015 Mar 19.

Innovative Genomics Initiative, University of California, Berkeley, 188 Li Ka Shing Center, Berkeley, CA 94720-3370, USA. UCSF School of Medicine, 600 16th Street, San Francisco, CA 94158, USA.

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http://dx.doi.org/10.1126/science.aab1028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394183PMC
April 2015