Publications by authors named "Jeancarlo Pereira Dos Anjos"

7 Publications

  • Page 1 of 1

A Simple Method for Evaluating the Bioactive Phenolic Compounds' Presence in Brazilian Craft Beers.

Molecules 2021 Aug 4;26(16). Epub 2021 Aug 4.

Centro Universitário SENAI CIMATEC, Avenida Orlando Gomes, 1845-Piatã, Salvador 41650-010, BA, Brazil.

There are a significant number of analytical methodologies employing different techniques to determine phenolic compounds in beverages. However, these methods employ long sample preparation processes and great time consumption. The aim of this paper was the development of a simple method for evaluating the phenolic compounds' presence in Brazilian craft beers without a previous extraction step. Catechin, caffeic acid, epicatechin, -coumaric acid, hydrated rutin, -ferulic acid, quercetin, kaempferol, and formononetin were analyzed in fifteen different craft beers. The method showed good linearity (R ≥ 0.9966). The limit of detection ranged from 0.08 to 0.83 mg L, and limits of quantification were between 0.27 and 2.78 mg L. The method showed a satisfactory precision (RSD ≤ 16.2%). A good accuracy was obtained by the proposed method for all phenolic compounds in craft beer (68.6% ˂ accuracy ˂ 112%). Catechin showed higher concentrations (up to 124.8 mg L) in the samples, followed by epicatechin (up to 51.1 mg L) and caffeic acid (up to 8.13 mg L). Rutin and formononetin were observed in all analyzed samples (0.52 mg L to 2.40 mg L), and kaempferol was less present in the samples. The presence of plant origin products was determinant for the occurrence of the highest concentrations of phenolic compounds in Brazilian craft beers.
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http://dx.doi.org/10.3390/molecules26164716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399525PMC
August 2021

Exploratory analysis of the presence of 14 carbonyl compounds in bottled mineral water in polyethylene terephthalate (PET) containers.

Food Chem 2021 Dec 26;365:130475. Epub 2021 Jun 26.

Centro Interdisciplinar de Energia e Ambiente - CIEnAm, Universidade Federal da Bahia, Ondina, Salvador, BA 40170-290, Brazil; Instituto Nacional de Ciência e Tecnologia em Energia e Ambiente - INCT E&A, UFBA, Salvador, BA 40170-290, Brazil; Centro Universitário SENAI CIMATEC, Av. Orlando Gomes, 1845 - Piatã, CEP41650-010 Salvador, BA, Brazil. Electronic address:

Carbonyl compounds (CCs) can migrate from bottles to mineral water because of plastic degradation. An exploratory analysis of the presence a significant number of CCs (14) in bottled mineral water with and without gas in polyethylene terephthalate (PET) containers was performed using ultra-fast liquid chromatography coupled to mass spectrometry (UFLC-MS). The data from the analysis was submitted to chemometric treatment (principal component analysis, PCA). Formaldehyde, acetaldehyde, and benzaldehyde were found in all samples (0.07-125 ng mL). Acrolein and acetone were present in 81% and 75% of the samples, respectively. The concentration of acrolein in carbonated water was up to 3.8 times greater than that measured in non-carbonated water (0.07-0.44 ± 0.01 ng mL). PCA analysis showed that gasification can influence the composition of CCs present in mineral water and that the plastic material of the bottles is a likely source of CCs. In addition, benzaldehyde levels may be associated with the use of recycled materials.
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http://dx.doi.org/10.1016/j.foodchem.2021.130475DOI Listing
December 2021

Supercritical Extraction of Red Propolis: Operational Conditions and Chemical Characterization.

Molecules 2020 Oct 20;25(20). Epub 2020 Oct 20.

School of Pharmacy, Federal University of Bahia (UFBA), Barão de Jeremoabo Street, 147, Salvador 40110-100, Bahia, Brazil.

The objective of this study was to determine the best operational conditions for obtaining red propolis extract with high antioxidant potential through supercritical fluid extraction (SFE) technology, using carbon dioxide (CO) as the supercritical fluid and ethanol as the cosolvent. The following parameters were studied: overall extraction curve, S/F (mass of CO/mass of sample), cosolvent percentage (0, 1, 2 and 4%) and global yield isotherms as a function of different pressures (250, 350 and 450 bar) and temperatures (31.7, 40 and 50 °C). Within the investigated parameters, the best conditions found were an S/F of 131 and the use of ethanol at the highest concentration (4% /), which resulted in higher extract yields and higher content of antioxidant compounds. Formononetin, the main biomarker of red propolis, was the compound found at the highest amounts in the extracts. As expected, the temperature and pressure conditions also influenced the process yield, with 350 bar and 40 °C being the best conditions for obtaining bioactive compounds from a sample of red propolis. The novel results for red propolis found in this study show that it is possible to obtain extracts with high antioxidant potential using a clean technology under the defined conditions.
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http://dx.doi.org/10.3390/molecules25204816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587948PMC
October 2020

Determination of free- and bound-carbonyl compounds in airborne particles by ultra-fast liquid chromatography coupled to mass spectrometry.

Talanta 2020 Sep 14;217:121033. Epub 2020 Apr 14.

Centro Interdisciplinar de Energia e Ambiente - CIEnAm, Universidade Federal da Bahia, Ondina, Salvador, BA, 40170-290, Brazil; Instituto Nacional de Ciência e Tecnologia em Energia e Ambiente - INCT E&A, UFBA, Salvador, BA, 40170-290, Brazil; Centro Universitário SENAI CIMATEC, 40170-010, Salvador, BA, Brazil. Electronic address:

This study presents the development and application of a new analytical methodology for determination of free- and bound-carbonyl compounds (CC) (as the CC themselves and as the hydroxyalkylsulfonic acids - HASA, respectively) in airborne particles. Free- and bound-CC determination were done through reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) and analysis by UFLC-MS. The method was successfully validated, showing good figures for linearity (R ≥ 0.9937), sensibility (3 fg ˂ LOD ˂ 20 fg for methacrolein and heptanal, respectively) and repeatability (5.9% ˂ RSD ˂ 13%). The proposed method was successfully applied in real samples of inhalable atmospheric particulate matter (PM) and urban dust certified reference material (SRM 1649 b). The main CC determined in the SRM 1649 b was formaldehyde (75.4 μg g in the free form, and 1898 μg g in the bound form). In addition, for the bound-CC form (HASA), concentrations were determined for acetaldehyde (60.3 μg g), acetone (20.5 μg g), acrolein (9.15 μg g), propionaldehyde (17.1 μg g) and valeraldehyde (12.2 μg g). For PM samples, formaldehyde (148 μg g) and acetaldehyde (28.9 μg g) were quantified as free aldehydes and as HASA (hydroxymethanelsulfonic acid and hydroxyethanesulfonic acid were 432 μg g and 211 μg g, respectively). Other bound-CC were, on average, within 19.2 μg g (acrolein) and 62.1 μg g (valeraldehyde). For all samples, acetone, acrolein, propionaldehyde and valeraldehyde were quantified only as HASA (bound-CC). Therefore, we could identify and quantify six carbonyl compounds using the proposed method. It is worth mentioning the hydrolysis step was crucial for the correct quantification of the HASAs. This was, in turn, what enabled the quantification of a greater number of analytes in the airborne samples. Hence, this procedure was found to be comprehensive, precise, accurate and suitable to be employed for determination of free-CC and HASA (bound-CC) in atmospheric particulate samples.
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http://dx.doi.org/10.1016/j.talanta.2020.121033DOI Listing
September 2020

Effect of Experimental Parameters on the Extraction of Grape Seed Oil Obtained by Low Pressure and Supercritical Fluid Extraction.

Molecules 2020 Apr 2;25(7). Epub 2020 Apr 2.

Federal University of Bahia, Bahia, Salvador 40170-115, Brazil.

Grape seeds are an important byproduct from the grape process. The objective of this work was to evaluate the effect of experimental parameters (temperature and time of pretreatment with ultrasound) to obtain grape seed oil using low pressure (Soxhlet-Sox and Bligh Dyer-BD) and high pressure (supercritical carbon dioxide-SFE) methods. The best condition for pretreatment of samples was 30 min of sonication at 30 °C before extraction by Sox or BD. Ultrasound pretreatment was efficient to increase oil extraction yield by 32.10 (Sox), 20.31 (BD) and 12.54% (SFE), depending on the extraction method used as well as, and certainly influenced the total phenolic concentration in 311 (Sox), 234 (BD), and 184 (SFE)%. Ten fatty acids were identified in the oils, the major ones being 18:2ω-6cis (linoleic 52.39%-63.12%), 16:0 (palmitic 20.22%-26.80%) and 18:0 (stearic 8.52%-13.68%). The highest epicatechin concentration was identified in the BD sample: 30-30 (150.49 ± 5.98mg/kg), which presented a concentration of ≥3 times compared to the control (56.68 ± 1.81mg/kg). Ultrasound pretreatment also contributed positively (56% and 99% increase) in the α-tocopherol content of the SFE: 30-30 and BD: 30-30 samples, respectively. The results indicate that the ultrasound pretreatment is a suitable technology to improve the quality of the oil from the grape seed.
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http://dx.doi.org/10.3390/molecules25071634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180707PMC
April 2020

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction.

PLoS One 2019 5;14(7):e0219063. Epub 2019 Jul 5.

University Center SENAI/CIMATEC, National Service of Industrial Learning - SENAI, Heath Institute of Technology (ITS CIMATEC), Salvador, Bahia, Brazil.

Propolis is a complex mixture of resinous and balsamic material collected from the exudates of plants, shoots, and leaves by bees. This study evaluated red propolis extracts obtained by conventional (ethanolic) extraction and ultrasound-assisted extraction of six samples from different regions of northeastern Brazil. The total phenolic compounds and flavonoids, in vitro antioxidant activity, concentration of formononetin and kaempferol and the cytotoxicity against four human tumor cell lines were determined for all twelve obtained extracts. Significant variations in the levels of the investigated compounds were identified in the red propolis extracts, confirming that the chemical composition varied according to the sampling region. The extraction method used also influenced the resulting propolis compounds. The highest concentration of the compounds of interest and the highest in vitro antioxidant activity were exhibited by the extracts obtained from samples from state of Alagoas. Formononetin and kaempferol were identified in all samples. The highest formononetin concentrations were identified in extracts obtained by ultrasound, thus indicating a greater selectivity for the extraction of this compound by this method. Regarding cytotoxic activity, for the HCT-116 line, all of the extracts showed an inhibition of greater than 90%, whereas for the HL-60 and PC3 lines, the minimum identified was 80%. In general, there was no significant difference (p>0.05) in the antiproliferative potential when comparing the extraction methods. The results showed that the composition of Brazilian red propolis varies significantly depending on the geographical origin and that the method used influences the resulting compounds that are present in propolis. However, regardless of the geographical origin and the extraction method used, all the red propolis samples studied presented great biological potential and high antioxidant activity. Furthermore, the ultrasound-assisted method can be efficiently applied to obtain extracts of red propolis more quickly and with high concentration of biomarkers of interest.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0219063PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611595PMC
February 2020

Determination of ethyl carbamate in cachaça produced from copper stills by HPLC.

Food Chem 2013 Jun 29;138(2-3):1233-8. Epub 2012 Nov 29.

Departamento de Ciência dos Alimentos, Universidade Federal de Lavras, Campus Universitário CP 3037, CEP 37200-000 Lavras, MG, Brazil.

Ethyl carbamate (EC) is a common substance in fermented foods and drinks, and its quantification is important because of its carcinogenic nature and its usually presence in alcoholic beverages. The present work involved the development and validation of an analytical method for the evaluation of EC in cachaça by HPLC-FLD after previous derivatization with xanthydrol. The method presented a mean recovery of 94.88%, an intra-day precision of 4.19% (30.0 μgL(-1)) and 3.32% (75.0 μgL(-1)), a coefficient of determination (r(2)) equal to 0.9985, and limits of detection and quantification equal to 6.39 and 21.32 μgL(-1), respectively. The results show that the analytical method is accurate, reproducible and linear over the concentration range from 5.0 to 160 μg of EC per litre. The method was applied to the analysis of EC in cachaça, the analyses being rapid and efficient.
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http://dx.doi.org/10.1016/j.foodchem.2012.11.048DOI Listing
June 2013
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