Publications by authors named "Jean Louis Marty"

120 Publications

Analysis of Recent Bio-/Nanotechnologies for Coronavirus Diagnosis and Therapy.

Sensors (Basel) 2021 Feb 20;21(4). Epub 2021 Feb 20.

Laboratoire BAE, Université de Perpignan Via domitia, 66860 Perpignan, France.

Despite barrier measures and physical distancing tailored by the populations worldwide, coronavirus continues to spread causing severe health and social-economic problems. Therefore, researchers are focusing on developing efficient detection and therapeutic platforms for SARS-CoV2. In this context, various biotechnologies, based on novel molecules targeting the virus with high specificity and affinity, have been described. In parallel, new approaches exploring nanotechnology have been proposed for enhancing treatments and diagnosis. We discuss in the first part of this review paper, the different biosensing and rapid tests based on antibodies, nucleic acids and peptide probes described since the beginning of the pandemic. Furthermore, given their numerous advantages, the contribution of nanotechnologies is also highlighted.
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http://dx.doi.org/10.3390/s21041485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7924586PMC
February 2021

An Overview of Optical and Electrochemical Sensors and Biosensors for Analysis of Antioxidants in Food during the Last 5 Years.

Sensors (Basel) 2021 Feb 7;21(4). Epub 2021 Feb 7.

Faculty of Sciences, University of Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX 9, France.

Antioxidants are a group of healthy substances which are useful to human health because of their antihistaminic, anticancer, anti-inflammatory activity and inhibitory effect on the formation and the actions of reactive oxygen species. Generally, they are phenolic complexes present in plant-derived foods. Due to the valuable nutritional role of these mixtures, analysis and determining their amount in food is of particular importance. In recent years, many attempts have been made to supply uncomplicated, rapid, economical and user-friendly analytical approaches for the on-site detection and antioxidant capacity (AOC) determination of food antioxidants. In this regards, sensors and biosensors are regarded as favorable tools for antioxidant analysis because of their special features like high sensitivity, rapid detection time, ease of use, and ease of miniaturization. In this review, current five-year progresses in different types of optical and electrochemical sensors/biosensors for the analysis of antioxidants in foods are discussed and evaluated well. Moreover, advantages, limitations, and the potential for practical applications of each type of sensors/biosensors have been discussed. This review aims to prove how sensors/biosensors represent reliable alternatives to conventional methods for antioxidant analysis.
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http://dx.doi.org/10.3390/s21041176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7915219PMC
February 2021

Wearable Biosensors: An Alternative and Practical Approach in Healthcare and Disease Monitoring.

Molecules 2021 Feb 1;26(3). Epub 2021 Feb 1.

University of Perpignan via Domitia, 52 Avenue Paul Alduy, CEDEX 9, 66860 Perpignan, France.

With the increasing prevalence of growing population, aging and chronic diseases continuously rising healthcare costs, the healthcare system is undergoing a vital transformation from the traditional hospital-centered system to an individual-centered system. Since the 20th century, wearable sensors are becoming widespread in healthcare and biomedical monitoring systems, empowering continuous measurement of critical biomarkers for monitoring of the diseased condition and health, medical diagnostics and evaluation in biological fluids like saliva, blood, and sweat. Over the past few decades, the developments have been focused on electrochemical and optical biosensors, along with advances with the non-invasive monitoring of biomarkers, bacteria and hormones, etc. Wearable devices have evolved gradually with a mix of multiplexed biosensing, microfluidic sampling and transport systems integrated with flexible materials and body attachments for improved wearability and simplicity. These wearables hold promise and are capable of a higher understanding of the correlations between analyte concentrations within the blood or non-invasive biofluids and feedback to the patient, which is significantly important in timely diagnosis, treatment, and control of medical conditions. However, cohort validation studies and performance evaluation of wearable biosensors are needed to underpin their clinical acceptance. In the present review, we discuss the importance, features, types of wearables, challenges and applications of wearable devices for biological fluids for the prevention of diseased conditions and real-time monitoring of human health. Herein, we summarize the various wearable devices that are developed for healthcare monitoring and their future potential has been discussed in detail.
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http://dx.doi.org/10.3390/molecules26030748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867046PMC
February 2021

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring.

Toxins (Basel) 2020 Dec 24;13(1). Epub 2020 Dec 24.

BAE-LBBM Laboratory, University of Perpignan via Domitia, 52 Avenue Paul Alduy, CEDEX 9, 66860 Perpignan, France.

Mycotoxins contamination is a global public health concern. Therefore, highly sensitive and selective techniques are needed for their on-site monitoring. Several approaches are conceivable for mycotoxins analysis, among which colorimetric methods are the most attractive for commercialization purposes thanks to their visual read-out, easy operation, cost-effectiveness, and rapid response. This review covers the latest achievements in the last five years for the development of colorimetric methods specific to mycotoxins analysis, with a particular emphasis on their potential for large-scale applications in food industries. Gathering all types of (bio)receptors, main colorimetric methods are critically discussed, including enzyme-linked assays, lateral flow-assays, microfluidic devices, and homogenous in-solution strategies. This special focus on colorimetry as a versatile transduction method for mycotoxins analysis is comprehensively reviewed for the first time.
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http://dx.doi.org/10.3390/toxins13010013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823678PMC
December 2020

Investigation of a Truncated Aptamer for Ofloxacin Detection Using a Rapid FRET-Based Apta-Assay.

Antibiotics (Basel) 2020 Dec 3;9(12). Epub 2020 Dec 3.

Laboratoire BAE-LBBM, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, CEDEX 9, 66860 Perpignan, France.

In this work, we describe the use of a new truncated aptamer for the determination of ofloxacin (OFL), being a principal quinolone commonly used in both human and animal healthcare. Since the affinity of a 72-mer ssDNA sequence has been previously described without further investigations, this paper demonstrates the first computational prediction of the binding motif between this aptamer and OFL through in silico molecular docking studies. Besides, we suggest the application of the characterized recognition mechanism in a simple FRET (Förster Resonance Energy Transfer) pattern for the rapid aptasensing of the quinolone of interest. Accordingly, our approach harnesses the fluorescence quenching of the fluorescein-tagged aptamer (FAM-APT) induced by its partial hybridization to a tetramethyl rhodamine-labelled complementary ssDNA (TAMRA-cDNA). In such a structure, dye labels brought into close proximity act as a FRET pair. Upon ofloxacin addition, an affinity competition occurs to form a more stable FAM-APT/OFL complex, thus unquenching the FAM-APT signal. Interestingly, the recovered fluorescence intensity was found to correlate well with the antibiotic's concentrations in the range of 0.2-200 μM in HEPES buffer, with a linear response that ranged between 0.2 and 20 μM. The rapid apta-assay achieved limits of detection and quantification of 0.12 and 0.40 μM, respectively. The truncated aptamer has also shown an improved specificity toward OFL than other quinolones, compared to the original full-length aptamer described in previous works. Finally, the practical application of the developed apta-assay was successfully confirmed to detect OFL quinolone in spiked milk samples, with satisfactory recoveries ranging between 97.4% and 111.4%.
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http://dx.doi.org/10.3390/antibiotics9120860DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7761777PMC
December 2020

Urea Biosensor Based on a CO Microsensor.

ACS Omega 2020 Oct 19;5(42):27582-27590. Epub 2020 Oct 19.

Aarhus University Centre for Water Technology (WATEC), Department of Biology, Aarhus University, Ny Munkegade 114-116, Aarhus C 8000, Denmark.

Urea sensors based on electrodes in direct contact with the medium have limited long-term stability when exposed to complex media. Here, we present a urea biosensor based on urease immobilized in an alginate polymer, buffered at pH 6, and placed in front of a newly developed fast and sensitive CO microsensor, where the electrodes are shielded by a gas-permeable membrane. The CO produced by the urease in the presence of urea diffuses into the microsensor and is reduced at a Ag cathode. Oxygen interference is prevented by a Cr trap. The 95% response time to changes in urea concentration was 120 s with a linear calibration curve in the range 0-1000 μM and a detection limit of 1 μM. The Ni cofactor to improve sensor performance was continuously supplied from a reservoir behind the sensor tip. The stability of the urea sensor was optimized by the addition of bovine serum albumin as a stabilizer to the urease/alginate mixture that was cross-linked with glutaraldehyde and Ca ions. This immobilization strategy resulted in about 70% of the initial urea sensor sensitivity after two weeks of continuous operation. The sensor was successfully tested in blood serum.
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http://dx.doi.org/10.1021/acsomega.0c04146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7594316PMC
October 2020

Development of a label-free electrochemical aptasensor based on diazonium electrodeposition: Application to cadmium detection in water.

Anal Biochem 2021 01 17;612:113956. Epub 2020 Sep 17.

Laboratory of Biosensors, Analysis and Environment (BAE), University of Perpignan Via Domitia, Perpignan, France; Higher National School of Biotechnology, Constantine, Algeria. Electronic address:

In this study we have developed a new aptasensor for cadmium (Cd) detection in water. Gold electrode surface has been chemically modified by electrochemical reduction of diazonium salt (CMA) with carboxylic acid outward from the surface. This was used for amino-modified cadmium aptamer immobilization through carbodiimide reaction. Chemical surface modification was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This latter was also used for Cd detection. The aptasensor has exhibited a good linear relationship between the logarithm of the Cd concentration and the impedance changes in the range from 10 to 10 M with a correlation R of 0.9954. A high sensitivity was obtained with a low limit of detection (LOD) of 2.75*10 M. Moreover, the developed aptasensor showed a high selectivity towards Cd when compared to other interferences such as Hg, Pb and Zn. The developed aptasensor presents a simple and sensitive approach for Cddetection in aqueous solutions with application for trace Cd detection in spring water samples.
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http://dx.doi.org/10.1016/j.ab.2020.113956DOI Listing
January 2021

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2): a global pandemic and treatment strategies.

Int J Antimicrob Agents 2020 Aug 10;56(2):106054. Epub 2020 Jun 10.

Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan CEDEX 66860, France; Sensbiotech, 21rue de Nogarede, 66400 Ceret, France.

The emergence and rapid spread of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), a potentially fatal disease, is swiftly leading to public health crises worldwide. The origin of SARS-CoV-2 infection was first reported in people exposed to a seafood market in Wuhan City, China in December 2019. It has been suggested that the infection is likely to be of zoonotic origin and transmitted to humans through a not-yet-known intermediary. As of 22 May 2020, the World Health Organization reported that there were approximately 4,995,996 confirmed cases and 327,821 deaths. SARS-CoV-2 is transmitted via inhalation or direct contact with droplets from infected people. It has an incubation period ranging from 2 to ≥14 days. The rate of spread of SARS-CoV-2 is greater than that for severe acute respiratory syndrome coronavirus and Middle East respiratory coronavirus. The symptoms are similar to influenza (i.e. breathlessness, sore throat and fatigue) and infected cases are isolated and treated. Infection is mild in most cases, but in elderly (>50 years) patients and those with cardiac and respiratory disorders, it may progress to pneumonia, acute respiratory distress syndrome and multi-organ failure. People with strong immunity or those who have developed herd immunity are asymptomatic. The fatality rate ranges from 3% to 4%. Recommended methods for diagnosis of COVID-19 are molecular tests (e.g. polymerase chain reaction) on respiratory secretions, chest scan and common laboratory diagnosis. Currently, treatment is essentially supportive, and the role of antiviral agents is yet to be established as a vaccine is not yet available. This review will focus on epidemiology, symptoms, transmission, pathogenesis, ongoing available treatments and future perspectives of SARS-CoV-2.
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http://dx.doi.org/10.1016/j.ijantimicag.2020.106054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286265PMC
August 2020

Immobilization of Enzymes on Magnetic Beads Through Affinity Interactions.

Methods Mol Biol 2020 ;2100:189-198

BAE, Universite de Perpignan Via Domitia, Ceret, France.

The development of enzyme immobilization techniques that will not affect catalytic activity and conformation is an important research task. Affinity tags that are present or added at a specific position far from the active site in the structure of the native enzyme could be used to create strong affinity bonds between the protein structure and a surface functionalized with the complementary affinity ligand. These immobilization techniques are based on affinity interactions between biotin and (strept)avidin molecules, lectins and sugars, or metal chelate and histidine tag.Recent developments involve immobilization of tagged enzymes onto magnetic nanoparticles. These supports can improve the performance of immobilized biomolecules in analytical assay because magnetic beads provide a relative large numbers of binding sites for biochemical reactions resulting in faster assay kinetics.This chapter describes immobilization procedures of tagged enzymes onto various magnetic beads.
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http://dx.doi.org/10.1007/978-1-0716-0215-7_12DOI Listing
January 2021

Switchable fluorescence sensor toward PAT via CA-MWCNTs quenched aptamer-tagged carboxyfluorescein.

Food Chem 2020 May 17;312:126048. Epub 2019 Dec 17.

Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS University Islamabad, Lahore Campus, 54000, Pakistan. Electronic address:

A quenching based apta-sensing platform was developed for the detection of Patulin. Three different aptamer sequences were studied to screen the aptamer with the maximum affinity towards Patulin. Carboxyfluorescein (CFL) was used as a fluorescent dye while -COOH functionalized multiwall carbon nanotubes (MWCNTs) were applied as novel nanoquenchers. Aptamer tagged at the 3' end with 40 nucleotide bases exhibited the maximum affinity towards Patulin and caused substantial fluorescence recovery. Interestingly, the limit of detection (LOD) and limit of quantification (LOQ) were calculated as 0.13 μg Land 0.41 μg L respectively. Commonly occurring mycotoxins in food were also tested to confirm the selectivity of apta-assay. The developed apta-assay was applied to a spiked apple juice sample and toxin recoveries were observed ranging from 96% to 98% (n = 3). These results demonstrated the potential of the developed apta-assay for the selective detection and quantification of Patulin in food samples.
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http://dx.doi.org/10.1016/j.foodchem.2019.126048DOI Listing
May 2020

Polymer scaffold layers of screen-printed electrodes for homogeneous deposition of silver nanoparticles: application to the amperometric detection of hydrogen peroxide.

Mikrochim Acta 2019 11 19;186(12):810. Epub 2019 Nov 19.

BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860, Perpignan, France.

A method is described for electrochemical oxidation of polymers on the surface of screen-printed electrodes (SPCE). These act as scaffold layers for homogeneous deposition of silver nanoparticles (AgNPs). Hexamethylenediamine (HMDA) and poly(ethylene glycol) were immobilized on the SPCE surface via electrochemical oxidation. AgNPs were then electrodeposited on the scaffolds on the SPCE. This type of different carbon chain containing materials like PEG and HMDA act as big tunnels for electron mobility and are useful for the homogenous deposition of AgNPs on the SPCE surface without agglomeration. The resulting sensor was applied to the determination of hydrogen peroxide (HO) as a model analyte. It is found to display favorable catalytic and conductive properties towards the reduction of HO. Cyclic voltammetry and amperometry revealed that the modified electrode performs better than other modified SPCEs. Best operated at a potential of around -0.61 V (vs Ag|AgCl), the amperometric response is linear in the 10-180 μM HO concentration range and the detection limit is 1.5 μM. The sensor is stable and reproducible. The resultant sensor was appplied to toothpaste analysis, and good recovery values were gained. Graphical abstractSchematic representation of electropolymerization of poly(ethylene glycol) and hexamethylenediamine scaffold layers on screen-printed electrodes for homogeneous electrodeposition of silver nanoparticles. This electrode was applied for the amperometric determination of hydrogen peroxide.
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http://dx.doi.org/10.1007/s00604-019-3963-yDOI Listing
November 2019

Highly sensitive label-free in vitro detection of aflatoxin B1 in an aptamer assay using optical planar waveguide operating as a polarization interferometer.

Anal Bioanal Chem 2019 Nov 7;411(29):7717-7724. Epub 2019 Aug 7.

Agro-Environmental Research Institute, NARIC, Budapest, 2100, Hungary.

This work reports on further development of an optical biosensor for the in vitro detection of mycotoxins (in particular, aflatoxin B1) using a highly sensitive planar waveguide transducer in combination with a highly specific aptamer bioreceptor. This sensor is built on a SiO-SiN-SiO optical planar waveguide (OPW) operating as a polarization interferometer (PI), which detects a phase shift between p- and s-components of polarized light propagating through the waveguide caused by the molecular adsorption. The refractive index sensitivity (RIS) of the recently upgraded PI experimental setup has been improved and reached values of around 9600 rad per refractive index unity (RIU), the highest RIS values reported, which enables the detection of low molecular weight analytes such as mycotoxins in very low concentrations. The biosensing tests yielded remarkable results for the detection of aflatoxin B1 in a wide range of concentrations from 1 pg/mL to 1 μg/mL in direct assay with specific DNA-based aptamers. Graphical abstract Optical planar waveguide polarization interferometry biosensor for detection of aflatoxin B1 using specific aptamer.
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http://dx.doi.org/10.1007/s00216-019-02033-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881424PMC
November 2019

Development of a highly sensitive xanthine oxidase-based biosensor for the determination of antioxidant capacity in Amazonian fruit samples.

Talanta 2019 Nov 4;204:626-632. Epub 2019 Jun 4.

BAE-LBBM, University of Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860, Perpignan, Cedex, France. Electronic address:

The paper describes the development of an amperometric biosensor using Prussian Blue (PB) modified electrodes containing xanthine oxidase (XOD). The enzyme is immobilized by photo-polymerization into an azide-unit pendant water-soluble photopolymer (PVA-AWP). The parameters of the fabrication of the biosensor, XOD:PVA/AWP ratio, crosslinking irradiation time, and XOD charge, were optimized. Operational conditions for electrode preparation were defined as 1:2 ratio of XOD:PVA/AWP; exposure time to neon light of 30  min; pH = 7.5  at room temperature and enzymatic charge of 8 mU per electrode. The biosensors showed stable, fast, simple, selective, cost-effective and sensitive (-2.72E-8  A mol L), with a good linear range (1.0-75  μmol L), and respectively detection and quantification limits for antioxidants of 2.17, and 7.15  μmol L. The applicability of this biosensor was demonstrated by in vitro analysis of gallic acid as standard antioxidant and Amazonian fruits as natural sources.
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http://dx.doi.org/10.1016/j.talanta.2019.06.002DOI Listing
November 2019

Ultrasensitive ciprofloxacin assay based on the use of a fluorescently labeled aptamer and a nanocomposite prepared from carbon nanotubes and MoSe.

Mikrochim Acta 2019 07 3;186(8):507. Epub 2019 Jul 3.

School of Environmental and Materials Engineering, College of Engineering, Shanghai Polytechnic University, Shanghai, 201209, China.

A nanocomposite was prepared from carbon nanotubes and MoSe (CNT-MoSe). This nanomaterial quenches the fluorescence of fluorescein-labeled aptamers. When ciprofloxacin (CIP) binds to the aptamer, an aptamer/G-quadruplex complex will be formed and the interaction between labeled aptamer and CNT-MoSe nanostructures is weakened. This leads to significant fluorescence recovery. Under optimized experimental conditions, the limit of detection is 0.63 ng mL with a good linearity in the range from 0.63 to 80 ng mL. The assay was applied to the determination of CIP in spiked milk, and the recoveries range between 94.3 and 97.0% (n = 3). Conceivably, the method is a generic approach that can be extended to the determination of other analyte for which adequate aptamers are available. Graphical abstract Schematic presentation of CNT-MoSe quenching based aptamer assay for the detection of ciprofloxacin. The assay exhibits good selectivity, stability and reproducibility, and low limit of detection.
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http://dx.doi.org/10.1007/s00604-019-3629-9DOI Listing
July 2019

A Review of the Construction of Nano-Hybrids for Electrochemical Biosensing of Glucose.

Biosensors (Basel) 2019 Mar 25;9(1). Epub 2019 Mar 25.

BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan ViaDomitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, France.

Continuous progress in the domain of nano and material science has led to modulation of the properties of nanomaterials in a controlled and desired fashion. In this sense, nanomaterials, including carbon-based materials, metals and metal oxides, and composite/hybrid materials have attracted extensive interest with regard to the construction of electrochemical biosensors. The modification of a working electrode with a combination of two or three nanomaterials in the form of nano-composite/nano-hybrids has revealed good results with very good reproducibility, stability, and improved sensitivity. This review paper is focused on discussing the possible constructs of nano-hybrids and their subsequent use in the construction of electrochemical glucose biosensors.
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http://dx.doi.org/10.3390/bios9010046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468850PMC
March 2019

Development of an Impedimetric Aptasensor for Label Free Detection of Patulin in Apple Juice.

Molecules 2019 Mar 13;24(6). Epub 2019 Mar 13.

BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, France.

In the present work, an aptasensing platform was developed for the detection of a carcinogenic mycotoxin termed patulin (PAT) using a label-free approach. The detection was mainly based on a specific interaction of an aptamer immobilized on carbon-based electrode. A long linear spacer of carboxy-amine polyethylene glycol chain (PEG) was chemically grafted on screen-printed carbon electrodes (SPCEs) via diazonium salt in the aptasensor design. The NH₂-modified aptamer was then attached covalently to carboxylic acid groups of previously immobilized bifunctional PEG to build a diblock macromolecule. The immobilized diblocked molecules resulted in the formation of long tunnels on a carbon interface, while the aptamer was assumed as the gate of these tunnels. Upon target analyte binding, the gates were assumed to be closed due to conformational changes in the structure of the aptamer, increasing the resistance to the charge transfer. This increase in resistance was measured by electrochemical impedance spectroscopy, the main analytical technique for the quantitative detection of PAT. Encouragingly, a good linear range between 1 and 25 ng was obtained. The limit of detection and limit of quantification was 2.8 ng L and 4.0 ng L, respectively. Selectivity of the aptasensor was confirmed with mycotoxins commonly occurring in food. The developed apta-assay was also applied to a real sample, i.e., fresh apple juice spiked with PAT, and toxin recovery up to 99% was observed. The results obtained validated the suitability and selectivity of the developed apta-assay for the identification and quantification of PAT in real food samples.
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http://dx.doi.org/10.3390/molecules24061017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6471267PMC
March 2019

Optical and Electrochemical Sensors and Biosensors for the Detection of Quinolones.

Trends Biotechnol 2019 08 15;37(8):898-915. Epub 2019 Feb 15.

Biocapteurs-Analyses-Environnement (BAE), Universite de Perpignan Via Domitia, Perpignan Cedex 66860, France. Electronic address:

One major concern associated with food safety is related to residual effects of antibiotics that are widely used to treat animals and result in antimicrobial resistance. Among different groups of antibiotic, the use of quinolones in livestock is of major concern due to the significance of these antimicrobial drugs for the treatment of a range of infectious diseases in humans. Therefore, it is desirable to develop reliable methods for the rapid, sensitive, and on-site detection of quinolone residue levels in animal-derived foods to ensure food safety. Sensors and biosensors are promising future platforms for rapid and on-site monitoring of antibiotic residues. In this review, we focus on recent advancements and modern approaches in quinolone sensors and biosensors.
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http://dx.doi.org/10.1016/j.tibtech.2019.01.004DOI Listing
August 2019

Design of a redox-active surface for ultrasensitive redox capacitive aptasensing of aflatoxin M1 in milk.

Talanta 2019 Apr 14;195:525-532. Epub 2018 Nov 14.

Université de Tunis El Manar, Faculté des Sciences de Tunis, Laboratoire de Chimie Analytique et Electrochimie (LR99ES15), Sensors and Biosensors Group, Campus Universitaire de Tunis El Manar, 2092 Tunis, Tunisia. Electronic address:

Herein, we report the design of a novel label-free aptasensor based on ferrocene and silicon nanoparticles (SiNPs) for ultrasensitive detection of aflatoxin M1 (AFM1) in milk. Given that silicon nanomaterials stand out by their high capacitive power, we used them to develop a novel capacitive transduction system based on electrochemical capacitance spectroscopy (ECS). This strategy relies on the changes of the redox capacitance signal owed to the surface-tethered ferrocene film, by performing electrochemical impedance spectroscopy (EIS) measurements without using an external redox probe. The redox capacitance variation was found to correlate well with the increasing concentrations of AFM1 in the linear range from 10 to 500 fmol⋅L with a sensitivity of 0.46 μF*fM-1*cm - 2. Furthermore, the aptasensor allowed to reach very low limits of detection and quantification equal to 4.53 fM and 14.95 fM, respectively. The platform revealed a high selectivity toward the target analyte, and it was applied to quantify very low concentrations of AFM1 in commercial pasteurized milk. Finally, the results of real sample analysis were successfully gauged against those obtained using commercially available enzyme-linked immunoassay (ELISA) kits.
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http://dx.doi.org/10.1016/j.talanta.2018.11.026DOI Listing
April 2019

Label-Free Optical Detection of Mycotoxins Using Specific Aptamers Immobilized on Gold Nanostructures.

Toxins (Basel) 2018 07 16;10(7). Epub 2018 Jul 16.

Agro-Environmental Research Institute, NARIC, 1011-1239 Budapest, Hungary.

This work focuses on the development of the novel label-free optical apta-sensors for detection of mycotoxins. A highly sensitive analytical method of total internal reflection ellipsometry (TIRE) combined with Localized Surface Plasmon Resonance (LSPR) phenomenon in nano-structured gold films was exploited here for the first time for detection of aflatoxin B1 and M1 in direct assay with specific aptamers immobilized on the surface of gold. The achieved detection of low molecular weight molecules, such as aflatoxin B1 and M1, in a wide range of concentrations from 100 ng/mL down to 0.01 ng/mL is remarkable for the LSPR method. The study of binding kinetics of aflatoxin molecules to their respective aptamers using dynamic TIRE measurements yielded the values of affinity constants in the range of 10⁻10 mol, which is characteristic for highly specific aptamer/target interactions similar to that for monoclonal antibodies. The effect of aptamers' DNA chain length on their binding characteristics was analyzed.
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http://dx.doi.org/10.3390/toxins10070291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6071250PMC
July 2018

Development of a portable and disposable NS1 based electrochemical immunosensor for early diagnosis of dengue virus.

Anal Chim Acta 2018 Oct 18;1026:1-7. Epub 2018 Apr 18.

BAE: Biocapteurs-Analyses-Environment, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan Cedex, 66860, France. Electronic address:

The present study represents fabrication of nonstructural antibody (NS1) based immunosensor coupled with bovine serum albumin (BSA) modified screen printed carbon electrodes (SPCE) as transducing substrate for the early diagnosis of dengue virus. The anti-NS1 monoclonal antibody was immobilized on electro grafted BSA surface of working electrode. The electrons transfer resistance before and after NS1 attachment was monitored as a function of its concentration to perform the qualitative and quantitative analysis. The as prepared impedimetric immunosensor successfully detected the dengue virus protein with enhanced limit of detection (0.3 ng/mL) and linear range (1-200 ng/mL). The selectivity of the designed device was further elaborated with several interfering analytes and was finally demonstrated with human serum samples. The extravagant selectivity, sensitivity and easier fabrication protocol corroborate the potential applications of such immunosensor for practical diagnosis of dengue virus.
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http://dx.doi.org/10.1016/j.aca.2018.04.032DOI Listing
October 2018

Label free aptasensor for ochratoxin A detection using polythiophene-3-carboxylic acid.

Talanta 2018 Aug 29;185:513-519. Epub 2018 Mar 29.

BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France.

This work demonstrates the development of electrochemical aptasensor using ochratoxin A (OTA) aptamers. Different aptamer coupling strategies were tested using polythiophene-carboxylic acid (PT3C) and polypyrrole-3-carboxylic acid (PP3C). The best sensitivity was recorded by polythiophene-3-carboxylic acid (PT3C) on screen-printed carbon electrode (SPCE) to attain the direct detection of OTA. The quantification of OTA was achieved by using electrochemical impedance spectroscopy. A good dynamic range 0.125-2.5 ng ml was obtained for OTA with limit of detection (LOD) 0.125 ng ml and Limit of quantification (LOQ) 0.3 ng ml respectively. The good reproducibility was recorded with RSD% of 3.68. The obtained straight line equation was y = 0.4061 × + 1.03, r = 0.99. For real sample applications, the developed aptasensors were demonstrated in coffee samples. The aptasensor displayed good recovery values in the range 88-89%, thus exhibited the effectiveness of proposed aptasensor for such complex matrices.
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http://dx.doi.org/10.1016/j.talanta.2018.03.089DOI Listing
August 2018

Designed Strategies for Fluorescence-Based Biosensors for the Detection of Mycotoxins.

Toxins (Basel) 2018 05 11;10(5). Epub 2018 May 11.

BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, France.

Small molecule toxins such as mycotoxins with low molecular weight are the most widely studied biological toxins. These biological toxins are responsible for food poisoning and have the potential to be used as biological warfare agents at the toxic dose. Due to the poisonous nature of mycotoxins, effective analysis techniques for quantifying their toxicity are indispensable. In this context, biosensors have been emerged as a powerful tool to monitors toxins at extremely low level. Recently, biosensors based on fluorescence detection have attained special interest with the incorporation of nanomaterials. This review paper will focus on the development of fluorescence-based biosensors for mycotoxin detection, with particular emphasis on their design as well as properties such as sensitivity and specificity. A number of these fluorescent biosensors have shown promising results in food samples for the detection of mycotoxins, suggesting their future potential for food applications.
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http://dx.doi.org/10.3390/toxins10050197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983253PMC
May 2018

Photoinduced discharge of electrons stored in a TiO2-MWCNT composite to an analyte: application to the fluorometric determination of hydrogen peroxide, glucose and aflatoxin B1.

Mikrochim Acta 2017 12 6;185(1):26. Epub 2017 Dec 6.

Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology (CIIT), Lahore, 54000, Pakistan.

The authors describe an analytical detection scheme based on the use of multiwalled carbon nanotubes (MWCNTs) that accept and store electrons upon contact with photo-irradiated TiO nanoparticles (TiO-NPs). The Fermi level equilibration with photo-irradiated TiO-NPs has a storage value of 0.35 mM of electrons per 120 mg·L of MWCNTs. The stored electrons can be discharged on demand upon addition of electron acceptors to the TiO-NP/MWCNT composite. These findings are applied to detect the quencher hydrogen peroxide. HO also is produced on enzymatic action of glucose oxidase on glucose, and this enables glucose also to be quantified by using the TiO-NP/MWCNT fluorescent nanoprobe. The wide scope of the method also is demonstrated by an assay for aflatoxin B1 that is making use of an FAM-labeled aptamer where the FAM fluorophore on the aptamer quenches the emission of the nanoprobe. The following analytical linear ranges and limits of detection are found: HO: 0.1-100 μM and 15 nM; glucose: 5-200 μM and 0.5 μM; aflatoxin: 0.1-40 ng·mL and 0.02 ng·mL. The method was applied to the determination of glucose in human serum. Graphical abstract The assays demonstrated in (b) and (c) are based on the fluorescence quenching ability of MWCNTs-TiO. In the presence of the target (analyte), the fluorescence is restored and the target concentration is determined from the percentage of fluorescence recovery.
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http://dx.doi.org/10.1007/s00604-017-2583-7DOI Listing
December 2017

Advances in Enzyme-Based Biosensors for Pesticide Detection.

Biosensors (Basel) 2018 Mar 22;8(2). Epub 2018 Mar 22.

International Centre of Biodynamics, 1B Intrarea Portocalelor, 060101 Bucharest, Romania.

The intensive use of toxic and remanent pesticides in agriculture has prompted research into novel performant, yet cost-effective and fast analytical tools to control the pesticide residue levels in the environment and food. In this context, biosensors based on enzyme inhibition have been proposed as adequate analytical devices with the added advantage of using the toxicity of pesticides for detection purposes, being more "biologically relevant" than standard chromatographic methods. This review proposes an overview of recent advances in the development of biosensors exploiting the inhibition of cholinesterases, photosynthetic system II, alkaline phosphatase, cytochrome P450A1, peroxidase, tyrosinase, laccase, urease, and aldehyde dehydrogenase. While various strategies have been employed to detect pesticides from different classes (organophosphates, carbamates, dithiocarbamates, triazines, phenylureas, diazines, or phenols), the number of practical applications and the variety of environmental and food samples tested remains limited. Recent advances focus on enhancing the sensitivity and selectivity by using nanomaterials in the sensor assembly and novel mutant enzymes in array-type sensor formats in combination with chemometric methods for data analysis. The progress in the development of solar cells enriched the possibilities for efficient wiring of photosynthetic enzymes on different surfaces, opening new avenues for development of biosensors for photosynthesis-inhibiting herbicides.
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http://dx.doi.org/10.3390/bios8020027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022933PMC
March 2018

Detection of Antibiotics and Evaluation of Antibacterial Activity with Screen-Printed Electrodes.

Sensors (Basel) 2018 Mar 18;18(3). Epub 2018 Mar 18.

International Centre of Biodynamics, 1B Intrarea Portocalelor, Bucharest 060101, Romania.

This review provides a brief overview of the fabrication and properties of screen-printed electrodes and details the different opportunities to apply them for the detection of antibiotics, detection of bacteria and antibiotic susceptibility. Among the alternative approaches to costly chromatographic or ELISA methods for antibiotics detection and to lengthy culture methods for bacteria detection, electrochemical biosensors based on screen-printed electrodes present some distinctive advantages. Chemical and (bio)sensors for the detection of antibiotics and assays coupling detection with screen-printed electrodes with immunomagnetic separation are described. With regards to detection of bacteria, the emphasis is placed on applications targeting viable bacterial cells. While the electrochemical sensors and biosensors face many challenges before replacing standard analysis methods, the potential of screen-printed electrodes is increasingly exploited and more applications are anticipated to advance towards commercial analytical tools.
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http://dx.doi.org/10.3390/s18030901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877114PMC
March 2018

Carboxylic group riched graphene oxide based disposable electrochemical immunosensor for cancer biomarker detection.

Anal Biochem 2018 03 12;545:13-19. Epub 2018 Jan 12.

Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology (CIIT), Lahore, 54000, Pakistan. Electronic address:

In this work, we have developed for the first time a carboxylic group riched graphene oxide based disposable electrochemical immunosensor for cancer biomarker detection using methylene blue (MB). The developed immunosensor is highly sensitive for detection of biomarker Mucin1 (MUC1) in human serum samples. Development of this disposable electrochemical immunosensor was premeditated by applying specific monoclonal antibodies against MUC1. In this method, we explored highly conductive surface of carboxylic group (-COOH-) rich graphene oxide (GO) on screen-printed carbon electrodes (SPCE). This modified GO-COOH-SPCE was employed for the detection of MUC1 protein based on the reaction with methylene blue (MB) redox probe using differential pulse voltammetry (DPV) technique. Developed immunosensor exhibited good detection range for MUC1 with excellent linearity (0.1 U/mL- 2 U/mL), with a limit of detection of 0.04 U/mL. Upon potential application of developed biosensor, good recoveries were recorded in the range of 96-96.67% with % R.S.D 4.2. Analytical performance of the developed immunosensor assures the applicability in clinical diagnostic applications.
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http://dx.doi.org/10.1016/j.ab.2018.01.007DOI Listing
March 2018

Nano-Aptasensing in Mycotoxin Analysis: Recent Updates and Progress.

Toxins (Basel) 2017 10 28;9(11). Epub 2017 Oct 28.

BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860 Perpignan CEDEX, France.

Recent years have witnessed an overwhelming integration of nanomaterials in the fabrication of biosensors. Nanomaterials have been incorporated with the objective to achieve better analytical figures of merit in terms of limit of detection, linear range, assays stability, low production cost, etc. Nanomaterials can act as immobilization support, signal amplifier, mediator and artificial enzyme label in the construction of aptasensors. We aim in this work to review the recent progress in mycotoxin analysis. This review emphasizes on the function of the different nanomaterials in aptasensors architecture. We subsequently relate their features to the analytical performance of the given aptasensor towards mycotoxins monitoring. In the same context, a critically analysis and level of success for each nano-aptasensing design will be discussed. Finally, current challenges in nano-aptasensing design for mycotoxin analysis will be highlighted.
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http://dx.doi.org/10.3390/toxins9110349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5705964PMC
October 2017

An Overview on Recent Progress in Electrochemical Biosensors for Antimicrobial Drug Residues in Animal-Derived Food.

Sensors (Basel) 2017 Aug 24;17(9). Epub 2017 Aug 24.

BAE: Biocapteurs-Analyses-Environnement, Universite de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan CEDEX 66860, France.

Anti-microbial drugs are widely employed for the treatment and cure of diseases in animals, promotion of animal growth, and feed efficiency. However, the scientific literature has indicated the possible presence of antimicrobial drug residues in animal-derived food, making it one of the key public concerns for food safety. Therefore, it is highly desirable to design fast and accurate methodologies to monitor antimicrobial drug residues in animal-derived food. Legislation is in place in many countries to ensure antimicrobial drug residue quantities are less than the maximum residue limits (MRL) defined on the basis of food safety. In this context, the recent years have witnessed a special interest in the field of electrochemical biosensors for food safety, based on their unique analytical features. This review article is focused on the recent progress in the domain of electrochemical biosensors to monitor antimicrobial drug residues in animal-derived food.
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http://dx.doi.org/10.3390/s17091947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5621119PMC
August 2017

A novel colorimetric competitive aptamer assay for lysozyme detection based on superparamagnetic nanobeads.

Talanta 2017 Apr 30;165:436-441. Epub 2016 Dec 30.

Université de Perpignan via Domitia, Laboratoire BAE, Building S 52 Av. Paul Alduy, 66860 Perpignan Cedex, France. Electronic address:

Lysozyme (Lys) commonly presents in wines and are known to cause toxicological impact on human health. The need of highly sensitive and reliable detection methods are evident in such matrix. In this work, we developed a competitive aptamer based assay for detection of Lys by employing carboxylated magnetic beads as a support to immobilize the target molecule Lys. The used aptamer sequence was biotinylated which further binds with Streptavidin-Alkaline phosphatase (Stp-ALP) in the micro wells. Colorimetric tests were performed in order to optimize different experimental parameters. The Lys assay showed a good linearity in the range of 5-140nM with a limit of detection (LOD) 10nM. The mid-point value (IC) 110nM and the analysis time (60min) validated the developed aptasensor as a promising tool for routine use. The assay displayed good recoveries of Lys in the range 99.00-99.27% and was demonstrated for the detection of Lys in wine samples.
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http://dx.doi.org/10.1016/j.talanta.2016.12.083DOI Listing
April 2017

Label-Free Aptasensors for the Detection of Mycotoxins.

Sensors (Basel) 2016 Dec 18;16(12). Epub 2016 Dec 18.

BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France.

Various methodologies have been reported in the literature for the qualitative and quantitative monitoring of mycotoxins in food and feed samples. Based on their enhanced specificity, selectivity and versatility, bio-affinity assays have inspired many researchers to develop sensors by exploring bio-recognition phenomena. However, a significant problem in the fabrication of these devices is that most of the biomolecules do not generate an easily measurable signal upon binding to the target analytes, and signal-generating labels are required to perform the measurements. In this context, aptamers have been emerged as a potential and attractive bio-recognition element to design label-free aptasensors for various target analytes. Contrary to other bioreceptor-based approaches, the aptamer-based assays rely on antigen binding-induced conformational changes or oligomerization states rather than binding-assisted changes in adsorbed mass or charge. This review will focus on current designs in label-free conformational switchable design strategies, with a particular focus on applications in the detection of mycotoxins.
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http://dx.doi.org/10.3390/s16122178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5191157PMC
December 2016