Publications by authors named "Javier Mena"

9 Publications

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Hydroethanolic Extracts of the Aristotelia Chilensis (Maqui) Berry Reduces Cellular Viability and Invasiveness in the Endometrial Cancer Cell Line Ishikawa.

Integr Cancer Ther 2021 Jan-Dec;20:15347354211007560

Laboratorio de Inmunología de la Reproducción, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.

Cancer of the reproductive tract includes diseases with higher prevalence in the female population. This investigation examined whether an anthocyanin-enriched extract of , commonly known as "maqui," could affect some hallmarks of endometrial cancer. Cultures of the human endometrial cancer cell line Ishikawa were treated with a hydroethanolic maqui extract at 1, 3, 10, 30, 100, 300, or 1000 µg/mL to determine the effect on cell viability by MTT assay. Then, we used the 50% Effective Concentration (EC50) to evaluate whether the effect of the maqui extract is mediated via an arrest of the cell cycle or induction of apoptosis using flow cytometry or Annexin -FITC assays, respectively. The effects of sublethal doses of the maqui extract on migration and invasiveness of Ishikawa cells were also evaluated by the wound healing and Boyden Chamber assay, respectively. Our results show that the hydroethanolic maqui extract inhibits the cell viability with an EC50 of 472.3 µg/mL via increased apoptosis, and that reduces the invasive capacity but not migration of Ishikawa cells. These findings suggest that the hydroethanolic maqui extract has antineoplastic properties for endometrial cancer and merits further studies to corroborate its efficiency as anticancer therapy in reproductive organs.
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http://dx.doi.org/10.1177/15347354211007560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113921PMC
April 2021

Hydroethanolic Extracts of Remy and Palau Have Bactericide Activity and Inhibit the Ability of Enteritidis to Form Biofilm and Adhere to Human Intestinal Cells.

Biomed Res Int 2021 27;2021:3491831. Epub 2021 Jan 27.

Laboratorio de Inmunología de la Reproducción, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.

We analysed whether the hydroethanolic extracts from leaves of Remy (bailahuen) and Palau (cedron) inhibit the growth and ability of Enteritidis to form biofilms and to adhere to human intestinal epithelial cells. Herein, we first determined the total phenolic content and antioxidant and antibacterial activities of the extracts. Then, Enteritidis was treated with the extracts to analyse biofilm formation by scanning electronic microscopy and the violet crystal test. We also measured the efflux pump activity of Enteritidis since biofilm formation is associated with this phenomenon. Furthermore, the human intestinal cell line Caco-2 was infected with Enteritidis pretreated with the extracts, and 30 min later, the number of bacteria that adhered to the cell surface was quantified. Finally, we determined by qPCR the expression of genes associated with biofilm formation, namely, the diguanilate cyclase AdrA protein gene () and the BapA protein gene (), and genes associated with adhesion, namely, the transcriptional regulator HilA (). The phenolic content and antioxidant and bactericide activities were higher in bailahuen than in the cedron extract. Biofilm formation was inhibited by the extracts in a dose-dependent manner, while the activity of efflux pumps was decreased only with the cedron extract. Adhesion to Caco-2 cells was also inhibited without differences between doses and extracts. The extracts decreased the expression of ; with the cedron extract being the most efficient. The expression of is affected only with the cedron extract. We concluded that hydroethanolic extracts of bailahuen and cedron differentially inhibit the growth of Enteritidis and affect its the ability to form biofilms and to adhere to human intestinal epithelial cells. These results highlight the presence of molecules in bailahuen and cedron with a high potential for the control of the Enteritidis pathogenesis.
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http://dx.doi.org/10.1155/2021/3491831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857884PMC
May 2021

Adenosine triphosphate, polymyxin B and B16 cell-derived immunization induce anticancer response.

Immunotherapy 2021 Mar 5;13(4):309-326. Epub 2021 Jan 5.

Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, USACH, Alameda 3363, Santiago, Chile.

Whole dead tumor cells can be used as antigen source and the induction of protective immune response could be enhanced by damage-associated molecular patterns. We generated whole dead tumor cells called B16-immunogenic cell bodies (ICBs) from B16 melanoma cells by nutrient starvation and evaluated the antitumor effect of B16-ICBs plus ATP and polymyxin B (PMB). The subcutaneous immunization with B16-ICBs + PMB + ATP a 50% of tumor-free animals and induced a significant delay in tumor growth in a prophylactic approach. These results correlated with maturation of bone marrow-derived dendritic cells and activation of T CD8 lymphocytes . Altogether, ICB + ATP + PMB is efficient in inducing the antitumor efficacy of the whole dead tumor cells vaccine.
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http://dx.doi.org/10.2217/imt-2020-0209DOI Listing
March 2021

(Litre) Extract Promotes an Antitumor Response Against B16 Melanoma.

Front Pharmacol 2019 22;10:1201. Epub 2019 Oct 22.

Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.

Melanoma immunotherapy, specifically the autotransplant of dendritic cells charged with tumors antigens, has shown promising results in clinical trials. The positive clinical effects of this therapy have been associated to increased Th17 response and delayed-type hypersensitivity (DTH) against to tumor antigens. Some synthetic compounds, such as diphenylcyclopropenone (DPCP), are capable of triggering a DTH response in cutaneous malignancies and also to induce clinically relevant effects against melanoma. In this work, we evaluated Litre extract (LExT), a standardized extract of a Chilean stinging plant, (Litre). As Litre plant is known to induce DTH, we used a murine B16 melanoma model to compare the topical and intratumor efficacy of LExT with synthetic DTH inducers (DPCP and 2,4-dinitrochlorobenzene [DNCB]). LExt contained mainly long chain catechols and sesquiterpenes. The intratumor injection of LExT induced a significant delay in tumor growth, similarly topical treatment of an established tumor with 0.1% LExT ointment induced a growth delay and even tumor regression in 15% of treated animals. No significant changes were observed on the T-cell populations associated to LExT treatment, and neither DNCB nor DPCP were capable to induce none of the LExT-induced antitumoral effects. Interestingly, our results indicate that LExT induces an antitumor response against melanoma in a mouse model and could bring a new -and affordable- treatment for melanoma in humans.
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http://dx.doi.org/10.3389/fphar.2019.01201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817581PMC
October 2019

F-Spondin Is the Signal by Which 2-Methoxyestradiol Induces Apoptosis in the Endometrial Cancer Cell Line Ishikawa.

Int J Mol Sci 2019 Aug 7;20(16). Epub 2019 Aug 7.

Laboratorio de Inmunología de la Reproducción, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago 9160000, Chile.

The metabolite 2-methoxyestradiol (2ME) is an endogenous estrogen metabolite with potential therapeutic properties in reproductive cancers. However, the molecular mechanisms by which 2ME exerts its anticancer activity are not well elucidated. The purpose of this study was to determine the molecular signals associated with the apoptotic effects of 2ME in a human endometrial cancer cell line. Ishikawa cells were treated with non-apoptotic (0.1 µM) or apoptotic concentrations (5 µM) of 2ME, and 12 hours later mRNA levels for , , and were determined by real-time PCR. We then investigated by immunofluorescence and Western blot the expression and distribution of F-spondin, encoded by , in Ishikawa cells treated with 2ME 5 µM at 6, 12, or 24 h after treatment. The role of estrogen receptors (ER) in the effect of 2ME on the level was also investigated. Finally, we examined whether 2ME 5 µM induces cell death in Ishikawa cells pre-incubated with a neutralizing F-spondin antibody. Non-apoptotic or apoptotic concentrations of 2ME decreased and increased . However, was only increased with the 2ME apoptotic concentration. F-spondin protein was also increased at 12 and 24 h after 2ME treatment, while 2ME-induced increase was independent of ER. Neutralization of F-spondin blocked the effect of 2ME on the cell viability. These results show that F-spondin signaling is one of the components in the apoptotic effects of 2ME on Ishikawa cells and provide experimental evidence underlying the mechanism of action of this estrogen metabolite on cancer cells.
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http://dx.doi.org/10.3390/ijms20163850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6718992PMC
August 2019

CWM1: a general model to describe biokinetic processes in subsurface flow constructed wetlands.

Water Sci Technol 2009 ;59(9):1687-97

Institute for Sanitary Engineering and Water Pollution Control, University of Natural Resources and Applied Life Sciences, Vienna (BOKU), Muthgasse 18, A-1190 Vienna, Austria.

This paper presents the Constructed Wetland Model No1 (CWM1), a general model to describe biochemical transformation and degradation processes for organic matter, nitrogen and sulphur in subsurface flow constructed wetlands. The main objective of CWM1 is to predict effluent concentrations from constructed wetlands without predicting gaseous emissions. CWM1 describes aerobic, anoxic and anaerobic processes and is therefore applicable to both horizontal and vertical flow systems. 17 processes and 16 components (8 soluble and 8 particulate) are considered. CWM1 is based on the mathematical formulation as introduced by the IWA Activated Sludge Models (ASMs). It is important to note that besides the biokinetic model a number of other processes including porous media hydrodynamics, the influence of plants, the transport of particles/suspended matter to describe clogging processes, adsorption and desorption processes and physical re-aeration must be considered for the formulation of a full model for constructed wetlands.
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http://dx.doi.org/10.2166/wst.2009.131DOI Listing
July 2009

Lipoma of the supraspinatus muscle causing impingement syndrome: a case report.

J Shoulder Elbow Surg 2009 Jul-Aug;18(4):e3-5. Epub 2008 Dec 19.

Fraternidad-Muprespa (Medical Insurance Company for Labor Accidents), University of Granada, Granada, Spain.

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http://dx.doi.org/10.1016/j.jse.2008.09.017DOI Listing
August 2009

Recent developments in numerical modelling of subsurface flow constructed wetlands.

Sci Total Environ 2009 Jun 7;407(13):3931-43. Epub 2008 Sep 7.

Institute of Sanitary Engineering and Water Pollution Control, University of Natural Resources and Applied Life Sciences, Vienna (BOKU), Muthgasse 18, Vienna, Austria.

Numerical modelling of subsurface flow constructed wetlands (CWs) gained increasing interest during the last years. The main objective of the modelling work is, on the one hand, to increase the insight in dynamics and functioning of the complex CW system by using mechanistic or process based models that describe transformation and degradation processes in detail. As these mechanistic models are complex and therefore rather difficult to use there is, on the other hand, a need for simplified models for CW design. The design models should be premium to the currently used design guidelines that are mainly based on rules of thumb or simple first-order decay models. This paper presents an overview of the current developments on modelling of subsurface flow CWs based on the modelling work and model developments presented at the WETPOL 2007 symposium. Three kinds of models have been presented: simple transport and first-order decay models, complex mechanistic models, and a simplified model that has been developed for design of CWs. The models are presented and selected results are shown and discussed in relation to the available literature.
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http://dx.doi.org/10.1016/j.scitotenv.2008.07.057DOI Listing
June 2009

Cutaneous adverse reaction to furosemide treatment: new clinical findings.

Can Vet J 2006 Jun;47(6):576-8

Hospital Clinico Veterinario, Department of Animal Pathology, Veterinary Faculty of Zaragoza, C/Miguel Servet, 177. C.P. 50013, Zaragoza, Spain.

A poodle was admitted for investigation of pruritus and a lesional pattern of erythema and alopecia located in the dorsolumbar area. After differential diagnosis ruling out several processes a rare side effect to furosemide, not yet described in canine medicine was confirmed as the possible causative agent.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1461408PMC
June 2006