Publications by authors named "Javier Galapero"

6 Publications

  • Page 1 of 1

CHARACTERIZATION OF LESIONS INDUCED BY SPIROCERCA VULPIS (SPIRURIDAE: SPIROCERCIDAE) IN RED FOXES (VULPES VULPES).

J Wildl Dis 2021 Nov 17. Epub 2021 Nov 17.

Universidad de Extremadura, Facultad de Veterinaria, Departamento de Sanidad Animal, Parasitología, Avda. Universidad s/n, 10003, Cáceres, Spain.

Spirocerca lupi infection in dogs (Canis domesticus) is associated with esophageal lesions that may evolve to a neoplastic stage in the form of esophageal sarcoma. In the red fox (Vulpes vulpes) infected with the closely related Spirocerca vulpis, similar lesions may occur in the stomach, but neoplastic forms have not been reported. We characterize Spirocerca vulpis-induced lesions in the fox, using pathology and immunohistochemical (IHC) techniques. Seventy-one out of 163 Spirocerca vulpis-positive red foxes were selected and subjected to histopathological study. Lesions were classified as patchy or diffuse. Ten patchy and 10 diffuse lesion samples were studied using three IHC markers (CD68, CD3, and CD79α for macrophages, T lymphocytes, and B lymphocytes, respectively) and H&E stain for neutrophils and eosinophils. Intensity of necrosis, hemorrhages, and the presence of collagen was also analyzed. Of the S. vulpis-positive red foxes, 96.9% had S. vulpis nodules localized in the gastric area (wall and/or omentum), and 3.1% had nodules in the small intestine. All the samples had a moderate to severe lymphoplasmacytic infiltrate. Mild eosinophil infiltration was observed in both types of lesions, while neutrophil infiltration was significatively higher in the patchy than in the diffuse lesions. Fibrosis with mature collagen fibers was also predominant in the patchy lesions along with the presence of T lymphocytes and macrophages. Both the patchy and diffuse patterns had very few B lymphocytes. These findings suggest that the diffuse form is an earlier stage of the lesion, which eventually evolves into patchy forms. Neoplastic forms were not seen. Although more studies are necessary, this study describes the lesions, characterizes the inflammatory infiltrates, and establishes a possible evolution of the different pathological forms of S. vulpis infection in the red fox.
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http://dx.doi.org/10.7589/JWD-D-20-00162DOI Listing
November 2021

Histological and Immunohistochemical Study of Wounds in Sheep Skin in Maggot Therapy by Using Protophormia terraenovae (Diptera: Calliphoridae) Larvae.

J Med Entomol 2020 02;57(2):369-376

Animal Medicine Department, Pathological Anatomy Unit, University of Extremadura. Avenida de la Universidad SN. Cáceres, España.

A study was performed to evaluate the implication of Protophormia terraenovae larvae as a surgical therapy for wounded skin. Three groups of sheep (n = 25) were considered based on larval doses. Groups 1 and 2 were artificially infested with low and high concentrations of L1 stage P. terraenovae, respectively, and group 0 served as a control. Skin biopsies were taken at 4 and 14 d postinfestation (D.P.If). A histopathological study was carried out to evaluate the lesions with a score, numbers of eosinophils and mast cells, and an immunohistochemical analysis of CD3, CD79α, and CD68 as T lymphocytes, B lymphosytes, and macrophages, respectively. The results indicated that higher larval doses led to faster regeneration by 14 D.P.If. Furthermore, the higher larval doses showed a high number of the CD68 marker and eosinophils and a low number of CD3 and CD79α markers and mast cells. In addition, the number of mast cells, T lymphocytes, and macrophage markers increased when the lesion progressed; however, a low number of immunolabeled CD79α cells and eosinophils were observed. The results indicate a possible positive effect of larvae in the healing of certain wounds.
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http://dx.doi.org/10.1093/jme/tjz185DOI Listing
February 2020

Exploring the importance of mixed autogenous vaccines as a potential determinant of lung consolidation in lambs using Bayesian networks.

Prev Vet Med 2019 Aug 22;169:104693. Epub 2019 May 22.

Histology and Pathological Anatomy unit, Department of Animal Medicine, Faculty of Veterinary Medicine, University of Extremadura, Avda. de la Universidad s/n, 10003, Cáceres, Spain; Biotechnology Research Institute in Livestock & Cinegetic, Avda. de la Universidad s/n, 10003, Cáceres, Spain. Electronic address:

Bayesian networks are used to evaluate the effectiveness of mixed autogenous vaccines in fattening lambs to prevent the ovine respiratory syndrome. An experiment was performed with 460 fattening lambs, which were clustered into four groups according to the kind of vaccine received (Pasteurella spp., Mycoplasma spp., Mixed Mycoplasma-Pasteurella or placebo). After slaughtering, lungs were collected, and macroscopic and microscopic studies were performed. A microbiological study was carried out to evaluate the presence of Mycoplasma spp. and Pasteurellaceae by conventional culture and identification by nested polymerase chain reaction. To the best of the authors' knowledge, Bayesian networks have not been used to evaluate the effect of vaccines on the absence/presence of lung consolidation. Our results revealed that the use of mixed autogenous vaccines can decrease lung consolidation from 15.75% (12.42-19.08) to 9.24% (6.59-11.89). Therefore, the use of these autogenous vaccines in farms could be considered an effective control tool against ovine respiratory syndrome.
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http://dx.doi.org/10.1016/j.prevetmed.2019.104693DOI Listing
August 2019

Vaccination Against Porcine Circovirus-2 Reduces Severity of Tuberculosis in Wild Boar.

Ecohealth 2018 06 9;15(2):388-395. Epub 2018 Mar 9.

Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.

Tuberculosis (TB) in wild boar (Sus scrofa) may be affected by coinfections with other pathogens, such as porcine circovirus type 2 (PCV2). Therefore, sanitary measures focused on controlling PCV2 could be useful in reducing the impact of TB in this wild suid. The aim of this study was to explore whether vaccination against PCV2 targeting young animals affects TB prevalence and TB severity in wild boar. The study was conducted on a game estate in mid-western Spain. Seventy animals of ages ranging from 4 to 8 months were captured, individually identified, vaccinated against PCV2 and released, forming a vaccinated group. Not-captured animals cohabiting with the vaccinated wild boar constituted the control group. Animals from both groups were hunted between 2013 and 2016 and a TB diagnosis based on pathological assessment and microbiological culture was made in all of them. The effect of PCV2 vaccination on TB prevalence and severity was explored using generalized lineal models. Whereas TB prevalence was similar in vaccinated and control groups (54.55 vs. 57.78%), vaccinated animals showed less probabilities to develop generalized TB lesions. Furthermore, mean TB severity score was significantly lower in vaccinated animals (1.55 vs. 2.42) suggesting a positive effect of PCV2 vaccination.
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http://dx.doi.org/10.1007/s10393-018-1321-xDOI Listing
June 2018

and Serotypes Isolated from Merino Breed Lambs in Extremadura (Southwestern Spain).

Indian J Microbiol 2016 Dec 5;56(4):513-515. Epub 2016 Jul 5.

Infectious Diseases Unit, Department of Animal Health, Faculty of Veterinary Medicine, University of Extremadura, Avda de la Universidad s/n, 10003 Cáceres, Spain.

Pneumonia caused by is an important disease in ruminants. Because of its economic significance, several methods have been developed to study the pathogenicity and epidemiology of . In this study, bacterial isolates of and identified from the lungs of sheep were serotyped by means of indirect haemagglutination. Of the 598 lungs studied, 34 isolates were identified and serotyped. In decreasing order, serotypes were: not typable (50 %), A1 (17.65 %), A7 (11.76 %), A6 (5.88 %), and A12, A2, A5 and A9 (each representing 2.94 %). The only serotype was T4 (2.94 %). Serotypes A1, A6 and A7 of were the most commonly isolated from pneumonic sheep producing greater changes in the lungs and having important implications for sheep production.
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http://dx.doi.org/10.1007/s12088-016-0611-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061701PMC
December 2016

Investigations into the seasonal presence of Mycoplasma species in fattening lambs.

Vet J 2016 Jun 14;212:80-2. Epub 2016 Apr 14.

Histology and Pathological Anatomy, Department of Animal Medicine, Faculty of Veterinary Medicine, University of Extremadura, Avda de la Universidad s/n, Cáceres 10003, Spain; Research Institute for Biotechnology Livestock & Cynegetic, Avda de la Universidad s/n, Cáceres, Spain.

The presence of infection with Mycoplasma species in association with lung consolidation, environmental temperature and relative humidity was investigated in 410 clinically healthy fattening lambs from five different feedlots in Extremadura (southwestern Spain). Isolates of Mycoplasma species were obtained (n= 117), including Mycoplasma ovipneumoniae (n = 18) and Mycoplasma arginini (n = 99). Two seasonal periods were identified. The first period, which included February, March, September, October, and November, had an average temperature of 17.5 ± 4.7 °C and a relative humidity of 61.3 ± 15.8%. The second seasonal period, which included the months from April to August, had an average temperature of 22.9 ± 5.5 °C and a relative humidity of 48.4 ± 10.7%. Most Mycoplasma species were isolated from the second seasonal period, indicating that higher temperatures and lower relative humidity favour the presence of Mycoplasma species. M. arginini was also associated with lung consolidation.
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http://dx.doi.org/10.1016/j.tvjl.2016.04.006DOI Listing
June 2016
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