Publications by authors named "Jasmin Hassan"

9 Publications

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Persistent organic pollutants and the size of ovarian reserve in reproductive-aged women.

Environ Int 2021 May 1;155:106589. Epub 2021 May 1.

Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet and Karolinska University Hospital Huddinge, 141 86 Stockholm, Sweden. Electronic address:

Industrial chemicals such as persistent organic pollutants (POPs) have been associated with reduced fertility in women, including longer time-to-pregnancy (TTP), higher odds for infertility, and earlier reproductive senescence. Fertility is highly dependent on the ovarian reserve, which is composed of a prenatally determined stock of non-growing follicles. The quantity and quality of the follicles decline with age, thereby eventually leading to menopause. In the clinical setting, assessing ovarian reserve directly through the histological analysis of follicular density in ovaries is not practical. Therefore, surrogate markers of ovarian reserve, such as serum anti-Müllerian hormone (AMH) are typically used. Here, we studied associations between chemical exposure and ovarian reserve in a cohort of pregnant women undergoing elective caesarean section (n = 145) in Stockholm, Sweden. Full data (histological, clinical, serum) were available for 50 women. We estimated the size of the reserve both directly by determining the density of follicles in ovarian cortical tissue samples, and indirectly by measuring AMH in associated serum samples. Concentrations of 9 organochlorine pesticides (OCPs), 10 polychlorinated biphenyls (PCBs), 3 polybrominated diphenylethers (PBDEs) and 9 perfluoroalkyl substances (PFAS) were determined in serum, and clinical data were retrieved from electronic medical records. Healthy follicle densities (median 0, range 0-193 follicles/mm) and AMH levels (median 2.33 ng/mL, range 0.1-14.8 ng/mL) varied substantially. AMH correlated with the density of growing follicles. Twenty-three chemicals detected in more than half of the samples were included in the analyses. None of the chemicals, alone or as a mixture, correlated with AMH, growing or atretic follicles. However, HCB, transnonachlor, PCBs 74 and 99 were associated with decreased non-growing follicle densities. HCB and transnonachlor were also negatively associated with healthy follicle density. Further, mixture of lipophilic POPs (PBDE 99, p,p'-DDE, and PCB 187) was associated with lower non-growing follicle densities. In addition, exposure to HCB, p,p'-DDE, and mixture of OCPs were significantly associated with higher odds of infertility. The results suggest that exposure to chemicals may reduce the size of ovarian reserve in humans, and strongly encourage to study mechanisms behind POP-associated infertility in women in more detail.
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http://dx.doi.org/10.1016/j.envint.2021.106589DOI Listing
May 2021

Fertility Preservation for Prepubertal Patients at Risk of Infertility: Present Status and Future Perspectives.

Horm Res Paediatr 2020 22;93(11-12):599-608. Epub 2021 Apr 22.

Childhood Cancer Research Unit, Department of Women's and Children's Health, NORDFERTIL Research Laboratory Stockholm, Karolinska Institutet, and Karolinska University Hospital, Stockholm, Sweden.

The increasing cure rate of cancer has led to a vast population of survivors having to face the late adverse effects of oncological treatments, with fertility impairment being one of the most sensitive issues for patients. Different options to preserve the fertility of adult patients are routinely used in clinical practice. However, fertility preservation strategies for prepubertal patients at risk of infertility are limited to the cryopreservation of immature gonadal tissue. In recent decades, many research efforts have been focused on the future use of cryopreserved gonadal tissue. This review discusses the common status of fertility preservation measures for pediatric patients undergoing gonadotoxic treatment, focusing especially on the challenges that remain to be solved in order to implement this fundamental service.
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http://dx.doi.org/10.1159/000516087DOI Listing
April 2021

Predilection of Low Protein C-induced Spontaneous Atherothrombosis for the Right Coronary Sinus in Apolipoprotein E deficient mice.

Sci Rep 2018 10 10;8(1):15106. Epub 2018 Oct 10.

Division of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands.

Silencing of anticoagulant protein C using RNA interference (siProc) evokes low incident but spontaneous atherothrombosis in the aortic root of apolipoprotein E-deficient (Apoe) mice. The aims of the current study were (1) to analyze if plaque characteristics or circulating factors could be linked to atherothrombosis susceptibility, (2) to increase the incidence of atherothrombosis by transiently increasing blood pressure, and (3) to direct atherothrombosis to an additional predefined vascular site by applying a semi-constrictive collar around the carotid artery. siProc-driven spontaneous atherothrombosis in the aortic root of Apoe mice was reproduced and occurred at an incidence of 23% (9 out of 39 mice), while the incidence of collar-induced atherothrombosis in the carotid artery was 2.6% (1 out of 39 mice). Treatment with phenylephrine, to transiently increase blood pressure, did not increase atherothrombosis in the aortic root of the Apoe mice nor in the carotid arteries with collars. Plaques in the aortic root with an associated thrombus were lower in collagen and macrophage content, and mice with atherothrombosis had significantly more circulating platelets. Plasma protein C, white blood cell counts, total cholesterol, fibrinogen, serum amyloid A, and IL-6 were not different amongst siProc treated mice with or without thrombosis. Remarkably, our data revealed that thrombus formation preferably occurred on plaques in the right coronary sinus of the aortic root. In conclusion, there is a predilection of low protein C-induced spontaneous atherothrombosis in Apoe mice for the right coronary sinus, a process that is associated with an increase in platelets and plaques lower in collagen and macrophage content.
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http://dx.doi.org/10.1038/s41598-018-32584-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180072PMC
October 2018

Hypercholesterolemia impairs megakaryopoiesis and platelet production in scavenger receptor BI knockout mice.

Atherosclerosis 2019 03 22;282:176-182. Epub 2018 Sep 22.

Division of BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the Netherlands.

Background And Aims: Thrombocytopenia in scavenger receptor BI (SR-BI) knockout mice is suggested to result from augmented platelet clearance induced by elevated intracellular unesterified cholesterol (UC) levels. We hypothesize that SR-BI deficiency may also influence platelet production at the level of its precursor cell in the bone marrow, the megakaryocyte.

Methods: In this study, we compared megakaryopoiesis and platelet production in SR-BI knockout and wild-type mice.

Results: In line with our hypothesis, megakaryocytes from SR-BI knockout mice exhibited UC accumulation while no accumulation of UC was detectable in wild-type megakaryocytes. Bone marrow expression of transcription factors involved in megakaryocyte maturation was induced, but megakaryocyte counts were unchanged in bone marrow of SR-BI knockout mice. Interestingly, we did find a striking 62% decrease (p < 0.01) in proplatelet production by SR-BI knockout megakaryocytes. SR-BI knockout mice displayed an impaired increase in circulating platelet concentrations and bone marrow megakaryocyte numbers upon thrombopoietin challenge. Importantly, megakaryocytes from normolipidemic bone marrow-specific SR-BI knockout mice exhibited a normal ability to produce proplatelets. Moreover, bone marrow-specific deletion of SR-BI did not impair the thrombopoietin response or induce thrombocytopenia, confirming that absence of megakaryocyte SR-BI does not underlie the thrombocytopenic phenotype in total body SR-BI knockout mice.

Conclusions: In conclusion, the elevation of plasma unesterified cholesterol levels impairs megakaryopoiesis and platelet production in SR-BI knockout mice. Our findings suggest that, in addition to an increased platelet clearance, a decrease in platelet production may also, in part, explain the thrombocytopenic phenotype associated with SR-BI deficiency in mice.
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http://dx.doi.org/10.1016/j.atherosclerosis.2018.09.019DOI Listing
March 2019

Long-Term Changes in Backscattered Light Measurements in Keratoconus Corneas Treated with Collagen Cross-Linking.

Curr Eye Res 2018 01 7;43(1):18-26. Epub 2017 Nov 7.

b Orbi-Dent Refractive Surgery and Medical Center , Debrecen , Hungary.

Purpose: Our aim was to compare densitometry data between keratoconus and normal corneas and to assess the long-term changes in corneal backscattered light values after corneal collagen cross-linking (CXL) treatment.

Material And Methods: Retrospective analysis was performed on 26 eyes of normal patients (age: 32.71 ± 12.68 years) and 39 eyes of keratoconus patients (28.93 ± 7.59 years) using the Pentacam HR corneal densitometry module before CXL and during the postoperative period, which lasted a mean of 2.53 years (range: 1-4 years). Corneal backscattered light values in grey scale unit (GSU) were recorded for the anterior 120 µm, the center layer, and the posterior 60 µm of the cornea in four concentric, central rings. An additional calculation was performed with converting GSU values to GSU/cubic millimeters (mm) with surface area calculations and using corneal thickness data.

Results: Statistically significant differences were observed between normal and keratoconus group densitometry values (in GSU/mm) in all annuli of the center layer; all, but anterior layer of the 10-12 annuli; all annuli of the total thickness and in all values of the total diameter. In all these cases, the normal cornea showed higher GSU/mm values compared to keratoconus corneas (p < 0.05). One month after the CXL treatment, the GSU/mm data increased significantly in the anterior, the center, and the posterior zone (p < 0.05), followed by its slow decrease. At the end of the follow-up period, the GSU/mm values were significantly higher in all three zones compared to those before the CXL treatment (p < 0.01).

Conclusion: Densitometry measurements can reflect the changes of optical quality of the cornea. These measurements may play a valuable role in assessing keratoconus and optical changes of the corneas after CXL treatment.
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http://dx.doi.org/10.1080/02713683.2017.1377260DOI Listing
January 2018

Uterine disorders in 50 pet rabbits.

J Am Anim Hosp Assoc 2015 Jan-Feb;51(1):8-14. Epub 2014 Nov 21.

From the Clinic of Internal Medicine and Infectious Diseases (F.K., S.S.), Clinic for Diagnostic Imaging (J.H.), Clinic for Obstetrics, Gynecology and Andrology (P.B.), Institute of Pathology and Forensic Medicine (A.F-B.), and Institute of Medical Physics and Biostatistics, Department of Natural Science (A.T.), University of Veterinary Medicine, Vienna, Austria; Veterinary Practice Eggenberg, Graz, Austria (P.G.).

Although the incidence of uterine disorders in pet rabbits is high there are only a few retrospective studies and case reports on genital tract disease in female rabbits. Uterine disorders were assessed in 50 pet rabbits. In 31 pet rabbits with suspected clinical uterine disease, medical records were further reviewed regarding clinical signs, diagnostic workup, treatment as well as the outcome itself. Uterine adenocarcinoma (54%) was most frequently diagnosed, followed by endometrial hyperplasia (26%). Serosanguineous vaginal discharge was the predominant clinical sign observed by the rabbit owners. In approximately 50% of the rabbits with suspected uterine disorders, abdominal palpation revealed enlarged and/or irregular masses in the caudoventral abdomen indicating uterine lesions. Out of 23 rabbits undergoing ovariohysterectomy, four were either euthanized or died shortly after surgery because they were clinically unstable. Overall, 80% of the ovariohysterectomized animals were still alive 6 mo after surgery. In female pet rabbits that are not breeding, either ovariohysterectomy should be performed at an early age or routine checks including ultrasonography of the abdomen are recommended on a regular basis.
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http://dx.doi.org/10.5326/JAAHA-MS-5812DOI Listing
August 2016

Thymomas in rabbits: clinical evaluation, diagnosis, and treatment.

J Am Anim Hosp Assoc 2012 Mar-Apr;48(2):97-104. Epub 2012 Jan 19.

Clinical Department for Small Animals and Horses, University of Veterinary Medicine, Vienna, Austria.

Thymomas are rarely recorded in rabbits, and the literature includes comparatively few cases. Medical records were reviewed to identify all pet rabbits in which a mediastinal mass was diagnosed between Feb 2007 and Jan 2010. Signalment, history, clinical signs, diagnostic work-up (including laboratory data, diagnostic imaging, and ultrasound-guided fine-needle aspiration of the mediastinal mass), treatment modalities, survival time, and histologic findings were evaluated. Cytologic and/or histopathologic examinations revealed thymomas in all rabbits with mediastinal masses (n=13). Rabbits with thymomas showed clinical signs of dyspnea (76.9%), exercise intolerance (53.9%), and bilateral exophthalmos (46.2%). In seven rabbits the thymoma was removed surgically. Two rabbits were treated conservatively, and four rabbits were euthanized because of their poor clinical condition. The two rabbits that underwent surgery were euthanized 6 mo and 34 mo later. Mediastinal masses in rabbits appear to be more common than previously believed and consist primarily of thymomas rather than thymic lymphomas. Cytology of samples collected by ultrasound-guided fine-needle aspiration is an accurate diagnostic tool for the identification of thymomas in rabbits. Due to a high rate of perioperative mortality, intensive perioperative care and the provision of a low-stress environment are recommended for a successful thoracotomy.
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http://dx.doi.org/10.5326/JAAHA-MS-5683DOI Listing
April 2012

Clinical symptoms and diagnosis of encephalitozoonosis in pet rabbits.

Vet Parasitol 2008 Feb 17;151(2-4):115-24. Epub 2007 Nov 17.

Clinic of Internal Medicine and Infectious Diseases, Clinical Department of Small Animals and Horses, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria.

Infections with Encephalitozoon cuniculi in rabbits are observed at increasing frequency and are known as opportunistic infections in immunocompromised humans. 191 pet rabbits with suspected encephalitozoonosis, presented at the Animal Hospital of the Veterinary University of Vienna (Austria), were included in this study. Rabbits were serologically examined for antibodies against E. cuniculi (144 positive out of 184 rabbits with suspected encephalitozoonosis compared to 14 positive out of 40 clinically healthy rabbits tested as part of a standard health check) and Toxoplasma gondii (8 positive out of 157). Of the 144 seropositive rabbits with clinical signs, 75% showed neurological symptoms, 14.6% demonstrated phacoclastic uveitis and 3.5% suffered from renal failure. 6.9% of the animals had combined symptoms. Vestibular disease dominated within the rabbits that showed neurological symptoms. Polymerase chain reaction (PCR) could not detect parasite DNA in urine or cerebrospinal fluid (CSF), but did so in 4 out of 5 samples of liquefied lens material in cases with phacoclastic uveitis due to lens capsule rupture. Additionally further diagnostic procedures, such as inspection of the external ear canal (N=69), radiography of the tympanic bullae (N=65) were performed to rule out differential diagnosis. 54.2% of the patients exhibiting neurological symptoms recovered within a few days, while 87.5% of the rabbits suffering from renal failure died or had to be euthanized.
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http://dx.doi.org/10.1016/j.vetpar.2007.11.005DOI Listing
February 2008

Malignant B-cell lymphoma of the Harder's gland in a rabbit.

Vet Ophthalmol 2005 Jul-Aug;8(4):259-63

Department of Small Animals and Horses, University of Veterinary Medicine of Vienna, Vienna, Austria.

A 22-month-old, female rabbit was presented with a 1-day history of acute unilateral exophthalmos. Ultrasonography and computed tomography (CT) of the orbit revealed an orbital mass. Retrobulbar lymphoma was diagnosed following fine-needle aspiration biopsy (FNAB). Thoracic radiographs were normal, and ultrasonography of the abdomen showed focal hypoechoic thickening of the bowel wall and hypoechoic enlarged lymph nodes. The rabbit was euthanized and histopathology identified the retrobulbar mass as B-cell malignant lymphoma of the Harder's gland. Mesenteric lymph nodes, caecum, and both kidneys were also affected. This is the first documented case of malignant lymphoma of the Harder's gland in a rabbit.
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http://dx.doi.org/10.1111/j.1463-5224.2005.00400.xDOI Listing
September 2005