Publications by authors named "Jamshid Amiri Moghaddam"

11 Publications

  • Page 1 of 1

Intra and extracellular effects of benzo [α] pyrene on liver, gill and blood of Caspian White fish (Rutilus frissi kutum): Cyto-genotoxicity and histopathology approach.

Mar Pollut Bull 2021 Feb 7;163:111942. Epub 2021 Jan 7.

Leibniz Institute for Natural Product Research and Infection Biology e.V. Hans-Knöll-Institute (HKI), Jena, Germany.

Southern parts of the Caspian Sea have been faced with a diverse range of oil pollutants. Therefore, this study was designed to evaluate the effects of relevant environmental concentrations of benzo[α]pyrene (BαP) on liver, gill, and blood of Caspian White fish. To this end, 150 fingerling fish (6.5 ± 0.8 g) were exposed to under, near and over environmental concentrations of BαP (i.e. 50, 100, and 200 ppb, respectively) and two control groups for 21 days. Following exposure to BαP, generally, DNA damage increased in the liver and gill cells as well as the frequency of micro- and bi-nucleated erythrocytes in a time and concentration-dependent pattern. In addition, the liver and gill tissues displayed several histopathological lesions. Together, the findings are warning the health status of the Caspian Sea due to an ever-increasing concentration of BαP through using Caspian White fish as an ecological model.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111942DOI Listing
February 2021

Recent highlights of biosynthetic studies on marine natural products.

Org Biomol Chem 2021 01;19(1):123-140

Junior Research Group Chemical Biology of Microbe-Host Interactions, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Beutenbergstr. 11a, 07745 Jena, Germany.

Marine bacteria are excellent yet often underexplored sources of structurally unique bioactive natural products. In this review we cover the diversity of marine bacterial biomolecules and highlight recent studies on structurally novel natural products. We include different compound classes and discuss the latest progress related to their biosynthetic pathway analysis and engineering: examples range from fatty acids over terpenes to PKS, NRPS and hybrid PKS-NRPS biomolecules.
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http://dx.doi.org/10.1039/d0ob01677bDOI Listing
January 2021

From Persian Gulf to Indonesia: interrelated phylogeographic distance and chemistry within the genus Peronia (Onchidiidae, Gastropoda, Mollusca).

Sci Rep 2020 08 3;10(1):13048. Epub 2020 Aug 3.

Zoologisches Forschungsmuseum Alexander Koenig, Bonn, Germany.

The knowledge of relationships between taxa is essential to understand and explain the chemical diversity of the respective groups. Here, twelve individuals of the panpulmonate slug Peronia persiae from two localities in Persian Gulf, and one animal of P. verruculata from Bangka Island, Indonesia, were analyzed in a phylogenetic and chemotaxonomic framework. Based on the ABGD test and haplotype networking using COI gene sequences of Peronia specimens, nine well-supported clades were found. Haplotype network analysis highlighted a considerable distance between the specimens of P. persiae and other clades. Metabolomic analysis of both species using tandem mass spectrometry-based GNPS molecular networking revealed a large chemical diversity within Peronia of different clades and localities. While P. persiae from different localities showed a highly similar metabolome, only few identical chemical features were found across the clades. The main common metabolites in both Peronia species were assigned as polypropionate esters of onchitriols and ilikonapyrones, and osmoprotectant amino acid-betaine compounds. On the other hand, the isoflavonoids genistein and daidzein were exclusively detected in P. persiae, while cholesterol and conjugated chenodeoxycholic acids were only found in P. verruculata. Flavonoids, bile acids, and amino acid-betaine compounds were not reported before from Onchidiidae, some are even new for panpulmonates. Our chemical analyses indicate a close chemotaxonomic relation between phylogeographically distant Peronia species.
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http://dx.doi.org/10.1038/s41598-020-69996-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400755PMC
August 2020

and Venom: A New Source of Conopeptides with Analgesic Activity.

Avicenna J Med Biotechnol 2020 Jul-Sep;12(3):179-185

Institute for Pharmaceutical Biology, University of Bonn, Bonn, Germany.

Background: Cone snails are a natural source of complex peptides with analgesic properties called conotoxins. These peptides are secreted in a complex venomic mixture and are predominantly smaller than 5 . The present study aimed to document the analgesic activity of two species of () and () venom collected off the Iranian coast in a mouse behavioral test.

Methods: Conotoxin containing fractions was extracted from the venom ducts and initially purified by column chromatography. The analgesic effect of the fractions was determined on formalin pain model and hot-plate test.

Results: The results led to the identification of four fractions with analgesic activity in and two in . Only one fraction was able to reduce the flinching and licking in both acute pain and chronic pain phases of the formalin test. Moreover, the activity of this fraction remained 30 minutes on the hot-plate test. Purification of the fractions was carried out by RP-HPLC. LC-ESI-MS analysis of the fractions showed that the conotoxins of the analgesic fraction had molecular weights not previously reported.

Conclusion: The findings give insight into the venom of two previously under-investigated species and reveal the therapeutic potential of the containing conopeptides.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368110PMC
July 2020

Immunomodulatory function of antimicrobial peptide EC-Hepcidin1 modulates the induction of inflammatory gene expression in primary cells of Caspian Trout (Salmo trutta caspius Kessler, 1877).

Fish Shellfish Immunol 2020 Sep 28;104:55-61. Epub 2020 May 28.

Leibniz Institute for Natural Product Research and Infection Biology e.V. Hans-Knöll-Institute (HKI), Jena, Germany. Electronic address:

Hepcidins, a group of antimicrobial peptides (AMPs), play a key role in the innate immune system of fishes and act against different pathogens. In this study, antimicrobial and immune-inflammatory activity of a synthetic EC-hepcidin1, previously identified from orange-spotted grouper, were evaluated. EC-hepcidin1 showed weak activity against the zoonotic fish pathogen Streptococcus iniae (MIC 100 μg mL and MBC 150 μg mL). To study the effect of AMPs in general, and EC-hepcidin1 in particular, a primary cell culture (SC) from the fin tissue of the Caspian Trout (Salmo trutta caspius) was established. The neutral Red method on SC cells revealed that EC-hepcidin1 has no or very low cytotoxic properties. Treatment of cells with either EC-hepcidin1 (150 μg mL) or fish pathogen Streptococcus iniae (MOI = 10) and a mixture of both resulted in the up-regulation of gene expression of MHC-UBA, IL-6, and TNFα indicating the modulatory function on inflammatory processes. These findings indicate that EC-hepcidin1 might act as a candidate for modulation of the innate immune system in S. iniae-based infection.
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http://dx.doi.org/10.1016/j.fsi.2020.05.067DOI Listing
September 2020

Analysis of the Genome and Metabolome of Marine Myxobacteria Reveals High Potential for Biosynthesis of Novel Specialized Metabolites.

Sci Rep 2018 11 9;8(1):16600. Epub 2018 Nov 9.

German Center for Infection Research (DZIF) Partner Site Cologne/Bonn, Bonn, Germany.

Comparative genomic/metabolomic analysis is a powerful tool to disclose the potential of microbes for the biosynthesis of novel specialized metabolites. In the group of marine myxobacteria only a limited number of isolated species and sequenced genomes is so far available. However, the few compounds isolated thereof so far show interesting bioactivities and even novel chemical scaffolds; thereby indicating a huge potential for natural product discovery. In this study, all marine myxobacteria with accessible genome data (n = 5), including Haliangium ochraceum DSM 14365, Plesiocystis pacifica DSM 14875, Enhygromyxa salina DSM 15201 and the two newly sequenced species Enhygromyxa salina SWB005 and SWB007, were analyzed. All of these accessible genomes are large (~10 Mb), with a relatively small core genome and many unique coding sequences in each strain. Genome analysis revealed a high variety of biosynthetic gene clusters (BGCs) between the strains and several resistance models and essential core genes indicated the potential to biosynthesize antimicrobial molecules. Polyketides (PKs) and terpenes represented the majority of predicted specialized metabolite BGCs and contributed to the highest share between the strains. BGCs coding for non-ribosomal peptides (NRPs), PK/NRP hybrids and ribosomally synthesized and post-translationally modified peptides (RiPPs) were mostly strain specific. These results were in line with the metabolomic analysis, which revealed a high diversity of the chemical features between the strains. Only 6-11% of the metabolome was shared between all the investigated strains, which correlates to the small core genome of these bacteria (13-16% of each genome). In addition, the compound enhygrolide A, known from E. salina SWB005, was detected for the first time and structurally elucidated from Enhygromyxa salina SWB006. The here acquired data corroborate that these microorganisms represent a most promising source for the detection of novel specialized metabolites.
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http://dx.doi.org/10.1038/s41598-018-34954-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226438PMC
November 2018

Cyclopropane-Containing Fatty Acids from the Marine Bacterium sp. 011 with Antimicrobial and GPR84 Activity.

Mar Drugs 2018 Oct 8;16(10). Epub 2018 Oct 8.

Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany.

Bacteria of the family Rhodobacteraceae are widespread in marine environments and known to colonize surfaces, such as those of e.g., oysters and shells. The marine bacterium sp. 011 is here investigated and it was found to produce two cyclopropane-containing medium-chain fatty acids (, ), which inhibit the growth of a range of bacteria and fungi, most effectively that of a causative agent of Roseovarius oyster disease (ROD), DSM 16950. Additionally, compound acts as a potent partial, β-arrestin-biased agonist at the medium-chain fatty acid-activated orphan G-protein coupled receptor GPR84, which is highly expressed on immune cells. The genome of sp. 011 was sequenced and bioinformatically compared with those of other spp. This analysis revealed several cyclopropane fatty acid synthases (CFAS) conserved in all strains analyzed and a putative gene cluster encoding for two distinct CFASs is proposed as the biosynthetic origin of and .
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http://dx.doi.org/10.3390/md16100369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213206PMC
October 2018

Draft Genome Sequences of the Obligatory Marine Myxobacterial Strains Enhygromyxa salina SWB005 and SWB007.

Genome Announc 2018 Apr 26;6(17). Epub 2018 Apr 26.

Institute for Insect Biotechnology, Justus Liebig University Giessen, Giessen, Germany

The two marine myxobacterial strains SWB005 and SWB007 were isolated from coastal soil samples using as bait for these predatory strains. These strains produce unique specialized metabolites. Genomes were assembled into 312 contigs for SWB005 (9.0 Mbp) and 192 contigs for SWB007 (10.6 Mbp).
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http://dx.doi.org/10.1128/genomeA.00324-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5920174PMC
April 2018

Different strategies of osmoadaptation in the closely related marine myxobacteria SWB007 and SIR-1.

Microbiology (Reading) 2016 Apr;162(4):651-661

Institute for Pharmaceutical Biology, University of Bonn, Nussallee 6, 53115 Bonn, Germany.

Only a few myxobacteria are known to date that are classified as marine, owing to their salt dependency. In this study, the salt tolerance mechanism of these bacteria was investigated. To this end, a growth medium was designed in which the mutated strain BKA13 served as sole food source for the predatory, heterotrophic myxobacteria. This enabled measurement of the osmolytes without any background and revealed that the closely related strains SWB007 and SIR-1 developed different strategies to handle salt stress. SIR-1, which was grown between 1 and 4 % NaCl, relies solely on the accumulation of amino acids, while SWB007, which was grown between 0.5 and 3 % NaCl, employs, besides betaine, hydroxyectoine as the major compatible solute. In accordance with this analysis, only in the latter strain was a locus identified that codes for genes corresponding to the biosynthesis of betaine, ectoine and hydroxyectoine.
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http://dx.doi.org/10.1099/mic.0.000250DOI Listing
April 2016

Effect of gamma radiation on the growth, survival, hematology and histological parameters of rainbow trout (Oncorhynchus mykiss) larvae.

Aquat Toxicol 2015 Aug 24;165:259-65. Epub 2015 Jun 24.

Fisheries Department, Faculty of Natural Resources and Marine Sciences, TarbiatModares University, Noor, Mazandaran, Iran.

Effects of low (1, 2.5 and 5Gy) and high doses (10, 20 and 40Gy) of gamma radiation were examined on the growth, survival, blood parameters and morphological changes of the intestines of rainbow trout (Oncorhynchus mykiss) larvae (103±20mg) after 12 weeks of exposure. Negative effects of gamma radiation on growth and survival were observed as radiation level and time increased. Changes were well documented at 10 and 20Gy. All the fish were dead at the dose of 40Gy. In all the treatments, levels of red blood cells (RBC), hematocrit (HCT) and hemoglobin (HB) were significantly (P<0.05) declined as the irradiation levels increased, whereas the amount of mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) did not change. No significant differences (P>0.05) were found in the levels of white blood cells (WBC), lymphocytes and monocytes. Destruction of the intestinal epithelium cells was indicated as the irradiation levels increased to 1Gy and above. The highest levels of growth, survival, specific growth rate (SGR), condition factor (CF) and protein efficiency rate (PER) were obtained in the control treatment. The results showed that gamma rays can be a potential means for damaging rainbow trout cells.
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http://dx.doi.org/10.1016/j.aquatox.2015.06.010DOI Listing
August 2015
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